CN102415369A - Eupatorium odoratum standard sample and preparation method thereof - Google Patents
Eupatorium odoratum standard sample and preparation method thereof Download PDFInfo
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- CN102415369A CN102415369A CN2011102207845A CN201110220784A CN102415369A CN 102415369 A CN102415369 A CN 102415369A CN 2011102207845 A CN2011102207845 A CN 2011102207845A CN 201110220784 A CN201110220784 A CN 201110220784A CN 102415369 A CN102415369 A CN 102415369A
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Abstract
The invention relates to an Eupatorium odoratum standard sample and a preparation method thereof, and belongs to the technical field of research and development on plant quarantine standard sample. In the Eupatorium odoratum standard sample of the invention, a confirmed Eupatorium odoratum is inlayed in transparent resin, with one Eupatorium odoratum in each resin. According to the invention, Eupatorium odoratum standard sample is inlayed in transparent resin to prepare a crystal specimen for three reasons. First, a resin raw material is liquid and with good fluidity, can fully fill a sample surface and will not dissolve into water after solidifying into a film; second, the resin has high degree of transparency, which is suitable for observing microstructures in the standard sample; third, the resin has excellent performances of flame retardation, aging resistance, non-toxicity, antioxidation, heat resistance and insulation, etc. Accordingly, the invention inlays Japanese semen cuscutae standard sample in the resin to prolong an effective stage of the standard sample and reduce cost of the standard sample.
Description
Technical field
The present invention relates to the fragrant eupatorium herb standard sample, the invention still further relates to its preparation method in addition, belong to plant quarantine standard sample technical research field.
Background technology
Fragrant eupatorium herb (
Eupatorlum odoratumL.), feverfew.Be born in the torrid zone, semi-tropical hillside, roadside.Fragrant eupatorium herb secretion allelochemical squeezes eciophyte, makes the grassland lose value, influences tree growth and renewal.Fragrant eupatorium herb influences the growth of cereal crops, mulberry tree, Chinese prickly ash, banana etc., reduces output and stops up water channel, blocks the traffic.Cause wild rare traditional Chinese medicine to lose living environment.Leaf is poisonous, contains having of Coumarins and poisons and thing, causes people's scytitis and anaphylactia, eats tender leaf by mistake and causes dizziness, vomiting, causes that domestic animal, poultry and fish poison.Given this, China Ministry of Agriculture as important quarantine object, is listed fragrant eupatorium herb in " People's Republic of China (PRC) enter the territory plant quarantine property pest register ".Disinfection in the inspection and quarantine process of food import and seed, in case find to contain fragrant eupatorium herb, must be done by inspection and quarantining for import/export department, and can be as the case may be, and the claim of externally coming to testify is for country retrieves economic losses.
At present, when China quarantine mechanism or laboratory were carried out the fragrant eupatorium herb quarantine and identified, the fragrant eupatorium herb standard sample majority of institute's reference was intercepted and captured from the external goods that enters the territory, or other relevant departments give, and also had plenty of to gather in the field.Because the fragrant eupatorium herb source is different, thus be used for having very big difference as its form of fragrant eupatorium herb of standard sample, thus influence quarantine qualification result.Current still do not have unified fragrant eupatorium herb standard sample in the world and be used for relevant department's evaluation of quarantining.So typical case on form is consistent in development, representative fragrant eupatorium herb standard sample is significant.
Summary of the invention
The purpose of this invention is to provide the fragrant eupatorium herb standard sample, uniformity is good with stability, promotes the quarantine evaluation work of plant quarantine laboratory, improves the laboratory detection level.The present invention also provides its preparation method in addition, and technology is simple, easy operating.
Fragrant eupatorium herb standard sample of the present invention is embedded in the transparent resin 1 fragrant eupatorium herb in every block of resin through the fragrant eupatorium herb of confirming.
The preparation method of fragrant eupatorium herb standard sample of the present invention, concrete steps are:
First step standard sample source: the fragrant eupatorium herb seed that the field is gathered carries out isolation implant in growth in season after identifying, according to whole plant and flowers and fruits characteristic it is further verified, confirms the authenticity of fragrant eupatorium herb;
The second step standard sample expands numerous: at first disinfecting soil is handled; Be contained in the soil that disinfects in the big flowerpot then, neatly put flowerpot again and isolate in the solarium, all its growing state has been carried out observation and record in the whole growth season of fragrant eupatorium herb; After the fragrant eupatorium herb maturation; Gather and airing, the fragrant eupatorium herb that airing is good is contained in the drier to be preserved under the room temperature, prepares to be used for the screening and the making of standard sample;
The 3rd step standard sample screening: at first the fragrant eupatorium herb seed is carried out inactivation treatment; Be poured on through the fragrant eupatorium herb seed of inactivation treatment and carry out primary dcreening operation in the white disk; The seed that select full seed, is of moderate size; The seed picked out is prepared to carry out dusting cover, pay close attention to the nuance of the identification of morphology characteristic between the seed individuality, will pass through the seed that dusting cover picks out and be put in the white port grinding bottle subsequent use respectively;
The 4th step, standard sample preparation:
(1) makes mould, jaqan evenly is sprayed on die surface;
(2) install external mold behind the cleaning mold;
(3) crystal resin is added the dustless inserts of nanometer;
(4) add batching such as curing agent, promoter, antifoaming agent, transparent masterbatches and stir well even;
(5) pour resin into mould after, shake mould, make in the mould resin flows even, mould is just put after evenly, prevent to be out of shape in the hardening of resin process;
(6) seed is put into resin;
(7) after resin solidifies fully, slough mould;
(8) with product polishing, polishing.
Said laggard column criterion sample homogeneity of standard sample and the stability test of making:
(1) uniformity testing: the picked at random standard sample divides into groups, and identifies through the expert respectively, and the expert assert that the nuance that exists between individuality to not influence of fragrant eupatorium herb identification of morphology result, is uniform between individuality on form;
(2) stability test: choose the fragrant eupatorium herb standard sample that 20 packings complete immediately; Respectively through naked eyes or use magnifying glass, anatomical lens that its external form is observed; And sample that itself and packing are handled compared; The two does not externally have difference on the form, explains that this sample stability is good.
Inactivation treatment is in said the 3rd step standard sample screening: seed is placed in 50 ℃ the insulating box, carries out seed germination experiment behind the 48h, confirm that seed loses vigor.
The present invention is embedded in the fragrant eupatorium herb standard sample in the resin, processes quartzy sample.Its reason has three, and first resin raw material is liquid, has extraordinary flowability, can fully be full of sample surfaces, and no longer water-soluble after the dried solid film forming.It two is that the resin transparent degree is high, is convenient to observe the fine structure of the inside standard sample.Its premium properties such as three to be that resin has fire-retardant, ageing-resistant, nontoxic, anti-oxidant, heat-resisting, insulation etc., reason to sum up, the present invention is embedded in Japanese dodder standard sample in the resin, has prolonged the term of validity of standard sample, has reduced the standard sample cost.The packing of fragrant eupatorium herb standard sample of the present invention adopts can long preservation and the resin material wear-resistant, that light transmission is good, and makes through special processing technology.Fragrant eupatorium herb standard sample preservation condition: normal temperature is preserved down, and the sunlight direct projection is avoided in protection against the tide.The term of validity is 5 years.
Preparation method's technology of fragrant eupatorium herb standard sample of the present invention is simple; The fragrant eupatorium herb seed of gathering with the field is a raw material; Through isolation implant, expand numerous, screening, preparation, uniformity testing, stability test, packing and storage supervisor and be prepared into representative fragrant eupatorium herb standard sample; For plant quarantine laboratory weeds quarantine evaluation work provides appraisal basis, improve the laboratory detection level.
Four, embodiment:
Below in conjunction with specific embodiment the present invention is done further explain, but the present invention is not limited to specific embodiment.
Embodiment 1
The preparation method of fragrant eupatorium herb standard sample, concrete steps are:
First step standard sample source: the fragrant eupatorium herb seed that the field is gathered carries out isolation implant in growth in season after identifying, according to whole plant and flowers and fruits characteristic it is further verified, confirms the authenticity of fragrant eupatorium herb;
The second step standard sample expands numerous: according to soil disinfection handle put in the flowerpot of packing into isolate that the solarium implants that the fragrant eupatorium herb seed is observed, record results, airing preserve technological process and expand numerous: according to the weather conditions of locality, select different seedtimes.At first disinfecting soil is handled, can be killed other seed and pathogen in the soil like this, be contained in the soil that disinfects in the big flowerpot then, neatly put flowerpot again and isolate in the solarium, and carry out sign.Whole growth season at fragrant eupatorium herb has all been carried out observation and record to its growing state, and takes into picture, prepares as the part in the standard sample technical data.After the fragrant eupatorium herb maturation, gather and airing.The fragrant eupatorium herb that airing is good is contained in the drier to be preserved under the room temperature, prepares to be used for the screening and the making of standard sample.In addition, the seed of from this batch fragrant eupatorium herb, selecting full seed is contained in and carries out sign in the envelope, expands numerous seed resource as fragrant eupatorium herb;
The 3rd step standard sample screening: before screening,, at first will carry out inactivation treatment to seed for guaranteeing bio-safety.The fragrant eupatorium herb seed is placed in 50 ℃ the insulating box, carries out seed germination experiment behind the 48h, confirm that seed loses vigor.To be poured on through the seed of inactivation treatment in the white disk and carry out primary dcreening operation.The seed that the primary dcreening operation process is mainly selected full seed, is of moderate size prepares to carry out dusting cover with the seed of picking out.The dusting cover process mainly is to combine sample homogeneity to test to accomplish.The dusting cover process will be paid close attention to the nuance of the identification of morphology characteristic between the seed individuality.To pass through the seed that dusting cover picks out and be put into respectively in the white port grinding bottle, subsequent use;
The standard sample preparation of the 4th step: concrete fragrant eupatorium herb standard sample work flow is following:
(1) makes mould, jaqan natural resin synthetic varnish evenly is sprayed on die surface;
(2) install external mold behind the cleaning mold;
(3) the crystal resin acrylic resin is added the dustless inserts polystyrene of nanometer;
(4) add batching such as curing agent epoxy curing agent, accelerant N-cyclohexyl-2-[4-morpholinodithio sulfenamide, antifoaming agent organosiloxane, transparent masterbatches polypropylene transparent masterbatch and stir well even; When resin adds batching; The attention ratio avoids causing cracking or sticking hand; Because of the hardening of resin time short, must be with disposable the using up of resin that adds batching, otherwise can't reuse;
(5) pour resin into mould after, shake mould, make in the mould resin flows even, mould is just put after evenly, prevent to be out of shape in the hardening of resin process;
(6) seed is put into resin.
(7) after resin must solidify fully, can slough mould, slough too early like mould, it is matt to cause staying on the product impression of the hand or product surface.
(8) with the product polishing, polishing can be painted if needed, makes the fragrant eupatorium herb standard sample, is embedded in the transparent resin 1 fragrant eupatorium herb in every block of resin through the fragrant eupatorium herb of confirming.
Embodiment 2
Standard sample uniformity and stability test that embodiment 1 makes:
(1) uniformity testing: uniformity testing is accomplished in two steps, and the one, " in the bottle " uniformity testing, the 2nd, " between bottle " uniformity testing.
1. " bottle in " uniformity testing: main consideration reduces the difference that exists between the fragrant eupatorium herb individuality as far as possible.Generally, " bottle in " uniformity testing will combine the expert to qualitatively judge to accomplish.100 of picked at random are carried out uniformity testing from the fragrant eupatorium herb behind the above-mentioned dusting cover.100 fragrant eupatorium herbs are divided into 5 groups, 20 every group, send 5 experts 5 groups of fragrant eupatorium herb seeds respectively and identify.Require the expert at first 20 fragrant eupatorium herbs to be carried out identification of morphology; Judge whether it is fragrant eupatorium herb; Provide the fragrant eupatorium herb identification mark then,, relatively whether there are differences between each individuality of fragrant eupatorium herb according to these identification marks; Whether the difference that exists can exert an influence to qualification result, provides uniformity conclusion between the fragrant eupatorium herb individuality.At last, the expert does not have doubt to the qualification result of 100 fragrant eupatorium herbs, and expert's conclusion is fragrant eupatorium herb.Each expert assert that all the nuance that exists between the fragrant eupatorium herb individuality can not exert an influence to this seed morphology qualification result, is uniform on form between the fragrant eupatorium herb individuality.
2. " between bottle " uniformity testing: mainly be the difference of considering between each standard sample individuality of packing back; After the checking fragrant eupatorium herb is mounted in the resin; Whether these outer packaging materials exert an influence to the external form of fragrant eupatorium herb, whether influence the identification of morphology of fragrant eupatorium herb.The fragrant eupatorium herb standard sample of external packing is accomplished and had to 20 of picked at random processing and fabricating; The external form of perusal fragrant eupatorium herb at first; And itself and the fragrant eupatorium herb of not packing normally processing compared, whether the fragrant eupatorium herb of confirming to be embedded in the resin externally there are differences on the form with the fragrant eupatorium herb of normally not packing processing.Secondly observe through anatomical lens, whether the fragrant eupatorium herb of confirming to be embedded in the resin there are differences with normal exposed fragrant eupatorium herb external form under anatomical lens, as there are differences and confirm whether these differences can exert an influence to the qualification result of fragrant eupatorium herb.Through 20 packaged fragrant eupatorium herb standard samples being carried out observation under naked eyes and the anatomical lens, externally on the form, the fragrant eupatorium herb that is embedded in the crystal does not almost have difference with normal exposed fragrant eupatorium herb.The identification of morphology result not influence of outer packaging material to fragrant eupatorium herb is described, fragrant eupatorium herb " between bottle " individuality also is uniform.
(2) stability test: the method for checking fragrant eupatorium herb stability needs with the naked eye or magnifying glass, anatomical lens are observed.Whether the morphological feature of observing different preservation conditions, difference " between bottle " fragrant eupatorium herb changes, and whether the variation of generation influences the result is identified.The design of preservation condition fully takes into account the various situation that standard sample possibly run in storage, transportation and use; Design under the different conditions such as illumination, temperature, wetness, vibration; Through certain time interval, all can not exert an influence to the form of fragrant eupatorium herb.Explain that this standard sample has good stability
The packing of fragrant eupatorium herb standard sample adopts can long preservation and the resin material wear-resistant, that light transmission is good, and makes through special processing technology.Fragrant eupatorium herb standard sample preservation condition: normal temperature is preserved down, and the sunlight direct projection is avoided in protection against the tide, the term of validity 5 years.
Claims (4)
1. the fragrant eupatorium herb standard sample is characterized in that: pass through the fragrant eupatorium herb of confirming and be embedded in the transparent resin 1 fragrant eupatorium herb in every block of resin.
2. the preparation method of fragrant eupatorium herb standard sample, it is characterized in that: concrete steps are:
First step standard sample source: the fragrant eupatorium herb seed that the field is gathered carries out isolation implant in growth in season after identifying, according to whole plant and flowers and fruits characteristic it is further verified, confirms the authenticity of fragrant eupatorium herb;
The second step standard sample expands numerous: at first disinfecting soil is handled; Be contained in the soil that disinfects in the big flowerpot then, neatly put flowerpot again and isolate in the solarium, all its growing state has been carried out observation and record in the whole growth season of fragrant eupatorium herb; After the fragrant eupatorium herb maturation; Gather and airing, the fragrant eupatorium herb that airing is good is contained in the drier to be preserved under the room temperature, prepares to be used for the screening and the making of standard sample;
The 3rd step standard sample screening: at first the fragrant eupatorium herb seed is carried out inactivation treatment; Be poured on through the fragrant eupatorium herb seed of inactivation treatment and carry out primary dcreening operation in the white disk; The seed that select full seed, is of moderate size; The seed picked out is prepared to carry out dusting cover, pay close attention to the nuance of the identification of morphology characteristic between the seed individuality, will pass through the seed that dusting cover picks out and be put in the white port grinding bottle subsequent use respectively;
The 4th step, standard sample preparation:
(1) makes mould, jaqan evenly is sprayed on die surface;
(2) install external mold behind the cleaning mold;
(3) crystal resin is added the dustless inserts of nanometer;
(4) add batching such as curing agent, promoter, antifoaming agent, transparent masterbatches and stir well even;
(5) pour resin into mould after, shake mould, make in the mould resin flows even, mould is just put after evenly, prevent to be out of shape in the hardening of resin process;
(6) seed is put into resin;
(7) after resin solidifies fully, slough mould;
(8) with product polishing, polishing.
3. the preparation method of fragrant eupatorium herb standard sample according to claim 1 is characterized in that: said laggard column criterion sample homogeneity of standard sample and the stability test of making:
(1) uniformity testing: the picked at random standard sample divides into groups, and identifies through the expert respectively, and the expert assert that the nuance that exists between individuality to not influence of fragrant eupatorium herb identification of morphology result, is uniform between individuality on form;
(2) stability test: choose the fragrant eupatorium herb standard sample that 20 packings complete immediately; Respectively through naked eyes or use magnifying glass, anatomical lens that its external form is observed; And sample that itself and packing are handled compared; The two does not externally have difference on the form, explains that this sample stability is good.
4. according to the preparation method of claim 2 or 3 described fragrant eupatorium herb standard samples; It is characterized in that: said laggard column criterion sample homogeneity of standard sample and the stability test of making: inactivation treatment is in said the 3rd step standard sample screening: seed is placed in 50 ℃ the insulating box; Carry out seed germination experiment behind the 48h, confirm that seed loses vigor.
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| CN201110220784.5A CN102415369B (en) | 2011-08-03 | 2011-08-03 | Eupatorium odoratum standard sample and preparation method thereof |
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| CN201110220784.5A CN102415369B (en) | 2011-08-03 | 2011-08-03 | Eupatorium odoratum standard sample and preparation method thereof |
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| CN102415369A true CN102415369A (en) | 2012-04-18 |
| CN102415369B CN102415369B (en) | 2014-07-30 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835390A (en) * | 2012-08-23 | 2012-12-26 | 黑龙江东方学院 | Preparation method of detachable plant three-dimensional specimen |
| CN105842025A (en) * | 2016-03-22 | 2016-08-10 | 王有福 | Yellow soybean imperfect granule standard sample preparation method |
| CN105842024A (en) * | 2016-03-22 | 2016-08-10 | 王有福 | Preparation method for wild soybean standard sample |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4588579A (en) * | 1982-10-19 | 1986-05-13 | Rolf Bachhuber | Process for the production of thin sections of biological tissue |
| CN101341868A (en) * | 2008-08-20 | 2009-01-14 | 杜静磊 | Process for preparing biological specimen |
| CN101653113A (en) * | 2009-09-10 | 2010-02-24 | 边望之 | Method for manufacturing nontoxic specimen of marine organism |
| CN102134398A (en) * | 2010-12-17 | 2011-07-27 | 滨州医学院 | Environment-friendly biological plasticizer for biological specimen preservation |
-
2011
- 2011-08-03 CN CN201110220784.5A patent/CN102415369B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4588579A (en) * | 1982-10-19 | 1986-05-13 | Rolf Bachhuber | Process for the production of thin sections of biological tissue |
| CN101341868A (en) * | 2008-08-20 | 2009-01-14 | 杜静磊 | Process for preparing biological specimen |
| CN101653113A (en) * | 2009-09-10 | 2010-02-24 | 边望之 | Method for manufacturing nontoxic specimen of marine organism |
| CN102134398A (en) * | 2010-12-17 | 2011-07-27 | 滨州医学院 | Environment-friendly biological plasticizer for biological specimen preservation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835390A (en) * | 2012-08-23 | 2012-12-26 | 黑龙江东方学院 | Preparation method of detachable plant three-dimensional specimen |
| CN105842025A (en) * | 2016-03-22 | 2016-08-10 | 王有福 | Yellow soybean imperfect granule standard sample preparation method |
| CN105842024A (en) * | 2016-03-22 | 2016-08-10 | 王有福 | Preparation method for wild soybean standard sample |
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| Publication number | Publication date |
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| CN102415369B (en) | 2014-07-30 |
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Inventor after: Liu Huiqiu Inventor after: Wang Youfu Inventor after: Cao Dongmei Inventor after: Li Xin Inventor before: Liu Huiqiu Inventor before: Wang Youfu Inventor before: Jiang Li Inventor before: Xu Fengmin |
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