CN102134398A - Environment-friendly biological plasticizer for biological specimen preservation - Google Patents
Environment-friendly biological plasticizer for biological specimen preservation Download PDFInfo
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- CN102134398A CN102134398A CN201010612866XA CN201010612866A CN102134398A CN 102134398 A CN102134398 A CN 102134398A CN 201010612866X A CN201010612866X A CN 201010612866XA CN 201010612866 A CN201010612866 A CN 201010612866A CN 102134398 A CN102134398 A CN 102134398A
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Abstract
The invention discloses an environment-friendly biological plasticizer for biological specimen preservation, which is prepared from the following components in parts by weight: 100 parts of silicone resin, 20-100 parts of plasticizer, 0.5-5 parts of catalyst and 2-10 parts of crosslinking and curing agent, wherein the silicone resin is hydroxyl-terminated methyl silicone oil, the plasticizer is methyl-terminated methyl silicone oil, the catalyst is organotin, and the crosslinking and curing agent is organosiloxane. The invention has the advantages of no toxicity, no harm, no smell and no pollution, and is transparent; the specimen plasticized by the plasticizer has the advantages of strong authenticity, good hand feeling and high vividness, and can be stored for a long time; the environment-friendly biological plasticizer for biological specimen preservation can ensure the manufacture of biological specimens to be carried out at ordinary temperature, and avoids using chloro-platinic acid and other noble and highly-corrosive reagents, thereby greatly lowering the cost and reducing the harm to operating personnel; and the invention avoids using a diluter, and simplifies the operation steps.
Description
Technical field:
The present invention relates to a kind of environment protection biological fluidizer that biological sample is preserved that is used for, belong to the fluidizer technical field.
Background technology:
At present, the preservation that is used for biological sample mainly is that formaldehyde (formalin) soaks, or the plasticizing of biological sample plasticized silica gel, and formaldehyde is poisonous, flavor is arranged, and is bigger to the human injury, contaminate environment.Make biological plasticized sample with silica gel, making processes is loaded down with trivial details, agents useful for same contains materials such as volatile organic solvent and Platinic chloride, diminishes operator's health during concrete operations.Simultaneously also there are problems such as shelf time weak point, easy deformation, cost of manufacture be high in they.
Summary of the invention:
The objective of the invention is to overcome the deficiency of above-mentioned prior art and provide a kind of nontoxic, pollution-free, cost is low, avoids the injury to operator, it is strong to make the sample verity, good hand touch, the shelf time is long, and operation steps simply is used for the biological plasticizing agent that biological sample is preserved.
Purpose of the present invention can reach by following measure: a kind of environment protection biological fluidizer that is used for the biological sample preservation, it is characterized in that being made up of silicone resin, softening agent, catalyzer and crosslinking and curing agent, their weight proportion is a silicone resin: softening agent: catalyzer: crosslinking and curing agent=100: 20～100: 05～5: 2～10.
In order further to realize purpose of the present invention, described silicone resin is hydroxy-end capped methyl-silicone oil, and its chemical structural formula is as follows:
N=50～600, molecular weight are 5000～40000.
In order further to realize purpose of the present invention, described softening agent is the methyl-silicone oil of methyl blocking, and its chemical structural formula is as follows:
N=5～600, molecular weight are 500～40000.
In order further to realize purpose of the present invention, described catalyzer is an organotin, and its chemical structural formula is as follows:
R in the formula
1Be C
2H
5～C
8H
17In a kind of, R
2Be C
6H
13～C
12H
25In a kind of.
In order further to realize purpose of the present invention, described crosslinking and curing agent is an organo-siloxane, and its chemical structural formula is as follows:
R wherein
3Be CH
3～C
2H
5In a kind of, R
4Be CH
3～C
2H
5Or NH
2CH
2CH
2CH
2In a kind of.
The present invention can produce following positively effect compared with the prior art: silicone resin of the present invention adopts hydroxy-end capped methyl-silicone oil, and softening agent adopts the methyl-silicone oil of methyl blocking, and the methyl-silicone oil of small molecular weight plays plastification, makes the soft high resilience of sample.Catalyzer adopts organotin, can accelerate solidification rate, improves sample stability.Crosslinking and curing agent is an organo-siloxane, has that volatility is good, a fast setting, characteristic such as easy to use.The present invention has following advantage in sum:
1, nontoxic, harmless, tasteless, pollution-free, transparent;
2, the sample verity is strong, good hand touch;
3, guaranteeing that biological sample is made can carry out at normal temperatures, and cost reduces significantly;
4, sample can prolonged preservation after plastifying, and form is life-like;
5, avoid using valuable, deep-etching row reagent such as Platinic chloride, reduce cost greatly and operator's injury;
6, avoid using thinner, simplified operation steps.
Embodiment: following the specific embodiment of the present invention is elaborated:
Raw material is prepared:
Silicone resin: adopt hydroxy-end capped methyl-silicone oil, its chemical structural formula is as follows:
N=50～600, molecular weight are 5000～40000.
Softening agent: adopt the methyl-silicone oil of methyl blocking, its chemical structural formula is as follows:
N=5～600, molecular weight are 500～40000.
Catalyzer: adopt organotin, its chemical structural formula is as follows:
R in the formula
1Be C
2H
5～C
8H
17In a kind of, R
2Be C
6H
13～C
12H
25In a kind of.
Crosslinking and curing agent: adopt organo-siloxane, its chemical structural formula is as follows:
R wherein
3Be CH
3～C
2H
5In a kind of, R
4Be CH
3～C
2H
5Or NH
2CH
2CH
2CH
2In a kind of.
Embodiment 1: 2 kilograms of the methyl-silicone oils (present embodiment n=5～50, molecular weight are 500～5000), organotin (the present embodiment R that get 2 kilograms of hydroxy-end capped methyl-silicone oils (present embodiment n=50～600, molecular weight are 5000～40000), methyl blocking
1Be C
2H
5, R
2Be C
6H
13, it is diethyl two caproic acid tin) and 0.1 kilogram and 0.2 kilogram of (present embodiment R of organo-siloxane
3Be CH
3, R
4Be CH
3, it is a tetramethoxy-silicane) and the composition fluidizer.
Embodiment 2: 0.4 kilogram of the methyl-silicone oil (present embodiment n=5100, molecular weight are 500～10000), organotin (the present embodiment R that get 2 kilograms of hydroxy-end capped methyl-silicone oils (present embodiment n=50～600, molecular weight are 500～40000), methyl blocking
1Be C
8H
17, R
2Be C
12H
25, it is dioctyl two lauric acid tin) and 0.01 kilogram and 0.04 kilogram of (present embodiment R of organo-siloxane
3Be C
2H
5, R
4Be C
2H
5, it is a tetraethoxysilane) and the composition fluidizer.
Embodiment 3: 1 kilogram of the methyl-silicone oil (present embodiment n=5～600, molecular weight are 500～40000), organotin (the present embodiment R that get 2 kilograms of hydroxy-end capped methyl-silicone oils (present embodiment n=50～600, molecular weight are 5000～40000), methyl blocking
1Be C
4H
9, R
2Be C
8H
17, it is dibutyl two stannous octoates) and 0.04 kilogram and 0.1 kilogram of (present embodiment R of organo-siloxane
3Be C
2H
5, R
4Be C
2H
5, tetraethoxysilane) and form into fluidizer.
Embodiment 4: get hydroxy-end capped methyl-silicone oil (present embodiment n=50～600, molecular weight is 5000～40000) 2 kilograms, 0.8 kilogram of the methyl-silicone oil (present embodiment n=50～600, molecular weight are 5000～40000) of methyl blocking, organotin (present embodiment R
1Be C
6H
13, R
2Be C
8H
17, it is dihexyl two stannous octoates) and 0.02 kilogram and 0.05 kilogram of (present embodiment R of organo-siloxane
3Be C
2H
5, R
4Be NH
2CH
2CH
2CH
2, it is amino propoxy-triethoxyl silane) and the composition fluidizer.
During use, the above-mentioned hydroxy-end capped methyl-silicone oil and the methyl-silicone oil of methyl blocking are mixed, back adding organotin mixes makes mixed solution.To impregnated in through the biological sample after fixing and dehydration, the degreasing in the above-mentioned mixed solution under the normal temperature and place 2-7 days, and biological sample be impregnated in the negative pressure of vacuum refrigerator-freezer that is placed with mixed solution then and replace.After treating displacement fully, carry out the high bake of biological sample.To put into airtight container through the biological sample after the baking box baking, temperature is controlled at 30 ℃～50 ℃.Place the organo-siloxane that holds with beaker in the airtight container, make the organo-siloxane atomizing with membranelle pump (little air pump), be full of in the whole airtight container, make biological sample continue to be exposed in the gas of this crosslinking and curing agent and carry out polymerization, sclerosis in 3-7 days is finished.
Above embodiment is described preferred implementation of the present invention; be not that scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that the common engineering technical personnel in this area make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (5)
1. one kind is used for the environment protection biological fluidizer that biological sample is preserved, it is characterized in that being made up of silicone resin, softening agent, catalyzer and crosslinking and curing agent, their weight proportion is a silicone resin: softening agent: catalyzer: crosslinking and curing agent=100: 20～100: 0.5～5: 2～10.
2. a kind of environment protection biological fluidizer that biological sample is preserved that is used for according to claim 1 is characterized in that described silicone resin is hydroxy-end capped methyl-silicone oil, and its chemical structural formula is as follows:
N=50～600, molecular weight are 5000～40000.
3. a kind of environment protection biological fluidizer that biological sample is preserved that is used for according to claim 1 is characterized in that described softening agent is the methyl-silicone oil of methyl blocking, and its chemical structural formula is as follows:
N=5～600, molecular weight are 500～40000.
4. a kind of environment protection biological fluidizer that biological sample is preserved that is used for according to claim 1 is characterized in that described catalyzer is an organotin, and its chemical structural formula is as follows:
R in the formula
1Be C
2H
5～C
8H
17In a kind of, R
2Be C
6H
13～C
12H
25In a kind of.
5. a kind of environment protection biological fluidizer that biological sample is preserved that is used for according to claim 1 is characterized in that described crosslinking and curing agent is an organo-siloxane, and its chemical structural formula is as follows:
R wherein
3Be CH
3～C
2H
5In a kind of, R
4Be CH
3～C
2H
5Or NH
2CH
2CH
2CH
2In a kind of.
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|---|---|---|---|
| CN201010612866XA CN102134398B (en) | 2010-12-17 | 2010-12-17 | Environment-friendly biological plasticizer for biological specimen preservation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010612866XA CN102134398B (en) | 2010-12-17 | 2010-12-17 | Environment-friendly biological plasticizer for biological specimen preservation |
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| CN102134398A true CN102134398A (en) | 2011-07-27 |
| CN102134398B CN102134398B (en) | 2012-08-22 |
Family
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|---|---|---|---|
| CN201010612866XA Active CN102134398B (en) | 2010-12-17 | 2010-12-17 | Environment-friendly biological plasticizer for biological specimen preservation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415369A (en) * | 2011-08-03 | 2012-04-18 | 刘卉秋 | Eupatorium odoratum standard sample and preparation method thereof |
| CN108990968A (en) * | 2018-09-25 | 2018-12-14 | 河南科技大学 | A kind of production method of the biological plasticized sample of Alfalfa primary colors overall picture |
| CN111066774A (en) * | 2019-12-31 | 2020-04-28 | 郑州国之正生物科技有限公司 | Method for manufacturing plasticized specimen |
| CN111742920A (en) * | 2020-07-07 | 2020-10-09 | 西南林业大学 | Compound liquid for preparing edible fungus specimen and preparation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009144192A1 (en) * | 2008-05-26 | 2009-12-03 | Bluestar Silicones France Sas | Pressure-sensitive adhesive silicone composition |
| CN101775217A (en) * | 2009-12-30 | 2010-07-14 | 苏州天山新材料技术有限公司 | Room-temperature vulcanized organic polyorganosiloxane composition and preparation method |
| CN101851486A (en) * | 2010-06-04 | 2010-10-06 | 江苏天辰硅材料有限公司 | Two-component solar sealant and preparation method thereof |
-
2010
- 2010-12-17 CN CN201010612866XA patent/CN102134398B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009144192A1 (en) * | 2008-05-26 | 2009-12-03 | Bluestar Silicones France Sas | Pressure-sensitive adhesive silicone composition |
| CN101775217A (en) * | 2009-12-30 | 2010-07-14 | 苏州天山新材料技术有限公司 | Room-temperature vulcanized organic polyorganosiloxane composition and preparation method |
| CN101851486A (en) * | 2010-06-04 | 2010-10-06 | 江苏天辰硅材料有限公司 | Two-component solar sealant and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415369A (en) * | 2011-08-03 | 2012-04-18 | 刘卉秋 | Eupatorium odoratum standard sample and preparation method thereof |
| CN108990968A (en) * | 2018-09-25 | 2018-12-14 | 河南科技大学 | A kind of production method of the biological plasticized sample of Alfalfa primary colors overall picture |
| CN111066774A (en) * | 2019-12-31 | 2020-04-28 | 郑州国之正生物科技有限公司 | Method for manufacturing plasticized specimen |
| CN111742920A (en) * | 2020-07-07 | 2020-10-09 | 西南林业大学 | Compound liquid for preparing edible fungus specimen and preparation method |
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| Publication number | Publication date |
|---|---|
| CN102134398B (en) | 2012-08-22 |
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Inventor after: Huang Fei Inventor after: Zhang Chunlei Inventor after: Zhang Luping Inventor after: Zhou Shuai Inventor before: Huang Fei Inventor before: Zhang Chunlei Inventor before: Zhang Luping Inventor before: Zhou Shuai |
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Effective date of registration: 20220908 Address after: 276100 floor 2-3, building 11, zone a, high tech electronic industrial park, Tancheng County, Linyi City, Shandong Province Patentee after: SHANDONG JUZHONG DIGITAL MEDICAL TECHNOLOGY DEVELOPMENT Co.,Ltd. Address before: 346 Guanhai Road, Laishan District, Yantai City, Shandong Province Patentee before: BINZHOU MEDICAL University |
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