CN1233391A - Preservation liquid for animal body specimen - Google Patents
Preservation liquid for animal body specimen Download PDFInfo
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- CN1233391A CN1233391A CN 98108129 CN98108129A CN1233391A CN 1233391 A CN1233391 A CN 1233391A CN 98108129 CN98108129 CN 98108129 CN 98108129 A CN98108129 A CN 98108129A CN 1233391 A CN1233391 A CN 1233391A
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Abstract
The present invention relates to a preserving liguid for animal specimen, in particular, it is an aqueous base animal specimen preserving liquid. The invented preserving liquid is composed of at least one sodium salt or potassium salt preservative and at least one polyhydroxy colour retention agent and water, in which the total mole ratio and preservative and colour retention agent is 1:1 to 1:50, the tolal mole concentration of preservative in the preserving liquid is 0.010-1.0 mole liter solution. The preserving liquid contains no alcobol and formaldehyde, and has no toxic and side effect to human body and environment.
Description
The present invention relates to a kind of preservation liquid for animal body specimen, particularly a kind of water base preservation liquid for animal body specimen.
As everyone knows, sample is kept in the liquid, sample is kept intact as far as possible, help viewing and admiring of scientific research, teaching and people etc.Preserve sample up to now both at home and abroad, most often utilize formalin.As U.S. Pat 5,350,670 disclose a kind of composition of preserving animal or plant specimen, and said composition is mixed into particle by boric acid, sodium chloride and phenol, form with formalin and the volatile alcohols allotment of tool again.But formalin has the strong impulse smell, in the process of making and using, contaminated environment not only, the more important thing is and be detrimental to health, autopsy teaching as medical college, even faculty's band muzzles, its eyes and respiratory system still are subjected to harm in various degree, have become for many years to perplex a difficult problem of medical college's teaching; Formaldehyde preserve liquid easily make the sample body harden, fade, not fresh-keeping; Formalin is easily muddy in addition, needs often to change.Therefore people wish to find the preservation liquid for animal body specimen that does not contain formaldehyde.
Ethanol medically can be used as disinfectant and bactericide, is subjected to the inspiration of this traditional method, develops in recent years with the preservation liquid for animal body specimen of ethanol as active ingredient.Disclose a kind of biological sample as Chinese patent CN88105221A and protected the look preservative, it is that materials such as ethanol, potassium acetate, sodium sulphate, glycerine, water are mixed with mixed liquor in proportion; Chinese patent CN1086658A discloses a kind of antiseptic preserving liquid for dead body specimen, and this preservation liquid is formulated by benzoate, potassium nitrate, ethanol, glycerine.But the long-term use of preservation liquid that contains ethanol can make animal protein hardening distortion, and this may be because the molecular weight of ethanol is less, and long-term use can be penetrated into due to the sample inside.
The inventor is surprised to find that: can make the preservation liquid for animal body specimen that does not contain ethanol by selecting suitable component and proportioning thereof, not only can overcome the above-mentioned shortcoming that ethanol causes, and have better preservation effect.
The object of the present invention is to provide a kind of preservation liquid for animal body specimen that does not contain formaldehyde or ethanol.
The preservation liquid for animal body specimen that provides a kind of preservation effect good is provided.
To achieve the above object of the invention, preservation liquid for animal body specimen of the present invention is made up of the preservative of at least a sodium salt or sylvite class, at least a polyhydroxy class color preserving agent and water, total mol ratio of wherein used preservative and color preserving agent is 1: 1～1: 50, be 1: 1～1: 36 preferably, the total mol concentration of preservative is 0.010～1.0 mol solution in described preservation liquid, is 0.051～0.73 mol solution preferably.
The selected preservative of the present invention is sodium salt or sylvite class preservative, is reductant preferably, and because of effectively absorbing the micro amount of oxygen of preserving in the liquid, and its oxidation product does not have color, thereby can guarantee to preserve liquid nondiscolouring, without putrefaction for a long time, never degenerate.Preservative of the present invention can be selected from one or more in sodium chloride, potassium chloride, natrium nitrosum, potassium nitrite, Sodium Benzoate, Potassium Benzoate, benzene sulfonic acid sodium salt, benzene sulfonic acid potassium, sodium sulphite, potassium sulfite, sodium hydrogensulfite, potassium bisulfite, sodium dithionite, potassium hyposulfite, sodium tetraborate, dipotassium tetraborate, potassium tartrate, sodium tartrate, potassium sorbate, sodium sorbate, potassium malate or the natrium malicum.
Because the zoological specimens body is rich in moisture and polar substances, so polyhydroxy class color preserving agent can carry out the effective chemical bonding with it and form hydrogen bond, thereby body reached protect Semu.Color preserving agent of the present invention can be selected from one or more in ethylene glycol, glycerine, diethylene glycol, triethylene glycol, pentaerythrite, polyvinyl alcohol, carboxymethyl cellulose, carboxyethyl cellulose, vitamin C, alkali modification glucose or the fructose.
At least a preservative, at least a color preserving agent and water are mixed with mixed solution according to the above ratio, promptly make preservation liquid for animal body specimen of the present invention.
Preservation liquid for animal body specimen of the present invention has that preparation is simple, raw material is easy to get, to human body and environment has no side effect and serviceability temperature advantages such as (can use in-40 ℃～40 ℃ scopes) in extensive range, can be widely used in the preservation of zoological specimens.
The present invention is further illustrated below in conjunction with embodiment, but these embodiment do not limit protection scope of the present invention.Embodiment 1
Ethylene glycol 20ml, sodium chloride 10g, Sodium Benzoate 15g and vitamin C 10g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available raw pork directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 2
Ethylene glycol 50ml, benzene sulfonic acid sodium salt 10g, natrium nitrosum 15g and glycerine 10ml are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available raw pork directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 3
Ethylene glycol 50m1, glycerine 35ml, benzene sulfonic acid sodium salt 5g and borax saturated solution 10ml are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500m1.A living pigeon after dissecting directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 4
Ethylene glycol 50ml, fructose 15g, natrium malicum 5g and sodium chloride 3g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available raw pork directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 5
Ethylene glycol 50ml, benzene sulfonic acid sodium salt 15g and alkali modification glucose 15g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available raw pork directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 6
Ethylene glycol 50ml, Sodium Benzoate 5g, sodium chloride 3g and borax saturated solution 10ml are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.A living pigeon after dissecting directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 7
Ethylene glycol 50ml, diethylene glycol 20ml, Sodium Benzoate 4g and natrium nitrosum 3g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available raw pork directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 8
Ethylene glycol 30ml, polyvinyl alcohol 10g, Sodium Benzoate 20g and natrium nitrosum 10g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Living hairtail directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 9
Ethylene glycol 20ml, triethylene glycol 30ml, natrium nitrosum 10g and potassium tartrate 15g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Living frog after dissecting directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 10
Carboxymethyl cellulose 5g, pentaerythrite 40ml, potassium sorbate 15g and sodium dithionite 5g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available living mutton directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 11
Ethylene glycol 20ml, potassium chloride 10g, Sodium Benzoate 15g and vitamin C 10g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available raw pork directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 12
Ethylene glycol 50ml, glycerine 35ml, benzene sulfonic acid potassium 5g and borax saturated solution 10ml are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.A living pigeon after dissecting directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 13
Ethylene glycol 30ml, polyvinyl alcohol 10g, Potassium Benzoate 20g and potassium nitrite 10g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Living hairtail directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 14
Ethylene glycol 50ml, Sodium Benzoate 5g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available living beef directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Embodiment 15
Glycerine 50ml, Sodium Benzoate 5g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available living beef directly is kept in the specimen bottle with cover with preservation liquid.After 2 years, sample color and luster, shape all do not have change.Comparative Examples 1
Glycerine 25ml, ethanol 25ml and Sodium Benzoate 5g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available living beef directly is kept in the specimen bottle with cover with preservation liquid.After six months, the sample body is obvious atrophy shape and hardness obviously increases.Comparative Examples 2
Ethylene glycol 25ml, ethanol 25ml and Sodium Benzoate 5g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available living beef directly is kept in the specimen bottle with cover with preservation liquid.After six months, the sample body is obvious atrophy shape and hardness obviously increases.Comparative Examples 3
Glycerine 25ml, ethylene glycol 15ml, ethanol 10ml and Sodium Benzoate 5g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available living beef directly is kept in the specimen bottle with cover with preservation liquid.After six months, the sample body is obvious atrophy shape and hardness obviously increases.Comparative Examples 4
Glycerine 15ml, ethylene glycol 20ml, ethanol 15ml and Sodium Benzoate 5g are mixed, and thin up promptly gets preservation liquid for animal body specimen to 500ml.Commercially available living beef directly is kept in the specimen bottle with cover with preservation liquid.After six months, the sample body is obvious atrophy shape and hardness obviously increases.
By the data of Comparative Examples and embodiment as can be seen: after preserving biological sample with the preservation liquid that contains ethanol, sample just deforms at short notice, and experiment also to find to contain the ratio of ethanol high more, the place of withering of sample is just obvious more.And with of the present invention do not contain ethanol exist liquid to preserve biological sample after, sample is also kept intact in for a long time.
Claims (9)
1. a preservation liquid for animal body specimen is made up of the preservative of at least a sodium salt or sylvite class, at least a polyhydroxy class color preserving agent and water, total mol ratio of wherein used preservative and color preserving agent is 1: 1～1: 50, and the total mol concentration of preserving preservative in the liquid is 0.010～1.0 mol solution.
2. preservation liquid according to claim 1, the total mol ratio that it is characterized in that used preservative and color preserving agent is 1: 1～1: 36.
3. preservation liquid according to claim 1 and 2, the total mol concentration that it is characterized in that preserving preservative in the liquid are 0.051～0.73 mol solution.
4. preservation liquid according to claim 1 and 2 is characterized in that described preservative is selected from one or more in sodium chloride, potassium chloride, natrium nitrosum, potassium nitrite, Sodium Benzoate, Potassium Benzoate, benzene sulfonic acid sodium salt, benzene sulfonic acid potassium, sodium sulphite, potassium sulfite, sodium hydrogensulfite, potassium bisulfite, sodium dithionite, potassium hyposulfite, sodium tetraborate, dipotassium tetraborate, potassium tartrate, sodium tartrate, potassium sorbate, sodium sorbate, potassium malate or the natrium malicum.
5. preservation liquid according to claim 3 is characterized in that described preservative is selected from one or more in sodium chloride, potassium chloride, natrium nitrosum, potassium nitrite, Sodium Benzoate, Potassium Benzoate, benzene sulfonic acid sodium salt, benzene sulfonic acid potassium, sodium sulphite, potassium sulfite, sodium hydrogensulfite, potassium bisulfite, sodium dithionite, potassium hyposulfite, sodium tetraborate, dipotassium tetraborate, potassium tartrate, sodium tartrate, potassium sorbate, sodium sorbate, potassium malate or the natrium malicum.
6. preservation liquid according to claim 1 and 2 is characterized in that described color preserving agent is selected from one or more in ethylene glycol, glycerine, diethylene glycol, triethylene glycol, pentaerythrite, polyvinyl alcohol, carboxymethyl cellulose, carboxyethyl cellulose, vitamin C, alkali modification glucose or the fructose.
7. preservation liquid according to claim 3 is characterized in that described color preserving agent is selected from one or more in ethylene glycol, glycerine, diethylene glycol, triethylene glycol, pentaerythrite, polyvinyl alcohol, carboxymethyl cellulose, carboxyethyl cellulose, vitamin C, alkali modification glucose or the fructose.
8. preservation liquid according to claim 1 and 2 can be used as preservation liquid for animal body specimen.
9. can be used as preservation liquid for animal body specimen according to preservation liquid according to claim 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98108129 CN1131667C (en) | 1998-04-30 | 1998-04-30 | Preservation liquid for animal body specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98108129 CN1131667C (en) | 1998-04-30 | 1998-04-30 | Preservation liquid for animal body specimen |
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| CN1233391A true CN1233391A (en) | 1999-11-03 |
| CN1131667C CN1131667C (en) | 2003-12-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 98108129 Expired - Fee Related CN1131667C (en) | 1998-04-30 | 1998-04-30 | Preservation liquid for animal body specimen |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100448351C (en) * | 2007-07-12 | 2009-01-07 | 江苏省原子医学研究所 | Specimen anti-corrosion stationary liquid |
| CN102578073A (en) * | 2012-02-07 | 2012-07-18 | 王永红 | Preserving fluid for biological sample |
| CN104365581A (en) * | 2014-11-05 | 2015-02-25 | 中山大学 | Novel specimen conservation solution |
| CN104542571A (en) * | 2015-01-24 | 2015-04-29 | 中南大学 | Novel cadaver specimen preserving fluid |
| CN105052891A (en) * | 2015-07-16 | 2015-11-18 | 内蒙古科技大学包头医学院 | Long-stem storage method for anatomical visceral specimen |
| CN105432661A (en) * | 2015-07-08 | 2016-03-30 | 清华大学 | Waste drosophila treatment liquid |
| CN106257567A (en) * | 2016-03-11 | 2016-12-28 | 宁夏大学 | Lumbricus section and manufacture method and application thereof for microsurgery training |
| CN107509722A (en) * | 2017-08-30 | 2017-12-26 | 迈克生物股份有限公司 | Sample preservation liquid |
| CN108244093A (en) * | 2018-04-10 | 2018-07-06 | 大连理工大学 | A kind of insect specimen color preserving agent, preparation method and color-retention method |
| CN108931408A (en) * | 2018-06-15 | 2018-12-04 | 山东聚众数字医学科技开发有限公司 | A kind of anatomic teaching sample guarantor's color fixing liquid and preparation method thereof |
| CN109169634A (en) * | 2018-10-11 | 2019-01-11 | 内蒙古科技大学包头医学院 | The preservation liquid of human anatomy internal organ sample |
| CN109566599A (en) * | 2018-12-25 | 2019-04-05 | 南京健邦锦源医疗仪器有限公司 | A kind of exfoliated cell preservative fluid |
| CN110024774A (en) * | 2018-01-12 | 2019-07-19 | 安庆师范大学 | A kind of production method of pigeon skinned-specimen |
| CN110720452A (en) * | 2019-11-05 | 2020-01-24 | 南通大学 | Method for optimizing preservation of pathological gross specimens |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
| CN112695069A (en) * | 2020-12-22 | 2021-04-23 | 浙江海洋大学 | Application and preparation method of Tris-HCl solution |
-
1998
- 1998-04-30 CN CN 98108129 patent/CN1131667C/en not_active Expired - Fee Related
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100448351C (en) * | 2007-07-12 | 2009-01-07 | 江苏省原子医学研究所 | Specimen anti-corrosion stationary liquid |
| CN102578073A (en) * | 2012-02-07 | 2012-07-18 | 王永红 | Preserving fluid for biological sample |
| CN104365581A (en) * | 2014-11-05 | 2015-02-25 | 中山大学 | Novel specimen conservation solution |
| CN104365581B (en) * | 2014-11-05 | 2016-08-24 | 中山大学 | A kind of Novel specimen preserves liquid |
| CN104542571A (en) * | 2015-01-24 | 2015-04-29 | 中南大学 | Novel cadaver specimen preserving fluid |
| CN104542571B (en) * | 2015-01-24 | 2016-05-18 | 中南大学 | A kind of preserving corpse samples liquid |
| CN105432661A (en) * | 2015-07-08 | 2016-03-30 | 清华大学 | Waste drosophila treatment liquid |
| CN105052891A (en) * | 2015-07-16 | 2015-11-18 | 内蒙古科技大学包头医学院 | Long-stem storage method for anatomical visceral specimen |
| CN105052891B (en) * | 2015-07-16 | 2017-03-08 | 内蒙古科技大学包头医学院 | The long-term preservation method of anatomy internal organ sample |
| CN106257567B (en) * | 2016-03-11 | 2020-04-10 | 宁夏大学 | Earthworm section for microsurgery training, and manufacturing method and application thereof |
| CN106257567A (en) * | 2016-03-11 | 2016-12-28 | 宁夏大学 | Lumbricus section and manufacture method and application thereof for microsurgery training |
| CN107509722A (en) * | 2017-08-30 | 2017-12-26 | 迈克生物股份有限公司 | Sample preservation liquid |
| CN107509722B (en) * | 2017-08-30 | 2020-11-10 | 迈克生物股份有限公司 | Specimen preservation solution |
| CN110024774A (en) * | 2018-01-12 | 2019-07-19 | 安庆师范大学 | A kind of production method of pigeon skinned-specimen |
| CN108244093A (en) * | 2018-04-10 | 2018-07-06 | 大连理工大学 | A kind of insect specimen color preserving agent, preparation method and color-retention method |
| CN108931408A (en) * | 2018-06-15 | 2018-12-04 | 山东聚众数字医学科技开发有限公司 | A kind of anatomic teaching sample guarantor's color fixing liquid and preparation method thereof |
| CN108931408B (en) * | 2018-06-15 | 2021-08-27 | 山东聚众数字医学科技开发有限公司 | Color-preserving fixing liquid for dissecting teaching specimen and preparation method thereof |
| CN109169634A (en) * | 2018-10-11 | 2019-01-11 | 内蒙古科技大学包头医学院 | The preservation liquid of human anatomy internal organ sample |
| CN109566599A (en) * | 2018-12-25 | 2019-04-05 | 南京健邦锦源医疗仪器有限公司 | A kind of exfoliated cell preservative fluid |
| CN110720452A (en) * | 2019-11-05 | 2020-01-24 | 南通大学 | Method for optimizing preservation of pathological gross specimens |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
| CN112695069A (en) * | 2020-12-22 | 2021-04-23 | 浙江海洋大学 | Application and preparation method of Tris-HCl solution |
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| Publication number | Publication date |
|---|---|
| CN1131667C (en) | 2003-12-24 |
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