CN106973888A - A kind of preservation liquid of pathological tissue specimen - Google Patents
A kind of preservation liquid of pathological tissue specimen Download PDFInfo
- Publication number
- CN106973888A CN106973888A CN201710196330.6A CN201710196330A CN106973888A CN 106973888 A CN106973888 A CN 106973888A CN 201710196330 A CN201710196330 A CN 201710196330A CN 106973888 A CN106973888 A CN 106973888A
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- China
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- parts
- pathological tissue
- glutaraldehyde
- preservation liquid
- tissue specimen
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
Abstract
The invention discloses a kind of preservation liquid of pathological tissue specimen, it is mixed by weight ratio by following raw material：25 parts of potassium sorbate, 10 20 parts of industrial alcohol, 13 parts of glycerine, 23 parts of glutaraldehyde, 8.5 9.5 parts of sodium chloride, 0.6 1 parts of Nano Silver, 10 20 parts of citric acid, 60 100 parts of water.Specimen preserving liquid of the present invention is the liquid of water white transparency, is had no irritating odor, fungus and mildew resistance excellent performance；The tissue morphology and color and luster of protozoa sample can be effectively kept, paint having no volatile substances, effect persistently, can be effectively reduced cost；And without poisonous and harmful substance, environmental protection mitigates the burden of environment.
Description
Technical field
The present invention relates to pathological tissue Techniques of preserving field, the preservation liquid of specifically a kind of pathological tissue specimen.
Background technology
From operating room to pathology department, clinical laboratory's surgery, tissue biopsy, histopathology section office, gastroscope section office etc. are needed to group
Knit or cell etc. fixation, store, keep sample, be required for using specimen preserving liquid, existing preservation liquid is mainly formalin guarantor
Liquid storage.Formalin specimen preserving liquid, there is good fixation, this be due to formaldehyde and sample protein functional group it is big
Amount is combined, and long-term placement further polymerization forms paraformaldehyde.This process is allowed to form appropriateness in protein peptide interchain
Crosslinking, can sterilize, the tissue morphology of sample can be kept again.But, formaldehyde, benzene class are defined as causing by the World Health Organization
Cancer material, is listed in high poison chemicals, meeting intense stimulus eyes, skin, respiratory mucosa etc., ultimately causes immunologic function different
Often, hepatic injury and central nervous system are affected, will cause fetal anomaly, leukaemia, chronic respiratory disease, nasopharyngeal carcinoma,
The diseases such as feminine menstrual disorder, acute confusion depression, serious threat is caused to health.Simultaneously because formalin
Low boiling point, therefore the highly volatile in room temperature, accelerate with the rising evaporation rate of temperature, give out strong impulse smell,
Severe contamination laboratory environment.Over time, air formaldehyde concentration is also constantly raised, to medical science, biology and dynamic
The health of thing worker causes serious threat.In addition, the sample preserved for a long time through formalin, it may occur that go mouldy, xanthochromia,
Sample such as is hardened the phenomenon.
The content of the invention：
It is an object of the present invention to provide the preservation liquid of pathological tissue specimen a kind of.The preservation liquid is free of formaldehyde and Fu Er
Malin, volatilization is slow, has no irritating odor, toxicity is very low, safe, will not pollute experimental situation, preservation effect is good, when longer
Between preserve and will not go mouldy, sample soft texture, and color and luster is good.
In order to solve the above technical problems, the present invention is realized using following technical scheme：
A kind of preservation liquid of pathological tissue specimen, is mixed by weight ratio by following raw material：
2-5 parts of potassium sorbate, 10-20 parts of industrial alcohol, 1-3 parts of glycerine, 2-3 parts of glutaraldehyde, 8.5-9.5 parts of sodium chloride, nanometer
Silver-colored 0.6-1 parts, 10-20 parts of citric acid, 60-100 parts of water.
Preferably, the preservation liquid of above-mentioned pathological tissue specimen, is mixed by weight ratio by following raw material：
2 parts of potassium sorbate, 10 parts of industrial alcohol, 1 part of glycerine, 2 parts of glutaraldehyde, 8.5 parts of sodium chloride, 0.6 part of Nano Silver, citric acid
10 parts, 60 parts of water.
Preferably, the preservation liquid of above-mentioned pathological tissue specimen, is mixed by weight ratio by following raw material：
3.5 parts of potassium sorbate, 15 parts of industrial alcohol, 2 parts of glycerine, 2.5 parts of glutaraldehyde, 9 parts of sodium chloride, 0.8 part of Nano Silver, lemon
Sour 15 parts, 80 parts of water.
Preferably, the preservation liquid of above-mentioned pathological tissue specimen, is mixed by weight ratio by following raw material：
5 parts of potassium sorbate, 20 parts of industrial alcohol, 3 parts of glycerine, 3 parts of glutaraldehyde, 9.5 parts of sodium chloride, 1 part of Nano Silver, citric acid 20
Part, 100 parts of water.
The mass fraction of the glutaraldehyde is 50-52%.
The potassium sorbate is colourless flake shape crystalline powder, odorless, tasteless, soluble in water, ethanol.Potassium sorbate has
There are efficient anti-corrosive properties and bacteriostasis, and better heat stability, decomposition temperature is up to 270 DEG C, and the low advantage of toxicity, is state
Low toxicity, the efficient fresh-keeping preservative of border food and agricultural organization and the World Health Organization to various countries' keypoint recommendation；It to enzyme bacterium, saccharomycete,
Aerobic microorganism has obvious inhibiting effect, and antibacterial optimum pH is in 5.0-6.0, and its fungistatic effect is higher than benzoic acid 5-10 times,
Toxicity is only 1/5th of benzoic acid；And flavour of food products is had no adverse effects, be widely used in meat, fish, egg, bird product,
The anti-corrosive fresh-keeping of grape wine, fruit wine, dairy products etc..Anti-corrosion is played in preservation to human body specimen, sterilization, keeps primitive organism sample
The color and luster and wettability of tissue, prevent the effect gone mouldy.
The Nano Silver is that particle diameter is worked into nano level argent simple substance.Its particle diameter is generally 2-15nm, is in recent years
Come the strong nano inorganic anti-biotic material of the new, wide spectrum being widely studied and applied, safety, antibacterial activity, because physicochemical property is only
Greatly, its antibacterial effect is that other antiseptics are incomparable to special and specific surface area.Currently as new antiseptic medical treatment,
The fields such as building materials have obtained relatively broad application.Its surface is combined closely with protein molecule by nitrogen-atoms, can be to people
Body sample tissue protein carries out the crosslinking of appropriateness, and then plays fixed effect, keeps the original form and original of human body specimen
The pliability and toughness of beginning tissue.
The citric acid is nonpoisonous and tasteless organic acid, with very strong sterilization and antioxidation, can effectively kill blood
The bacterial spore polluted in liquid dialysis machine pipeline, has efficient antibacterial and holding biological sample primary colors to the preservation of biological sample
The effect in pool, beneficial to the preparation to preserving fluid for biological sample.
Compared with prior art, the advantage of the invention is that：
1st, specimen preserving liquid of the present invention is the liquid of water white transparency, is had no irritating odor, fungus and mildew resistance excellent performance；
2nd, Saving specimen liquid energy of the invention effectively keeps the tissue morphology and color and luster of protozoa sample, paint having no volatile substances,
Effect persistently, can be effectively reduced cost；
3rd, Novel specimen of the invention preserves liquid and is free of poisonous and harmful substance, and environmental protection mitigates the burden of environment.
Embodiment
Present disclosure is further illustrated using specific embodiment below.
Herein below is to combine specific preferred embodiment further description made for the present invention, it is impossible to assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Embodiment 1
A kind of preservation liquid of pathological tissue specimen, is mixed by weight ratio by following raw material：
Potassium sorbate 2g, industrial alcohol 10g, glycerine 1g, glutaraldehyde 2g, sodium chloride 8.5g, Nano Silver 0.6g, citric acid 10g, water
60g。
The mass fraction of the glutaraldehyde is 50%.
Embodiment 2
A kind of preservation liquid of pathological tissue specimen, is mixed by weight ratio by following raw material：
Potassium sorbate 3.5g, industrial alcohol 15g, 2 parts of glycerine, glutaraldehyde 2.5g, sodium chloride 9g, Nano Silver 0.8g, citric acid
15g, water 80g.
The mass fraction of the glutaraldehyde is 51%.
Embodiment 3
A kind of preservation liquid of pathological tissue specimen, is mixed by weight ratio by following raw material：
Potassium sorbate 5g, industrial alcohol 20g, glycerine 3g, glutaraldehyde 3g, sodium chloride 9.5g, Nano Silver 1g, citric acid 20g, water
100g。
The mass fraction of the glutaraldehyde is 52%.
Claims (5)
1. a kind of preservation liquid of pathological tissue specimen, it is characterized in that：It is mixed by weight ratio by following raw material：
2-5 parts of potassium sorbate, 10-20 parts of industrial alcohol, 1-3 parts of glycerine, 2-3 parts of glutaraldehyde, 8.5-9.5 parts of sodium chloride, nanometer
Silver-colored 0.6-1 parts, 10-20 parts of citric acid, 60-100 parts of water.
2. the preservation liquid of pathological tissue specimen according to claim 1, it is characterized in that：By following raw material by weight ratio
It is mixed：
2 parts of potassium sorbate, 10 parts of industrial alcohol, 1 part of glycerine, 2 parts of glutaraldehyde, 8.5 parts of sodium chloride, 0.6 part of Nano Silver, citric acid
10 parts, 60 parts of water.
3. the preservation liquid of pathological tissue specimen according to claim 1, it is characterized in that：By following raw material by weight ratio
It is mixed：
3.5 parts of potassium sorbate, 15 parts of industrial alcohol, 2 parts of glycerine, 2.5 parts of glutaraldehyde, 9 parts of sodium chloride, 0.8 part of Nano Silver, lemon
Sour 15 parts, 80 parts of water.
4. the preservation liquid of pathological tissue specimen according to claim 1, it is characterized in that：By following raw material by weight ratio
It is mixed：
5 parts of potassium sorbate, 20 parts of industrial alcohol, 3 parts of glycerine, 3 parts of glutaraldehyde, 9.5 parts of sodium chloride, 1 part of Nano Silver, citric acid 20
Part, 100 parts of water.
5. the preservation liquid of pathological tissue specimen according to claim 1, it is characterized in that：The mass fraction of the glutaraldehyde is
50-52%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710196330.6A CN106973888A (en) | 2017-03-29 | 2017-03-29 | A kind of preservation liquid of pathological tissue specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710196330.6A CN106973888A (en) | 2017-03-29 | 2017-03-29 | A kind of preservation liquid of pathological tissue specimen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106973888A true CN106973888A (en) | 2017-07-25 |
Family
ID=59338568
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710196330.6A Withdrawn CN106973888A (en) | 2017-03-29 | 2017-03-29 | A kind of preservation liquid of pathological tissue specimen |
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| Country | Link |
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| CN (1) | CN106973888A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107560918A (en) * | 2017-09-22 | 2018-01-09 | 漯河医学高等专科学校 | A kind of pathological tissue specimen fixer |
| CN108094408A (en) * | 2018-01-06 | 2018-06-01 | 济宁市第二人民医院 | A kind of preservation fixer of pathological tissue and preparation method thereof |
| CN110604126A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly pathological tissue fixing agent |
| CN111280161A (en) * | 2018-12-06 | 2020-06-16 | 西安培华学院 | Biological specimen preserving fluid and preparation method thereof |
| CN113170781A (en) * | 2021-04-16 | 2021-07-27 | 河南有道展览科技有限公司 | Plant-impregnated environment-friendly preservation solution |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2909837A1 (en) * | 2006-12-14 | 2008-06-20 | Labromex Ass Loi De 1901 | Tissue-preserving composition for embalming or medical use comprises sodium tetraborate, benzalkonium chloride, ascorbic acid, sodium chloride, benzyl alcohol, glycerol, sorbic acid and eosin |
| CN101473812A (en) * | 2009-01-24 | 2009-07-08 | 曹建明 | Antiseptic fixing liquid composition for cadaver sample and preparation method thereof |
| CN104365581A (en) * | 2014-11-05 | 2015-02-25 | 中山大学 | Novel specimen conservation solution |
-
2017
- 2017-03-29 CN CN201710196330.6A patent/CN106973888A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2909837A1 (en) * | 2006-12-14 | 2008-06-20 | Labromex Ass Loi De 1901 | Tissue-preserving composition for embalming or medical use comprises sodium tetraborate, benzalkonium chloride, ascorbic acid, sodium chloride, benzyl alcohol, glycerol, sorbic acid and eosin |
| CN101473812A (en) * | 2009-01-24 | 2009-07-08 | 曹建明 | Antiseptic fixing liquid composition for cadaver sample and preparation method thereof |
| CN104365581A (en) * | 2014-11-05 | 2015-02-25 | 中山大学 | Novel specimen conservation solution |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107560918A (en) * | 2017-09-22 | 2018-01-09 | 漯河医学高等专科学校 | A kind of pathological tissue specimen fixer |
| CN108094408A (en) * | 2018-01-06 | 2018-06-01 | 济宁市第二人民医院 | A kind of preservation fixer of pathological tissue and preparation method thereof |
| CN108094408B (en) * | 2018-01-06 | 2021-05-04 | 济宁市第二人民医院 | Pathological tissue preservation stationary liquid |
| CN110604126A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly pathological tissue fixing agent |
| CN111280161A (en) * | 2018-12-06 | 2020-06-16 | 西安培华学院 | Biological specimen preserving fluid and preparation method thereof |
| CN113170781A (en) * | 2021-04-16 | 2021-07-27 | 河南有道展览科技有限公司 | Plant-impregnated environment-friendly preservation solution |
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| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170725 |
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