CN113170781A - Plant-impregnated environment-friendly preservation solution - Google Patents
Plant-impregnated environment-friendly preservation solution Download PDFInfo
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- CN113170781A CN113170781A CN202110410088.4A CN202110410088A CN113170781A CN 113170781 A CN113170781 A CN 113170781A CN 202110410088 A CN202110410088 A CN 202110410088A CN 113170781 A CN113170781 A CN 113170781A
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- sorbic acid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- Agronomy & Crop Science (AREA)
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Abstract
The invention relates to the technical field of plant infusion, and discloses an environment-friendly plant infusion preservative solution which comprises a preservative solution formula, wherein the preservative solution formula comprises 1.2 parts of mixed solution of sorbic acid and potassium sorbate, 0.04 part of glycerol, 0.7 part of alcohol, 0.02 part of sodium chloride, 0.02 part of alum and 98.02 parts of purified water. The invention has the advantages that: replace formaldehyde as the antiseptic through using sorbic acid and potassium sorbate mixed solution that contains the percentage of hexa sorbic acid, through adjusting the proportion, make the ratio adjustment of sorbic acid and potassium sorbate reach the most suitable degree, good preservation effect has, make the flower that the plant soaked the sample can keep original morphological structure and inherent colour, the color and luster is bright-colored, the third dimension is strong, the form is lifelike, sorbic acid and potassium sorbate are all nontoxic and harmless, can not release toxic gas, use safelyr, compare formalin solution, when having the same preservation effect, can not cause irreversible injury to the sample preparation personnel, and is suitable for being generalized to use.
Description
Technical Field
The invention relates to the technical field of plant infusion, in particular to an environment-friendly preservation solution for plant infusion.
Background
Many efforts have been made and some results have been achieved in order to develop a better method for preparing plant infusion specimens, wherein the infusion method is a common method for preparing plant infusion specimens.
At present, common soaked specimens in the market are prepared by using formalin solution as preservation solution. The formalin solution is an aqueous solution of formaldehyde. As is well known, formaldehyde is a chemical substance harmful to human bodies, is colorless and has strong irritant odor, is easily dissolved in water and ethanol, is mainly used for stimulating skin mucosa and can be combined with protein, severe irritation and edema of respiratory tract, eye irritation, headache and the like can occur when the formaldehyde is inhaled at high concentration, and skin directly contacts the formaldehyde to cause allergic dermatitis, color spots and necrosis, bronchial asthma can be induced by inhaling the formaldehyde to cause gene mutation, nasopharyngeal tumors and the like, so that the problem of continuously solving the technical field of plant infusion at present is to develop the environment-friendly preservation solution which can be preserved for long time in primary color and is environment-friendly.
Based on the problems, the invention provides an environment-friendly plant-impregnated preservation solution, which is used for solving the existing problems.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the plant-impregnated environment-friendly preservation solution which has the advantages of environmental protection, no toxicity, safe use and the like and solves the problem of high toxicity of the existing formaldehyde preservation solution.
(II) technical scheme
In order to achieve the purpose of safe use, the invention provides the following technical scheme: the preservative fluid comprises a preservative fluid formula, wherein the preservative fluid formula comprises 1.2 parts of mixed solution of sorbic acid and potassium sorbate, 0.04 part of glycerol, 0.7 part of alcohol, 0.02 part of sodium chloride, 0.02 part of alum and 98.02 parts of purified water.
Preferably, the ratio of sorbic acid in the mixed solution of sorbic acid and potassium sorbate is six percent, and the concentration of alcohol is seventy-five percent.
The invention aims to solve another technical problem of providing a preparation method of plant-impregnated environment-friendly preservation solution, which comprises the following steps:
1) adding 1.2 parts of a mixed solution of sorbic acid containing hexasorbic acid and potassium sorbate, 0.7 part of seventy-five percent alcohol and 0.04 part of glycerin into a specimen bottle, and uniformly mixing;
2) adding one gram of sodium chloride and one gram of alum into a beaker, adding purified water, stirring until the sodium chloride and the alum are dissolved, and preparing a mixed solution accounting for 0.04 part of the preservation solution;
3) adding the mixed solution of sodium chloride and alum into a specimen bottle, then adding 98.02 parts of purified water, and stirring until the mixture is fused and not layered to finish the preparation.
(III) advantageous effects
Compared with the prior art, the invention provides the plant-impregnated environment-friendly preservation solution, which has the following beneficial effects:
this plant soaks environmental protection preservative solution, it replaces formaldehyde as the antiseptic to mix liquid through sorbic acid and the potassium sorbate that use to contain percentage hexa sorbic acid, through the adjustment proportion, make the ratio adjustment of sorbic acid and potassium sorbate to the most suitable degree, good preservation effect has, make the flower of plant soaks the sample can keep original morphological structure and inherent colour, bright in color and luster, the third dimension is strong, the form is lifelike, sorbic acid and potassium sorbate are all nontoxic and harmless simultaneously, can not release toxic gas, use safelyr, compare traditional formalin solution, when having the same preservation effect, can not cause irreversible injury to sample preparation personnel, and is suitable for being generalized to use.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: an environment-friendly plant-infused preservation solution comprises a preservation solution formula, wherein the preservation solution formula comprises 1.2 parts of mixed solution of sorbic acid and potassium sorbate, 0.04 parts of glycerol, 0.7 parts of alcohol, 0.02 parts of sodium chloride, 0.02 parts of alum and 98.02 parts of purified water.
Wherein, the ratio of sorbic acid in the mixed solution of sorbic acid and potassium sorbate accounts for six percent, and the concentration of alcohol is seventy-five percent.
The preparation method of the plant-impregnated environment-friendly preservation solution comprises the following steps:
1) adding 1.2 parts of a mixed solution of sorbic acid containing hexasorbic acid and potassium sorbate, 0.7 part of seventy-five percent alcohol and 0.04 part of glycerin into a specimen bottle, and uniformly mixing;
2) adding one gram of sodium chloride and one gram of alum into a beaker, adding purified water, stirring until the sodium chloride and the alum are dissolved, and preparing a mixed solution accounting for 0.04 part of the preservation solution;
3) adding the mixed solution of sodium chloride and alum into a specimen bottle, then adding 98.02 parts of purified water, and stirring until the mixture is fused and not layered to finish the preparation.
The embodiment has the advantages that the mixed solution of sorbic acid and potassium sorbate containing the hexa-sorbic acid is used for replacing formaldehyde as the preservative, the ratio of the sorbic acid to the potassium sorbate is adjusted to the most appropriate degree by adjusting the ratio, and the mixed solution has good preservation effect and is suitable for preserving green plants.
Example two: an environment-friendly plant-infused preservation solution comprises a preservation solution formula, wherein the preservation solution formula comprises 4 parts of boric acid, 1 part of zinc chloride, 20 parts of alcohol, 20 parts of a mixed solution of sorbic acid and potassium sorbate, 0.5 part of glycerol, 0.1 part of alum and 54.4 parts of purified water.
Wherein, the ratio of sorbic acid in the mixed solution of sorbic acid and potassium sorbate accounts for six percent, and the concentration of alcohol is seventy-five percent.
The preparation method of the plant-impregnated environment-friendly preservation solution comprises the following steps:
1) adding 4 parts of boric acid, 1 part of zinc chloride, 20 parts of alcohol, 20 parts of mixed solution of sorbic acid and potassium sorbate, 0.5 part of glycerol and 0.1 part of alum into a specimen bottle and uniformly mixing;
2) and adding 54.4 parts of purified water into the specimen bottle.
The advantage of this embodiment lies in having added 4 parts boric acid and 1 part zinc chloride, has got rid of the sodium chloride, can effectually resume the true qualities of red plant, prevents simultaneously to rot, is applicable to the preparation red plant specimen.
This example uses the premise that the red plants are first soaked in a fixative solution, which is a mixture of 0.98 parts of a mixture of sorbic acid and potassium sorbate containing hexa-sorbic acid, 0.74 parts of boric acid, and 98.28 parts of purified water, for 1-3 days.
Example three: an environment-friendly preservation solution prepared by plant infusion comprises a preservation solution formula, wherein the preservation solution formula comprises 17.3 parts of mixed solution of sorbic acid and potassium sorbate, 0.17 part of copper sulfate, 0.43 part of glycerol, 4.31 parts of ethanol, 0.09 part of alum, 0.09 part of sodium chloride and 77.61 parts of purified water.
The sorbic acid in the mixed solution of sorbic acid and potassium sorbate accounts for six percent, the ethanol concentration is ninety-five percent, and the copper sulfate concentration is five percent.
The preparation method of the plant-impregnated environment-friendly preservation solution comprises the following steps:
1) adding 7.3 parts of mixed solution of sorbic acid and potassium sorbate, 0.17 part of copper sulfate, 0.43 part of glycerin, 4.31 parts of ethanol, 0.09 part of alum, 0.09 part of sodium chloride and 77.61 parts of purified water into a sample bottle, and uniformly stirring by using a glass rod script.
The present example has the advantage of being suitable for the preservation of yellow as well as yellow-green plants.
The presupposition used in this example is that the specimen is firstly immersed in copper sulfate solution with five percent concentration for 1-2 days, then the specimen is placed in preservation solution for preservation after being cleaned, and if the fruit specimen is made, a small amount of preservation solution is firstly injected into the fruit before immersion, for example, the specimen is yellow persimmon, the specimen can be firstly immersed in 5 percent copper sulfate solution for 1-2 days and rinsed, then the specimen is immersed in 0.2 percent mixed solution of sorbic acid and potassium sorbate, a small amount of glycerol is added, then the specimen is fixed in the preservation solution, and the natural color is gradually recovered, and then the specimen can be preserved.
Example four: the plant-soaked environment-friendly preservation solution comprises a preservation solution formula, wherein the preservation solution formula comprises 4.12 parts of mixed solution of sorbic acid and potassium sorbate, 2.1 parts of saturated solution of sodium chloride, 0.02 part of alum, 4.12 parts of alcohol and 89.64 parts of purified water.
Wherein, the ratio of sorbic acid in the mixed solution of sorbic acid and potassium sorbate accounts for six percent, and the concentration of alcohol is seventy-five percent.
The preparation method of the plant-impregnated environment-friendly preservation solution comprises the following steps:
1) sequentially adding 4.12 parts of mixed solution of sorbic acid and potassium sorbate, 2.1 parts of saturated solution of sodium chloride, 0.02 part of alum and 4.12 parts of alcohol into 89.64 parts of purified water, and uniformly stirring;
the advantage of this embodiment lies in being applicable to the preparation of purple plant specimen, can effectively keep the true qualities of purple plant for a long time.
The premise used in the embodiment is that 16g of salt is added into 100ml of purified water and is fully stirred, the salt is completely dissolved soon, the clear saturated salt solution is poured out, 10ml of mixed solution of 6% sorbic acid and potassium sorbate in volume fraction is taken, 15ml of saturated salt solution is added, water is added to 100ml, the mixed solution of 6% sorbic acid and potassium sorbate is prepared into the saturated salt solution, and the saturated salt solution is fixed in a preservation solution, so that the purple plants in the preservation solution gradually recover the natural color.
The invention has the beneficial effects that: replace formaldehyde as the antiseptic through using sorbic acid and potassium sorbate mixed solution that contains six sorbic acid hundredths, through adjusting the proportion, make the proportion of sorbic acid and potassium sorbate adjust to the most suitable degree, good preservation effect has, make the flower that the plant soaked the sample can keep original morphological structure and inherent colour, bright-colored, the third dimension is strong, the form is lifelike, sorbic acid and potassium sorbate are all nontoxic and harmless simultaneously, can not release toxic gas, it is safer to use, compare traditional formalin solution, when having the same preservation effect, can not cause irreversible injury to sample preparation personnel, be fit for using widely
Typical cases are as follows: an environment-friendly plant-infused preservation solution comprises a preservation solution formula, wherein the preservation solution formula comprises 1.2 parts of mixed solution of sorbic acid and potassium sorbate, 0.04 parts of glycerol, 0.7 parts of alcohol, 0.02 parts of sodium chloride, 0.02 parts of alum and 98.02 parts of purified water.
Wherein, the ratio of sorbic acid in the mixed solution of sorbic acid and potassium sorbate accounts for six percent, and the concentration of alcohol is seventy-five percent.
The preparation method of the plant-impregnated environment-friendly preservation solution comprises the following steps:
1) adding 1.2 parts of a mixed solution of sorbic acid containing hexasorbic acid and potassium sorbate, 0.7 part of seventy-five percent alcohol and 0.04 part of glycerin into a specimen bottle, and uniformly mixing;
2) adding one gram of sodium chloride and one gram of alum into a beaker, adding purified water, stirring until the sodium chloride and the alum are dissolved, and preparing a mixed solution accounting for 0.04 part of the preservation solution;
3) adding the mixed solution of sodium chloride and alum into a specimen bottle, then adding 98.02 parts of purified water, and stirring until the mixture is fused and not layered to finish the preparation.
Compared with other embodiments, the fixing solution needs to be prepared separately, and the fixing solution can be directly soaked in the mixed solution of 6% sorbic acid and potassium sorbate, and the mixed solution of 6% sorbic acid and potassium sorbate is a raw material for preparing the preservation solution, so that the steps are simpler, the manufacturing efficiency is improved, and green plants in nature exist more widely, the application space is large, and the method is suitable for large-scale popularization.
All embodiments of the invention require pre-treatment of the plant before specimen preparation, the treatment content is as follows:
adding 10g of boric acid into 100ml of clean water, heating on an electric furnace and continuously stirring by a glass rod until the boric acid and the clean water are completely fused and boiled. Taking out one end of a flower branch by wearing a rubber glove, immersing one end of the flower in the solution, turning the flower for 1min continuously from left to right and up and down, taking out the flower, slightly washing the flower, and immersing the red flower in 2% tartaric acid solution for 10-20 min; the purple flower can be soaked in 2 percent aluminum sulfate solution for 10-20 min; soaking the white flowers in a zinc chloride solution for 10-20 min; soaking the daylily in 6% sulfurous acid solution for 10-20 min; the soaked flowers are taken out of the solution and washed and dehydrated.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. The plant-infused environment-friendly preservation solution comprises a preservation solution formula and is characterized in that the preservation solution formula is composed of 1.2 parts of mixed solution of sorbic acid and potassium sorbate, 0.04 part of glycerol, 0.7 part of alcohol, 0.02 part of sodium chloride, 0.02 part of alum and 98.02 parts of purified water.
2. The plant-infused environment-friendly preservation solution according to claim 1, wherein the ratio of sorbic acid in the mixture of sorbic acid and potassium sorbate is six percent, and the concentration of alcohol is seventy-five percent.
3. A method for preparing plant-infused environment-friendly preservation solution by using any one of claims 1-2, which is characterized by comprising the following steps:
1) adding 1.2 parts of a mixed solution of sorbic acid containing hexasorbic acid and potassium sorbate, 0.7 part of seventy-five percent alcohol and 0.04 part of glycerin into a specimen bottle, and uniformly mixing;
2) adding one gram of sodium chloride and one gram of alum into a beaker, adding purified water, stirring until the sodium chloride and the alum are dissolved, and preparing a mixed solution accounting for 0.04 part of the preservation solution;
3) adding the mixed solution of sodium chloride and alum into a specimen bottle, then adding 98.02 parts of purified water, and stirring until the mixture is fused and not layered to finish the preparation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110410088.4A CN113170781A (en) | 2021-04-16 | 2021-04-16 | Plant-impregnated environment-friendly preservation solution |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110410088.4A CN113170781A (en) | 2021-04-16 | 2021-04-16 | Plant-impregnated environment-friendly preservation solution |
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| CN113170781A true CN113170781A (en) | 2021-07-27 |
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Citations (4)
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|---|---|---|---|---|
| CN101218909A (en) * | 2008-01-28 | 2008-07-16 | 河南中医学院 | Original plant humid preparation producing method |
| CN103392694A (en) * | 2013-08-26 | 2013-11-20 | 中国医学科学院药用植物研究所海南分所 | Method for preparing tropical plant preserving humid preparation |
| CN106973888A (en) * | 2017-03-29 | 2017-07-25 | 桂林医学院 | A kind of preservation liquid of pathological tissue specimen |
| CN109169639A (en) * | 2018-08-09 | 2019-01-11 | 四川省农业科学院植物保护研究所 | A kind of tobacco rhizome disease dipping specimen saves liquid and Slide processing |
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2021
- 2021-04-16 CN CN202110410088.4A patent/CN113170781A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101218909A (en) * | 2008-01-28 | 2008-07-16 | 河南中医学院 | Original plant humid preparation producing method |
| CN103392694A (en) * | 2013-08-26 | 2013-11-20 | 中国医学科学院药用植物研究所海南分所 | Method for preparing tropical plant preserving humid preparation |
| CN106973888A (en) * | 2017-03-29 | 2017-07-25 | 桂林医学院 | A kind of preservation liquid of pathological tissue specimen |
| CN109169639A (en) * | 2018-08-09 | 2019-01-11 | 四川省农业科学院植物保护研究所 | A kind of tobacco rhizome disease dipping specimen saves liquid and Slide processing |
Non-Patent Citations (3)
| Title |
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| 上海钾盐工程技术研究中心: "《中国钾盐工业概览》", 31 July 2009, 上海交通大学出版社 * |
| 北京市科学技术情报研究所: "《世界切花保鲜新技术新配方》", 31 March 1988 * |
| 李凤丽 等: "新型绿色药用植物浸制标本保存液的发现与效果", 《河南科学》 * |
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