CN109169639A - A kind of tobacco rhizome disease dipping specimen saves liquid and Slide processing - Google Patents
A kind of tobacco rhizome disease dipping specimen saves liquid and Slide processing Download PDFInfo
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- CN109169639A CN109169639A CN201810903663.2A CN201810903663A CN109169639A CN 109169639 A CN109169639 A CN 109169639A CN 201810903663 A CN201810903663 A CN 201810903663A CN 109169639 A CN109169639 A CN 109169639A
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- CN
- China
- Prior art keywords
- tobacco
- rhizome
- disease
- rhizome disease
- tobacco rhizome
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of tobacco rhizome disease dipping specimens to save liquid and Slide processing, it is consisted of the following compositions: sodium hypochlorite 0.05~0.07%, glacial acetic acid 6.5~7.5%, alcohol 13~14.5%, ascorbic acid 0.3~0.5%, caryophyllus oil 0.05~0.15%.The following steps are included: (1) is impregnated in 6% copper sulphate dilution 28~32 hours after cleaning tobacco diseases rhizome with tap water；(2) step (1) treated rhizome is taken out and is stored in tobacco rhizome disease dipping specimen of the invention and saves in liquid.Five kinds of ingredient collective effects such as sodium hypochlorite of the present invention, glacial acetic acid, alcohol, ascorbic acid, caryophyllus oil can save the primary colors and form of tobacco rhizome disease sample, and reasonable percentage is colour-fast to the preservation steadily in the long term of tobacco rhizome disease sample between them, not scaling also functions to very important effect.
Description
Technical field
The invention belongs to preparation of specimen's technical fields more particularly to a kind of tobacco rhizome disease dipping specimen to save liquid and production
Method.
Background technique
The store method of existing tobacco rhizome disease dipping specimen is more, wherein mainly two kinds.One is by Tobacco Root
Stem disease evil sample is placed in copper acetate crystallization and acetic acid mixed diluting liquid and is put into formalin after boiling, protects in alcohol blend
It deposits；Another kind is that tobacco rhizome disease sample is placed in copper-bath, will be put into after rhizome disease sample boiling sulfurous acid,
It is saved in alcohol blend.Following point can occur for both methods to impregnate preservation sample: (1) in disease sample disposal process
Middle heat treatment liquid can volatilize, and distribute penetrating odor, seriously affect the health of people.(2) disease specimen preserving liquid
Main component formalin and sulfurous acid have certain toxicity.Formalin has very strong stimulation to human eye nose, is one
In class carcinogenic substance inventory, sulfurous acid has strong impulse effect to eyes, skin, mucous membrane and respiratory tract.(3) formalin, Asia
Sulfuric acid does not allow the stimulation of tobacco rhizome disease sample the primary colors of tobacco rhizomatous disease evil sample easy to maintain yet.(4) when saving
Between shortly will appear scaling, fade phenomena such as, cause scab not see Chu, Disease Characters are unobvious.
Summary of the invention
The technical problems to be solved by the invention are as follows: (1) at present formalin in tobacco rhizome disease sample dipping process,
The influence of sulfurous acid human health impregnates influence of the raffinate to environment.(2) tobacco rhizome disease Saving specimen time root more long afterwards
There is scaling colour fading in stem disease evil sample, and the holding time soon, can not understand and distinguish scab.(3) heating distributes thorn in treatment process
Swash property smell.
The technical solution of the present invention is as follows: a kind of tobacco rhizome disease dipping specimen saves liquid, it is by following mass percent
At being grouped as: sodium hypochlorite 0.05~0.07%, glacial acetic acid 6.5~7.5%, alcohol 13~14.5%, ascorbic acid 0.3~
0.5%, caryophyllus oil 0.05~0.15%, surplus are water.
On the other hand, the present invention also provides a kind of production methods of tobacco rhizome disease sample, comprising the following steps:
(1) it is impregnated in 6% copper sulphate dilution 28~32 hours after cleaning tobacco diseases rhizome with tap water；
(2) step (1) treated rhizome is taken out and is stored in tobacco rhizome disease dipping specimen of the invention and saves liquid
In.
Compared with prior art, the invention has the following advantages:
The present invention, which uses for tobacco rhizome disease sample to be put into copper sulphate dilution after soaking sterilization, places into hypochlorous acid
It is saved in the mixed liquor that sodium, glacial acetic acid, alcohol, ascorbic acid, caryophyllus oil form.Present invention safety prepares simple, Saving specimen
Time is long, can show the symptom feature of different parts, different phase.Sodium hypochlorite of the present invention, glacial acetic acid, alcohol, Vitamin C
Five kinds of ingredient collective effects such as acid, caryophyllus oil can save the primary colors and form of tobacco rhizome disease sample, reasonable between them
Percentage is colour-fast to the preservation steadily in the long term of tobacco rhizome disease sample, not scaling also functions to very important effect.
Specific embodiment
Under same preservation utensil and same preservation condition, is impregnated respectively with Examples 1 to 5 and comparative example 1~2 and save cigarette
Grass roots stem disease does harm to sample.Every group sets 5 repetitions, altogether in triplicate.Observation sample situation daily, whether there is or not colour fading, scaling phenomenon, diseases
Whether spot is obvious.The months for counting tobacco rhizome disease sample fades, scaling occurs time and preservation, finally to fade, fall
Skin, holding time length carry out the effect of each store method of overall merit.
Embodiment 1:
A kind of tobacco rhizome disease dipping specimen saves liquid and production method, comprising the following steps:
1. slightly modifying (making in its easy entry bottle and more beautiful) after tobacco rhizome disease sample tap water is cleaned, then soak
Stain regards rhizome size in 6% copper sulphate dilution and old tender degree is impregnated 28~32 hours.
2. the taking-up of tobacco rhizome disease sample is put into and is stored in sodium hypochlorite 0.05%, glacial acetic acid 7.5%, alcohol
14.5%, in the preservation liquid that ascorbic acid 0.3%, caryophyllus oil 0.05% form.
Embodiment 2:
A kind of tobacco rhizome disease dipping specimen saves liquid and production method, comprising the following steps:
1. slightly modifying (making in its easy entry bottle and more beautiful) after tobacco rhizome disease sample tap water is cleaned, then soak
Stain regards rhizome size in 6% copper sulphate dilution and old tender degree is impregnated 28~32 hours.
2. the taking-up of tobacco rhizome disease sample is put into and is stored in sodium hypochlorite 0.06%, glacial acetic acid 7.0%, alcohol
14.0%, in the preservation liquid that ascorbic acid 0.4%, caryophyllus oil 0.1% form.
Embodiment 3:
A kind of tobacco rhizome disease dipping specimen saves liquid and production method, comprising the following steps:
1. slightly modifying (making in its easy entry bottle and more beautiful) after tobacco rhizome disease sample tap water is cleaned, then soak
Stain impregnates view rhizome size and always tender degree 28~32 hours in 6% copper sulphate dilution.
2. the taking-up of tobacco rhizome disease sample is put into and is stored in sodium hypochlorite 0.07%, glacial acetic acid 6.5%, alcohol
13.5%, in the preservation liquid that ascorbic acid 0.5%, caryophyllus oil 0.15% form.
Embodiment 4:
A kind of tobacco rhizome disease dipping specimen saves liquid and production method, comprising the following steps:
1. slightly modifying (making in its easy entry bottle and more beautiful) after tobacco rhizome disease sample tap water is cleaned, then soak
Stain impregnates view rhizome size and always tender degree 28~32 hours in 6% copper sulphate dilution.
2. the taking-up of tobacco rhizome disease sample is put into and is stored in sodium hypochlorite 0.07%, glacial acetic acid 6.5%, alcohol
13.5%, in the preservation liquid that caryophyllus oil 0.15% forms.
Embodiment 5:
A kind of tobacco rhizome disease dipping specimen saves liquid and production method, comprising the following steps:
1. slightly modifying (making in its easy entry bottle and more beautiful) after tobacco rhizome disease sample tap water is cleaned, then soak
Stain impregnates view rhizome size and always tender degree 28~32 hours in 6% copper sulphate dilution.
2. the taking-up of tobacco rhizome disease sample is put into and is stored in sodium hypochlorite 0.09%, glacial acetic acid 5.5%, alcohol
14.0%, in the preservation liquid that ascorbic acid 0.25%, caryophyllus oil 0.15% form.
Comparative example 1:
A kind of tobacco rhizome disease dipping specimen saves liquid and production method, comprising the following steps:
1. slightly modifying (making in its easy entry bottle and more beautiful) after tobacco rhizome disease sample tap water is cleaned, then soak
Stain regards rhizome size in 6% copper sulphate dilution and old tender degree is impregnated 28~32 hours.
2. being stored in 1000 milliliters, 16 grams 20 milliliters of the concentrated sulfuric acid, water solid sulfurous after tobacco rhizome disease sample is taken out
In the mixed liquor that sour sodium mixes well.
Comparative example 2:
A kind of tobacco rhizome disease dipping specimen saves liquid and production method, comprising the following steps:
1. slightly modifying (making in its easy entry bottle and more beautiful) after tobacco rhizome disease sample tap water is cleaned, then soak
Stain regards rhizome size in 6% copper sulphate dilution and old tender degree is impregnated 28~32 hours.
2. being stored in 25 milliliters of formaldehyde, 150 milliliters of alcohol, 1000 milliliters of water after tobacco rhizome disease sample is taken out sufficiently
In the mixed liquor of mixing.
Embodiment effect:
Tobacco rhizome disease specimen preserving liquid preservation effect compares
To tobacco rhizomatous disease prepared in tobacco rhizome disease sample and comparative example prepared in Examples 1 to 3
Evil sample stores preservation under identical environmental condition.Wherein the tobacco rhizome disease sample in comparative example is at 10 months or so
Phenomena such as there have been scaling, colour fading, unobvious scabs.And the embodiment of the present invention 1~3 saves the sample in liquid and is kept for 24 months
Still it is fresh as before, scab it is obvious, there is no discolouration phenomena, 4 tobacco rhizome disease of embodiment Saving specimen 10 months start
Colour fading, scab are unobvious, and 5 tobacco rhizome disease of embodiment begins with slight colour fading in Saving specimen 15 months.
Comparing the shortcomings that existing plant roots and stems sample dipping saves and deficiency, the present invention has following beneficial effect: this
The tobacco rhizome disease Slide processing of invention is easy to operate, and the made tobacco rhizome disease Saving specimen time is longer,
Experience 36 months still fresh as before and environmentally protective, reduces formaldehyde, sulfurous acid to the pollution of environment and to operator
The influence of body.
The specific embodiment of the application above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
The limitation to the application protection scope therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, under the premise of not departing from technical scheme design, various modifications and improvements can be made, these belong to this
The protection scope of application.
Claims (2)
1. a kind of tobacco rhizome disease dipping specimen saves liquid, which is characterized in that it is by following mass percent at being grouped as:
Sodium hypochlorite 0.05~0.07%, glacial acetic acid 6.5~7.5%, alcohol 13~14.5%, ascorbic acid 0.3~0.5%, cloves
Oil 0.05~0.15%, surplus is water.
2. a kind of production method of tobacco rhizome disease sample, which comprises the following steps:
(1) it is impregnated in 6% copper sulphate dilution 28~32 hours after cleaning tobacco diseases rhizome with tap water；
(2) step (1) treated rhizome taking-up tobacco rhizome disease dipping specimen described in claim 1 is stored in save
In liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810903663.2A CN109169639B (en) | 2018-08-09 | 2018-08-09 | Tobacco rhizome disease dipping specimen preservation solution and specimen preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810903663.2A CN109169639B (en) | 2018-08-09 | 2018-08-09 | Tobacco rhizome disease dipping specimen preservation solution and specimen preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109169639A true CN109169639A (en) | 2019-01-11 |
| CN109169639B CN109169639B (en) | 2021-01-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810903663.2A Expired - Fee Related CN109169639B (en) | 2018-08-09 | 2018-08-09 | Tobacco rhizome disease dipping specimen preservation solution and specimen preparation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113170781A (en) * | 2021-04-16 | 2021-07-27 | 河南有道展览科技有限公司 | Plant-impregnated environment-friendly preservation solution |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283718A (en) * | 2013-07-04 | 2013-09-11 | 云南农业大学 | Method for manufacturing tobacco disease specimen |
| CN103404510A (en) * | 2013-08-22 | 2013-11-27 | 湖北省烟草公司恩施州公司 | Method for making tobacco rhizome disease specimens |
| CN103548818A (en) * | 2013-11-25 | 2014-02-05 | 中国热带农业科学院香料饮料研究所 | Preparation method for orchidaceae succulent plant dipping specimen |
| CN103766328A (en) * | 2014-01-14 | 2014-05-07 | 云南省烟草农业科学研究院 | Manufacturing method for plant leaf dipping specimen |
-
2018
- 2018-08-09 CN CN201810903663.2A patent/CN109169639B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283718A (en) * | 2013-07-04 | 2013-09-11 | 云南农业大学 | Method for manufacturing tobacco disease specimen |
| CN103404510A (en) * | 2013-08-22 | 2013-11-27 | 湖北省烟草公司恩施州公司 | Method for making tobacco rhizome disease specimens |
| CN103548818A (en) * | 2013-11-25 | 2014-02-05 | 中国热带农业科学院香料饮料研究所 | Preparation method for orchidaceae succulent plant dipping specimen |
| CN103766328A (en) * | 2014-01-14 | 2014-05-07 | 云南省烟草农业科学研究院 | Manufacturing method for plant leaf dipping specimen |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113170781A (en) * | 2021-04-16 | 2021-07-27 | 河南有道展览科技有限公司 | Plant-impregnated environment-friendly preservation solution |
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| CN109169639B (en) | 2021-01-19 |
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| PB01 | Publication | ||
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| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Xu Inventor after: Chen Song Inventor after: Chen Qingdong Inventor after: Liu Zhenxing Inventor after: Zhang Zongjin Inventor after: Yan Fangfang Inventor after: Zhang Ruiping Inventor after: Zhang Yingjie Inventor after: Hu Wei Inventor after: Chen Qianying Inventor after: Lei Qiang Inventor after: Yu Wei Inventor after: Liu Changhua Inventor after: Xiao Jun Inventor after: Wu Bin Inventor after: Qu Jiankang Inventor after: Yu Xiangwen Inventor after: Li Bin Inventor before: Liu Xu Inventor before: Liu Changhua Inventor before: Xiao Jun Inventor before: Wu Bin Inventor before: Chen Song Inventor before: Chen Qingdong Inventor before: Hu Wei Inventor before: Chen Qianying |
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| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190606 Address after: 610000 No. 20 Jingjusi Road, Chengdu City, Sichuan Province Applicant after: INSTITUTE OF PLANT PROTECTION, SICHUAN ACADEMY OF AGRICULTURAL SCIENCES Applicant after: CHINA NATIONAL TOBACCO CORPORATION SICHUAN Applicant after: PANZHIHUA CORPORATION OF SICHUAN PROVINCE TOBACCO MONOPOLY ADMINISTRATION Address before: No. 20 Jingjusi Road, Chengdu City, Sichuan Province Applicant before: INSTITUTE OF PLANT PROTECTION, SICHUAN ACADEMY OF AGRICULTURAL SCIENCES |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210119 Termination date: 20210809 |