CN110050799A - A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application - Google Patents

A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application Download PDF

Info

Publication number
CN110050799A
CN110050799A CN201910230717.8A CN201910230717A CN110050799A CN 110050799 A CN110050799 A CN 110050799A CN 201910230717 A CN201910230717 A CN 201910230717A CN 110050799 A CN110050799 A CN 110050799A
Authority
CN
China
Prior art keywords
control agent
wood vinegar
parts
powder
tobacco
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910230717.8A
Other languages
Chinese (zh)
Inventor
周建云
王晓
唐泽兵
覃青青
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guiyang Office of Guizhou Tobacco Corp
Original Assignee
Guiyang Office of Guizhou Tobacco Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guiyang Office of Guizhou Tobacco Corp filed Critical Guiyang Office of Guizhou Tobacco Corp
Priority to CN201910230717.8A priority Critical patent/CN110050799A/en
Publication of CN110050799A publication Critical patent/CN110050799A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • A01N61/02Mineral oils; Tar oils; Tar; Distillates, extracts or conversion products thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/12Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/22Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/36Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/48Zingiberaceae [Ginger family], e.g. ginger or galangal
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B1/00Preparation of tobacco on the plantation
    • A24B1/02Arrangements in barns for preparatory treatment of the tobacco, e.g. with devices for drying
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/20Biochemical treatment

Abstract

The present invention relates to biological prevention and control agent technical fields, and in particular to a kind of biological prevention and control agent containing wood vinegar and its application, preparation and application.Biological prevention and control agent of the invention includes following component: wood vinegar, zanthoxylum powder, Paprika, ginger powder, Herba Menthae powder, thyme powder, sweet wormwood powder, bay leaf powder, madder powder and complex micro organism fungicide, and the complex micro organism fungicide includes serratia marcescens, bacillus subtilis, bacillus amyloliquefaciens, Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.The biological agent is prepared using advanced method, the prevention and treatment gone mouldy applied to tobacco black shank and tobacco leaf storage phase, it is used in the different phase of tobacco growing and storage period by distinct methods, it can be effectively reduced the disease incidence and tobacco mildew rate of tobacco black shank, chemical prevention is reduced to the pollution of environment and tobacco leaf and the loss of tobacco leaf, and biological prevention and control agent dosage of the invention is less, the degradable noresidue of ingredient, it is environmentally protective, it is not likely to produce drug resistance.

Description

A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application
Technical field
The present invention relates to biological prevention and control agent technical fields, and in particular to a kind of biological prevention and control agent containing wood vinegar and its application, Preparation and application.
Background technique
Tobacco black shank is one of the important disease occurred during tobacco fertility, can be occurred with field period in seedling stage, main Cause harm basal part of stem, root and blade, each main production cigarette district in China has different degrees of generation, endangers heavier.Plant disease incidence Less serious case 5-12%, serious plot disease incidence reaches 30% or more, or even causes to have no harvest, in recent years due to some local continuous cropping tobacco field faces Product expands, and it is spring cigarette that the continuous cropping time limit, which extends or change winter cigarette, and the disease incidence of tobacco black shank has the gesture of obvious rising.In addition to this, The natural alcoholization storage period is long after tobacco leaf harvest, since tobacco leaf hygroscopicity is strong, and containing needed for multiple-microorganism growth in tobacco leaf Nutriment, tobacco leaf easily breeds various microorganisms and goes mouldy in storage.
Tobacco black shank relies primarily on chemical prevention at present, but increases the resistance of pathogen gradually using chemical agent year after year By force, it needs that dosage is continuously improved, and preventive effect tends not to pollution that is satisfactory, but also will cause environment and agricultural product. And the sterilization of the chemical anti mildew agents such as common benzoic acid, sodium benzoate, sorbic acid and propionic acid, antibacterial effect in tobacco industry at present After power is smaller, and dosage is larger, and pipe tobacco is added in these chemical agents, when cigarette is burnt and sucked because Pintsch process can be generated to people The harmful gas of body.
Wood vinegar be the biomass such as timber in distillation apparatus after destructive distillation derived steam gas mixture through condensing and separating A kind of bronzing liquid obtained afterwards agriculturally has improvement soil, prevention and treatment pest and disease damage, promotes plant growth and is improving product The effects of quality, but the effect that wood vinegar prevention and treatment tobacco black shank is administered alone during tobacco planting is poor, also has no wood The vinegar liquid report mould proof in tobacco leaf storage phase energy.
Summary of the invention
Against the above technical problems, the object of the present invention is to provide a kind of biological prevention and control agent containing wood vinegar and its application, Preparation and application reduces pollution of the chemical prevention to environment and tobacco leaf, and improve to reduce the disease incidence of tobacco black shank Tobacco leaf storage phase mildew phenomena reduces tobacco mildew rate, reduces tobacco leaf loss.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 50-100 parts of wood vinegar, Chinese prickly ash 1-5 parts of powder, 1-5 parts of Paprika, 1-5 parts of ginger powder, 1-5 parts of Herba Menthae powder, 1-5 parts of thyme powder, 1-5 parts of sweet wormwood powder, basyleave 1-5 parts of powder, 1-5 parts of madder powder and 10-20 parts of complex micro organism fungicide, the complex micro organism fungicide includes Serratia Bacterium, bacillus subtilis, bacillus amyloliquefaciens, Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
Further, in the complex micro organism fungicide include serratia marcescens 1-4 parts, 3-5 parts of bacillus subtilis, 1-2 parts of 3-5 parts of bacillus amyloliquefaciens, 2-4 parts of Rhodopseudomonas palustris and wine red finger sporangiocyst bacterium.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind It is dry, it is pulverized and mixed with pulverizer, obtains mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
Further, 20 meshes are crossed after the mixed plant powder mixing in the step (1).
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match The seed-soaking liquid that the biological prevention and control agent mass concentration is 0.5-1g/L is made, seed is placed in seed-soaking liquid and impregnates 30-60min, is taken out It sows after natural drying.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: after tobacco transplant, often It uses mass concentration to spray tobacco cauline leaf for the biological prevention and control agent solution of 1-3g/L every 5-7d, sprays 2-3 times altogether;Every 7- 10d uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.3-0.5g/L, pours 2-3 times altogether.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: tobacco leaf picking is through drying Or after baking, before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, fumigated using the biological prevention and control agent Processing, usage amount 5-10g/m3
Further, the time of the suffocating treatment is 24-48h.
Compared with prior art, the invention has the following beneficial effects:
(1) wood vinegar soak extraction effective component is added after shining dry grinding in selection various plants, can improve itself and wood The control efficiency that vinegar liquid goes mouldy to tobacco black shank and tobacco leaf storage phase, with clear water or ordinary organic solvents such as methanol, ethyl alcohol or The soak extractions such as acetone are without this effect.The leaching efficiency that can improve effective component is impregnated after sieving again.Contain in Chinese prickly ash and hot red pepper The plurality of active ingredients such as volatile oil, alkaloid, organic acid, flavonoids and cumarin contain curcumin, gingerol and ginger in ginger The effective components such as essential oil, have the effect of Antimicrobial preservative, three be manured into soil in can also be to root-knot nematode, cutworm, mole cricket, gold The subterranean pest-insects such as needle worm and grub play avoidance effect, reduce because subterranean pest-insect black shank pathogen caused by root of biting is invaded Dye;Contain menthol and menthol in peppermint, thyme essential oil and Thymol are contained in thyme, contains sweet wormwood in sweet wormwood Element, sitosterol and stigmasterol, basyleave contain there are many chromocor compound, contain rubican, munjistin, Physcion in madder Equal ingredients can be formed after chelating to tobacco black shank pathogen after above a variety of different plant societys are extracted with wood vinegar There is the ingredient of killing or inhibiting effect with the mould generated during storage cigarette, such as a variety of alkaloids have pathogen stronger Permeability can directly kill pathogen, and alkaloid can also destroy the cell wall of pathogen and inhibit mycelia growth, make pathogen It loses activity, reduces infection rate;Special composition in various plants can destroy the oxidation-reduction process of pathogen, reach sterilization and make With;Wood vinegar soak after extraction also contains various trace elements, as contained in copper, zinc, calcium and sulphur and wood vinegar More than 100 kinds of organic acids, phenols, ketones and alcohols substance, can promote tobacco growing, strengthen immunity and disease resistance, thus indirectly Play antibacterial, disease-resistant effect.Wood vinegar and effective ingredients in plant can also accelerate beneficial microorganism breeding, with complex microorganism Microbial inoculum compounding application can improve beneficial bacterium to the antagonistic effect of pathogen.
(2) tobacco seed is placed in the seed-soaking liquid that biological prevention and control agent mass concentration is 0.5-1g/L after impregnating to dry and is carried out again Sowing can form one layer of defence film in the surface of the seed, and promote root system strong, prevent pathogen from invading from the surface of the seed or young root Cause disease.Pathogen infection blade, petiole and stem can be prevented by spraying cauline leaf using the biological prevention and control agent after dilution in tobacco in seedling stage Portion, moreover it is possible to tobacco plant chlorophyll content is improved, boosts metabolism and is accumulated with stem's lignin, improves stalk mechanical strength, Reduce the infection probability of pathogen;Tobacco plant root is poured using the biological prevention and control agent after dilution, can be promoted in soil beneficial to micro- The breeding of biology, the growth and communication process of antagonism pathogen.Tobacco leaf picking is stored after sunning or baking entering warehouse Before, it is fumigated in warehouse using biological prevention and control agent, the effective component in wood vinegar and various plants can be made to infiltrate into closed Storage space can be eliminated effectively and store interior hiding a variety of pathogens and mould, such as aspergillus, mould, Mucor and trichoderma are micro- Biology prevents tobacco leaf from going mouldy in storage.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention, Technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is the present invention one Divide embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making Every other embodiment obtained, shall fall within the protection scope of the present invention under the premise of creative work.
Wood vinegar used in following embodiment, Chinese prickly ash, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave, madder Grass, serratia marcescens, bacillus subtilis, bacillus amyloliquefaciens, Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine are It is commercially available.
Embodiment 1
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 50 parts of wood vinegar, zanthoxylum powder 1 Part, 3 parts of Paprika, 5 parts of ginger powder, 3 parts of Herba Menthae powder, 5 parts of thyme powder, 1 part of sweet wormwood powder, 3 parts of bay leaf powder, 1 part of madder powder With 10 parts of complex micro organism fungicide, include in the complex micro organism fungicide 1 part of serratia marcescens, 3 parts of bacillus subtilis, 1 part of 3 parts of bacillus amyloliquefaciens, 2 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match The seed-soaking liquid that the biological prevention and control agent mass concentration is 0.5g/L is made, seed is placed in seed-soaking liquid and impregnates 60min, nature is taken out and dries in the air It is sowed after dry;After tobacco transplant, mass concentration is used to spray tobacco cauline leaf for the biological prevention and control agent solution of 3g/L every 5d, It sprays altogether 3 times;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.3g/L every 8d, pours 2 times altogether; Tobacco leaf picking before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, the life is used after sunning or baking Anti- preparation carries out suffocating treatment, usage amount 5g/m3, the time of the suffocating treatment is for 24 hours.
Embodiment 2
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 75 parts of wood vinegar, zanthoxylum powder 3 Part, 5 parts of Paprika, 1 part of ginger powder, 5 parts of Herba Menthae powder, 1 part of thyme powder, 3 parts of sweet wormwood powder, 5 parts of bay leaf powder, 3 parts of madder powder With 15 parts of complex micro organism fungicide, include in the complex micro organism fungicide 1 part of serratia marcescens, 4 parts of bacillus subtilis, 2 parts of 4 parts of bacillus amyloliquefaciens, 4 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match The seed-soaking liquid that the biological prevention and control agent mass concentration is 0.75g/L is made, seed is placed in seed-soaking liquid and impregnates 45min, takes out nature It is sowed after drying;After tobacco transplant, mass concentration is used to spray tobacco stem for the biological prevention and control agent solution of 2g/L every 6d Leaf sprays 2 times altogether;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.4g/L every 10d, pours altogether 2 times;Tobacco leaf picking before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, institute is used after sunning or baking It states biological prevention and control agent and carries out suffocating treatment, usage amount 7.5g/m3, the time of the suffocating treatment is 36h.
Embodiment 3
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 100 parts of wood vinegar, zanthoxylum powder 5 Part, 1 part of Paprika, 3 parts of ginger powder, 1 part of Herba Menthae powder, 3 parts of thyme powder, 5 parts of sweet wormwood powder, 1 part of bay leaf powder, 5 parts of madder powder With 20 parts of complex micro organism fungicide, include in the complex micro organism fungicide 4 parts of serratia marcescens, 5 parts of bacillus subtilis, 2 parts of 5 parts of bacillus amyloliquefaciens, 4 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match The seed-soaking liquid that the biological prevention and control agent mass concentration is 1g/L is made, seed is placed in seed-soaking liquid and impregnates 30min, takes out naturally dry After sow;After tobacco transplant, mass concentration is used to spray tobacco cauline leaf for the biological prevention and control agent solution of 1g/L every 7d, altogether It sprays 2 times;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.5g/L every 9d, pours 3 times altogether;Cigarette Leaf harvests after sunning or baking, before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, the biological and ecological methods to prevent plant disease, pests, and erosion is used Preparation carries out suffocating treatment, usage amount 10g/m3, the time of the suffocating treatment is 48h.
Embodiment 4
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 50 parts of wood vinegar, zanthoxylum powder 3 Part, 1 part of Paprika, 5 parts of ginger powder, 3 parts of Herba Menthae powder, 5 parts of thyme powder, 1 part of sweet wormwood powder, 1 part of bay leaf powder, 5 parts of madder powder With 13 parts of complex micro organism fungicide, include in the complex micro organism fungicide 2 parts of serratia marcescens, 3 parts of bacillus subtilis, 1 part of 4 parts of bacillus amyloliquefaciens, 3 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match The seed-soaking liquid that the biological prevention and control agent mass concentration is 0.5g/L is made, seed is placed in seed-soaking liquid and impregnates 60min, nature is taken out and dries in the air It is sowed after dry;After tobacco transplant, mass concentration is used to spray tobacco cauline leaf for the biological prevention and control agent solution of 1g/L every 5d, It sprays altogether 3 times;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.5g/L every 7d, pours 3 times altogether; Tobacco leaf picking before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, the life is used after sunning or baking Anti- preparation carries out suffocating treatment, usage amount 5g/m3, the time of the suffocating treatment is for 24 hours.
Embodiment 5
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 75 parts of wood vinegar, zanthoxylum powder 1 Part, 5 parts of Paprika, 3 parts of ginger powder, 3 parts of Herba Menthae powder, 1 part of thyme powder, 5 parts of sweet wormwood powder, 3 parts of bay leaf powder, 3 parts of madder powder With 17 parts of complex micro organism fungicide, include in the complex micro organism fungicide 3 parts of serratia marcescens, 5 parts of bacillus subtilis, 2 parts of 4 parts of bacillus amyloliquefaciens, 3 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match The seed-soaking liquid that the biological prevention and control agent mass concentration is 0.75g/L is made, seed is placed in seed-soaking liquid and impregnates 45min, takes out nature It is sowed after drying;After tobacco transplant, mass concentration is used to spray tobacco stem for the biological prevention and control agent solution of 3g/L every 6d Leaf sprays 3 times altogether;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.4g/L every 8d, pours 2 altogether It is secondary;Tobacco leaf picking before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, institute is used after sunning or baking It states biological prevention and control agent and carries out suffocating treatment, usage amount 7.5g/m3, the time of the suffocating treatment is 36h.
Embodiment 6
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 100 parts of wood vinegar, zanthoxylum powder 5 Part, 3 parts of Paprika, 1 part of ginger powder, 1 part of Herba Menthae powder, 5 parts of thyme powder, 3 parts of sweet wormwood powder, 5 parts of bay leaf powder, 1 part of madder powder With 13 parts of complex micro organism fungicide, include in the complex micro organism fungicide 2 parts of serratia marcescens, 4 parts of bacillus subtilis, 1 part of 3 parts of bacillus amyloliquefaciens, 3 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match The seed-soaking liquid that the biological prevention and control agent mass concentration is 1g/L is made, seed is placed in seed-soaking liquid and impregnates 30min, takes out naturally dry After sow;After tobacco transplant, mass concentration is used to spray tobacco cauline leaf for the biological prevention and control agent solution of 2g/L every 7d, altogether It sprays 2 times;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.3g/L every 9d, pours 3 times altogether;Cigarette Leaf harvests after sunning or baking, before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, the biological and ecological methods to prevent plant disease, pests, and erosion is used Preparation carries out suffocating treatment, usage amount 10g/m3, the time of the suffocating treatment is 48h.
Medicament is not used in comparative example 1.
Comparative example 2 prevents and treats tobacco black shank using 30% first frost hymexazol of high-tech, 600 times of liquid.
Comparative example 3 is gone mouldy using the sodium benzoate prevention and treatment tobacco leaf storage phase.
Comparative example 4 is the difference from embodiment 1 is that be used only wood vinegar.
Comparative example 5 impregnates the soak that plant component obtains the difference from embodiment 1 is that clear water is used only.
Comparative example 6 is the difference from embodiment 1 is that be used only complex micro organism fungicide.
Comparative example 7 is the difference from embodiment 1 is that biological prevention and control agent is only used in seed soaking.
Comparative example 8 is the difference from embodiment 1 is that biological prevention and control agent only uses after transplanting.
Comparative example 9 is the difference from embodiment 1 is that biological prevention and control agent is only used in storage period.
Balck shank disease incidence and tobacco mildew rate are investigated in tobacco growing and storage, as a result such as following table.
1 tobacco growing phase of table balck shank disease incidence and storage period tobacco mildew rate
From the data in table 1, it can be seen that preventing with the comparative example 1 of unused medicament and using 30% 600 times of liquid of first frost hymexazol of high-tech The comparative example 2 for controlling tobacco black shank is compared, and the disease incidence of tobacco black shank is substantially reduced in embodiment 1-6, is averagely reduced 8.97% and 3.08%.The comparative example 3 of tobacco leaf storage phase mildew is prevented and treated with the comparative example 1 of unused medicament and using sodium benzoate It compares, storage period, tobacco mildew rate also decreased significantly in embodiment 1-6, averagely reduce 1.298% and 0.664%.Explanation Compared with conventional control method, using biological prevention and control agent of the invention to reduction tobacco black shank disease incidence and storage period tobacco mildew Rate has obvious effect.
Compared with embodiment 1 and comparative example 2, plant component is impregnated using only the comparative example 4 of wood vinegar, using only clear water and is obtained To soak comparative example 5 and using only the disease incidence of tobacco black shank in the comparative example 6 of complex micro organism fungicide it is all higher, But lower than the comparative example 1 not being administered, illustrate that three in the biological prevention and control agent kinds of ingredient exclusive uses have certain prevention and treatment cigarette The effect of careless balck shank, but effect is worse than general chemistry prevention and treatment, and the effect that complex micro organism fungicide is used alone in three kinds of ingredients Fruit is best.Compared with embodiment 1 and comparative example 3, it is higher than embodiment using only tobacco mildew rate in a kind of comparative example 4-6 of ingredient 1 but it is lower than comparative example 3, illustrates that the effect for having certain reduction tobacco mildew rate is used alone in three in the biological prevention and control agent kinds of ingredients Fruit, and plant component clear water soak effect therein is not so good as common preservatives, but singly applies wood vinegar and singly apply composite microbial The effect of object microbial inoculum is superior to common preservatives.
Compared with Example 1, biological prevention and control agent is only used after transplanting using the comparative example 7 of biological prevention and control agent and only in seed soaking Comparative example 8 in tobacco black shank disease incidence and storage period tobacco mildew rate it is higher, but lower than the comparative example that is not administered 1, illustrate that the biological prevention and control agent only also has certain reduction balck shank disease incidence and storage in the use of a certain period of tobacco growing phase The effect of phase tobacco mildew rate.And only storage period using biological prevention and control agent 9 tobacco black shank disease incidence of comparative example be not administered Comparative example 1 without significant difference, tobacco mildew rate is higher than embodiment 1 but is lower than other comparative examples, illustrates according to the invention make The effect for reducing balck shank disease incidence and storage period tobacco mildew rate with method is best.
In conclusion a kind of biological prevention and control agent containing wood vinegar of the invention, in tobacco growing and the not same order of storage period Section is used by distinct methods, can be effectively reduced the disease incidence and tobacco mildew rate of tobacco black shank, reduces chemical prevention to ring The pollution of border and tobacco leaf and the loss of tobacco leaf, and biological prevention and control agent dosage of the invention is less, the degradable noresidue of ingredient, green ring It protects, is not likely to produce drug resistance.

Claims (9)

1. a kind of biological prevention and control agent containing wood vinegar, which is characterized in that including following component in parts by weight: wood vinegar 50- 100 parts, 1-5 parts of zanthoxylum powder, 1-5 parts of Paprika, 1-5 parts of ginger powder, 1-5 parts of Herba Menthae powder, 1-5 parts of thyme powder, sweet wormwood powder 1-5 Part, 1-5 parts of bay leaf powder, 1-5 parts of madder powder and 10-20 parts of complex micro organism fungicide, the complex micro organism fungicide include viscous Matter Serratieae, bacillus subtilis, bacillus amyloliquefaciens, Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
2. a kind of biological prevention and control agent containing wood vinegar according to claim 1, which is characterized in that the composite microbial bacteria It include serratia marcescens 1-4 parts, 3-5 parts of bacillus subtilis, 3-5 parts of bacillus amyloliquefaciens, the red false unit cell in marsh in agent 1-2 parts of 2-4 parts of bacterium and wine red finger sporangiocyst bacterium.
3. a kind of preparation method of the biological prevention and control agent containing wood vinegar as claimed in claim 1 or 2, which is characterized in that including Following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried and is air-dried, used Pulverizer is pulverized and mixed, and obtains mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
4. a kind of preparation method of biological prevention and control agent containing wood vinegar according to claim 3, which is characterized in that the step Suddenly 20 meshes are crossed after the mixed plant powder mixing in (1).
5. a kind of application of the biological prevention and control agent containing wood vinegar as claimed in claim 1 or 2, which is characterized in that described to contain The prevention and treatment that the biological prevention and control agent of wood vinegar is applied to tobacco black shank and the tobacco leaf storage phase goes mouldy.
6. a kind of application method of the biological prevention and control agent containing wood vinegar as claimed in claim 1 or 2, which is characterized in that described Application method specifically: before tobacco sowing, the seed-soaking liquid that the biological prevention and control agent mass concentration is 0.5-1g/L is prepared, by seed It is placed in seed-soaking liquid and impregnates 30-60min, taking-up is sowed after natural drying.
7. a kind of application method of the biological prevention and control agent containing wood vinegar as claimed in claim 1 or 2, which is characterized in that described Application method specifically: after tobacco transplant, use mass concentration to spray for the biological prevention and control agent solution of 1-3g/L every 5-7d Tobacco cauline leaf is applied, is sprayed 2-3 times altogether;Mass concentration is used to pour for the biological prevention and control agent solution of 0.3-0.5g/L every 7-10d Tobacco root is filled, is poured 2-3 times altogether.
8. a kind of application method of the biological prevention and control agent containing wood vinegar as claimed in claim 1 or 2, which is characterized in that described Application method specifically: tobacco leaf picking is after sunning or baking, before entering warehouse storage, by the door and window in warehouse and wall gap It obturages, carries out suffocating treatment, usage amount 5-10g/m using the biological prevention and control agent3
9. a kind of application method of biological prevention and control agent containing wood vinegar according to claim 8, which is characterized in that described smoked The time for steaming processing is 24-48h.
CN201910230717.8A 2019-03-26 2019-03-26 A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application Pending CN110050799A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910230717.8A CN110050799A (en) 2019-03-26 2019-03-26 A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910230717.8A CN110050799A (en) 2019-03-26 2019-03-26 A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application

Publications (1)

Publication Number Publication Date
CN110050799A true CN110050799A (en) 2019-07-26

Family

ID=67317353

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910230717.8A Pending CN110050799A (en) 2019-03-26 2019-03-26 A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application

Country Status (1)

Country Link
CN (1) CN110050799A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110819354A (en) * 2019-10-18 2020-02-21 贵州鼎亿生物科技有限公司 Sweet wormwood herb withering-removing agent and preparation method thereof

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1802910A (en) * 2006-01-24 2006-07-19 浙江林学院 Agricultural chemicals preparation containing pyracetic acid
CN102310966A (en) * 2010-07-01 2012-01-11 郑州大学 Storage mildew-proof and moth-proof packaging hemp cloth and preparation method thereof
CN102604856A (en) * 2011-12-22 2012-07-25 中国农业科学院烟草研究所 Biological control serratia marcescens strain for general tobacco mosaic virus
CN102763693A (en) * 2011-05-04 2012-11-07 潘常光 Multifunctional high-efficiency plant regulator and its production method
CN104798824A (en) * 2015-02-15 2015-07-29 抚顺市世纪东方生物质综合技术开发有限公司 Botanical fungicide as well as preparation, use and storage methods thereof
CN106754557A (en) * 2017-01-25 2017-05-31 贵州省烟草公司贵阳市公司 Bacillus subtilis YBM 4 and its application in preventing and treating tobacco black shank and growth promotion
CN106900765A (en) * 2017-02-20 2017-06-30 贵州嘉利达科技有限公司 A kind of inert dust expelling parasite agent prescription for tobacco storage and preparation method thereof
CN107960103A (en) * 2014-12-29 2018-04-24 Fmc有限公司 For promoting plant growth and treating the microbial composite and application method of plant disease
KR20180060146A (en) * 2016-11-28 2018-06-07 김공수 Method for producing eco-friendly composition for controlling plant diseases using microemulsion and microcapsule of plant extract
CN108371192A (en) * 2018-02-23 2018-08-07 荆丽杰 A kind of Traditional Chinese medicine disinfectant and preparation method thereof for medical waste coagulation

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1802910A (en) * 2006-01-24 2006-07-19 浙江林学院 Agricultural chemicals preparation containing pyracetic acid
CN102310966A (en) * 2010-07-01 2012-01-11 郑州大学 Storage mildew-proof and moth-proof packaging hemp cloth and preparation method thereof
CN102763693A (en) * 2011-05-04 2012-11-07 潘常光 Multifunctional high-efficiency plant regulator and its production method
CN102604856A (en) * 2011-12-22 2012-07-25 中国农业科学院烟草研究所 Biological control serratia marcescens strain for general tobacco mosaic virus
CN107960103A (en) * 2014-12-29 2018-04-24 Fmc有限公司 For promoting plant growth and treating the microbial composite and application method of plant disease
CN104798824A (en) * 2015-02-15 2015-07-29 抚顺市世纪东方生物质综合技术开发有限公司 Botanical fungicide as well as preparation, use and storage methods thereof
KR20180060146A (en) * 2016-11-28 2018-06-07 김공수 Method for producing eco-friendly composition for controlling plant diseases using microemulsion and microcapsule of plant extract
CN106754557A (en) * 2017-01-25 2017-05-31 贵州省烟草公司贵阳市公司 Bacillus subtilis YBM 4 and its application in preventing and treating tobacco black shank and growth promotion
CN106900765A (en) * 2017-02-20 2017-06-30 贵州嘉利达科技有限公司 A kind of inert dust expelling parasite agent prescription for tobacco storage and preparation method thereof
CN108371192A (en) * 2018-02-23 2018-08-07 荆丽杰 A kind of Traditional Chinese medicine disinfectant and preparation method thereof for medical waste coagulation

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
《上海博物馆集刊》编辑委员会编: "《上海博物馆集刊 第三期》", 30 April 1986, 上海古籍出版社 *
中国土农药志编辑委员会: "《中国土农药志》", 31 May 1959, 科学出版社 *
中国食品添加剂和配料协会编著: "《食品添加剂手册》", 30 September 2012, 中国轻工业出版社 *
国家烟草专卖局、全国烟草标准化技术委员会: "《烟草技术标准汇编(三)》", 30 November 2001, 中国标准出版社 *
尼克: "《香草好玩又好种》", 31 July 2014, 海天出版社 *
谷继成 等: "《桃标准化生产技术》", 31 January 2008, 金盾出版社 *
邹莉: "《资源微生物》", 31 July 2005, 东北林业大学出版社 *
郑武城: "《光学化工辅料》", 30 April 1985, 测绘出版社 *
马国胜: "《烟草疫霉菌及其病害生态治理研究》", 31 December 2015, 苏州大学出版社 *
黄细喜 等: "《自然农法 绿色哲学的理论与实践》", 31 December 1987, 黑龙江人民出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110819354A (en) * 2019-10-18 2020-02-21 贵州鼎亿生物科技有限公司 Sweet wormwood herb withering-removing agent and preparation method thereof

Similar Documents

Publication Publication Date Title
CN106615085A (en) Microorganism and plant derived composite fresh keeping agent for fruits and vegetables
CN103535389B (en) Plant source fruit tree insecticide
CN103548895A (en) Botanical vegetable insecticide
CN107969443A (en) A kind of preparation method for preventing Citrus Huanglongbing pathogen medicament
CN107494080A (en) A kind of method that coccid is killed in the potted plant cultivating process of succulent
CN103385283A (en) Preparation method for antiseptic insect-proof antistaling agent used for bacon
CN106490028A (en) A kind of agricultural chemicals of preventing and treating elimination of underground destructive pests and preparation method thereof
CN101856027A (en) Pesticide preparation for controlling diamond back moth and other vegetable insect pests
CN110050799A (en) A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application
CN105123700B (en) A kind of insecticidal bactericide containing Thymol and arecaline and preparation method thereof
CN112136817A (en) A drying injection for preventing and controlling pine wood nematode disease and Monochamus alternatus
CN108812707B (en) Thrips killing plant essential oil composition, preparation and application thereof
CN107188736A (en) A kind of special culture medium of watermelon seedlings nutritive cube and preparation method thereof
CN108719345B (en) Origanum vulgaris essential oil preparation, preparation method and application thereof
CN105884544A (en) Brassica juncea fertilizer additive and preparation method thereof
CN105284963B (en) Cock skin fruit tree special plant fungicide and its production method
CN102176831A (en) A process for the production of a storage stable fungicidal extract of glycyrrhiza glabra for the control of phytopathogenic fungi and other plant diseases
CN103875714A (en) Bacillus thuringiensis.spinosyns.trisiloxane polyoxyethylene ether composition and use of bacillus thuringiensis.spinosyns.trisiloxane polyoxyethylene ether composition for preventing and controlling rice boring insects
Swaminathan et al. Anti-feedant activity of some biopesticides on Henosepilachna vigintioctopunctata (F.)(Coleoptera: Coccinellidae)
CN106490014A (en) A kind of plant compound pesticide containing ricidine. and preparation method thereof
CN106134698A (en) A kind of pollution-free grape planting method
CN102498984B (en) Method for preventing and treating plutella xylostella by applying lemongrass essential oil
KR20120060585A (en) Composition for Controling Lycorma delicatula, the Methods of Preparing it and the Method of Controling Lycorma delicatula Using it
CN105494349A (en) Method for preventing and curing cotinus-coggygria fusarium wilt
CN104892155A (en) Tip killing agent with synergistic and bactericidal effects

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20190726

RJ01 Rejection of invention patent application after publication