CN110050799A - A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application - Google Patents
A kind of biological prevention and control agent containing wood vinegar and its application, preparation and application Download PDFInfo
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- CN110050799A CN110050799A CN201910230717.8A CN201910230717A CN110050799A CN 110050799 A CN110050799 A CN 110050799A CN 201910230717 A CN201910230717 A CN 201910230717A CN 110050799 A CN110050799 A CN 110050799A
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- control agent
- wood vinegar
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- powder
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
- A01N61/02—Mineral oils; Tar oils; Tar; Distillates, extracts or conversion products thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B1/00—Preparation of tobacco on the plantation
- A24B1/02—Arrangements in barns for preparatory treatment of the tobacco, e.g. with devices for drying
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
Abstract
The present invention relates to biological prevention and control agent technical fields, and in particular to a kind of biological prevention and control agent containing wood vinegar and its application, preparation and application.Biological prevention and control agent of the invention includes following component: wood vinegar, zanthoxylum powder, Paprika, ginger powder, Herba Menthae powder, thyme powder, sweet wormwood powder, bay leaf powder, madder powder and complex micro organism fungicide, and the complex micro organism fungicide includes serratia marcescens, bacillus subtilis, bacillus amyloliquefaciens, Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.The biological agent is prepared using advanced method, the prevention and treatment gone mouldy applied to tobacco black shank and tobacco leaf storage phase, it is used in the different phase of tobacco growing and storage period by distinct methods, it can be effectively reduced the disease incidence and tobacco mildew rate of tobacco black shank, chemical prevention is reduced to the pollution of environment and tobacco leaf and the loss of tobacco leaf, and biological prevention and control agent dosage of the invention is less, the degradable noresidue of ingredient, it is environmentally protective, it is not likely to produce drug resistance.
Description
Technical field
The present invention relates to biological prevention and control agent technical fields, and in particular to a kind of biological prevention and control agent containing wood vinegar and its application,
Preparation and application.
Background technique
Tobacco black shank is one of the important disease occurred during tobacco fertility, can be occurred with field period in seedling stage, main
Cause harm basal part of stem, root and blade, each main production cigarette district in China has different degrees of generation, endangers heavier.Plant disease incidence
Less serious case 5-12%, serious plot disease incidence reaches 30% or more, or even causes to have no harvest, in recent years due to some local continuous cropping tobacco field faces
Product expands, and it is spring cigarette that the continuous cropping time limit, which extends or change winter cigarette, and the disease incidence of tobacco black shank has the gesture of obvious rising.In addition to this,
The natural alcoholization storage period is long after tobacco leaf harvest, since tobacco leaf hygroscopicity is strong, and containing needed for multiple-microorganism growth in tobacco leaf
Nutriment, tobacco leaf easily breeds various microorganisms and goes mouldy in storage.
Tobacco black shank relies primarily on chemical prevention at present, but increases the resistance of pathogen gradually using chemical agent year after year
By force, it needs that dosage is continuously improved, and preventive effect tends not to pollution that is satisfactory, but also will cause environment and agricultural product.
And the sterilization of the chemical anti mildew agents such as common benzoic acid, sodium benzoate, sorbic acid and propionic acid, antibacterial effect in tobacco industry at present
After power is smaller, and dosage is larger, and pipe tobacco is added in these chemical agents, when cigarette is burnt and sucked because Pintsch process can be generated to people
The harmful gas of body.
Wood vinegar be the biomass such as timber in distillation apparatus after destructive distillation derived steam gas mixture through condensing and separating
A kind of bronzing liquid obtained afterwards agriculturally has improvement soil, prevention and treatment pest and disease damage, promotes plant growth and is improving product
The effects of quality, but the effect that wood vinegar prevention and treatment tobacco black shank is administered alone during tobacco planting is poor, also has no wood
The vinegar liquid report mould proof in tobacco leaf storage phase energy.
Summary of the invention
Against the above technical problems, the object of the present invention is to provide a kind of biological prevention and control agent containing wood vinegar and its application,
Preparation and application reduces pollution of the chemical prevention to environment and tobacco leaf, and improve to reduce the disease incidence of tobacco black shank
Tobacco leaf storage phase mildew phenomena reduces tobacco mildew rate, reduces tobacco leaf loss.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 50-100 parts of wood vinegar, Chinese prickly ash
1-5 parts of powder, 1-5 parts of Paprika, 1-5 parts of ginger powder, 1-5 parts of Herba Menthae powder, 1-5 parts of thyme powder, 1-5 parts of sweet wormwood powder, basyleave
1-5 parts of powder, 1-5 parts of madder powder and 10-20 parts of complex micro organism fungicide, the complex micro organism fungicide includes Serratia
Bacterium, bacillus subtilis, bacillus amyloliquefaciens, Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
Further, in the complex micro organism fungicide include serratia marcescens 1-4 parts, 3-5 parts of bacillus subtilis,
1-2 parts of 3-5 parts of bacillus amyloliquefaciens, 2-4 parts of Rhodopseudomonas palustris and wine red finger sporangiocyst bacterium.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind
It is dry, it is pulverized and mixed with pulverizer, obtains mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
Further, 20 meshes are crossed after the mixed plant powder mixing in the step (1).
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco
The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match
The seed-soaking liquid that the biological prevention and control agent mass concentration is 0.5-1g/L is made, seed is placed in seed-soaking liquid and impregnates 30-60min, is taken out
It sows after natural drying.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: after tobacco transplant, often
It uses mass concentration to spray tobacco cauline leaf for the biological prevention and control agent solution of 1-3g/L every 5-7d, sprays 2-3 times altogether;Every 7-
10d uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.3-0.5g/L, pours 2-3 times altogether.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: tobacco leaf picking is through drying
Or after baking, before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, fumigated using the biological prevention and control agent
Processing, usage amount 5-10g/m3。
Further, the time of the suffocating treatment is 24-48h.
Compared with prior art, the invention has the following beneficial effects:
(1) wood vinegar soak extraction effective component is added after shining dry grinding in selection various plants, can improve itself and wood
The control efficiency that vinegar liquid goes mouldy to tobacco black shank and tobacco leaf storage phase, with clear water or ordinary organic solvents such as methanol, ethyl alcohol or
The soak extractions such as acetone are without this effect.The leaching efficiency that can improve effective component is impregnated after sieving again.Contain in Chinese prickly ash and hot red pepper
The plurality of active ingredients such as volatile oil, alkaloid, organic acid, flavonoids and cumarin contain curcumin, gingerol and ginger in ginger
The effective components such as essential oil, have the effect of Antimicrobial preservative, three be manured into soil in can also be to root-knot nematode, cutworm, mole cricket, gold
The subterranean pest-insects such as needle worm and grub play avoidance effect, reduce because subterranean pest-insect black shank pathogen caused by root of biting is invaded
Dye;Contain menthol and menthol in peppermint, thyme essential oil and Thymol are contained in thyme, contains sweet wormwood in sweet wormwood
Element, sitosterol and stigmasterol, basyleave contain there are many chromocor compound, contain rubican, munjistin, Physcion in madder
Equal ingredients can be formed after chelating to tobacco black shank pathogen after above a variety of different plant societys are extracted with wood vinegar
There is the ingredient of killing or inhibiting effect with the mould generated during storage cigarette, such as a variety of alkaloids have pathogen stronger
Permeability can directly kill pathogen, and alkaloid can also destroy the cell wall of pathogen and inhibit mycelia growth, make pathogen
It loses activity, reduces infection rate;Special composition in various plants can destroy the oxidation-reduction process of pathogen, reach sterilization and make
With;Wood vinegar soak after extraction also contains various trace elements, as contained in copper, zinc, calcium and sulphur and wood vinegar
More than 100 kinds of organic acids, phenols, ketones and alcohols substance, can promote tobacco growing, strengthen immunity and disease resistance, thus indirectly
Play antibacterial, disease-resistant effect.Wood vinegar and effective ingredients in plant can also accelerate beneficial microorganism breeding, with complex microorganism
Microbial inoculum compounding application can improve beneficial bacterium to the antagonistic effect of pathogen.
(2) tobacco seed is placed in the seed-soaking liquid that biological prevention and control agent mass concentration is 0.5-1g/L after impregnating to dry and is carried out again
Sowing can form one layer of defence film in the surface of the seed, and promote root system strong, prevent pathogen from invading from the surface of the seed or young root
Cause disease.Pathogen infection blade, petiole and stem can be prevented by spraying cauline leaf using the biological prevention and control agent after dilution in tobacco in seedling stage
Portion, moreover it is possible to tobacco plant chlorophyll content is improved, boosts metabolism and is accumulated with stem's lignin, improves stalk mechanical strength,
Reduce the infection probability of pathogen;Tobacco plant root is poured using the biological prevention and control agent after dilution, can be promoted in soil beneficial to micro-
The breeding of biology, the growth and communication process of antagonism pathogen.Tobacco leaf picking is stored after sunning or baking entering warehouse
Before, it is fumigated in warehouse using biological prevention and control agent, the effective component in wood vinegar and various plants can be made to infiltrate into closed
Storage space can be eliminated effectively and store interior hiding a variety of pathogens and mould, such as aspergillus, mould, Mucor and trichoderma are micro-
Biology prevents tobacco leaf from going mouldy in storage.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention,
Technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is the present invention one
Divide embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making
Every other embodiment obtained, shall fall within the protection scope of the present invention under the premise of creative work.
Wood vinegar used in following embodiment, Chinese prickly ash, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave, madder
Grass, serratia marcescens, bacillus subtilis, bacillus amyloliquefaciens, Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine are
It is commercially available.
Embodiment 1
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 50 parts of wood vinegar, zanthoxylum powder 1
Part, 3 parts of Paprika, 5 parts of ginger powder, 3 parts of Herba Menthae powder, 5 parts of thyme powder, 1 part of sweet wormwood powder, 3 parts of bay leaf powder, 1 part of madder powder
With 10 parts of complex micro organism fungicide, include in the complex micro organism fungicide 1 part of serratia marcescens, 3 parts of bacillus subtilis,
1 part of 3 parts of bacillus amyloliquefaciens, 2 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind
It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco
The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match
The seed-soaking liquid that the biological prevention and control agent mass concentration is 0.5g/L is made, seed is placed in seed-soaking liquid and impregnates 60min, nature is taken out and dries in the air
It is sowed after dry;After tobacco transplant, mass concentration is used to spray tobacco cauline leaf for the biological prevention and control agent solution of 3g/L every 5d,
It sprays altogether 3 times;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.3g/L every 8d, pours 2 times altogether;
Tobacco leaf picking before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, the life is used after sunning or baking
Anti- preparation carries out suffocating treatment, usage amount 5g/m3, the time of the suffocating treatment is for 24 hours.
Embodiment 2
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 75 parts of wood vinegar, zanthoxylum powder 3
Part, 5 parts of Paprika, 1 part of ginger powder, 5 parts of Herba Menthae powder, 1 part of thyme powder, 3 parts of sweet wormwood powder, 5 parts of bay leaf powder, 3 parts of madder powder
With 15 parts of complex micro organism fungicide, include in the complex micro organism fungicide 1 part of serratia marcescens, 4 parts of bacillus subtilis,
2 parts of 4 parts of bacillus amyloliquefaciens, 4 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind
It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco
The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match
The seed-soaking liquid that the biological prevention and control agent mass concentration is 0.75g/L is made, seed is placed in seed-soaking liquid and impregnates 45min, takes out nature
It is sowed after drying;After tobacco transplant, mass concentration is used to spray tobacco stem for the biological prevention and control agent solution of 2g/L every 6d
Leaf sprays 2 times altogether;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.4g/L every 10d, pours altogether
2 times;Tobacco leaf picking before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, institute is used after sunning or baking
It states biological prevention and control agent and carries out suffocating treatment, usage amount 7.5g/m3, the time of the suffocating treatment is 36h.
Embodiment 3
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 100 parts of wood vinegar, zanthoxylum powder 5
Part, 1 part of Paprika, 3 parts of ginger powder, 1 part of Herba Menthae powder, 3 parts of thyme powder, 5 parts of sweet wormwood powder, 1 part of bay leaf powder, 5 parts of madder powder
With 20 parts of complex micro organism fungicide, include in the complex micro organism fungicide 4 parts of serratia marcescens, 5 parts of bacillus subtilis,
2 parts of 5 parts of bacillus amyloliquefaciens, 4 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind
It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco
The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match
The seed-soaking liquid that the biological prevention and control agent mass concentration is 1g/L is made, seed is placed in seed-soaking liquid and impregnates 30min, takes out naturally dry
After sow;After tobacco transplant, mass concentration is used to spray tobacco cauline leaf for the biological prevention and control agent solution of 1g/L every 7d, altogether
It sprays 2 times;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.5g/L every 9d, pours 3 times altogether;Cigarette
Leaf harvests after sunning or baking, before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, the biological and ecological methods to prevent plant disease, pests, and erosion is used
Preparation carries out suffocating treatment, usage amount 10g/m3, the time of the suffocating treatment is 48h.
Embodiment 4
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 50 parts of wood vinegar, zanthoxylum powder 3
Part, 1 part of Paprika, 5 parts of ginger powder, 3 parts of Herba Menthae powder, 5 parts of thyme powder, 1 part of sweet wormwood powder, 1 part of bay leaf powder, 5 parts of madder powder
With 13 parts of complex micro organism fungicide, include in the complex micro organism fungicide 2 parts of serratia marcescens, 3 parts of bacillus subtilis,
1 part of 4 parts of bacillus amyloliquefaciens, 3 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind
It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco
The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match
The seed-soaking liquid that the biological prevention and control agent mass concentration is 0.5g/L is made, seed is placed in seed-soaking liquid and impregnates 60min, nature is taken out and dries in the air
It is sowed after dry;After tobacco transplant, mass concentration is used to spray tobacco cauline leaf for the biological prevention and control agent solution of 1g/L every 5d,
It sprays altogether 3 times;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.5g/L every 7d, pours 3 times altogether;
Tobacco leaf picking before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, the life is used after sunning or baking
Anti- preparation carries out suffocating treatment, usage amount 5g/m3, the time of the suffocating treatment is for 24 hours.
Embodiment 5
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 75 parts of wood vinegar, zanthoxylum powder 1
Part, 5 parts of Paprika, 3 parts of ginger powder, 3 parts of Herba Menthae powder, 1 part of thyme powder, 5 parts of sweet wormwood powder, 3 parts of bay leaf powder, 3 parts of madder powder
With 17 parts of complex micro organism fungicide, include in the complex micro organism fungicide 3 parts of serratia marcescens, 5 parts of bacillus subtilis,
2 parts of 4 parts of bacillus amyloliquefaciens, 3 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind
It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco
The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match
The seed-soaking liquid that the biological prevention and control agent mass concentration is 0.75g/L is made, seed is placed in seed-soaking liquid and impregnates 45min, takes out nature
It is sowed after drying;After tobacco transplant, mass concentration is used to spray tobacco stem for the biological prevention and control agent solution of 3g/L every 6d
Leaf sprays 3 times altogether;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.4g/L every 8d, pours 2 altogether
It is secondary;Tobacco leaf picking before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, institute is used after sunning or baking
It states biological prevention and control agent and carries out suffocating treatment, usage amount 7.5g/m3, the time of the suffocating treatment is 36h.
Embodiment 6
A kind of biological prevention and control agent containing wood vinegar, including following component in parts by weight: 100 parts of wood vinegar, zanthoxylum powder 5
Part, 3 parts of Paprika, 1 part of ginger powder, 1 part of Herba Menthae powder, 5 parts of thyme powder, 3 parts of sweet wormwood powder, 5 parts of bay leaf powder, 1 part of madder powder
With 13 parts of complex micro organism fungicide, include in the complex micro organism fungicide 2 parts of serratia marcescens, 4 parts of bacillus subtilis,
1 part of 3 parts of bacillus amyloliquefaciens, 3 parts of Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
A kind of preparation method of the biological prevention and control agent containing wood vinegar, comprising the following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried into wind
It is dry, 20 meshes are crossed after being pulverized and mixed with pulverizer, obtain mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
A kind of application of the biological prevention and control agent containing wood vinegar, the biological prevention and control agent containing wood vinegar are applied to the black shin of tobacco
The prevention and treatment of disease and tobacco leaf storage phase mildew.
A kind of application method of the biological prevention and control agent containing wood vinegar, the application method specifically: before tobacco sowing, match
The seed-soaking liquid that the biological prevention and control agent mass concentration is 1g/L is made, seed is placed in seed-soaking liquid and impregnates 30min, takes out naturally dry
After sow;After tobacco transplant, mass concentration is used to spray tobacco cauline leaf for the biological prevention and control agent solution of 2g/L every 7d, altogether
It sprays 2 times;It uses mass concentration to pour tobacco root for the biological prevention and control agent solution of 0.3g/L every 9d, pours 3 times altogether;Cigarette
Leaf harvests after sunning or baking, before entering warehouse storage, the door and window in warehouse and wall gap is obturaged, the biological and ecological methods to prevent plant disease, pests, and erosion is used
Preparation carries out suffocating treatment, usage amount 10g/m3, the time of the suffocating treatment is 48h.
Medicament is not used in comparative example 1.
Comparative example 2 prevents and treats tobacco black shank using 30% first frost hymexazol of high-tech, 600 times of liquid.
Comparative example 3 is gone mouldy using the sodium benzoate prevention and treatment tobacco leaf storage phase.
Comparative example 4 is the difference from embodiment 1 is that be used only wood vinegar.
Comparative example 5 impregnates the soak that plant component obtains the difference from embodiment 1 is that clear water is used only.
Comparative example 6 is the difference from embodiment 1 is that be used only complex micro organism fungicide.
Comparative example 7 is the difference from embodiment 1 is that biological prevention and control agent is only used in seed soaking.
Comparative example 8 is the difference from embodiment 1 is that biological prevention and control agent only uses after transplanting.
Comparative example 9 is the difference from embodiment 1 is that biological prevention and control agent is only used in storage period.
Balck shank disease incidence and tobacco mildew rate are investigated in tobacco growing and storage, as a result such as following table.
1 tobacco growing phase of table balck shank disease incidence and storage period tobacco mildew rate
From the data in table 1, it can be seen that preventing with the comparative example 1 of unused medicament and using 30% 600 times of liquid of first frost hymexazol of high-tech
The comparative example 2 for controlling tobacco black shank is compared, and the disease incidence of tobacco black shank is substantially reduced in embodiment 1-6, is averagely reduced
8.97% and 3.08%.The comparative example 3 of tobacco leaf storage phase mildew is prevented and treated with the comparative example 1 of unused medicament and using sodium benzoate
It compares, storage period, tobacco mildew rate also decreased significantly in embodiment 1-6, averagely reduce 1.298% and 0.664%.Explanation
Compared with conventional control method, using biological prevention and control agent of the invention to reduction tobacco black shank disease incidence and storage period tobacco mildew
Rate has obvious effect.
Compared with embodiment 1 and comparative example 2, plant component is impregnated using only the comparative example 4 of wood vinegar, using only clear water and is obtained
To soak comparative example 5 and using only the disease incidence of tobacco black shank in the comparative example 6 of complex micro organism fungicide it is all higher,
But lower than the comparative example 1 not being administered, illustrate that three in the biological prevention and control agent kinds of ingredient exclusive uses have certain prevention and treatment cigarette
The effect of careless balck shank, but effect is worse than general chemistry prevention and treatment, and the effect that complex micro organism fungicide is used alone in three kinds of ingredients
Fruit is best.Compared with embodiment 1 and comparative example 3, it is higher than embodiment using only tobacco mildew rate in a kind of comparative example 4-6 of ingredient
1 but it is lower than comparative example 3, illustrates that the effect for having certain reduction tobacco mildew rate is used alone in three in the biological prevention and control agent kinds of ingredients
Fruit, and plant component clear water soak effect therein is not so good as common preservatives, but singly applies wood vinegar and singly apply composite microbial
The effect of object microbial inoculum is superior to common preservatives.
Compared with Example 1, biological prevention and control agent is only used after transplanting using the comparative example 7 of biological prevention and control agent and only in seed soaking
Comparative example 8 in tobacco black shank disease incidence and storage period tobacco mildew rate it is higher, but lower than the comparative example that is not administered
1, illustrate that the biological prevention and control agent only also has certain reduction balck shank disease incidence and storage in the use of a certain period of tobacco growing phase
The effect of phase tobacco mildew rate.And only storage period using biological prevention and control agent 9 tobacco black shank disease incidence of comparative example be not administered
Comparative example 1 without significant difference, tobacco mildew rate is higher than embodiment 1 but is lower than other comparative examples, illustrates according to the invention make
The effect for reducing balck shank disease incidence and storage period tobacco mildew rate with method is best.
In conclusion a kind of biological prevention and control agent containing wood vinegar of the invention, in tobacco growing and the not same order of storage period
Section is used by distinct methods, can be effectively reduced the disease incidence and tobacco mildew rate of tobacco black shank, reduces chemical prevention to ring
The pollution of border and tobacco leaf and the loss of tobacco leaf, and biological prevention and control agent dosage of the invention is less, the degradable noresidue of ingredient, green ring
It protects, is not likely to produce drug resistance.
Claims (9)
1. a kind of biological prevention and control agent containing wood vinegar, which is characterized in that including following component in parts by weight: wood vinegar 50-
100 parts, 1-5 parts of zanthoxylum powder, 1-5 parts of Paprika, 1-5 parts of ginger powder, 1-5 parts of Herba Menthae powder, 1-5 parts of thyme powder, sweet wormwood powder 1-5
Part, 1-5 parts of bay leaf powder, 1-5 parts of madder powder and 10-20 parts of complex micro organism fungicide, the complex micro organism fungicide include viscous
Matter Serratieae, bacillus subtilis, bacillus amyloliquefaciens, Rhodopseudomonas palustris and the red finger sporangiocyst bacterium of wine.
2. a kind of biological prevention and control agent containing wood vinegar according to claim 1, which is characterized in that the composite microbial bacteria
It include serratia marcescens 1-4 parts, 3-5 parts of bacillus subtilis, 3-5 parts of bacillus amyloliquefaciens, the red false unit cell in marsh in agent
1-2 parts of 2-4 parts of bacterium and wine red finger sporangiocyst bacterium.
3. a kind of preparation method of the biological prevention and control agent containing wood vinegar as claimed in claim 1 or 2, which is characterized in that including
Following steps:
(1) Chinese prickly ash of the parts by weight, hot red pepper, ginger, peppermint, thyme, sweet wormwood, basyleave and madder are dried and is air-dried, used
Pulverizer is pulverized and mixed, and obtains mixed plant powder;
(2) the mixed plant powder is mixed with wood vinegar, impregnates 48h at room temperature, obtains wood vinegar soak;
(3) strainer filtering wood vinegar soak is used, the mixed plant powder after impregnating is removed, obtains impregnating wood vinegar;
(4) complex micro organism fungicide is added into the immersion wood vinegar to get the biological prevention and control agent is arrived.
4. a kind of preparation method of biological prevention and control agent containing wood vinegar according to claim 3, which is characterized in that the step
Suddenly 20 meshes are crossed after the mixed plant powder mixing in (1).
5. a kind of application of the biological prevention and control agent containing wood vinegar as claimed in claim 1 or 2, which is characterized in that described to contain
The prevention and treatment that the biological prevention and control agent of wood vinegar is applied to tobacco black shank and the tobacco leaf storage phase goes mouldy.
6. a kind of application method of the biological prevention and control agent containing wood vinegar as claimed in claim 1 or 2, which is characterized in that described
Application method specifically: before tobacco sowing, the seed-soaking liquid that the biological prevention and control agent mass concentration is 0.5-1g/L is prepared, by seed
It is placed in seed-soaking liquid and impregnates 30-60min, taking-up is sowed after natural drying.
7. a kind of application method of the biological prevention and control agent containing wood vinegar as claimed in claim 1 or 2, which is characterized in that described
Application method specifically: after tobacco transplant, use mass concentration to spray for the biological prevention and control agent solution of 1-3g/L every 5-7d
Tobacco cauline leaf is applied, is sprayed 2-3 times altogether;Mass concentration is used to pour for the biological prevention and control agent solution of 0.3-0.5g/L every 7-10d
Tobacco root is filled, is poured 2-3 times altogether.
8. a kind of application method of the biological prevention and control agent containing wood vinegar as claimed in claim 1 or 2, which is characterized in that described
Application method specifically: tobacco leaf picking is after sunning or baking, before entering warehouse storage, by the door and window in warehouse and wall gap
It obturages, carries out suffocating treatment, usage amount 5-10g/m using the biological prevention and control agent3。
9. a kind of application method of biological prevention and control agent containing wood vinegar according to claim 8, which is characterized in that described smoked
The time for steaming processing is 24-48h.
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