CN108719345B - Origanum vulgaris essential oil preparation, preparation method and application thereof - Google Patents
Origanum vulgaris essential oil preparation, preparation method and application thereof Download PDFInfo
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- CN108719345B CN108719345B CN201810650686.7A CN201810650686A CN108719345B CN 108719345 B CN108719345 B CN 108719345B CN 201810650686 A CN201810650686 A CN 201810650686A CN 108719345 B CN108719345 B CN 108719345B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Natural Medicines & Medicinal Plants (AREA)
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Abstract
The invention discloses an origanum vulgaris essential oil preparation, a preparation method and application thereof, wherein the origanum vulgaris essential oil is used for preparing a medicament for preventing and treating thysanoptera pests on crops; an origanum essential oil preparation comprises an active ingredient of origanum essential oil, wherein the content of the origanum essential oil in the preparation is 10-20% by mass percent. The pesticide containing the oregano essential oil can prevent and treat various thrips, has sufficient raw materials, easy processing and low cost, is not easy to generate drug resistance, can improve the capability of a crop immune system, and is a pollution-free botanical thrip prevention and control agent which is safe to human beings and livestock and has no pollution to the environment.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to an origanum vulgaris essential oil preparation, a preparation method and application thereof.
Background
Thrips is a general term for insects of the thrips (thysannoptera) family (Thripida), and is small in size, highly hiding, and fast in reproduction. Thrips is mainly harmful to various grains, fruits, vegetables, flowers, gardening and the like. In recent years, thrips has been increasingly developed year by year, and has become one of the world-wide important agricultural and forestry pests, causing great economic loss to agricultural production practices.
At present, the prevention and treatment of thrips domestically and abroad mainly depend on chemical agents, however, the problem of drug resistance is becoming more serious and the pressure on the environment is increased. Therefore, it is necessary to develop a new thrips prevention and control agent, and plant-derived pesticides become a hotspot for research and development and application of new pesticides due to the advantages of environmental friendliness, safety to non-target organisms, difficulty in generating drug resistance and the like.
Disclosure of Invention
The first purpose of the invention is to provide the application of oregano essential oil in preparing the medicine for preventing and controlling the thysanoptera pests on crops, and the prevention and control effect on the pests is good.
The second purpose of the invention is to provide an origanum vulgaris essential oil preparation which has the characteristics of high efficiency, no residue, safety to non-target organisms, difficult generation of drug resistance, environmental friendliness and the like.
The third purpose of the invention is to provide a preparation method of origanum vulgare essential oil, which has the advantages of easily obtained raw materials, simple processing, low requirement on equipment and high extraction efficiency of effective components
The invention is realized by the following technical scheme:
according to a first object of the present invention, the use of oregano essential oil for the preparation of a medicament for controlling pests of the order thysanoptera on crops.
Optionally, the oregano essential oil is an essential oil product obtained by water extraction and organic solvent distillation of oregano whole plants.
Optionally, the crops comprise grains, fruits and vegetables, flowers and/or horticultural plants.
Optionally, the pests of the order thysanoptera include lothrips zeae, thrips occidentalis and/or thrips tabaci.
According to the second object of the invention, the origanum essential oil preparation comprises the active ingredient of the origanum essential oil, and the content of the origanum essential oil in the preparation is 5-30% by mass percent.
The origanum essential oil preparation can also comprise an active ingredient of the origanum essential oil, and the content of the origanum essential oil in the preparation is 10-20% by mass percent.
Optionally, the formulation is in a dosage form selected from the group consisting of a hot fogging concentrate and a soluble liquor.
Optionally, the auxiliary agent used in the hot fogging concentrate or the soluble liquid formulation is at least one of an emulsifier and a solvent;
the emulsifier is one or more of agricultural milk 0201-B, agricultural milk 0203-B, agricultural milk 1601, agricultural milk 1602 and agricultural milk 500;
the solvent is one or more selected from N, N-dimethylformamide, methylnaphthalene, turpentine, soybean oil, dimethyl sulfoxide, methylnaphthalene and anhydrous ethanol.
In accordance with a third object of the present invention, a method for preparing a oregano essential oil formulation, comprising: extracting 300g dried origanum vulgaris in 400ml water under ultrasonic wave for 60min, distilling with steam distillation device for 2h to obtain distillate, distilling the distillate with dichloromethane to obtain fraction, removing water from the fraction to obtain origanum vulgaris essential oil, and making into preparation.
The oregano essential oil preparation is applied to the pesticide for preventing and controlling the pests of thysanoptera on crops.
Compared with the prior art, the invention has the following advantages:
the pesticide containing the oregano essential oil has obvious effect of preventing and treating thrips, is not easy to generate drug resistance, and can play a role in sterilizing and improving the capability of a crop immune system.
Detailed Description
Origanum vulgare L is a labiate plant, the whole plant extract of the Origanum vulgare L contains more than 30 antibacterial compounds, is a potential natural antibiotic and a drug growth promoter, has important significance in the aspects of livestock feed, feed antibiotics, immunity and the like, and is found to have good insecticidal effect after relevant literature search, while no research report is made on the prevention and treatment of thrips.
The preparation of the oregano essential oil used in the present invention comprises: weighing 300g of dry origanum vulgaris whole plant, putting into a flask, adding 400ml of water, extracting for 60min under ultrasonic wave, distilling for 2h by using a steam distillation device, adding a certain amount of dichloromethane into distillate, distilling by using a rotary evaporator, adding a proper amount of anhydrous sodium sulfate to absorb water, and filtering to obtain the origanum vulgaris essential oil. Then, the origanum essential oil is prepared into hot fogging concentrate or soluble liquid.
Oregano whole grass is fresh, dried and purchased from shochu natural products, inc.
In order to improve the stability of the pesticide containing the oregano essential oil, the oregano essential oil is prepared into a hot fogging concentrate or a soluble liquid concentrate for use, and an auxiliary agent used in the hot fogging concentrate or the soluble liquid concentrate is at least one of an emulsifier and a solvent; the emulsifier is one or more of agricultural milk 0201-B, agricultural milk 0203-B, agricultural milk 1601, agricultural milk 1602 and agricultural milk 500; the solvent is one or more selected from N, N-dimethylformamide, methylnaphthalene, turpentine, soybean oil, dimethyl sulfoxide, methylnaphthalene and anhydrous ethanol.
The main technical indexes of the soluble liquid of the invention are as follows:
the hot fogging concentrate of the invention has the main technical indexes that:
the preparation containing oregano essential oil can be used for preventing and treating maize yellow and aphyllosis, western flower thrips and tobacco thrips on grains, fruits, vegetables, flowers, gardening and the like.
The following examples are given for the detailed implementation and specific operation of the present invention, but the scope of the present invention is not limited to the following examples.
Indoor bioassay research:
the indoor bioassay of the oregano essential oil adopts a triangular flask fumigation method. Before the test, the oregano essential oil is diluted into different concentration gradients by acetone and refrigerated for standby. The specific method comprises the following steps: 20 healthy and tidy test insects are inoculated into a triangular flask (V is 60mL), then a filter paper strip (3 x 1cm) is fixed on an eraser plug, 1 mu L of essential oil solution is dripped on the filter paper strip by using a micro-sample injector, the eraser plug is quickly plugged, the paper strip is just hung at 1/3 in the center of the flask, the flask is placed in an intelligent incubator (L: D is 16: 8, T is 25 +/-1 ℃, RH is 60 +/-10%), after 6h, the plug with the filter paper is pulled off, the flask opening is sealed by a preservative film, after 24h, the fumigation condition is observed, and the death rate is calculated and corrected by using an Abbott formula. The death criteria of the maize yellow foolish thrips are: the body of the insect was touched with a writing brush under the dissecting mirror, and the appendage and head and abdomen were not responsive. Each treatment was repeated three times, setting acetone control.
Corrected mortality (%) — treatment mortality vs control mortality)/(1 vs control mortality) × 100%.
Indoor bioassay results of oregano essential oil:
TABLE 1 results of indoor bioassay of oregano essential oil on thrips
The essential oil of oregano is a potential natural antibiotic and a drug growth promoter, has important significance in the aspects of livestock and poultry feed, feed antibiotic, immunity and the like, and is found to have good insecticidal effect after relevant literature retrieval, the inventor finds that the essential oil of oregano has good fumigating and killing effect on thrips through indoor bioassay research, and the experimental results in table 1 show that the correction death rate of various thrips including maize yellow thrips, western flower thrips and tobacco thrips is over 96 percent, and the effect is good.
Based on the above, the inventors further studied the oregano essential oil to prepare a hot fogging concentrate and a soluble liquid concentrate, respectively.
The first embodiment is as follows: preparation and application of 10% oregano essential oil hot fogging concentrate
10% oregano essential oil hot fogging concentrate by weight: origanum essential oil: 10%, Nongru 0201-B: 5% -35%, N, N-dimethylformamide: 5% -35%, turpentine oil: 5-20 percent, and the balance being made up to 100 percent by methylnaphthalene.
Example two: preparation and application of 15% origanum essential oil hot fogging concentrate
15% oregano essential oil hot fogging concentrate by weight: origanum essential oil: 15%, Nongru 0201-B: 5% -35%, N-dimethylformamide: 5% -35%, soybean oil: 5-20 percent, and the balance being made up to 100 percent by methylnaphthalene.
Example three: preparation and application of 20% oregano essential oil hot fogging concentrate
20% oregano essential oil hot fogging concentrate by weight: origanum essential oil: 20%, Nongru 0203-B: 5% -35%, dimethyl sulfoxide: 5% -35%, turpentine oil: 5 to 20 percent, and the balance of the total amount is up to 100 percent of methylnaphthalene.
The field pesticide effect test of the hot fogging concentrate adopts a plot test:
the cell test is random arrangement, repeated for 3 times, and the cell area is 1m2. The number of thrips leaves is 10 pieces per cell, the population base number is investigated before the drug is taken, and the number of thrips is investigated 1, 3 and 7 days after the drug is taken. The application adopts a hot fogging machine to apply, the hot fogging agent of the oregano essential oil is pressed into a fuming pipe by the aid of the hot fogging machine, and is mixed with high-temperature and high-speed hot air to be sprayed into the atmosphere to form smog, the smog is diffused and diffused in the field, and the pesticide enters the body of pests, so that the prevention and control effects are achieved. And (4) recording the insect quantity before and after the treatment and application of each medicament, calculating the control effect of each medicament treatment, and calculating the correction control effect.
The calculation formula is as follows:
the control effect is 1- (number of live insects before drug application in control area)
Number of live insects after drug application in the x treatment area)/(number of live insects after drug application in the control area
Number of live insects before application in the multiplied treatment area) multiplied by 100%
The test results are shown below:
TABLE 2 medicinal effect test of origanum oil hot fogging concentrate for preventing and treating thrips
As can be seen from the table 2, the oregano essential oil hot fogging concentrate has good field control effect on three types of thrips, has a long control period, and has no adverse effect on target crops in the test drug application range.
Example four: preparation and application of 10% soluble origanum essential oil liquid
The weight percentage of the 10% oregano essential oil soluble liquid is as follows: origanum essential oil: 10%, farm milk-1601: 5% -15%, farm milk-500: 1 to 5 percent, and the balance of the total weight is supplemented to 100 percent by absolute ethyl alcohol.
Example five: preparation and application of 15% soluble origanum essential oil liquid
15 percent of oregano essential oil soluble liquid by weight percentage: origanum essential oil: 15%, agricultural milk-1602: 5% -10%, farm milk-500: 1 to 5 percent, and the balance of the total weight is supplemented to 100 percent by absolute ethyl alcohol.
Example six: preparation and application of 20% soluble origanum essential oil liquid
20 percent of oregano essential oil soluble liquid by weight percentage: origanum essential oil: 20%, agricultural milk-1602: 5% -15%, farm milk-500: 1 to 5 percent, and the balance of the total weight is supplemented to 100 percent by absolute ethyl alcohol.
The test results are shown below:
the field efficacy test of the soluble liquid adopts a plot test:
the cell test is random arrangement, repeated for 3 times, and the cell area is 1m2. The number of thrips leaves is 10 pieces per cell, the population base number is investigated before the drug is taken, and the number of thrips is investigated 1, 3 and 7 days after the drug is taken. The pesticide is applied after being diluted by corresponding times by a sprayer, so that the pesticide enters the body of pests, and the prevention and treatment effect is achieved. And (4) recording the insect quantity before and after the treatment and application of each medicament, calculating the control effect of each medicament treatment, and calculating the correction control effect.
The calculation formula is as follows:
the control effect is 1- (number of live insects before drug application in control area)
Number of live insects after drug application in the x treatment area)/(number of live insects after drug application in the control area
Number of live insects before application in the multiplied treatment area) multiplied by 100%
TABLE 3 medicinal effect test of soluble origanum oil liquid for preventing and treating thrips
As can be seen from the table 3, the soluble oregano essential oil solution has good field control effect on three types of thrips, has long control period and has no adverse effect on target crops in the test drug application range.
Claims (2)
1. The application of the oregano essential oil in preparing a preparation for controlling pests of thysanoptera on crops is characterized in that the active component of the preparation is the oregano essential oil, and the content of the oregano essential oil in the preparation is 10-20% by mass percent;
the oregano essential oil is an essential oil product obtained by water extraction and organic solvent distillation of oregano whole plants;
the crops comprise grains, fruits and vegetables, flowers and/or horticultural plants;
the pests of the Thysanoptera comprise maize yellow thrips, western flower thrips and/or tobacco thrips;
extracting 300g of dried origanum vulgaris in 400ml of water under ultrasonic wave for 60min, distilling for 2h by using a steam distillation device to obtain distillate, adding a certain amount of dichloromethane into the distillate, distilling by using a rotary evaporator, adding a proper amount of anhydrous sodium sulfate to absorb water, filtering to obtain origanum essential oil, and preparing the origanum essential oil into a preparation.
2. Use of oregano essential oil in the preparation of a formulation for controlling pests of the order thysanoptera on a crop as claimed in claim 1, wherein the formulation is in a dosage form selected from the group consisting of a hot fogging concentrate and a soluble liquor;
the auxiliary agent used in the hot fogging concentrate or the soluble liquid formulation is at least one of an emulsifier and a solvent;
the emulsifier is one or more of agricultural milk 0201-B, agricultural milk 0203-B, agricultural milk 1601, agricultural milk 1602 and agricultural milk 500;
the solvent is one or more selected from N, N-dimethylformamide, turpentine, soybean oil, dimethyl sulfoxide, methylnaphthalene and anhydrous ethanol.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711447431.2 | 2017-12-27 | ||
| CN2017114474312 | 2017-12-27 |
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| CN108719345B true CN108719345B (en) | 2020-12-22 |
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| CN201810320438.6A Withdrawn CN108739876A (en) | 2017-12-27 | 2017-12-27 | A kind of Herba Origani Vulgaris quintessence oil preparation, preparation method and applications |
| CN201810650686.7A Active CN108719345B (en) | 2017-12-27 | 2018-06-22 | Origanum vulgaris essential oil preparation, preparation method and application thereof |
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| CN109601559B (en) * | 2019-01-07 | 2021-01-15 | 贵州省烟草公司遵义市公司 | Wild chrysanthemum essential oil for preventing and treating phytophthora and application thereof |
| CN110226682A (en) * | 2019-07-10 | 2019-09-13 | 大连民族大学 | It is a kind of for preventing and treating the fodder compound and preparation method thereof of pig salmonella |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1469710A (en) * | 2000-03-17 | 2004-01-21 | �Ƶ��ɹ�˾ | Plant acaricidal composition and method for using the same composition |
| CN1905796A (en) * | 2003-12-02 | 2007-01-31 | 植物冲击股份有限公司 | insecticide composition |
| CN103974621A (en) * | 2011-10-04 | 2014-08-06 | 0903608B.C.有限公司 | Pest control formulations and methods of making and using same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20030060379A1 (en) * | 2000-05-26 | 2003-03-27 | The Procter & Gamble Company | Pesticides |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1469710A (en) * | 2000-03-17 | 2004-01-21 | �Ƶ��ɹ�˾ | Plant acaricidal composition and method for using the same composition |
| CN1905796A (en) * | 2003-12-02 | 2007-01-31 | 植物冲击股份有限公司 | insecticide composition |
| CN103974621A (en) * | 2011-10-04 | 2014-08-06 | 0903608B.C.有限公司 | Pest control formulations and methods of making and using same |
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| CN108739876A (en) | 2018-11-06 |
| CN108719345A (en) | 2018-11-02 |
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Effective date of registration: 20210201 Address after: 712100 Shaanxi Province, Xi'an city Yangling District Tai Road No. 3 demonstration Patentee after: NORTHWEST A & F University Address before: 712100 Shaanxi Province, Xi'an city Yangling District Tai Road No. 3 demonstration Patentee before: RESEARCH & DEVELOPMENT CENTER OF BIORATIONAL PESTICIDE YANGLING NONGKEDA |