KR102094916B1 - Insecticide composition for Dermanyssus gallinae, and the preparation method thereof - Google Patents

Insecticide composition for Dermanyssus gallinae, and the preparation method thereof Download PDF

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KR102094916B1
KR102094916B1 KR1020170165196A KR20170165196A KR102094916B1 KR 102094916 B1 KR102094916 B1 KR 102094916B1 KR 1020170165196 A KR1020170165196 A KR 1020170165196A KR 20170165196 A KR20170165196 A KR 20170165196A KR 102094916 B1 KR102094916 B1 KR 102094916B1
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extract
pesticide composition
agent
chicken
active ingredient
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KR20190065730A (en
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조명행
하성섭
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조명행
하성섭
(주)지웰바이오
주식회사 패러다임파트너스
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Priority to PCT/KR2018/015072 priority patent/WO2019112254A2/en
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Abstract

본 발명은 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분, 침투이행제 및 부형제를 포함하는 살충제 조성물, 상기 살충제 조성물의 제조방법 및 닭진드기 살충방법에 관한 것이다. 본 발명에 따른 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분, 침투이행제 및 부형제를 포함하는 살충제 조성물은 닭진드기에 대한 살충효과가 우수하다. 따라서 이를 주기적으로 사용할 경우, 닭 진드기의 분포를 감소시켜 가축의 스트레스를 감소시키고 닭 진드기에 의해 매개되는 타 병원체의 감염을 차단할 수 있다. 특히, 본 발명의 식물 추출물은 식물체에서 추출한 물질이므로 친환경적으로 해충방제를 할 수 있는 장점이 있고, 기존의 화학적으로 합성된 살충제와 다른 작용기작을 가지고 있어 저항성 문제도 발생하지 않아 유용하게 사용될 수 있다.The present invention is a pesticide composition comprising an active ingredient for insecticide, a penetration migrating agent, and an excipient containing derris elliptica extract, paraffin, and citric acid as an active ingredient, a method for preparing the pesticide composition, and chicken mite pesticide It's about how. The pesticide composition comprising the active ingredient for insecticidal, dermal transfer agents and excipients comprising derris elliptica extract, paraffin and citric anhydride as an active ingredient according to the present invention has excellent insecticidal effect against chicken mites. . Therefore, when it is used periodically, it is possible to reduce the distribution of chicken mites, thereby reducing the stress of livestock and blocking infection of other pathogens mediated by chicken mites. Particularly, since the plant extract of the present invention is a material extracted from a plant, it has an advantage of environmentally friendly pest control, and has a mechanism of action different from that of a conventional chemically synthesized insecticide, so that it does not cause resistance problems and can be usefully used. .

Description

닭진드기 살충제 조성물 및 이의 제조방법{Insecticide composition for Dermanyssus gallinae, and the preparation method thereof}Insecticide composition for dermanyssus gallinae, and the preparation method thereof

본 발명은 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분, 침투이행제 및 부형제를 포함하는 살충제 조성물, 상기 살충제 조성물의 제조방법 및 닭진드기 살충방법에 관한 것이다.The present invention is an insecticidal composition comprising an active ingredient for insecticide, a penetration agent and an excipient containing derris elliptica extract, paraffin, and citric anhydride as active ingredients, a method for preparing the pesticide composition, and chicken mite pesticide It's about how.

닭진드기(응애, 와구모)는 거미강 진드기목에 해당하고, 국내뿐 아니라 전세계적으로 분포하고 있다. 닭진드기는 낮에는 가축의 축사나 기구에 기생하나, 야간에 닭에 이행하여 흡혈하는 습성을 가지고 있는 것으로 알려져 있다. 이러한 습성은 가축 질병원체의 전파와 연관성이 크며, 특히 산란을 하는 산란계에 있어서 닭진드기의 흡혈로 인한 산란율이 저하, 빈혈유발, 폐사 등을 발생시켜 그 피해가 널리 알려져 있다.Chicken mites (mite, wagumo) are arachnid mites, and are distributed worldwide as well as domestically. It is known that chicken mites parasitize livestock stalls and implements during the day, but have the habit of migrating to chickens at night. These habits are highly related to the spread of disease agents in livestock, and in particular, in spawning systems where spawning occurs, the spawning rate due to bloodsucking of chicken mites causes anemia, mortality, and the like.

닭진드기는 사료, 계사내 갈라진 틈, 캐비지 등에 알을 낳아 서식하며, 주로 자충과 암컷이 주로 흡혈하며, 수컷은 거의 흡혈을 하지 않고, 유충의 경우 흡혈을 하지 않는다. 흡혈 시간은 통상 30분~2시간정도 짧지만, 흡혈을 하지 않고도, 12개월 이상 생존함으로서 방제가 매우 어려운 해충이다. Chicken mites live by laying eggs on feeds, cracks in cages, and in cages. Mostly, female and female suffocates mainly, and males rarely bleed, and larvae do not. Vampire time is usually about 30 minutes to 2 hours short, but it is a very difficult pest to control by surviving for 12 months or more without blood sucking.

이러한 피해를 구제하기 위해 기존 카바메이트계, 유기인계, 유기염소계 살충제 성분으로 이루어진 농약 및 동물용 살충제 등을 현재까지 사용하여 왔으나, 상기와 같은 화학성분으로 된 살충제는 닭에게 극심한 자극을 유발시키고 증체량 감소, 산란율 저하를 야기하는 것으로 알려졌다. 또한 최근 화학성분의 살충제를 처리한 산란계의 계란에서 피프로닐 및 비페트린이 검출되었는데, 상기 피프로닐은 다량 섭취 시 인간에 있어 간, 갑상샘, 신장 등의 기능에 이상을 일으킬 수 있는 성분이며, 비페트린은 발암물질로 알려져 상당한 사회적 이슈를 야기시키고 있다. 아울러, 고독성의 농약 위주의 화학물 살포로 인해 닭진드기가 상당한 내성을 갖게 되는 문제가 발생하고 있다. 이에, 화학약품 처리로 인해 발생하게 되는 상기과 같은 문제를 야기하지 않는 천연 성분을 유효성분으로 포함하는 살충제에 관한 관심이 그 어느 때보다 높아지고 있으며, 닭 생태에 맞는 적절한 방제법을 찾기 위해 식물체 및 식물체 유래 정유에 근간하여 속효성과 살충력이 우수하면서 독성이 없어 기존의 화학적 살충제를 대체할 수 있는 천연유래 살충제의 개발이 요구되고 있는 실정이다Conventional carbamate-based, organophosphorous-based, and organic-chlorine-based pesticide-based pesticides and pesticides for animals have been used so far, but the chemical-based pesticides cause severe irritation to chickens and increase the amount It is known to cause a decrease and a decrease in spawning rate. In addition, fipronil and bipetrin have been recently detected in eggs of spawning systems treated with pesticides of chemical components, and the fipronil is a component that may cause abnormalities in functions of the liver, thyroid, and kidney in humans when consumed in large quantities. , Bipetrin is known to be a carcinogen, causing considerable social issues. In addition, there is a problem in that chicken ticks have a considerable resistance due to chemical spraying of highly toxic pesticides. Accordingly, interest in pesticides containing natural ingredients that do not cause the above-mentioned problems caused by chemical treatment as an active ingredient is higher than ever, and essential oils derived from plants and plants to find an appropriate control method for chicken ecology Based on this, there is a need for the development of natural-derived pesticides that can replace existing chemical pesticides because they have excellent fast-acting properties and insecticidal properties and are not toxic.

이에 본 발명자들은 천연 성분의 식물 추출물을 이용해 닭진드기 살충제에 관한 연구를 수행하던 중 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분, 침투이행제 및 부형제를 포함하는 살충제 조성물에 닭진드기 특이적인 살충효과가 있음을 확인하고 본 발명을 완성하였다. Accordingly, the present inventors conducted a study on chicken mite pesticides using plant extracts of natural ingredients, active ingredients for insecticidal and penetrating agents, including derris elliptica extract, paraffin, and citric anhydride as active ingredients. And it was confirmed that there is a specific insecticidal effect of chicken mite in the pesticide composition comprising an excipient and completed the present invention.

본 발명의 목적은 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분, 침투이행제 및 부형제를 포함하는 살충제 조성물, 상기 살충제 조성물의 제조방법 및 닭진드기 살충방법을 제공하는 것이다.An object of the present invention is a pesticide composition comprising an active ingredient for insecticide, a penetration agent and an excipient, comprising a derris elliptica extract, paraffin and citric anhydride as an active ingredient, a method for preparing the pesticide composition and chicken It is to provide a method of killing ticks.

상기 목적을 달성하기 위하여, 본 발명은 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분, 침투이행제 및 부형제를 포함하는 살충제 조성물을 제공한다. In order to achieve the above object, the present invention provides an insecticidal composition comprising an active ingredient for insecticide, a penetration agent, and an excipient, comprising as an active ingredient a derris elliptica extract, paraffin, and citric anhydride.

또한 본 발명은 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분, 침투이행제 및 부형제를 혼합하는 단계;를 포함하는 것을 특징으로 하는 살충제 조성물의 제조방법을 제공한다. In addition, the present invention comprises the steps of mixing an active ingredient for insecticide, a penetration agent, and an excipient containing Deris (Derris elliptica) extract, paraffin, and citric anhydride as an active ingredient; of the pesticide composition comprising Provide a manufacturing method.

또한 본 발명은 상기 살충제 조성물을 닭진드기에 처리하는 단계;를 포함하는 닭진드기의 살충방법을 제공한다.In addition, the present invention provides a method for killing chicken mites, including the step of treating the pesticide composition with chicken mites.

본 발명에 따른 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분, 침투이행제 및 부형제를 포함하는 살충제 조성물은 닭진드기에 대한 살충효과가 우수하다. 따라서 이를 주기적으로 사용할 경우, 닭 진드기의 분포를 감소시켜 가축의 스트레스를 감소시키고 닭 진드기에 의해 매개되는 타 병원체의 감염을 차단할 수 있다. 특히, 본 발명의 식물 추출물은 식물체에서 추출한 물질이므로 친환경적으로 해충방제를 할 수 있는 장점이 있고, 기존의 화학적으로 합성된 살충제와 다른 작용기작을 가지고 있어 저항성 문제도 발생하지 않아 유용하게 사용될 수 있다.The pesticide composition comprising the active ingredient for insecticidal, dermal transfer agents and excipients comprising derris elliptica extract, paraffin and citric anhydride as an active ingredient according to the present invention has excellent insecticidal effect against chicken mites. . Therefore, when it is used periodically, it is possible to reduce the distribution of chicken mites, thereby reducing the stress of livestock and blocking infection of other pathogens mediated by chicken mites. Particularly, since the plant extract of the present invention is a material extracted from a plant, it has an advantage of environmentally friendly pest control, and has a mechanism of action different from that of a conventional chemically synthesized insecticide, so that it does not cause resistance problems and can be usefully used. .

도 1은 살충제 조성물을 처리 후 닭진드기의 사멸율을 측정한 결과(A, B)를 나타낸 도이다.
도 2는 살충제 조성물의 훈증효력을 통한 닭진드기의 사멸율을 측정한 결과(A, B)를 나타낸 도이다.
도 3은 살충제 조성물의 접촉독성을 통한 닭진드기의 사멸율을 측정한 결과(A, B)를 나타낸 도이다.
도 4는 살충제 조성물 2를 처리한 후의 닭진드기 사멸효과를 확인한 도이다.
도 5는 살충제 조성물의 희석 농도별 닭진드기 성충에 관한 사멸효과 확인을 위한 실험방법을 간단히 나타낸 도이다.
도 6은 살충제 조성물의 희석 농도별 닭진드기 성충에 관한 사멸효과(A, B)를 확인한 도이다.
도 7은 살충제 조성물의 희석 농도별 닭진드기 유충에 관한 사멸효과 확인을 위한 실험방법을 간단히 나타낸 도이다.
도 8은 살충제 조성물의 희석 농도별 닭진드기 유충에 관한 사멸효과(A, B)를 확인한 도이다. 1 is a view showing the results (A, B) of measuring the kill rate of chicken mites after treating the pesticide composition.
2 is a view showing the results (A, B) of measuring the kill rate of chicken mites through the fumigation effect of the pesticide composition.
Figure 3 is a view showing the results (A, B) of measuring the kill rate of chicken mites through contact toxicity of the pesticide composition.
4 is a view confirming the killing effect of chicken mite after treating the pesticide composition 2.
5 is a diagram briefly showing an experimental method for confirming the killing effect on adult chicken mites by dilution concentration of the pesticide composition.
6 is a view confirming the killing effect (A, B) on adult chicken mite by dilution concentration of the pesticide composition.
7 is a diagram briefly showing an experimental method for confirming the killing effect on chicken mite larvae by dilution concentration of the pesticide composition.
8 is a view confirming the killing effect (A, B) on chicken mite larvae by dilution concentration of the pesticide composition.

본 발명은 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분, 침투이행제 및 부형제를 포함하는 살충제 조성물을 제공한다. The present invention provides an insecticidal composition comprising an active ingredient for insecticide, a penetration migrating agent and an excipient containing derris elliptica extract, paraffin and citric anhydride as active ingredients.

이하, 본 발명을 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail.

본 발명에서 사용하는 살충제 조성물은 살충용 활성성분 0.1 내지 55 중량부, 침투이행제 0.1 내지 5 중량부 및 부형제 35 내지 99.8 중량부를 포함할 수 있으나, 이에 제한되는 것은 아니다. The pesticide composition used in the present invention may include 0.1 to 55 parts by weight of the active ingredient for insecticide, 0.1 to 5 parts by weight of the penetrating agent, and 35 to 99.8 parts by weight of the excipient, but is not limited thereto.

본 발명의 일 유효성분 중 하나로 사용하는 데리스는 장미목의 쌍떡잎식물 강의 속씨식물이다. 데리스에 포함된 성분 중 하나인 로테논이라는 독성분은 해충구제약으로 이용되는데, 사람과 가축에는 해가 없는 것으로 알려져 있다. 상기 데리스 추출물은 공지의 추출 방법에 의해 추출될 수 있으며, 그 방법에 제한이 있는 것은 아니다. 데리스를 생존한 그대로 또는 필요에 따라 건조시킨 다음, 그대로 또는 분쇄하여 에탄올추출, 열수추출 및 초임계추출 등으로 데리스 추출물을 수득할 수 있으나, 바람직하게는 열수추출 및 초임계 추출을 이용하여 추출할 수 있다. Deris used as one of the active ingredients of the present invention is a genus seed plant of the dicotyledonous river of the rose family. One of the ingredients contained in Deris, Rotenone, is used as a pest control agent, and is known to be harmless to humans and livestock. The derris extract may be extracted by a known extraction method, and the method is not limited. The deris extract can be obtained as it survives or dried as necessary, and then pulverized as it is or by ethanol extraction, hot water extraction, and supercritical extraction, but preferably using hot water extraction and supercritical extraction. Can be extracted.

본 발명의 일 유효성분 중 하나인 파라핀(paraffin)은 CnH2n +2(n≥19)의 화학식으로 표현되는 알케인(알칸) 탄화수소를 두루 일컫으며, 물에 녹지 않으나 에테르나 벤젠, 에스터(에스테르)에는 녹는 물질로 알려져 있다. 또한 본 발명의 일 유효성분 중 하나인 구연산은 하이드록시기(-OH)를 가지는 다염기(多鹽基) 카복실산의 하나로, 많은 식물의 씨나 과즙 속에 유리상태의 산으로 함유되어 있는 성분으로 친환경 세제로 널리 사용되고 있으며, 본 발명은 바람직하게는 무수구연산을 이용할 수 있다.Paraffin, one of the active ingredients of the present invention, refers to alkane (alkane) hydrocarbons represented by the formula of C n H 2n +2 (n≥19), and does not dissolve in water, but is not soluble in ether, benzene, or ester. (Ester) is known as a soluble substance. In addition, citric acid, which is one of the active ingredients of the present invention, is one of polybasic carboxylic acids having a hydroxy group (-OH). As it is widely used, the present invention can preferably use citric anhydride.

본 발명에서 살출용 활성성분으로 사용되는 데리스 추출물, 파리핀 및 무수구연산은 상업적으로 판매되는 데리스 추출물, 파라핀 및 무수구연산을 사용할 수 있다. As the active ingredient for killing in the present invention, the deris extract, paraffin, and citric anhydride may use commercially available deris extract, paraffin, and citric anhydride.

본 발명자들은 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분을 포함하는 살충제 조성물이 닭진드기에 살충효과가 우수하다는 사실을 훈증독성 및 접촉독성 실험 등을 통해 직접적으로 검증하였다. The present inventors have demonstrated that the insecticidal composition comprising the active ingredient for insecticidal containing derris elliptica extract, paraffin and citric anhydride as an active ingredient has excellent insecticidal effect on chicken mites. It was verified directly through.

본 발명의 살충용 활성성분은 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 0.1 내지 2 : 1 내지 7 : 0.1 내지 5의 중량비로 포함할 수 있으나, 바람직하게는 0.5 내지 1.5 : 3 내지 6 : 0.3 내지 3의 중량비로 포함할 수 있고, 더욱 바람직하게는 1: 2 내지 5 : 0.5 내지 2의 중량비로 포함할 수 있으나, 이에 제한되는 것은 아니다. The active ingredient for pesticidal of the present invention may include a derris elliptica extract, paraffin, and citric anhydride in a weight ratio of 0.1 to 2: 1 to 7: 0.1 to 5, preferably 0.5 to 1.5: 3 to 6: It may be included in a weight ratio of 0.3 to 3, more preferably 1: 2 to 5: may be included in a weight ratio of 0.5 to 2, but is not limited thereto.

본 발명의 “살충용 활성성분”은 닭 진드기에 대한 효과적인 살충이 가능하고 특히 닭진드기 성충에 대하여 강력한 사멸효과를 나타내는 성분을 의미한다. The “active ingredient for insecticidal” of the present invention means an ingredient that can effectively kill chicken mites and exhibits a powerful killing effect against adult chicken mites.

본 발명의 살충제 조성물은 상기 살충용 활성성분에 바람직하게는 시트로넬라(Citronella), 정향(Clove), 시나몬(Cinnamon), 올레오레진(Oleoresin), 주정, 이코사펜타엔산(icosapentaenoic acid, IPA), 프로필렌글리콜(Propylene Glycol), 페녹시에탄올(Phenoxyethanol), 소포제, 석회, 포름산, 개똥쑥추출물 및 녹차카페인을 더 포함할 수 있으나 이에 제한되는 것은 아니다. 본 발명의 살충제 조성물은, 구체적으로 더욱 바람직하게는, 데리스 추출물 8.24%, 파라핀 29.93%, 무수구연산 9.72%, 침투이행제 0.1%, 계면활성제로서 Tween-20 28.17%, 시트로넬라(Citronella) 0.05%, 정향(Clove) 0.08%, 시나몬(Cinnamon) 0.17%, 올레오레진(Oleoresin) 0.02%, 주정 2.05%, 이코사펜타엔산(icosapentaenoic acid, IPA) 4.14%, 프로필렌글리콜(Propylene Glycol) 5.83%, 페녹시에탄올(Phenoxyethanol) 0.003%, 소포제로 글루코너 33% 희석액 0.01%, 석회 5.27%, 포름산 0.50%, 개똥쑥추출물 4.89% 및 녹차카페인 0.83%을 포함할 수 있다. 상기와 같은 함량인 경우, 식물체에서 추출한 물질을 포함하고 있어 친환경적으로 해충방제를 할 수 있는 장점이 있고, 기존의 화학적으로 합성된 살충제와 다른 작용기작을 가지고 있어 저항성 문제도 발생하지 않아 유용하게 사용될 수 있다.The pesticidal composition of the present invention is preferably citronella, clove, cinnamon, oleoresin, alcohol, icosapentaenoic acid, IPA), propylene glycol (Propylene Glycol), phenoxyethanol (Phenoxyethanol), anti-foaming agent, lime, formic acid, may further include, but not limited to, wormwood extract and green tea caffeine. Insecticide composition of the present invention, more preferably, Deris extract 8.24%, paraffin 29.93%, citric anhydride 9.72%, penetration agent 0.1%, Tween-20 28.17% as a surfactant, Citronella (Citronella) 0.05%, Clove 0.08%, Cinnamon 0.17%, Oleoresin 0.02%, Alcohol 2.05%, Icosapentaenoic acid (IPA) 4.14%, Propylene Glycol 5.83%, phenoxyethanol (Phenoxyethanol) 0.003%, as a defoamer, gluconer 33% diluent 0.01%, lime 5.27%, formic acid 0.50%, thrush extract 4.89% and green tea caffeine 0.83%. In the case of the above content, since it contains a material extracted from a plant, it has the advantage of being an environmentally friendly pest control agent, and has a mechanism of action different from that of a chemically synthesized insecticide, so it does not cause resistance problems, so it is useful You can.

본 발명에서 사용되는 용어 “침투이행제”는 살충용 활성성분을 처리하는 해충에 상기 활성성분의 침투, 확산 및 흡수를 증진시켜 살충제의 처리시 효력 지속력을 증가시키는 제제를 의미한다. 특히 해충은 그 표면에 액체성분이 닿을 경우 표면장력을 이용하여 물방울이 흘러내리도록 함으로써 체내에 침투하지 못하도록 하는 자연적인 방어시스템을 지니고 있으므로, 이와 같은 현상을 방지하기 위해, 본 발명의 살충제 조성물에 침투이행제를 혼합하여 사용할 수 있다. 상기 침투이행제는 바람직하게는 디-리모넨 오일(d-Limonene Oil), 펠라르곤산(Pelargonic acid), 에탄올, 계면활성제, 아세트산, NaCl, 수산화암모늄 및 물을 포함할 수 있으나, 이에 제한되지 않는다. The term "penetrating agent" used in the present invention refers to an agent that increases the effective lasting power in the treatment of pesticides by enhancing the penetration, diffusion and absorption of the active ingredient in pests that treat the active ingredient for pesticides. In particular, the pest has a natural defense system that prevents penetration into the body by allowing the water droplets to flow down by using the surface tension when the liquid component touches the surface, to prevent such a phenomenon, in the pesticide composition of the present invention It can be used by mixing a penetration agent. The penetration agent may preferably include, but is not limited to, di-Limonene Oil, Pelargonic acid, ethanol, surfactant, acetic acid, NaCl, ammonium hydroxide and water. .

특히 바람직한 예로, 본 발명의 살충제 조성물은 데리스 추출물 8.24%, 파라핀 29.93%, 무수구연산 9.72%, 침투이행제 0.1%, 계면활성제로서 Tween-20 28.17%, 시트로넬라(Citronella) 0.05%, 정향(Clove) 0.08%, 시나몬(Cinnamon) 0.17%, 올레오레진(Oleoresin) 0.02%, 주정 2.05%, 이코사펜타엔산(icosapentaenoic acid, IPA) 4.14%, 프로필렌글리콜(Propylene Glycol) 5.83%, 페녹시에탄올(Phenoxyethanol) 0.003%, 소포제로 글루코너 33% 희석액 0.01%, 석회 5.27%, 포름산 0.50%, 개똥쑥추출물 4.89% 및 녹차카페인 0.83% 및 침투이행제로 디-리모넨 오일(d-Limonene Oil), 펠라르곤산(Pelargonic acid), 에탄올, 계면활성제, 아세트산, NaCl, 수산화 암모늄 및 물을 포함할 수 있다. 상기와 같은 함량의 살충제 조성물은 식물체에서 추출한 물질을 포함하고 있어 친환경적으로 해충방제를 할 수 있는 장점이 있고, 기존의 화학적으로 합성된 살충제와 다른 작용기작을 가지고 있어 저항성 문제도 발생하지 않아 유용하게 사용될 수 있다. 또한 상기와 같은 함량의 살충제 조성물은 닭진드기 바람직하게는 닭진드기 성충을 접촉독성을 통해 살충제 조성물의 도포 24시간 경과 후 최대 95.6±5.6%의 사멸율을 나타내어 효과적으로 닭진드기를 사멸시킬 수 있다. 또한 본 발명의 살충제 조성물은 로즈마리(Rosemary), 마늘(Garlic), 타임(Thyme), 머스타드(Mustard), 레몬글라스(Lemon glass), 라벤더(Lavender), 티트리(Tea tree), 유칼립투스(Eucalyptus), 오레가노(oregano), 님(Neem), 참깨(Sesame), 아니스(Anise), 페퍼민트(Peppermint), 세이지(Sage) 및 난황(Egg yolk)으로 구성된 오일류 활성성분; 은행(Ginko) 추출물, 인동덩굴(Lonicera japonica Thunb) 추출물, 백출(Atractylodes) 추출물, 질경이(Plantago asiatica L) 추출물, 마치현(Portulaca oleracea) 추출물, 백리향(Thymus quinquecostatus) 추출물 및 사바딜라(Sabadilla) 추출물로 구성된 열수 천연 성분 추출물; 및 포름산(Formic acid), 카페인(caffeine), 목초산(Pyroligneous acid), 탄산수소나트륨(Sodium hydrogen carbonate), 과산화수소(Hydrogen peroxide), 니코틴(Nicotine)으로 구성된 화학물질; 로 구성된 군으로부터 선택된 하나 이상의 물질을 더 포함할 수 있다. As a particularly preferred example, the pesticide composition of the present invention is Deris extract 8.24%, paraffin 29.93%, citric anhydride 9.72%, penetration agent 0.1%, Tween-20 28.17% as a surfactant, 0.05% Citronella, and cloves (Clove) 0.08%, Cinnamon 0.17%, Oleoresin 0.02%, Alcohol 2.05%, Icosapentaenoic acid (IPA) 4.14%, Propylene Glycol 5.83%, Phenoxy Phenoxyethanol 0.003%, gluconer 33% diluent as antifoaming agent 0.01%, lime 5.27%, formic acid 0.50%, dogwormwood extract 4.89% and green tea caffeine 0.83% and di-limonene oil as penetration agent (d-Limonene Oil) , Pelargonic acid, ethanol, surfactant, acetic acid, NaCl, ammonium hydroxide and water. Since the pesticide composition of the above-mentioned content contains a material extracted from the plant, it has the advantage of being an environmentally friendly pest control agent and has a mechanism of action different from that of an existing chemically synthesized insecticide. Can be used. In addition, the pesticide composition of the above content can effectively kill chicken mites by exhibiting a kill rate of up to 95.6 ± 5.6% after 24 hours of application of the pesticide composition through contact toxicity of chicken mites, preferably chicken mites. In addition, the pesticide composition of the present invention, rosemary (Rosemary), garlic (Garlic), thyme (Thyme), mustard (Mustard), lemon glass (Lemon glass), lavender (Lavender), tea tree (Tea tree), eucalyptus (Eucalyptus) , Oregano, neem, sesame, anise, peppermint, sage and egg yolk. Ginko extract, Lonicera japonica Thunb extract, Atractylodes extract, Plantago asiatica L extract, Portulaca oleracea extract, Thymus quinquecostatus extract and Sabadilla extract Consisting hydrothermal natural ingredient extract; And chemicals composed of formic acid, caffeine, pyroligneous acid, sodium hydrogen carbonate, hydrogen peroxide, and nicotine; It may further include one or more substances selected from the group consisting of.

본 발명의 살충의 대상인 닭진드기는 그 크기가 1mm이하 통상 0.5mm 이하인 어둡고 습한 좁은 공간을 선호하는 야행성을 나타내는 해충이다. 닭 주변에 서식하고 흡혈을 통하여 닭 계두(Fowl Pox : FP)를 비롯한 질병을 매개할 수 있다. 본 발명의 살충제 조성물은 성충 및 유충을 포함한 상기 닭진드기 특이적인 살충효과를 나타내며, 바람직하게는 닭진드기 성충에 대하여 우수한 살충효과를 나타낼 수 있다. Chicken mite, the object of the present invention, is a pest that exhibits nocturnal preference for dark and humid, narrow spaces with a size of 1 mm or less and usually 0.5 mm or less. They live around chickens and can mediate diseases including chicken pox (FP) through vampire. The pesticide composition of the present invention exhibits an insecticidal effect specific to the chicken mite, including adults and larvae, and may preferably exhibit an excellent insecticidal effect against adult chicken mites.

본 발명의 살충제 조성물은 사용을 위하여 적절히 희석할 수 있으며 1:10 내지 1:200으로 희석하여 처리할 수 있으며, 바람직하게는 1:20 내지 1:100으로 희석하여 처리할 수 있으며, 가장 바람직하게는 1:50으로 희석하여 사용할 수 있다. 상기 조성물을 희석하기 위한 희석 용매로는 물, 계면활성제로 이루어진 군에서 선택된 1종 이상을 사용할 수 있으며, 예컨대 물을 사용할 수 있다.The pesticide composition of the present invention can be appropriately diluted for use and can be treated by diluting 1:10 to 1: 200, preferably diluting 1:20 to 1: 100, and most preferably Can be used diluted 1:50. As a diluting solvent for diluting the composition, one or more selected from the group consisting of water and surfactant may be used, for example, water may be used.

본 발명에서 사용하는 “부형제”는 약제를 일정한 형태를 만들기 위하여 첨가하는 물질로서 이에 한정된 것은 아니지만 계면활성제, 안정화제, 보존제 및 용매로 구성된 군으로부터 선택되는 하나 이상의 혼합물을 사용할 수 있다.The "excipient" used in the present invention is a substance that is added to form a medicament to form a certain form, but is not limited thereto, and may use one or more mixtures selected from the group consisting of surfactants, stabilizers, preservatives, and solvents.

본 발명에서 사용하는 “계면활성제”는 살충성분을 처리표면에 확산시키는 역할을 하는 환경에 친화적인 물질을 의미하며, 바람직하게는 트윈-20, 트윈-80, 프로필렌글리콜, 펙틴, 알기산나트륨, 폴리솔베이트80, 폴리인산나트륨, 액상레시틴 및 제3인산칼륨으로 구성된 군으로부터 선택된 하나 이상의 혼합물을 사용할 수 있으며, 이에 제한되지 않으나, 대량 사용에 의하여도 환경을 오염시키지 않는 것을 사용할 수 있다."Surfactant" used in the present invention means an environmentally friendly material that serves to diffuse the pesticidal component on the treated surface, preferably twin-20, twin-80, propylene glycol, pectin, sodium alginate, One or more mixtures selected from the group consisting of polysorbate 80, sodium polyphosphate, liquid lecithin, and potassium triphosphate may be used, but are not limited thereto, and those that do not pollute the environment by mass use may be used.

본 발명에서 사용하는 “안정화제”는 유기산과 활성성분이 용매에 용해 또는 분산되어 있는 상태를 안정화시켜 줌으로서 장기 보관성을 높이는데 기여하는 물질을 의미하며, 바람직하게는 잔탄검, 카보폴, 이소프로필알콜, 에탄올 및 FD330A 소포제로 구성된 군으로부터 선택된 하나 이상의 혼합물을 사용할 수 있으나, 이에 제한되지 않는다.The term “stabilizer” used in the present invention means a substance that contributes to increase long-term storage by stabilizing a state in which an organic acid and an active ingredient are dissolved or dispersed in a solvent, preferably xanthan gum, carbopol, One or more mixtures selected from the group consisting of isopropyl alcohol, ethanol and FD330A antifoaming agent may be used, but is not limited thereto.

본 발명에서 사용하는 “보존제”는 상기 유기산과 활성성분을 포함하여 용매들의 보존성을 높여주는 역할을 수행하며, 바람직하게는 페녹시에탄올, 메칠파라벤, 프로필파라벤, 벤질알콜, 감귤류추출물, 차아황산나트륨, 피로인산나트륨, 소르빈산칼륨, 식물성글리세린, 프로피온산칼슘, 프로피온산나트륨, 메타인산염, 메타인산나트륨, 포리믹스-c, 폴리믹스, 산성아호아산나트륨 및 자몽종자추출물로 구성된 군으로부터 선택된 하나 이상의 혼합물을 사용할 수 있으나, 이에 제한되지 않는다. The "preservative" used in the present invention serves to increase the preservation of solvents, including the organic acid and the active ingredient, preferably phenoxyethanol, methylparaben, propylparaben, benzyl alcohol, citrus extract, sodium hyposulfate, One or more mixtures selected from the group consisting of sodium pyrophosphate, potassium sorbate, vegetable glycerin, calcium propionate, sodium propionate, metaphosphate, sodium metaphosphate, polymix-c, polymix, sodium acid phosphite and grapefruit seed extract may be used. However, it is not limited thereto.

본 발명에서 사용하는 산화방지제는 탄산나트륨, 함수구연산, 차아황산나트륨, 아스크로빈산나트륨, 비타민E, 산성아황산나트륨 및 비타민C로 구성된 군으로부터 선택된 하나 이상의 혼합물을 사용할 수 있으나, 이에 제한되지 않는다. Antioxidants used in the present invention may use one or more mixtures selected from the group consisting of sodium carbonate, hydrous citric acid, sodium hyposulfite, sodium ascorbate, vitamin E, sodium acid sulfite and vitamin C, but is not limited thereto.

본 발명에서 사용하는 용매는 물, 에탄올, 메탄올, 에틸아세네이트, 아세톤, N-메칠피롤리돈, 디메칠설폭사이드 및 디메칠포름아마이드로 구성된 군으로부터 선택된 하나 이상의 혼합물을 사용할 수 있으나, 이에 제한되지 않는다. The solvent used in the present invention can use one or more mixtures selected from the group consisting of water, ethanol, methanol, ethyl acetate, acetone, N-methylpyrrolidone, dimethyl sulfoxide and di methyl formamide, but is not limited thereto. Does not work.

본 발명의 살충제 조성물은 유제, 유탁제, 미탁제, 약제, 분산성액제, 수화제, 입상수화제, 수용제, 캡술현탁제, 분제, 미분제, 과립제, 입제, 펠렛제, 캡슐제, 훈연제, 훈증제, 에어로졸제, 연막제, 스프레이, 페이스트, 농축액 및 젤로 구성된 군으로부터 선택된 어느 하나 이상의 형태로 제형화될 수 있으나, 이에 제한되지 않는다. The pesticide composition of the present invention is an emulsion, an emulsion, an emulsion, an agent, a dispersant, a hydrating agent, a granulating agent, a water-receiving agent, a capsul suspension, a powder, a fine powder, a granule, a granule, a pellet, a capsule, a smoker, a fumigant, It may be formulated in any one or more forms selected from the group consisting of aerosols, smokers, sprays, pastes, concentrates and gels, but is not limited thereto.

또한 본 발명은 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분, 침투이행제 및 부형제를 혼합하는 단계;를 포함하는 것을 특징으로 하는 살충제 조성물의 제조방법을 제공한다. In addition, the present invention comprises the steps of mixing an active ingredient for insecticide, a penetration agent, and an excipient containing Deris (Derris elliptica) extract, paraffin, and citric anhydride as an active ingredient; of the pesticide composition comprising Provide a manufacturing method.

본 발명의 살충제 조성물은데리스 뿌리 20kg을 분쇄한 후, 200L의 80% 에탄올에 첨가하여 실온에서 8시간동안 교반을 통하여 추출한 데리스 추출물, 미래켐에서 구입한 파라핀(paraffin) 및 무수구연산을 혼합하여 살충용 활성성분을 이용하여 살충제 조성물을 제조할 수 있다. Insecticide composition of the present invention, after crushing 20 kg of Deris root, added to 200 L of 80% ethanol, mixed with Deris extract extracted through stirring for 8 hours at room temperature, paraffin purchased from Mirae Chem, and anhydrous citric acid Insecticide compositions can be prepared using the active ingredients for pesticides.

또한 본 발명은 상기 살충제 조성물을 닭진드기에 처리하는 단계;를 포함하는 닭진드기의 살충방법을 제공한다.In addition, the present invention provides a method for killing chicken mites, including the step of treating the pesticide composition with chicken mites.

본 발명의 살충방법은 상기 살충제 조성물의 제형, 살충대상인 닭진드기 개체 수, 장소 및 방법 등의 다양한 요인을 감안하여 본 발명의 살충제 조성물을 적용시키는 것을 포함하며, 바람직하게 상기 살충제 조성물을 유효량으로 해충 또는 서식지에 처리하는 단계를 포함한다.The pesticidal method of the present invention includes applying the pesticide composition of the present invention in consideration of various factors such as the formulation of the pesticide composition, the number of chicken mites, the place and the method of pesticide, and preferably the pesticide composition in an effective amount Or treating the habitat.

본 발명의 살충방법은 상기 살충제 조성물을 사용 시에 적당한 농도로 희석하여 사용하는 방법일 수 있다. 상기 희석 용매로는 물, 계면활성제로 이루어진 군에서 선택된 1종 이상을 사용할 수 있으며, 예컨대 물을 사용할 수 있다.The pesticidal method of the present invention may be a method of diluting the pesticide composition to an appropriate concentration in use. As the diluting solvent, one or more selected from the group consisting of water and surfactant may be used, for example, water may be used.

본 발명의 살충방법은 상기 살충제 조성물을 계사 내 닭진드기 주요 서식지인 에어덕트 안쪽, 계란벨트, 케이지 윗부분 및 시료조 밑 연결부위에에 직접 살포 또는 도포하는 방법으로 살충할 수 있다.The pesticidal method of the present invention can be pesticidal by applying or spraying the pesticide composition directly on the inside of the air mite, the egg belt, the upper part of the cage and the connection part under the sample tank, which is the main habitat of chicken mites in the cage.

이하 본 발명의 이해를 돕기 위하여 바람직한 실시예를 제시한다. 그러나 하기 실시예는 본 발명을 보다 쉽게 이해하기 위하여 제공되는 것일 뿐, 이에 의해 본 발명의 내용이 한정되는 것은 아니다.Hereinafter, preferred embodiments are provided to help understanding of the present invention. However, the following examples are only provided to more easily understand the present invention, and the contents of the present invention are not limited thereby.

실시예1Example 1 . 살충제 조성물의 제조. Preparation of pesticide compositions

데리스 뿌리 20kg을 분쇄한 후, 200L의 80% 에탄올에 첨가하여 실온에서 8시간동안 교반을 통하여 추출하였다. 추출액을 여과지(advantec filter paper number2, Japan)를 이용하여 여과하였다. 여과된 추출액을 멸균된 용기에 부은 후, 57℃에서 48시간 동안 건조를 실시하였다. 이후 분말 건조된 추출물을 최종 5 mg/ml가 되도록 증류수로 용해하고, 필터(corning syringe filter, 0.2μm, Japan)를 이용하여 여과한 후 사용하였다. 상기 방법으로 추출한 데리스 추출물, 미래켐에서 구입한 파라핀(paraffin oil), 무수구연산 외 활성성분을 혼합하여 살충용 활성성분을 제조하였다. After crushing 20 kg of Deris root, it was added to 200 L of 80% ethanol and extracted through stirring at room temperature for 8 hours. The extract was filtered using filter paper (advantec filter paper number2, Japan). The filtered extract was poured into a sterilized container, and dried at 57 ° C. for 48 hours. Thereafter, the powder-dried extract was dissolved in distilled water to a final 5 mg / ml, filtered and used using a filter (corning syringe filter, 0.2 μm, Japan). Deris extract extracted by the above method, paraffin oil purchased from Mirae Chem, and an active ingredient other than citric anhydride were mixed to prepare an active ingredient for pesticide.

이외 살충용 활성성분으로 다양한 식물 추출물을 비율을 달리하여 조합하였으며, 제조된 살충제 조성물은 하기 표 1 내지 표 6과 같다.In addition, various plant extracts were combined at different ratios as active ingredients for pesticides, and the prepared pesticide compositions are shown in Tables 1 to 6.

살충제 조성물1Pesticide composition1 원료명Raw material name 성분%(v/v)Ingredient% (v / v) ParaffinParaffin 2.000%2.000% RosemaryRosemary 0.500%0.500% GarlicGarlic 0.500%0.500% ThymeThyme 0.500%0.500% MustardMustard 0.500%0.500% Lemon GrassLemon Grass 0.500%0.500% Tea treeTea tree 0.500%0.500% ClovesCloves 0.250%0.250% LavenderLavender 0.250%0.250% CinnamonCinnamon 0.250%0.250% egg oilegg oil 0.100%0.100% OreganoOregano 0.050%0.050% OleoresinOleoresin 0.050%0.050% SesameSesame 0.050%0.050% EucalyptusEucalyptus 0.050%0.050% AniseAnise 0.030%0.030% PepermintPepermint 0.015%0.015% SageSage 0.010%0.010% 주정spirits 18.263%18.263% 계면활성제(Tween-20)Surfactant (Tween-20) 30.525%30.525% IPAIPA 6.105%6.105% Propylene GlycolPropylene Glycol 6.105%6.105% GlycerinGlycerin 0.610%0.610% PhenoxyethanolPhenoxyethanol 0.010%0.010% 함수구연산Function operation 0.005%0.005% 소포제(글루코너33%희석액)Antifoaming agent (33% diluent for gluconer) 0.001%0.001% Derris추출물Derris Extract 10.000%10.000% 무수구연산-구연산삼나트륨Citric anhydride-trisodium citrate 10.000%10.000% 석회(보르도액)Lime (Bordeaux) 8.526%8.526% 과산화수소Hydrogen peroxide 3.000%3.000% 개똥쑥추출물Dogwormwood Extract 0.500%0.500% 녹차카페인Green Tea Caffeine 0.245%0.245% 計計 100.000%100.000%

살충제 조성물 2Pesticide composition 2 원료명Raw material name 성분%(v/v)Ingredient% (v / v) ParaffinParaffin 29.93%29.93% CitronellaCitronella 0.05%0.05% CloveClove 0.08%0.08% CinnamonCinnamon 0.17%0.17% OleoresinOleoresin 0.02%0.02% 주정spirits 2.05%2.05% 계면활성제(Tween-20)Surfactant (Tween-20) 28.17%28.17% IPAIPA 4.14%4.14% Propylene GlycolPropylene Glycol 5.83%5.83% PhenoxyethanolPhenoxyethanol 0.003%0.003% 소포제(글루코너33%희석액)Antifoaming agent (33% diluent for gluconer) 0.01%0.01% Derris추출물Derris Extract 8.24%8.24% 무수구연산Anhydrous citric acid 9.72%9.72% 석회(보르도액)Lime (Bordeaux) 5.27%5.27% 포름산Formic acid 0.50%0.50% 개똥쑥추출물Dogwormwood Extract 4.89%4.89% 녹차카페인Green Tea Caffeine 0.83%0.83% 침투이행제Penetration agent 0.1%0.1% 計計 100.00%100.00%

살충제 조성물 3Pesticide composition 3 원료명Raw material name 성분%(v/v)Ingredient% (v / v) CloveClove 1.00%1.00% CinnamonCinnamon 1.00%1.00% 주정spirits 0.99%0.99% 계면활성제(Tween-20)Surfactant (Tween-20) 10.00%10.00% IPAIPA 5.00%5.00% Propylene GlycolPropylene Glycol 9.00%9.00% PhenoxyethanolPhenoxyethanol 0.003%0.003% 소포제(글루코너33%희석액)Antifoaming agent (33% diluent for gluconer) 0.01%0.01% Derris추출물Derris Extract 13.00%13.00% 무수구연산Anhydrous citric acid 7.00%7.00% 빙초산Glacial acetic acid 21.00%21.00% NaClNaCl 20.00%20.00% 개똥쑥추출물Dogwormwood Extract 2.00%2.00% 정제수Purified water 10.00%10.00% 計計 100.00%100.00%

살충제 조성물 4Pesticide composition 4 원료명Raw material name 성분%(v/v)Ingredient% (v / v) FOMULA-1FOMULA-1 37.00%37.00% FOMULA-2FOMULA-2 31.50%31.50% FOMULA-3FOMULA-3 31.50%31.50% 計計 100.00%100.00%

살충제 조성물 5Pesticide composition 5 원료명Raw material name 성분%(v/v)Ingredient% (v / v) NeemNeem 1.000%1.000% CinnamonCinnamon 0.060%0.060% GarlicGarlic 0.065%0.065% 주정spirits 3.000%3.000% 계면활성제(Tween-20)Surfactant (Tween-20) 3.500%3.500% IPAIPA 3.000%3.000% Propylene GlycolPropylene Glycol 2.890%2.890% PhenoxyethanolPhenoxyethanol 0.020%0.020% 소포제(글루코너33%희석액)Antifoaming agent (33% diluent for gluconer) 0.004%0.004% Formic acidFormic acid 15.000%15.000% 무수구연산Anhydrous citric acid 4.010%4.010% p-wp-w 1.280%1.280% GlycerinGlycerin 1.100%1.100% H2O2 H 2 O 2 0.100%0.100% Acetic acidAcetic acid 1.000%1.000% Sabadilla 추출물Sabadilla extract 16.500%16.500% 백리향 추출물Thyme extract 30.000%30.000% 은행잎 추출물Ginkgo biloba extract 2.216%2.216% 개똥쑥 추출물Dogwormwood Extract 0.500%0.500% Derris 추출물Derris extract 14.750%14.750% 計計 100.00%100.00%

살충제 조성물 6Pesticide composition 6 원료명Raw material name 성분%(v/v)Ingredient% (v / v) NeemNeem 1.057%1.057% ParafinParafin 1.950%1.950% GlycerinGlycerin 0.264%0.264% 주정spirits 6.014%6.014% 계면활성제(Tween-20)Surfactant (Tween-20) 9.923%9.923% IPAIPA 3.007%3.007% Propylene GlycolPropylene Glycol 25.559%25.559% PhenoxyethanolPhenoxyethanol 0.020%0.020% 소포제(글루코너33%희석액)Antifoaming agent (33% diluent for gluconer) 0.040%0.040% 침투이행제Penetration agent 0.200%0.200% 트릴에탄올아민(TEA)Triethanolamine (TEA) 1.500%1.500% Sabadilla 추출물Sabadilla extract 13.847%13.847% 무수구연산Anhydrous citric acid 2.000%2.000% 은행잎 추출물Ginkgo biloba extract 1.200%1.200% 개똥쑥 추출물Dogwormwood Extract 2.200%2.200% Derris 추출물Derris extract 31.220%31.220% 計計 100.00%100.00%

또한, 상기 살충제 조성물 2 및 6에서 사용하는 침투이행제의 성분은 하기 표 7에 나타내었다.In addition, the components of the penetration agent used in the pesticide compositions 2 and 6 are shown in Table 7 below.

침투이행제Penetration agent 성분명Ingredient name 함유량%(v/v)Content% (v / v) d-Limonene Oild-Limonene Oil 2.00%2.00% Pelargonic acidPelargonic acid 7.00%7.00% EthanolEthanol 10.00%10.00% Sulfactant(Tween-20)Sulfactant (Tween-20) 13.00%13.00% Acetic acidAcetic acid 10.00%10.00% NaClNaCl 10.00%10.00% Ammonium Hydroxide(28%)Ammonium Hydroxide (28%) 15.00%15.00% Pure-waterPure-water 35.00%35.00% 計計 100.00%100.00%

실시예Example 2. 실험실 내 훈증독성을 통한 닭 진드기  2. Chicken mite through fumigation toxicity in the laboratory 살충력Insecticidal 실험  Experiment

상기 실시예 1에서 제조된 살충제 조성물은 천연 추출물에 휘발성 물질이 많이 함유하고 있으므로 닭진드기에 대한 훈증독성을 나타낼 수 있는지 여부를 확인하기 위하여, 실험실 내에서 closed 시스템으로 살충률을 확인하는 실험을 수행하였다.Since the pesticide composition prepared in Example 1 contains a lot of volatile substances in the natural extract, an experiment is performed to check the insecticidal rate with a closed system in the laboratory to confirm whether it can exhibit fumigation toxicity against chicken mites. Did.

상기 실시예 1에서 제조된 살충제 조성물 1을 당분야의 통상의 방법에 따라 각각 1:50, 1:100, 1:200, 1:400으로 희석한 살충제를 사용하였으며, 상기 살충제 조성물을 닭진드기에 노출시킨 후 사멸율(mortality, %)을 측정하였다. 각 실험구 당 닭진드기(D. gallinae) 100마리를 접종하였으며, 실험은 3회 반복하여 수행하였다. 살충제 조성물의 시간대별 닭진드기 사멸율(mean±sd, %)을 측정한 결과를 도 1A 및 도 1B에 나타내었다. Insecticide composition 1 prepared in Example 1 was used as a pesticide diluted 1:50, 1: 100, 1: 200, 1: 400, respectively, according to a conventional method in the art, and the pesticide composition was placed in a chicken mite. Mortality (%) was measured after exposure. 100 chicken mites (D. gallinae) were inoculated per each experimental group, and the experiment was repeated 3 times. 1A and 1B show the results of measuring the chicken mite killing rate (mean ± sd,%) by time of the pesticide composition.

상기 실험 결과를 확인한 후, 훈증효력을 확인하기 위한 추가 실험을 수행하였으며, 페트리디쉬가 아닌 뚜껑이 없는 디쉬에 살충제 조성물을 처리하여 흄후드에 배치한 후에 닭진드기 사멸율을 측정하였고, 상기 실험의 결과를 도 2A 및 B에 나타내었다. After confirming the results of the experiment, additional experiments were conducted to confirm the fumigation efficacy, and the pesticidal composition was disposed on a fume hood by treating the pesticide composition in a dish without a lid, not a petri dish, and the chicken mite killing rate was measured. The results are shown in FIGS. 2A and B.

상기 도 1A 및 B에 나타난 바와 같이, 시간대별 사멸율(%)을 측정한 결과 살충제 조성물의 희석농도 1:200 및 1:400에서는 사멸율이 12시간 이후에 최종적으로 40%, 희석농도가 1:100인 경우에는 6시간 이후에 80%의 사멸율을 나타내는 것을 확인하였다. 희석농도가 1:50인 경우에는 살충제 조성물 도포 후 3시간 이후부터 99.4%의 높은 사멸율을 나타내는 것을 확인하여 100%에 가까운 강력한 닭진드기에 대한 사멸효과가 나타내는 것을 확인하였다.As shown in Figures 1A and B, as a result of measuring the killing rate (%) by time, the dilution concentration 1: 200 and 1: 400 of the pesticide composition, the killing rate is finally 40% after 12 hours, the dilution concentration is 1 In the case of: 100, it was confirmed that the mortality rate was 80% after 6 hours. When the dilution concentration was 1:50, it was confirmed that a high killing rate of 99.4% was observed after 3 hours after application of the pesticide composition, indicating that the killing effect on a powerful chicken mite close to 100% was exhibited.

또한 도 2A 및 2B에 나타난 바와 같이, 뚜껑이 없는 디쉬를 사용한 실험에서는 희석농도가 1:100 이상인 경우에는 훈증독성을 거의 나타내지 않았으나, 1:50으로 희석한 경우에는 살충제 조성물의 도포 후 12시간 이후부터 약 30%이상의 닭 진드기 사멸율을 나타내는 것을 확인하였다. 상기 결과를 통하여 천연 추출물을 포함하는 실시예 1의 살충제 조성물이 훈증 효력이 존재함을 확인하였다.In addition, as shown in FIGS. 2A and 2B, in the experiment using a dish without a lid, when the dilution concentration was 1: 100 or more, it showed little fumigation toxicity, but when diluted to 1:50, 12 hours after application of the pesticide composition From the results, it was confirmed that the chicken mite killing rate was about 30% or more. Through the above results, it was confirmed that the pesticide composition of Example 1 containing the natural extract had a fumigation effect.

실시예Example 3.  3. 닭진드기에Chicken mite 대한 실험실 내 접촉독성  Contact toxicity in Korean laboratory 살충력Insecticidal 실험  Experiment

상기 실시예 1에서 제조된 6가지 살충제 조성물이 접촉독성에 의한 닭진드기 살충률 효과를 갖는지 확인하기 위하여 실험실 내에서 open 시스템으로 실험을 수행하였다.In order to confirm whether the six pesticide compositions prepared in Example 1 have an effect of killing chicken mites due to contact toxicity, experiments were conducted with an open system in a laboratory.

시료로 상기 실시예 1에서 제조된 살충제 조성물 1 내지 6을 각각 1:25, 1:50, 1:100, 1:200으로 희석한 살충제를 사용하였으며, 상기 살충제를 닭진드기에 노출시킨 후 사멸율(mortality, %)을 측정하였다. 각 실험구 당 닭진드기(D. gallinae) 100마리를 접종하였으며, 실험은 3회 반복하여 수행하였다. 각 살충제의 접촉독성을 확인하기 위하여 살충제 제제의 처리 후 24시간 뒤의 농도별 닭진드기 사멸율(mean±sd, %)을 측정한 결과를 도 3A 및 B에 나타내었고, 살충제 조성물 2를 사용해 닭진드기 살충한 결과를 도 4에 나타내었다.As a sample, the pesticide compositions 1 to 6 prepared in Example 1 were diluted with 1:25, 1:50, 1: 100, and 1: 200, respectively, and the mortality rate after exposing the pesticide to chicken mites (mortality,%) was measured. 100 chicken mites (D. gallinae) were inoculated per each experimental group, and the experiment was repeated 3 times. In order to confirm the contact toxicity of each pesticide, the results of measuring the chicken mite killing rate (mean ± sd,%) by concentration after 24 hours after treatment of the pesticide formulation are shown in FIGS. 3A and B, and the chicken using the pesticide composition 2 The results of the insecticidal mite are shown in FIG. 4.

도 3A 및 B에 나타난 바와 같이, 살충제 조성물 2, 3, 5, 6을 사용하는 경우, 희석된 비율이 가장 낮은 1:25 비율에서 80% 이상의 닭진드기 사멸율이 나타나는 것을 확인하였으며, 상기 시료들의 희석비율이 높아질수록 살충 효과가 낮아지는 전반적인 살충 양상을 확인하였다. 특히 살충제 조성물로 가장 활용도가 높게 사용할 수 있는 1:50 비율로 희석한 실험군에서 살충제 조성물 2 및 6을 도포한 경우, 24시간 후에 최대 95.6±5.6%와 86.5±5.8%라는 높은 값을 나타내어 상기 살충제 조성물의 접촉 독성을 통한 닭진드기 살충 효과가 현저하게 우수함을 확인하였다. 3A and B, when using the pesticide compositions 2, 3, 5, and 6, it was confirmed that the chicken mite killing rate of 80% or more was observed at a 1:25 ratio with the lowest diluted ratio. The higher the dilution ratio, the lower the pesticidal effect was confirmed. In particular, when the pesticide compositions 2 and 6 are applied in an experimental group diluted at a ratio of 1:50 that can be used most highly as a pesticide composition, after 24 hours, the maximum values of 95.6 ± 5.6% and 86.5 ± 5.8% are shown, and the insecticide It was confirmed that the insecticidal effect of chicken mite through contact toxicity of the composition was remarkably excellent.

또한 도 4에서 확인한 바와 같이, 살충제 조성물 2를 사용한 경우 조성물의 처리 24시간 내에 닭진드기가 접촉독성에 의하여 효과적으로 살충됨을 확인하였다.In addition, as shown in Figure 4, when using the pesticide composition 2, it was confirmed that the chicken mite is effectively killed by contact toxicity within 24 hours of treatment of the composition.

실시예4Example 4 . . 닭진드기Chicken mite 성충 및 유충에 관한  About adult and larva 살충력의Insecticidal 검증 Verification

4.1 4.1 닭진드기Chicken mite 성충에 관한 살충 효과의 확인 Confirmation of insecticidal effects on adult insects

닭진드기 살충 효과를 확인한 살충제 조성물 1, 2, 3, 5가 닭진드기 성충에 관하여 살충효과를 나타내는지 여부를 확인하기 위하여 in vitro, semi-field 환경에서 실험을 수행하였다.In order to confirm whether the insecticidal composition 1, 2, 3, or 5, which confirmed the insecticidal effect of chicken mite, shows an insecticidal effect with respect to adult chicken mite, experiments were conducted in an in vitro and semi-field environment.

살충제 조성물 1, 2, 3, 5을 각각 1:50, 1:100, 1:200인 3개 구간으로 희석하여 닭진드기 성충 100마리에 접종시킨 후 24시간 경과 후의 사멸율(mortality, %)을 측정하였다. 실험은 3회 반복하여 수행하였으며, 상기 실험 수행 방법을 간단히 도 5에 나타내었고, 살충제 조성물의 처리 후 24시간 내의 농도별 닭진드기 성충 사멸율(mean±sd, %)을 in vitro, semi-field에서 측정한 결과를 도 6A 및 B에 나타내었다.The insecticidal compositions 1, 2, 3, and 5 were diluted in three sections of 1:50, 1: 100, and 1: 200, respectively, and inoculated into 100 adult chicken mites, resulting in mortality (%) after 24 hours. It was measured. The experiment was repeated 3 times, and the method for performing the experiment was briefly shown in FIG. 5, and the killing rate (mean ± sd,%) of adult chicken mites by concentration within 24 hours after treatment of the pesticide composition was measured in vitro and semi-field. The results measured in are shown in Figures 6A and B.

도 6A 및 B에 나타난 바와 같이, 살충제 조성물 2는 1: 200의 희석농도의 경우에도 semi-field 및 in vitro에서 각각 57.8±2.7% 또는 68.5±4.5%로 대조군 대비 절반 이상의 살충력을 나타내었고, 1:100의 경우 64.5±4.5% 또는 77.5±1.5%의 살충력을 나타내는 것을 확인하였다. 또한 1:50의 희석농도에서는 각각의 실험에서 94.7±7.7% 또는 95.5±1.3%를 나타내는 것을 확인하여 성충에 관해서 100%에 가까운 닭진드기 성충에 대한 방제 효과를 나타내는 것으로 확인하였다.As shown in Figures 6A and B, pesticide composition 2 exhibited more than half the insecticidal power compared to the control group at 57.8 ± 2.7% or 68.5 ± 4.5% in semi-field and in vitro, respectively, even at a dilution concentration of 1: 200, 1 : In the case of 100, 64.5 ± 4.5% or 77.5 ± 1.5% was found to exhibit insecticidal properties. In addition, at a dilution concentration of 1:50, it was confirmed that each experiment showed 94.7 ± 7.7% or 95.5 ± 1.3%, indicating that it showed a control effect against adult chicken mite adults as close to 100% as to adult.

4.2 4.2 닭진드기Chicken mite 유충에 관한 살충 효과의 확인 Confirmation of insecticidal effects on larvae

닭진드기 살충 효과를 확인한 살충제 조성물 1, 2, 3, 5가 닭진드기 유충에 관한 살충효과를 나타내는지 여부를 확인하기 위하여 in vitro 환경에서 실험을 수행하였다.Experiments were conducted in an in vitro environment to determine whether pesticide compositions 1, 2, 3, and 5, which confirmed the effect of chicken mite insecticidal, show insecticidal effects on chicken mite larvae.

살충제 조성물 1, 2, 3, 5를 각각 1:50, 1:100, 1:200인 3개 구간으로 희석하여 살충제로 닭진드기 유충 20마리에 접종시킨 후 24시간 경과 후의 사멸율(mortality, %)을 측정하였다. 실험은 3회 반복하여 수행하였으며, 상기 실험 수행 방법을 간단히 도 7에 나타내었고, 살충제 조성물의 처리 후 24시간 뒤의 농도별 닭진드기 유충 사멸율(mean±sd, %)을 in vitro에서 측정한 결과를 도 8A 및 B에 나타내었다.The insecticidal compositions 1, 2, 3, and 5 were diluted in three sections of 1:50, 1: 100, and 1: 200, respectively, and inoculated into 20 chicken mite larvae with pesticides. ) Was measured. The experiment was repeated three times, and the method for performing the experiment was briefly shown in FIG. 7, and the chicken mite larvae kill rate (mean ± sd,%) by concentration was measured in vitro 24 hours after treatment of the pesticide composition. The results are shown in FIGS. 8A and B.

도 8A 및 B에 나타난 바와 같이, 살충제 조성물 1은 유충에 관하여 가장 높은 정도의 살충력을 나타내고 1:50의 희석된 비율에서 70% 이상의 살충력을 나타내는 것으로 확인되었다. 또한 상기 실시예 4.1에서 우수한 닭진드기 성충 살충력을 나타내는 살충제 조성물 2는 유충에 대해서 1:50로 희석된 경우에도 43.3±30.6%정도의 유충 살충력을 나타내는 것을 확인하였다. As shown in Figures 8A and B, the pesticide composition 1 was found to exhibit the highest degree of insecticidal activity with respect to the larvae and an insecticidal activity of 70% or more at a diluted ratio of 1:50. In addition, it was confirmed that the insecticidal composition 2 showing excellent chicken mite adult insecticidal activity in Example 4.1 exhibits a larval insecticidal activity of 43.3 ± 30.6% even when diluted 1:50 with respect to the larvae.

상기와 같은 결과를 통해, 본 발명의 살충제 조성물이 닭진드기 성충 및 유충에 관하여 방제효과가 우수한 것을 확인하였고, 실제 양계 현장에서 발생하는 닭진드기로 인해 발생하는 양계업계의 경제적 이익이 기하급수적으로 감소하는 현실을 감안할 때, 상기와 같은 본 발명의 방제효과를 통해 양계업계의 이익을 획기적으로 보전할 수 있을 것으로 판단된다. Through the above results, it was confirmed that the pesticidal composition of the present invention has excellent control effect with respect to adult and adult larvae of chicken mite, and the economic benefit of the poultry industry caused by chicken mites occurring in the actual poultry field decreases exponentially. Given the reality of the above, it is determined that it is possible to significantly preserve the interests of the poultry industry through the control effect of the present invention as described above.

Claims (20)

데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분;
시트로넬라(Citronella), 정향(Clove), 시나몬(Cinnamon), 올레오레진(Oleoresin), 주정, 이코사펜타엔산(icosapentaenoic acid, IPA), 프로필렌글리콜(Propylene Glycol), 페녹시에탄올(Phenoxyethanol), 소포제, 석회, 포름산, 개똥쑥추출물, 녹차카페인 및 침투이행제를 포함하고,
상기 침투이행제는 디-리모넨 오일(d-Limonene Oil), 펠라르곤산(Pelargonic acid), 에탄올, 계면활성제, 아세트산, NaCl, 수산화 암모늄 및 물을 포함하는 것을 특징으로 하는, 닭진드기 살충제 조성물.
Deris (Derris elliptica) extract, paraffin (paraffin) and an active ingredient for insecticide containing citric anhydride as an active ingredient;
Citronella, Clove, Cinnamon, Oleoresin, Alcohol, Icosapentaenoic Acid (IPA), Propylene Glycol, Phenooxyethanol ), Anti-foaming agent, lime, formic acid, thrush wormwood extract, green tea caffeine and penetrating agent,
The penetration agent is di-limonene oil (d-Limonene Oil), pelargonic acid (Pelargonic acid), ethanol, surfactant, acetic acid, NaCl, ammonium hydroxide and water, characterized in that the chicken mite pesticide composition.
제1항에 있어서, 상기 살충제 조성물은 살충용 활성성분 0.1 내지 55 중량부, 침투이행제 0.1 내지 5 중량부 및 부형제 40 내지 99.8 중량부를 포함하는 것을 특징으로 하는, 닭진드기 살충제 조성물.According to claim 1, wherein the pesticide composition is characterized in that it comprises 0.1 to 55 parts by weight of the active ingredient for insecticide, 0.1 to 5 parts by weight of the penetration agent, and 40 to 99.8 parts by weight of the excipient, chicken mite pesticide composition. 제1항에 있어서, 상기 살충용 활성성분은 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 1 : 2 내지 5 : 0.5 내지 2의 중량비로 포함하는 것을 특징으로 하는, 닭진드기 살충제 조성물.According to claim 1, wherein the active ingredient for insecticidal, characterized in that it comprises a derris (Derris elliptica) extract, paraffin (paraffin) and citric anhydride in a weight ratio of 1: 2 to 5: 0.5 to 2, chicken mite pesticides Composition. 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분;
글리세린, 이코사펜타엔산(icosapentaenoic acid, IPA), 프로필렌글리콜(Propylene Glycol), 페녹시에탄올(Phenoxyethanol), 소포제, 주정, 님 (Neem), 개똥쑥추출물, 트릴에탄올아민 (triethanolamine, TEA), 사바딜라 (Sabadilla) 추출물, 은행잎 추출물 및 침투이행제를 포함하고,
상기 침투이행제는 디-리모넨 오일(d-Limonene Oil), 펠라르곤산(Pelargonic acid), 에탄올, 계면활성제, 아세트산, NaCl, 수산화 암모늄 및 물을 포함하는 것을 특징으로 하는, 닭진드기 살충제 조성물.Deris (Derris elliptica) extract, paraffin (paraffin) and an active ingredient for insecticide containing citric anhydride as an active ingredient;
Glycerin, icosapentaenoic acid (IPA), propylene glycol, phenoxyethanol, antifoaming agent, alcohol, neem, thrush extract, triethanolamine (TEA), Contains Sabadilla extract, Ginkgo biloba extract and Penetrating agent,
The penetration agent is di-limonene oil (d-Limonene Oil), pelargonic acid (Pelargonic acid), ethanol, surfactant, acetic acid, NaCl, ammonium hydroxide and water, characterized in that the chicken mite pesticide composition. 제4항에 있어서, 상기 살충제 조성물은 살충용 활성성분 0.1 내지 55 중량부, 침투이행제 0.1 내지 5 중량부 및 부형제 40 내지 99.8 중량부를 포함하는 것을 특징으로 하는, 닭진드기 살충제 조성물.
According to claim 4, The pesticide composition is characterized in that it comprises 0.1 to 55 parts by weight of the active ingredient for insecticide, 0.1 to 5 parts by weight of the penetration agent and 40 to 99.8 parts by weight of the excipient, chicken mite insecticide composition.
삭제delete 삭제delete 삭제delete 제1항 또는 제4항에 있어서, 상기 닭진드기는 닭진드기 성충인 것을 특징으로 하는, 닭진드기 살충제 조성물.The chicken mite pesticide composition according to claim 1 or 4, wherein the chicken mite is an adult chicken mite. 제1항에 있어서, 상기 살충제 조성물은 1:20 내지 1:100의 부피비율로 희석된 것을 특징으로 하는, 닭진드기 살충제 조성물.According to claim 1, The pesticide composition is characterized in that diluted in a volume ratio of 1:20 to 1: 100, chicken mite pesticide composition. 제1항 또는 제4항에 있어서, 계면활성제, 안정화제, 보존제, 산화방지제 및 용매로 구성된 군으로부터 선택되는 하나 이상의 부형제를 포함하는 것을 특징으로 하는 닭진드기 살충제 조성물.The chicken mite pesticide composition according to claim 1 or 4, comprising at least one excipient selected from the group consisting of surfactants, stabilizers, preservatives, antioxidants and solvents. 제11항에 있어서, 상기 계면활성제는 트윈-20, 트윈-80, 프로필렌글리콜, 펙틴, 알기산나트륨, 폴리솔베이트80, 폴리인산나트륨, 액상레시틴 및 제3인산칼륨으로 구성된 군으로부터 선택된 하나 이상의 혼합물인 것을 특징으로 하는 닭진드기 살충제 조성물.12. The method of claim 11, wherein the surfactant is at least one selected from the group consisting of tween-20, tween-80, propylene glycol, pectin, sodium alginate, polysorbate 80, sodium polyphosphate, liquid lecithin and potassium triphosphate Chicken mite pesticide composition characterized in that the mixture. 제11항에 있어서, 상기 안정화제는 잔탄검, 카보폴, 이소프로필알콜 및 에탄올로 구성된 군으로부터 선택된 하나 이상의 혼합물임을 특징으로 하는 닭진드기 살충제 조성물.12. The chicken mite pesticide composition of claim 11, wherein the stabilizer is at least one mixture selected from the group consisting of xanthan gum, carbopol, isopropyl alcohol and ethanol. 제11항에 있어서, 상기 보존제는 페녹시에탄올, 메칠파라벤, 프로필파라벤, 벤질알콜, 감귤류추출물, 차아황산나트륨, 피로인산나트륨, 소르빈산칼륨, 식물성글리세린, 프로피온산칼슘, 프로피온산나트륨, 메타인산염, 메타인산나트륨, 포리믹스-c, 폴리믹스, 산성아호아산나트륨 및 자몽종자추출물로 구성된 군으로부터 선택된 하나 이상의 혼합물임을 특징으로 하는 닭진드기 살충제 조성물.12. The method of claim 11, wherein the preservative is phenoxyethanol, methyl paraben, propyl paraben, benzyl alcohol, citrus extract, sodium hyposulfate, sodium pyrophosphate, potassium sorbate, vegetable glycerin, calcium propionate, sodium propionate, metaphosphate, sodium metaphosphate , Polymix-c, polymix, acidic sodium nitrite and grapefruit seed extract, characterized in that at least one mixture selected from the group consisting of chicken mite pesticide composition. 제11항에 있어서, 상기 산화방지제는 탄산나트륨, 함수구연산, 차아황산나트륨, 아스크로빈산나트륨, 비타민E, 산성아황산나트륨 및 비타민C로 구성된 군으로부터 선택된 하나 이상의 혼합물임을 특징으로 하는 닭진드기 살충제 조성물.12. The chicken mite pesticide composition of claim 11, wherein the antioxidant is at least one mixture selected from the group consisting of sodium carbonate, hydrous citric acid, sodium hyposulfite, sodium ascorbate, vitamin E, acidic sodium sulfite and vitamin C. 제11항에 있어서, 상기 용매는 물, 에탄올, 메탄올, 에틸아세네이트, 아세톤, N-메칠피롤리돈, 디메칠설폭사이드 및 디메칠포름아마이드로 구성된 군으로부터 선택된 하나 이상의 혼합물임을 특징으로 하는 닭진드기 살충제 조성물.The chicken according to claim 11, wherein the solvent is one or more mixtures selected from the group consisting of water, ethanol, methanol, ethyl acetate, acetone, N-methylpyrrolidone, dimethyl sulfoxide and di methyl formamide. Mite pesticide composition. 제1항 또는 제4항에 있어서, 상기 살충제 조성물은 유제, 유탁제, 미탁제, 약제, 분산성액제, 수화제, 입상수화제, 수용제, 캡술현탁제, 분제, 미분제, 과립제, 입제, 펠렛제, 캡슐제, 훈연제, 훈증제, 에어로졸제, 연막제, 스프레이, 페이스트, 농축액 및 젤로 구성된 군으로부터 선택된 어느 하나의 형태로 제형화된 것을 특징으로 하는 닭진드기 살충제 조성물.The method according to claim 1 or 4, wherein the pesticide composition is an emulsion, an emulsion, an emulsion, a drug, a dispersible liquid, a hydrating agent, a granulating hydrating agent, a water-receiving agent, a capsul suspending agent, a powder, a fine powder, a granulating agent, a granulating agent, a pelleting agent. , Capsules, smokers, fumigants, aerosols, smokers, sprays, pastes, concentrates, and gel mite pesticide composition characterized in that formulated in any one form selected from the group consisting of gels. 데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분,
시트로넬라(Citronella), 정향(Clove), 시나몬(Cinnamon), 올레오레진(Oleoresin), 주정, 이코사펜타엔산(icosapentaenoic acid, IPA), 프로필렌글리콜(Propylene Glycol), 페녹시에탄올(Phenoxyethanol), 소포제, 석회, 포름산, 개똥쑥추출물, 녹차카페인, 침투이행제 및 부형제를 혼합하는 단계;를 포함하고,
상기 침투이행제는 디-리모넨 오일(d-Limonene Oil), 펠라르곤산(Pelargonic acid), 에탄올, 계면활성제, 아세트산, NaCl, 수산화 암모늄 및 물을 포함하는 것을 특징으로 하는, 닭진드기 살충제 조성물의 제조방법.Deris (Derris elliptica) extract, paraffin (paraffin) and an active ingredient for insecticide containing citric anhydride as an active ingredient,
Citronella, Clove, Cinnamon, Oleoresin, Alcohol, Icosapentaenoic Acid (IPA), Propylene Glycol, Phenooxyethanol ), Mixing the antifoaming agent, lime, formic acid, thrush extract, green tea caffeine, penetrating agent and excipient;
The penetration agent is characterized in that it comprises a di-limonene oil (d-Limonene Oil), pelargonic acid (Pelargonic acid), ethanol, surfactant, acetic acid, NaCl, ammonium hydroxide and water, chicken mite pesticide composition Manufacturing method. 제1항 또는 제4항의 살충제 조성물을 닭진드기에 처리하는 단계;를 포함하는 닭진드기의 살충방법.
Claim 1 or 4 of the insecticidal composition comprising the step of treating a chicken mite; insecticidal method of containing chicken mites.
데리스(Derris elliptica) 추출물, 파라핀(paraffin) 및 무수구연산을 유효성분으로 포함하는 살충용 활성성분;
글리세린, 이코사펜타엔산(icosapentaenoic acid, IPA), 프로필렌글리콜(Propylene Glycol), 페녹시에탄올(Phenoxyethanol), 소포제, 주정, 님 (Neem), 개똥쑥추출물, 트릴에탄올아민 (triethanolamine, TEA), 사바딜라 (Sabadilla) 추출물, 은행잎 추출물, 침투이행제 및 부형제를 혼합하는 단계; 를 포함하고,
상기 침투이행제는 디-리모넨 오일(d-Limonene Oil), 펠라르곤산(Pelargonic acid), 에탄올, 계면활성제, 아세트산, NaCl, 수산화 암모늄 및 물을 포함하는 것을 특징으로 하는, 닭진드기 살충제 조성물의 제조방법.Deris (Derris elliptica) extract, paraffin (paraffin) and an active ingredient for insecticide containing citric anhydride as an active ingredient;
Glycerin, icosapentaenoic acid (IPA), propylene glycol, phenoxyethanol, antifoaming agent, alcohol, neem, thrush extract, triethanolamine (TEA), Mixing sadilla (Sabadilla) extract, ginkgo biloba extract, penetrating agent and excipients; Including,
The penetration agent is characterized in that it comprises a di-limonene oil (d-Limonene Oil), pelargonic acid (Pelargonic acid), ethanol, surfactant, acetic acid, NaCl, ammonium hydroxide and water, chicken mite pesticide composition Manufacturing method.
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