KR101849123B1 - Pesticide Composition for Corythucha dliata - Google Patents

Abstract

The present invention relates to an insecticidal composition for controlling insect pest insects, more particularly, to a insecticidal composition for controlling insect pest insects comprising a plant extract or an essential oil.
The insecticidal composition for controlling insecticidal wood shield insecticide according to the present invention contains at least one selected from the group consisting of patchouli, cinnamon, clove, benzoin, palmarosa and suprapuric supercritical fluid extract.
The plant extracts, essential oils and the compounds isolated from plants according to the present invention are harmless to the human body and the environment, and are excellent in insecticidal effect against sycamorea shield worms.

Description

{Pesticide Composition for Corythucha dliata}

The present invention relates to an insecticidal composition for controlling insect pest insects, more particularly, to a insecticidal composition for controlling insect pest insects comprising a plant extract or an essential oil.

 "Avenue of trees" is a tree planted in or around a road for the purpose of improving the living and traffic environment, such as the creation of beautiful scenery, reduction of environmental pollution and recording, and maintaining the connectivity of natural ecosystems. It means planted so that there is no obstacle to the securing of traffic.

Among the trees, Platanus orientalis L. is a deciduous broad-leaved arboreous tree that is strong against the pollution and excellent in air purification power, and is planted as a lot of trees and trees in various parts of Korea throughout the country.

However, in recent years, the damage caused by shield insects has been spread nationwide, and it has become necessary to pay particular attention to the control of vassal trees.

Platanus occid enta is mainly applied in the United States, but it is also known to attack ash trees and mulberry trees. It is known to occur more than twice a year.

Since June when the temperature is rising in Korea, moths and shields of various kinds of adults and nymphs simultaneously live on the backside of the host plants and the leaves are turned into yellowish white. It is greatly hurting.

At present, there are two kinds of biological control (biogeochemistry, predatory natural and hospital microorganism), chemical control (insecticide), forestry control (selection of insecticide variety, thinning) and mechanical and physical control The chemical control using the insecticide is widely used because its effect is accurate and rapid.

Korean Patent Registration No. 0750648 discloses a method for producing a compound of formula (I), which comprises using acetamiprid, magnesium, glycerin fatty acid ester and sodium-5-mononitro-guaiacol as an active ingredient and using an inert carrier or a diluent, Have been disclosed.

However, the chemical control is considered to be toxic to humans, adverse effects on forest ecosystems, particularly the effects on natural creatures including natural enemies, the recurrence of damage due to the rapid recovery of pest density after control and the emergence of resistant (resistance) pests, It is necessary to develop a natural insecticide having excellent insecticidal activity while minimizing such side effects.

Accordingly, the present inventors have made efforts to develop a natural insecticide for P. vannamei, and have found that the extract obtained by supercritical extraction of the superfine tree under specific conditions; Essential oils such as patchouli, cinnamon, cloves, benzoin, and palmarosa oil; Isolation from plants such as Geraniol, Cinnamaldehyde, Eucalyptol, Citronellal, (+) - α-Pinene and Menthol And that the resulting compound has an excellent insecticidal effect against P. vannamei. The present invention has been completed based on this finding.

An object of the present invention is to provide an insecticidal composition for controlling insect pest insects comprising plant extracts, essential oils or compounds isolated from plants, which are harmless to humans and environment and have excellent insecticidal activity against P. vannamei, And to provide a method for controlling shield worms.

In order to accomplish the above object, the present invention provides a method for producing a sicilian shield worm, which comprises at least one selected from the group consisting of patchouli, cinnamon, clove, benzoin, An insecticide composition for controlling insects is provided.

In the present invention, the supercritical fluid extract of supercritical fluid comprises: (a) (b) pulverizing the dried primavera to a particle size of 0.5 to 3 mm; And (c) supercritical extraction of the crushed pre-pine wood at a temperature of 40 to 60 DEG C and a pressure of 100 to 500 bar.

According to the present invention, the supernatant fluid extract of patchouli, cinnamon, clove, benzoin, palmarosa or suprapriole is contained in an amount of 0.1 to 50 wt% based on the total weight of the composition. .

The present invention also relates to a method for the preparation of a pharmaceutical composition comprising Geraniol, Cinnamaldehyde, Eucalyptol, Citronellal, (+) - alpha -pinene and Menthol, Wherein the insecticidal composition comprises at least one compound selected from the group consisting of: < RTI ID = 0.0 > (I) < / RTI >

The present invention also provides a method of controlling the vesicle shield worms using the insecticidal composition for controlling the vesicle shield worms.

The plant extracts, essential oils and the compounds isolated from plants according to the present invention are harmless to the human body and the environment, and are excellent in insecticidal effect against sycamorea shield worms.

FIG. 1 is a picture showing the insecticidal activity of the insecticidal composition for controlling insect shields against P. vannamei according to the present invention.
FIG. 2 is a picture showing the insecticidal activity of the insecticidal composition for controlling insect shields against P. vannamei according to the present invention.

In the present invention, a substance having insecticidal activity against P. vannamei was examined from an environmentally friendly plant.

In the present invention, the insecticidal effect of Supercritical Fluid Extract, Methanol Extract, and Essential Oil of Plants on Platycodon sp.

In one embodiment of the present invention, an extract obtained by supercritical extraction of a superfine tree under a specific condition; It was confirmed that essential oils such as patchouli, cinnamon, clove, benzoin, and palmarosa oil have an insecticidal effect against P. vannamei.

Accordingly, the present invention, in one aspect, is characterized in that it comprises at least one selected from the group consisting of patchouli, cinnamon, clove, benzoin, palmarosa and suprapuric supercritical fluid extract ≪ / RTI >

In the present invention, the supercritical fluid extract of supercritical fluid comprises: (a) (b) pulverizing the dried primavera to a particle size of 0.5 to 3 mm; And (c) supercritical extraction of the crushed pre-pine wood at a temperature of 40 to 60 DEG C and a pressure of 100 to 500 bar.

As used herein, the term " primavera " means a deciduous shrub with hornblende.

In the present invention, the branch portion of the superfine tree is used.

The step of cold drying the primavera is a pretreatment step for increasing the extraction efficiency of the primavera. That is, when drying is performed by cold air, it is possible to prevent the volatilization of the functional components in the early volatiles which can be easily volatilized at high temperatures.

The term 'cold air drying' as used in the present invention is a term in contrast to 'hot air drying', which means a drying method of drying by cold air at room temperature. The temperature during the cold air drying is preferably in the range of 15 to 40 ° C.

In the present invention, the cold air drying can be carried out for several days, preferably for 1 to 5 days, and can be appropriately adjusted according to the moisture content of the primrose.

The step of pulverizing the dried primate to a particle size of 0.5 to 3 mm is a pretreatment step for increasing extraction efficiency.

The pulverization is preferably carried out with particles having a size of 0.5 to 3 mm, more preferably pulverized into particles having a size of 1 to 2 mm. If the particle size during the milling is out of the above range, the extraction efficiency may be lowered. Particularly, when the particle size is less than 0.5 mm, the extraction efficiency is lowered due to the pressure load during the supercritical extraction, and the volatile active ingredient is volatilized during the pulverization process. On the other hand, when the particle size is larger than 3 mm, the functional components contained in the particles are difficult to be extracted to the outside by the supercritical fluid.

The step of supercritical carbon dioxide extraction is carried out at a temperature of 40 to 60 ° C. and a pressure of 100 to 500 bar, preferably a pressure of 50 ° C. and a pressure of 300 bar.

In the supercritical extraction step, if the supercritical extraction is carried out under a temperature or pressure condition out of the above range, the compound having insecticidal effect against the P. vannamei shield worms is not extracted or the yield thereof is lowered even if it is extracted .

In the present invention, the supercritical fluid is not particularly limited, but carbon dioxide is more preferable. Carbon dioxide is a solvent mainly used in supercritical fluid extraction. After extraction, it is vaporized under atmospheric pressure and released into the air. Therefore, it is non-toxic and non-hazardous due to the absence of residual solvent and can be used in the food and drug manufacturing industry have.

The flow rate of the supercritical fluid according to the present invention is preferably 50 to 70 ml / min. If the flow rate is less than 50 ml / min, the yield is good, but the efficiency with time is low. If the flow rate is more than 70 ml / min, the yield may be lowered because proper extraction is not performed.

In the present invention, the supercritical extraction is characterized by extracting supercritical carbon dioxide and alcohol as a solvent in order to increase the extraction yield of the active ingredient. That is, the extraction yield can be increased by using the supercritical fluid as a co-solvent while extracting it with supercritical fluid.

In the present invention, it is preferable that the patchouli, cinnamon, clove, benzoin, palmarosa, or suprapurus supercritical fluid extract is contained in an amount of 0.1 to 50 wt%, respectively, based on the total weight of the composition.

When the content ratio of the patchouli, cinnamon, clove, benzoin, palmarosa, or suprapurus supercritical fluid extract is less than 0.1 wt% based on the total weight, the insecticidal effect against the desired platycodon shield worm of the present invention is small When the amount of the insecticide is more than 50% by weight based on the total weight of the insecticidal composition, it is difficult to exhibit an appropriate insecticidal effect due to a relatively low addition amount of a general composition. In addition, Problems can arise.

In the meantime, the present invention has been made to confirm whether or not the insecticidal effect of a plant-derived single component of P. vannamei is observed.

 In another embodiment of the present invention there is provided a pharmaceutical composition comprising Geraniol, Cinnamaldehyde, Eucalyptol, Citronellal, (+) - alpha -pinene, menthol Menthol) were found to have insecticidal effects against the moth, Shield worm.

Thus, the present invention provides, in a further aspect, a pharmaceutical composition comprising Geraniol, cinnamaldehyde, Eucalyptol, (±) -citronellal, (+) - (Menthol). ≪ Desc / Clms Page number 2 >

In the present invention, the above-mentioned Geraniol, cinnamaldehyde, Eucalyptol, (±) -citronellal, (+) - α-pinene or menthol ) Is preferably contained in an amount of 0.1 to 20% by weight relative to the total weight of the composition.

The inclusion ratio of Geraniol, cinnamaldehyde, Eucalyptol, (±) -citronellal, (+) - α-pinene or menthol is higher than that of Geraniol, Cinnamaldehyde, Eucalyptol, If the amount is less than 0.1% by weight based on the total weight, the insecticidal effect against the desired P. vannamei may be small, and if it exceeds 20% by weight based on the total weight, It may be difficult to exhibit an appropriate insecticidal effect due to the blending ratio, and there may arise a problem that economic considerations must be taken into account when preparing insecticides.

In addition, the present invention provides a method for controlling P. vannamei using an insecticidal composition for controlling P. vannamei.

The insecticidal composition for controlling insect pest of the present invention according to the present invention may be applied to a conventional insecticide treatment, immersion treatment or fumigation treatment. In this case, it is possible to apply a conventional insecticide.

The insecticide composition may be prepared and applied in various forms and may be prepared in the form of a solution, emulsion, coating or granule by dissolving or dispersing in at least one solvent for application and immersion treatment, and optionally adding a surfactant Is preferably used in the form of an additive, for example, a liquid solvent, a pressurized liquefied gas and / or a solid carrier in the form of a fuming agent, a fumigant, an application agent, a granule and a solid agent.

In order to prepare these various types of preparations, supercritical fluid extracts, essential oils, and plant-derived compounds according to the present invention may be used as solvents and additives commonly used in the art. For example, ethanol or water, which is harmless to the human body, can be prepared into a liquid form using a dispersion, and can be prepared into a solid form using conventional excipients and the like. Also, the concentration of the active ingredient in the pesticide is used in an effective amount to effect the insecticidal action on the vesicle shield worm, and the specific concentration thereof depends on the form of the preparation and the application method.

[Example]

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Example 1: Preparation of plant supercritical fluid extract

(GCT-0615, Green Kultek) for 1 day at 30 ° C and then pulverized to a particle size of about 2 mm using a pulverizer.

Using the supercritical extraction system (ISA-SCFE-0050-0100-080, Ilshin Autoclave, Korea) according to the extraction conditions of Table 1 and the extraction method of Table 2, the extracts of Ganghwam, Gosam, Chappy, Supercritical extraction was performed, and the extraction yield was shown in Table 3.

Extraction tank assistant
menstruum Auxiliary solvent flow rate
(ml / min) Separator extraction
time
(min) CO ₂
flux
(ml / min) Pressure (bar) Temperature (℃) pressure
(bar) Temperature
(° C) 200 50 spirits 3 ml / min 60 min injection 40 40 120 60 300 50 spirits 3 ml / min 60 min injection 40 40 120 60 400 50 spirits 3 ml / min 60 min injection 40 40 120 60

Time (minutes) 10 20 30 40 50 60 70 80 90 100 110 120 Experimental course SCF CO ₂ injection only SCF CO ₂ + alcohol injection SCF CO ₂ injection only

Name of sample Extraction tank Co-solvent
(spirits)
Flow rate per minute Injection
Amount of sample
(g) Extraction amount after concentration (g) extraction
yield
(%) pressure
(bar) Temperature
(° C) Ganghwa 200 50 3ml 50 1.3 2.6 300 200 7.5 3.8 400 45 2.8 6.2 GoSam 200 50 3ml 50 0.8 1.6 300 200 2.3 1.2 400 43 0.3 0.7 Chuppy 200 50 3ml 50 0.5 One 300 150 1.7 1.1 400 50 0.5 One Celestial 200 50 3ml 50 1.3 2.6 300 200 6.3 3.2 400 50 1.0 2 Textiles 200 50 3ml 100 4.6 4.6 300 100 6.1 6.1 400 100 6.7 6.7 Mold 200 50 3ml 50 1.2 2.4 300 150 2.6 1.7 400 50 1.4 2.8

In Table 3, 1.3 g (yield: 2.6%) of gypsum, 0.8 g (yield: 1.6%) of gosam, 0.5 g of superfine wood (yield: 1.0%) and 1.3 g (Yield: 3.8%) and supernatant fluid extraction (50 ℃, 300 bar) were obtained, respectively. In the supercritical fluid extraction (50 ℃, 300 bar) (Yield: 1.2%), superfine tree (1.7 g) (yield: 1.1%), 6.3 g of celestial gong (yield: 3.2% (Yield: 0.7%), supernatant (0.5g) and 1.0g (yield: 2%) of supercritical fluid extraction (50 ℃ and 400 bar) ), 6.7 g (yield: 6.7%), and 1.4 g of mold extract (yield: 2.8%).

Example 2 Production of Solvent Extract of Plant

300 g of dried ganghwil, gangam, hapi (phytoplankton), celestial gypsum, whitish white and mold were added to 3.6 L of methanol and extracted for 62 hours. After filtration, the mixture was concentrated under reduced pressure (rotary vacuum evaporator; EYELA) to give 45.3 g (yield: 15.1% Methanol extracts of 18.8 g (yield: 6.3%) of ginseng, 6.18 g (yield: 2.1%), 34.99 g (yield: 11.7%) and 11.2 g (yield: 3.7%) were obtained.

Example 3: Evaluation of the insecticidal effect of Supercritical Fluid Extracts, Solvent Extracts and Essential Oils of Plants on Platycodon sp.

Supercritical fluid extracts of Ganghwam, Gamgam, P. japonicus, Astragalus membranaceus, Eucalyptus and Thunb. Prepared in Example 1, methanol extracts of the plants prepared in Example 2 and 65 essential oils were prepared according to Table 4 , And they were exposed to indoor moths 24 hours after the treatment.

Active ingredient 2% One% 0.5 Plant extracts and essential oils 0.4 mL 0.2 mL 0.1 mL Tween-80 0.6 mL 0.4 mL 0.2 mL Ethyl-alcohol 1.0 mL 0.4 mL 0.2 mL water 18.0 mL 19.0 mL 19.5 mL

That is, the spray formulation prepared in the concentration shown in Table 4 was evenly contacted with a piece of filter paper of 9 cm in diameter, and then dried in a fume hood for 20 seconds, and filter paper in which ethanol was volatilized was placed in a petri dish (φ 9 cm). Next, 25 petal platypus shield worms attached to the host plant were counted in the petri dish, and the lid was covered and evaluated for the insecticidal activity after 24 hours. The results were shown in Table 5 (treated with 2%) and Table 6 (1). The results of the insecticidal efficacy were shown in Table 5 % Treatment) and Table 7 (0.5% treatment). All tests were repeated three times.

Plant Type Mortality (%, mean ± SE) Plant Type Mortality (%, mean ± SE) Sweet orange 28.0 ± 4.0 Galanum 88.0 ± 4.0 Tangerine Aroma 66.7 ± 6.1 Nana Mint 93.3 ± 6.1 Eucalyptus Lemon 48.0 ± 4.0 Neolith 97.3 ± 4.6 Kajjekt 76.0 ± 4.0 camphor tree 69.3 ± 2.3 Eucalyptus radiata 77.3 ± 4.6 Laban Dean 94.7 ± 4.6 Citronella 100 ± 0 Lemon balm 98.7 ± 2.3 Lemon grass 98.7 ± 2.3 Manuka 97.3 ± 4.6 May Window 88.0 ± 4.0 Mandarin (green) 86.7 ± 4.6 Greyfruit 48.0 ± 4.0 Mandarin (Red) 72.0 ± 4.0 Lime 28.0 ± 4.0 Myrrh 90.7 ± 2.3 Cedarwood 66.7 ± 4.6 mint 92.0 ± 4.0 Niauli 89.3 ± 2.3 Bay 89.3 ± 2.3 lemon 70.7 ± 2.3 Benzoin 100 ± 0 Rosemary 38.7 ± 2.3 Black pepper 78.7 ± 2.3 Spearmint 61.3 ± 2.3 St. John's Wort 41.3 ± 2.3 Palmarosa 100 ± 0 Pine tree 77.3 ± 4.6 Pine Aroma 88.0 ± 4.0 Spruce 89.3 ± 4.6 Peppermint 100 ± 0 Andes Bernina 69.3 ± 2.3 cypress 77.3 ± 2.3 Angelica 77.3 ± 4.6 Clarice is. 68.0 ± 4.0 Orange 80.0 ± 4.0 Petgrain 89.3 ± 2.3 grapefruit 89.3 ± 4.6 Juniper Berry 49.3 ± 2.3 Jasmine 96.0 ± 4.0 Frankinsense 36.0 ± 4.0 fir 57.3 ± 2.3 Bergamot Calabria 77.3 ± 4.6 cloves 100 ± 0 Carrot seed 78.7 ± 2.3 Cham parka 77.3 ± 4.6 Patchouli 100 ± 0 Chamomile 98.7 ± 2.3 basil 88.0 ± 4.0 Car foot 81.3 ± 2.3 Lavender Bulgaria 90.7 ± 2.3 cacao 97.3 ± 4.6 Marjoram 77.3 ± 4.6 Calderman 96.0 ± 4.0 Ylang ylang 78.7 ± 2.3 Camper 58.7 ± 2.3 Mir 40.0 ± 4.0 Common 97.3 ± 2.3 Cinnamon 100 ± 0 Howood 100 ± 0 Thinning (supercritical extraction) 88.0 ± 4.0 Fleece (methanol extract) 83.3 ± 2.6 Ganghwam (supercritical extraction) 97.3 ± 4.6 Ganghwam (methanol extraction) 89.3 ± 2.3 Gosam (Supercritical Extraction) 94.7 ± 4.6 Gosam (methanol extract) 92.0 ± 4.0 Mold (supercritical extraction) 97.3 ± 4.6 Mold (methanol extract) 93.3 ± 6.1 Superfine (supercritical extraction) 100 ± 0 Second peach tree (methanol extract) 97.3 ± 2.3 Cantilever (supercritical extraction) 78.7 ± 2.3 Tongkung (methanol extract) 77.3 ± 6.1

Plant Type Mortality
(%, mean + SE) Plant Type Mortality
(%, mean + SE) Kajjekt 62.7 ± 6.1 Galanum 81.3 ± 4.6 Eucalyptus radiata 69.3 ± 2.3 Nana Mint 85.3 ± 9.2 Citronella 100 ± 0 Neolith 90.7 ± 2.3 Lemon grass 96.0 ± 4.0 camphor tree 58.7 8.3 May Window 81.3 ± 6.1 Laban Dean 85.3 ± 6.1 Cedarwood 57.3 ± 6.1 Lemon balm 88.0 8.0 Niauli 80.0 ± 4.0 Manuka 94.7 ± 4.6 lemon 58.7 ± 4.6 Mandarin (green) 80.0 + - 6.9 Palmarosa 98.7 ± 2.3 Mandarin (Red) 62.7 ± 8.3 Pine Aroma 78.7 ± 2.3 Myrrh 80.0 + - 6.9 Peppermint 94.7 ± 4.6 mint 82.7 ± 2.3 cypress 68.0 ± 6.9 Bay 81.3 ± 2.3 Clarice is. 53.3 ± 2.3 Benzoin 100 ± 0 Petgrain 78.7 ± 2.3 Black pepper 65.3 ± 4.6 Bergamot Calabria 68.0 ± 4.0 Pine tree 61.3 ± 6.1 Carrot seed 62.7 ± 4.6 Spruce 80.0 ± 4.0 Patchouli 100 ± 0 Andes Bernina 58.7 ± 2.3 basil 80.0 ± 4.0 Angelica 68.0 ± 4.0 Lavender Bulgaria 81.3 ± 2.3 Orange 69.3 ± 9.2 Marjoram 68.0 ± 4.0 grapefruit 82.7 ± 6.1 Ylang ylang 62.7 ± 2.3 Jasmine 82.7 ± 8.3 Cinnamon 100 ± 0 cloves 100 ± 0 Thinning (supercritical extraction) 80.0 + - 6.9 Cham parka 66.7 ± 6.1 Ganghwam (supercritical extraction) 85.3 ± 6.1 Chamomile 85.3 ± 6.1 Gosam (Supercritical Extraction) 86.7 8.3 Car foot 68.0 ± 4.0 Mold (supercritical extraction) 90.7 ± 6.1 cacao 93.3 8.3 Superfine (supercritical extraction) 97.3 ± 4.6 Calderman 85.3 ± 6.1 Cantilever (supercritical extraction) 69.3 ± 2.3 Common 86.7 ± 4.6 Ganghwam (methanol extraction) 81.3 ± 2.3 Howood 92.0 ± 4.0 Gosam (methanol extract) 81.3 ± 4.6 Second peach tree (methanol extract) 88.0 ± 6.9 Mold (methanol extract) 84.0 ± 6.9 Tongkung (methanol extract) 62.7 ± 8.3

Plant Type Mortality (%, mean ± SE) Plant Type Mortality (%, mean ± SE) Citronella 77.3 8.3 mint 57.3 ± 2.3 Lemon grass 77.3 ± 6.1 Bay 61.3 ± 6.1 May Window 64.0 ± 10.6 Benzoin 88.0 ± 4.0 Niauli 62.7 ± 4.6 Spruce 60.0 + - 4.0 Palmarosa 85.3 ± 6.1 grapefruit 62.7 ± 2.3 Pine Aroma 68.0 ± 4.0 Jasmine 58.7 ± 6.1 Peppermint 80.0 ± 4.0 cloves 89.3 ± 2.3 Petgrain 61.3 ± 6.1 Chamomile 70.7 ± 15.1 Patchouli 92.0 ± 4.0 cacao 80.0 + - 6.9 basil 65.3 ± 6.1 Calderman 69.3 ± 6.1 Lavender Bulgaria 62.7 ± 2.3 Common 69.3 ± 9.2 Cinnamon 97.3 ± 4.6 Howood 70.7 8.3 Galanum 66.7 ± 9.2 Thinning (supercritical extraction) 64.0 8.0 Nana Mint 73.3 ± 10.1 Ganghwam (supercritical extraction) 68.0 + - 12.0 Neolith 78.7 ± 2.3 Gosam (Supercritical Extraction) 60.0 8.0 Laban Dean 70.7 8.3 Mold (supercritical extraction) 72.0 ± 4.0 Lemon balm 68.0 ± 10.6 Superfine (supercritical extraction) 84.0 ± 6.9 Manuka 82.7 ± 8.3 Ganghwam (methanol extraction) 65.3 ± 9.2 Mandarin (green) 65.3 ± 12.9 Gosam (methanol extract) 60.0 + - 4.0 Myrrh 54.7 8.3 Second peach tree (methanol extract) 64.0 ± 13.9

As shown in Tables 5 and 7, 62% of the plants showed insecticidal activity of 50% or more at the concentration of 2%, insecticidal activity of 50% or more at 40% at the concentration of 0.5%, and more than 80% (92.0%), cinnamon 97.3%, clove 89.3%, benzoin 88.0%, palmarosa 85.3% and supercritical fluid extract (84.0 ± 6.9%) showed high insecticidal activity I could.

Example 4: Assessment of the insecticidal effect of plant-derived single component of P. vannamei

The results are shown in Table 8, and the results are shown in Table 8. The results are shown in Table 8 below. Respectively.

Compounds Mortality (%, mean ± SE) Compounds Mortality (%, mean ± SE) (-) - Limonene oxide 12.1 ± 2.1 Isobornyl acetate 0 ± 0.0 (-) - Terpinen-4-ol 67.5 ± 3.5 Linalool 55.8 ± 2.4 (-) - Verbenone 24.4 ± 1.5 Linalyl acetate 31.6 ± 4.9 (-) -? - Pinene 46.8 ± 5.4 Menthol 80.2 ± 5.3 (+) - Limonene oxide 68.7 ± 5.1 Menthone 76.3 ± 3.2 (+) - Pulegone 11.4 ± 7.1 Sabinene hydrate 0 ± 0.0 (+) -? - Pinene 87.4 ± 3.8 Sabinene 78.5 ± 3.4 (+) -? - Pinene 78.6 ± 2.2 Terpinen-4-ol 66.5 ± 2.1 (±) -Citronellal 88.7 ± 4.5 Terpineol 55.6 ± 3.3 (1 R) - (+) - a-Pinene 55.4 ± 5.2 Thymol 28.1 ± 4.5 (R) - (+) - Limonene 75.6 ± 3.8 Valencene 0 ± 0.0 (S) - (-) - Limonene 81.3 ± 2.3 α, β-Thujone 70.2 ± 3.2 1-Undecyne 0 ± 0.0 α-Pinene oxide 0 ± 0.0 3-Carene 5.4 ± 1.2 α-Pinene 66.9 ± 1.2 Camphor 5.3 ± 2.2 α-Terpinene 55.4 ± 1.6 Cinnamaldehyde 89.2 ± 3.1 α-Terpineol 35.3 ± 3.4 Citral 0 ± 0.0 β-Caryophyllene 25.1 ± 2.3 Eucalyptol 87.5 ± 3.5 β-Thujaplicin 35.8 ± 3.2 Geraniol 91.5 ± 4.3

From Table 8, Geraniol showed the highest insecticidal activity (91.5%), and Cinnamaldehyde 89.2%, Eucalyptol 87.5%, (±) -Citronellal 88.7%, (+) - α-Pinene 87.4% and Menthol 80.2% It was confirmed that it exhibited a high insecticidal activity.

Example 5: Evaluation of the effect of outdoor insecticidal effect on the moth,

5-1: Production of formulations

The stability of the formulation, the sprayability and the preservability of the formulation were evaluated in order to confirm whether the composition for insecticidal insect control of P. vannamei was usable as a formulation, and the results are shown in Table 9.

The stability of the formulation was determined by keeping the combination composition of Cinnamon, Patchouli and Palmarosa oil, which had the highest activity, at room temperature, and then, after one week, there was a change in properties such as separation and precipitation.

The sprayability of the formulation was evaluated by spraying a plaster spray composition containing a cinnamon, patchouli and palmarosa oil into a plastic spray container.

Container storability was determined by injecting a composition for insecticidal insecticidal insecticidal control of P. vannamei into a spray container, and then checking the appearance of the container after one week.

Formulation (Composition) Stability of formulation Ability to dispense formulations Container
Preservability Cinnamon Essential Oil 5% + Tween 80 15% + Ethanol 80% Great Great normal 5% + Tween 80 15% + Ethanol 80% Great Great normal Palmarosa oil 5% + Tween 80 15% + Ethanol 80% Great Great normal Cinnamon Essential Oil 4% + Exp. Rio 4% + Palmarosa Oil 4% + Tween 80 8% + Ethanol 80% Great Great normal

As shown in Table 9, it was confirmed that the formulation containing Cinnamon, Patchouli and Palmarosa oil was excellent in stability and jetting ability, and that the preservability of the container was normal.

5-2: Assessment of outdoor insecticidal effect on Shrimp Shield worms of formulation

500 ml of the formulation prepared in Example 5-1 was prepared and sprayed twice onto 25 individuals of Platycodon sp. At this time, the injection amount was 90 to 100 μl. The insecticidal activity was evaluated 24 hours after the spraying treatment, and the results are shown in Table 10. The results of the insecticidal activity were observed in the host plants after 24 hours treatment, or the insect pincushion insect which was dropped on the insect pine no. 6 was regarded as the larvae with no movement, and all tests were repeated three times .

Formulation (Composition) Mortality (%, mean ± SE) Cinnamon Essential Oil 5% + Tween 80 15% + Ethanol 80% 100 ± 0 5% + Tween 80 15% + Ethanol 80% 92.1 ± 3.7 Palmarosa oil 5% + Tween 80 15% + Ethanol 80% 89.5 ± 2.4 Cinnamon Essential Oil 4% + Exp. Rio 4% + Palmarosa Oil 4% + Tween 80 8% + Ethanol 80% 100 ± 0 Fenthion 0.25% (control) 94.2 ± 3.8

From Table 10, it can be seen that the cinnamon essential oil 5% + Tween 80 15% + 80% ethanol 80% formulation, 5% fetidolil + 15% + Tween 80 15% + ethanol 80% formulation 92.1%, palmarosa oil 5% + Tween 80 15 % + Ethanol 80% formulation showed 89.5% insecticidal activity, cinnamon essential oil 4% + 4% + 4% palmarosa oil + 8% Tween 80 + ethanol 80% .

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereto will be. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

Wherein the insecticidal composition for controlling insect shields of Platycodon grandiflorum is characterized in that it contains an extract of P. cinerea.
2. The method according to claim 1,
(a) a step of cold-drying the primavera;
(b) pulverizing the dried primavera to a particle size of 0.5 to 3 mm; And
(c) Supercritical extraction of the crushed pre-pine wood at a temperature of 40 to 60 DEG C and a pressure of 100 to 500 bar.
The insecticidal composition according to claim 1, wherein the extract comprises 0.1 to 50 wt% of the total weight of the composition.
delete Use of a composition according to any one of claims 1 to 3 for the control of pollinated wood worms.

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