KR102601133B1 - Eco-friendly smokescreen composition for control of Dermanyssus gallinae comprisingplant extracts, producing method thereof, and control method of Dermanyssus gallinae using it - Google Patents
Eco-friendly smokescreen composition for control of Dermanyssus gallinae comprisingplant extracts, producing method thereof, and control method of Dermanyssus gallinae using it Download PDFInfo
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- KR102601133B1 KR102601133B1 KR1020210049668A KR20210049668A KR102601133B1 KR 102601133 B1 KR102601133 B1 KR 102601133B1 KR 1020210049668 A KR1020210049668 A KR 1020210049668A KR 20210049668 A KR20210049668 A KR 20210049668A KR 102601133 B1 KR102601133 B1 KR 102601133B1
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Classifications
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- A—HUMAN NECESSITIES
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
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- A—HUMAN NECESSITIES
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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- Botany (AREA)
- Toxicology (AREA)
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Abstract
본 발명은 파라핀오일, 카란자오일, 계피오일, 정향오일 및 양명아주 추출물을 유효성분으로 포함하고, 님오일과 Tween-80(Polyoxyethylene Sorbitan Monooleate)을 연막용 확산제로 포함하는, 닭진드기 방제용 연막조성물, 이의 제조방법 및 이를 이용한 닭진드기의 방제방법에 관한 것이다.
본 발명의 닭진드기 방제용 연막조성물은 천연 식물추출물을 사용함으로써 인축 및 환경에 영향을 주지 않으면서 닭진드기를 효과적으로 방제할 수 있고, 기존의 살충제 농약과 작용기작이 다르므로 약제저항성 문제도 발생하지 않으며, 잔류농약의 문제도 발생하지 않으며, 연막처리를 함으로써 분무 등 종래 처리방법에 비하여 닭진드기에 대한 방제활성이 매우 우수하다.The present invention is a smoke screen for controlling chicken mites, which contains paraffin oil, caranja oil, cinnamon oil, clove oil, and extract of Yangmyeong Aster as active ingredients, and neem oil and Tween-80 (Polyoxyethylene Sorbitan Monooleate) as a smoke spreader. It relates to a composition, a method for producing the same, and a method for controlling chicken mites using the same.
The smoke screen composition for controlling chicken mites of the present invention can effectively control chicken mites without affecting livestock and the environment by using natural plant extracts, and has a different mechanism of action from existing pesticides and pesticides, so it does not cause drug resistance problems. There is no problem with residual pesticides, and the control activity against chicken mites is very excellent compared to conventional treatment methods such as spraying by using fog treatment.
Description
본 발명은 식물추출물을 포함하는 친환경 닭진드기 방제용 연막조성물, 이의 제조방법 및 이를 이용한 닭진드기 방제방법에 관한 것으로, 보다 구체적으로는 파라핀오일, 카란자오일, 계피오일, 정향오일 및 양명아주 추출물을 유효성분으로 포함하고 님오일과 Tween-80을 연막용 확산제로 포함하는 닭진드기 방제용 연막조성물과, 이를 제조하는 방법 및 이를 이용하여 닭진드기를 방제하는 방법에 관한 것이다.The present invention relates to an eco-friendly smoke screen composition for controlling chicken mites containing plant extracts, a method for manufacturing the same, and a method for controlling chicken mites using the same, and more specifically, paraffin oil, Carranza oil, cinnamon oil, clove oil, and Yangmyeong root extract. It relates to a smoke screen composition for controlling chicken mites containing as an active ingredient and neem oil and Tween-80 as a smoke spreader, a method of manufacturing the same, and a method of controlling chicken mites using the same.
닭진드기(Dermanyssus gallinae, Red-mite, 일명 와구모)는 1mm 크기의 아열대성 외부기생충으로 계사내 틈새나 분변에 있다가 어두워지면 닭에 붙어 피를 먹는다. 닭진드기는 곤충이 아닌 거미과 생물이다.Chicken mites (Dermanyssus gallinae, Red-mite, also known as Wagumo) are subtropical external parasites measuring 1 mm in size. They live in crevices or feces inside the chicken house and attach to chickens when it gets dark and feed on their blood. Chicken mites are not insects but spiders.
산란계 양계장의 경우 닭진드기의 침투율은 23%에서 최대 90%까지로 매우 높은 상황이다. 침투한 닭진드기는 닭의 산란율을 10~15% 감소시키고, 계란의 중량을 2.2% 감소시키며 사료효율을 5.7%까지 감소시킬 뿐만 아니라 닭에게 가려움증과 스트레스를 유발하는 동시에 살모넬라 등 질병의 매개체 역할을 한다. 최근 닭진드기를 방제하기 위하여 처리한 농약이 계란에 축적되어 잔류하여 사회적 물의를 야기한 바 있다.In the case of laying hen farms, the penetration rate of chicken mites is very high, ranging from 23% to a maximum of 90%. Infiltrated chicken mites not only reduce chicken egg laying rate by 10-15%, egg weight by 2.2%, and feed efficiency by 5.7%, but also cause itchiness and stress in chickens, while acting as vectors for diseases such as salmonella. do. Recently, pesticides treated to control chicken mites accumulated and remained in eggs, causing social controversy.
닭진드기는 약충에서 흡혈을 시작해 성충까지 일주일 이상 흡혈을 하며 흡혈시에만 축체에 기생하면서 보통 30~60분간 흡혈하고 흡혈후에는 산란, 소화, 번식을 위해 계사에 숨어 버린다. 닭진드기가 산란하기에 적정한 온도는 25~30℃이고 성장을 위한 적정온도는 25~37℃이다. Chicken ticks start sucking blood as nymphs and continue to drink blood for more than a week until they become adults. They parasitize on the brood body only when sucking blood, usually for 30 to 60 minutes, and after sucking blood, they hide in the chicken house to lay eggs, digest, and reproduce. The optimal temperature for chicken mites to lay eggs is 25-30℃ and the optimal temperature for growth is 25-37℃.
닭진드기는 전세계적으로 문제가 되고 있으나 닭진드기의 예방이나 구제방법에 대한 정보는 부족한 실정이다. Chicken mites are a problem worldwide, but there is a lack of information on how to prevent or eliminate chicken mites.
현재 국내에서 닭진드기의 방제를 위하여 사용되는 약제는 비펜쓰린, 싸이퍼메트린 등 피레스로이드(pyrethroid)계, 비피엠씨 등 카바메이트(carbamate)계, 유기인(organophosphorus)계 농약 및 포름산(formic acid)이 대표적이다. 또한 허가를 받지 않은 페닐피라조에(phenylpyrazoe)계의 피프로닐(fipronil)이 사용되어 최근 문제가 된 바가 있다. Currently, the drugs used in Korea to control chicken mites include pyrethroids such as bifenthrin and cypermethrin, carbamates such as BPM, organophosphorus pesticides, and formic acid. This is representative. In addition, there has been a recent problem due to the use of fipronil, a phenylpyrazoe product that has not been approved.
이처럼 방제 효과를 빠르게 얻기 위해 화학 살충제를 사용하고 있지만, 화학 살충제는 비용이 많이 들고 토양을 오염시키며 생태계의 균형을 파괴할 우려가 있을 뿐만 아니라, 지속적으로 살포하는 경우 이들 살충제 농약에 대한 저항성을 나타내는 닭진드기의 출현이 빠르게 일어나서 방제를 더욱 어렵게 하고 있다.In order to quickly achieve this control effect, chemical pesticides are used, but chemical pesticides are not only expensive, pollute the soil, and have the risk of destroying the balance of the ecosystem, but also show resistance to these pesticides if sprayed continuously. Chicken mites appear rapidly, making control more difficult.
지금까지 닭진드기에 대한 물리적, 생물학적 방제방법에 대한 다양한 연구가 진행중이지만 천연물을 이용한 친환경 약제에 의한 닭진드기의 방제방법으로 실용화된 것은 은행잎, 님오일, 정향추출물을 이용한 것만이 알려져 있어 개발히 극히 미진한 실정이다. So far, various studies are in progress on physical and biological control methods for chicken mites, but the only known practical methods for controlling chicken mites using eco-friendly chemicals using natural products are ginkgo leaves, neem oil, and clove extract, so development is extremely limited. The situation is insufficient.
또한 종래의 친환경 약제를 이용한 닭진드기 방제방법은 연막처리가 아니라 분무 등의 방법으로 약제를 계사에 처리하고 있다.In addition, the conventional method of controlling chicken mites using eco-friendly chemicals is to treat the chemicals to the chicken house using methods such as spraying rather than fogging.
카란자(Karanja, Pongamia glabra)는 인도, 미얀마, 스리랑카, 말라카해협의 섬 등에서 자생하는 관목으로, 비누, 보습을 위한 헤어 및 바디오일, 살충제 등으로 사용되고 있다. 카란자오일(Karanja oil)은 카란자의 씨앗에서 추출한 것으로, 카란자오일의 주성분은 카라진(Karanjin)이다. 카란자오일은 카란자 씨를 냉압착법으로 추출하며, 연한 호박색을 띠는 유동성 액체로 무독성, 무자극성이다. 카란자오일은 살충 및 방부제로 널리 알려져 있으며, 살충효과가 있어서 진드기, 벼룩, 옴 등을 포함하는 다양한 해충을 방제하는 효과가 있고, 보습효과를 가지므로 습진, 건선, 피부 궤양, 비듬 치료 등에도 사용할 수 있다. 카란자오일은 님오일(neem oil)과 비슷한 효능을 지니고 있지만 향에서 차이가 있다.Karanja (Pongamia glabra) is a shrub that grows wild in India, Myanmar, Sri Lanka, and islands in the Strait of Malacca, and is used as soap, moisturizing hair and body oil, and pesticides. Karanja oil is extracted from Karanja seeds, and its main ingredient is Karanjin. Carranza oil is extracted from Carranza seeds using a cold-pressing method, and is a light amber-colored fluid liquid that is non-toxic and non-irritating. Carranza oil is widely known as an insecticide and preservative. It has an insecticidal effect and is effective in controlling various pests, including ticks, fleas, and scabies. It also has a moisturizing effect, so it is also used in the treatment of eczema, psoriasis, skin ulcers, and dandruff. You can use it. Carranza oil has similar effects to neem oil, but has a different scent.
정향나무(Clove, Eugenia caryophyllata)는 도금양과에 속하는 상록교목으로 인도네시아가 원산지이다. 말린 꽃봉오리가 마치 못과 닮았다고 해서 정향이라는 이름으로 불리게 되었다. 정향오일(clove-oil)는 정향나무의 꽃눈 또는 잎으로부터 얻은 오일성분으로, 정향오일의 수율은 봉오리(clove bud oil)는 17~20%, 꽃자루(clove stem oil)는 약 6%, 잎(clove leaf oil)은 2~3%이다. 정향오일의 주성분은 유제놀(Eugenol)이며 그밖에 이소-유제놀(iso-eugenol) 및 카리오필렌(caryophyllene) 성분을 포함하고 있다. 정향은 향기가 좋을 뿐 아니라, 향료 중에서 부패방지효과, 살균력 및 살충력이 매우 우수하다. Clove (Eugenia caryophyllata) is an evergreen tree belonging to the Myrtle family and is native to Indonesia. It was called clove because the dried flower buds resembled nails. Clove oil is an oil component obtained from the flower buds or leaves of the clove tree. The yield of clove oil is 17-20% for clove bud oil, about 6% for clove stem oil, and about 6% for clove oil (clove oil). clove leaf oil) is 2-3%. The main component of clove oil is eugenol, and it also contains iso-eugenol and caryophyllene. Clove not only has a good scent, but also has excellent anti-corruption, sterilizing, and insecticidal effects among fragrances.
계피(桂皮, Cinnamon)는 계피나무의 줄기 및 가지의 껍질을 벗기고, 코르코층을 다소 제거하여 말린 것으로 빛깔은 황갈색이다. 계피는 스리랑카가 전세계 시장의 70%를 점유하고 있으며, 남인도, 수마트라, 보르네오 등에서 많이 생산되고 있다. 계피는 독특한 향기가 있고 맛은 떫으나 달며 점액성이다. 계피는 여러가지 성분을 함유하고 있는데 계피정유(Essential oil)에는 1~4%의 계피유가 함유되어 있고, 계피유의 주성분은 시남알데하이드(Cinnamaldehyde)이다. 계피의 향기성분은 알레르기를 일으키는 집먼지 진드기 등을 죽이는 데 효과가 있다고 알려져 있다.Cinnamon is made by peeling the bark of the trunk and branches of the cinnamon tree, removing some of the cork layer, and drying it. It is yellow-brown in color. Sri Lanka accounts for 70% of the global market for cinnamon, and a lot of it is produced in South India, Sumatra, and Borneo. Cinnamon has a unique scent and tastes astringent but sweet and mucilaginous. Cinnamon contains various components. Essential oil contains 1-4% cinnamon oil, and the main component of cinnamon oil is cinnamaldehyde. Cinnamon's fragrant ingredients are known to be effective in killing house dust mites that cause allergies.
양명아주(Chenopodium ambrosioides)는 명아주과의 일년초이다. 양명아주는 남아메리카가 원산지인 귀화식물로 우리나라에서는 덕천강을 중심으로 전 구간에 귀화되어 있다. 줄기는 가지를 많이 치고, 위쪽에 성긴 털이 있으며, 높이는 30~80 cm 수준으로 꽃은 6~10월 사이에 핀다. 잎은 장 타원상 피침형으로 어긋나고 가장자리에 크기가 다른 톱니가 있으며, 엽병은 짧고 뒷면에는 담황색 선점이 있다. 맥 위에 다세포 털이 있으며 위쪽 잎은 톱니가 없고 선형이다. 꽃은 원추화서를 이루며, 양성화는 크고 수술은 5개이며, 자성화는 작고 수술은 퇴화되었으며, 주두가 3개이다. 열매는 포과이며, 종자는 흑갈색의 둥근 난형으로 양명아주는 전초에서 독특한 냄새가 난다. 마트린(matrine)은 양명아주에 약 2% 함유된 알칼로이드 성분으로 고천연 살충성분이다. Chenopodium ambrosioides is an annual plant of the Chenopodium ambrosioides. Yangmyeong aju is a naturalized plant native to South America, and in Korea, it is naturalized throughout the entire region, centering on the Deokcheon River. The stem has many branches, has sparse hairs on the top, and is about 30 to 80 cm tall. Flowers bloom between June and October. The leaves are long, oval-lanceolate, alternate, have teeth of different sizes on the edges, and the petioles are short and have light yellow lines on the back. There are multicellular hairs on the veins, and the upper leaves are linear and without serrations. The flowers form panicles, and the bisexual flowers are large and have 5 stamens, while the unisexual flowers are small, the stamens are degenerated, and have 3 capitals. The fruit is a porpoise, and the seeds are dark brown, round, ovate, and the seedlings of Yangmyeong-Aju have a unique smell. Matrine is an alkaloid contained in about 2% of Yangmyeong root and is a highly natural insecticidal ingredient.
님(Azadirachta indica)은 4천년 전부터 인도에서 해충방제제 많이 사용되어 왔다. 님의 주요성분은 아자디라크틴(azadirachtin)이며, 살라닌(Salannin), 멜리안트리올(Meliantriol) 및 님빈(Nimbin) 성분도 알려져 있다. 특히 아자디라크틴이 주성분인 님오일은 진드기, 응애, 총채벌레, 가루이, 진딧물, 깍지벌레 등의 살충에 효과적이다. 곤충 유충의 탈피를 억제하여 유충 기간이 길어지고 섭식을 방해하는 살충활성이 있어, 1985년 미국 EPA가 생물농약으로 등록하였으며, 중국에서도 2010년에 식물농약으로 등록되었다.Neem (Azadirachta indica) has been widely used as a pest control agent in India since 4,000 years ago. The main component of neem is azadirachtin, and the components Salannin, Meliantriol, and Nimbin are also known. In particular, neem oil, whose main ingredient is azadirachtin, is effective in killing mites, mites, thrips, whiteflies, aphids, and scale insects. It has insecticidal activity that inhibits the molting of insect larvae, prolonging the larval period and interfering with feeding, so it was registered as a biological pesticide by the U.S. EPA in 1985, and was also registered as a botanical pesticide in China in 2010.
본 발명은 파라핀오일, 카란자오일, 계피오일, 정향오일 및 양명아주 추출물을 유효성분으로 사용하고 연막효과를 증가시킬 수 있도록 님오일과 Tween-80을 연막용 확산제로 사용하여 연막제형으로 살포할 경우 닭진드기에 대한 방제활성이 매우 우수한 닭진드기 방제용 연막조성물을 제공하는 것을 목적으로 한다. The present invention uses paraffin oil, caranja oil, cinnamon oil, clove oil, and Yangmyeong root extract as active ingredients, and uses neem oil and Tween-80 as a smoke spreader to increase the smoke screen effect to be sprayed as a smoke screen formulation. The purpose is to provide a smoke screen composition for controlling chicken mites that has excellent control activity against chicken mites.
상기 목적을 달성하기 위하여, 본 발명은 파라핀오일, 카란자오일, 계피오일, 정향오일 및 양명아주 추출물을 유효성분으로 포함하고, 님오일과 Tween-80(Polyoxyethylene Sorbitan Monooleate)을 연막용 확산제로 포함하는, 닭진드기 방제용 연막조성물을 제공한다.In order to achieve the above object, the present invention includes paraffin oil, Carranza oil, cinnamon oil, clove oil, and Yangmyeong root extract as active ingredients, and neem oil and Tween-80 (Polyoxyethylene Sorbitan Monooleate) as a spreader for smoke screen. Provides a smoke screen composition for controlling chicken mites.
상기 조성물은 파라핀오일 15~30중량%, 카란자오일 15~30중량%, 계피오일 5~20중량%, 정향오일 5~20중량%, 양명아주 추출물 10~30중량%, 님오일 5~20중량%, 및 Tween-80 5~10중량%를 포함하는 것이 바람직하다.The composition contains 15-30% by weight of paraffin oil, 15-30% by weight of caranja oil, 5-20% by weight of cinnamon oil, 5-20% by weight of clove oil, 10-30% by weight of Yangmyeong extract, and 5-20% of neem oil. % by weight, and preferably includes 5 to 10 wt% of Tween-80.
상기 조성물은 에탄올 2~5중량%를 더 포함할 수 있다.The composition may further include 2 to 5% by weight of ethanol.
상기 님오일은 분쇄된 님 열매를 90중량% 이상 농도의 에탄올로 2회 이상 추출하는 단계를 포함하는 방법으로 얻는 것이 바람직하다. The neem oil is preferably obtained by a method comprising extracting pulverized neem fruits with ethanol at a concentration of 90% by weight or more two or more times.
또한 본 발명은 파라핀오일 15~30중량%와 카란자오일 15~30중량%를 혼합하는 단계; 파라핀오일과 카란자오일의 혼합물에 계피오일 5~20중량%, 정향오일 5~20중량% 및 양명아주 추출물 10~30중량%를 혼합하는 단계; 및 상기 혼합물에 연막용 확산제로 님오일 5~20중량% 및 Tween-80 5~10중량%를 혼합하는 단계를 포함하는, 닭진드기 방제용 연막조성물의 제조방법을 제공한다.In addition, the present invention includes the steps of mixing 15 to 30% by weight of paraffin oil and 15 to 30% by weight of Carranza oil; Mixing 5 to 20% by weight of cinnamon oil, 5 to 20% by weight of clove oil, and 10 to 30% by weight of Yangmyeong root extract with a mixture of paraffin oil and Carranza oil; and mixing 5 to 20% by weight of neem oil and 5 to 10% by weight of Tween-80 as a smokescreen spreader to the mixture. It provides a method for producing a smokescreen composition for controlling chicken mites.
상기 제조방법에서, 상기 혼합물에 님오일과 Tween-80를 혼합하는 단계에서 에탄올 2~5중량%를 함께 혼합할 수 있다.In the above manufacturing method, 2 to 5% by weight of ethanol may be mixed together in the step of mixing neem oil and Tween-80 in the mixture.
또한 본 발명은 제1항 내지 제3항 중 어느 한 항의 닭진드기 방제용 연막조성물을 계사에 연막처리하는 것을 특징으로 하는 닭진드기의 방제방법을 제공한다.In addition, the present invention provides a method for controlling chicken mites, characterized in that the chicken house is treated with the fogging composition for controlling chicken mites according to any one of claims 1 to 3.
상기 방제방법에서, 상기 닭진드기 방제용 연막조성물은 원액 그대로 사용하거나, 물에 희석하여 사용할 수 있다.In the above control method, the smoke screen composition for controlling chicken mites can be used as a undiluted solution or diluted in water.
상기 방제방법에서, 상기 연막처리는 2주마다 반복하는 것이 바람직하다.In the above control method, it is preferable to repeat the fogging treatment every two weeks.
본 발명의 닭진드기 방제용 연막조성물은 파라핀오일, 카란자오일, 계피오일, 정향오일 및 양명아주 추출물을 유효성분으로 포함하고, 연막효과를 증가시키기 위한 연막용 확산제로 님오일과 Tween-80을 포함하는 것으로, 연막처리를 함으로써 분무 등 종래 처리방법에 비하여 닭진드기에 대한 방제활성이 매우 우수하다. The smoke screen composition for controlling chicken mites of the present invention contains paraffin oil, caranja oil, cinnamon oil, clove oil, and Yangmyeong root extract as active ingredients, and neem oil and Tween-80 as a smoke spreader to increase the smoke screen effect. Including, the smoke film treatment has excellent control activity against chicken mites compared to conventional treatment methods such as spraying.
또한 본 발명의 닭진드기 방제용 연막조성물은 천연 식물추출물을 사용함으로써 인축 및 환경에 영향을 주지 않으면서 닭진드기를 효과적으로 방제할 수 있고, 기존의 살충제 농약과 작용기작이 다르므로 약제저항성 문제도 발생하지 않으며, 잔류농약의 문제도 발생하지 않는 장점이 있다. In addition, the smoke screen composition for controlling chicken mites of the present invention can effectively control chicken mites without affecting livestock and the environment by using natural plant extracts, and has a different mechanism of action from existing pesticides and pesticides, so it also causes problems with drug resistance. It has the advantage of not causing problems with residual pesticides.
따라서 본 발명의 닭진드기 방제용 연막조성물은 계사에 연막처리로 살포함으로써 닭진드기를 효과적으로 방제할 수 있어 닭을 건강하게 키울 수 있다. Therefore, the smoke screen composition for controlling chicken mites of the present invention can effectively control chicken mites by spraying it on chicken houses as a smoke film, allowing chickens to be raised healthily.
도 1은 닭진드기를 채집하여 닭에 접종하는 과정이다.
도 2는 닭진드기를 접종한 닭들에 연막소독으로 본 발명의 닭진드기 방제용 연막조성물을 1차 약제처리하는 과정이다.
도 3은 닭진드기를 접종한 닭들에 연막소독으로 본 발명의 닭진드기 방제용 연막조성물 2차 약제처리하는 과정이다.
도 4는 시험포장 내에 설치된 끈끈이 트랩에 부착된 진드기의 살충율로 본 발명의 닭진드기 방제용 연막조성물의 약효를 조사하는 과정이다.
도 5는 본 발명의 닭진드기 방제용 연막조성물의 실내약효를 조사하는 과정이다.Figure 1 shows the process of collecting chicken mites and inoculating chickens.
Figure 2 shows the process of first chemical treatment with the fogging composition for controlling chicken mites of the present invention by fogging disinfection on chickens inoculated with chicken mites.
Figure 3 shows the process of secondary chemical treatment with the fogging composition for controlling chicken mites of the present invention by fogging disinfection on chickens inoculated with chicken mites.
Figure 4 is a process for investigating the efficacy of the smoke screen composition for controlling chicken mites of the present invention based on the insecticidal rate of ticks attached to a sticky trap installed in the test package.
Figure 5 is a process for investigating the indoor medicinal efficacy of the smoke screen composition for controlling chicken mites of the present invention.
본 발명은 파라핀오일, 카란자오일, 계피오일, 정향오일 및 양명아주 추출물을 유효성분으로 포함하고, 님오일과 Tween-80(Polyoxyethylene Sorbitan Monooleate)을 연막용 확산제로 포함하는, 닭진드기 방제용 연막조성물에 관한 것이다.The present invention is a smoke screen for controlling chicken mites, which contains paraffin oil, caranja oil, cinnamon oil, clove oil, and extract of Yangmyeong Aster as active ingredients, and neem oil and Tween-80 (Polyoxyethylene Sorbitan Monooleate) as a smoke spreader. It relates to composition.
또한 본 발명은 파라핀오일 15~30중량%와 카란자오일 15~30중량%를 혼합하는 단계; 파라핀오일과 카란자오일의 혼합물에 계피오일 5~20중량%, 정향오일 5~20중량% 및 양명아주 추출물 10~30중량%를 혼합하는 단계; 및 상기 혼합물에 연막용 확산제로 님오일 5~20중량% 및 Tween-80 5~10중량%를 혼합하는 단계를 포함하는, 닭진드기 방제용 연막조성물의 제조방법에 관한 것이다.In addition, the present invention includes the steps of mixing 15 to 30% by weight of paraffin oil and 15 to 30% by weight of Carranza oil; Mixing 5 to 20% by weight of cinnamon oil, 5 to 20% by weight of clove oil, and 10 to 30% by weight of Yangmyeong root extract with a mixture of paraffin oil and Carranza oil; and mixing 5 to 20% by weight of neem oil and 5 to 10% by weight of Tween-80 as a smokescreen spreader to the mixture. It relates to a method for producing a smokescreen composition for controlling chicken mites.
또한 본 발명은 닭진드기 방제용 연막조성물을 계사에 연막처리하는 것을 특징으로 하는 닭진드기의 방제방법에 관한 것이다.In addition, the present invention relates to a method for controlling chicken mites, characterized in that the chicken house is treated with a fogging composition for controlling chicken mites.
이하 본 발명을 상세하게 설명한다.Hereinafter, the present invention will be described in detail.
1. 식물추출물1. Plant extract
식물추출물로는 카란자오일, 계피오일, 정향오일 및 양명아주 추출물을 사용한다. Plant extracts include Carranza oil, cinnamon oil, clove oil, and Yangmyeong root extract.
카란자, 계피, 정향 및 양명아주는 재배한 것이나 시판되는 것을 제한없이 사용할 수 있다. Karanja, cinnamon, cloves, and lamb seedlings can be used without restrictions, whether cultivated or commercially available.
식물추출물의 추출방법으로는 여과법, 열수추출, 침지추출, 환류냉각추출, 초음파추출 등 통상적으로 사용되는 추출방법을 사용할 수 있다.Commonly used extraction methods such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction can be used as extraction methods for plant extracts.
(1) 카란자오일(1) Carranza oil
카란자오일은 카란자 씨앗으로부터 오일성분을 추출한 것으로 주성분은 카라진이다. 카란자오일은 용매추출법 등 통상적인 추출방법으로 제조하여 사용하거나, 상업적으로 구입가능한 것을 사용한다. Carranza oil is an oil extracted from Carranza seeds, and its main ingredient is carragine. Carranza oil can be manufactured and used using conventional extraction methods such as solvent extraction, or commercially available oil can be used.
카란자오일은 카란자 씨앗을 세척하여 껍질을 제거한 다음 100~120℃ 정도로 가열하여 물리적으로 압착한 후 여과하는 비정제유 냉압착추출법을 이용하여 제조하는 것이 바람직하다.Carranza oil is preferably produced using the unrefined cold-pressed extraction method, which involves washing Carranza seeds, removing the skin, heating to about 100-120°C, physically pressing, and then filtering.
추출된 카란자오일은 물에 녹지 않으므로 카란자오일과 계면활성제를 18~22:1의 중량비로 혼합하여 카란자오일을 수용성 형태로 제조하여 사용하는 것이 바람직하다. 이때 계면활성제로는 Tween-80 계면활성제를 사용하는 것이 바람직하다.Since extracted Carranza oil is not soluble in water, it is preferable to prepare Carranza oil in a water-soluble form by mixing Carranza oil and a surfactant at a weight ratio of 18 to 22:1. At this time, it is preferable to use Tween-80 surfactant as the surfactant.
(2) 계피오일(2) Cinnamon oil
계피오일은 계피로부터 오일성분을 추출한 것으로, 주성분은 시남알데하이드이다. 계피오일은 열수추출법, 가압추출물, 용매추출법 등 통상적인 추출방법으로 제조하여 사용하거나, 상업적으로 구입가능한 것을 사용한다.Cinnamon oil is an oil component extracted from cinnamon, and its main component is cinnamaldehyde. Cinnamon oil can be manufactured and used using conventional extraction methods such as hot water extraction, pressurized extraction, and solvent extraction, or use commercially available products.
계피오일을 추출할 때 에탄올을 이용한 용매추출법이 수율이 가장 높다. 에탄올을 이용하여 계피오일을 추출하는 경우, 계피와 70중량% 에탄올을 1:2~4의 비율(w/v)로 혼합하여 45~50시간 동안 추출하고 고형분을 걸러낸 다음 감압여과하여 계피오일성분을 추출하는 것이 바람직하다. When extracting cinnamon oil, solvent extraction using ethanol has the highest yield. When extracting cinnamon oil using ethanol, mix cinnamon and 70% by weight ethanol at a ratio (w/v) of 1:2 to 4, extract for 45 to 50 hours, filter out the solids, and then filter under reduced pressure to obtain cinnamon oil. It is desirable to extract the ingredients.
(3) 정향오일(3) Clove oil
정향오일은 정향으로부터 오일성분을 추출한 것으로, 주성분은 유제놀이다. 정향오일은 정향의 건조시킨 꽃봉오리, 꽃자루 및 잎을 수증기로 증류하여 제조하거나, 상업적으로 구입가능한 것을 사용한다.Clove oil is an oil component extracted from cloves, and its main component is eugenol. Clove oil is manufactured by steam distillation of dried flower buds, peduncles and leaves of cloves, or commercially available oil is used.
정향오일는 정향을 플라스크에 담고 다른 플라스크에는 정향의 중량과 1:0.8~1.2의 비율(w/v)로 헥산을 넣고 두 개의 플라스크를 냉각수로 연결한 후 가열하여 5~6시간 동안 환류시키고, 환류 후 서서히 냉각시켜 헥산이 담긴 플라스크를 분리하여 탈수한 다음 여과하고, 여과액을 25℃에서 증발시켜 무색의 용액상태인 정향오일을 제조하는 것이 바람직하다. For clove oil, put cloves in a flask, add hexane in a ratio (w/v) of 1:0.8 to 1.2 to the weight of cloves in another flask, connect the two flasks with cooling water, heat and reflux for 5 to 6 hours, and reflux. Afterwards, it is preferable to gradually cool the flask containing the hexane, dehydrate it, filter it, and evaporate the filtrate at 25°C to produce clove oil in a colorless solution.
(4) 양명아주 추출물(4) Yangmyeong root extract
양명아주 추출물의 주요성분은 마트린이며, 양명아주 추출물에는 마트린이 0.1~0.2중량% 함유되어 있다. 양명아주 추출물은 공지의 추출방법을 사용하여 제조하거나, 정제된 마트린 성분을 구입하여 사용할 수도 있다.The main component of Yangmyeong agar root extract is matrine, and the Yangmyeong agar root extract contains 0.1 to 0.2% by weight of matrine. Yangmyeong soybean extract can be prepared using a known extraction method, or it can be used by purchasing a purified matrine component.
양명아주 추출물은 양명아주를 세척한 후 분쇄하고, 양명아주 분쇄물에 추출용매를 1~2:1의 중량비로 혼합하여 상온에서 7~10시간 동안 추출하고, 추출물을 농축하여 제조한다. 추출용매로는 물과 에탄올을 혼합하여 사용하는 것이 바람직하며, 물과 에탄올을 1:1~3의 중량비로 혼합하여 사용하는 것이 보다 바람직하다.Yangmyeong agar root extract is prepared by washing and pulverizing the Yangmyeong agar root, mixing the pulverized Yangmyeong agar root with an extraction solvent at a weight ratio of 1 to 2:1, extracting at room temperature for 7 to 10 hours, and concentrating the extract. It is preferable to use a mixture of water and ethanol as an extraction solvent, and it is more preferable to use a mixture of water and ethanol at a weight ratio of 1:1 to 3.
마트린은 다른 살충효과를 가진 물질과 혼합하여 사용할 때 해충의 구제에 상승작용을 나타내어 적은 양을 사용하더라도 우수한 해충 구제효과가 있다. Matrine has a synergistic effect in exterminating pests when mixed with other substances with insecticidal effects, so even when used in small amounts, it has an excellent pest extermination effect.
2. 파라핀오일2. Paraffin oil
파라핀 오일은 시판제품을 구입하여 사용한다. 정유회사에서 유기농허용물질인 파라핀오일을 구입하여 사용하는 것이 바람직하다.Paraffin oil is purchased and used commercially. It is advisable to purchase and use paraffin oil, which is an organically permitted substance, from an oil refinery company.
3. 연막용 확산제3. Spreading agent for smoke screen
연막조성물을 제조하기 위한 연막용 확산제로 님오일과 Tween-80(Polyoxyethylene Sorbitan Monooleate)을 사용한다.Neem oil and Tween-80 (Polyoxyethylene Sorbitan Monooleate) are used as diffusion agents for smoke screens to produce smoke screen compositions.
님오일은 님식물로부터 오일성분을 추출한 것으로, 상업적으로 구입가능한 것을 사용하거나 제조하여 사용할 수 있다. 바람직하게는 다음과 같은 방법으로 제조하여 사용할 수 있다.Neem oil is an oil component extracted from the neem plant, and can be used commercially or manufactured. Preferably, it can be manufactured and used by the following method.
먼저 님 열매를 분쇄한다. 분쇄된 님 열매를 90중량% 이상 농도의 에탄올로 2회 이상 추출하는 것이 바람직하다. 더욱 바람직하게는 추출은 3회 반복하는 것이 좋다. 추출된 님오일을 감압건조하여 님오일 분말을 얻는다. 얻어진 님오일 분말을 정제수에 용해시켜 사용한다. First, crush the neem fruit. It is desirable to extract the crushed neem fruit at least twice with ethanol at a concentration of 90% by weight or more. More preferably, the extraction is repeated three times. Neem oil powder is obtained by drying the extracted neem oil under reduced pressure. The obtained neem oil powder is dissolved in purified water and used.
조성물 중에 님오일은 5~20중량%, Tween-80은 5~10중량%로 포함되는 것이 바람직하다.It is preferable that neem oil is contained in an amount of 5 to 20% by weight and Tween-80 is contained in an amount of 5 to 10% by weight.
연막용 확산제는 필요에 따라 에탄올을 더 포함할 수 있다. 에탄올은 조성물의 유동성을 높여줄 수 있다. 에탄올을 사용하는 경우, 에탄올은 조성물 중 2~5중량%를 사용하는 것이 바람직하다.The smoke spreader may further contain ethanol as needed. Ethanol can increase the fluidity of the composition. When using ethanol, it is desirable to use 2 to 5% by weight of the composition.
연막용 확산제를 사용함으로써 연막살포를 할 수 있어 종래 분무 등의 방법에 비해 닭진드기를 효과적으로 방제할 수 있다.By using a smoke spreader, smoke screen can be sprayed and chicken mites can be controlled more effectively than conventional spraying methods.
4. 닭진드기 방제용 연막조성물4. Smoke screen composition for chicken mite control
유효성분으로 파라핀오일, 카란자오일, 계피오일, 정향오일 및 양명아주 추출물을 혼합하여 사용하고, 연막용 확산제로 님오일과 Tween-80을 사용한다.A mixture of paraffin oil, caranja oil, cinnamon oil, clove oil, and Yangmyeong root extract is used as active ingredients, and neem oil and Tween-80 are used as a smoke spreading agent.
조성물 중 파라핀오일은 15~30중량%, 카란자오일은 15~30중량%, 계피오일은 5~20중량%, 정향오일은 5~20중량% 및 양명아주 추출물은 10~30중량%를 사용하는 것이 바람직하고, 님오일은 5~20중량%, Tween-80은 5~10중량%를 사용하는 것이 바람직하다. In the composition, paraffin oil is used at 15-30% by weight, Carranza oil is used at 15-30% by weight, cinnamon oil is used at 5-20% by weight, clove oil is used at 5-20% by weight, and Yangmyeong root extract is used at 10-30% by weight. It is preferable to use 5 to 20% by weight of neem oil and 5 to 10% by weight of Tween-80.
먼저, 파라핀오일과 카란자오일을 혼합한다. 카란자 오일과 파라핀 오일의 혼합물에 계피오일, 정향오일 및 양명아주 추출물을 혼합한다.First, mix paraffin oil and Carranza oil. Mix cinnamon oil, clove oil, and Yangmyeong root extract into a mixture of Carranza oil and paraffin oil.
상기 혼합물에 연막용 확산제로 님오일과 Tween-80을 혼합하며, 필요에 따라 에탄올을 함께 혼합한다. 에탄올을 더 포함하는 경우 에탄올은 조성물 중 2~5중량%를 사용하는 것이 바람직하다.In the above mixture, neem oil and Tween-80 are mixed as a smoke spreading agent, and ethanol is mixed as necessary. When ethanol is further included, it is preferable to use 2 to 5% by weight of ethanol in the composition.
상기와 같이 제조된 닭진드기 방제용 연막조성물은 제조 후 2개월이 경과하여도 분산된 조성물의 층이 분리되지 않아 경시변화가 없다.The smoke screen composition for controlling chicken mites prepared as described above shows no change over time because the layers of the dispersed composition are not separated even after 2 months of production.
따라서, 본 발명의 닭진드기 방제용 연막조성물은 원액 그대로 사용하거나, 물에 희석하여 사용할 수 있다. 물에 희석하여 사용하는 경우, 2~1,000배 희석하여 사용하는 것이 바람직하다. 물에 1,000배 희석하여 사용한 경우에도, 최종약제 처리 후 96시간 이내 진드기에 대하여 100% 방제효과를 보였다.Therefore, the smoke screen composition for controlling chicken mites of the present invention can be used as a undiluted solution or diluted in water. When diluted in water and used, it is recommended to dilute 2 to 1,000 times. Even when diluted 1,000 times in water, it showed a 100% control effect against ticks within 96 hours after the final treatment.
본 발명의 닭진드기 방제용 연막조성물은 계사에 연막처리로 처리하여 닭진드기를 방제한다. The smoke screen composition for controlling chicken mites of the present invention controls chicken mites by treating chicken houses with smoke screen treatment.
본 발명의 닭진드기 방제용 연막조성물의 효과는 2주 정도 지속되므로 2주 마다 반복하여 연막처리하는 것이 바람직하다.Since the effect of the fogging composition for controlling chicken mites of the present invention lasts for about two weeks, it is preferable to repeat fogging treatment every two weeks.
이하 실시예를 통하여 본 발명을 상세하게 설명한다. 이들 실시예는 본 발명을 예시하는 것으로서 본 발명의 범위가 이에 한정되는 것은 아니다.The present invention will be described in detail below through examples. These examples illustrate the present invention and the scope of the present invention is not limited thereto.
<제조예 1> <Manufacturing Example 1>
카란자오일의 제조 Manufacturing of Carranza oil
카란자 씨앗을 세척하고 껍질을 제거한 다음 100~120℃의 온도로 가열하면서 물리적 압착을 하고 여과하여 카란자오일을 추출하였다. 추출된 카란자오일에 Tween-80 계면활성제를 20:1의 중량비로 혼합하여 추출액을 수용성으로 조제하였다.Carranza seeds were washed and peeled, then physically pressed and filtered while heated to a temperature of 100-120°C to extract Carranza oil. The extracted Carranza oil was mixed with Tween-80 surfactant at a weight ratio of 20:1 to prepare a water-soluble extract.
<제조예 2> <Production Example 2>
계피오일의 제조Production of cinnamon oil
계피 250g에 70% 에탄올 750㎖를 혼합하고 수조에서 48시간 동안 처리하여 용출시켰다. 고형분을 걸러낸 다음 감압 여과장치를 이용하여 계피오일을 추출하였다. 250 g of cinnamon was mixed with 750 ml of 70% ethanol and treated in a water bath for 48 hours to elute. After filtering out the solids, cinnamon oil was extracted using a reduced pressure filtration device.
<제조예 3> <Production Example 3>
정향오일의 제조Manufacturing of clove oil
50g의 정향의 눈(bud)과 잎을 건조시켜 절단하고 500㎖ 플라스크에 가한 후 증류수를 넣었다. 다른 플라스크에는 50㎖의 헥산을 넣어 두 개의 플라스크를 냉각수로 연결한 후 가열하여 5시간 동안 환류시켰다. 환류 후 다시 서서히 냉각시켜 헥산이 담긴 플라스크를 분리하여 탈수한 다음 여과하였다. 여과액을 25℃에서 증발시켜 무색의 용액상태인 정향오일을 제조하였다.50g of clove buds and leaves were dried, cut, added to a 500ml flask, and then distilled water was added. 50 ml of hexane was added to another flask, the two flasks were connected with cooling water, and then heated and refluxed for 5 hours. After refluxing, it was slowly cooled again, and the flask containing hexane was separated, dehydrated, and then filtered. The filtrate was evaporated at 25°C to prepare clove oil in a colorless solution.
<제조예 4> <Production Example 4>
양명아주 추출물의 제조Preparation of Yangmyeong root extract
양명아주 900g을 세척한 후 분쇄하였다. 양명아주 분쇄물에 물 200g과 에탄올 400g을 넣은 후 상온에서 8시간 동안 추출하였다. 얻어진 추출물을 에탄올 농도가 2%가 되도록 농축하여 양명아주 추출물을 제조하였다.900g of Yangmyeong root was washed and ground. 200 g of water and 400 g of ethanol were added to the pulverized material of Yangmyeong root and extracted for 8 hours at room temperature. The obtained extract was concentrated to an ethanol concentration of 2% to prepare a Yangmyeong root extract.
<제조예 5> <Production Example 5>
님오일의 제조Production of neem oil
님열매 1kg를 분쇄하고 분쇄된 님열매를 95중량% 에탄올을 사용하여 3회 반복 추출하였다. 추출물을 감압건조시켜 님오일 분말 150g을 얻었다. 얻어진 님오일 분말을 정제수 1ℓ에 용해시켰다. 추출물 중 아자디라크틴의 함량은 0.22%이었다.1 kg of neem fruit was crushed, and the crushed neem fruit was extracted three times using 95% by weight ethanol. The extract was dried under reduced pressure to obtain 150 g of neem oil powder. The obtained neem oil powder was dissolved in 1 liter of purified water. The content of azadirachtin in the extract was 0.22%.
<실시예 1><Example 1>
닭진드기 방제용 연막조성물의 제조Manufacture of smoke screen composition for chicken mite control
카란자오일 25g, 파라핀오일(P20, 하나 C&G) 20g, 정향오일 10g, 계피오일 10g 및 양명아주 추출물 20g을 혼합하였다. 이 혼합물에 연막용 확산제로 님오일 10g, Tween-80 7g 및 에탄올 3g을 혼합하여 닭진드기 방제용 연막조성물을 제조하였다. 25g of Carranza oil, 20g of paraffin oil (P20, Hana C&G), 10g of clove oil, 10g of cinnamon oil, and 20g of Yangmyeong root extract were mixed. A smoke screen composition for chicken mite control was prepared by mixing 10 g of neem oil, 7 g of Tween-80, and 3 g of ethanol as a smoke spreader to this mixture.
<실시예 2><Example 2>
닭진드기 방제용 연막조성물의 제조Manufacture of smoke screen composition for chicken mite control
카란자오일 20g, 파라핀오일(P20, 하나 C&G) 25g, 정향오일 10g, 계피오일 10g 및 양명아주 추출물 20g을 혼합하였다. 이 혼합물에 연막용 확산제로 님오일 10g, Tween-80 7g 및 에탄올 3g을 혼합하여 닭진드기 방제용 연막조성물을 제조하였다. 20g of Carranza oil, 25g of paraffin oil (P20, Hana C&G), 10g of clove oil, 10g of cinnamon oil, and 20g of Yangmyeong root extract were mixed. A smoke screen composition for chicken mite control was prepared by mixing 10 g of neem oil, 7 g of Tween-80, and 3 g of ethanol as a smoke spreader to this mixture.
<실시예 3><Example 3>
닭진드기 방제용 연막조성물의 제조Manufacture of smoke screen composition for chicken mite control
카란자오일 20g, 파라핀오일(P20, 하나 C&G) 20g, 정향오일 10g, 계피오일 10g 및 양명아주 추출물 20g을 혼합하였다. 이 혼합물에 연막용 확산제로 님오일 10g, Tween-80 7g 및 에탄올 3g을 혼합하여 닭진드기 방제용 연막조성물을 제조하였다. 20g of Carranza oil, 20g of paraffin oil (P20, Hana C&G), 10g of clove oil, 10g of cinnamon oil, and 20g of Yangmyeong root extract were mixed. A smoke screen composition for chicken mite control was prepared by mixing 10 g of neem oil, 7 g of Tween-80, and 3 g of ethanol as a smoke spreader to this mixture.
<실험예 1><Experimental Example 1>
닭진드기 방제용 연막조성물의 약효조사Investigation of the efficacy of smoke screen compositions for controlling chicken mites
본 발명의 닭진드기 방제용 연막조성물의 약효를 다음과 같이 조사하였다.The efficacy of the smoke screen composition for controlling chicken mites of the present invention was investigated as follows.
실험은 충북 오창 시험포장에서 실시하였고, 시험약제로는 실시예 3의 닭진드기 방제용 연막조성물을 사용하였다. 실험구 1은 실시예 3의 닭진드기 방제용 연막조성물을 4배 희석하여 처리한 것, 실험구 2는 실시예 3의 닭진드기 방제용 연막조성물을 2배 희석하여 처리한 것, 실험구 3은 실시예 3의 닭진드기 방제용 연막조성물을 원액 그대로 처리한 것이다. 시험약제는 물을 사용하여 희석하였다.The experiment was conducted at a test field in Ochang, North Chungcheong Province, and the smoke screen composition for controlling chicken mites of Example 3 was used as the test agent. Experimental group 1 was treated by diluting the smoke film composition for controlling chicken mites of Example 3 by 4 times, experimental group 2 was treated by diluting the smoke film composition for controlling chicken mites of Example 3 by 2 times, and experimental group 3 was treated by diluting the smoke film composition for controlling chicken mites of Example 3 by 2 times. The smoke screen composition for controlling chicken mites of Example 3 was treated as a undiluted solution. The test drug was diluted with water.
약효를 비교하기 위한 비교구로 연막조성물을 처리하지 않은 무처리구를 사용하였다.An untreated group that was not treated with the smoke screen composition was used as a comparative tool to compare drug efficacy.
먼저, 도 1에서와 같이 닭진드기를 채집하고, 시험포장인 양계사 전체에 채집한 닭진드기 약 1000마리를 골고루 접종하였다. 도 2에서와 같이 닭진드기를 접종한 양계사에 연막소독으로 1차 약제처리를 실시하였다. 다음으로 도 3에서와 같이 연막소독으로 2차 약제처리를 실시하였다. 약제처리 후 도 4와 같이 각 처리구 상단에 노란색 끈끈이 트랩을 설치하고 트랩에 부착된 닭진드기 포집마리수를 확인하여 본 발명의 닭진드기 방제용 연막조성물의 약효를 조사하였다. First, chicken mites were collected as shown in Figure 1, and about 1,000 collected chicken mites were evenly inoculated throughout the poultry house, which was the test field. As shown in Figure 2, the first chemical treatment was performed on the poultry house inoculated with chicken mites by fogging disinfection. Next, as shown in FIG. 3, secondary chemical treatment was performed through fogging disinfection. After chemical treatment, a yellow sticky trap was installed at the top of each treatment area as shown in Figure 4, and the number of chicken mites captured attached to the trap was confirmed to investigate the effectiveness of the smoke screen composition for controlling chicken mites of the present invention.
상기 실험은 처리구당 5회 반복하였고, 그 결과를 하기 표 1에 나타내었다.The experiment was repeated five times per treatment group, and the results are shown in Table 1 below.
실험결과, 연막조성물을 처리하지 않은 무처리구에서는 평균 6~8마리의 닭진드기가 포집되었고, 연막조성물을 처리한 실험구들에서는 실험구 및 실험회차별 차이는 있었지만 0~2마리의 닭진드기가 포집되었다. As a result of the experiment, an average of 6 to 8 chicken mites were collected in the untreated areas that were not treated with the smoke composition, and in the experimental areas treated with the smoke composition, 0 to 2 chicken mites were collected, although there were differences depending on the experimental group and experiment period. .
(무처리구-처리구)/무처리구×100로 계산하는 방제가 산출공식(농약시험과 방법: 농진청고시)에 따라 방제가를 산출하여 평균한 결과, 본 발명의 닭진드기 방제용 연막조성물의 방제효과는 평균 89.3%이었다.As a result of calculating the control value as (untreated area - treated area) / untreated area × 100, the control value was calculated and averaged according to the calculation formula (Pesticide Test and Method: Rural Development Administration Notice), the control effect of the smoke screen composition for controlling chicken mites of the present invention was averaged. It was 89.3%.
<실험예 2><Experimental Example 2>
닭진드기 방제용 연막조성물의 희석농도에 따른 방제효과Control effect according to dilution concentration of smoke screen composition for controlling chicken mites
본 발명의 닭진드기 방제용 연막조성물의 희석농도에 따른 닭진드기 방제효과를 확인하기 위하여 실내 실험실에서 농도별로 희석한 약제를 처리하여 닭진드기 방제효과를 경시적으로 조사하였다.In order to confirm the chicken mite control effect according to the diluted concentration of the chicken mite control smoke composition of the present invention, the chicken mite control effect was investigated over time by treating the drug diluted by concentration in an indoor laboratory.
진드기를 채집하고, 채집한 진드기는 시험전 30±3℃의 온도, 50~80%의 상대습도, 명:암=16시간:8시간의 광주기 조건으로 보관하였다. Ticks were collected, and the collected mites were stored at a temperature of 30 ± 3°C, relative humidity of 50 to 80%, and photoperiod of light: dark = 16 hours: 8 hours before testing.
시험약제로는 실시예 1과 실시예 2의 닭진드기 방제용 연막조성물을 사용하였다. 각 시험약제의 처리에서 처리 1은 10배 희석하여 처리한 것, 처리 2는 100배 희석하여 처리한 것, 처리 3은 500배 희석하여 처리한 것, 처리 4는 1,000배 희석하여 처리한 것이다. 시험약제는 물을 사용하여 희석하였다.As test agents, the chicken mite control smoke compositions of Examples 1 and 2 were used. In the treatment of each test drug, treatment 1 was diluted 10 times, treatment 2 was diluted 100 times, treatment 3 was diluted 500 times, and treatment 4 was diluted 1,000 times. The test drug was diluted with water.
1회 실험당 30마리의 진드기를 사용하였고, 약제처리 후 12시간, 24시간, 48시간, 72시간, 96시간 및 120시간 후 진드기의 생존율을 조사하였다. 약효를 비교하기 위한 비교구로 연막조성물을 처리하지 않은 무처리구를 사용하였다. 채집된 진드기는 시험공시 7일 후 재확산을 우려하여 즉시 폐기하였다. 30 ticks were used per experiment, and the survival rate of ticks was examined 12 hours, 24 hours, 48 hours, 72 hours, 96 hours, and 120 hours after drug treatment. An untreated group that was not treated with the smoke screen composition was used as a comparative tool to compare drug efficacy. The collected mites were immediately discarded 7 days after the test announcement due to concerns about re-proliferation.
본 발명의 닭진드기 방제용 연막조성물의 희석농도에 따른 실내에서의 닭진드기 방제효과를 조사한 결과를 하기 표 2에 나타내었다.The results of investigating the effect of controlling chicken mites indoors according to the dilution concentration of the smoke screen composition for controlling chicken mites of the present invention are shown in Table 2 below.
접종마리수per repetition
Number of inoculated animals
실내실험에서, 본 발명의 닭진드기 방제용 연막조성물은 약제처리 후 96시간 이내에는 모든 희석농도에서 진드기에 대하여 100% 방제효과를 보였다. 그러나, 2주 후 진드기가 다시 소량 발생하는 것으로 확인되었다.In an indoor experiment, the smoke screen composition for controlling chicken mites of the present invention showed a 100% control effect against mites at all diluted concentrations within 96 hours after chemical treatment. However, after two weeks, it was confirmed that mites appeared again in small quantities.
따라서, 본 발명의 닭진드기 방제용 연막조성물은 2주마다 반복하여 처리하는 것이 바람직하다.Therefore, it is preferable to repeat treatment with the smoke screen composition for controlling chicken mites of the present invention every two weeks.
Claims (8)
님오일 5~20중량%와 Tween-80(Polyoxyethylene Sorbitan Monooleate) 5~10중량%를 연막용 확산제로 포함하는, 닭진드기 방제용 연막조성물.Contains 15-30% by weight of paraffin oil, 15-30% by weight of Carranza oil, 5-20% by weight of cinnamon oil, 5-20% by weight of clove oil, and 10-30% by weight of Yangmyeong root extract as active ingredients. Jaoil is manufactured in a water-soluble form by mixing Carranza oil with a surfactant at a weight ratio of 18 to 22:1.
A smoke screen composition for controlling chicken mites, comprising 5 to 20% by weight of neem oil and 5 to 10% by weight of Tween-80 (Polyoxyethylene Sorbitan Monooleate) as a smoke spreader.
상기 카란자오일은 카란자 씨앗을 100~120℃로 가열하고 물리적으로 압착한 후 여과하여 얻은 추출물 형태의 카란자 오일에 계면활성제를 혼합하여 수용성 형태로 제조한 것임을 특징으로 하는, 닭진드기 방제용 연막조성물.According to paragraph 1,
The Carranza oil is for controlling chicken mites, characterized in that it is prepared in a water-soluble form by mixing surfactant with Carranza oil in the form of an extract obtained by heating Carranza seeds to 100-120°C, physically compressing, and then filtering. Smoke composition.
상기 조성물은 에탄올 2~5중량%를 더 포함하는 것을 특징으로 하는 닭진드기 방제용 연막조성물.According to paragraph 1,
The composition is a smoke screen composition for controlling chicken mites, characterized in that it further contains 2 to 5% by weight of ethanol.
상기 님오일은 분쇄된 님 열매를 90중량% 이상 농도의 에탄올로 2회 이상 추출하는 단계를 포함하는 방법으로 얻는 것을 특징으로 하는 닭진드기 방제용 연막조성물.According to paragraph 1,
The neem oil is a fogging composition for controlling chicken mites, characterized in that it is obtained by a method comprising extracting pulverized neem fruits with ethanol at a concentration of 90% by weight or more two or more times.
상기 단계에서 얻은 수용성 형태의 카란자오일 15~30중량%와 파라핀오일 15~30중량%를 혼합하는 단계;
상기 단계에서 얻은 파라핀오일과 카란자오일의 혼합물에 계피오일 5~20중량%, 정향오일 5~20중량% 및 양명아주 추출물 10~30중량%를 혼합하여 혼합물을 얻는 단계; 및
상기 단계에서 얻은 혼합물에 연막용 확산제로 님오일 5~20중량% 및 Tween-80 5~10중량%를 혼합하는 단계를 포함하는, 닭진드기 방제용 연막조성물의 제조방법.Preparing Carranza oil in a water-soluble form by mixing Carranza oil with a surfactant at a weight ratio of 18 to 22:1;
Mixing 15 to 30% by weight of water-soluble Carranza oil obtained in the above step and 15 to 30% by weight of paraffin oil;
Obtaining a mixture by mixing 5 to 20% by weight of cinnamon oil, 5 to 20% by weight of clove oil, and 10 to 30% by weight of Yangmyeong root extract with the mixture of paraffin oil and Carranza oil obtained in the above step; and
A method for producing a smoke screen composition for controlling chicken mites, comprising mixing 5 to 20% by weight of neem oil and 5 to 10% by weight of Tween-80 as a smoke screen spreader with the mixture obtained in the above step.
상기 혼합물에 님오일과 Tween-80를 혼합하는 단계에서 에탄올 2~5중량%를 함께 혼합하는 것을 특징으로 하는, 닭진드기 방제용 연막조성물의 제조방법.According to clause 5,
A method for producing a smoke screen composition for controlling chicken mites, characterized in that 2 to 5% by weight of ethanol is mixed together in the step of mixing neem oil and Tween-80 in the mixture.
상기 연막처리는 상기 연막조성물을 원액 또는 2~1,000배 희석하여 2주마다 반복하는 것을 특징으로 하는 닭진드기의 방제방법.
In clause 7,
The smoke screen treatment is a method for controlling chicken mites, characterized in that the smoke screen composition is diluted as a raw solution or 2 to 1,000 times and repeated every two weeks.
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