KR100835610B1 - Compositions for controlling pests comprising plantextracts as effective components - Google Patents

Abstract

A vegetable natural pest controlling composition is provided to be harmless to human and stock by selectively acting on harmful insects and pathogens doing harm on a plant and being decomposed naturally by the passage of time and control the harmful insects with resistance against chemical pesticide efficiently and economically. A method for preparing a vegetable natural pest controlling composition comprises the steps of: (a) preparing an extract of Melia azadirachta; (b) leaving poroligenous liquor extracted from waste wood or material lumber using a carbonizer for 6 months to 1 year to prepare the poroligenous liquor; (c) preparing an extract of Chrysanthemum cinerariaefolium; and (d) mixing the extract of Melia azadirachta, the poroligenous liquor and the extract of Chrysanthemum cinerariaefolium in a volumetric ratio of 1:1:1 to 9:1:1, wherein the extract of Melia azadirachta is prepared by: (a-1) after putting 100g of crushed nucleus of Melia azadirachta fruits in a circular flask and putting 150ml of methanol therein, leaving it at room temperature for 3 days; (a-2) after putting the flask on a heating mantle and heating it at a temperature of 75 deg.C, recovering the evaporated methanol using a cooling device; (a-3) repeating the steps(a-1) and (a-2) at least three times to extract the Melia azadirachta enough; (a-4) after filtering the extract using a filter paper, removing the methanol therefrom using an evaporator to prepare dried Melia azadirachta powder; and (a-5) after dissolving 10g of the powder in 25ml of ethanol, adding 75ml of Tween80 and 400ml of water thereto to prepare the Melia azadirachta extract, and the extract of Chrysanthemum cinerariaefolium is prepared by: (c-1) after putting 100g of crushed flower bud of nucleus of Chrysanthemum cinerariaefolium in a circular flask and putting 150ml of methanol therein, leaving it at room temperature for 3 days; (c-2) after putting the flask on a heating mantle and heating it at a temperature of 75 deg.C, recovering the evaporated methanol using a cooling device; (c-3) repeating the steps(c-1) and (c-2) at least three times to extract the Chrysanthemum cinerariaefolium enough; (c-4) after filtering the extract using a filter paper, removing the methanol therefrom using an evaporator to prepare dried Chrysanthemum cinerariaefolium powder; and (c-5) after dissolving 10g of the powder in 25ml of ethanol, adding 75ml of Tween80 and 400ml of water thereto to prepare the Chrysanthemum cinerariaefolium extract.

Description

Vegetable natural pest control composition and its manufacturing method {COMPOSITIONS FOR CONTROLLING PESTS COMPRISING PLANTEXTRACTS AS EFFECTIVE COMPONENTS}

The invention of vegetable natural pest control that the natural extracts as the active ingredient composition and using the same disinfection relates to insecticidal effect, and more particularly's extract of (Azadirachta indica) and vinegar (pyroligneous liquid), pyrethrum (Chrysanthemum cinerariaefolium) The present invention relates to a suitable plant natural pest control composition and a method for producing the same, which are harmless to the human body in combination, do not harm plant growth, protect crops from pests and have insecticidal bactericidal effects on many pests.

To date, a variety of products are being marketed as organic pesticides in agriculture, skins, essences, and pet shampoos, using the oil and cakes of nim trees containing Azadirachtin.

Azadirachtin was officially approved as a biopesticide by the US Environmental Protection Agency in 1985, and in 1988 a market of more than $ 100 billion was formed. In 2000, the United Nations already declared it a plant of the 21st century, proclaiming it as an alternative to the problem of global pests.

Neem tree is not only used to solve problems related to pests, but also covers cancer, heart disease, herpes, periodontal disease, skin disease, allergy, gastric ulcer, duodenal ulcer, antifungal, malaria, pest repellent, etc. The research to be developed is ongoing and has many achievements.

The pyrethrin also has an insecticidal disinfecting effect. Especially, the pyrethrin product is also known as pyreth in Korea and is used for eco-friendly agriculture, organic farming and mosquito medicine.

Wood vinegar has an insecticidal effect, and it is a food of useful microorganisms, and it is able to multiply organisms. It is rich in organic acids such as acetic acid, and it dissolves various substances well. Alcohols such as methanol and prephenol, ketones, aldehydes, etc. It acts to make molecules smaller, increasing their penetration and absorption into plants. In addition, as a physiologically active substance of plants, the reaction proceeds smoothly in the crop.

The above plant extracts have been used as insecticides as follows.

Korean Patent Publication No. 2006-71380 consists of wormwood extract, red ginseng extract, soybean extract, sesame oil, and penetrant, and if necessary, Neem oil (Mushle oil), Algae extract, White poplar extract, White cedar extract, Castor extract Natural insecticides are described as biopesticides using plant extracts further comprising

In Korean Patent Registration No. 10-596 (1960.2.2), insecticide supplements such as linden, nicotine, dentiti, pesticides and fragrance disinfectants, and sodium bicarbonate, petroleum and ether are mixed and placed in the container. The liquid is described,

Korean Patent Registration No. 10-278796 (2000.10.24) discloses wood vinegar extract, lysocithin, pesticides containing fatty acids and wood vinegar composition for promoting growth,

Korean Patent No. 96-13053 discloses a hydrogenated pestle extract having insecticidal properties by extracting seeds of hydrogenated pestle,

Conventional techniques as described above are related to biopesticides using plant extracts, but have not been used in practice due to insecticidal problems, or have been used only in some trials.

In order to minimize or solve the problems as described above, the present invention is to prepare a composition having excellent insecticidal, bactericidal power to more kinds of pests by mixing the Karanja tree known as natural insecticides such as Neem trees and wormwood, wood vinegar solution This is to maximize the productivity of agriculture using natural extracts, not chemicals.

The present invention comprises a combination of Neem tree extract and insect pests, herbaceous juice, Karanja tree extract, etc., insect pests (Hemiptera; Aphids), mite (Acarina; Mites), moth (Lepidoptera; Moths), worm (Hemiptera; Scale Insects, Diptera (flies) and other physiological changes and the effect of the plant natural extract control composition to find out the effects on the pathogens and pests of these plants to provide the most suitable combination ratio for pesticides and sterilization of pests It is a technical problem.

In order to achieve the above object, the present invention is a vegetable natural pest as an active ingredient a mixed composition of the extract ( Azadirachta indica ) and the herbaceous acid (pyroligneous liquid), Chrysanthemum cinerariaefolium extract as an active ingredient A control composition and a method for producing the same.

Nim (Azadirachta indica) is native to India, Nepal and Myanmar. It is an evergreen tree that grows up to 2.5m thick and about 10-20m high, and is widely distributed in South Asia, India, Africa, and Latin America. The flower has a sweet white fragrance and has 5 petals. Fruits resemble olives and are yellow with a circular green color. The fruit of the tree is from 3 to 5 years old and the most fruitful harvest can be obtained from the 10 year old tree.

Chrysanthemum cinerariaefolium is a perennial plant of the Asteraceae, originating in the Balkan Peninsula, Dalciatia. Its height reaches 60cm and stems are connected one by one, and long petioles are found in the root leaves. The leaves are deeply divided into feathers two or three times, and the torn leaf pieces are narrow. In June, long stalks bloom, and the head of the flower grows about 3cm in diameter.

Wood vinegar is liquefied liquid obtained from the process of making charcoal from wood to remove toxic and harmful substances. It is pale red in color and has a strong smell. When the wood is burned and yellowish white smoke is generated, the kiln entrance is closed, leaving only a small vent. In contact with the smoke and outside air to collect the liquid naturally cooled and falling. The collected wood vinegar is aged and separated, solubility in water is used as wood vinegar and oil solubility is used for industrial purposes. Acidic before and after pH3, constituents 80-90% water, 3% acetic acid. In addition, it contains up to 200 kinds of minerals such as formic acid, fatty acid and methanol. It is used instead of pesticides in agriculture, and in livestock industry to remove manure odors and odors or to feed livestock.

In particular, insects that can be used only by Nim alone can be used as Heliothis armigera (mythimna moth), Mythimna separate (dypium moth), Sitotroga cerealella (barley moth), Petinophora gossypiella (cotton thigh moth), Indarbela quadrinotata, Melanogromyza obtuse, Piesma quqdratum, Callosobruchus chinensis (red weevil), Nilaparvata lugens (rice weevil), Callosbruchus maculates (larvae), Pieris brassicae (rattle moth), Rabila frontalis, Apogonia blancharid bis, Multi-color Ams Phyllocnistus citrella (citrus moth), Rotylenchulus reinform (nematode), Tribolium confusum (chicken stew), Oryctes rhinoceros (helmet), Rhopaloispum maidis, Corcyra cephalonica (rice worm moth), Aphis hydrangea , Pelopidas mathias, Schistocera gregaria, Melodidogyne incognita, Plutella xylostella, Meloidogyne javanica, Plutella maculipennis, Pratylenchus genus, Orseolis oryzae, Tribo lium castaneum, Rhizopertha dominica, powdered beetle, Oryzaephilus surinamensis, Heloithis armigera ), Earias vittela (chestnut moth), Stomopteryx nertaria (peanut stalk moth), Scirpophaga incertulas (modified moth), Lymantria dispar (straw moth), Melanagromyza phaseoli (sad moth), Amsacta moorei (beech white light moth), Tylenchorhynchus brass Root-knot nematodes, Trogoderma granarium, Starch aphid, Melanaphis sacchaari, Sugarnake moth, Cnaphalocrocis medicella, Moth, Aproaerema modicella, Microtermes species, and terminus Nephotettix virescenes (Tobacco seminar), Sogatella furcifera (white light bulb), Epilachna variverstis (bean weevil), Bemisia tabaci (cigarette powder), Locusta migratoria (pulmuchi), Lipaphis erysimi There is water, etc.), Athalia lugens proxima (Eastern muip bees), Crocidomia binotalis (cabbage moth net).

The extraction method of the extract of neem is a methanol tank extraction method. After finely pulverizing the nume kernel of the nymph tree, 100g of sample is put into a circular flask, and 150ml of methanol is added to the top part of the sample. = 1: 1.5), after standing for 3 days at room temperature, the flask was placed on the heating mantle and heated to 75 ℃ to recover the evaporated methanol using a cooling apparatus and the above process was repeated three times or more to extract enough. The extracted extract (40 ml per 100 g of sample) was filtered using a filter paper, and then dried by removing the methanol using a vacuum evaporation apparatus to obtain a dried neem extract powder. Neem extract powder contains natural ingredients such as nimvidin and azadilactin, which are insecticidal and bactericidal secondary metabolites.

10 g of the obtained Nim extract powder was dissolved in 25 ml of ethanol, and 75 ml of a high-quality surfactant (Tween80) and 400 ml of water were also used.

The wood vinegar of the present invention is not intended for drinking or for use in the human body, but for improving soil and supplying trace elements, more preferably for applying insecticidal activity. For the manufacturing process, general waste wood, solid wood, etc. were used, and extracted from the carbonizer, which was allowed to stand for 6 months to 1 year, and the stratified liquid was used.

Pyrethrum extract was purchased from a cultivated farmer's farm, and the extraction method of Pyrethrum was a methanol tank extraction method. After sufficiently drying the buds of Pyrethrum, they were crushed finely, put 100g of sample into a circular flask, and the top part of the sample was slightly submerged. After adding 150 ml of methanol (Sample: Methanol = 1: 1.5), the mixture was left at room temperature for 3 days, and then the flask was placed on a heating mantle and heated to 75 ° C to recover the evaporated methanol using a cooling apparatus. Repeated more than once and extracted sufficiently. The extracted extract (40 ml per 100 g of sample) was filtered using a filter paper, and then dried by removing the methanol using a vacuum evaporator to obtain Pyrethrum extract powder. 10 g of the obtained Pyrethrum extract powder was dissolved in 25 ml of ethanol, and 75 ml of higher surfactant (Tween80) and 400 ml of water were also used.

Hereinafter, the present invention will be described in detail with reference to the following Examples.

Example 1

After finely crushing the neem kernel (Neem kernel), put 100g of sample in a circular flask, add 150ml of methanol so that the top of the sample is slightly submerged, and leave it at room temperature for 3 days, and then place the flask on the heating mantle. The methanol was evaporated by heating to 75 ℃ was recovered by using a cooling apparatus, and the above process was repeatedly extracted three times or more, and then the extracted extract liquid (40 ml per 100 g of sample) was filtered using a filter paper, followed by a reduced pressure evaporator. After the removal of methanol to prepare a dried neem extract powder, after dissolving 10 g of the prepared neem extract powder in 25 ml of ethanol, 75 ml of a higher surfactant (Tween80) and 400 ml of water were added to prepare a neem extract,

Using wood waste, solid wood, etc., the wood vinegar extracted from the carbonizer is left to stand for 6 months to 1 year to prepare the wood vinegar (stratum).

After sufficiently drying the buds of P. erythema (Chrysanthemum), pulverize, put 100g of sample in a circular flask, add 150ml of methanol so that the top of the sample is slightly submerged, and leave it at room temperature for 3 days, and then place the flask on the heating mantle. After heating up to 75 ℃ and recovering the evaporated methanol using a cooling apparatus, and repeating the above process three times or more, the extraction process was sufficiently extracted, and then the extract solution (40ml per 100g of sample) was filtered using a filter paper, followed by a vacuum evaporation apparatus. After removing methanol using to prepare the dried Pyrethrum extract powder, after dissolving the prepared Pyrethrum extract powder 10g in 25ml of ethanol and 75ml of higher surfactant (Tween80) and 400ml of water to prepare the Pyrethrum extract,

The plant extracts were prepared by mixing the nymph extract, wood vinegar solution (medium layer solution) and wormwood extract with a volume ratio of 1: 1: 1.

Examples 2-4

The production method was the same as in Example 1 except that the plant extracts, plant vinegar (medium layer) and the pesticide soup extract with different volume ratios were prepared as plant natural pest control agents as follows.

Nim: Pesticide country: Mixed grass rate aphid Mite moth Norinjae Paris Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) 100: 0: 0 50.0 100 52.2 100 46.7 100 40.0 100 30.0 100 Example 1 1: 1: 1 61.3 123 60.3 116 53.0 113 49.5 124 38.7 129 Example 2 2: 1: 1 75.6 151 71.5 137 66.7 143 67.8 170 43.9 146 Example 3 4: 1: 1 81.0 162 76.3 146 78.4 168 71.9 180 56.8 189 Example 4 9: 1: 1 86.7 173 82.2 157 86.7 186 76.7 192 73.3 244

Experimental Example 1 Breeding of Caterpillars

Insecticidal test specimens were all reared without exposure to pesticides. Plutella xylostella larvae were bred at a temperature of 27 ± 1 ℃, relative humidity of 55 ± 5%, and light conditions of 16L: 8D and used for pesticidal activity experiments. Spotted mite ( Tetranychus urticae ) was used for insecticidal activity experiments by feeding kidney beans under temperature 27 ± 1 ℃, relative humidity 55 ± 5%, light conditions 16L: 8D. Peach aphid (Myzus persicae) was used for insecticidal activity experiments by feeding tobacco at 27 ± 1 ℃, 55 ± 5% relative humidity, and 16L: 8D in light conditions. Riptortus clavatus was used for insecticidal activity experiments by raising soybeans at a temperature of 27 ± 1 ℃, relative humidity of 55 ± 5%, and light conditions of 16L: 8D. The housefly (Musca domestica) was used for insecticidal activity experiments by feeding chick feed, whole milk powder and sugar at a temperature of 27 ± 1 ℃, relative humidity of 55 ± 5%, and light conditions of 16L: 8D.

Forest pests were collected from July-August 2000 by outdoor insects such as Corythuca ciliata, Tent moth (Malacosoma neustria testacea), Agelastica coerulea, and Hyphantria cunea larvae. Was used.

In order to adapt to the indoor conditions, the experiment was used to screen only the second-third-year-old infants with excellent vitality after 3 days. Willow, teal and plane leaves were used to feed each of the caterpillars.

Experimental Example 2: Control effect on aphids and mites

The activity test of peach aphid was carried out by cutting tobacco leaf three weeks after transplantation into 7.5cm diameter disk and immersing it in the prepared solution for about 30 seconds. It was stored and tested under the conditions of ± 1 ℃, relative humidity 55 ± 5%, light conditions 16L: 8D.

The active assay of spotted mite was cut into 3 cm diameter soybean leaves about 3 weeks after sowing, cut into 9cm diameter petri-dish, and then wetted with distilled water, and placed on the prepared leaf disk. Thirty female M. larvae were inoculated in three replicates, sprayed with a small atomizer, and stored at 27 ± 1 ° C., relative humidity 55 ± 5%, and light conditions of 16L: 8D.

Experimental Example 3 Control Effect on Moths

The activity test of Chinese cabbage root moth is 4 weeks after transplanting, cut the cabbage leaf into 7.5cm diameter disk and immerse it in the prepared chemical solution for 30 seconds, dry it sufficiently, and put it into 9cm diameter Petri-dish. The animals were inoculated in three replicates and stored at 27 ± 1 ° C., relative humidity 55 ± 5%, and light conditions of 16L: 8D.

Experimental Example 4: Control effect on stink bug

The activity test of sawtooth ant henlin rinsing material is supplied to the cotton wool with about 3 weeks of sowing after sowing into a 4cm diameter and 15cm test-tube. After spraying the chemical solution and stored at a temperature of 27 ± 1 ℃, relative humidity 55 ± 5%, light conditions 16L: 8D conditions and assayed.

Experimental Example 5 Control Effects on Flies

In the activity test of housefly, 10 adults were placed in a test tube of 4cm in diameter and 15cm in length with moistened cotton wool, and then sprayed with a small nebulizer in three repetitions. Three larvae were inoculated in three repetitions of 10 larvae, and stored at 27 ± 1 ° C., relative humidity of 55 ± 5%, and light conditions of 16L: 8D.

 Nim: Herbal mixture mixture ratio aphid Mite moth Norinjae Paris Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) 100: 0 50.0 100 52.2 100 46.7 100 40.0 100 30.0 100 95: 5 60.0 120 62.2 119 60.0 128 50.0 125 33.3 111 80:20 63.3 127 62.2 119 57.0 122 53.3 133 50.0 167 50:50 53.3 107 53.3 102 53.0 113 46.7 117 46.7 156 0: 100 33.3 67 24.4 47 36.7 79 20.0 50 30.0 100

Nim: Mixed insect ratio aphid Mite moth Norinjae Paris Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) Lethality (%) Relative Efficiency (%) 100: 0 50.0 100 52.2 100 46.7 100 40.0 100 30.0 100 95: 5 76.7 153 73.3 140 70.0 150 76.7 192 43.3 144 80:20 83.3 167 76.7 147 86.7 186 70.0 175 73.3 244 50:50 63.3 127 63.3 121 53.3 114 50.0 125 56.7 189 0: 100 33.3 67 36.7 70 53.3 114 26.7 67 46.7 156

The present invention as described above is a kind of biological pesticide whose main ingredient is a natural ingredient extracted from the plant, and after a certain time after spraying, it selectively decomposes in a natural state and does not leave any residue, thus selectively acting only on pests and germs that damage the plant. It is a natural pesticide that is harmless to humans.

In addition, it protects the health of spraying farmers, disturbs ecosystems such as pests or disturbs the nervous system to control them, does not produce resistance and can be used for a long time, and enables eco-friendly farming to spread organic farming.

Claims (3)

After finely crushing the nucleus of the fruit of Nim, put 100g of sample in a circular flask, add 150ml of methanol so that the top part of the sample is slightly submerged, and leave it at room temperature for 3 days, and then place the flask on the heating mantle and place it at 75 ℃. The process of recovering the methanol evaporated by heating with a cooling apparatus was repeatedly extracted at least three times in total, and then the extracted extract (40 ml per 100 g of sample) was filtered using filter paper, and then the methanol was removed using a reduced pressure evaporator. Next, to prepare a dried neem extract powder, dissolving 10 g of the prepared neem extract powder in 25 ml of ethanol, and then preparing a neem extract by adding 75 ml of an advanced surfactant (Tween80) and 400 ml of water; Preparing the wood vinegar by leaving the wood vinegar extracted from the carbonizer for 6 months to 1 year using general waste wood or solid wood; After sufficiently drying the buds of P. erythema (Chrysanthemum), pulverize, put 100g of sample in a circular flask, add 150ml of methanol so that the top of the sample is slightly submerged, and leave it at room temperature for 3 days, and then place the flask on the heating mantle. After the extraction, the process of recovering the evaporated methanol by using a cooling device was repeatedly extracted three times or more. Then, the extract (40 ml per 100 g of sample) was filtered using a filter paper, followed by using a reduced pressure evaporator. Removing the methanol to prepare a dried Pyrethrum extract powder, dissolving the prepared Pyrethrum extract powder in 25 ml of ethanol, and then preparing a Pyrethrum extract by adding 75 ml of a higher surfactant (Tween80) and 400 ml of water; Mixing the nim extract, wood vinegar solution and Pyrethrum extract in a ratio of 1: 1: 1 to 9: 1: 1 by volume; method of producing a plant natural pest control composition comprising a. A plant-based natural pest control agent comprising a mixed composition of a nim extract, a wood vinegar solution and a pesticide extract prepared by the method of claim 1 as an active ingredient. delete