CN102498984B - Method for preventing and treating plutella xylostella by applying lemongrass essential oil - Google Patents
Method for preventing and treating plutella xylostella by applying lemongrass essential oil Download PDFInfo
- Publication number
- CN102498984B CN102498984B CN 201110289743 CN201110289743A CN102498984B CN 102498984 B CN102498984 B CN 102498984B CN 201110289743 CN201110289743 CN 201110289743 CN 201110289743 A CN201110289743 A CN 201110289743A CN 102498984 B CN102498984 B CN 102498984B
- Authority
- CN
- China
- Prior art keywords
- diamond
- essential oil
- preparation
- liquid
- plutella xylostella
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention discloses a method for preventing and treating plutella xylostella by applying lemongrass essential oil and belongs to the technical field of agricultural pest control. The method comprises the following steps of: (1) preparation of solution; (2) preparation of prevention and treatment and/or repellent liquor; (3) prevention and treatment of larvae of the plutella xylostella; (4) repellent application of oviposition of the plutella xylostella; and the like. The method has 31.7 percent to 67.3 percent of preventing and treating effect on the larvae of the plutella xylostella and has 30.4 percent to 72.7 percent of repellent effect on the oviposition of the plutella xylostella. The lemongrass essential oil is a vegetative pollution-free product. According to the invention, the varieties of medicaments for preventing and treating the plutella xylostella are increased, the harmful effect of conventional pesticides on the environment can be reduced, and the method has obvious ecological benefit and social benefit. The method can be popularized and applied in vegetable production areas.
Description
Technical field
The present invention relates to the agricultural insect pests control technical field, be specifically related to the appliable plant essential oil---the lemon grass essential oil carries out the method for integrated control to diamond-back moth.
Background technology
Diamond-back moth (Plutella xylostella L.) belongs to Lepidoptera diamond-back moth section, is a kind of insect of worldwide phytophagy, and its host reaches more than 40 kinds, the brassicaceous vegetable of mainly causing harm., since becoming the primary pest of brassicaceous vegetable, 20 century 70 diamond-back moths seriously occured such as provinces such as Guangdong, Hainan, Fujian, Yunnan, Hubei in south in China always.In recent ten years, along with the adjustment of north industry structure and the expansion of growing vegetables area, causing harm of diamond-back moth also is obvious ascendant trend.The diamond-back moth generation generation is many, the generation overlap phenomenon is serious, reproduction speed is fast, and difficulty of prevention and cure is large, often makes blade be netted, and deterioration in quality, output fall sharply even have no harvest.So depending at the very start, people use the organic synthesis insecticide.According to estimates, the expense of the annual control in whole world diamond-back moth is up to more than 1,000,000,000 dollars.Along with insecticide uses in a large number, widely, the diamond-back moth resistance occurs, the speed of development is very fast, to cause the very high insecticide of many original preventive effects also one after the other to reduce or to have lost its control efficiency, now become one of insect of the most serious and refractory reason of pesticide resistance.Simultaneously, the rise of current pollution-less agriculture has proposed Secretary to the use of agricultural chemicals, clearly ban use of severe toxicity, high poison, high residue and carcinogenic, teratogenesis, mutagenesis agricultural chemicals, and the consumption of available agricultural chemicals, working concentration, use age etc. have all been done strict control.
In the long-term evolution process of plant and insect, plant is to avoid causing harm of plant-feed insect, form or induce and produced distinctive secondary compound itself, these compounds are called as Plant Secondary Metabolites matter or plant secondary substance, and most insects is played the chemical defence effect.In recent years, relevant these Plant Secondary Materials compounds and insect are subjected to its post-stimulatory behavior performance to become the focus of scientific research.According to the chemical defence type of plant to insect, namely the Plant Secondary Materials compound can be divided into the effect of plant-feed insect lure, the effect such as repellent, poisoning and food refusal.Contain in the plant insect do not like or some disagreeable secondary compound, this is exactly their protectant concerning plant self, and defence consumer's effect is arranged.Utilize described Plant Secondary Materials compound to control diamond-back moth also and then be developed to plant protection product and obtained certain achievement: such as North America Chinese juniper and Huashan pine extract diamond-back moth is had good Antifeedant Effects and the deterrent effect of laying eggs, integrated control to diamond-back moth has preferably effect (Xu Shicai etc., 5 kinds of coniferals leaf extracts are to the research of diamond-back moth food refusal and the deterrent effect of laying eggs. the Jiangsu agricultural science, 2008,3:99～101); Five-leaved chaste tree extract and pinellia tuber extract also have the goodish deterrent effect of laying eggs to the diamond-back moth adult, and the lasting period is grown (Yuan Shikai's Tomb etc., the five-leaved chaste tree extract is to the diamondback moth larvae virulence and to the deterrent effect of laying eggs of adult. Chinese Journal of Applied Ecology, 2006,17 (4): 695～698; In lijin steps etc., pinellia tuber extract is avoided Oviposition of Diamondback Moth and is kept away and ovicidal action. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2010,35 (9): 1108～1111).
Lemon grass (Cymbopogon citrates) has another name called lemon grass (Cymbopogon citratus), lemon-grass, belongs to the grass family Cymbopogon, is herbaceos perennial.Lemon grass originates in the ground such as India, Southeast Asia, now has been distributed widely in subtropical and tropical zones, such as India, Sri Lanka, Indonesia, Thailand, Vietnam, South America and North America, Africa, Brazil, Paraguay, Britain and other places.China mainly plants the area and is Guangdong, Hainan and Taiwan etc.Lemon grass has pure and fresh lemon smell, all is widely used at seasoning, essence and flavoring agent industry and pharmaceutical sanitary field.In addition, have report lemon grass essential oil to have good effect exterminating mosquito, its Main Function composition is citral, but relevant its also do not have relevant report aspect agricultural insect pests control.
Summary of the invention
The present invention seeks to, for the rapid and existing agricultural chemicals of resistance development of the diamond-back moth problem such as residual serious in vegetables, proposes a kind of new control medicament to diamond-back moth effect uniqueness, Plant Secondary Materials compounds that environmental pollution is little; Another object of the present invention is the concrete application method that proposes to utilize this medicament control diamond-back moth in vegetables produce.
The object of the invention is achieved through the following technical solutions.
Use the method for lemon grass essential oil control diamond-back moth, the method is carried out according to the following steps:
(1) preparation of solution: cosolvent and by volume 1: 999 ratio of water are mixed with solution, for subsequent use;
(2) prevent and treat and/or avoid the preparation that keeps away liquid: with lemon grass essential oil and solution by volume 1: the 49-99 ratio is mixed with control and/or avoids and keep away liquid, and is for subsequent use;
(3) to the control of diamondback moth larvae: the liquid of step (2) preparation at crop diamond-back moth 1-3 instar larvae occurrence in peak period, is sprayed to plant by the consumption of 30-40L/ mu and uses;
(4) avoiding of Oviposition of Diamondback Moth kept away application: at crop diamond-back moth adult occurrence in peak period, even spraying is to the positive and negative of plant leaf and stem stalk, and the loss that does not produce liquid with the liquid of step (2) preparation.
Described cosolvent is choosing any one kind of them in farming breast 100, polysorbas20 or the Tween 80.
The invention has the beneficial effects as follows:
1, the present invention with the lemon grass essential oil be mixed with the control and/or avoid keep away liquid after, diamondback moth larvae is had the control efficiency of 31.7%-67.3%; Laying eggs of diamond-back moth had the rate of keeping away of avoiding of 30.4-72.7%, and preferably lasting effect is arranged, avoid the rate of keeping away after 4 days and still reach 69.1%, significantly reduced the egg laying amount of diamond-back moth on the chemicals treatment host plant, reduced harm, reached the purpose of protective plant;
2, to adopt the lemon grass essential oil be the control medicament of diamond-back moth in the present invention, both increased the medicament kind of control diamond-back moth, increased the selectivity of medication, the harmful effect that environment is caused to alleviate common insecticides; Again to further investigate from now on its active insecticidal components, environmental friendliness vegetable insecticide by direct utilization and bionical synthetic means development of new has preferably theory and practice meaning, tool is ecology and social benefit preferably;
3, lemon grass essential oils plant non-harmful product, although the present invention shows only is its material medicine control or avoids the effect of keeping away Oviposition of Diamondback Moth, but existing experimental data shows, composite its effect by synergist will be significantly improved, so this agent has preferably application prospect.
Embodiment
The present invention is described in further detail by following examples and test example, but should be appreciated that the present invention is not limited by following content.
It is 100% that embodiment and test example all adopt content, by the lemon grass essential oil product of Xuancheng city tree Hui Xiangcaoyuan production.
Embodiment 1:(prevents and treats and/or avoids the preparation that keeps away liquid and use 1)
(1) preparation of solution: cosolvent farming breast 100 and by volume 1: 999 ratio of water are mixed with solution, for subsequent use;
(2) prevent and treat and/or avoid the preparation that keeps away liquid: to be mixed with concentration be the control of 20.0uL/mL and/or avoid and keep away liquid with by volume 1: 49 ratio of lemon grass essential oil and solution, for subsequent use;
(3) to the control of diamondback moth larvae: the liquid of step (2) preparation at each 1-3 instar larvae occurrence in peak period from generation to generation of crop diamond-back moth, is sprayed to plant by the consumption of 30L/ mu and uses all over the body;
(4) avoiding of Oviposition of Diamondback Moth kept away application: at each adult occurrence in peak period from generation to generation of diamond-back moth, even spraying is to the positive and negative of plant leaf and stem stalk, and the loss that does not produce liquid with the liquid of step (2) preparation.
Embodiment 2:(prevents and treats and/or avoids the preparation that keeps away liquid and use 2)
(1) preparation of solution: cosolvent polysorbas20 and by volume 1: 999 ratio of water are mixed with solution, for subsequent use;
(2) prevent and treat and/or avoid the preparation that keeps away liquid: to be mixed with concentration be the control of 15.0uL/mL and/or avoid and keep away liquid with by volume 1: 65.7 ratio of lemon grass essential oil and solution, for subsequent use;
(3) to the control of diamondback moth larvae: the liquid of step (2) preparation at each 1-3 instar larvae occurrence in peak period from generation to generation of crop diamond-back moth, is sprayed to plant by the consumption of 35L/ mu and uses all over the body;
(4) avoiding of Oviposition of Diamondback Moth kept away application: at each adult occurrence in peak period from generation to generation of diamond-back moth, even spraying is to the positive and negative of plant leaf and stem stalk, and the loss that does not produce liquid with the liquid of step (2) preparation.
Embodiment 3:(prevents and treats and/or avoids the preparation that keeps away liquid and use 3)
(1) preparation of solution: cosolvent Tween 80 and by volume 1: 999 ratio of water are mixed with solution, for subsequent use;
(2) prevent and treat and/or avoid the preparation that keeps away liquid: to be mixed with concentration be the control of 10.0uL/mL and/or avoid and keep away liquid with by volume 1: 99 ratio of lemon grass essential oil and solution, for subsequent use;
(3) to the control of diamondback moth larvae: the liquid of step (2) preparation at each 1-3 instar larvae occurrence in peak period from generation to generation of crop diamond-back moth, is sprayed to plant by the consumption of 40L/ mu and uses all over the body;
(4) avoiding of Oviposition of Diamondback Moth kept away application: at each adult occurrence in peak period from generation to generation of diamond-back moth, even spraying is to the positive and negative of plant leaf and stem stalk, and the loss that does not produce liquid with the liquid of step (2) preparation.
Test example 1:(Toxicity Determination)
" blade infusion process " adopted in test.The lemon grass essential oil is mixed with respectively the liquid that concentration is 20.0uL/mL, 15.0uL/mL and 10.0uL/mL according to embodiment 1,2,3; Every processing is with supplying 15 of examination diamond-back moth primary larvas in 3 age, and three repetitions are established in every processing; The fresh vegetable blade is soaked 10s in above-mentioned liquid, after slightly drying, put into 10 centimeters of diameters, high 6 centimeters insect box, put into 3-4 sheet dish leaf in every box, putting into temperature is 25 ± 1 ℃ again, and relative moisture is 60-80%, and illumination/interlunation is than being raising in the climatic cabinate of 14h: 10h; Respectively at checking dead borer population behind raising 24,48, the 72h and the borer population of living, add up at last the lethality (seeing Table 1) that each concentration is processed lower diamond-back moth.
Table 1 lemon grass essential oil is to the Toxicity Determination result of diamondback moth larvae
The field controling test of test example 2:(diamond-back moth)
1 experimental condition
1.1 the selection of subjects, crop and kind: diamond-back moth (Plutclla xylostella Linnaeus), wild cabbage (ball dish)
1.2 environmental condition: Zhejiang Jiaxing, residential quarter bar shaped farming
2 experimental scheme and arrangement
2.1 medicament
2.1.1 test medicine: the lemon grass essential oil is mixed with respectively the liquid that concentration is 20.0uL/mL, 15.0uL/mL and 10.0uL/mL according to embodiment 1,2,3;
2.1.2 dosing and numbering
Each treatment agent of table 2 and consumption
| Process numbering | The medicament title | Effective component content (uL/mL) |
| 01 | No. 1, lemon grass essential oil | 20.0 |
| 02 | No. 2, lemon grass essential oil | 15.0 |
| 03 | No. 3, lemon grass essential oil | 10.0 |
| 04 | CK (newborn 100 aqueous solution of farming) | 0 |
| 05 | CK (the polysorbas20 aqueous solution) | 0 |
| 06 | CK (the Tween 80 aqueous solution) | 0 |
2.2 the residential quarter arranges
2.2.1 arrange the residential quarter: district's group is arranged at random
2.2.2 residential quarter area and repetition: residential quarter area 33m
2Number of repetition 4 times
3 application methods
3.1 using method: be watered even spraying
3.2 spraying equipment: Jacto type manual sprayer
3.3 spraying time and number of times: in dispenser on the 15th in 06 month in 2010 once, contained the phase of sending out for diamond-back moth at that time, wherein the 1-3 instar larvae accounts for 87.6%, tests and does not use any medicine control in front 14 days
4 investigation, record and measuring method
4.1 dispenser was fine the same day, 30 ℃ of maximum temperatures, 22 ℃ of minimum temperatures, 25.7 ℃ of daily mean temperatures, 26 ℃ of duration of test daily mean temperatures, duration of test co-falling rain 0.0mm; Light soil glues field, middle fertility
4.2 investigation method, time and number of times
4.2.1 control time and number of times: each investigation in 1,3,7 day is once investigated four times altogether before medicine and behind the medicine
4.2.2 investigation method: 15 strains are demarcated in every residential quarter, investigate its all insect population quantity
4.2.3 drug effect computational methods
5 results and analysis (seeing Table 3)
Table 3 control diamond-back moth field control effectiveness test result
Annotate: through 5% significance test
Test example 3:(Oviposition of Diamondback Moth avoid keep away test)
According to embodiment 1,2,3 are diluted to 20.0uL/mL with the lemon grass essential oil, 15.0uL/mL and 10.0uL/mL, select the identical potted plant green vegetables seedling of growing way, with Jacto type manual sprayer with leave dual sides and the stem stalk of dilution even spraying at potted plant little green vegetables, naturally will process green vegetables after drying and contrast green vegetables and be staggered to dependent insect cage, every cage is put into and is processed and each 2 strain green vegetables seedling of contrast, access the diamond-back moth adult in 10 couples of emergence 24h, honey cotton balls with 10% supplements the nutrients, place 25 ℃ greenhouse, respectively at 24,48,72, check the ovum amount of diamond-back moth on the cabbage heart seedling behind the 96h.
Table 4 lemon grass essential oil is to the deterrent effect of laying eggs of diamond-back moth
Effect:
In sum, use the former medicine of lemon grass essential oil diamondback moth larvae is had the control efficiency of 31.7%-67.3%, the diamond-back moth adult is had the deterrent effect of laying eggs of 30.4-72.7%, and the lasting period is longer, avoids the rate of keeping away after 4 days and still reaches 69.1%.When vegetables spray this type of essential oil; not only can reduce diamond-back moth insect population number; but repellent diamond-back moth Adult worms producting eggs also; this is to reducing diamond-back moth worm source density; reduce diamond-back moth harm and develop green vegetables production and play an important role; the resistance of having brought after having avoided simultaneously chemical agent to use, residual and rampant problem again are for from now on exploitation and the development of such novel vegetable plant protection product laid a good foundation.
Claims (1)
1. use the method for lemon grass essential oil control diamond-back moth, it is characterized in that carrying out according to the following steps:
(1) preparation of solution: cosolvent and by volume 1: 999 ratio of water are mixed with solution, for subsequent use; Wherein said cosolvent is choosing any one kind of them in farming breast 100, polysorbas20 or the Tween 80;
(2) prevent and treat and/or avoid the preparation that keeps away liquid: with lemon grass essential oil and solution by volume 1: the 49-99 ratio is mixed with control and/or avoids and keep away liquid, and is for subsequent use;
(3) to the control of diamondback moth larvae: the liquid of step (2) preparation at each 1-3 instar larvae occurrence in peak period from generation to generation of crop diamond-back moth, is sprayed to plant by the consumption of 30-40L/ mu and uses;
(4) avoiding of Oviposition of Diamondback Moth kept away application: at each adult occurrence in peak period from generation to generation of diamond-back moth, even spraying is to the positive and negative of plant leaf and stem stalk, and the loss that does not produce liquid with the liquid of step (2) preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110289743 CN102498984B (en) | 2011-09-27 | 2011-09-27 | Method for preventing and treating plutella xylostella by applying lemongrass essential oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110289743 CN102498984B (en) | 2011-09-27 | 2011-09-27 | Method for preventing and treating plutella xylostella by applying lemongrass essential oil |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102498984A CN102498984A (en) | 2012-06-20 |
| CN102498984B true CN102498984B (en) | 2013-04-24 |
Family
ID=46211166
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110289743 Expired - Fee Related CN102498984B (en) | 2011-09-27 | 2011-09-27 | Method for preventing and treating plutella xylostella by applying lemongrass essential oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102498984B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577212A (en) * | 2016-10-29 | 2017-04-26 | 蚌埠市涂山绿园蔬菜科研专业合作社 | Planting method of environment-friendly and nuisanceless broccoli |
| CN115191416A (en) * | 2022-07-05 | 2022-10-18 | 武汉轻工大学 | Method for preventing and controlling stored object pests |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101128118A (en) * | 2005-01-24 | 2008-02-20 | 博塔诺凯普有限公司 | Formulations containing microencapsulated essential oils |
| CN101569317A (en) * | 2009-06-11 | 2009-11-04 | 福建省农业科学院植物保护研究所 | Chlorpyrifos microemulsion containing chenopodium ambrosioides oil and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS579705A (en) * | 1980-06-23 | 1982-01-19 | Shin Etsu Chem Co Ltd | Sex pheromone substance releasing tube |
-
2011
- 2011-09-27 CN CN 201110289743 patent/CN102498984B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101128118A (en) * | 2005-01-24 | 2008-02-20 | 博塔诺凯普有限公司 | Formulations containing microencapsulated essential oils |
| CN101569317A (en) * | 2009-06-11 | 2009-11-04 | 福建省农业科学院植物保护研究所 | Chlorpyrifos microemulsion containing chenopodium ambrosioides oil and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 张赟彬等.香辛料精油的生物活性研究进展.《上海应用技术学院学报(自然科学版)》.2011,第11卷(第2期),85-89,94. * |
| 柠檬桉精油对小菜蛾幼虫生物活性的研究;唐为芳等;《中国园艺文摘》;20100430(第4期);175-176 * |
| 植物精油对几种害虫的毒杀活性;侯华民等;《天然产物研究与开发》;20020630;第14卷(第6期);27-30 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102498984A (en) | 2012-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103535389B (en) | Plant source fruit tree insecticide | |
| CN103636660B (en) | A kind of for preventing and treating the Environmental-protection particle agent of Sugarcane Pests | |
| CN103548895A (en) | Botanical vegetable insecticide | |
| CN105638767A (en) | Efficient plant pesticide and preparation method thereof | |
| CN105284864A (en) | Forest fruit trunk-injection insecticide and preparation method and application thereof | |
| CN101589716A (en) | The compounded pesticides of Osthole and matrine and application thereof | |
| CN108041083B (en) | Plant source pesticide synergist and application thereof | |
| CN104542685A (en) | Compound insecticide and application thereof | |
| CN103598266B (en) | Non-polluted pesticide with pure traditional Chinese medicinal components, as well as preparation method and application thereof | |
| CN103355322B (en) | Compound biological insecticide and application thereof | |
| CN105211094A (en) | Woods fruit insect protected liniment, preparation method and application | |
| CN102498984B (en) | Method for preventing and treating plutella xylostella by applying lemongrass essential oil | |
| CN102484994B (en) | Synergy composition of matrine and chlorantraniliprole | |
| CN110786344A (en) | Method for preventing and treating downy mildew of fruits and vegetables by utilizing baeckea frutescens branch and leaf extracting solution | |
| Irwan et al. | Effectiveness of Annona squamosa and Annona muricata Seed Extracts as Ingredients in Bio-pesticides Spray | |
| CN102487966B (en) | Botanical pesticide for effectively controlling Dioryctria splendidella Herrich-Schaeffer, preparation thereof and application thereof | |
| CN104855432A (en) | Complex insecticide and application thereof | |
| CN104094980A (en) | Azadirachtin water aqua for treating Chinese chive root maggot and preparation method thereof | |
| Veena et al. | Effectiveness of plant oils for increasing the efficacy of insecticides and acaricides against chilli mite | |
| Adesina et al. | Efficacy of some aqueous plant extracts against cotton stainers, Dysdercus superstitious (Herrich Schaffer)(Hemiptera: Pyrrhocoridae) | |
| CN107333819A (en) | A kind of natural matrine insecticide and preparation method thereof | |
| WO2011077588A1 (en) | Agent for elimination/control of bursaphelenchus xylophilus nikle, process for production of the agent, and method for prevention from pine wilting damage by bursaphelenchus xylophilus nikle | |
| CN103918705B (en) | Pesticidal combination containing spiral shell worm ethyl ester and gamma cyhalothrin and application thereof | |
| El-Massad et al. | Insecticidal potentiality of Datura innoxia leaf extracts against the cluster bug (Agonoscelis pubescens (Thunberg)) | |
| CN106332919A (en) | Pesticide for preventing Arbela dea and preparing method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130424 Termination date: 20170927 |