KR102328358B1 - Composition for acaricide containing cedarwood oil - Google Patents

Abstract

The present invention relates to a composition for acaricides for mites comprising cedarwood oil, and to an acaricide for mites comprising the same.
When the cedarwood oil of the present invention is included, it is eco-friendly and economical because it can provide an effective acaricidal effect against mites even with a small amount. In addition, when cedarwood oil and additional vegetable oil and emulsifier are included, it is possible to enhance the acaricidal effect of cedarwood oil, thereby providing a more effective acaricidal composition for mites.

Description

A composition for acaricide containing cedarwood oil {Composition for acaricide containing cedarwood oil}

The present invention relates to a composition for acaricides for mites comprising cedarwood oil, and to an acaricide for mites comprising the same.

The chicken mite ( Dermanyssus gallinae ) is an ectoparasite belonging to the acaridae family of the suborder mites and suborder mites, parasites on birds and sucks blood. Ticks parasitize chickens and suck blood mainly at night, and during the day they hide themselves in shaded areas or crevices, making it difficult to find them. After hatching, the young tick is about 0.5mm in length and has 3 pairs of legs. Adult ticks are 1 mm long and have 4 pairs of legs. Adult mites sometimes exceed 1 mm in the blood-sucking stage immediately after blood sucking, so they show a black-red appearance due to blood. The amount of blood sucked per bird is very small, but in large farms, chickens sometimes die from anemia.

Chicken mites become larval mites after hatching from eggs and become primary juvenile mites without blood-sucking process. The first juvenile mite becomes a second juvenile mite within 24 hours after the blood sucking, and thereafter, it further sucks blood to become a mature mite. In about 9 days from the egg, it becomes a mature tick and repeats bloodsucking and spawning.

The scale of damage caused by chicken mites in domestic poultry farms was large in the order of occurrence of contaminated eggs caused by tick excreta or blood sucking mites bursting, lowering of egg laying rate due to stress, anemia or death due to blood sucking, and immunity to vaccination due to chicken mite parasitism. Decreased response has also been reported. In addition, it is reported that the scale of damage affecting workers, such as discomfort or allergies, is considerable.

In addition, chicken mites frequently mediate various bacterial and viral infections through blood sucking. In developing countries, it has been reported that chicken mites are involved in multiple infectious diseases, such as salmonella infection and pig-only infection. Salmonella bacteria proliferated in chicken mites are transmitted to the next generation through eggs. In Japan, chickenpox outbreaks were also confirmed in chicken mite-contaminated cages, and the chickenpox virus was detected from chicken mites collected from the cages. Since chickenpox-infected chickens have a history of vaccination against chickenpox, it is predicted that there is a possibility that stress caused by chicken mite parasitism may have some effect on the immune system.

Due to the recent mechanized housing, the temperature and humidity environment inside the housing has become constant throughout the year. As a result, chicken mites proliferate regardless of the season, increasing damage to laying hens caused by chicken mite parasitism. Moreover, it is difficult to control by the appearance of chicken mites that show resistance to drugs or by exceptional drugs approved for use.

Currently, pyrethroids, carbamates, and organophosphorus are representative drugs used for controlling chicken mites in Korea. fipronil) is also used. A domestic veterinary drug company has launched a product called "Wagupuri" containing formic acid and 2-tert-butyl-5-(4-tertbutylbenzylthio)-4-chloropyridazin-3(2H)-one, but Therefore, it is called "Wagupri Blue" containing Bifenthrin(2-methyl[1,1'-biphenyl]-3-yl-methyl3-(2-chloro-3,3,3-trifluoro-1-propenyl2 and 2-dimethylcyclopropanecarboxylate). On the other hand, some laying hen farms are negatively using pesticides, which are chemical organic pesticides for agriculture, indiscriminately. Overseas, they are controlled by fumigation using plant oil ingredients, and chemical acaricides are used as in Korea. In other words, there are many cases of using chemical agents for chicken mites or replacing organic synthetic pesticides that negatively suppress pests of crops, so harmful chemicals or pesticides may remain in livestock products produced on farms. Ingestion of it may adversely affect the health of the public.

Korean Patent Application No. 10-2016-0074410 relates to a chicken mite acaricide composition using a natural plant extract, and provides an acaricide composition for chicken mites comprising neem oil and a ginkgo leaf extract. However, there is a need for further research on the combination with an emulsifier for an effective and stable acaricide formulation while containing a smaller amount of plant extract.

Accordingly, the present inventors have made an earnest effort to provide a composition for effectively killing mites, particularly chicken mites, in a more environmentally friendly and economical way in livestock farms. As a result, it was confirmed that cedarwood oil exhibits acaricidal activity against chicken mites, and when used by mixing a specific surfactant mixture with this mixture as an emulsifier, the acaricidal activity against chicken mites can be significantly enhanced and completed the present invention. did.

Accordingly, it is an object of the present invention to provide a composition for acaricides for mites comprising cedarwood oil to an acaricide for mites comprising the same.

The present invention provides a composition for acaricides for mites comprising cedarwood oil, and an acaricide for mites comprising the same.

According to a preferred embodiment of the present invention, the cedarwood oil may be at a concentration of 0.02 to 1.5% by weight.

According to a preferred embodiment of the present invention, the cedar wood oil may be included in an amount of 2 to 50% by volume.

According to a preferred embodiment of the present invention, the acaricidal composition may additionally include a vegetable oil.

According to a preferred embodiment of the present invention, the vegetable oil may be any one or more selected from the group consisting of rapeseed oil, castor oil, corn oil and edible oil.

According to a preferred embodiment of the present invention, the vegetable oil may be included in an amount of 30 to 80% by volume.

According to a preferred embodiment of the present invention, the composition for acaricide may additionally include an emulsifier.

According to a preferred embodiment of the present invention, the emulsifier is methyl oleate (Methyl oleate), 2-dodecoxyethanol (2-dodecoxyethanol), polyoxyethylene monooleate (Polyoxyethylene monooleate), Span 80 (Span 80) And it may be any one or more selected from the group consisting of Tween 80 (Tween 80).

According to a preferred embodiment of the present invention, the emulsifier may be included in an amount of 15 to 30% by volume.

According to a preferred embodiment of the present invention, the HLB (Hydrophilic-lipophilic balance) value of the emulsifier may be 7 to 15.

According to a preferred embodiment of the present invention, the mites are any one selected from the group consisting of house dust mites, hair mites, ear mites, large-legged dust mites, vertical pattern mites, stored food mites, small small mites and chicken mites. It may be more than

When the cedarwood oil of the present invention is included, it is eco-friendly and economical because it can provide an effective acaricidal effect against mites even with a small amount. In addition, when the vegetable oil and the emulsifier are additionally included in the cedarwood oil, synergy occurs to enhance the acaricidal effect of the cedarwood oil, thereby providing a more effective composition for acaricide.

1 shows the acaricidal effect of natural plant oil alone on chicken mites. It was confirmed that cedarwood oil exhibited a higher level of acaricidal effect than other natural plant oils.
Figure 2 shows the confirmation of the acaricidal effect on chicken mites of a composition for acaricide comprising cedarwood oil, corn oil and an emulsifier 80mix-11. Compared to the case of cedarwood oil alone, when corn oil and 80mix-11 of emulsifier were additionally included, it was confirmed that even a low cedarwood oil concentration showed 100% acaricidal effect.
Figure 3 shows the confirmation of the acaricidal effect of ingredients other than cedarwood oil. Sterile water alone did not show the acaricidal effect, and the emulsifier 80mix-11, 80mix-11 and corn oil, Tween 80 or Span 80 alone showed some acaricidal effect, but it was confirmed that it was only about 60%.

Hereinafter, the present invention will be described in more detail.

As described above, pesticides or acaricides previously used to suppress pests such as mites are organic synthetic chemicals, and may have a bad effect on the health of livestock or humans who consume them.

Accordingly, the composition for acaricide comprising cedarwood oil of the present invention can provide an excellent acaricide effect against eco-friendly mites by excluding organic chemicals even when including cedarwood oil alone. In addition, in the case of including an emulsifier and corn oil having an HLB value of 11 to 12 by mixing Span 80 and Tween 80 in addition to cedarwood oil, the acaricidal effect significantly increased compared to the case of including cedarwood oil alone is economical and can be provided continuously.

The 'acaricidal effect' of the present invention means the effect of controlling mites such as mites, and may be a concept included in the 'insecticide effect', which is the effect of controlling all insects or pests.

Therefore, the present invention can provide a composition for acaricides for mites comprising cedarwood oil to acaricides for mites comprising the same.

Cedarwood oil of the present invention has an acaricidal effect on mites if the concentration is 0.02% by weight or more ([Table 1], [Table 4] and [Fig. 2]). According to a preferred embodiment of the present invention, the cedarwood oil may be preferably at a concentration of 0.02 to 1.5% by weight, and more preferably at a concentration of 0.04 to 1% by weight.

According to a preferred embodiment of the present invention, the cedarwood oil may be included in an amount of 2 to 50% by volume, and more preferably, it may be included in an amount of 3 to 45% by volume.

According to a preferred embodiment of the present invention, the acaricidal composition may additionally include a vegetable oil.

According to a preferred embodiment of the present invention, the vegetable oil may be any one or more selected from the group consisting of rapeseed oil, castor oil, corn oil and edible oil, preferably corn oil.

According to a preferred embodiment of the present invention, the vegetable oil may be included in an amount of 30 to 80% by volume, and more preferably, it may be included in an amount of 35 to 75% by volume.

In order to formulate a biological material into a composition for acaricide, a certain amount of a surfactant is required as an emulsifier for stabilization of product properties and convenience in use. Since these surfactants should be harmless to the human body and eco-friendly materials, an emulsifier to be included with the cedarwood oil of the present invention was selected for safe raw materials.

According to a preferred embodiment of the present invention, the composition for acaricide may additionally include an emulsifier.

According to a preferred embodiment of the present invention, the emulsifier is methyl oleate (Methyl oleate), 2-dodecoxyethanol (2-dodecoxyethanol), polyoxyethylene monooleate (Polyoxyethylene monooleate), Span 80 (Span 80) And it may be any one or more selected from the group consisting of Tween 80 (Tween 80). Preferably, the emulsifier is methyl oleate (Methyl oleate), 2-dodecoxyethanol (2-dodecoxyethanol), polyoxyethylene monooleate (Polyoxyethylene monooleate), span 80 (Span 80) and Tween 80 (Tween 80) It may be a mixture of two types selected from the group consisting of, and more preferably, the emulsifier may be a mixture of Span 80 and Tween 80. The span 80 and tween 80 may be mixed at 10 to 70 volume % and 35 to 90 volume %, respectively, preferably 15 to 60 volume % and 40 to 85 volume %, respectively, more preferably may be mixed in an amount of 30 to 40% by volume and 60 to 70% by volume, respectively.

According to a preferred embodiment of the present invention, the emulsifier may be included in an amount of 15 to 30% by volume, and more preferably, it may be included in an amount of 20 to 25% by volume.

According to a preferred embodiment of the present invention, the HLB (Hydrophilic-lipophilic balance) value of the emulsifier may be 7 to 15. Preferably, the HLB value may be 8 to 14, and more preferably, the HLB value may be 11 to 12.

According to a preferred embodiment of the present invention, the composition for acaricide containing all of the lavender oil, emulsifier and corn oil may be diluted at a dilution ratio of 1/100 to 1/400, preferably 1/200. It can be used diluted to a dilution ratio of 1/300.

According to a preferred embodiment of the present invention, the mites are any one selected from the group consisting of house dust mites, hair mites, ear mites, large-legged dust mites, vertical pattern mites, stored food mites, small small mites and chicken mites. It may be more than Preferably, it may be a chicken mite ( Dermanyssus gallinae ).

The composition for acaricide or the acaricide comprising the same may be used by any one or more methods selected from the group consisting of a contact method, a fumigation method, an immersion method and a leaf immersion method, and may preferably be used as a contact method. The contact method may be a method of treating the composition for acaricide on filter paper and attaching it to the bottom of a Petri dish and then inoculating the mites therein or spraying the composition directly onto the pests with a sprayer. The fumigation method may be a method of inoculating mites after treating the acaricidal composition on a paper disk and attaching it to the microtube lid and then inoculating the mites there, or treating the composition on filter paper and attaching it to the Petri dish lid. The immersion method may be a method of immersing mites in the composition for acaricide. The leaf immersion method may be a method of inoculating the mites after treating the acaricidal composition on the leaves of the host plant.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it is obvious to those of ordinary skill in the art that the scope of the present invention is not to be interpreted as being limited by these examples.

Confirmation of the acaricidal effect of cedarwood oil

Cedarwood ( Cedrus atlantica , Bubble bank) oil was to determine whether it can exhibit acaricidal effect on chicken mites ( Dermanyssus gallinae ).

Specifically, a large amount of chicken mites were collected from a laying hen farm, and the population was maintained at a temperature of 25° C., a relative humidity of 60%, and a dark condition until the experiment. In a plant culture plate having a width of 63.5 cm ² , a culture plate was prepared by puncturing the plate lid so that the mites could breathe and closing with a fine mesh, and an average of 500 animals were separated from each plate. After that, the cedarwood oil was diluted to 0.8%, 0.4%, 0.2%, 0.1%, 0.05% or 0.025% of the surfactant Tween 80 with a final concentration of 10%, and applied to each plate using a sprayer. 2 ml each was uniformly sprayed in an amount of 31.5 μl/cm ^{2 .} Twenty-four hours after spraying, immobile individuals were considered dead mites. Rosemary ( Rosmarinus officinalis , Bubble bank) oil, basil ( Ocimum basilicum , Bubble bank) oil, sweet orange ( Citrus sinensis , Bubble bank) oil, eucalyptus ( Eucalyptus globulus , Bubble bank) oil, sunflower ( Helianthus annuus , FLORA) oil, Peppermint ( Mentha Piperita , Bubble bank) oil and mustard ( Brassica nigra , Pure indian foods) oil was used as a control, and sterile water was sprayed as a negative control. All salvi tests were repeated three times.

As a result, as shown in [Table 1] and [Fig. 1] below, it was confirmed that cedarwood oil had the best acaricidal effect on chicken mites.

control Flesh ratio (%) negative control 0 plant oil dilution concentration Average flesh ratio (%) and
Number of individuals (salvi/total) plant oil dilution concentration Average flesh ratio (%) and population (flesh/survival) Cedarwood 0.8% 100 (1617/1617) sunflower 0.8% 0 (0/1433) 0.4% 100 (1441/1441) 0.4% 0 (0/1425) 0.2% 84.07 (1432/1705) 0.2% 0 (0/1596) 0.1% 9.30 (151/1586) 0.1% 0 (0/1608) 0.05% 6.73 (97/1371) 0.05% 0 (0/1449) 0.025% 0 (0/1716) 0.025% 0 (0/1624) Rosemary 0.8% 95.16 (1374/1445) sweet
Orange 0.8% 81.06 (1227/1514) 0.4% 8.79 (135/1571) 0.4% 29.77 (458/1539) 0.2% 0 (0/1535) 0.2% 0 (0/1392) 0.1% 0 (0/1499) 0.1% 0 (0/1470) 0.05% 0 (0/1636) 0.05% 0 (0/1547) 0.025% 0 (0/1466) 0.025% 0 (0(1323) eucalyptus
Tooth 0.8% 55.08 (836/1535) basil 0.8% 78.25 (1152/1456) 0.4% 47.69 (727/1536) 0.4% 75.08 (1153/1518) 0.2% 11.31 (168/1489) 0.2% 51.24 (778/1586) 0.1% 0 (0/1534) 0.1% 22.44 (361/1558) 0.05% 0 (0/1652) 0.05% 0 (0/1378) 0.025% 0 (0/1470) 0.025% 0 (0/1484) mustard 0.8% 0 (0/1582) peppermint 0.8% 0 (0/1505) 0.4% 0 (0/1630) 0.4% 0 (0/1512) 0.2% 0 (0/1466) 0.2% 0 (0/1562) 0.1% 0 (0/1383) 0.1% 0 (0/1576) 0.05% 0 (0/1603) 0.05% 0 (0/1485) 0.025% 0 (0/1597) 0.025% 0 (0/1606)

Selection of optimal conditions for surfactant composition for emulsifiers

<2-1> Emulsification test

It was attempted to select an emulsifier to be included in formulating the cedarwood oil selected in <Example 1> into a composition for acaricide.

Specifically, Tween 80 and Span 80, surfactants corresponding to EPA lists 4A and 4B, were used as candidate substances. Two types of surfactants were formulated according to HLB (hydrophilic-lipophilic balance) values of 9, 11.25, or 13.1, respectively, 80mix-9 (HLB 9), 80mix-11 (HLB 11.25), 80mix-13 ( HLB 13.1). Then, cedarwood 40%, corn oil 37% and emulsifier 80mix-9, 11 or 13 were added at 23% each and mixed for 24 hours. After 24 hours, emulsification was visually observed and judged by dividing it into four levels: excellent (+++), good (++), average (+), and poor (-).

As a result, as shown in [Table 2] below, it was confirmed that the emulsification properties of all oil layers were excellent.

division HLB Span 80(%) Tween 80 (%) 80mix-9 9 56 44 80mix-11 11.25 35 65 80mix-13 13.1 18 82 division Ingredient name Addition ratio (%) Formulation results oil layer 1 Cedarwood Oil 40 +++ corn oil 37 80mix-9 23 oil layer 2 Cedarwood Oil 40 +++ corn oil 37 80mix-11 23 oil layer 3 Cedarwood Oil 40 +++ corn oil 37 80mix-13 23

<2-2> Verification of persistence of emulsified state

While using the oil-soluble layer containing cedarwood oil as an acaricide composition, in order to check the persistence of the emulsified state, each of the oil layers 1, 2 or 3 prepared in Example <2-1> is diluted 1/250 in sterilized water, respectively. The durability was evaluated by comparison.

Specifically, 4 ml of oil layer 1, 2 or 3 was added to 996 ml of sterile water and the persistence of the emulsified state was observed at room temperature for 20 days. At the time of observation, it was judged by dividing it into four levels: excellent (+++), good (++), average (+), and poor (-).

As a result, as shown in [Table 3] below, the oil layer 2 showed the best durability, and finally, the oil layer 2 was selected from the three types of formulations.

division Elapsed time (days) Persistence Results oil layer 1 5 +++ 10 +++ 15 ++ 20 ++ oil layer 2 5 +++ 10 +++ 15 +++ 20 +++ oil layer 3 5 +++ 10 ++ 15 ++ 20 ++

Confirmation of the acaricidal effect of the acaricidal composition

<3-1> Confirmation of acaricidal effect of acaricide composition comprising cedarwood oil and emulsifier

An acaricide composition comprising cedarwood oil selected in <Example 1> and <Example 2> and an emulsifier 80mix-11 was prepared.

division Ingredient name Addition ratio (V/V, %) Final concentration (%) floor detailed ingredients floor detailed ingredients detailed ingredients Formulation 1
Cedarwood 40 oil layer Cedarwood Oil 0.4 40 0.16 80mix-11 23 0.092 corn oil 37 0.148 water layer sterile water 99.6 100 99.6 Formulation 2
Cedarwood 30 oil layer Cedarwood Oil 0.4 30 0.12 80mix-11 23 0.092 corn oil 47 0.188 water layer sterile water 99.6 100 99.6 Formulation 3
Cedarwood 20 oil layer Cedarwood Oil 0.4 20 0.08 80mix-11 23 0.092 corn oil 57 0.228 water layer sterile water 99.6 100 99.6 Formulation 4
Cedarwood 10 oil layer Cedarwood Oil 0.4 10 0.04 80mix-11 23 0.092 corn oil 67 0.268 water layer sterile water 99.6 100 99.6 Formulation 5
Cedarwood 5 oil layer Cedarwood Oil 0.4 5 0.02 80mix-11 23 0.092 corn oil 72 0.288 water layer sterile water 99.6 100 99.6

Specifically, as in the composition of [Table 4], 5, 10, 20, 30 or 40% of cedarwood oil, 23% of emulsifier 80mix-11 and 37, 47, 57, 67 or 72% of corn oil as a whole After adjusting 100%, it was put in a mixer and mixed for about 10 minutes to prepare an oil layer. 4 ml of the prepared oil layer was added to 996 ml of sterile water, and emulsified for 5 minutes to prepare a prototype. The acaricidal effect was performed in the same manner as in <Example 1>.

As a result, as shown in [Table 5] and [Fig. 2], the acaricidal effect on chicken mites in Formulations 1 to 4 containing cedarwood oil was 100%, and in Formulation 5, cedarwood oil alone It was confirmed that it exhibited a significantly superior acaricidal effect than the case where it was included.

division Number of individuals (salvi/total) average weight ratio and
Number of individuals (salvi/total) round 1 round 2 round 3 Formulation 1
Cedarwood 40 528/528 423/423 488/488 100 (1677/1677) Formulation 2
Cedarwood 30 581/581 464/464 442/442 100 (1661/1661) Formulation 3
Cedarwood 20 512/512 527/527 503/503 100 (1467/1467) Formulation 4
Cedarwood 10 448/448 473/473 536/536 100 (1583/1583) Formulation 5
Cedarwood 5 238/415 388/581 361/515 64.74 (987/1511)

<3-2> Confirmation of the acaricidal effect of cedarwood oil and other ingredients

When cedarwood oil was treated alone as in <Example 1>, the flesh ratio did not reach 100% at 0.2% or less, whereas cedarwood oil was 0.16% as in Example <3-1> Even when it was less than or equal to, the percentage of flesh was 100%. In order to understand the cause, it was attempted to confirm the acaricidal effect of other ingredients other than cedarwood oil included in the acaricide composition.

Specifically, as shown in Table 6 below, each component included in the acaricide composition was mixed with the same amount included in the acaricide composition, and then diluted with a dilution ratio of 1/250 and treated. The acaricidal effect was performed in the same manner as in <Example 1>.

As a result, as shown in [Table 6] and [Figure 3], it was confirmed that 80mix-11 used as an emulsifier, surfactant Tween 80 or Span 80 had an acaricidal effect on chicken mites.

As a result, it was determined that the cedarwood oil of the present invention can exhibit an excellent acaricidal effect together with an emulsifier compared to the case of using alone. That is, if the acaricide composition is diluted 1/250 and applied, it is eco-friendly and economical because it is possible to control chicken mites with natural plant oil ingredients while excluding organic chemicals.

division Ingredient name Addition ratio (V/V, %) Final concentration (%) Average flesh ratio and number of individuals (flesh/total) floor detailed ingredients floor detailed ingredients detailed ingredients ingredient 1 water layer sterile water 100 100 100 0 (0/1681) ingredient 2 oil layer 80mix-11 0.4 23 0.092 59.10 (902/1516) sterile water 77 0.308 water layer sterile water 99.6 100 99.6 ingredient 3 oil layer 80mix-11 0.4 23 0.092 60.20 (951/1578) corn oil 77 0.308 water layer sterile water 99.6 100 99.6 ingredient 4 oil layer Tween 80 0.4 15 0.06 19.78 (324/1636) sterile water 85 0.34 water layer sterile water 99.6 100 99.6 ingredient 5 oil layer Span 80 0.4 8 0.032 52.05 (842/1614) sterile water 92 0.368 water layer sterile water 99.6 100 99.6

Claims (12)

A composition for acaricides comprising cedarwood oil, and additionally comprising a vegetable oil and an emulsifier,
The emulsifier is a mixture of Span 80 (Span 80) and Tween 80 (Tween 80), the composition for acaricide mites, characterized in that contained in an amount of 20 to 25% by volume.
According to claim 1, wherein the cedarwood oil is a composition for acaricide mites, characterized in that the concentration of 0.02 to 1.5% by weight.
According to claim 1, wherein the cedarwood oil is a mite acaricide composition, characterized in that it comprises 2 to 50% by volume.
delete According to claim 1, wherein the vegetable oil is rapeseed oil, castor oil, corn oil and edible oil, characterized in that any one or more selected from the group consisting of mites acaricide composition.
According to claim 1, wherein the vegetable oil is a mite acaricidal composition, characterized in that it comprises 30 to 80% by volume.
delete delete delete According to claim 1, wherein the emulsifier HLB (Hydrophilic-lipophilic balance) value is 7 to 15, characterized in that the acaricidal composition.
The method according to claim 1, wherein the mites are at least one selected from the group consisting of house dust mites, hair mites, ear mites, large-legged dust mites, vertical pattern mites, stored food mites, small small mites and chicken mites. A composition for killing mites.
An acaricide for mites comprising the composition for acaricide of claim 1.

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