KR101953984B1 - Compositions comprising Bistrifluron and Indoxacarb having Insecticidal Activities - Google Patents

Abstract

The present invention relates to a composition for controlling insects, which comprises bistriprolactone and phosphoruscabe as an active ingredient. The composition is excellent in quick-acting and delayed action against pests, and can be particularly useful for complete eradication of cockroach populations .

Description

[0001] Compositions comprising Bistrifluron and Indoxacarb having Insecticidal Activities [

The present invention relates to a composition for pest control comprising bistriprolauron and phosphoruscabe as effective ingredients.

Pests cause various damages to property such as human body, crops, livestock and houses. Pests include insect pests, hygiene pests, food pests, stored pests, property pests, livestock pests, and agricultural pests, among which the cockroaches are hygienic pests that carry germs, viruses, molds, Accordingly, 40 kinds of diseases such as food poisoning, atopy and asthma can be induced.

Cockroaches are found all over the world, and there are inhabitants of East, East, and Germany wheels. The German wheel in the dining room produces three to four algae in a lifetime, and an algae contains about 40 to 50 eggs. The females of the German wheel bear an algae at the end of their abdomen. The eggs hatch about 20 days after laying eggs, and the born birds become adult after about two months. The cockroach females are known to save sperm for a lifetime with a single mating, and to continue laying eggs without the male. Thus, since the scattering frequency of the cockroach is high and the number of eggs scattered is large, the majority of the cockroaches can be regenerated from the remaining adult or egg unless the cockroach is completely eradicated.

In the case of commercially available cockroach-control products, there are many constituents consisting of one active ingredient, and mainly have a rapid-acting property. Recently, studies on insecticides for controlling cockroaches by mixing active ingredient drugs having different characteristics have been conducted. For example, a medicament containing a mixture of prophetampose and hydroprene (IGR system), a medicament containing a mixture of hydra methylenone and propolis, a medicament containing a mixture of hyosyphylose and boric acid, and hydramethyluron or chlorpyrifos These drugs are fast acting. When adults and nymphs are ingested, they can damage the epidermis and cause a lasting effect. However, since the female wheel is killed too quickly or the drug efficacy does not spread to the inner ear, In many cases.

Accordingly, the inventors of the present invention have made intensive researches for discovering a composition for controlling a new pest, and as a result, the present inventors have completed the present invention by confirming that a composition comprising bistriprolactone and phosphoruscabe is excellent in quick- .

Korean Patent Publication No. 10-2004-7012723

It is an object of the present invention to provide a pest control composition comprising bistrifluoro and phosphoruscarb as an active ingredient.

Another object of the present invention is to provide a method for controlling pests comprising the step of disposing a composition for controlling pests comprising bistriprolauron and phosphoruscabe as an effective ingredient in such a manner that the composition can contact the insect pests at a place where pests appear .

An aspect of the present invention provides a composition for controlling insects, which comprises bistriprolauron and phosphoruscabe as an active ingredient.

Phosphorus oxycarbine is an oxadiazine-based insecticide effective ingredient, which is a fast-acting drug that works by blocking the sodium channel in the nervous system of insects.

In addition, bistripylrone is a drug of insect growth regulators (IGR) system, inhibits chitin biosynthesis and inhibits peeling, and is active mainly for controlling moth larvae.

As used herein, the term " control " means preventing or remediating, directly or indirectly, the alleviation or elimination of the harmful insects causing damage to humans, As shown in FIG.

According to one embodiment of the invention, the composition comprises 1 to 4% by weight of bistrifluoro; And 0.1 to 0.6% by weight of phosphoruscabe.

According to one embodiment of the present invention, the insect pest may be at least one member selected from the group consisting of termites, wrinkle ants, mosquito larvae, cockroach eggs, cockroach nymphs and cockroach adults. In particular, Not only adults, but also cockroaches and cockroach parasites have a protective effect. That is, since it functions throughout the life cycle of the cockroach, the cockroach community can be effectively eradicated.

According to one embodiment of the present invention, the bistrifluron can inhibit the formation of algae of the female cockroach.

Alopecia suppression means that the female cockroach does not form a normal alopecia after mating, and the alopecia includes an alopecia suppressed when compared with a normal alopecia.

According to one embodiment of the present invention, the composition may have a cockroach sequestrant activity.

As used herein, the term " sequenced insect " means that a pest that has not been exposed to the pesticide is contacted with the pest exposed to the pest control agent, The cockroach which has not fed the composition of the present invention feeds on the food containing the composition, the other body of the cockroach or the body or the carcass which occurs after eating the composition, by the other cockroach, As the active ingredient of the present invention is delivered from the cockroach to the cockroach, the cockroach which does not directly feed the medicament has an effect of controlling the drug.

According to one embodiment of the present invention, the composition may further comprise a pheromone attracting cockroach or a prey that cockroaches prefer. The incentive of cockroaches means to attract cockroaches regardless of the distinction of adult / nymphs or male / female cockroaches.

The pheromone used to attract the cockroach may be an aggregate pheromone or a sex pheromone, for example, the sex pheromone may be ferriplaon B ((1Z, 5E) -1,10 (14) -diepoxy- 9-one), Blattellaquinone (Science, 18 February 2005: Vol. 307, No. 5172, 1104-1106), cis-9-tricycene, The set pheromone can be, for example, but is not limited to, 1- (N, N-dimethylamino) -2-methyl-2-propanol, trimethylamine and dimethylamine.

Meanwhile, the composition of the present invention may include a prey that the cockroach likes to attract cockroaches. For example, it may be cereal powder, legume powder, animal powder, saccharide, attractant, water or any mixture thereof, or may be any of those conventionally used for inducing cockroaches or modified ones. The grain powder may be crushed corn, rice, barley, wheat or the like, chaff, rice bran, wheat bran or sake forage by refining grains, and the pulverized soybean powder may be pulverized beans, red beans or peanuts, Animal powder may be pupa powder, dried squid powder, fish meal, anchovy powder, bonito sprouts, dried yeast extract, milk powder, skim milk, skimmed milk powder, cheese or meat extract. Examples of the saccharides include saccharides such as glucose, fructose or galactose, disaccharides such as maltose, maltose or sucrose, polysaccharides such as glycogen, cellulose or starch, and attractants such as butter, chocolate, onion, paste peanut, dried yeast extract, Fish meal or anchovy powder. Fermented milk can be added to food. Fermented milk is fermented by lactic acid bacteria or yeast using milk or other milk as a raw material, or processed by fermented milk, yogurt, fermented buttermilk, acidophilus milk, kefir, It can be Kumis. In addition, there are many different kinds of cumin, cumin, anise, nutmeg, fennel, laurel, cardamom, mebouki, mexican sage (oregano), red pepper Paprika extract, dried product and / or essential oil of at least one perfume plant may be added to the food.

According to one embodiment of the present invention, the composition may be any one form selected from the group consisting of a liquid material, a paste, a powder, a granule, a bulk, and a tablet, It is the paste water that can be used. On the other hand, the composition of the present invention may contain various bases and / or additives necessary for formulation of the formulation.

Another aspect of the present invention provides a method of controlling pests comprising the step of disposing a composition for controlling pests comprising bistriprolauron and phosphoruscabe as an effective ingredient in such a manner that the composition can contact the pest at a place where the pest appears do.

The composition of the present invention can be placed directly on the site where the insect pests appear, or can be applied and placed, or can be placed using a food medium such as impregnation agent such as paper, resin and nonwoven fabric, resinous container or trapper.

According to one embodiment of the invention, the contact may be a feeding.

The cockroach has unique characteristics of feeding on the other cockroach or dead cockroach, so that the effect is transferred to another cockroach which has eaten the dead cockroach carcass or the soil by the composition of the present invention, so that the cockroach can be controlled to be sequestered.

According to the composition for insect control comprising bistriprolactone and phosphoruscabe of the present invention as an effective ingredient, it is excellent in quick-acting and delayed action against pests, and can be particularly useful for complete eradication of cockroach community populations.

Fig. 1 is a diagram showing life history of a German wheel.
FIG. 2 is a view showing a state where a German wheel is raised.
Fig. 3 is a graph showing the control of bistrifluoron contents of 0.5 and 1.0% in the German wheelworm.
Fig. 4 is a graph showing the control of the 2.0 and 4.0% content of bistripylon against the German wheelworm.
FIG. 5 is a view showing the algae of German wheel females according to whether or not the beast reflux treatment is applied.
FIG. 6 is a graph showing the number of mosquitoes hatching and the rate of hatching hatching in the hatchlings of German wheel females according to the treatment amount of bistriplullon.
Fig. 7 is a graph showing the inhibition of German wheel female adult and the rate of algae formation according to the treatment amount of bistriprolauron and imidacloprid.
FIG. 8 is a graph showing the inhibition of German wheel female adult and the rate of algae formation according to the treatment amounts of bistriprolauron and phosphoruscabe.
FIG. 9 is a picture showing a German wheel nymph that dies without being able to steadily evolve according to the treatment of the mixed nutrient of Bistrifluoron and Rhodoxacus.
FIG. 10 is a graph showing the control of Bistrifluoron and Rhodoxacus mixed dock for the wheel cluster.
Fig. 11 is a diagram showing the state of poisoning agent after 6 days of the treatment with the bistrifluoron and rhodopsacve mixed dock.
FIG. 12 is a graph showing the control of German wheel nymph according to the treatment of dead cadaver after feeding with mixed dietary dosing of bistrifluoron and rhodopsacve.

Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments, and the scope of the present invention is not limited to these embodiments.

Example  One. Bistrifluoron  And Poison serpent mixture  selection

<1-1> Cockroach breeding

The cockroach was housed in an insect feeding room at 27 ± 0.2 ° C and 50 ± 10% relative humidity. The nymphs were housed in separate breasts, and the adults were housed in the same breasts so that the female and male mated (Fig. 2).

<1-2> Beast of Reflux  Applicable to moths Other applicable insect and insecticidal activity confirmation

Applicable insect pests other than moth larvae were screened for bist reflux, which is mainly used for controlling moth larvae. As a result, insecticidal activities were found in termites, wrinkle ants, cockroach and mosquito larvae (Table 1).

Target pest Concentration (ppm) Insecticidal activity (%) Remarks termite 5000 100 Processing date 28 Wrinkle ant 5000 100 Processing date of 25 Cockroach 10000 100 Processing date 18 Mosquito larva 0.2 100 Processing Day 3

<1-3> Bistrifluoron  Determination of the effect of roach on the content

In Example 1-2, the insecticidal activity of bistripylon against cockroach was confirmed, and the effect of bistrifluoron on the cockroach was confirmed by specific contents.

Specifically, for the three-week-old German horsetail, bistriproplon was treated with a dose of 0.5, 1, 2 and 4%, respectively. The imidacloprid was treated with 2.15 %), And 10 replicates of 10 dogs per cage were used.

For the German wheel female adult dogs that produced algae, bistripluron was treated in the form of a poisonous diet containing 2 and 4% of each, while the reference drug (STD) was treated with poison containing imidacloprid in an amount of 2.15% Feeding agent Max Posegel IC (Bayer) was used and tested in 10 replicates per 10 cages.

As a result, the efficacy of bistripylon against the cockroach was increased continuously after days after treatment (DAT), especially when the content was more than 1% (FIGS. 3 and 4). In the case of cockroach females, the insecticidal effect against adult insect was lower than that of the control (Table 2), but the bistrifluoron-treated individuals did not form normal algae when compared with the control animals 5), thus showing a good hatching inhibition rate and a decrease in the number of hatching nematodes in the bistriprolactone treatment (Fig. 6).

Insecticidal effect on female adult (%) 3DAT 6DAT 10DAT 17DAT 24DAT Beast reflux (2.0%) 0.0 0.0 0.0 0.0 4.8 Beast Flourron (4.0%) 0.0 0.0 0.0 0.0 13.3 Imidacloprid (2.15%) (STD) 11.1 15.2 55.1 84.4 98.8

<1-4> Bistrifluoron  Confirmation of drug efficacy by contents of mixture

In Example 1-3, the effect of bistripylon on inhibition of the growth of roach parasitoids and inhibition of incubation of cockroach allergens was confirmed. Therefore, in order to complement the effects of short-acting and female adult, we screened partner drugs that can be used in combination with bistripruron.

Specifically, [bistriproplon + imidacloprid] was treated in the form of a poisonous diet containing [1% + 0.5%], [1% + 1%] and [1% + 1.5% (1% + 0.15%) and [1% + 0.3%], respectively, in the form of poisonous food. As a reference drug (STD), Max Poogel IC (Bayer), a poisonous food containing imidacloprid in a content of 2.15%, and Cleavate (National Healthcare), a poisonous food containing 0.6% of phosphoruscabe were used. As a test pest, female adults who did not form an algae at one week after mating were used, and 10 replicates of 10 birds per cage were tested. After 3, 6, 13, 17, 20, and 27 days after the treatment with imidacloprid, the raw water was examined and the raw water was examined after 3, 7 and 17 days after the treatment with phosphoruscavudokine. The non-treatment area examined the rate of live individuals, the ratio of live individuals to the initial population.

As a result, when compared with the imidacloprid mixture (Table 3), the insecticidal mixture (Table 4) showed an excellent algae inhibitory effect, and the insecticidal effect and fast-acting effect on female adult were excellent (Figs. 7 and 8) . In addition, the insecticidal efficiency and the inhibition of allostosis formation of the poisonous food containing 0.3% of phosphoruscabe were excellent in bistripullum and phosphoruscabe. Therefore, the final drug was selected as a drug to be used as a mixture with bistriproloxin, and the content of bistriprolactone and phosphoruscabe in the mixture was 1% and 0.3%, respectively.

Treatment agent Insecticidal effect (%) Collecting
Formation rate (%) Remarks 3DAT 6DAT 13DAT 20DAT 27DAT One No treatment 100.0 100.0 100.0 100.0 100.0 74.0 [Life Ratio] 2 Beost reflux (1%) + imidacloprid (0.5%) 0.0 3.0 38.0 65.0 99.0 17.0 Female adult
(Pre-formation) 3 Bistrifluoron (1%) + imidacloprid (1.0%) 2.0 3.0 65.0 83.0 99.0 15.0 4 Bistrifluoron (1%) + imidacloprid (1.5%) 5.0 11.0 82.0 88.0 100.0 11.0 5 Imidacloprid (2.15%) (STD) 7.0 7.0 63.0 83.0 93.0 35.0

Treatment agent Insecticidal effect (%) Collecting
Formation rate (%) Remarks 3DAT 7DAT 17DAT One No treatment 99.0 99.0 99.0 35.0 [Life Ratio] 2 Beast rifuron (1%) + phosphorus carv (0.15%) 24.2 82.8 97.0 2.0 Female adult
(Pre-formation) 3 Beast reflux (1%) + phosphorus carb (0.3%) 36.4 91.9 100.0 0.0 4 (0.6%) (STD) 82.8 99.0 100.0 0.0 5 Imidacloprid (2.15%) (STD) 7.1 12.1 71.7 12.0

Example  2. Bistrifluoron  And Poison serpent  mix Poisonous  cockroach Crowd dog Identification of body insecticidal effect

<2-1> Cockroach To the cluster object  Confirmation of drug efficacy

The insecticidal effect on the cockroach community populations of the bistrifluoron and indoxacarcomb mixture selected according to Example 1 was confirmed.

Specifically, [bistriproplon + phosphoruscab] was treated in the form of a poisonous food containing [1% + 0.3%] and as a control drug (STD), poisonous food containing 0.6% Jane Kleinbait (National Healthcare Pharmaceuticals) was used and further treated with the Blank food sample in the above-mentioned dock feed (Table 5). As a test pest, German cockroaches, female adults and male adults were used and 200 birds per cage (1.2 × 1.2 × 0.3m) (98 birds, 49 female and 53 male adults) And after 1, 4, 6, and 15 days of drug treatment, the raw water was examined. The non-treatment area examined the rate of live individuals, the ratio of live individuals to the initial population.

Treatment agent Type of food Delivery (g) Remarks One No treatment Blank 3.0 2 Beast reflux (1%) + phosphorus carb (0.3%) Dock 3.0 - 3 (0.6%) (STD) Dock 3.0 - 4 Beast Flux (1%) + Phosphorus Carb (0.3%) + Blank Feed Dock feeding + Blank 3.0 + 3.0 Comparative test when feeding conditions (integrated) 5 (0.6%) (STD) + Blank feeding Dock feeding + Blank 3.0 + 3.0

As a result, the nematode treated with bistriprolactone and rhodopsaccharide mixed docker died without being able to normally escape (FIG. 9), and the control activity against the cockroach community was excellent at 93.5% in 6DAT and 100.0% in 15DAT (Fig. 10).

<2-2> Poisonous Ingestion  Confirm

It was confirmed whether the cockroach avoids the poisonous food supplied in Example 2-1.

Specifically, the weight of the remaining poisoning agent in 6 DAT was measured relative to the initial weight of the poisonous food supplied in Table 3 of Example 2-1.

As a result, there was no significant difference in feeding rate compared to Blank food (Fig. 11), even when mixed with Blank reflux and phosphoruscab combination now with Blank poison feeding agent, about twice as much as the control drug (STD) It was confirmed that the cockroach did not evade the mixed dietary dosing of bistriprolactone and phosphoruscab (Table 6).

Treatment agent Type of food Supplied amount
(g) 6DAT Eating Rate (g) Remarks One No treatment Blank 3.0 1.78 [Life Ratio] 2 Beast reflux (1%) + phosphorus carb (0.3%) Poisonous food 3.0 1.21 - 3 (0.6%) (STD) Poisonous food 3.0 1.50 - 4 Beast Flux (1%) + Phosphorus Carb (0.3%) + Blank Feed Dock feed + Blank 3.0 + 3.0 1.63 + 1.57 Comparative test when feeding conditions 5 (0.6%) (STD) + Blank feeding Dock feed + Blank 3.0 + 3.0 0.81 + 2.04

Example  3. Bistrifluoron  And Poison serpent  mix Poisonous  Identification of the sequential insecticidal activity of roaches

The effect of sequestering insecticidal activity against cockroaches of the bistriprolactone and rhodopsinavir mixed according to Example 1 was confirmed.

Specifically, individuals who died from eating a poisonous dietary supplement containing [bistripruron + phosphoruscab] in an amount of [1% + 0.3%], and 0.6% of phosphoruscab in the control drug (STD) (Kokbo Pharmaceutical Co., Ltd.) and used dead individuals. The test pests were tested with 10 repetitions of 10 rats per cage, 4, 7, 13, and 21 days after treatment with German cockroach (2 to 3 instar), female adult , And 30 days later, respectively. The non-treatment area examined the rate of live individuals, the ratio of live individuals to the initial population.

As a result, it was found that cockroach nymphs (Table 7 and FIG. 12) that ingested dead bodies by the bistrifluorone and rhodopsin mixed dodecagonist (Table 7 and Figure 12) The insecticidal activity was high in adult (Table 8) and male adult (Table 9). Especially, in the case of ladybug cockroach, insecticidal activity was demonstrated by the effect of bistripluron. The reason for the high insecticidal activity in the dead cockroach eating the animal killed by the mixed poison feeding agent is that it takes the dead animal by the mixed poison feeding agent less risky and feeds the dead body more.

Treatment agent Insecticidal activity (%) Remarks 4DAT 7DAT 13DAT 21DAT 30DAT One No treatment 97.0 96.0 95.0 95.0 90.0 [Life Ratio] 2 Beast reflux (1%) + phosphorus carb (0.3%) 1.0 11.5 20.0 37.9 74.4 - 3 (0.6%) (STD) 0.0 1.0 0.0 13.7 34.4 -

Treatment agent Insecticidal activity (%) Remarks 4DAT 7DAT 13DAT 21DAT 30DAT One No treatment 100.0 100.0 100.0 100.0 90.0 [Life Ratio] 2 Beast reflux (1%) + phosphorus carb (0.3%) 3.0 10.0 13.0 23.0 31.1 - 3 (0.6%) (STD) 0.0 3.0 4.0 5.0 6.7 -

Treatment agent Insecticidal activity (%) Remarks 4DAT 7DAT 13DAT 21DAT 30DAT One No treatment 100.0 99.0 99.0 97.0 94.0 [Life Ratio] 2 Beast reflux (1%) + phosphorus carb (0.3%) 5.0 8.1 26.3 45.4 77.7 - 3 (0.6%) (STD) 4.0 4.0 6.1 12.4 38.3 -

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (9)

1. A composition for insect control comprising bistrifluron and indoxacarb as an active ingredient,
Wherein the pest is a cockroach. The composition of claim 1, wherein the composition comprises
1 to 4% by weight bistrifluron; And
0.15 to 0.6% by weight of indoxacarb,
By weight of the composition. delete The insect control composition according to claim 1, wherein the bistrifluron inhibits the formation of algae of a female cockroach. The insect control composition according to claim 1, wherein the composition has a cockroach sequestration activity. The insect control composition according to claim 1, wherein the composition further comprises a pheromone attracting cockroach or a prey that the cockroach likes. The insect control composition according to claim 1, wherein the composition is any one selected from the group consisting of a liquid material, a paste water, a granular material, a granular material, a bulk material and a tablet. A method for controlling a cockroach, comprising the step of placing the composition of claim 1 in a location where the cockroach can feed, at a location where cockroaches appear. delete

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