KR101298419B1 - Method and composition for controlling insect pest - Google Patents

Abstract

PURPOSE: A method for controlling insects is provided to control insects by removing all the insects. CONSTITUTION: A method for controlling insects comprises a step of installing a composition prepared by mixing smokybrown cockroach densovirus, pheromone for alluring cockroaches, and feed on a path or a place where cockroaches live. The weight ratio of the smokybrown cockroach densovirus is 0.01-1 weight%. The pheromone is periplanone B and/or cis-9-tricosene and the weight ratio thereof is 0.2-20 weight%.

Description

Pest control method and composition for pest control {METHOD AND COMPOSITION FOR CONTROLLING INSECT PEST}

The present invention relates to a pest control method and a composition for pest control.

Pests cause a variety of damage to property such as human bodies, crops, livestock and houses. Pests include unpleasant pests (spiders, ants, millipedes, etc.), sanitary pests (fly, tsetse flies, moth flies, dongae, centipedes, cockroaches, etc.), food pests (cockroaches, flies, weevils, etc.), and pests. (Weevils, corn weevils, etc.), property pests (termites, shellfish, worms, moths, moths, ants, worms, stink bugs, moths), livestock pests (backs, etc.), agricultural pests (locusts) , Fruit flies, grasshoppers, stink bugs, aphids, pods, aphids, leafworms, etc.).

For example, cockroaches are inhabited all over the world, and mainly in East Asia, food wheels, German wheels, and foreign wheels are reproduced. German wheels that reproduce in restaurants produce three to four eggs in a lifetime, and about one to one egg contains about 40 to 50 eggs. Females of German laps lay eggs in their abdomen, and their eggs hatch about 20 days after they lay. Born larvae become adults after about two months. Food wheels reproduced in a normal home lay about 20 eggs in a lifetime, and about 20 to 30 eggs are contained in one egg. To become an adult from an egg, it takes two to three years, and the adult lives about 200 days. Females of alien wheels lay eggs in the abdomen, and the eggs are placed in the abdomen for about 3 to 7 days and then attached to the shade. One egg nest contains about 6 to 18 eggs and hatches at about 40 days. In order to become an adult from an egg, it takes 5 to 9 months, and the adult lives about 200 to 700 days. In addition, the female of the cockroach is said to store sperm for a lifetime in one mating and continue to lay eggs without males. In this way, the spawning frequency and the number of spawning of cockroaches are extremely large, so that if the cockroach is not completely extinguished, the majority of cockroaches are immediately regenerated from the remaining adults or eggs.

Therefore, extermination of pests has been studied. For example, as an exterminator of pests, a dispersant of an insecticide such as organic phosphorus agent, pyrethroid agent, carbamate-based insecticide, and poison A food agent (bait agent) is used. This poison feed agent is attracting attention recently, and is a poison feed prepared by mixing pesticidal active ingredients and scavenging substances, and is installed in a passage through which pests are enjoyed, and the pest is eaten to control pests. However, the insect pests are not necessarily good, so the pest control effect is not sufficient, and since the pests such as cockroaches cannot be completely exterminated, pests such as cockroaches breed again after a certain period of time.

A pest control agent using Periplaneta fuliginosa densovirus (PfDNV) is described in Non-Patent Document 1. A cockroach rescue test on PfDNV-containing foods (containing PfDNV at 60,000 virus particles / g) prepared by mixing PfDNV virus with rat food was carried out, and the results of the tests are shown in Tables 4, 5 and Abstrat. It is described. Despite extremely high concentrations of 60,000 virus particles / g through PfDNV, the killing of cockroaches is very slow, or only 95.6% after 28 days. Therefore, with this technique, it is thought that the majority of cockroaches are formed again from the remaining cockroach or its eggs.

Biological Control 46 (2008) 94-100

Insects, such as cockroaches, cannot be completely exterminated by conventional chemical remedies. In addition, even if the method using the PfDNV virus of the nonpatent literature 1, even after 28 days, 100% cockroaches cannot be controlled. Then, an object of the present invention is to provide a method for completely extinguishing a pest and a composition to be used so that pests such as cockroaches remain even a little and do not reproduce again.

The present inventors have diligently studied a method for almost completely exterminating pests such as cockroaches, and a composition used therein. It has been found that within 10 days can completely kill the cockroach adults and eggs, and completed the present invention.

The present invention is as follows.

[1] Insects produced by mixing viruses having the ability to kill pests, pheromones that attract pests, and foods desired by pests in places where pests reproduce or in passages through which pests pass; Remedy Method.

[2] The pest control method according to [1], which controls the eggs of the pest.

[3] The pest control method according to [1] or [2], wherein the virus having the ability to kill pests is Mumbaidensovirus (PfDNV) or a modified product thereof, and the pest is a cockroach.

[4] A pest control composition comprising a mixture of a virus having the ability to kill pests, a pheromone that attracts pests, and a food that the pest prefers.

[5] The composition for controlling pests according to [4], for controlling eggs of pests.

[6] The composition for exterminating insect pests according to [4] or [5], wherein the virus having the ability to kill pests is Mumbaidensovirus (PfDNV) or a modified product thereof, and the pest is a cockroach.

According to the present invention, a method and composition for completely controlling pests such as cockroaches are provided.

1 is a photograph of a test box [A] only for a breeding box without a shielding material used in a pest control test.
2 is a photograph of a test box [B] in which a living environment is used, which was used for a pest control test.
Figure 3 is a photograph of a toroidal container by uniformly thinly coating 2 g of the PfDNV virus-containing composition over the entire bottom.
4 is a photograph showing an experimental landscape of Test Example 1 using a test box [B].
5 is a photograph showing an experimental landscape of Test Example 2. FIG.
6 is a photograph showing an experimental landscape of Test Example 3. FIG.
7 is a photograph showing an experimental landscape of a German wheel in Comparative Test Example 1. FIG.
8 is a photograph showing an experimental landscape of heterogeneous wheels in Comparative Test Example 1. FIG.

Although the above-mentioned thing is mentioned as a "pest", Insect is preferable among them. More preferably, unpleasant pests and sanitary pests are mentioned, Especially preferably, cockroaches are mentioned.

As a "virus which has the ability to kill pests", the thing with high killing effect can be selected for each pest targeted for control. For example, viruses, such as the Densovirus subfamily and the Baculovirus family, are mentioned. Examples of the densovirus subfamily virus include, for example, Muck quiddenovirus (PfDNV: derived host / pig wheel), silkworm denivirus (derived host / silkworm), honeycomb moth densovirus (Galleria mellonella). densovirus: Derived host / beehive moth), American southern peacock butterfly (Junonia coenia densovirus: Derived host / American southern peacock butterfly (Junonia coenia), Dengue aegypti densovirus (Derived host / dengue mosquito), White streak Forest mosquitoes (Aedes albopictus densovirus: derived host / white line forest mosquitoes) and the like and modified variants thereof. As the virus of the baculovirus family, for example, Nuclear polyhedrosis virus (derived host / human eye, double eye tree, maximus eye) And the like, granule disease virus (Granulosis virus: derived host / human eye), and modified substances thereof. When the pest is a cockroach, preferred viruses include PfDNV (Non-Patent Document 1, Arch Virol (1994) 138: 365-372, Arch Virol (1999) 144: 2111-2124, Acta Virol, 2000 Dec; 44 (6)). : 315-322) and its modified product (Arch Virol (2007) 152: 383-394), and the like. Moreover, in implementing the pest control method and the composition for pest control of this invention, it is preferable to use the virus which does not have an influence on a human. It is said that the virus of the densovirus subfamily generally has little effect on humans, and in particular, PfDNV has no effect on humans at all.

As a "pheromone which attracts pests," a pheromone aggregate, a sex pheromone, etc. are mentioned, for example. The pheromone does not include an alarm pheromone, a dispersed pheromone, or the like. For example, with regard to cockroaches, as a sex pheromone to attract males, periplanone B ((1Z, 5E) -1,10 (14) -diepoxy-4 (15), 5-germacadiene -9-on), blatellaquinone (Science, 18 February 2005: Vol. 307, No. 5172, 1104-1106), cis-9-tricosene, and the like, and as the aggregated pheromone, 1- (N, N-dimethylamino) -2-methyl-2-propanol, trimethylamine, dimethylamine, etc. are mentioned, Preferably, periplanone B, cis-9- tricosene, etc. are mentioned. In addition, the aggregated pheromone of the brown wing stingray (Plautia stali) includes (2E, 4E, 6Z) -deca-2,4,6-trienomethyl, and the aggregated pheromone of the oily green stink bug (Glaucias subpunctatus) (2Z, 4E, 6Z) -deca-2,4,6-trienoic acid methyl is mentioned. As the sex pheromone of Anomala rufocuprea, methyl (Z) -5-tetradecacetate is mentioned, and as the sex pheromone of Pseudococcus cryptus, 3-isopropenyl-2,2-dimethyl-1 -(3-isopenteninoyl) oxymethyl-cyclobutane, and hexapheric acid (E) -2-hexenyl and octyl butane are mentioned as a sex pheromone of a red spotted stink bug Norinster (Stenotus rubrovittatus).

Many traps or foods using the pheromone are sold. For example, Lasioderma serricorne, Plodia interpunctella, Brown-flying moth (Ephestia elutella), Rice moth (Corcyra cephalonica), False rice squirrel (Tribolium castaneum), Squirrel (Tribolium confusum) ), Powdery beetle (Rhizopertha dominica), weevil (Sitophilus zeamais), Scourge (Oryzaephilus surinamensis), ginseng beetle (Stegobium paniceum), Anthrenus verbasci, beetle (Popillia japonica) Anomala rufocuprea, Anomala cupera, Anomala schoenfeldti Ohaus, Anomala octiescostata, Exomala orientalis, Ectinohoplia obducta, Light yellow beetle diver (Phylloper) , Anomala osakana, Anomala albopilosa, White spotted radish (Protaetia orientalis). The pesticide control composition of this invention can also be prepared by mixing the marketed thing with the virus etc. which have the ability to kill pests.

As the "food which a pest prefers", the food which the pest inquires more strongly can be selected according to the pest of control object. As the food, a known or improved one can be used. For example, as a component of the food which a cockroach prefers, cereal powder, legume powder, animal powder, sugar, attracting component, and water are mentioned, These arbitrary components can be mixed and used. As grain classification, the thing which grind | pulverized maize, rice, a wheat, wheat, etc., the rice hull which by-produces at the time of grain refining, rice bran, bran, and sake lees are mentioned. Examples of the legume powder include those obtained by grinding beans, red beans, peanuts, and the like. As animal powder, pupa powder, dried squid powder, fish powder, anchovy powder, bonito powder, dry yeast extract, powdered milk, skim milk, skim milk powder, powdered cheese, meat extract, etc. are mentioned. Examples of the sugars include monosaccharides such as glucose, fructose and galactose, maltose, maltose and sucrose, and disaccharides such as glycogen, cellulose and starch. Examples of attractant components include butter, chocolate, onions, pasty peanuts, dry yeast extract, fish meal, and anchovy powder.

It is also preferable to add fermented milk to the food (Japanese Patent Laid-Open No. 10-81602). As fermented milk, what is obtained by fermenting with lactic acid bacteria or yeast using milk or other milk as a raw material, the drink processed into it, or a main raw material, for example, a lactic acid bacterium drink is mentioned. Specifically, yoghurt, fermented butter milk, ashidophyll milk, kefir, cumis, etc. are mentioned, for example. To prey, roots (cumin), galniza (anis), nutmeg (nicks), fennel (fenel), laurel (laurel), white nut (cardamom), mebouki (basil), Mexican sage (oregano), red pepper And dried products, essential oils, extracts or ole oranges of at least one aromatic plant selected from the group consisting of paprika (Japanese Patent Laid-Open No. 9-169611).

The "pest control composition" of the present invention is prepared from the above-described "viruses having the ability to kill pests", "pheromones that attract pests" and "prey which pests prefer". The composition and composition ratio of the "virus having the ability to kill pests", "the pheromone which attracts pests" and "the food which a pest prefers" are suitably changed so that the control effect may be the maximum for every pest and virus used. It is preferable.

For example, when the pest is a cockroach, the weight ratio to the composition of PfDNV, which is a "virus having the ability to kill pests" is, for example, 0.01 to 1% by weight (equivalent to 300 to 30,000 virus particles / g), Preferably it is 0.05-0.8 weight% (equivalent to 1,500-24,000 virus particle / g), More preferably, it is 0.1-0.5 weight% (equivalent to 3,000-15,000 virus particle / g). The weight ratio with respect to the composition of "the pheromone which attracts pests" is 0.2-20 weight%, Preferably it is 0.5-10 weight%, More preferably, it is 1-5 weight%. The weight ratio with respect to the composition of "feed which a pest prefers" is 50-99.7 weight%, Preferably it is 60-99.5 weight%, More preferably, it is 80-99 weight%, and the rest in a composition It can also be used as a part.

In addition, if necessary, a paste, a binder, a thickener, a gelling agent, a surfactant, a pH adjuster, a flavourant, and the like may be added to the composition as required. Vaseline is mentioned as a paste agent. Examples of the binder include water-soluble polymers such as polyvinyl alcohol, polyethylene glycol, polypropylene glycol, and polyvinylpyrrolidone. The water-soluble polymer may also be used as a thickener. Examples of the gelling agent include agar, gelatin, potato starch, corn starch, soybean meal, carrageenan, pectin, xanthan rubber, guar rubber and gellan rubber. Examples of the flavoring agent include sodium dihydroacetate.

The pest control composition of the present invention can take various forms such as liquid, paste, powder, granule, bulk, tablet and the like. Particularly preferred formulations include pastes. For example, a paste can be prepared by adding each component mentioned above to a required amount, adding water as needed, and mixing until it becomes uniform.

The pest control method of the present invention can be carried out by providing, for example, at a place where a pest grows or a passage where a pest passes.

The pest control method and the composition for controlling pests of the present invention are used for exterminating pests, and are suitable for extinguishing cockroaches, bed bugs, ants, termites, and the like, particularly cockroaches. Especially suitable cockroaches include Periplaneta fuliginosa, Periplaneta japonica, Periplaneta americana, Periplaneta japanna, Neostylopyga Rhombifolia, Kingbarna concinna, Brown wheels (Periplaneta brunnea) and the like. When exterminating cockroaches, virus infection spreads to the entire cockroach in the colony by sucking the dung of the infected cockroach in the colony. In addition, there is a characteristic of cockroaches that feed on dead cockroaches, and the virus propagating to dead cockroaches is more widely spread. In addition, as shown in Test Example 2 and Test Example 3, the pest control method and the composition for controlling pests of the present invention also have a control effect on eggs of cockroaches. In this way, by completely killing the adult cockroach and eggs of the cockroach, it is possible to completely suppress the cockroach reproduction of the next generation.

[Example]

Hereinafter, the present invention will be described in more detail with reference to Examples.

Example 1 Preparation of 0.2 wt% PfDNV Virus-Containing Composition

Cockroach sign food was prepared by adding water, such as corn, chocolate, and butter, to mix. 0.2 parts by weight of PfDNV virus (manufactured by Wuhan WDLZ Biological Technology Co., Ltd.), 1.5 parts by weight of periplanone B (manufactured by Wuhan WDLZ Biological Technology Co., Ltd.), cis-9-tree 0.5 parts by weight of Kosen (manufactured by Infinity Stage Green Biotechnology Co., Ltd.) and 97.8 parts by weight of the cockroach symbol food were mixed to prepare a 0.2% by weight (corresponding to 6,000 virus particles / g) PfDNV virus-containing composition.

Example 2 Preparation of 0.5 wt% PfDNV Virus-Containing Composition

A 0.5% by weight (corresponding to 15,000 virus particles / g) PfDNV virus-containing composition was prepared in the same manner as in Example 1 except that 0.5 parts by weight of PfDNV virus was used.

Test Example 1 Pest Control Test

Target Pests

As the target pests, three kinds of adults were used: German wheels, black wheels, and foreign wheels.

[Test Methods]

Six acrylic cases dedicated to breeding 120 cm wide x 60 cm deep and 45 cm high were prepared, and three of them were made into a test box [A] (refer to Fig. 1) only for the breeding box, and the remaining three were It prepared as test box [B] (refer FIG. 2) which prepared the living environment, such as a cockroach hiding place.

2 g of the PfDNV virus-containing composition prepared in Example 1 or 2 was evenly spread on the entire bottom of the annular container having a diameter of 1 cm x 2 mm (see Fig. 3), and the annular container was opened. Put it in a test box. One of three species of cockroaches were put into 100 or 200 animals in each test box, and reared for 20 to 112 days, and the number of survivors was examined. The test landscape is as shown in FIG.

Table 1 shows the results of the test using the 0.2% by weight PfDNV virus containing composition obtained in Example 1.

[Table 1]

Table 2 shows the results of the test using the 0.5% by weight PfDNV virus containing composition obtained in Example 2.

[Table 2]

[Test result]

In some of the 1st day, 2nd day, and 3rd day, the survival number was not confirmed. In the test using the composition of Example 1, the cockroach survival and egg hatching were not confirmed between the 11th and 112th days. In addition, in the test using the composition of Example 2, cockroach survival and egg hatching were not confirmed between the 11th and 20th days.

In the test using any of the compositions of Examples 1 and 2, the cockroaches were killed quickly. In the case of test box [A], relief of all the objects was completed within seven days. In addition, in case of test box [B], relief of all the objects was completed within 10 days. Once PfDNV enters the body of a cockroach, it is thought that death occurred in three days. The number of days for complete relief in test box [B] is due to the fact that corrugated cardboard, which is part of the equipped living environment, can be fed. However, when chemical remedies are used, complete relief is difficult. The effect of the composition of the present invention is extremely remarkable.

Moreover, at 0.2 weight% and 0.5 weight% of virus content, there was almost no difference in the speed of relief. That is, since the virus grows in the body of the cockroach, it can be seen that if the content of the food is 0.2% by weight, it is sufficient for the relief effect. In addition, this 0.2 weight% (equivalent to 6,000 virus particle / g) corresponds to the density | concentration of 1/10 of 60,000 virus particle / g used by the nonpatent literature 1. In Non-Patent Document 1, the effect of cockroach control was insufficient even when a high concentration of virus was contained. However, even when a virus having a concentration of 1/10 of the concentration was used, the combination of virus, pheromone, and prey was completely used. It was extremely remarkable that it could be saved.

If the number of cockroaches is 200, the effect is faster than the 100 tests. As a reason, since there is much quantity of the dung etc. containing a virus, it is inferred that the indirect eating of this dung etc. was added to the direct eating of the composition of this invention further.

Test Example 2 Test of Eggs on German Wheels

The females of the German wheels, unlike other cockroaches, keep their eggs in their stomachs until they hatch. The effect of the PfDNV virus-containing composition of the present invention on the eggs concentrated in the German wheel in that state was tested.

Target Pests

As the target pests, 20 German wheels of the females that were sifting were used.

[Test Methods]

An acryl-only acrylic case of 31 cm wide x 21 cm deep x 21 cm high was prepared. 2 g of the PfDNV virus-containing composition prepared in Example 1 was added to the annular container having a diameter of 1 cm x 2 mm, and the entire bottom surface of the annular container was evenly thinned. The annular container was put into the above-mentioned test box, 20 round germanic wheels were put, it was raised for 40 days, and the survival water was examined. The test landscape is as shown in FIG. In addition, the results of the test are shown in Table 3.

[Table 3]

[Test result]

Adults of the German wheel were wiped out in 3-5 days. The eggs of the German wheel usually hatched after about 20 days, but observation was continued until 40 days, but the eggs did not hatch.

In addition, when the chemical rescue agent mentioned later is used, when an adult of a German wheel dies and an egg is dropped, an adult dies, with the egg containing an egg cage with the PfDNV virus containing composition of this invention.

In the German wheel, the hatching rate decreases when the eggs are separated from the adult. Therefore, it is thought that there is a path between the adult and the egg. It is estimated that the PfDNV virus penetrates into the egg cage from the adult and completely kills the egg through the route because the adult eggs are contained after the virus is infected and died.

Test Example 3 Test for Eggs of Alien Wheels

[Test Methods]

Thirty eggs of the alien wheels which had fallen during the test of Test Example 1 were collected, and the observation was continued. The test landscape is as shown in FIG.

[Test result]

Observation was continued for two months, but did not hatch at all.

When the eggs lay down, it is assumed that the virus migrated from the infected females reproduced in the eggs and killed them.

Comparative Test Example 1 using a chemical pest control tests gujeje

[Composition for pest control containing chemical control agent]

2 parts by weight of hydramethylnon and 98 parts by weight of the cockroach symbol food described in Example 1 were mixed to prepare a composition for controlling pests.

Target Pests

As the pests of interest, adults of German wheels and hetero wheels were used.

[Test Methods]

Two breeding acrylic cases of 32 cm in width x 22 cm in depth x 21 cm in height were prepared. The entire bottom surface of the annular container having a diameter of 1 cm x 2 mm was spread evenly and evenly spread over 2 g of the composition for controlling pests including the chemical control agent, and the annular container was placed in the test box described above.

In one test box, 50 German wheels (including 15 females bearing eggs) were placed and the progress was observed. The test landscape is as shown in FIG. In the other test box, 50 alien wheels were put and the progress was observed. The test landscape is as shown in FIG.

The test results are shown in Table 4.

[Table 4]

[Test result]

As for cockroach adults, both cockroaches began to die from day 5 and continued until day 10, after which surviving individuals appeared. This surviving individual had no trace of eating up the pest control composition. It is estimated that death after the 10th day is not caused by the composition for exterminating insects, but by life.

As for the eggs of the German wheels, 15 eggs were collected at the start of the test, and 4 new eggs were produced during the test. From the 14th day to the 15th day, two eggs were hatched, and about 60 larvae were formed, and three eggs were hatched before and after 22 days, and about 90 larvae were produced. In addition, unlike Test Example 2, in this test using a chemical remedy, eggs are laid down and dropped when the inbred female dies.

As for the eggs of the alien wheels, four eggs and 14 homeless eggs were malformed during the test. The homeless egg disappears the next day and is presumed to have been eaten by an adult. On day 42 one of the four eggs hatched. In addition, new egg shells were created afterwards.

As mentioned above, about 20% of adult insects survived without eating, and it was thought that the control effect of an egg was also limited as a composition for pest control containing a chemical control agent. Therefore, after relief by the said composition was carried out once, it was thought that a colony was newly produced again.

Comparative Test Example 2 Pest Control Test Using Chemical Control

[Composition for pest control containing chemical control agent]

In the pest control composition of Comparative Test Example 1, an experiment was carried out in the same manner as in Comparative Test Example 1, using a composition for controlling pests containing the same amount of fipronil instead of hydrmethylmethyl.

As a result, the expression of the effect was about two days earlier than in Comparative Test Example 1, and the progress was almost the same.

According to the present invention, a method and a composition for completely controlling pests such as cockroaches and eggs thereof are provided.

Claims (6)

As a method for extinguishing cockroaches by installing a composition comprising a mixture of Mfquinquinovirus (PfDNV), a pheromone that attracts cockroaches, and a food that the cockroach prefers, the place where the cockroach reproduces or the passage where the cockroach passes,
The weight ratio of the mubaquine densovirus to the composition is 0.01 to 1% by weight,
The pheromone attracting the cockroach is periplanone B and / or cis-9-tricosene, and the weight ratio of the pheromone attracting the cockroach to the composition is 0.2 to 20% by weight. The method of claim 1,
Cockroach control method characterized in that to exterminate the cockroach eggs. A composition for cockroach extermination, comprising a mixture of Mfquinquinovirus (PfDNV), a pheromone that attracts cockroaches, and a food preferred by a cockroach,
The weight ratio of the mubaquine densovirus to the composition is 0.01 to 1% by weight,
The pheromones attracting the cockroaches are periplanone B and / or cis-9-tricosene, and the weight ratio of the pheromones attracting the cockroaches to the composition is 0.2 to 20% by weight. . The method of claim 3,
Cockroach control composition, characterized in that for extruding eggs of cockroach. delete delete

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