CN1469710A - Plant acaricidal composition and method for using the same composition - Google Patents

Abstract

The present invention relates to acaricides. More particularly, the present invention relates to botanical acaricides. In particular, the present invention relates to compositions and methods for controlling plant infesting acari with plant extracts and notably with compositions comprising oil extracts from Chenopodiacae and more particlarly Chenopodium ambrosioides. The invention further relates to compositions comprising such extracts as acaricidal compositions displaying a potent effect on Tetranychidae (mites) and providing the advantages of minimal development of resistance thereto, minimal toxicity to mammals, minimal residual activity and environmental compatibility. In a further embodiment, the compositions of the present invention further display insecticidal activity on plant infesting insects. In an especially preferred embodiment, the plant acaricidal composition comprises agr; terpinene, p cymene, limonene, ascaridole, iso ascaridole, thymol and carvacrol.

Description

The method of plant acaricidal composition and application said composition

Technical field

The present invention relates to miticide.More specifically, the present invention relates to botanical acaricide.Particularly, the present invention prevents and treats the compoistion and method of use of mite class with plant extracts.The invention still further relates to the botanical acaricide that has insecticidal activity in addition.

Background of invention

The mite class of feeding plant belongs to the most voracious phytophagy insect of infringement crop.Up to the present, still use synthetic pesticide or miticide to prevent and treat these insects; Yet along with each new product puts goods on the market, insect has produced resistance to these products.Although very complicated heredity and Biochemical processes are followed in the generation of resistance, the reason that synthesis of chemicals is produced resistance rapidly is because their active component only depends on one or more molecules of the same type usually.Therefore, thus organism is physiological by producing, behavior or the defense mechanism of form suppresses molecular activity contratoxin react (Roush and MacKenzie, 1987).

The spider mite usually is to use the problem that occurs behind the insecticide control insect, because many synthetic pesticides stimulate the breeding of mite class.For example, the mite analogy that contacts carbaryl, parathion-methyl or Rogor in the laboratory fast manyfold of reproduction speed (Flint 1990) of population that is untreated.In fact, spider mite as well known to those skilled in the art be the extremely difficult control of agricultural chemicals and make many agricultural chemicals increase the weight of spread (USP5,839,224) of insect owing to injured the natural prey organisms of spider mite.

At present T.urticae Koch accumulated a great deal of to all main types acaricidal invest resistant gene; Under many critical especially situations, nearly allly before can provide the agricultural chemicals of effective control all to use up (Georghiou 1990).

The latest edition of Farm Chemical Handbook (Meister, 1999) has been listed altogether 2050 kinds of miticides and insecticide, and only 48 kinds of products (2.4%) are that miticide and 69 kinds (3.4%) are thought and both can also can be used as insecticide as miticide.In addition, the most commercial miticide has the guideline that toxicity and the most of insect comprehensive administration projects of incompatibility are formulated to mammal.The open report of seldom relevant plant pesticide acaricidal activity, only openly neem extract is to 2 spider mites, and promptly the recruitment evaluation of Tetranychus urticae is tested (Sundaram et al, 1995; Mansour etal.1986) and thyme linaloe oil and thymol (Mansour et al.1986, see before) and neem extract (E1 Gangaihi et al.1996) to spider mite natural enemy, the effect of planting mite and Cheiracanthium (Chiracanthium mildei) is caught by Chile.

Existing about 270 kinds of Chenopodium plants (they belong to Chenopodiaceae, Caryophyllales (specific item is planted by=central authorities), Section Ambrina plant) with extensive hereditary variability.3 important kinds in North America are elder brother's promise lamb's-quarters (C.quinoa), lamb's-quarters and chenopodium ambrosiodies.All 3 all is cultivar (Quarles 1992).Elder brother's promise lamb's-quarters has very long cultivation history with Mexico in South America, has introduced Britain and North America at present as experimental food grain food crop (Risi and Galwey 1984).

Although the lamb's-quarters extensive growth of Chenopodium is distributed in American continent, it originates in Europe and Asia.They contain very a spot of essential oil and do not have chenopodium ambrosiodies and tangible smell that other Chenopodium plant is had.Elder brother's promise lamb's-quarters originates in South America, and is grown in the mountain area (Quarles1992) of South America usually.Its lobate grain is used for soup, stewes system food or as congee and have " Earthy Taste " just.

Not only lamb's-quarters and elder brother's promise lamb's-quarters contain seldom essential oil or volatile oil, and other composition of these plants also has the purposes of some potential control of insect.The unsaturated fatty acid of seed oil can be used as fungicide and nematocide (Malik et al.1985).Flavonoids in the lamb's-quarters has the xenogenesis depression effect and may have Fungicidally active, and the while sesquiterpene can be used as anti-feedant and steroid has effect (Qasem and Hill, 1989 that influence the larve of certain kinds of insect growth; Tumock 1985).Saponarin in elder brother's promise lamb's-quarters seed can be used for extremely mollusk or is applied to pharmacy that (Bumouf-Radosevich 1984,1985; Chandel and Rastogi 1980).

Chenopodium ambrosiodies originates from Central America, has been distributed widely in many countries at present.This plant is applied to anthelmintic agent (control intestinal parasite medicine) for many years traditionally in Latin America.Twentieth century early stage it be to be used for the treatment of one of acarid in people, cat, dog, horse and the pig body and main anthelmintic medicine of hookworm.Because there is the facility that extracts this oil from plant in this place in Baltimore, so the Chenopodium vegetable oil also is called " Baltimore oil " massive specializedly.Nineteen forties, the Chenopodium plant extracts substituted by other more effective synthetic anthelmintic.In addition, the vegetable oil wood preservative that also can be used as ship is grown surely to protect it to avoid barnacle.

Up to the present, reported that the Chenopodium plant has insecticidal activity to some insect, do not had acaricidal activity but also disclose the Chenopodium plant extracts.Yet, reported that other plant component has acaricidal activity.

United States Patent (USP) 4,933,371 disclose by the saponarin that extracts in each plant species (being yucca, saponin tree (qullaja), american agave, tobacco and Radix Glycyrrhizae) as miticide.In addition, United States Patent (USP) 4, the linalool that extracts in 933,371 vegetable oil that also disclose by each plant species such as Ceylon cinnamon, yellow camphor tree, flores aurantii, bergamot, Artemisia balchanorum wormwood artemisia, Cananga odorata, rose camphor tree and other oil extract are as miticide.Yet there are many shortcomings in these methods: increased cost thereby need extract active substance from plant because of them.And these compounds usually can not satisfy the required toxic level of mite extremely.

Seldom openly the full extract of plant has acaricidal activity.At present to have the situation of acaricidal activity only be to be disclosed in United States Patent (USP) 5,352 to disclosed whole plant extract, the neem extract in 672, and the expression neem seed extracts has acaricidal activity in this patent.Neem only is grown under the tropical climate condition.Therefore more difficulty and cost height of the commercialization of national azadirachta on the Northern Hemisphere.And, because azadirachta is a tree, before this tree can be used for preparing extract, needs to stop over and grew in several years.In addition, consider and wherein contain a large amount of variety classes compounds that the composition of Neem extract trends towards more complicated.Determine to derive from the work difficulty more of the toxicity of the extract of tree extract such as neem extract, because every kind of compound must be measured respectively.

Therefore, still need to provide a kind of new and product that can effectively kill mite that kills the problems referred to above of mite product known in the art that overcomes.Also need to provide a kind of littler miticide composition that may produce resistance that has in addition to it.Particularly, exist a kind of demand that the miticide composition that can prevent and treat various insects in the different phase of plant growing is provided.Also exist a kind of demand promptly provide use to animal particularly mammal do not have the method for the on-site mite class of composition for preventing and controlling of toxicity.

The present invention attempts to solve above-mentioned and other demand.

The lot of documents of mentioning in the present specification, the full content that is incorporated herein above-mentioned document as a reference.

Summary of the invention

Therefore an object of the present invention is to provide a kind of improved miticide that overcomes the prior art shortcoming.More specifically, the present invention relates to plant acaricide.

Provide in one embodiment of the invention and contained ascaridole; 1, the 4-peroxide is to the miticide composition of  alkene.

In one embodiment of the invention, provide a kind of composition that contains as acaricidal American wormseed oil extract.In addition, also provide and contained the American wormseed oil extract as acaricidal preparation.Also provide derive from chenopodium ambrosiodies contain terpene miticide composition (or extract).

In another embodiment of the present invention, thus also provide a kind of select suitable chenopodium ambrosiodies kind and the vegetation period method obtain to produce the acaricidal activity oil of optimal yield or the Chenopodium crop yield of the acaricidal activity promoter of optimal yield.In another embodiment of the present invention, thus also provide select suitable chenopodium ambrosiodies kind and the vegetation period method obtain to produce optimal yield and cause the reactive compound of acaricidal activity and/or the Chenopodium plant products of various active compound.In the preferred embodiment of the invention, thus provide select chenopodium ambrosiodies and the vegetation period method obtain to produce the Chenopodium plant products that optimal yield causes acaricidal activity reactive compound and/or various active compound.

In another embodiment of the present invention, the reactive compound in the miticide composition of the present invention or the authentication method of various active compound are also provided.

In another embodiment of the present invention, provide the extracting method of the American wormseed oil that contains high-load with mite extremely and/or insecticidal activity.

The method of the Chenopodium vegetable oil that can produce high quantity and/or quality from the Chenopodium plant also is provided in another embodiment.Certainly, the known this oily preparation method of the those of ordinary skill in field related to the present invention is also applicable to the plant and the special requirement of other kind.

The invention still further relates to the method that infects due to a certain regional miticide of preventing, this method comprises that the present composition with effective dose is applied to this zone.

In addition, the composition for preventing and controlling that the present invention relates to plant extracts and/or contain ascaridole infects the purposes of plant mites class.This method comprise with kill the extract of the present invention of mite amount and/or composition with by plant mites class (or pathogenic insect) plant of infecting or the one or more parts of the plant that is subject to infect or organize the step that contacts.About the non-limiting example of the tissue of plant and part comprises seed, seedling and plant itself.

The invention still further relates to the processing method in the zone of being infected, comprise that the present composition or the extract that will kill the mite effective dose are infected regional the contact with quilt by the mite class.

The applicant proposes to use the chenopod extract as acaricidal the first.And before the present patent application, also nobody considers to use and/or confirms that the chenopod extract is as miticide.In addition, the applicant is to use and contains the ascaridole compound compositions as acaricidal the first.

Used at this, term " mite " broadly is meant plant mites.Similarly, term " mite class " broadly is meant mite class or the phytophagous mites that infects plant.

Difference between herbaceous plant extract and the woody plant extract herein is: promptly the woody plant extract is meant the extract that is extracted by woody plant (tree), and the herbaceous plant extract is the extract that derives from non-tree plant.

(as the terpene of enumerating in the following table) except as otherwise noted, ascaridole and different ascaridole represented herein in term " ascaridole ".

Term used herein " zone " broadly is meant and is infected the place that maybe infected by the mite class by the mite class.

Though can be changed by the zone or the place of mite class and/or infestation by insect and the available present composition or method control, in the preferred embodiment of the invention, this zone is a live plant, especially the plant crop.The non-limiting example of above-mentioned plant comprises corn, cotton, flowers, fruit tree, vegetables, tobacco etc.

Term " is settled down " or the association of insect and material, zone or place (for example organism or tissue) represented in similar term, and insect therefrom obtains nutrition.

" control " of term insect (mite class and/or insect) broadly is meant the growth of target pest and/or active remarkable reduction.

The applicant shows between terpene ascaridole content in the plant extracts and the α terpinene content and is negative correlation.The applicant shows that also its acaricidal activity of plant extracts that contains high-caliber more α-terpinene is low more.

The applicant also is to use the herbaceous plant extract as acaricidal the first.

The applicant still confirm ascaridole in the plant extracts and/or with the existence of the compound of ascaridole fractionation with kill proportional the first of mite/insecticidal activity.And, when the fractionation chenopodium ambrosioides extract, confirm that acaricidal activity is proportionate with ascaridole and/or with the existence of the compound of ascaridole fractionation.Therefore, the invention provides and have the potential authentication method that mite extremely is provided or kills other plant of mite and insect-killing extract, promptly by measuring the ascaridole in this plant and/or can being converted into the compound of ascaridole and/or with the content of the compound (or multiple compound) of ascaridole fractionation.

In the preferred embodiment, the present composition comprises at least a terpene.The non-limiting example of findable these terpenes comprises that laurene, α-terpinene, p-cymene, cineole, limonene, γ-terpinene, t-are to -2 in the present composition, 8-diene-1-alcohol, c-be to -2,8-diene-1-alcohol, carveol, ascaridole, different ascaridole, carvol, t-cinnamic acid, n-nonanoic acid, thymol and carvacrol.

Monoterpene and sequiterpene are the key components in the essential oil.The monoterpene of being derived by 2 isoprene units is the compound (C that contains 10 carbon atoms ₁₀) and be the compound (C that contains 15 carbon atoms by the sequiterpene that 3 isoprene units are derived ₁₅).Because their low boiling, it is essential oil that monoterpene and sequiterpene all belong to the steam distillation cut.Known these compounds are present in the fragrance, volatile essential oil of many plants, and above-mentioned plant comprises peppermint, pine tree, cypress, oranges and tangerines, eucalyptus and aromatic crop.For example, laurene is present in the essential oil of basyleave and hop.Carvol is many a kind of (Brielmann 1999) can odoriferous component of extracting from Caraway seed (Carum carvi).

The insecticidal action of monoterpene (mechanism) comprises neurotoxicity and insect growth regulator, IGR (IGR) activity and insect-repellent and fumigant insect killing activity (Coats 1994).

In addition, the invention provides the composition of killing undesired plant mite class, said composition comprises by natural essential oil that obtains in the plant and suitable carrier, wherein essential oil account for composition about 0.125% to about 10%, and wherein essential oil comprises at least a compound that is selected from ascaridole, different ascaridole, α-terpinene, p-cymene, limonene, thymol and carvacrol.

In addition, the invention provides control and infect the method for mite class of plant, this method comprises that the essential oil composition that contains that will contain the plant acaricidal composition that kills the mite effective dose of at least a compound that is selected from ascaridole, different ascaridole, α-terpinene, p-cymene, limonene, thymol and carvacrol and suitable carrier is applied to needs and prevents and treats the place.

And, the invention provides the method that the plant mites class is infected in a kind of control, this method comprises the place that the present composition is applied to the needs control.In addition, also provide a kind of method of preventing and treating the mite class, this method comprises that the present composition that will kill the mite effective dose contacts with mite class or their habitat.

Therefore found to derive from the floristic extract in North America to T.urticae Koch model system (Tetranychus urticae Koch: Tetranychidae) have toxicity.Determined the phenology period (Jackson et al.1994) of Several Factors such as plant, the water content % (Chialva et al.1983) of results material, the plant part that selection is used to extract (Jackson et al.1994, above; Chialva et al.1983, above), and extracting method (Perez-Souto 1992) is the possible cause that causes extract chemical composition and effect to change.

Therefore, a large amount of North America floristics extracts demonstrate significant acaricidal activity.Particularly, the Chenopodium plant extracts, more particularly chenopodium ambrosiodies and Central Asia Artemisia santonica and tanacetum parthenium extract also show acaricidal activity.

As described in the embodiment of the invention, can use partially purified extract.In addition, extract of the present invention preferably with carrier or mixing diluents (carrier of selection or thinner should make it can not reduce the acaricidal activity of plant extracts significantly) to obtain miticide composition.More preferably, extract mixes with carrier, most preferably with emulsifier.

In preferred embodiments, use the composition that now mixes the ready-to-use formulation form.With the oil extract stereometer, this existing mixing is now counted 0.125%-10% with the ultimate density of composition with the oil extract volume, preferably counts 0.25%-2% with the oil extract volume.Certainly, those skilled in the art can change above-mentioned concentration according to special requirement.For example, use the composition of 95% concentration and before application, diluting.The composition of the cream preparation concentrate form of the oil extract that by volume contains 5%-50% is provided in another embodiment.Actual operator will be adjusted concentration does not have phytotoxicity so that it has acaricidal activity.Preferably, extract of the present invention and composition have acaricidal activity, but do not have phytotoxicity and mammal is not had toxicity.Also preferably, extract and composition also have the advantage of insecticidal activity.

Miticide composition of the present invention and kill mite and the superior part of Pesticidal combination preferred embodiment is their Environmental compatibility or environmental safety.

The present composition can mix with a large amount of known carriers and/or auxiliary agent.Preferred vector is an inertia.The non-limiting example of these carriers and/or auxiliary agent comprise be used to form emulsion, aerosol, spray or other liquid preparation, solvent, thinner and/or the surfactant of dust pulvis or solid pharmaceutical preparation.The non-limiting example of solvent and thinner comprises that water, aliphatic series and aromatic hydrocarbon (being dimethylbenzene or other petroleum distillate) and alcohol are as ethanol.Surfactant can be the surfactant of anionic, cationic or nonionic type.Can also comprise stabilizing agent, spices, colouring agent and antioxidant.The quantity of these carriers that the present invention uses and type all are conventional uses the in field of pesticides and the particularly miticide composition.For specific embodiment, it may be favourable that slow releasing composition is provided.This preparation is particularly conducive to and prevents infecting or preventing infecting again of this zone of a certain zone.The non-limiting example of sustained release preparation comprises the present composition that seal with capsule or granulation.

Be used for extract of the present invention comprises anion and nonionic emulsifier at the emulsifier of water solubilising admixture.In the particular, these emulsifier are the isopropyl amine salt of alkyl benzene sulphonate or analog and the octylphenol ethoxylate that has 9 moles of ethylene oxide or equivalents.The percentage of the common usage amount of anion emulsifier is that the percentage of 0.125%-5% and the common usage amount of nonionic emulsifier is 0.125%-5%.

The percentage that makes use-case in the preparation of the present invention is that the example of the anion emulsifier of 0.125%-5% includes, but not limited to anion emulsifier listed in the table 1.Table 1: the operable anion emulsifier of the present invention

The trade name of anion emulsifier	The chemical name of anion emulsifier	Supplier
			Rhodacal?DS-10	Neopelex	Phone?Poulenc，Cranbury?NJ
Calfax?DB-45	The dodecyl diphenyl ether sulfonate	Pilot?Chem.Co.Santa?Fe?Springs?CA
			Stepanol?DEA	The diethanol amine lauryl sulfate	Stepan?Co.，Northfield?IL
Aerosol?OT-75	Sodium dioctyl sulfosuccinate	Cytec?Industries?Inc.，Morristown?NJ
			Rhodacal?A246L	(C14-C16) alkene sulfonic acid sodium	Rhone?Poulenc，Cranbury?NJ
Rhodafac?RE?610	Nonylbenzene-9 phosphate	Rhone?Poulenc，Cranbury?NJ
			Rhodapex?CO-436	Nonyl phenol (4EO) ammonium sulfate	Rhone?Poulenc，Cranbury?NJ

The non-limiting example of operable nonionic emulsifier in the preparation of the present invention (percentage is 0.125%-5%) is in table 2.Table 2: the operable nonionic emulsifier of the present invention

The trade name of nonionic emulsifier	The chemical name of nonionic emulsifier	Supplier
			Igepa?CO-887	Nonyl phenol ethoxylate (30 moles)	Phone?Poulenc，Cranbury?NJ
Macol?NP-9.5	Nonyl phenol (POE 9.5) ethoxylate	PPG?Industries，Gumee?IL
			Igepal?CO-430	Nonyl phenol ethoxylate (4 moles)	Phone?Poulenc，Cranbury?NJ
Rhodasurf?ON-870	Oleyl alcohol POE 20	Phone?Poulenc，Cranbury?NJ
			Alkamuls?EL-719	Castor oil (40 moles of EO)	Phone?Poulenc，Cranbury?NJ
Alkamuls?EL-620	Castor oil (30 moles of EO)	Phone?Poulenc，Cranbury?NJ
			Alkamide?L9DE	Lauramide DEA	Phone?Poulenc，Cranbury?NJ
Span?80	Dehydrating sorbitol monooleate	ICI?Surfactants?Wilmingion?DE
			Tween TM?80	POE 20 dehydrating sorbitol monooleates	ICI?Surfactants?Wilmington?DE
Alkamuls?PSMO-5	POE anhydrosorbitol (5) monoleate	Phone?Poulenc，Cranbury?NJ
			Atlas?G1086	Anhydrosorbitol six oleates	ICI?Surfactants?Wilmington?DE
Tween TM?20	POE 20 sorbitan monolaurates	ICI?Surfactants?Wilmington?DE

Above listed emulsifier and non exhaustive; The ternary mixture that can also use emulsifier is with the stable droplet emulsus agent of the plant extracts that obtains to have required physicochemical property.Preparation is as other stable droplet emulsus agent of killing mite/Pesticidal combination or slightly to drip the emulsus agent formulation be conspicuous for any those of ordinary skill in this area.The those of ordinary skill of correlative technology field of the present invention can be adjusted this composition as the function of need processing region.Should be realized that active component of the present invention can be usually used in promoting acceptable carrier known on the agricultural of active component dispersiveness and surfactant blending.Final preparation and the method for application that should recognize miticide composition of the present invention and extract may influence its activity.Certainly, the invention provides the assay method of dissimilar composition miticidal effects.

Having added percentage in the preferred embodiment of preparation of the present invention is spreading agent and the sticker of 0.05%-10%.Spreading agent/the sticker that is used for preparation of the present invention includes but not limited to be positioned at the Schercoat P110 that the Scher Chemicals of Clifton NJ provides, and Pemulen TR2 that the BF Goodrich of BrecksvilleOH provides and the Carboset 514H that is also provided by the BF Goodrich of that is positioned at Brecksville OH are provided.

Using of the present invention now mixing under the ready-to-use formulation situation, complementing to 100% volume, and in the missible oil concentrate, carrier can be THFA to water as carrier, or isopropyl alcohol.According to the concentration of extract, add the Isopar of 50-90%.

Preferably, preparation has good physicochemical property, and is stable after room temperature, 37 and 50 is stored 6 months.

The present invention now mixes the preferred embodiment of ready-to-use formulation and lists in the table 3.Table 3: now mix ready-to-use formulation

Product	Content in the preparation (%)
		The Chenopodium plant extracts	1.0
Rhodacal?IPAM	0.83
		Igepal?CA?630	0.5
Pemulen?TR2	0.05
		Propane diols	2.0
Water	Complement to 100%

The preferred embodiment of emulsion of the present invention is listed in the table 4.Table 4: emulsible concentrate formulation

Product	Content in the preparation (%)
		The Chenopodium plant extracts	25
Rhodopex?CO-436	2.5
		Igepal?CO-430	2.5
IsoparM	70

Be appreciated that and contain at least a other active component in oil of the present invention and the composition.This active component can be the known compound (for example other terpene) with insecticidal activity (killing mite or other activity).Be understandable that above-mentioned at least a other active component and reactive compound of the present invention can produce or not have synergistic effect.

Oil of the present invention and composition can be used on the dissimilar environment of control, comprise insect in family, agricultural and the gardening environment/infect.

Be understandable that and infect according to ambient conditions, by the mite class or be subject to the regional situation that it infects, can corresponding adjustment composition.

The invention also discloses the experiment that obtains kind, harvesting approach and the distillating method of the highest yield oil in order to decision.Plant leaf in period of living in, flower or seed stage when also having set up in order to the oil production of relatively chenopodium ambrosiodies kind 1 and kind 2 and results, the water content of vegetable material before the distillation, promptly fresh (＞75%), the experiment of the oily yield that obtains under the various different conditions such as extracting method of wilting (40-60%) or dry (＜20%) and use.

Use the herbaceous plant extract to substitute another advantage of woody plant extract as the present invention, the actual conditions that the present invention includes be since herbaceous plant sooner and be easier to growth and therefore make harvest yield ripe more than 1 year one.

Extracts essential oil is to adopt the method can produce volatile component and other composition to carry out, and the products known as " extract " that obtains when all plant parts of choosing outside digging up the roots (Duerbeck1993).Usually essential oil is not single composition.Their difference is formed the difference that shows as on its physicochemical property.For most applications, all these components all are volatilizable and be immiscible relatively in water in water vapour, and this characteristic makes that separating said components from the distillate mixture becomes possibility.There is multiple different steam distillation method at present.The present invention chooses following a few method at the purpose of extracting the chenopodium ambrosiodies essential oil: water distillation method (DW), directly steam distillation method (DSD) (Duerbeck, 1993).Other extracting method such as solvent extraction and microwave householder method (MAP are also disclosed except that distillating method ^TM).Those of ordinary skills also can adopt other known method.

T.urticae Koch, formal name used at school Tetranychus urticae are considered to the model test target of miticide biologicall test test.The various toxicologic methods that are used to test miticide and preparation activity thereof all reported in several pieces of articles, and most methods uses T.urticae Koch as the test species.Ebeling and Pence (1953), Ascher and Cwilich (1960), Dittrich (1962), Lippold (1963), Foot and Boyce (1966) and Anonymous (1968) have assessed the whole bag of tricks and have been set up the standard of the accuracy of judgement the whole bag of tricks by Busvine (1958), be result's accuracy, reproducibility and operation rationally easy, all methods all use the spider mite as test model.Recently, the spider mite also usually is used in and checks and measure (Walker etal.1973 in the research method of resistance; Anon.1974; Dennehy et al.1983; Dennehy et al.1992).

Other purpose of the present invention, advantage and characteristic will become more clear by the description of reading following non-limiting preferred embodiment, but these embodiments are illustrative, should not be construed as to limit the scope of the invention.

Preferred embodiment is described

Therefore the invention provides the plant resource miticide that overcomes the relevant a large amount of shortcomings of known miticide.More specifically, the essential oil of various plants shows significant acaricidal activity.In addition, the present composition shows significant insecticidal activity to the pest species of several serious harm cultivated plants.

The invention provides and compared several different preparation methods of essential oil.In a specific embodiment, the plant resource composition that has confirmed to contain chenopodium ambrosioides extract has very strong acaricidal activity and compares with the known mite compound that kills.

The present invention is further explained in detail by following non-limiting embodiment. Embodiment 1

The chenopodium ambrosiodies breed selection

Chenopodium ambrosiodies is grown in temperate zone or subtropical zone.Aellen and Just (1943) and Voroshilov (1942) are divided into several subspecies and type with chenopodium ambrosiodies.Yet, according to the sorting technique of prior art, the feature (being the shape, inflorescence types of down, seed size, the perianth part of stem and leaf and full of leaves, blade shape, leaf margin feature) that is used for the differentiation type can not be classified to the kind that the present invention uses.

Be this paper when being called the plant of kind 1 and 2 in using Aellen and Just botany, the kind 1 immediate C.ambrosioides of being var.suffruticosum (Wild.) Alellen and kind 2 are immediate to be C.ambrosioides var.Ambrosioides.In the sort research of upgrading, the botanist does not have approval by the sorting technique of telling divergence type in the complicated taxonomic hierarchies of chenopodium ambrosiodies.For reaching the object of the invention, no matter whether these kinds distinguish like this, confirmed advantageously to select the to have specific trait kind of (seed of short period, the high quantity of results, littler and blade still less etc.) is to obtain the highest oily yield. Embodiment 2

Crop genetics

Plant successfully has been grown in the area, temperate zone and has formulated the plant planting mode, and the per kilogram vegetable material can obtain the more oil with height ratio active component of a large amount thus.Plant began to grow in the greenhouse and transplanted to the field the middle ten days in may the first ten-day period of the March.This mode allows vegetable material that arranged at least twice main harvest time, gather in the crops at every turn average 10-20 metric ton (MT)/hectare and per season the 20-40MT/ hectare and gross yield be the 20-100L/ hectare.

In the cultivation process of the present invention, growth has the result of the chenopodium ambrosiodies of several kinds and these results tests to cause finding carrying out when this crop grew to seed stage in the crop production first time results in 60 days to carry out after-crop (mid-August is to the last ten-days period) in (mid-July) and 40 days and gather in the crops (last ten-days period in September) for the third time.As mentioned above, the evaluation to different cultivars is uncertain.Therefore the selection of two most worthies is confirmed as kind 1 and kind 2.

Determine the reproduction speed of plant after the results and after-crop for the first time by the test of different cutting height, discovery cutting height (10cm but be no more than 30cm) at least can obtain quick and dense plant growing, thereby blooms in several days after results.Preferred this quick regeneration method is to gather in the crops the vegetable material of maximum in the colder area, temperate zone in for example southwest, Quebec.By chromatography different fractionation modes and method are measured oily yield and are measured oil ingredient by Study of Chromatographic Analysis, these caused determining preferred kind, the vegetation period and results after the plant treatment measure to obtain best oil yield and quality. Embodiment 3

The feature of chenopodium ambrosiodies essential oil

Contain multiple compound in the essential oil extract of plant such as chenopodium ambrosiodies.Two kinds of monoterpenes, ascaridole and different ascaridole are the chief components (being respectively 10-70% and 3-20%) of essential oil.Yet even hour also can observe biologically active when the content of these monoterpenes, this shows that gross activity comes from the existence of the complex mixture of all cpds.Other monoterpene such as p-cymene, limonene and α-terpinene also are key components and make oil have insecticidal activity.Table 5 has been listed the main compound of identifying by gas-chromatography (GC-FID); Reading is to determine by the DB-WAX post.Table 5: chenopodium ambrosiodies seed, kind 1﹠amp; The percentage composition of compound in 2 the oil extract

The compound that exists	Concentration range in the oil (%)
		Laurene	0.1-3
α-terpinene	5-60
		P-cymene	5-30
Cineole	0.1-3
		Limonene	1-20
γ-terpinene	0.25-5
		T-is to -2,8-diene-1-alcohol	0.25-5

C-is to -2,8-diene-1-alcohol	?0.25-5
		The 2-baras camphor	?0.1-3
The 4-terpineol	?0.1-3
		P-cymen-8-ol	?0.1-2
Carveol	?0.1-2
		Ascaridole	?10-70
Carvol	?0.1-2
		Cinnamic acid	?0.1-2
?Acide?pelargonique	?0.1-1
		Different ascaridole	?3-20
Thymol	?0.25-10
		Carvacrol	?0.25-10

Whether each compounds content (or %) can dry or fresh with the vegetative period of the plant part of the chenopodium ambrosiodies kind that adopts, results, plant and vegetable material in the total amount of the oil that extracts and the oil, the type of the extracting method of time in season, weather vegetatively and the use of gathering changes.

Therefore the inventive method can be obtained the type of required oil and make extract satisfy specific demand by adjusting the oily output of influence and the various parameters of quality by those of ordinary skills. Embodiment 4

Water distillation extracting method (DW)

The chenopodium ambrosiodies vegetable material all is immersed in the water, comprises that by direct heating the vegetable material of loose filling and jar to the water of water seethe with excitement.Vapour jacket around being wrapped in jar or steam pipe coil also can be used for adding hot water.The main feature of DW is that vegetable material always contacts with the water of boiling and the volatile component of plant is released in the water, reclaims in distillation and cooling back. Embodiment 5

Direct steam distillation extracting method (DSD)

According to this method, the chenopodium ambrosiodies vegetable material more fills in the jar consolidation, as long as guarantee that water vapour can pass through vegetable material unobstructedly.Boiler or steam generator that water vapour feeding speed can be effectively controlled in employing produce water vapour by the outside.Must be that the water vapour of fractional wettability contacts oozing out of the essential oil that causes cell membrane and forms mixture with water vapour with vegetable material.These essential oil components are volatilizable being lower than under 100 degrees centigrade the temperature condition, therefore can be along with the steam distillation through vegetable material.When cooling device by the jar outside of steam and volatile materials, be condensed then, then when oil fall to the water collected from condenser below the time, recovered oil. Embodiment 6

Organic solvent extraction

Organic solvent also can be used for extracting the organic soluble compound that exists in the essential oil.The non-limiting example of these organic solvents comprises methyl alcohol, ethanol, hexane, carrene.These extracting method can use dissimilar extractors such as Soxlet, and the glass reflux that Soxlet is special wherein extracts material 18 hours 80.The yield that uses methyl alcohol, ethanol, hexane and adopt the result of the test acquisition 0.5-3% of Soxlet device.Use solvent to carry out more massive extraction (promptly commercially producing) and need special pressue device.This high combustibility and explosion hazard make that also operation is very also complicated so cause security feature with high costs. Embodiment 7

More selected parameter is to the influence of the yield of the oil that obtained by chenopodium ambrosiodies

For measuring (1) kind (kind 1 and 2); The water content % of material when (2) distilling (fresh, wilting and dry); The growth period (leaf, flower and seed) of plant when (3) gathering in the crops; And can obtain the highest oily yield under that a kind of condition in (4) distillating method (DW and DSD), tested 30 combinations of different variablees altogether, and each makes up repetition 3 times, 90 still-process altogether.The yield that each variable obtains is summarised in (table 6) in the following table.Be understandable that from being used for the various combination mode that provides of this test, Ji Zai mean value is only represented to pass through the yield that different variablees obtain herein.When consider all 30 combinations as a result the time, obtain the highest yield (greater than 0.5%; Table 6) combination is to use the yield of DW method from the seed stage acquisition of kind 2.Fresh and wilting material obtains higher yield than drying material.Other test has confirmed these results.DSD obtains lower yield than DW; Yet DSD should consider to be used for commercialization and extract mode of operation, because this method is more more economical and labour intensity is lower than DW cost.Table 6: the water content % of different cultivars, different distillation material, the average yield (%) of the oil that different growth period of plant and different distillating methods obtain during results

Variable	Average yield (%)	Standard deviation	The distillation number of times
				Distillating method
DSD	0.1673	0.108	45
				DW	0.5952	0.36	45
Humidity percentage %
				Fresh	0.3686	0.336	30
Wilt	0.4684	0.393	30
				Dry	0.3068	0.299	30
The plant stage of living in

Leaf	0.2403	0.154	30
				Flower	0.3057	0.275	30
Seed	0.5273	0.421	30
				Kind
Kind 1	0.264	0.2316	45
				Kind 2	0.5571	0.4064	45

Select kind 2 to be used for oily production, because it can obtain the highest oily yield; This kind also has shorter growth cycle (being less than 60 days after the transplanting), can allow to carry out each season once above results.

Be understandable that in specific embodiments, various combination can be more suitable in special demand.In addition, as follows, can not be with yield as unique important indicator. Embodiment 8

Determine to produce and have the condition of the oil of high acaricidal activity %

Adopt various tests to determine to obtain plantation and the results technology and the distillating method of optimum oil yield.30 extracts of above-mentioned campaign are carried out each type of compounds and the relative amount of chromatography to determine to exist in the oil.

Carry out the chromatographic isolation of organic molecule.Analyze essential oil and extract by the capillary gas chromatography (GC) that flame ionization detector (FID) is housed.Use Varian 6000 series Vista to carry out GC, calculate peak area by Varian DS 654 integrators.Use the fused silica column of SPB-1 (30mX0.25mm , 0.25 μ m) and Supelcowax (30m, 0.25mm , 0.25 μ m).Compound in the sample comes out at different time wash-out from post, minute to be unit representation (retention time), then with the known standard object of reference relatively, identify each compound.When GC-FID can not clearly identify some compound, adopt mass spectrometry (MS) that the mass spectrum of each compound and the mass spectrometric data storehouse of known compound are compared.

For of the bioactive effect contribution of assessment ascaridole, will compare (reading from the Supelcowax post of GC-FID) according to the percentage composition of the ascaridole in the different extracts of above-mentioned test method (being developmental stage, kind, the distillating method of water content, plant) preparation to oil.The ascaridole of acquisition and the percentage composition addition of different ascaridole are obtained total ascaridole content.For the situation of change of each processing, when the difference of ascaridole content at least greater than 10% the time, think that then this variation is significant.So for the different moisture content vegetable material, (fresh, wilt drying; Table 7) with different distillating methods (DSD, DW; Table 9) percentage composition of each compound does not have significant difference between the oil of Ti Quing.Ascaridole content is lower in the kind 1 (table 10), but this content still very high (38.5%).Yet the leaf phase (9.8%) of this plant is than the ascaridole lower (table 8) of florescence (64.8%) and seed stage (55.4%) acquisition.Table 7: the relative amount (%) of each compound in the essential oil that extracts by vegetable material under different moisture content (fresh, as to wilt and the drying) condition

The relative amount of every kind of compound
				Compound	Fresh	Wilt	Dry
P-cymene	10.4	11.9	2.9
				Limonene	0.3	0.9	2.6
Ascaridole	46.5	50.9	51.9
				Different ascaridole	8.9	9.8	10.4
Thymol	1.1	0.4	2.2

Table 8: the relative amount (%) of each compound in the essential oil that the vegetable material that different developmental phases (leaf, flower and seed) is gathered in the crops extracts.Use the fresh plant material of kind 1 and adopt the DSD method to extract.

The relative amount of every kind of compound
				Compound	Leaf	Flower	Seed
P-cymene	50.6	11	10.4
				Limonene	2.09	0.2	0.3
Ascaridole	6.7	53.9	46.5
				Different ascaridole	3.1	10.9	8.9
Thymol	0.3	1.5	1.1

Table 9: by the relative amount (%) of each compound in the essential oil of DSD and the extraction of DW method.Use the fresh plant material of the seed stage of kind 1.

The relative amount of every kind of compound
			Compound	DSD	DW
P-cymene	10.4	10.8
			Limonene	0.3	0.2
Ascaridole	46.5	42
			Different ascaridole	8.9	8.1
Thymol	1.1	2.7

Table 10: by the relative amount (%) of each compound in the essential oil of kind 1 and 2 plant extract.Use the fresh plant material of seed stage and pass through the extraction of DW method.

The relative amount of every kind of compound
			Compound	Kind 1	Kind 2
α-terpinene	33.8	14.7
			P-cymene	11.6	8.1
Limonene	0.4	5.5
			Ascaridole	38.7	52.2
Different ascaridole	8.4	9.2

Thymol

2.5

Test representative is above-mentioned then is that the biologically active of 30 extracts of the different disposal of the growth and development stage of kind, water content, vegetable material and distillating method and combination exerts an influence with the content that the determines whether ascaridole toxic degree to test organism.All are given birth to test and test and all use T.urticae Koch, formal name used at school Tetranychus urticae (referring to above, a kind of model system that is used for the miticide biologicall test).

Regrettably, be not tangible positive correlation in the extract between the relative amount of ascaridole and the acaricidal activity.In other words, the extract that has the highest ascaridole percentage composition after measured might not be the most highly active mite extract that kills.Therefore, as if though ascaridole is relevant with the acaricidal activity of oil of the present invention or composition, other compound is necessary to the performance acaricidal activity also.In fact, the ascaridole of purifying does not show the remarkable acaricidal activity shown in the plant extracts that contains ascaridole of the present invention. Embodiment 9

Now mix existing with (RTU) mite-killing preparation

Preparation contains as existing the mixing of the Chenopodium plant extracts of active component now uses (RTU) aerosol bomb.In preferred embodiments, said preparation contains Chenopodium plant extracts, emulsifier, spreading agent and sticker and the carrier of 0.125%-10%.The example of operable nonionic and anion emulsifier as previously mentioned in the preparation of the present invention.

1 A fluorescent / sodium lighting Saver, including the power supply control board (202), Lack of trust of source (203), work status display device (204), thyristor SCR module (205), three-phase AC contactor module (206), the power supply control board (202) with the first Four transformers (179) and with two-pin socket (5 ') of cable, lack of phase signal source (203) Containing first transformer (175), a second transformer (176), a third transformer (177), first Fuse (181), a second fuse (182), third fuse (183) and with a four-pin plug Block (2 ') of the connector, the working state display device (204) comprising a first switch SP3T (184), a second single-pole three-throw switch (185), fan (189), the first indicator (186), The second indicator (187), a third indicator (188), relays (CJ ') and with two-pin plug Block (3 ') of cable, the first single-pole three-throw switch (184) and a second single-pole three-throw switch (185) coaxial connections, the first single-pole three-throw switch (184), a second single-pole three-throw switch (185), the first indicator (186), the second indicator (187) and a third indicator (188) Mounted on a panel, the thyristor SCR module (205) comprising a first silicon tube (172), Second thyristors (173), third thyristors (174), with a four-pin socket (8 ') is even Wiring, with four-pin socket (9 ') of cable with four-pin socket (10') of cable, Three-phase line input, three-phase line output, temperature control tube (178) and with two-pin socket (6 ') Connecting line, three-phase AC contactor module (206) with three-phase AC contactor (180), Three-phase line input, four-pin socket (1 '), three-phase line outputs and relays (CJ') The normally open contact (CJ), characterized in that the 380V three phase lines UA, UB, UC, respectively, Three-phase AC contactor (180) of the three-phase line input, four-pin socket (1 ') of the plug Sleeve 11 ', 12', 13 'and thyristor SCR module (205) in the third thyristors (174), Second thyristors (173), first thyristors (172) connected to the positive, four-pin socket (1 ') of the insert sleeve 14' N and connected with the zero line, three phase output lines L3, L2, L1, respectively, Three-phase AC contactor (180) of the three phase output terminal and the third thyristors (174), Second thyristors (173), first thyristors (172) connected to the negative, the thermostat tube (178) Fixed to the thyristor SCR module (205) of the radiator, temperature control tube (178) of the two Outputs respectively, and two-pin socket (6 ') of the insert sleeve 61' and 62 'connected, three phase 380V Line UA, UB, UC any one phase and neutral line N, respectively, and job status display device (204) The input terminal U 'and N' connection, the first SP3T switch (184) and a second pole of the knife Three throw switch (185) connected to the blade, a second indicator (187) is connected across the input terminals U 'and N 'between the fan (189) and fourth transformer (179) of the primary winding connected in parallel across In the second single-pole three-throw switch (185) and the input terminal of the next toss N 'between the second pole three Throw switch (185) and the first on throwing SP3T switch (184), respectively, and the next throw two core Outlet (3 ') of the insertion sleeve 32' and 31 'is connected, a first indicator (186) and the relay (CJ') In parallel across the second single-pole three-throw switch (185) and the input terminal on throwing N 'between Relay (CJ ') normally open contacts (CJ) across the two-pin socket (3') of the insert sleeve 32 ' And 31 ', the third indicator (188) is connected across the first transformer (175) primary Ends of the coil of the first transformer (175), a second transformer (176), a third transformer (177) One end of the primary coil through the first fuse (181), a second fuse (182), The third fuse (183) and three power outputs L1, L2, L3 connected to the first fuse (181), a second fuse (182), a third fuse (183) the other end of the zero line N Connection, the first transformer (175), a second transformer (176), a third transformer (177) One end of the secondary winding and the four-pin socket (2 ') of the insert sleeve 24' is connected, first transformer (175), a second transformer (176), a third transformer (177), another secondary coil Ends with four-pin socket (2 ') of the insert sleeve 21', 22 'and 23' connected to the fourth transformer (179) both ends of the secondary winding and two-pin socket (5 ') of the insert sleeve 51' and 52 ' Connection, characterized in that it further comprises control means (200) and the module phase drive plate (201), Control means (200) by a resistor: a first resistor (317), a second resistor (318), the third Resistor (319), a fourth resistor (320), a fifth resistor (321), a sixth resistor (322), Seventh resistor (323), the eighth resistor (324), the ninth resistor (325), the tenth resistor (326), Eleventh resistor (327), the twelfth resistor (328), thirteenth resistor (329), the fourteenth Resistor (330), fifteenth resistor (331), the sixteenth resistor (332), seventeenth resistor (333), Eighteenth Resistance (334), nineteenth resistor (335), twenty-resistance (336), twenty- A resistor (337), the twenty-second resistor (338), the twenty-third resistor (339), twenty- Four resistors (340), the twenty-fifth resistor (341), the twenty-sixth resistor (342), twenty- Seven resistor (343), the twenty-eighth resistor (344), the twenty-ninth resistor (345), the thirtieth Resistor (346), the thirty-first resistor (347), thirty-second resistor (348), the thirty-third Resistor (349), the thirty-fourth resistor (350), the thirty-fifth resistor (351), the thirty-sixth Resistor (352), the thirty-seventh resistor (353), 38th resistor (354), the thirty-ninth Resistor (355), fortieth resistor (356), forty-first resistor (357), forty-second electric Resistance (358), the forty-third resistor (359), forty-fourth resistor (360), the forty-fifth power Resistance (361), forty-sixth resistor (362), forty-seventh resistor (363), the forty-eighth power Resistance (364), forty-ninth resistor (365), fiftieth resistor (366), fifty-first resistor (367), fifty-second resistor (368), the fifty-third resistor (369), the fifty-fourth resistor (370), fifth resistor (371), the fifty-sixth resistor (372), fifty-seventh resistor (373), fifty-eighth resistor (374), exclusion: The first exclusion (375), second exclusion (376), The third exclusion (377), fourth exclusion (378), fifth exclusion (379), Variable resistor: a first variable resistor (380), a second variable resistor (381), a third variable Resistance (382), a fourth variable resistor (383), the fifth variable resistor (384), sixth variable Resistance (385), seventh variable resistor (386), capacitance: a first capacitor (387), a second capacitor (388), a third capacitor (389), a fourth capacitor (390), the fifth capacitor (391), sixth Capacitor (392), seventh capacitor (393), eighth capacitor (394), ninth capacitor (395), Tenth capacitor (396), eleventh capacitor (397), the twelfth capacitor (398), thirteenth electricity Capacity (399), the fourteenth capacitor (400), fifteenth capacitor (401), sixteenth capacitor (402), Seventeenth capacitor (403), eighteenth capacitor (404), nineteenth capacitor (405), twenty- Capacitor (406), Diodes: a first diode (407), a second diode (408), a third diode (409), Fourth diode (410), fifth diode (411), sixth diode (412), seventh two Diode (413), the eighth diode (414), the ninth diode (415), the tenth diode (416), Eleventh diode (417), the twelfth diode (418), thirteenth diode (419), Fourteenth diode (420), fifteenth diode (421), sixteenth diode (422), Seventeenth diode (423), eighteenth diode (424), eighteenth diode (424), Nineteenth diode (425), twenty-diode (426), the twenty-first diode (427), Twenty second diode (428), the twenty-third diode (429), the twenty-fourth diode (430), the twenty-fifth diode (431), the twenty-sixth diode (463), the twenty-seventh Diode (464), the twenty-eighth diode (465), Zener diode: The first regulator diode Tube (432), second Zener diode (433), a third Zener diode (434), fourth steady Voltage diode (435), Manifold: first manifold (436), a second manifold (437), The third manifold (438), fourth manifold (439), fifth manifold (440), sixth episode Into blocks (441), seventh manifold (442), eighth manifold (443), ninth manifold (444), Tenth manifold (445), three-terminal regulator: The first three terminal regulator (446), SCR: The first silicon (447), light-emitting diodes: a first light emitting diode (448), rectifier bridge: A first rectifier bridge (449), the relay: a first relay (450) composed of the phase shift drive module Plate (201) by the resistance: the fifty-ninth resistor (451), sixty-resistance (452), p. Sixty-one resistor (453), the sixty-second resistor (454), the sixty-third resistor (455), p. Sixty-four resistor (456), Zener diode: Fifth Zener diode (457), sixth regulator Diode (458), seventh Zener diode (459), eighth Zener diode (460), p. Nine Zener diode (461), tenth Zener diode (462), transistor: a first transistor (463), a second transistor (464), a third transistor (465), the fourth transistor (466), Fifth transistor (467), sixth transistor (468), pulse transformers: a first pulse transformer Device (469), a second pulse transformer (470), the third pulse transformer (471), with Control means (200) for electrical connection, two-core plug (5) of the pins 51 and 52 respectively, and First rectifier bridge (449) 2 and 3 pin connector, first rectifier bridge (449) of a foot, the first Nineteen capacitor (405) of the cathode, the first three terminal regulator (446) of a foot, seven-pin plug (4) of the pin 47 and then, the first three terminal regulator (446) 2 feet, twenty-capacitance (406) The positive electrode, the thirteenth resistor (329) of the upper end of the twenty-eighth resistor (344) The upper end of the thirty-resistance (347) of the upper end of the seventeenth resistor (333) of the upper, Sixteenth resistor (332) of the upper end of the thirty-fourth resistor (350) of the upper end of the thirty-fifth Resistor (351) of the upper end, a second resistor (388) of the right side, the ninth manifold (444) 5 feet, the first semi-variable resistor (380) of the upper end of the forty-ninth resistor (365) on End of the tenth manifold (445) 4,8 feet, fifty-third resistor (369) of the upper end of the A twenty-first diode (427) of the anode, the first relay (450) of the upper end of the seven-pin plug Head (4) of the pins 4,6, fifth exclusion (376) 0 feet, second exclusion (376) of the 4 feet, the fourth exclusion (377) 2,3 feet, fifty-fourth resistor (370) and the upper end of Next, the first rectifier bridge (449) 4-foot, nineteenth capacitor (405) is negative, the twentieth Capacitor (406) is negative, the first three terminal regulator (446) 3 feet, a fifth resistor (321) The right end of the sixth resistor (332) to the right end of the tenth manifold (445) of a foot, tenth Five resistors (331) of the lower end of the eighth capacitor (394) of the lower end of the tenth resistor (335) The lower end, the ninth capacitor (395) the lower end, the tenth capacitor (396) the lower end of the twenty- Nine resistors (345) of the lower end of the thirty-resistance (346) of the lower end of the thirty-second resistor (348) The lower end of the thirty-third resistor (349) of the lower end of the fifth semi-variable resistor (384) of the next End, the sixth semi-variable resistor (385) of the lower end of the seventh semi-variable resistor (386) of the lower end, Seven pin plug (4) of the pin 44, the sixth exclusion (379) 0 feet, four-pin plug (1) 14 feet, the twenty-fourth resistor (34) the lower end of 0, the twenty-fifth resistor (341) of the next End, the twenty-sixth resistor (342) of the lower end of the fifth capacitor (393) a negative electrode, a first half Variable resistor (380) of the lower end of the third capacitor (389) a negative electrode, a fourth capacitor (390) A negative electrode, a first Zener diode (432) of the cathode, a first capacitor (397) is negative, First thyristor (447) negative, forty-sixth resistor (362) of the lower end of the twelfth power Yung (398) negative, the thirteenth capacitor (399) of the lower end of the seventh manifold (442) 8 feet, eighth manifold (443) 8-pin, the first manifold (436) 3,5 feet, The first light-emitting diode (448) is negative, four-pin plug (2) of the pin 24, the fourteenth Capacitor (400) a negative electrode, the fifteenth capacitor (401) of the negative electrode, the sixteenth capacitor (403) Negative, fifty-seventh resistor (373) of the lower end of the eighteenth capacitor (404) and the negative Next, four-pin plug (1) of the pin 11, a first resistor (317) of the left end of the twenty-second Resistor (338) connected to the upper end and the four-pin plug (1) of the pin 12, a second resistor (318) The left end of the twenty-first resistor (337) and connected to the upper end of the four-pin plug (1) of the pin 13, a third resistor (319) of the left end of the twenty-resistor (336) and connected to the upper end of the first Resistor (317) of the right side, a fourth resistor (320) of the left end, a fifth resistor (321) of Left, a first diode (407) and connected to a positive electrode, a second resistor (318) the right end of the first Six resistors (322) of the left end of the seventh resistor (323) and the left end of the second diode (408) The positive and then, a third resistor (319) of the right side, the eleventh resistor (327) of the right side, Twelfth resistor (328) of the upper end of the seventh diode (413) connected to the positive and the fourth power Resistance (320) the right end of the eighth resistor (324) of the lower end, a first manifold (436) of 2 feet and then, the seventh resistor (324) of the right side, the ninth resistor (325) of the upper end of the first Manifold (436) 6 feet and then, the eleventh resistor (327) left end of the tenth resistor (326) The lower end of the first manifold (436) of 13 feet and then, the third diode (409) are Pole, eighth resistor (324) of the upper end of the first manifold (436) of a foot and then the fourth Diode (410) of the positive electrode, the ninth resistor (325) of the lower end, a first manifold (436) 7 feet and then, the first diode (407), a negative electrode, a third diode (409) is negative, Fifteenth resistor (331), a second manifold (437) to five feet and then, a second diode (408) A negative electrode, a fourth diode (410) is negative, thirty-third resistor (349) and the upper end of Fifth Manifold (440) 5 feet and then, sixth diode (412) is positive, the tenth power Resistance (326) of the upper end of the first manifold (436) 14 feet and then, the sixth diode (412) A negative electrode, the seventh diode (413) the negative, thirty resistor (346) of the upper end of the first Five manifold (440) to three feet and then, the first manifold (436), the twenty-third resistor (339) The right end of the fourth capacitor (390) of the cathode, a first diode (432) the negative and then, Ninth manifold (444) 9-pin, a first capacitor (387) connected to the positive and the ninth integration Block (444) of 4 feet, the first capacitor (387) and connected to the negative terminal of the ninth manifold (444) 6 feet, and the second capacitor (388) and connected to the left end of the ninth manifold (444) 8-pin, A second diode (408) connected to a negative electrode and a second diode (411) of the cathode, a third electrical Yung (389) of the cathode, the twenty-third resistor (339) to the left and then, twenty-second resistor (338) the lower end, the twenty-sixth resistor (342) of the upper end of the thirteenth diode (419) Positive and then, twenty-first resistor (337) of the lower end of the twenty-fifth resistor (341) of the Upper end of the fourteenth diode (420) connected to the positive and the twenty-resistance (336) of the lower end, Twenty-fourth resistor (340) of the upper end of the fifteenth diode (421) and connect the positive first Thirteen diode (419) is negative, the seventh capacitor (393) is positive, the fourth manifold (439) 2 feet and then, the fourteenth diode (420) is negative, the sixth capacitor (392) is positive, Fourth manifold (439) 4 feet and then, fifteenth diode (421) is negative, the fifth Capacitors (391) of the cathode, the fourth manifold (439) 6 feet and then, fourth manifold (439) 14 feet, sixteenth diode (422) and then the negative fourth manifold (439) to 13 feet, seventeenth diode (423) and then the negative fourth manifold (439) 12 Feet, eighteenth diode (424) and then the negative fourth manifold (439) 8,9, 10 feet, the first semi-variable resistor (380) of the sliding arm, and then, the sixteenth diode (424) The positive electrode, the twenty-seventh resistor (343) connected to the upper end and a second manifold (437) 1, 2,13,14 feet, a third manifold (438) of 3,5,10 feet, eighteenth capacitor (404) A positive electrode, a second semi-variable resistor (381) of the upper end, the third semi-variable resistor (382) of The upper end of the fourth semi-variable resistor (383) of the upper end of the eighteenth capacitor (404) and the positive Next, a second manifold (437) of 3,12 feet, the tenth resistor (334) of the lower end of the tenth Nine resistor (335) connected to an upper end and a second manifold (437) is 6 feet and twelfth resistor (329) the lower end of the fourteenth resistor (303) connected to the upper end and a second manifold (437) 7 feet, eighth diode (414) connected to the negative and the second manifold (437) 8-pin, Four-pin plug (4) of the pin 41, the second manifold (437) 9-foot, thirty-sixth power Resistance (352) of the left, the sixth exclusion (379) 2 feet and then, the second manifold (437) 10-pin, the eighth diode (414) of the cathode, the eighth capacitor (394) of the cathode, the first Sixteen resistor (332) and connected to the lower end of the seventeenth resistor (333) of the lower end of the eighteenth power Resistor (334) connected to the upper end and the ninth diode (415) of the cathode, the twenty-seventh resistor (353) The left, the fifth semi-variable resistor (384) of the upper end of the fifth semi-variable resistor (384) of Sliding arm, and then the fifth exclusion (378) of a foot, twenty-second diode (429) is positive, Tenth diode (416) of the cathode, the thirty-seventh resistor (353) to the left, the sixth half can Variable resistor (385) of the upper end of the sixth semi-variable resistor (385) of the sliding arm, fifth exclusion (378) 2 feet, twenty-fourth diode (430) is positive, eleventh diode (417), p. Forty-two resistor (358) of the left end of the seventh semi-variable resistor (386) of the upper end of the seventh half Variable resistor (386) of the sliding arm, fifth exclusion (378) 3-foot, twenty-fifth diode (431) connected to the positive and the third manifold (438) of a foot, thirty-sixth resistor (352) The right end of the thirty-ninth resistor (355) connected to the upper end and the third manifold (438) 7 Feet, forty-resistance (356) of the lower end of the forty-first resistor (357) connected to the upper end and, The third manifold (438) 8-foot, forty-third resistor (359) of the lower end of the forty-fourth Resistor (360) connected to the upper end and the third manifold (438) 9-foot, forty-third resistor (359) of the upper end of the forty-second resistor (358) and connected to the right end of the third manifold (438) 12 feet, forty-eighth resistor (365) of the lower end of the twelfth diode (418) Positive Pole, eleventh capacitor (397) connected to the positive and the third manifold (438) to 13 feet, Ninth diode (415) of the negative electrode, the tenth diode (416) is negative, the eleventh diode Tube (417) is negative and then, two pin plug (6) of the pin 61, the forty-fifth resistor (361) The upper and then, forty-fifth resistor (361) of the lower end of the forty-sixth resistor (362) of the Upper end of the first thyristor (447) trigger pole and then, the first SCR (447) of the cathode, Forty-seventh resistor (363) of the lower end of the forty-eighth resistor (364) of the upper end of the twelfth Diode (418) and then the negative fifth manifold (440) 9 feet, the sixth exclusion (379) 1 foot, forty-fourth resistor (360) connected to the lower end and the fifth manifold (440) to 13 feet, the sixth exclusion (379) 4-foot, forty-first resistor (357) and the lower end of Next, the fifth manifold (440) of a foot, nineteenth diode (425) is negative and then, Fifth Manifold (440) 6-foot, thirty-first resistor (347) of the lower end of the thirty-second Resistor (348) connected to the upper end and the fifth manifold (440) 7-foot, twenty-diode (426) the negative and then, twenty-diode (426) of the cathode, the tenth capacitor (396) The upper end of the fifth manifold (440) to 10 feet and then, the fifth manifold (440) 12 Feet, nineteenth diode (425) of the positive electrode, the ninth capacitor (395) of the cathode, the thirty- Four resistor (350) connected to the lower end and the fifth manifold (440) 8-foot, seven-pin plug (4) The pin 43 and connected, the fifth manifold (440) 14-foot, seven-pin plug (4) of the pin 42 and then, tenth manifold (445) 2,6 feet twelfth capacitor (398) is positive, Forty-ninth resistor (365) connected to the lower end and the tenth manifold (445) 5-foot, tenth Two capacitor (399) connected to the upper end and the tenth manifold (445) to 3 feet, the first exclusion (375) 4 feet and then, the seventh manifold (442) of a foot, the first exclusion (375) 0 feet and Next, the seventh manifold (442) 2 feet, the first exclusion (375) of one foot and then the seventh Manifold (442) 3 feet, the first exclusion (375) 3 feet, the first exclusion (375) 2 feet and then, the seventh manifold (442) 4-foot, first-exclusion (375) and 3 feet Next, the seventh manifold (442) of 5,6,10,13 feet, fifth exclusion (378) 4 Feet and then, seventh manifold (442) 7-foot, fourth exclusion (377) 0,1 feet and Next, the seventh Manifold (442) of 11 and 12 feet, the first relay (450) the lower end, Twenty-first diode (427) connected to the positive and the seventh manifold (442) to 15,16 Foot, twenty-diode (428) is negative, the fifty-fourth resistor (370) of the lower end of the first Fifty-five resistor (371) and connected to the upper end, two pin plug (3) of the pin 31, the first following the Power (450) of the movable contact and then, four-pin plug (2) of the pin 21, the twenty-sixth two Diode (463) and then the positive, four-pin plug (2) of the pin 22, the twenty-seventh two-pole Tube (464) and then the positive, four-pin plug (2) of the pin 23, the twenty-eighth diode (465) positive and then, twenty-sixth diode (463) is negative, the fiftieth resistance (366) The left end, the fourteenth capacitor (400) and then the positive, 27 and diode (464) of Negative, fifty-first resistor (367) left end of the fifteenth capacitor (401) connected to the positive and, Twenty-eighth diode (465) is negative, the fifty-second resistor (368) to the right end of the tenth Six capacitor (402) connected to the positive and, fiftieth resistor (366) to the right end of the second Zener Diode (433) the negative and then, fifty resistor (367) of the right side, a third Zener Diode (434) is negative and then, fifty second resistor (368) to the right end of the fourth Zener Diode (435) and then the negative, the eighth manifold (443) of a foot, the second regulator diode Tube (433) connected to the positive and the eighth manifold (443) to 2 feet, and the third Zener diode (434) connected to the positive and the eighth manifold (443) to 3 feet, the fourth Zener diode (435) Positive and connected, the eighth manifold (443) of 4,16 feet, second exclusion (376) 0 Feet and then, eighth manifold (443) of 5,15 feet, second exclusion (376) of a foot and Next, the eighth manifold (443) of 6,14 feet, second exclusion (376) 2 feet and then, Eighth manifold (443) of 11, 12 feet, the fourth exclusion (377) 4 feet and then, Twenty-seventh resistor (343) of the lower end of the fifth resistor (371) of the lower end of the fiftieth Six resistors (372) of the left, the seventeenth capacitor (403) of the cathode, the twenty-second diode (428) connected to the positive and the sixth manifold (441) of a foot, fifty-eighth resistor (374) The upper end of the second semi-variable resistor (381) of the lower end of the third semi-variable resistor (382) of Lower end of the fourth semi-variable resistor (383) connected to the lower end and the sixth manifold (441) 2 Feet, fifty-eighth resistor (374) of the lower end of the fifty-sixth resistor (372) connected to the right end and, Sixth manifold (441) to 3 feet, the second exclusion (376) 3-foot, fifty-seventh resistor (373) and connected to the upper end of the sixth manifold (441) 5-feet, the second semi-variable resistor (381) The sliding arm, and then the sixth manifold (441) 6,7 feet, twenty-fifth diode (431) Negative and then, sixth Manifold 441 8,9 feet, twenty-fourth diode 430 is negative Pole and then, sixth manifold (441), 10 feet, and the third semi-variable resistor (382) slip Arm, and then the sixth manifold (441) to 12 feet, the fourth semi-variable resistor (383) slip Arm, and then, Sixth Manifold (441) of 13-14 feet, twenty-diode (429) is negative and then, Phase shift module driver board (201) circuit connection, seven-pin plug (7) of the pin 77, the first A pulse transformer (469) of a foot, the second pulse transformer (470) of a foot, and the third Pulse transformer (471) of one foot and then, seven core socket head (7) of the pin 76, the fourth Transistor (466) c-foot, fifth transistor (467) c-foot, sixth transistor (468) And then c-foot, seven-pin plug (7) of the pin 74, the first transistor (463) of the e feet, A second resistor (452) of the right side, the second transistor e feet, a fourth resistor (454) to the right End of the third transistor (465) of the e feet, sixth resistor (456) and connected to the right end of the seven core Plug (7) of the pin 73, the fourth transistor (466) and connected with b foot, seven-pin plug (7) The pin 72, the fifth transistor (467) and connected with b foot, seven-pin plug (7) of the pin 71, the sixth transistor (468) b-foot and then the fourth transistor (466) feet of e, the first Fifty-nine Resistance (451) left end of the sixtieth resistor (454) and connected to the left end of the fifth three Diode (467) of the e feet, the sixty-first resistor (453) left end of the sixty-second resistor (454) The left and then the sixth transistor (468) of the e feet, the sixty-third resistor (455) to the left End of the sixty-fourth resistor (456) connected to the left end and the first transistor (463) b-foot, A first resistor (451) connected to the right end and a second transistor (464) b-pole, sixty-first Resistor (453) connected to the right end and the third transistor (465) b-pole, sixty-third resistor (455) and connected to the right end of the first transistor (463) c-pole, the first pulse transformer (469) 2 feet and then, a second transistor (464) c-pole, a second pulse transformer (470) of the 2 feet and then, a third transistor (465) c-pole, third pulse transformer (471) 2 Feet and then, the first pulse transformer (469) 3-foot, four-pin plug (8) of the pin 84 And then, the first pulse transformer (469) 4-foot, fifth Zener diode (457) Positive Pole and connected, the fifth Zener diode (457) is negative, four-pin plug (8) of the pin 83 And then, the first pulse transformer (469) 5-foot, four-pin plug (8) of the pin 82 feet And then, the first pulse transformer (469) 6-foot, sixth Zener diode (458) Positive Pole and then, sixth Zener diode (458) is negative, four-pin plug (8) of the pin 81 Feet and then, a second pulse transformer (470) 3-foot, four-pin plug (9) of the pin 94 And then, a second pulse transformer (470) 4-foot, seventh Zener diode (459) Positive Pole and connected, the seventh regulator (459) negative, four-pin plug (9) of the pin 93 and then, The second pulse transformer (470) 5-foot, four-pin plug (9) of the pin 92 and then, the first Two pulse transformer (470) 6-foot, eighth Zener diode (460) is positive and then, Eighth Zener diode (460) is negative, four-pin plug (9) of the pin 91 and then, the first Three pulse transformer (471) 3-foot, four-pin plug (10) of the pin 104 and then, the first Three pulse transformer (471) 4-foot ninth Zener diode (461) and then the positive, Ninth Zener diode (461) is negative, four-pin plug (10) of the pin 104 and then, The third pulse transformer (471) 5-foot, four-pin plug (10) of the pin 102 and then, The third pulse transformer (471) 6-foot, tenth Zener diode (462) is positive and then, Tenth Zener diode (462) is negative, four-pin plug (10) of the pin 101 and then, Phase drive plate (201) and control means (200) between the circuit connection, seven-pin connector (7) The pins 71,72,73,74,75,76,77 with seven through two core socket connection Wire with seven-pin plug (4) connecting the pin 41,42,43,44,45,46,47 patch Next, phase drive plate (201) and the thyristor SCR module (205) between the circuit connection, The first silicon tube (172) by connecting line four-pin socket (8 ') of the insert sleeve 81', 82 ', 83', 84 'and the four-pin plug (8) of the plug connector pins 81,82,83,84 And phase shift driver board (201) plug connection and the second thyristors (173) by connecting online The four-pin socket (9 ') of the insert sleeve 91', 92 ', 93', 94 'and the four-pin plug (9) of the The plug connector with pins 91,92,93,94 phase drive plate (201) plug connection, the first Three thyristors (174) by connecting line four-pin socket (10 ') of the insert sleeve 101', 102 ', 103', 104 'and the four-pin plug (10) of the pin 101,102,103,104 Plug connector with phase drive plate (201) plug connector, the control means (200) and the control board Power supply (202) connected between the circuit, the power supply control board (202) by connecting Line two-pin socket (5 ') of the insert sleeve 51', 52 'and two-pin plug (5) of the pin 51, The plug connector 52 to the control device (200) plug connector, the control means (200) and the lack of No. I believe the source (203) connected between the circuit, lack-phase signal source (203) through a connecting line The four-pin socket (2 ') of the insert sleeve 21', 22 ', 23', 24 'and the four-pin plug (2) of the The socket connector pins 21,22,23,24 and control means (200) plug connector, control Means (200) and the operating state display means (204) connected between the circuit, working status Display device (204) by connecting the line two-pin socket (3 ') of the insert sleeve 31', 32 ' With two pin plug (3) of the pins 31 and 32 connected to the control unit plug (200) patch Connection control means (200) and the thyristor SCR module (205) between the circuit connection, Thyristor SCR module (205) by connecting the line two-pin socket (6 ') of the insert sleeve 61', 62 'with two pin plug (6) of the pins 61 and 62 of the plug connector and control device (200) Plug connectors, four-pin socket (1 ') and the control device (200), four-prong plug (1) plug Connection. ...Embodiment 10

Emulsible concentrate (EC)

Also can prepare the cream preparation that contains the Chenopodium plant extracts.The Chenopodium plant extracts, emulsifier spreading agent/sticker and the carrier that contain 10-25% in the concentrate.The non-limiting example of preparation of the present invention is listed in the table 13.The embodiment of table 13:EC preparation

Composition	Content (%)
							The Chenopodium plant extracts	?25	?25	?25	?25	?25	?2
Rhodopex?CO-436	?5	?2.5	?-	?-	?1.25	?-
							Rhodopex?CO-433	?-	?-	?-	?-	?-	?-
Igepal?CO-430	?-	?2.5	?-	?-	?1.25	?2
							Igepal?CA-630	?-	?-	?5	?2.5	?-	?-
Igepal?CO-887	?-	?-	?-	?2.5	?-	?-
							Isopropyl alcohol	?-	?-	?10	?-	?-	?-
IsoparM	?-	?-	?60	?70	?-	?-
							Macol?NP95	?-	?-	?-	??-	?-	?2
THFA	?70	?70	?-	??-	?72.5	?7

Embodiment 11

The toxicity of RTU preparation

Be the toxicity of test preferred formulation, 30 female one-tenth mite back ofs the body be placed on the two-sided tape that this adhesive tape is bonded at (designer is ominous, 1968) in the 9cm culture dish down with the camel hairbrush.Three culture dishes of each concentration of each test formulation or product and contrast (for example water) preparation, each is handled day each processing and uses 90 mites altogether.

Use Gilson Pipetman ^TMP-1000 adds to each preparation and each 1ml of microfiltration water in contrast in the holder of Potter spray tower nozzle, and this spray tower is installed on the pallet and is connected with the manometer that is set to 3P.S.I..Before the test and the culture dish of after each is handled, weighing immediately to calculate the oil mass (mg/cm of each specimen deposition ²).

Now mix ready-to-use formulation according to the said method test and (hereinafter be called " RTU " preparation; Use kind 2 and DSD method), at variable concentrations (0.125,0.25,0.5,0.75 and 1%) under relatively exist on this RTU preparation and the market other kill mite product (synthetic) with natural thus relative effect determine to reach the required least concentration of lethality rate (＞95%) of expectation.

Entire method repeats 3 times, and each is handled and tests 270 mites altogether.

The lethality rate of 24 and 48 hours assessment mites after processing.If without any reaction, i.e. the not motion of leg, beak or belly thinks that then mite is dead to the exploration of thin camel hairbrush.For obtaining LC ₅₀(lethal concentration is with mg/cm for value ²Expression, it is the quantity of killing 50% test organisms required product; So LC ₅₀Be worth lowly more, the toxicity of product is big more), use POLO computer program (LeOra Software, 1987) that the count results after 48 hours is carried out probability analysis.Record lethality and the corresponding dosage (mg/cm that weighs ²), consider the changeability under each application layer.

The results are shown in Table 14 for these biologicall test gained.

Although the toxicity test of the present invention's record adopts female mite to carry out, those of ordinary skills know that these results show, because known male mite is littler than female mite, if use the observable lethality of male mite not higher, also can be identical.Table 14: the lethality of using the spider mite adult (T.urticae Koch) of chenopodium ambrosiodies RTU preparation and natural and the biological test test acquisition that carry out commercial preparation synthetic pesticide

Handle	Working concentration (%)	n	Lethality after 48 hours (%)	LC 50mg/cm 2(99% confidence interval)
					Contrast	0	1079	5
					RTU	0.5	270	100
RTU	0.75	270	100
					RTU	1.0	811	100
RTU		1980		0.00911
Dicofol	0.1	268	16
					Dicofol	0.25	181	4
Dicofol	0.5	179	10
The Wilson Dormant oils	1.0 *	181	10
Safer	1.0 *	272	99

^*Recommended dose on the label

Also the insect to several serious harm cultivated plants has carried out above-mentioned test.Test approaches is greenhouse whitefly, alfalfa thrips, black peach aphid and Bemisia argentifolii.

The result of table 15 record shows that the RTU product all has toxicity to all test organisms.Calculate the LC of Trialeurodes vaporariorum Westwood and black peach aphid ₅₀, and result (LC ₅₀Be respectively 0.00131mg/cm ²And 0.0009mg/cm ²) show that this product can obtain identical or more effective activity to these insects with the spider mite is compared.Table 15: the lethality (%) that the existing mixed existing tracking biologicall test of the spider mite being carried out with (RTU) preparation of use chenopodium ambrosiodies is tested

Handle	Working concentration	Lethality after 48 hours (%)
									The greenhouse whitefly adult	The alfalfa thrips adult	The black peach aphid adult	The Bemisia argentifolii nymph
The water contrast	0	6	3.8	11	0.2
RTU	0.0625	63	-	-	-
								0.125	76	85.4	58	-
	0.25	84	100	58	-
								0.5	97	100	86	93
	1.0	-	-	-	98
									0.00131	*	.0009	*	LC 50(mg/cm 2)

^*Data deficiencies is carried out LC ₅₀Analyze Embodiment 12

The toxicity of EC preparation

Missible oil (EC) preparation that adopts the method identical with the RTU preparation to use embodiment 10 to describe experimentizes to spider mite adult.Listed result shows that in concentration be at 1.0% o'clock in the table 16, and EC preparation and Safer Soap are effective equally, and have higher genotoxic potential, because the LC of EC product ₅₀(0.009) lower than Safer Soap (0.016).Table 16: that uses that chenopodium ambrosiodies cream preparation and commercially available natural Pesticidal products obtain becomes mite lethality (%)

Handle	Working concentration (%)	Testing time	Lethality after 48 hours (%)	CL 50mg/cm 2
The water contrast	0	546	6
EC	0.125	180	32
					EC	0.25	271	53
EC	0.5	270	75
					EC	1.0	272	96
				0.009(99％ conf.Int)
Safer?Soap	1.0		97
									0.016(90％ conf.Int)

Embodiment 13

Miticide composition of the present invention is to the effect of ovum and the nymph of spider mite

Also use the RTU preparation that ovum and nymph spider mite are tested.Result of the test (table 17) in the ovum phase shows the RTU preparation under 0.5% oil solution condition, and ovum is produced certain effect, obtains 30% lethality.Expect that higher oil concentration will obtain higher ovum effect extremely.Table 17: use the RTU preparation of American wormseed oil, the egg mortality of spider mite (T.urticae Koch) that obtain

Handle	Working concentration (%)	Testing time (n)	Lethality (%)
				Contrast	0	196	7.70
				RTU	0.125	201	6.50
RTU	0.25	200	11.0
				RTU	0.5	188	30.3

Similarly the RTU preparation is to the effect of nymph, even under 0.5% concentration conditions, RTU obtains better effect (95.8%) (table 18) than existing Olivomitecidin (80.1%) or Safer (61.7%).Table 18: the lethality that the commercial preparation obtained of using RTU Chenopodium plant extracts preparation and synthetic and natural prodcuts to spider mite nymph (T.urticae Koch)

Handle	Working concentration (%)	Testing time (n)	Lethality (%)
				Contrast	0	256	7.6
				RTU	0.0625	76	77.2
RTU	0.125	208	45
				RTU	0.25	238	71
RTU	0.5	120	95.8
Olivomitecidin	1 1	303	80.1
				Safer	1 1	293	61.7

Recommended dose Embodiment 14

The residual activity of RTU preparation of the present invention and with the comparison of commercially available miticide product

Also having tested the RTU preparation and compared to the residual activity of spider mite and with the natural and sintetics that has put goods on the market, (is kelthane ^TM, neat pier is full plain ^TM, Safer ' s ^TMSoap and Wilson ' s Dormant oils).This test method comprises that preparation contains the bottle of nutrient solution, puts into broadbean leaf in each bottle.18 leaves of each test concentrations preparation adopt normal concentration to spray each blade until tumbling (pesticide solution tumbles from the blade face) and drying.Spray placed 10 spider mites on 9 blades in back 1 hour, and after handling one day 10 spider mites was placed in addition on 9 blades.Observe lethality after 24 and 48 hours after being placed on mite on the blade.All test operations repeat 3 times.

When processing was placed on mite on the plant after 1 hour, the results are shown in Table 19 for the residual activity of different product.These results show that RTU has residual activity, and its effect is higher than the Safer product.Yet, be lower than the residual activity of chemosynthesis product such as kelthane and Olivomitecidin.Table 19: handle broadbean leaf after 1 hour, the spider mite is contacted under the situation with product, the synthetic and natural acaricidal residual activity of RTU preparation and selection

Handle	Concentration (%)	Testing time (n)	Place 48 hours lethality rate of acarid at back 1 hour plant surface of processing
				Contrast	0	150	5.0
				RTU	1	90	23.1
Kelthane	0.10	60	45.6
				Olivomitecidin	0.03	90	95.0
Safer	1	60	4.2

The above results shows that the RTU preparation has very low lasting effect (when with product treatment after 1 hour, when putting into insect on plant, the lethality of spider mite about 23%) in environment.Therefore the RTU preparation satisfies and does not injure the requirement that the natural enemy population prevents and treats the integrated pest management plan of method. Embodiment 15

Extract is to the confirmation of other mite class acaricidal activity

For confirming that preparation of the present invention to the common effect that infects the plant mites class, infects the mite class of plant to another kind, the golden pawl mite that promptly shows with the very near taxonomic relation of T.urticae Koch has carried out specific biologicall test test.

With the RTU preparation spider mite adult of the gold of the insect in apple orchard pawl mite is tagged effect test to confirm that it is as acaricidal broad spectrum of activity according to above-mentioned same testing program.The result has confirmed that the acaricidal effect of chenopodium ambrosioides extract tagging property of conduct not only is confined to the activity (table 20) to T.urticae Koch.Table 20: the golden pawl mite lethality of using the RTU preparation to obtain

Handle	Concentration (%)	Test quantity (n)	Lethality rate (%)
				Contrast	0	280	28.8
				RTU	0.0625	291	93.6
RTU	0.125	14	100

Embodiment 16

The adaptability of integrated pest management plan:

Product by extract development of the present invention does not have toxicity to mammal

And unlikely it is produced resistance

The present composition is the tagging property miticide of low-residual activity.The performance of these compositions is the low lasting effect in environment (with after this product treatment 1 hour, insect is placed on the plant, the lethality of spider mite is 22%).Therefore these compositions and integrated pest management plan (IPM) are by " ecological selectivity " (van den Bosch and Stem, the application of biological control effect 1962) is compatible, if promptly in the test place, after initial the using, beneficial organism can be survived and then be had selectivity.Nicotine is a kind of compound of finding in plant, and known very early have very low or do not have residual activity (Pipper 1956 beneficial organism; Walton and Whitehead1944).

And the complexity that the plant resource product is formed promptly contains more than one active component such as those products of the present invention, also makes unlikely to produce resistance.IPM encourages to use ' biological agricultural chemicals rationally ' (being also referred to as hypotoxicity or biological insecticides).Biological agricultural chemicals rationally is their selectivity to target pest than the superior part of traditional chemical pesticide, the shorter interval that useful insect and peasant or greenhouse staff's lower toxicity and workman got back to the field.

Plants essential oil is the mixture of the complexity of many multiple compounds with anti-insect and mite pest activity, and multiple compound plays a role separately or produces synergistic effect mutually, thereby by the contact repellent or kill off the insect pests.Because the complexity of this mixture, observed and compared insect with synthetic pesticide these products are not easy to generate resistance.A few miticide is put on market, but regrettably wherein many products has been produced resistance.

Really confirmed the repeated treatments (active component in neem kernel extract) of pure nimbin, compared with the black peach aphid control group that is untreated and in 40 generations, produce 9 times resistance (Feng andIsman, 1995) black peach aphid; Yet in the above-mentioned identical time limit (40 generation), the use repeatedly of full neem extract does not produce tangible resistance.This shows that the present composition compares with the chemosynthesis miticide of present use that to have significant advantage be that it can use for a long time.

Although the present invention is above disclosing use Chenopodium plant extracts, the present invention is not limited to this.In fact, those of ordinary skills can select other type plant, as long as contain acaricidal activity material (as Central Asia Artemisia santonica and the chrysanthemum wormwood artemisia that hereinafter exemplifies) in the plant. Embodiment 17

Other plant extracts with acaricidal activity

Autumn in 1993 full-bloom stage, cultivation area in agricultural and agricultural food product Canada experimental plot (Agricultureand Agri-Food Canada experimental farm), L ' Acadie (45 18 ' N, 7320 ' W, the Quebec, Canada, the whole strain plant of gather Central Asia Artemisia santonica and chrysanthemum wormwood artemisia.Microwave householder method (Microwave Assisted Process) (MAP ^TM) and two kinds of variants of steam distillation method be water distillation (DW) and direct steam distillation (DSD) (Duerbeck, K., 1993), be used to extract fresh vegetable material.

The MAP method uses microwave to excite hydrone in the plant tissue and cause plant cell to break and to discharge the essential oil (Belanger et al., 1991) that plant cell is held back in organizing outward.For carrying out this method, will put in order strain plant each several part and carry out fragmentation and 20g is immersed in the 100ml hexane, then with 675W intensity with 2450Mhz irradiation 90 seconds.

Water distillation (DW) and DSD carry out according to said method.In brief, use the vegetable material of capacity as the about 20kg of distiller processing of 380L.In the DW method, vegetable material is completely infused in the water of proper volume, adopt the suprabasil steam pipe coil that is fixed on the distillation body to be heated to boiling then.

In the DSD method, vegetable material is carried on distillation body inside also evenly and loosely loads so that water vapour can pass through vegetable material unobstructedly.Water vapour oozes out by the external generator generation and by pot bottom and passes through vegetable material.The feeding rate setting of water vapour is (300ml/min).According to these two kinds of methods, from vegetable material, discharge oil ingredient, and itself and water vapour are cooled off in condenser to be divided into two kinds of components, You Heshui.

Analyze essential oil and extract by capillary gas chromatography (GC).The Varian 6000 series Vista that two flame ionization detectors are equipped with in use carry out GC.Calculate peak area by Varian DS 654 integrators.Use the fused silica column of SPB-1 (30m X 0.25mm , 0.25 μ m) and Supelcowax (30m, 0.25mm , 0.25 μ m), helium is as carrier gas, and flow velocity is 30cm/sec (1.5ml/min).Set furnace temperature with the speed of 2/min by 40 rise to 240 and syringe and detector temperature be set at 230 and 250 respectively.

Each floristics adopts following method respectively.

With every kind of extract is that 3 concentration of MAP, DW and DSD extract are tested on the spider mite.The preparation of emulsion is at first to prepare 300ml emulsifier storing solution (0.32% AlkamulEL-620), industrial alcohol (9%) and microfiltration water.Microfiltration water is with comparing.With 40,80,160 and 320 μ l oil stand-by storage liquid complements to 4ml and obtains 1,2,4 and 8% solution respectively.Do not prepare the Central Asia Artemisia santonica of 8% concentration and the MAP extract of chrysanthemum wormwood artemisia, because these oily lazy weights.

With the camel hairbrush 30 female one-tenth mites back ofs the body are placed on the two-sided tape down, this adhesive tape be bonded in the 9cm culture dish (designer is ominous, 1968, as mentioned).Three culture dishes of each prepared at concentrations and the control group of the oil of three kinds of method extractions are water, and each is handled day every kind of extracting method and uses 90 mites altogether.

For carrying out each processing (processing of each culture dish), with every kind of preparation and each 1ml of microfiltration water Gilson Pipetman in contrast ^P-1000 adds in the holder of Potter spray tower nozzle, and the piezometer that is set to 3 PSI is installed on the top of the shelf and be connected with to this spray tower.Before the test and the culture dish and on average deposit 205mg (± 42 on each culture dish of after each is handled, weighing immediately; N=50) solution is represented each concentration 2.1 (1%), 4.1 (2%), 8.2 (4%) and 16.4mg/cm ²(8%) deposition oil.

Whole procedure is carried out twice (1 and 2% Central Asia Artemisia santonica MAP and 4% chrysanthemum wormwood artemisia MAP solution) and three times (all DW of remaining MAP and two plant species and DSD solution).Use the 3rd test of MAP extract not finish, because the lazy weight of oil.

Handle the lethality of back assessment in 24 and 48 hours mite.As previously mentioned, if mite to the exploration of thin camel hairbrush without any reaction, promptly do not have the motion of leg, beak or belly to think that then mite is dead.Use POLO computer program (LeOra Software, 1987) that count results after 48 hours is carried out probability analysis.Record and the corresponding dosage (mg/cm that weighs ²) lethality, consider the variation under each usage amount.LC ₅₀The significant difference of value be by POLO relatively (LeOraSoftware, 1987, as mentioned) 95% confidence interval of Ji Suaning is definite. The analysis of oil

The chromatography that the oil that extracts from Central Asia Artemisia santonica carries out shows the difference (table 19) that can cause between the extracting method on the chemical composition.In DSD oil, do not contain sabinene and α-Limonene Tong, and in MAP and DW oil, have two kinds of materials; And be accredited as C ₁₅H ₂₄Compound be present in the DSD oil, and do not exist in MAP and the DW oil.Table 21: relative amount (%) the compound K .1.a of every kind of extracting method compound that the main compound that contains in the Central Asia Artemisia santonica oil that adopts MAP, DW and DSD method to extract contains ¹K.1.p ²MAP DW DSD sabinene--- ³1,101 2.4 4.5 0 α ,-Limonene alcohol, 1,086 1,380 1.4 2.9 0 β ,-Limonene alcohol, 1,091 1,399 11.5 32.1 12.3C ₁₀H ₁₆O 1,113 1,439 54.1 36.1 49.1 unknown compounds 1,465 1,832 2.1 2.1 3.4#1C ₁₅H ₂₄1,485 1,868 00 4.2

¹The Kovats index of nonpolar DB-1 post

²The Kovats index of polarity S upelcowax post

³Unknown

In tanacetum parthenium extract, β-thujones are main component (MAP:92.2% of all three kinds of extracting method; DW:87.6%; DSD:91.9%) (table 22).Pure and mild α-the cubebene of terpene-4-is present in the DW extract, and does not exist in MAP and the DSD extract.Table 22: by MAP, relative amount (%) the compound K .1.a of every kind of extracting method compound that the main compound that contains in the chrysanthemum sagebrush oil that DW and DSD method are extracted contains ¹K.1.p ²The MAP DW DSD α-Limonene alcohol 1083.4 1,378 0.357 0.935 1.058 β-Limonene alcohol 1,094 1,400 92.203 87.635 91.875 Zhang Nao 1,118 1,622 1.064 0.963 0 Tie Pin-4-alcohol 1158.3 1,559 0 0.552 0 α-clear Qie You of Bi Xi 1,460 1,663 0 5.063 0

¹The Kovats index of nonpolar DB-1 post

²The Kovats index of polarity S upelcowax post

³Nonpolar DB-1 post The biological test result of the test

After 48 hours, three kinds of extracts of all of Central Asia Artemisia santonica (MAP, DW and DSD) all have cause death active (table 23) to T.urticae Koch.Yet, there are differences on the toxic level of each extract to T.urticae Koch.Therefore, when 4% concentration, the oil content that MAP and DW method are extracted does not obtain 52.7 and 51.1% lethality rate, and the oil that the DSD method is extracted obtains 83.2% lethality rate.The LC of the oil that the MAP method is extracted ₅₀Value (0.134mg/cm ²) and DW method (0.130mg/cm ²) and the LC of the oil that the DSD method is extracted ₅₀Be worth lower (0.043mg/cm ²) (table 24).Table 23: use MAP, 48 hours T.urticae Koches become the lethality of mite after the Central Asia Artemisia santonica oil processing that DW and DSD method are extracted

The concentration (%) of oil ¹Extracting method 0124 8MAP 5.3 15.7 ²19.5 ²52.7--- ³DW 2.8 20.5 28.2 51.1 65.6DSD 2.6 42.1 71.3 83.2 92.8

¹N=each concentration except as otherwise noted uses 270 mites

²N=180 mite

³The lazy weight of MAP oil is to carry out biologicall test test card 24 in this concentration: use MAP, after the Central Asia Artemisia santonica oil processing that DW and DSD method are extracted 48 hours, T.urticae Koch became the lethality probability analysis side of extraction n intercept ± gradient ± SEM ratio of mite ^*LC ₅₀(mg/c ²) LC ₅₀99% method SEM dislike MAP 900 2.12 ± 0.31 2.44 ± 0.30 8.06 0.134 0.096-0.280DW 1,260 1.72 ± 0.15 1.94 ± 0.16 11.78 0.130 0.081-0.205DSD 1,260 2.93 ± 0.18 2.15 ± 0.16 13.65 0.043 0.028-0.057 between Zhi Xin Qu

^*(t value＞1.96 item think under the P=0.01 level it is significant)

Tanacetum parthenium extract also has cause death active (table 25) to T.urticae Koch, and the extract that DW and DSD method obtain has higher acaricidal activity than the extract that MAP method obtains.When 4% concentration, the oil content that DW and DSD method are extracted does not obtain 60.4% and 75.6% lethality rate, and the oil that MAP extracts obtains 16.7% lethality rate.Table 25: use MA, the chrysanthemum sagebrush oil that DW and DSD method are extracted was handled back 48 hours, and T.urticae Koch becomes the lethality of mite

The concentration (%) of oil ¹Extracting method 0124 8MAP 6.3 17.8 11.1 16.7 ²--- ³DW 5.6 48.1 64.9 60.4 89.3DSD 5.0 52.9 64.1 75.6 95.6

¹N=is 270 mites of each concentration except as otherwise noted

²N=180 mite

³MAP ^TMThe lazy weight of oil relatively uses the probability analysis that the lethality data of acquisition are tested in test of giving birth to of DW and DSD method to carry out the biologicall test test in this concentration; Yet the analysis of MAP lethality data draws incredible result, because the high diversity (table 26) of lethality % value between each repeated experiments of handling under same concentrations.This species diversity is likely because the physical characteristic of MAP extract causes.In the method, organic compound such as wax and resin with essential oil by discharging in the plant cell.These products can not fully mix by the Alkamuls-EL620 emulsifier, cause forming inhomogeneous solution.Table 26: handled back 48 hours by the chrysanthemum sagebrush oil that DW and DSD method are extracted, T.urticae Koch becomes the lethality probability analysis side of extraction n intercept ± gradient ± SEM ratio of mite ^*LC ₅₀(mg/c ²) LC ₅₀99% method SEM dislike confidence interval DW 1,350 1.81 ± 0.15 1.42 ± 0.14 10.08 0.054 0.013-0.088DSD 350 2.50 ± 0.17 1.86 ± 0.15 12.43 0.046 0.022-0.066

^*(t value＞1.96 item think under the P=0.01 level it is significant) Discuss

Change although observed some in the biologicall test of Central Asia Artemisia santonica and the tanacetum parthenium extract test, the present invention shows that the Central Asia Artemisia santonica oil that other method of Central Asia Artemisia santonica oil ratio that the DSD method is extracted is extracted can more effectively prevent and treat the spider mite.4.2% the sequiterpene C that in DSD, contains ₁₅H ₂₄Compound, and in other two kinds of extracts, do not have (table 21), this may be that it has higher levels of bioactive reason.Yet, unknown C in the Artemisia santonica of the Central Asia ₁₅H ₂₄The evaluation of compound, and adopt the biologicall test of each compound of above-mentioned three kinds of extracting method to test for determining that the active component that exists in the Artemisia santonica oil of the Central Asia is necessary.

Between the oil of tansy that extracts by DW and DSD method is being similarly aspect the biologically active, this means that the contribution of acaricidal activity of the oil that 4-terpineol and α-cubebene (be present among the DW and do not have among the DSD) extract the DW method is very little.Owing to contain quite high β-thujone percentage compositions in all three kinds of extracts, this component may be main active component (being abbreviated as a.i.), and other chemical composition has negligible activity.This can explain the similar results that DW extract when 4% concentration (60.4% lethality rate and 87.6% β ,-thujones) and DSD extract (75.5% lethality rate and 91.88% β ,-thujones) obtain, but can not explain the low actual (16.7% lethality rate and 92.2% β ,-thujones) of MAP extract.Owing to have wax and a resin, the MAP extract may be not by fully emulsified in solution.

In the extract evaluation of active component for the development plant pesticide register the institute requisite.The variation of essential oil extract series on activity must minimize to obtain the stable product of toxicity.In addition, must consider other for the oil extract that obtains to have high bioactivity and change as phenology period of plant, gather in the crops the water content % of material and the plant part (as mentioned above) of selective extraction.DSD is the method for accepting extensively most for people that is used for the large-scale production essential oil, because under the Artemisia santonica situation of the Central Asia, can produce the more highly toxic oil, also should consider the large-scale production of biologically active oil, the DSD method compare with other extracting method need be lower expense and yield (Chiasson and Belanger, unpub result).Producing water vapour institute energy requirement among the DSD significantly is lower than the DW method and makes water boiling institute energy requirement.MAP is still at the experimental stage, can't be used for large-scale production. Conclusion

Therefore, the invention describes plant acaricide, oil extract and miticide composition, they show very high activity to two kinds of mites of infecting plant.These compositions have also shown acaricidal activity to the different phase of mite in life cycle.

In addition, these compositions 1 hour meter behind the blade of introduce handling reveals the spider mite is had medium residual activity, and they are suitable for the requirement of integrated pest management plan like this.In a word, miticide composition of the present invention is compared with known mite product extremely and is shown multiple advantage.

Shown in the preferred embodiment of the invention,, comprise terpene such as p-cymene, α-terpinene, limonene, thymol, carvacrol and ascaridole to the plant extracts of the present invention that the plant mites class has acaricidal activity.And extract of the present invention shows has the double activity that kills mite and desinsection.

Although the present invention is described by above-mentioned preferred embodiment, as appended claims limits, can also makes improvements and do not break away from spirit of the present invention and feature the present invention.

List of references Aellen，P.& T.Just.1943.Key and synopsis of the American species of the genus Chenopodium L. Amer. Midl.Nat.30：47-76. Ascher，K.R.S.and Cwilich，R.1960.Laboratory evaluation of acaricides against Tetranychus telarius L.on sugar beet and on beans. Israel J.agric. Res.10：159-163. Anonymous.1968.Method for the boll weevil and tentative method for spider mites.First Conference on Test Methods for Resistance in Insects of Agricultural Importance.Bull.Entomol.Soc.Am.14，31-37. Anonymous.1974.Recommended methods for the detecticn and measurement of resistance of agricultural pests to pesticides.10 tentative methods for spider mites and their eggs Tetranychus spp.and Panonychus ulmi Koc.PI.Prot.Bull.FAO.22：103-107. Bélanger A.，Landry B.，Dextraze L.，Bélanger J.M.R.，ParéJ.R.J.，1991， Extraction et détermination de composés volatiles de I’ail (Allium sativum)， Riv.Ital.EPPOS  2：455. Brielmann，H.L.1999.Phytochemicals：The Chemical Components of Plants..In：Natural Products from Plants. Kaufman，P.B.，L.J.Cseke，S. Warber，J.A.Duke and H.L.Brielmann，eds.CRC Press.Boca Raton.343 pp. Bumouf-Radosevich，M.and N.E.Delfel.1984.High-performance liquid chromatography of oleanane-type triterpenes.J.Chromatography 292：403- 409. Bumouf-Radosevich，M.et al.1985.Gas chromatography-mass spectrometry of oleanane- and ursane-type triterpenes - application to Chenopodium quinoa triterpenes.Phytochemistry 24：2063-2066. Busvine，J.R.1958.The detection and measurement of insecticide resistance.Proc.15 ^th International Congress of Zoology. Chandel，R.S.and R.P.Rastogi.1980.Triterpenoid saponins and sapogenins：1973-1978.Phytochemistry 19：1889-1908. Chialva，F.；Lidlle，P.A.P.；Doglia，G.1983.Chemotaxonomy of Wormwood (Artemisia Absinthium L.).A.Lebensm Unters Forsch 176：363-366. Coats，J.R.1994.Risks from Natural versus Synthetic Insecticides.Ann.Rev. Entomol.39：489-515. Dennehy，T.J.，J. Granett and T.F.Leigh.1983. Relevance of Slide-Dip and Residual Bioassay Comparisons to Detection of Resistnce in Spider Mites. J.Econ.Entomol.76：1225-1230. Dennehy，T.J.，A.W.Famham amd I.Denholm.1992.The Mircroimmersion bioassay：A Novel Method for the Topical Application of Pesticides to Spider Mites (Acarina：Tetramuchidae).Pesticide Science Dittrich，V.1962.A Comparative Study of Toxicological Test Methods on a Population of the Two-Spotted Spider Mite (Tetranychus telarius).J.Econ. Entomol.55：644-648. Duerbeck，K..1993.The Distillation of Essential Oils.Manufacturng and Plant Construction Handbook.Protrade：Dept.Of Foodstuffs and Agricultural Products.Eschbom，Germany.165 pp. Ebeling，W.and R.J.Pence.1953.Pesticide formulation：influence of formulation on effectivenes.J.Agr.Food Chem.1：386-397. El Gangaihi，S.E.；Amer，S.A.A.；Mohamed，S.M.，1996，Biological Activity of Thyme Oil and Thymol against Tetranychus urticae Koch.Anz.Schad. Pflanz.Umwel.69，157-159. Feng，R.and M.B.Isman.1995，Selection for resistance to azadirachtin in the green peach aphid，Myzus persicae，Experimentia 51：831-833. Flint，M.L.1990.Pests of the garden and small farm.A grower’s Guide to Using Less Pesticide.Statewide IPM Project.U.of Califomia.Publication 3332. Flint，M.L.，S.Daar and R.Molinar.1991.Establishing Integrated Pest Management Policies and Programs：A Guide for Public Agencies.U.of Califomia.Statewide IPM Project.UC IPM Publication 12.9pp. Foott，W.H.and H.R.Boyce.1966.A Modification of the Leaf-Disc Technique for Acaricide Tests.Proc.Entomol.Soc.Ont.96：117-119. Georghiou，G.P.1990.Overview of insecticide resistance.In ′Managing Resistance to Agrochemicals.M.E.Green，H.M.Le Baron and W.K.Moberg， eds.，ACS Symposium Series.Vol.421：18-41. Jackson，S.A.L and R.K.Hay.1994.Characteristics of varieties of thyme (Thymus vulgaris L.)for use in the UK：Oil content，composition and related characters.J.of Hort.Sci.69，：275-281. LeOra Sofware.POLO-PC，1987，A user’guide to probit or logit analysis. LeOra Software，Berkeley CA. Lippold，P.1963.Acaricidal testing techniques in the two-spotted spider mite. Adv.Acarol.1：174-180. Malik，M.M.and S.H.M.Naqvi.1984.Screening of some indigenous plants as repellents or antifeedants for stored grain insects.J.Stored Product Research 20(1)：41-44. Mansour，F.，K.R.S.Ascher et N.Omari.1986.Effect of neem seed kernel extracts from different solvents on the predacious mite Phytoseiulus persimilis and the phytophagous mite Tetranychus cinnabarinus as well as on the predatory spider Chiracanthium mildei.In Natural Pesticides from the Neem Tree(Azadirachta indica) and other tropical plants.H.Schmutterer， K.R.S.Ascher eds.Proc.Third Int.Neem Conf.，Nairobi，Kenya.July 1986； Pp.577-590. Meister，R.T.ed.1999.Farm Chemicals Handbook.Vol.85.Meister Publishing Company，Willoughby，Ohio. Perez-Souto，N.，R.J.Lynch，G.Measures，J.T.Hann.1992.Use of high- performance liquid chromatographic peak deconvolution and peak labelling to identify antiparasitic components in plant extracts.J.of Chrom.593：209- 215. Qasem，J.R.and T.A.Hill.1989.Possible rle of allelopathy in the competition between tomato.Senecio vulgaris and Chenopodium album. Weed Research 29：349-356. Quarles，W.1992.Botanical Pesticides from Chenopodium？The IPM Practitioner 14(2)：1-11. Ripper，W.E.1956.Effect of pesticides on balance of arthropod populations. Ann.Rev.Entomol.1：403-438. Risi，J.and N.W.Galwey.1984.The Chenopodium grains of the Andes：Inca crops for modem agriculture.Advances in Applied Biology 10：145-216. Roush，R.T.and J.A.McKenzie.1987.Ecological genetics of insecticide and acaricide resistance.Ann.Rev.Entomol.32：361-80 Sundaram，K.M.S.and L.Sloane.1995.Effects of Pure and Formulated Azadirachtin，a Neem-based Biopesticide，on the Phytophagous Spider Mite， Tetranychus urticae Koch.J.Environ.Sci.Health B30：801-814. Tumock，W.J.1985.Development，survival，and reproductive parameters of bertha armyworm，Mamestra configurata(Lepidoptera：Noctuidae)on four plant species.Canadian Entomologist 117：1267-1271. Van Den Bosch，R.and V.M.Stem.1962.The Integration of Chemical and Biological Control of Arthropod Pests.Ann.Rev.Entomol.7：367-386. Voroshilov[or Woroshilov]，V.N.1942.Revision des espéces de Chenopodium de la section Ambrina(Spach)Hook.fil.Bot.Zhum.27(3-4)： 33-47.[in Russian] Walker，L.T.，A.L Boswell and F.F.Smith.1973.Resistance of spider mites to acaricides comparison of slide dip and leaf dip methods.J.Econ.Entomol. 66：549-550. Walton，R.R.and F.E.Whitehead.1944.Effect of nicotine dust on the melon aphid and its natural enemies.J.Econ.Entomol.37：310-31.

Claims (20)

1. herbaceous plant extract, it has acaricidal activity to the mite class that infects plant.

2. according to the plant extracts of claim 1, wherein said extract derives from chenopodium ambrosiodies.

3. according to the plant extracts of claim 2, wherein said mite class is the mite of Tetranychus.

4. according to the extract of claim 3, wherein said mite class is a T.urticae Koch.

5. according to the extract of claim 1, also the insect of infecting plant had insecticidal activity.

6. one kind comprises the composition of plant extracts of claim 1 and suitable carrier, and wherein said plant extracts accounts for 0.125% to 10% of described composition.

7. the composition of claim 6, wherein said carrier is the emulsifier that is fit to.

8. the composition of claim 7, wherein said emulsifier is the blend of at least a non-anion emulsifier and at least a anion emulsifier.

9. the composition of claim 7, wherein said emulsifier is non-anion emulsifier.

10. the composition of claim 7, wherein said emulsifier is an anion emulsifier.

11. the composition of claim 7 comprises 0.125 to 5% emulsifier.

12., further contain a kind of spreading agent/sticker according to the composition of claim 6.

13. the miticide composition of the mite class of plant is infected in a control, comprise about α-terpinene of 5 to 60%, about p-cymene of 5 to 30%, about 1 to 20% De limonene, about ascaridole of 10 to 70%, about 3 to 20% different ascaridole, about thymol of 0.25 to 10% and about carvacrol of 0.25 to 10%.

14. according to the composition of claim 13, the mite that its described mite class is a Tetranychus.

15., further contain suitable emulsifier according to the composition of claim 13.

16. the method for the mite class of plant is infected in a control, this method comprises and is applied to the place that needs are prevented and treated with containing the Chenopodium plant extracts of mite effective dose extremely and the composition of suitable carrier.

17. the method for claim 16, the Chenopodium plant that wherein said Chenopodium plant is the chenopodium ambrosiodies kind.

18. the method for claim 17, wherein said carrier are the emulsifier that is fit to.

19. the method for claim 17, wherein said mite class are the mites of tetranychid kind.

20. the method for the mite class of plant is infected in a control, this method comprises and is applied to the place that needs are prevented and treated with containing the Chenopodium plant extracts of mite effective dose extremely and the described composition of claim 6 of suitable carrier.