CN101209062A - Compound plant source agricultural fungicide and preparation thereof - Google Patents
Compound plant source agricultural fungicide and preparation thereof Download PDFInfo
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- CN101209062A CN101209062A CNA2006101704854A CN200610170485A CN101209062A CN 101209062 A CN101209062 A CN 101209062A CN A2006101704854 A CNA2006101704854 A CN A2006101704854A CN 200610170485 A CN200610170485 A CN 200610170485A CN 101209062 A CN101209062 A CN 101209062A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a compound plant source disinfectant and a method for preparing the disinfectant. The disinfectant is made by the materials of cassia oil, sweetflag, sophora flavescens and eucalyptus oil of traditional Chinese medicines. The main effective components of the disinfectant are the cassia oil, the Beta-asarol and the matrine. After being extracted, the materials are matched with an accessory ingredient to be made into various missible oil, emulsion in water and other various preparations. The disinfectant has broad spectrum and excellent restraining effects on various plant pathogenicbacterias. The plant source disinfectant of the invention has the advantages of high efficiency, low mammalian toxicity; the harmful bacterias are difficult to generate drug fastness on the disinfectant. A field test shows that the control effect on the gen-seng leaf spot is better than that of 70 percent of mancozeb. The invention can be taken as an ideal and eco-friendly pesticide.
Description
One, technical field
The present invention relates to a kind of agricultural chemicals, particularly a kind of botanical fungicide.
Two, background technology
The applied chemistry synthetic pesticide, it has the characteristics that preventive effect is good, effect is fast, is one of important means of the extermination of disease and insect pest at present.But long-term, a large amount of, irrational use chemical synthetic pesticides, the application of especially high poison, persistent pesticide, cause and cause that residue of pesticide exceed standard, environment and agricultural product are contaminated, harm human and livestock health, damage by disease and insect develop immunity to drugs, preventive effect reduces even a large amount of killed natural enemies, cause that damage by disease and insect is rampant once again, destroys the ecological balance." 3R " problem of Here it is so-called chemical pesticide.
Botanical pesticide has low toxicity, noresidue to the safer characteristics of natural enemy; Simultaneously, botanical pesticide contains the number of chemical composition, is difficult for developing immunity to drugs.Therefore botanical pesticide is to solve the comparatively ideal class public nuisance-free agricultural chemicals of " 3R " problem, it is one of important means at green barrier in the international agriculture trade and production green and organic food, recent two decades comes, the research and development of botanical pesticide and application progressively obtain insider's attention, and obtain certain development.
The exploitation of botanical pesticide at present mainly contains dual mode, and the one, from plant, seek pesticide activity component, as the lead compound of the new chemical pesticide of synthesizing efficient low toxicity; The 2nd, direct appliable plant resource is made botanical pesticide.External first kind of mode that adopt more.Domestic because of plant resources enriches, how based on the second way.Existing part plant pesticide patent of China or product come out at present, but wherein the overwhelming majority is a plant insecticide, and it is then very few to relate to botanical fungicide.
The present invention finishes on a large amount of folk prescriptions screenings, composition optimization and the basis updated.Final selection cinnamon oil, grass-leaved sweetflag, kuh-seng, eucalyptus oil are the raw material combination thing.
Three, summary of the invention
The object of the present invention is to provide a kind of novel compound botanical fungicide that utilizes medicinal plant for feedstock production.The inventor is through a large amount of screenings and optimum organization, and selecting cinnamon oil, grass-leaved sweetflag, kuh-seng and eucalyptus oil is that the bactericide that raw material is made has very strong inhibitory action to multiple target pathogen.Even the unmanageable sickle-like bacteria of general agricultural chemicals is also had fungistatic effect preferably, this is that the other plant agricultural chemicals can not be compared.
The single preparations of ephedrine that cinnamon oil, grass-leaved sweetflag, shrubby sophora extract are made respectively all has fungistatic effect preferably, the results are shown in Table 1.The compound preparation drug effect is better than kuh-seng and grass-leaved sweetflag single preparations of ephedrine, but it is active strong to be not so good as the cinnamon oil single preparations of ephedrine.The adding eucalyptus oil can obviously improve the bacteriostatic activity of three kinds of compositions, the results are shown in Table 2, several advantages is arranged: 1) can obviously improve activity, especially sickle-like bacteria Fusarium sp. be had more satisfactory fungistatic effect after the adding.Still have goodish drug effect when 2) dilute concentration is 1000 * liquid, obviously be better than not adding the eucalyptus oil person.3) longevity of residure longer, the 6th day drug effect obviously is better than not adding the eucalyptus oil person.Therefore last this four plant species extract combination of selection is this compound composition.
Table 1 compound preparation and each single preparations of ephedrine fungistatic effect are relatively
*
*: each is handled medicament and is 1000 times (1000 *) of dilution
Have or not the antibacterial comparison of eucalyptus oil in the table 2 compound cinnamon oil preparation
Reason of this prescription and advantage following points:
1. the main active of this preparation has cinnamic acid, beta-Asarone, multiple matrine.The active component of number of different types is collaborative mutually, and action compensating can have strong inhibitory action to many target plant disease fungus, and fungicidal spectrum is wider.
2. the compound combination can enlarge stock number, can " high and low collocation " reduce cost simultaneously.
3. the combination of number of different types active component is difficult for developing immunity to drugs.
Technical scheme of the present invention is:
1. a kind of compound plant source bactericide and preparation method thereof.It is characterized in that comprising the working substance of following composition by weight raw material or extraction: 0.3~4 part of cinnamon oil, 1~6 part of grass-leaved sweetflag, 1~6 part of kuh-seng, 0.3~4 part of eucalyptus oil;
2. this bactericide can be mixed with missible oil, aqueous emulsion, the multiple formulation of microemulsion; The composition by weight ratio of various preparations is: 2~7 parts of missible oil-plant extractss, 1~5 part of solvent, 1~2.5 part of other auxiliary agent; 2~6 parts of aqueous emulsion and microemulsion-extracts, 0.5~4 part of solvent, 2~6 parts in water, 1~2.5 part of other auxiliary agent;
3. grass-leaved sweetflag and kuh-seng are pulverized, are extracted respectively or two kinds of raw materials mix and extract, obtain extract with solvent, through concentrate extract.The solvent that extracts can be the mixed solvent of methyl alcohol, ethanol, acetone, cyclohexane or other common solvent and several solvents.Cinnamon oil is by Chinese cassia tree water distillation extraction, and is also commercially available, and eucalyptus oil is by folium eucalypti water distillation extraction, and is also commercially available.
4. according to above-mentioned proportioning 1. with the synthetic total plant extracts of the extract mixture of 4 plant species.
5. according to above-mentioned proportioning 2. plant extracts and solvent, auxiliary agent are mixed and made into missible oil, aqueous emulsion, microemulsion several formulations.
The preparation method of above-mentioned botanical fungicide and step are:
1. the grass-leaved sweetflag and the kuh-seng of drying are pulverized, difference or mixing are extracted with solvent refluxing, extract 2 times, and extracted twice liquid merges.
2. use the method for vacuum concentration, extract is concentrated and be settled to crude drug weight 1/2, standby.
3. extract Chinese cassia tree respectively with conventional steam distillation and get cinnamon oil, extract folium eucalypti and get eucalyptus oil.Also can buy this two vegetable oil from market.
4. four kinds of extracts are pressed following important component, 0.3~4 part of cinnamon oil, 1~6 part of grass-leaved sweetflag, 1~6 part of kuh-seng, 0.3~4 part of eucalyptus oil is mixed into total plant extracts.
5. in reactor, by following composition by weight, i.e. plant extracts 2-7 part, solvent 1-5 part, auxiliary agent 1-2.5 part, mixing and stirring makes bactericide missible oil.
6. in reactor, press following composition by weight, plant extracts 2-6 part, solvent 0.5-4 part, auxiliary agent 1-2.5 part that each formulation is required, water 2-6 part stirs, and makes aqueous emulsion or microemulsion.
Pei Zhi plant pesticide can be used for by the microbial crop pest of various plants cause of disease as stated above.Be mainly used in the plurality of plant diseases that control is caused by Alternariaspp Alternaria sp., rhizoctonia solani Rhizoctonia solani, epidemic disease bacterium Phytophthora sp., Verticillium dahliae Verticillium sp., the long spore bacterium of crinosity dish Pestalotipassi sp., smut Ustilago sp..Can be used to prevent and treat medicinal plants such as genseng, American Ginseng, pseudo-ginseng, the bighead atractylodes rhizome, the bulb of fritillary, fructus amomi, chrysanthemum, matrimony vine, lily, Job's tears; Field crops such as wheat, corn, paddy rice, potato, sugarcane, beet, peanut, cotton; Vegetable crops such as Chinese cabbage, tomato, cucumber, pimento, capsicum, pea, French beans; Horticultural crops such as cherry, peach, pears, apple, oranges and tangerines, grape, strawberry; The multiple diseases such as flowers of kaffir lily, aloe.
Four, embodiment
For further understanding the present invention, below provide technical solution of the present invention embodiment, but be not limited to these embodiment.
Embodiment 1, extract preparation
Take by weighing grass-leaved sweetflag 30kg, kuh-seng 70kg mixes and pulverized 40 mesh sieves, soaks 24h in 400kg ethanol.Refluxing extraction in reactor is extracted 80 ℃ of temperature, and extraction time 3h filters, and extracts 2 times with method, and twice filtrate is merged, and concentrates and reclaims solvent to 1/2 of primary dose, promptly is settled to 50kg, obtains extract.
Buy cinnamon oil and eucalyptus oil from market, take by weighing cinnamon oil 5kg, eucalyptus oil 5kg, the extract 40kg of grass-leaved sweetflag and kuh-seng gets total plant extracts.
The preparation of embodiment 2, bactericide missible oil
In reactor, add total plant extracts 35kg, methyl alcohol, each 25kg of ethanol, alkylphenol polyoxyethylene class surfactant 9kg, bleeding agent 6kg stirs, and gets bactericide missible oil.
The preparation of embodiment 3, disinfectant aqueous emulsion and microemulsion
In reactor, add total plant extracts 35kg, each 10kg of methyl alcohol and ethanol, alkylphenol polyoxyethylene class surfactant 10kg, bleeding agent 5kg, water 35kg stirs, and promptly gets disinfectant aqueous emulsion or microemulsion.
Embodiment 4, disinfectant aqueous emulsion of the present invention are to antibacterial movable mensuration of plant pathogenic fungi
1, for the examination bacterial classification: eggplant stand withered bacterium Rhizoctonia solani, Folium Panacis Quinquefolii pinta Alternaria panax, graw mold of tomato Botrytis cinerea, notoginseng root rot Fusarium sp.,
2, experimental technique:
The mycelial growth rate method: every processing repeats for 3 times, repeats a ware at every turn, and every ware connects a bacterium cake, establishes clear water for contrast, handles and respectively adds up every ware colony diameter in back 3 days, 6 days, and calculate mycelial growth inhibition rate (%)
3, experimental result such as table 3
Table 3 compound cinnamon oil aqueous emulsion is to the fungistatic effect of several plant pathogen
Experimental result shows, said preparation all has very strong inhibitory action to eggplant rhizoctonia solani, American Ginseng leaf spot fungi, botrytis cinerea, notoginseng root rot bacterium, especially to eggplant rhizoctonia solani best results, especially the reaping hook germ that is difficult to tackle is also showed stronger bacteriostasis.This is that the other plant agricultural chemicals hardly matches.
Embodiment 5 disinfectant aqueous emulsion control Folium Panacis Quinquefolii pinta field test results of the present invention
Subjects: life in 2 years and the 4 years Western seedling leaf spot Alternaria of life panax,
Test method: 5 square metres of every experimental plot areas, every processing repeats for 3 times, establishes clear water and is contrast, and other establishes contrast chemical pesticide 70% mancozeb wettable powder.Spray medicine twice mid-June, be 7 days interval.The disease leaf is respectively distinguished in removal before spraying for the first time medicine, and a week is added up disease index behind the 2nd the spray medicine, and calculates control efficiency.
Result of the test: result of the test (seeing Table four) shows, the compound bactericide aqueous emulsion, and control American Ginseng life in 2 years and 4 years living seedling leaf spots all have good control efficiency, and are better than local medicine 70% mancozeb commonly used.
Table four compound bactericide aqueous emulsion control Folium Panacis Quinquefolii pinta field plot trial result
Claims (7)
1. compound plant source bactericide is characterized in that comprising the working substance of following composition by weight raw material or extraction:
0.3~5 part of cinnamon oil, 1~6 part of grass-leaved sweetflag, 1~6 part of kuh-seng, 0.3~4 part of eucalyptus oil
2. the preparation method of bactericide according to claim 1 is characterized in that this bactericide can be mixed with missible oil, aqueous emulsion, the multiple formulation of microemulsion.
3. according to the botanical fungicide of claim 2, the composition by weight that it is characterized in that various preparations is than being missible oil: 2~7 parts of plant extractss, 1~5 part of solvent, 1~2.5 part of aqueous emulsion of other auxiliary agents and microemulsion: 2~6 parts of extracts, 0.5~4 part of solvent, 2~6 parts in water, 1~2.5 part of other auxiliary agent
4. according to the preparation method of the described bactericide of claim 1, it is characterized in that extracting kuh-seng, two kinds of raw materials of grass-leaved sweetflag or 2 kinds of raw materials mixing back extractions respectively with extracting solvent, with extract concentrate extract, cinnamon oil is by Chinese cassia tree water distillation extraction, also commercially available, eucalyptus oil is by folium eucalypti water distillation extraction, and is also commercially available.
5. the preparation method of bactericide according to claim 4, wherein said extraction solvent can be methyl alcohol, ethanol, acetone, cyclohexane or other common solvent, also can be the mixed solvent of several solvents.
6. according to the purposes of the bactericide described in the claim 1-3, it is characterized in that to be used for medicinal plants such as genseng, American Ginseng, pseudo-ginseng, the bighead atractylodes rhizome, the bulb of fritillary, fructus amomi, chrysanthemum, matrimony vine, lily, Job's tears; Field crops such as wheat, corn, paddy rice, potato, sugarcane, beet, peanut, cotton; Vegetable crops such as Chinese cabbage, tomato, cucumber, pimento, capsicum, pea, French beans; Horticultural crops such as cherry, peach, apple, pears, oranges and tangerines, grape, strawberry; Flowers such as kaffir lily, aloe.
7. according to the purposes of the bactericide described in the claim 1-3, it is characterized in that to be used to preventing and treating by Alternaria Alternariasp., eggplant upright withered bacterium Rhizoctonia solani, epidemic disease bacterium Phytophthora sp., anthrax bacteria Colletotrichum sp., sickle-like bacteria Fusarium sp., botrytis sp Botrytis cinerea, stem and put the plurality of plant diseases that mould Phoma sp., Verticillium dahliae Verticilliumsp., the long spore bacterium of crinosity dish Pestalotipassi sp., smut Ustilago sp. cause.
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