CN116918838B - Application of cinnamon essential oil in preventing and treating plant diseases - Google Patents
Application of cinnamon essential oil in preventing and treating plant diseases Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Microbiology (AREA)
- Mycology (AREA)
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- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
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Abstract
The invention belongs to the technical field of plant disease prevention and control, and particularly relates to application of cinnamon essential oil in plant disease prevention and control. The plant diseases controlled by the cinnamon essential oil are root rot or anthracnose, and the bacteriostatic tests prove that the bacteriostatic rates of the cinnamon essential oil on onion anthracnose and ash tree anthracnose are 81.11 percent, 80.95 percent respectively, and the bacteriostatic rates of the cinnamon essential oil on Fusarium solani, fusarium oxysporum and Fusarium finerium are 49.64 percent, 21.43 percent and 14.98 percent respectively under 2000 times of dilution concentration, and the bacteriostatic rates of the cinnamon essential oil on 5 pathogenic bacteria are 100 percent under smaller dilution concentration.
Description
Technical Field
The invention belongs to the technical field of plant disease prevention and control, and particularly relates to application of cinnamon essential oil in plant disease prevention and control.
Background
The plant essential oil is a natural antibacterial agent, called as gold liquid, and is mainly extracted from plant structures such as petals, buds, leaves, branches, resin and the like of natural plants. Plant essential oils are generally fragrant, often because of their unique taste, which is favored by many people. Besides the fragrance, the essential oil has a plurality of active functions, such as antioxidation, aging delay, pathogenic microorganism resistance and the like, so that the essential oil gradually enters the public field of view, more researchers are more and more, and the application range is wider and wider.
Research shows that the plant essential oil can affect the cell membrane and cell wall of fungi, so that the permeability of the cell membrane of the fungi is increased, and the integrity of the cells is lost, so that the cells of the fungi die. Plant essential oils can also affect the synthesis of fungal nucleic acids and proteins and inactivate fungal cells by affecting the expression level of mycotoxin synthesis genes.
Root rot is a name that plant roots are decayed and cannot normally absorb moisture, inorganic salts and other nutrient substances from soil, so that the plant roots are weakened and finally die. The main symptoms of root rot are yellowing, wilting and root rot of the whole plant leaves. It is a plant disease caused by the combined action of many nematodes, fungi and bacteria, so its etiology is complex and it cannot be treated by simply inhibiting one pathogen. The diseased plants can also be infected by other saprophytic bacteria, complex symptoms appear, and symptoms are aggravated.
The pathogenic matters causing the root rot of the rhizoma polygonati are mainly fungi such as fusarium oxysporum, fusarium putrescens and the like. At present, in order to improve the yield of the rhizoma polygonati, spraying chemical bactericides is a main measure for preventing and treating the root rot of the rhizoma polygonati. However, chemical bactericides have problems of toxicity and pesticide residues. This results in the presence of chemical bactericides in commercially available sealwort. Eating rhizoma Polygonati with pesticide residue is very easy to harm human health, and chemical bactericide will also cause some harm to the environment.
Based on the above reasons, the invention aims to prevent and treat the Polygonatum sibiricum root rot by a biological method and reduce the influence of chemical bactericides on the environment. The plant essential oil is a natural, green and pollution-free substance extracted from various plants and has good antibacterial activity, has remarkable effect on sterilization, has the characteristics of biodegradation, easy volatilization, low concentration residue and the like, and is environment-friendly, so the invention aims to seek a plant essential oil with better control effect on the rhizoma polygonati root rot.
Disclosure of Invention
Based on the technical purpose, the invention provides the application of cinnamon essential oil in preventing and treating plant diseases, wherein the diseases are root rot or anthracnose. Antibacterial tests prove that the cinnamon essential oil has good inhibition effects on onion anthracnose, white wax tree anthracnose, fusarium putrescens, fusarium oxysporum and Fusarium fingium which cause plant root rot.
The technical scheme provided by the invention is as follows:
the invention provides an application of cinnamon essential oil in preventing and treating plant diseases, wherein the diseases are root rot or anthracnose, and the cinnamon essential oil is extracted according to the following steps: extracting cortex Cinnamomi by steam distillation, and collecting essential oil.
Preferably, the cinnamon essential oil is used for inhibiting onion anthracnose, white wax tree anthracnose, fusarium oxysporum, fusarium putrescens or fusarium fingium.
Preferably, the plant is Polygonatum sibiricum.
Preferably, the dosage ratio of the cinnamon medicinal material to the water is 1g to 20 g mL.
Preferably, the cinnamon essential oil is formulated as a medicament.
Preferably, the medicament further comprises an agropharmaceutically acceptable adjuvant.
Preferably, the medicament is 250-2000 times of the diluent of cinnamon essential oil.
Preferably, the medicament is 250-1000 times of the diluent of cinnamon essential oil.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides application of cinnamon essential oil in preventing and treating plant diseases. Wherein, the diseases controlled by cinnamon essential oil are root rot and anthracnose. Antibacterial tests prove that under 2000 times of dilution concentration, the cinnamon essential oil has antibacterial rates of 81.11 percent and 80.95 percent for onion anthracnose and white wax tree anthracnose respectively, and has antibacterial rates of 49.64 percent, 21.43 percent and 14.98 percent for Fusarium putrescens, fusarium oxysporum and Fusarium finerium respectively; and under the condition of smaller dilution concentration, the inhibition rate of the cinnamon essential oil on 5 pathogenic bacteria reaches 100 percent.
Drawings
FIG. 1 shows the inhibitory effect of cinnamon essential oil on onion anthracnose; and (3) injection: in the figure, a to e are CK, 2000 times, 1000 times, 500 times and 250 times in sequence;
FIG. 2 is the inhibitory effect of cinnamon essential oil on Ceratoxylum bungeanum; and (3) injection: in the figure, a to e are CK, 2000 times, 1000 times, 500 times and 250 times in sequence;
FIG. 3 shows the inhibitory effect of cinnamon essential oil on Fusarium oxysporum; and (3) injection: in the figure, a to e are CK, 2000 times, 1000 times, 500 times and 250 times in sequence;
FIG. 4 shows the inhibitory effect of cinnamon essential oil on Fusarium putrescens; and (3) injection: in the figure, a to e are CK, 2000 times, 1000 times, 500 times and 250 times in sequence;
FIG. 5 is the effect of cinnamon essential oil on Fusarium fineri inhibition; and (3) injection: in the figure, a to e are CK, 2000 times, 1000 times, 500 times and 250 times in sequence;
fig. 6 is a graph showing the inhibitory effect of cinnamon essential oil on different pathogens.
Detailed Description
The invention will be further illustrated with reference to specific examples, which include but are not limited to.
1. Material
1.1, test plants and seed
Cinnamon bark, purchased in the Hebei Anguo market.
Onion anthracnose pathogenColletotrichum circinans) Rhizopus chinensisC. spaethianum) Fusarium oxysporum (F.oxysporum)Fusarium oxysporum) Fusarium solani (Fusarium solani)F. solani) Fusarium fingium (F. Fusarium)F. redolens) Are all isolated from the root rot of Polygonatum sibiricum and stored in the quality detection laboratory of traditional Chinese medicine in Taishan college.
1.2 laboratory instruments and reagents
GI54T vertical automatic pressure steam sterilization pot (micro-mansion instruments ltd); VA214 electronic balance (Shanghai Jing day electronics limited); BHC-1300IIA2 ultra clean bench (alctai laboratory equipment beijing limited); a water bath kettle; electrothermal sleeve (Shanghai Instrument & instrument Co., ltd.); essential oil extractor (base company).
Potato Dextrose Agar (PDA) and tween-20 are all of domestic analytical purity.
2. Method of
2.1 extraction of essential oil of cinnamon
Weighing 100 g of cortex Cinnamomi Japonici, and extracting essential oil four times. Taking 25 g medicinal materials into a 1000 mL round bottom flask, adding 500 mL distilled water according to the ratio of 1:20, and stirring with a glass rod to make the water overflow the materials. The rubber tube connected with the lower end of the condensing tube is connected with the water tap, so that the entire condensing tube is filled with water, and the water flows out from the upper outlet of the condensing tube. The condensing tube is connected with the essential oil extractor, and the essential oil extractor is fixed by an iron stand. The round bottom flask was placed in an electric mantle and heated slowly, the voltage was adjusted to 220V, and when the essential oil began to drip, the micro-boiling was maintained for 2 h until the amount of essential oil no longer increased and the heating was stopped. After the device is cooled, a piston at the lower end of the essential oil extractor is opened, the essential oil is slowly discharged, the essential oil in the extractor is collected into a centrifuge tube, and the volume of the essential oil is recorded. Repeating the above steps, and extracting essential oil from the rest 75g of cortex Cinnamomi Japonici. Mixing the four extracted essential oils to obtain cortex Cinnamomi essential oil stock solution, and storing in shade.
2.2 bacteriostasis experiments of plant essential oil on root rot fungi
2.2.1 activation of fungi
5.7744 g PDA culture medium was weighed into a conical flask, 144 mL distilled water was added, the mouth was closed, and the flask was placed in a sterilizer. The system was adjusted and sterilized at 121℃for 20 minutes. In an ultra clean bench, PDA medium which has been sterilized is poured evenly into the petri dish near the sterile field of the flame. Repeatedly burning the sterilized inoculating needle in the outer flame of an alcohol lamp, picking up a piece of beaten fungus sheet with the inoculating needle after cooling, placing the fungus sheet at the right center of a PDA culture medium, sealing, placing the fungus sheet in an incubator at 26 ℃ for inversion culture, and culturing for 7 days.
2.2.2 preparation of culture Medium
PDA culture medium formula: 40.1 g PDA culture medium powder is added into 1000. 1000 mL distilled water, and the mixture is placed in an autoclave for sterilization at 121 ℃ for 20 minutes.
Adding tween-20 with mass fraction of 0.5% into cortex Cinnamomi essential oil stock solution, emulsifying, and diluting to four concentration gradients of 10 times, 20 times, 40 times and 80 times.
Dilution procedure 10-fold: adding 1.5 mL cortex Cinnamomi essential oil stock solution into 50 mL No. 1 centrifuge tube, adding 1 mL Tween-20, and adding 12.5 mL double distilled water to obtain 15 mL mother solution 1.
Dilution procedure 20 times: mother liquor 1 of 7 mL was added to a 50 mL centrifuge tube No. 2, and double distilled water of 7 mL was added to prepare mother liquor 2 of 14 mL.
Dilution procedure 40-fold: mother liquor 2 of 7 mL was added to a No. 3 centrifuge tube of 50 mL, and double distilled water of 7 mL was added to prepare mother liquor 3 of 14 mL.
80-fold dilution process: mother liquor 3 of 7 mL was added to a No. 4 centrifuge tube of 50 mL, and double distilled water of 7 mL was added to prepare mother liquor 4 of 14 mL.
Cooling sterilized PDA culture medium to 50deg.C, adding diluted gradient essential oil mother liquor, and mixing thoroughly to dilute the essential oil concentration to 250 times, 500 times, 1000 times, and 2000 times.
250-fold dilution process: mother liquor 1 of 6 mL was added to PDA medium of 144 mL to prepare 150 mL.
500-fold dilution process: mother liquor 2 of 6 mL was added to PDA medium of 144 mL to prepare 150 mL.
1000-fold dilution process: mother liquor 3 of 6 mL was added to PDA medium of 144 mL to prepare 150 mL.
2000-fold dilution procedure: mother liquor 4 of 6 mL was added to PDA medium of 144 mL to prepare 150 mL.
The prepared PDA medium containing essential oil was poured into a 90 mm petri dish, and the height of the poured medium was approximately one third of the height of the petri dish.
2.2.3 antibacterial operation steps
Burning the strain on an alcohol lamp for a period of time by using a puncher which is heated and sterilized, and after the temperature is reduced, laying a sufficient number of bacterial slices on an activated strain culture dish. The fungus pieces are picked by an inoculating needle and are connected to the center of the culture dish. Each gradient was repeated three times. And (5) placing the culture dish with the inoculated bacteria in an incubator in a sealing way, and culturing for 5-7 d in an inverted way. When colonies in the dish grew to two thirds of the dish, the diameter of the colonies was measured.
3. Data processing
When colonies grow to two thirds of the dishes, the diameter is measured by the crisscross method, and the data are photographed and recorded. And calculating the average value of colony diameters at each concentration by using Excel, and finally obtaining the antibacterial rate.
Antibacterial ratio (%) = (average diameter of control colony-6 mm) - (average diameter of treated colony-6 mm)/(average diameter of control colony-6 mm) ×100%.
EC 50 : i.e. half the bacteriostatic concentration, also called half maximal effect concentration, EC 50 Are often used as drug safety indicators. It means that the concentration of the drug has a certain obvious effect on 50% of individuals of the tested strain. The term "test" refers to the concentration of essential oil that causes 50% of individuals of the test species to be inhibited.
The measured colony diameters of the strains at the concentrations are recorded in a table, the average colony diameters of the strains at the different concentrations are calculated, and the antibacterial rate is calculated by using the values.
And finding out the corresponding probability value in the probability value conversion table according to the obtained antibacterial rate. Finally, taking the logarithmic value LogC of the essential oil concentration as an X axis, taking the several values of the essential oil concentration as a Y axis, selecting the numerical value areas corresponding to the X axis and the Y axis, and inserting Excel into a scatter diagram to enable the obtained diagram to display a virulence regression equation Y=aX+b formula, a straight line trend and R 2 Values. R is R 2 The closer to 1, the stronger the correlation between the values is explained. The objection value of the X value obtained after the y=5.0 is brought into the virulence regression equation is EC 50 Values. By EC (EC) 50 The value is obtained to obtain the antibacterial capacity of the essential oil.
4. Results and analysis
4.1 extraction of essential oil
The extraction rate of the cinnamon essential oil extracted by the experiment is about 1%, the cinnamon essential oil is extracted to be 6 mL, the color of the cinnamon essential oil is yellow, and the plant smell of the essential oil is rich.
4.2, the inhibition effect of cinnamon essential oil on 5 bacteria
From the average colony diameter under the control group, it can be seen that: under the condition of the same culture days, the bacterial colonies of the white waxy tree anthracnose, the fusarium oxysporum and the fusarium putrescens are larger, the average diameters of the bacterial colonies are respectively 62 mm, 48 mm and 56.3 mm, the bacterial colonies of the onion anthracnose and the fusarium finnish are similar in size, and the average diameters of the control group are all 30.7 mm. From fig. 1 to 6, it is known that the cinnamon essential oil has particularly obvious inhibition effects on two anthracnose pathogens, and the inhibition effects on onion anthracnose pathogens and ash tree anthracnose pathogens are only different under the condition that the essential oil is diluted 2000 times, and the inhibition rates are 81.11% and 80.95% respectively. The inhibition effect on Fusarium putrescens is moderate, the inhibition effect on Fusarium oxysporum and Fusarium finyi is not obvious, but the inhibition rate of the cinnamon essential oil on Fusarium putrescens, fusarium oxysporum and Fusarium finyi is 49.64%, 21.43% and 14.98% respectively under three concentration gradients of 250 times, 500 times and 1000 times and the dilution gradient of 2000 times.
Based on the derived EC 50 The arrangement sequence of the values that the inhibition effect of the cinnamon essential oil on five bacteria is from excellent to poor is as follows: colletotrichum onion>Anthrax bailii>Fusarium putrescens>Fusarium oxysporum>Fusarium fingium, its corresponding EC 50 Values are 0.000121960 mL/L (8199-fold dilution), 0.000122951 mL/L (8133-fold dilution), 0.000251550 mL/L (3975-fold dilution), 0.000375161 mL/L (2665-fold dilution), 0.000414386 mL/L (2413-fold dilution), respectively, table 1.
TABLE 1 EC of various bacteria under cinnamon essential oil 50 Value of
| Bacterial strain | Toxicity regression equation | R² | EC 50 （mL/L） | Corresponding multiple |
| Colletotrichum onion | y=2.8277x+16.067 | 0.6 | 0.000121960 | 8199 times |
| Anthrax bailii | y=2.8351x+16.086 | 0.6 | 0.000122951 | 8133 times |
| Fusarium oxysporum | y=4.4962x+20.403 | 0.6 | 0.000375161 | 2665 times |
| Fusarium putrescens | y=3.7162x+18.376 | 0.6 | 0.000251550 | 3975 times of |
| Fusarium fingium | y=4.7434x+21.045 | 0.6 | 0.000414386 | 2413 times of |
The inhibition effect of the cinnamon essential oil on the anthracnose is better than that of fusarium. The inhibition effect on Fusarium fingium is the worst, and the inhibition effect on onion anthracnose is the best. Under the conditions of 250 times, 500 times and 1000 times dilution, the inhibition effect of the cinnamon essential oil on 5 bacteria is 100%, and the cinnamon essential oil has bacteriostasis difference only on 2000 times.
The above embodiment is only one of the preferred embodiments of the present invention, and should not be used to limit the scope of the present invention, but all the insubstantial modifications or color changes made in the main design concept and spirit of the present invention are still consistent with the present invention, and all the technical problems to be solved are included in the scope of the present invention.
Claims (4)
1. The application of cinnamon essential oil in preventing and controlling plant diseases is characterized in that the diseases are anthracnose, the plants are rhizoma polygonati, and the cinnamon essential oil is used for inhibiting onion anthracnose bacteria or ash tree anthracnose bacteria; the cinnamon essential oil is extracted according to the following steps: extracting cortex Cinnamomi by steam distillation, and collecting essential oil.
2. The use according to claim 1, wherein the ratio of cinnamon material to water is 1 g:20-30 mL.
3. The use according to claim 1, wherein the cinnamon essential oil is formulated as a medicament, the medicament further comprising an agropharmaceutically acceptable adjuvant, the medicament being a 250-2000 fold dilution of the cinnamon essential oil.
4. The use according to claim 3, wherein the medicament is a 250-1000 fold dilution of cinnamon essential oil.
Priority Applications (1)
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