CN117941700B - Botanical fungicide for preventing and treating black spot of pseudo-ginseng - Google Patents
Botanical fungicide for preventing and treating black spot of pseudo-ginseng Download PDFInfo
- Publication number
- CN117941700B CN117941700B CN202410349331.XA CN202410349331A CN117941700B CN 117941700 B CN117941700 B CN 117941700B CN 202410349331 A CN202410349331 A CN 202410349331A CN 117941700 B CN117941700 B CN 117941700B
- Authority
- CN
- China
- Prior art keywords
- pseudo
- ginseng
- black spot
- alternaria alternata
- evodiamine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000131316 Panax pseudoginseng Species 0.000 title claims abstract description 45
- 235000003181 Panax pseudoginseng Nutrition 0.000 title claims abstract description 41
- 206010027146 Melanoderma Diseases 0.000 title claims abstract description 28
- 239000000417 fungicide Substances 0.000 title abstract description 7
- 230000000855 fungicidal effect Effects 0.000 title abstract description 5
- ABDKAPXRBAPSQN-UHFFFAOYSA-N veratrole Chemical compound COC1=CC=CC=C1OC ABDKAPXRBAPSQN-UHFFFAOYSA-N 0.000 claims abstract description 42
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 37
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 34
- 239000003899 bactericide agent Substances 0.000 claims abstract description 33
- 241000223602 Alternaria alternata Species 0.000 claims abstract description 28
- HMXRXBIGGYUEAX-SFHVURJKSA-N Evodiamine Natural products CN1[C@H]2N(CCc3[nH]c4ccccc4c23)C(=O)c5ccccc15 HMXRXBIGGYUEAX-SFHVURJKSA-N 0.000 claims abstract description 22
- TXDUTHBFYKGSAH-SFHVURJKSA-N Evodiamine Chemical compound C1=CC=C2N(C)[C@@H]3C(NC=4C5=CC=CC=4)=C5CCN3C(=O)C2=C1 TXDUTHBFYKGSAH-SFHVURJKSA-N 0.000 claims abstract description 22
- 201000010099 disease Diseases 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 6
- 241000180649 Panax notoginseng Species 0.000 claims description 4
- 235000003143 Panax notoginseng Nutrition 0.000 claims description 4
- 230000003385 bacteriostatic effect Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 30
- 241000196324 Embryophyta Species 0.000 abstract description 27
- 230000005764 inhibitory process Effects 0.000 abstract description 14
- 238000004382 potting Methods 0.000 abstract description 8
- 231100000419 toxicity Toxicity 0.000 abstract description 7
- 230000001988 toxicity Effects 0.000 abstract description 7
- 238000005259 measurement Methods 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 3
- 231100000674 Phytotoxicity Toxicity 0.000 abstract description 2
- 239000000575 pesticide Substances 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 18
- 230000002265 prevention Effects 0.000 description 11
- 239000003814 drug Substances 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000001965 potato dextrose agar Substances 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 244000052616 bacterial pathogen Species 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000266349 Alternaria tenuissima Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 239000005760 Difenoconazole Substances 0.000 description 2
- 229930189092 Notoginsenoside Natural products 0.000 description 2
- 235000002791 Panax Nutrition 0.000 description 2
- 241000208343 Panax Species 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- BQYJATMQXGBDHF-UHFFFAOYSA-N difenoconazole Chemical compound O1C(C)COC1(C=1C(=CC(OC=2C=CC(Cl)=CC=2)=CC=1)Cl)CN1N=CN=C1 BQYJATMQXGBDHF-UHFFFAOYSA-N 0.000 description 2
- QBKSWRVVCFFDOT-UHFFFAOYSA-N gossypol Chemical compound CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 QBKSWRVVCFFDOT-UHFFFAOYSA-N 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
- 229930182490 saponin Natural products 0.000 description 2
- 150000007949 saponins Chemical class 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- YXHVCZZLWZYHSA-UHFFFAOYSA-N (Z)-6-[8-pentadecenyl]salicylic acid Natural products CCCCCCC=CCCCCCCCC1=CC=CC(O)=C1C(O)=O YXHVCZZLWZYHSA-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000223600 Alternaria Species 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- YXHVCZZLWZYHSA-FPLPWBNLSA-N Ginkgoic acid Chemical compound CCCCCC\C=C/CCCCCCCC1=CC=CC(O)=C1C(O)=O YXHVCZZLWZYHSA-FPLPWBNLSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229930000755 gossypol Natural products 0.000 description 1
- 229950005277 gossypol Drugs 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the field of biological pesticides, in particular to a botanical fungicide for preventing and treating pseudo-ginseng black spot disease, and specifically the invention provides a scheme that evodiamine or veratrole is applied to preventing and treating pseudo-ginseng black spot disease caused by alternaria alternata (ALTERNARIA ALTERNATA), and the invention shows that the two botanical fungicides of the evodiamine and the veratrole have stronger inhibition effect on alternaria alternata (ALTERNARIA ALTERNATA) through an indoor fungicide toxicity measurement result; and the indoor potting experiments show that the two plant source bactericides have no phytotoxicity to the pseudo-ginseng under different gradient concentrations, and the pseudo-ginseng grows normally.
Description
Technical Field
The invention relates to the field of biological pesticides, in particular to a botanical fungicide for preventing and treating pseudo-ginseng black spot.
Background
Notoginseng radix (Panax notoginseng) is plant of Panax of Araliaceae, and has special requirement for growth environment, and natural distribution area is mainly in Yunnan. Dried root and rhizome of pseudo-ginseng are used as Chinese medicinal materials, are originally carried in the compendium of materia medica, and are one of the large-scale Chinese medicinal materials widely used in China. Modern medicine has made a great deal of researches on the components, pharmacological actions and clinical application of notoginsenoside, the development of stems and leaves of the notoginsenoside is more and more, the demand of the pseudo-ginseng is rapidly increased, and the pseudo-ginseng planting industry is rapidly developed. The problem of serious diseases and insect pests of the pseudo-ginseng is accompanied with large-scale planting, so that the yield and quality of the pseudo-ginseng are reduced, and the healthy development of the pseudo-ginseng industry is hindered. It has been found that the variety of diseases of pseudo-ginseng reaches more than 20, and the diseases of root rot, black spot, round spot and the like occur more commonly and seriously, which causes huge loss of pseudo-ginseng planting industry each year. The perennial incidence rate of the black spot disease of the pseudo-ginseng is between 5 and 20 percent, and the incidence rate reaches more than 60 percent when the black spot disease is serious. The germs can infect each part of the pseudo-ginseng plant, and the damage of the stems, the leaf stalks and the flower shafts is heavy. Most of leafstalk infections start to generate nearly circular or irregular water-immersed brown disease spots between leaf tips and leaves, and then gradually spread to form rotten pseudo-ginseng, so that the whole plant dies. The infection source of the pseudo-ginseng black spot disease is mainly Alternaria fungi, researches of the dynasty beam and the like show that the pathogen of the pseudo-ginseng black spot disease is Alternaria tenuissima (ALTERNARIA ALTERNATA KEISSL), wang Shuqin and the like consider that the pseudo-ginseng black spot disease is caused by the infection of Alternaria tenuissima (ALTEMARIA PANAX WHETZEL), and researches of the packaging rise show that the pathogen of the pseudo-ginseng black spot disease in the Yunnan region is ALTERNARIA ALTERNATA, and germ spores can overwinter in the diseased stems and leaves and become the main primary infection source in the next year.
In production, the prevention and treatment means for the black spot disease of the pseudo-ginseng are many, but chemical prevention and treatment are mainly used at present, so far, the number of medicines registered for the black spot disease of the pseudo-ginseng is less than 10, the chemical medicines are mainly used, and the most registered quantity of difenoconazole is the most commonly used medicine. The long-term use of chemical bactericides leads to gradual enhancement of drug resistance of germs, and the continuous use of the chemical reagents also causes environmental pollution, and residues of the chemical reagents on medicinal plants threaten the physical health of people through food chains. Based on the above, the screening of novel agents with high biological control activity and environmental safety has become an urgent practical requirement, and plant-source bactericides, which are extracted from plants, contain components with antibacterial, bacteriostatic and insecticidal actions, have the characteristics of high efficiency, low toxicity, easy degradation in nature and the like, are the main direction of screening and research at present, and have been reported freshly against plant-source bactericides related to pseudo-ginseng black spot.
Disclosure of Invention
The invention mainly aims to provide a plant source bactericide for preventing and treating pseudo-ginseng black spot disease, which solves the problems in the background technology.
In order to achieve the above object, the present invention provides the following technical solutions.
The invention provides a scheme through screening: application of evodiamine or veratrole in preventing and treating pseudo-ginseng black spot caused by alternaria alternata (ALTERNARIA ALTERNATA)
Further, the antibacterial concentration of the evodiamine is not less than 34.70 mg/L.
Further, the antibacterial concentration of veratrole is not less than 42.99mg/L.
Further, the invention provides a method for preventing and treating the black spot of pseudo-ginseng, which uses a bactericide containing evodiamine or veratrole to be sprayed on pseudo-ginseng plants in the initial stage of disease.
The invention achieves the technical effects that:
According to the invention, an indoor bactericide toxicity measurement result shows that the evodiamine and veratrole plant source bactericides have a strong inhibition effect on ALTERNARIA ALTERNATA pathogens, wherein the EC 50 of the evodiamine is 34.70 mg/L, the EC 50 of the veratrole is 42.99mg/L, an indoor potting pesticide effect test shows that the control effect of the evodiamine at the concentration of 100 mg/L is superior to that of the veratrole, the control effect of the evodiamine and the veratrole at the concentration of 300mg/L is close, and the two plant source bactericides do not generate crude drug harm on pseudo-ginseng under different gradient concentrations through the observation of the indoor potting test, and the pseudo-ginseng grows normally.
Detailed Description
The present invention is further illustrated below by reference to examples, which are only examples illustrating the present invention and do not limit the scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
The test agents and the relevant information are shown in Table 1, and the test agents are diluted with 0.1% Tween-80 aqueous solution after being dissolved in an organic solvent or water according to the manufacturer's instructions.
TABLE 1 test agents
EXAMPLE 1 bacteriostatic Activity of different plant-derived bactericides
1. Test strain: the pathogenic bacteria ALTERNARIA ALTERNATA of the black spot disease of the pseudo-ginseng are separated, identified and stored by the subject group (refer to Bao Sheng for separation method, namely, secondary metabolite of the pathogenic bacteria ALTERNARIA ALTERNATA of the black spot disease of the pseudo-ginseng, and pathogenicity research [ D ] university of Yunnan, 2020.), and the test culture medium is potato dextrose agar culture medium (PDA: 200g of potato, 20 g of dextrose, 18g of agar powder, and water is added to a volume of 1L, and the mixture is sterilized and cooled under high pressure for 20 minutes for standby).
2.Alternaria alternata activation culture
And (3) using a PDA culture medium, culturing the separated and purified bacteria in a constant temperature incubator at 25 ℃ alternately for 5 days with a photoperiod L/D=12h/12h, and taking out bacterial cakes for standby at colony positions with similar bacterial ages and consistent growth vigor by using a sterilization puncher with the diameter of 5 mm.
3. Indoor toxicity measurement for pseudo-ginseng black spot disease
The growth rate method is adopted. The preparation to be tested is respectively prepared into 5 different mass concentration gradients, each test preparation is added into PDA plate culture medium cooled to about 50 ℃ to prepare a medicine-containing plate, a single bacterial cake with the diameter of 5mm is inoculated, sterile water is used as a control, and each treatment is repeated for 3 times. Culturing in a constant temperature incubator at 28 ℃, measuring the diameter of a bacterial colony by adopting a crisscross method after 5 d ℃, calculating the inhibition rate of different bactericides and different mass concentrations of the bactericides on bacterial hypha growth, and calculating the correlation coefficient and EC 50 of ALTERNARIA ALTERNATA under the treatment of the tested bactericides, wherein the calculation formula is as follows:
hypha growth inhibition ratio = [ (control group colony diameter-treatment group colony diameter)/control group colony diameter ] ×100%.
4. Test results
The result of the inhibition effect of the 5 plant source bactericides on ALTERNARIA ALTERNATA growth is shown in table 2, the inhibition rate of the 5 plant source bactericides is increased along with the increase of the use concentration, wherein the inhibition effect of evodiamine on ALTERNARIA ALTERNATA is strongest, and the inhibition effect is 62.54% at most when the mass concentration of the evodiamine is 80 mg/L; the effect of veratrole is inferior, and when the mass concentration of veratrole is 80mg/L, the highest inhibition effect on ALTERNARIA ALTERNATA is 58.42%; the tea saponin has the worst inhibition effect, and when the mass concentration is 80mg/L, the growth inhibition rate of ALTERNARIA ALTERNATA is only 38.20 percent.
The toxicity regression equation, the correlation coefficient and EC 50 of the 5 plant source bactericides to ALTERNARIA ALTERNATA are calculated through a standard curve, the results are shown in table 3, the correlation coefficient of the toxicity regression equation of the 5 plant source bactericides is higher than 0.97, the inhibition effect of the bactericides concentration and ALTERNARIA ALTERNATA is positively correlated, wherein the EC 50 of evodiamine is 34.70mg/L, and compared with other bactericides, the value is minimum, so that the inhibition effect of evodiamine to ALTERNARIA ALTERNATA growth is the best; the antibacterial effect of veratrole is inferior, and EC 50 is 42.99mg/L. Referring to EC 50 of ALTERNARIA ALTERNATA plant-derived bactericides, it is known that the inhibition effect of the effective components of 5 plant-derived bactericides on ALTERNARIA ALTERNATA is that evodiamine, veratrole, ginkgolic acid, gossypol and tea saponin are sequentially from strong to weak.
Inhibition effect of 25 plant source bactericides on ALTERNARIA ALTERNATA growth
Note that different lower case letters in the same column of data represent the significance of the difference at the 0.05 level for the different treatment groups, as follows.
TABLE 35 toxicity analysis of plant-derived fungicides on ALTERNARIA ALTERNATA
EXAMPLE 2 potted plant test
1. Test method
The potting test is carried out in a greenhouse, and the potting method is adopted to determine the prevention effect of the bactericide on the black spot of pseudo-ginseng. Conidia of 7 d were collected ALTERNARIA ALTERNATA and cultured on PDA medium in a 1.5 mL sterile centrifuge tube, and sterile water was added to make a spore suspension (1.0×10 5/mL). Transplanting into basin (diameter 33 cm ×20: 20 cm), growing three years of seedlings with consistent growth vigor, and injecting 10 μl spore suspension into the basal part of stem. The initial stage of the generation of the black spot disease of the pseudo-ginseng (oval brown spot appears), a constant sprayer is used for spraying bactericides with different concentrations on the root, the stem base and the leaf surface of the pseudo-ginseng, the dosage is about 10mL per plant based on the condition that the two surfaces of the leaf are wet and the liquid medicine does not drop down, the control group is treated by using sterile water, and difenoconazole for preventing and treating the black spot disease of the pseudo-ginseng is used as a commodity bactericide control. Each treatment was performed in 10 pots, 3 plants per pot were completely randomly arranged, 3 replicates were performed, and the treated pseudo-ginseng plants were cultivated in a greenhouse (room temperature at 24.+ -. 1 ℃ C., relative humidity 80%, L/D=12 h/12 h, after cultivation 10D, the test results were investigated.
2. Investigation method
All leaves of pseudo-ginseng are investigated, the total leaf number is recorded according to the percentage of the area of the disease spots to the area of the whole leaf, the leaf numbers of all stages of diseases are recorded, and the control effect is calculated according to the disease indexes of each treatment before and after the application.
Disorder grading criteria:
0. Stage: the whole plant is free from diseases;
1. stage: the area of the disease spots accounts for less than 1% of the leaf area;
3. Stage: 1/4 of the leaves are infected, and the area of the disease spots accounts for 2% -5% of the area of the whole leaves;
5. stage: 1/3 of the leaves are infected, and the area of the disease spots accounts for 6-10% of the area of the whole leaves;
7. stage: half of the leaves are infected, the disease spots start to merge, and the area of the disease spots accounts for 11% -20% of the area of the whole leaves;
9. stage: half of the leaves are infected, the lesions start to merge, and the area of the lesions accounts for more than 21% of the area of the whole leaf.
And calculating the disease index and the control effect according to the grading standard. The calculation formula is as follows:
3. test results
As shown in Table 4, the potted plant experiments show that the prevention effect of the evodiamine is 40.27%, 65.39% and 84.35% respectively at the concentrations of 50 mg/L, 100 mg/L and 300 mg/L, the prevention effect of the veratrole is 34.28%, 53.83% and 82.45% respectively at the concentrations of 50 mg/L, 100 mg/L and 300 mg/L, the prevention effect of the two plant source bactericides is synergistic with the increase of the concentration of the active ingredients, the prevention effect of the evodiamine is slightly better than the prevention effect of the veratrole at the low concentration, and when the concentration of the two plant source bactericides reaches 300 mg/L, the prevention effect is not obviously distinguished (P is less than 0.05), and the prevention effect of the evodia-zole is higher than 10 mg/L.
TABLE 4 prevention and treatment effects of test agent on black spot of Notoginseng radix
In conclusion, the toxicity measurement result of the indoor bactericide shows that the evodiamine and veratrole botanical bactericides have strong inhibition effect on ALTERNARIA ALTERNATA bacteria; the indoor potting test shows that the control effect of the evodiamine is better than that of veratrole at the concentration of 100 mg/L, the control effects of the evodiamine and the veratrole at the concentration of 300 mg/L are converged, and the indoor potting test shows that the two plant source bactericides do not generate phytotoxicity to pseudo-ginseng at different gradient concentrations, the pseudo-ginseng grows normally, the two plant source bactericides effective in controlling the pseudo-ginseng black spot disease in the Yunnan area are obtained through indoor screening, and the use safety of the plant source bactericides is further tested through the potting test, so that basic data is provided for the field test in the later period.
Claims (4)
1. Application of evodiamine or veratrole in preventing and treating black spot of Notoginseng radix caused by Alternaria alternata (ALTERNARIA ALTERNATA) is provided.
2. The use according to claim 1, wherein the bacteriostatic concentration of evodiamine is not less than 34.70 mg/L.
3. The use according to claim 1, wherein the bacteriostatic concentration of veratrole is not less than 42.99mg/L.
4. A method for preventing and treating black spot of pseudo-ginseng is characterized in that a bactericide containing evodiamine or veratrole is used and sprayed on pseudo-ginseng plants in the initial stage of disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410349331.XA CN117941700B (en) | 2024-03-26 | 2024-03-26 | Botanical fungicide for preventing and treating black spot of pseudo-ginseng |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410349331.XA CN117941700B (en) | 2024-03-26 | 2024-03-26 | Botanical fungicide for preventing and treating black spot of pseudo-ginseng |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117941700A CN117941700A (en) | 2024-04-30 |
| CN117941700B true CN117941700B (en) | 2024-06-04 |
Family
ID=90805499
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410349331.XA Active CN117941700B (en) | 2024-03-26 | 2024-03-26 | Botanical fungicide for preventing and treating black spot of pseudo-ginseng |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117941700B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660726A (en) * | 2016-03-09 | 2016-06-15 | 江苏省中国科学院植物研究所 | Preparation method and application of evodia rutaecarpa serving as agricultural fungicide |
| CN112438272A (en) * | 2020-12-03 | 2021-03-05 | 兰州大学 | Evodiamine analog and application thereof in preventing and treating plant pathogenic fungi |
| CN113354645A (en) * | 2021-06-29 | 2021-09-07 | 天津农学院 | Preparation of evodiamine and application of evodiamine in aspect of preventing and controlling agricultural pests |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140336159A1 (en) * | 2011-10-07 | 2014-11-13 | Pulmatrix, Inc. | Methods for treating and diagnosing respiratory tract infections |
| KR101923721B1 (en) * | 2016-12-06 | 2018-11-29 | 에스지상사 주식회사 | Composition for the preventing or treating of caffeinism comprising Evodia officinalis or evodiamine |
-
2024
- 2024-03-26 CN CN202410349331.XA patent/CN117941700B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660726A (en) * | 2016-03-09 | 2016-06-15 | 江苏省中国科学院植物研究所 | Preparation method and application of evodia rutaecarpa serving as agricultural fungicide |
| CN112438272A (en) * | 2020-12-03 | 2021-03-05 | 兰州大学 | Evodiamine analog and application thereof in preventing and treating plant pathogenic fungi |
| CN113354645A (en) * | 2021-06-29 | 2021-09-07 | 天津农学院 | Preparation of evodiamine and application of evodiamine in aspect of preventing and controlling agricultural pests |
Non-Patent Citations (2)
| Title |
|---|
| 吴茱萸碱、吴茱萸次碱和左旋四氢巴马亭在微生物和大鼠体内的代谢研究;李丽;中国博士学位论文全文数据库医药卫生科技辑;20090415(第4期);第E057-14页 * |
| 基于UPLC-Q-TOF-MS/MS分析藜芦中化学成分;贾晓华等;天津中医药大学学报;20210215;第40卷(第1期);第104-111页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117941700A (en) | 2024-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111763629B (en) | Bacillus belgii and application thereof | |
| CN108375658B (en) | Method for evaluating control effect of bactericide on black spot of potato seedlings | |
| CN101965848B (en) | Biogenic pesticide for efficiently preventing and controlling main diseases of Camellia oleifera and application thereof | |
| CN113661992B (en) | Application of ligustilide and plant essential oil containing ligustilide in prevention and treatment of peanut southern blight | |
| CN114668004A (en) | Application of perillaldehyde in preparation of bactericide for preventing and treating plant diseases | |
| CN109628550A (en) | A kind ofly by the screening technique of chrysanthemum anti-blight kind | |
| CN117981776A (en) | Biological control application of Murraya paniculata, biological control preparation, preparation method and biological control method thereof | |
| CN103004894A (en) | Biopesticide for preventing and treating powdery mildew of melons and fruits and preparation method of biopesticide | |
| CN117941700B (en) | Botanical fungicide for preventing and treating black spot of pseudo-ginseng | |
| CN101502275A (en) | Plant source pesticide | |
| RU2666920C1 (en) | Method for obtaining adventitious roots of phlojodicarpus sibiricus (steph.) k.-pol. under in vitro conditions | |
| CN116686834A (en) | Application of quercetin in preparation of bactericide for preventing and treating plant blight | |
| CN112106789B (en) | Application of delphinium ethyl acetate extract in preventing and treating diamond back moth | |
| CN105838613A (en) | Pigeon pea endophytic fungi high in yield of flavipin and application of pigeon pea endophytic fungi | |
| Singh et al. | Screening of potential antimicrobial activity of Indian medicinal plant of different solvent extract: Tinospora cordifolia and Hymenocallis littoralis | |
| CN105211078A (en) | A kind of compound suppressing larch pin spoting disease bacteria growing | |
| CN104542135A (en) | Method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide | |
| CN109699683A (en) | A kind of talcum matrix Java cordyceps sinensis spore preparation | |
| CN115363055B (en) | Preparation method and application of spanishneedles herb extract liquid for preventing and treating crop bacterial wilt | |
| CN112493240B (en) | Control agent for tomato bacterial wilt and preparation method thereof | |
| CN109370910A (en) | A kind of scopulariopsis brevicaulis and application thereof | |
| CN104120084B (en) | A kind of yellowish green green muscardine fungus MFYY090714 and its application | |
| CN117535201B (en) | Bacillus bailii HM-6 and application thereof | |
| CN112625920B (en) | Application of disease and insect resistant endophytic fungi | |
| CN109247347B (en) | Application of fingered citron volatile oil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |