CN101502275A - Plant source pesticide - Google Patents
Plant source pesticide Download PDFInfo
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- CN101502275A CN101502275A CNA2009101132524A CN200910113252A CN101502275A CN 101502275 A CN101502275 A CN 101502275A CN A2009101132524 A CNA2009101132524 A CN A2009101132524A CN 200910113252 A CN200910113252 A CN 200910113252A CN 101502275 A CN101502275 A CN 101502275A
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- diphtheria
- pesticide
- rhizome
- botanical pesticide
- plant pesticide
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Abstract
The present invention relates to a plant pesticide and a preparing method thereof. The plant pesticide is obtained through processing total alkaloids extracted from the root or stem of aconitum leucostomum, wherein the total alkaloids of aconitum leucostomum, water and surfactant are prepared to aqueous emulsion which can be used after diluting. The plant pesticide has better control effect to melon aphid and imported cabbageworm. The plant pesticide of the invention has the advantages of readily available raw material, low cost, safety to environment, insect disinfestation and bacteriostatic effect, suitability for compounding with other pesticide. The plant pesticide is suitable for producing non-pollution agricultural product.
Description
Technical field
The present invention relates to a kind of botanical pesticide and compound method thereof, particularly relating to a kind of is the botanical pesticide and the preparation thereof of the antibacterial and insecticidal activity of having concurrently of raw material with diphtheria rhizome of Chinese monkshood total alkaloid extract.
Background technology
For many years, chemical pesticide is efficient, quick-acting, cheap and easy to usely be widely used in worldwide because of it, is in monopoly position in plant protection.Yet, after the eighties in 20th century, along with the pay attention to day by day of masses to healthy and environmental problem, problem such as chemical pesticide is residual because of it, environmental pollution and enjoy people to query.Botanical pesticide has become one of comparatively desirable substitute products.Botanical pesticide, directly utilize or extract exactly the root, stem, leaf, flower of plant, really, seed etc. or utilize its secondary metabolites to make active substance with desinsection or bactericidal action.At present, desinsection, the sterilization idiocratic of trifoliate jewelvine, thunder godvine, Dalmatian chrysanthemum, seal chinaberry, kuh-seng, Chinese tallow tree, black nightshade, Rhododendron molle, Coriaria sinica, garlic etc. is found in succession and utilizes, wherein rotenone, pyrethrins, nimbin etc. studied comparatively successful.
The diphtheria rhizome of Chinese monkshood (Aconitum leucostomum Worosch) is a Ranunculaceae Aconitum herbaceos perennial, mainly is distributed in the Xinjiang and the northwestward, Gansu in China.In recent years, owing to reasons such as overgraze, deteriorations of grasslands, rhizome of Chinese monkshood class plant spreads on the Yi Li grassland in Xinjiang, and only the distribution area of the diphtheria rhizome of Chinese monkshood just reaches 6,000,000 mu, is local topmost poisonous plant.With the diphtheria rhizome of Chinese monkshood is the undue growth of the murder by poisoning grass of representative, and not only the health of serious harm livestock but also squeeze the growth of herbage caused heavy losses to livestock breeding production.Control to rhizome of Chinese monkshood class poisonous weeds mainly is to take manually to excavate at present, the diphtheria rhizome of Chinese monkshood of causing harm the most serious is lacked utilize.
Summary of the invention
The objective of the invention is to invent a kind of botanical pesticide, it is characterized in that: this agricultural chemicals is mainly formulated by diphtheria rhizome of Chinese monkshood total alkaloid extract interpolation non-ionic surfactant Tween 80.
Its compound method is: diphtheria rhizome of Chinese monkshood root is cleaned the back dry naturally, get the root powder behind the crushing screening with 95% ethanol cold soaking 3 times, each 48 hours; Merge leaching liquor, concentrating under reduced pressure reclaims ethanol, gained medicinal extract 2% dissolving with hydrochloric acid; Acid liquid adds concentrated ammonia liquor after with petroleum ether degreasing and alkalizes to pH9～10, and with chloroform extraction repeatedly, combining extraction liquid reclaims chloroform and gets thickness medicinal extract; With the dissolving of 0.1% the Tween 80 aqueous solution, be made into the stoste of suitable concn after the natural seasoning, preserve under the room temperature standby, can be according to actual needs during use, thin up becomes variable concentrations to use.More than operation all can at room temperature be carried out.
The invention has the advantages that: 1. environmentally safe, primary raw material derives from plant; 2. raw material is easy to get, and turns bane into boon, and processing is simple, and is low for equipment requirements; 3. cost is saved in all recyclable repeated use such as the used organic solvent of production process such as alcohol and chloroform etc.; 4. played a role jointly by multiple aconite alkaloid, insect or disease fungus are difficult for developing immunity to drugs; 5. have desinsection and bacteriostasis concurrently; 6. can be mixed reinforced effects with other biological agricultural chemicals or chemical pesticide.
Embodiment
Further illustrate content of the present invention below in conjunction with embodiment, but the present invention is not restricted to the scope of following embodiment.
Embodiment one:
Take by weighing diphtheria aconite root powder 100g, add 500ml, 400ml, 300ml95% ethanol cold soaking 3 times respectively, each 48 hours; Merge leaching liquor, concentrating under reduced pressure reclaims ethanol, gained medicinal extract 2% dissolving with hydrochloric acid; Acid liquid adds concentrated ammonia liquor after with petroleum ether degreasing and alkalizes to pH9～10, and with chloroform extraction repeatedly, combining extraction liquid reclaims chloroform and gets thickness alkaloid medicinal extract.
Embodiment two:
The Tween 80 aqueous solution with 100ml0.1% after the alkaloid medicinal extract natural seasoning among the embodiment one is dissolved, obtain the stoste of 1g/ml.
Stoste by embodiment two preparations is carried out indoor determination of activity:
Bacteriostatic activity is measured:
1. Plating suppresses the test of disease fungus mycelial growth: under aseptic condition, quantitatively drawing stoste adds in the sterilized PDA medium, pour into while hot after shaking up in the culture dish that diameter is 90mm and make flat board, be made into the pastille medium of 100mg/ml, 20mg/ml, 4mg/ml respectively, not contain the blank that is treated to of soup; Aseptic card punch with diameter 7mm cuts bacterium cake horizontalization plate central authorities, every ware a slice, and every processing repeats for 3 times, puts in 28 ℃ of incubators and cultivates; Routine observation is also measured colony diameter with the right-angled intersection method, calculates growth inhibition ratio.
2. the concave slide method suppresses the spore germination test: make it produce spore after will cultivating certain hour for the examination pathogen, scrape and get spore and be made into the spore suspension of debita spissitudo (under 10 * 10 low power lens, 40～50 spores in every visual field are advisable), quantitatively draw and mix with stoste that to make the soup final concentration be 20mg/ml, to add sterile water in the spore suspension is contrast, and every processing repeats for 3 times; Draw above-mentioned mixed liquor and drip on the concave slide, frame is put in the culture dish that has shallow seated groundwater then, and adding a cover preserves moisture is incubated in the incubator of preference temperature; Check spore germination rate (spore germ tube length is considered as sprouting greater than the spore short radius) behind 8h, the 24h.
Insecticidal activity assay (cotten aphid):
1. contact toxicity is measured: adopt and carry aphid blade infusion process.Cultivate cotton seedling in the greenhouse with nutrient solution, every glass two strain connects aptery one-tenth aphid in the every strain of cotyledon period and counts head, shrouds on the cover, treats nearly hundred of two leaf period natural propagations, rejects unnecessary aphid, only the uniform aptery one-tenth aphid of reservation size; Stoste is diluted to variable concentrations, takes out cotton seedling and in soup, dip 5s, inhale and remove unnecessary soup, the aphid number of counting immediately on the cauline leaf is insect population radix before handling, and every glass is a repetition, and every processing repeats for 3 times, with distilled water is blank, 800 times of positive contrasts of liquid of 40% flolimat; In handling back 24h, 48h, 72h record survival borer population, calculate lethality and with the Abbott formula correction.
2. antifeedant activity is measured: stoste is diluted to variable concentrations and is blank with distilled water, 800 times of positive contrasts of liquid of 40% flolimat; Choose the cotton young plant leaf of area unanimity, with middle vein be the boundary respectively handle and contrast solution in dip 5s, take out dry after, its blade back switching wingless aphid in 30 3～4 ages, cotton leaf blade back is put into culture dish down also ties up the petiole cultivation of preserving moisture with wet cotton balls; Every processing 4 times repeats, 24 and 48h after check the situation of perching of cotten aphid on the blade of respectively handling, calculate the food refusal rate.
Result of the test:
The bacteriostatic activity result
1. to the inhibitory action of hypha,hyphae growth
For reagent liquid the mycelial growths of Pyricularia oryzae, Italian mould, muskmelon wilt, melon fusarium solani, 5 kinds of pathogens of cucumber phytophthora root rot bacterium all there is certain inhibitory action, and increases inhibiting rate in time and increase, hold and imitate better.Under 100mg/ml concentration, soup all is higher than 50% to the inhibiting rate of each pathogen, and the inhibiting rate to cucumber phytophthora root rot bacterium behind the 7d reaches 87.72%, and the inhibiting rate of Pyricularia oryzae and Italian mould is also reached 70.18% and 69.68% respectively; When concentration was 4mg/ml, the inhibiting rate to each pathogen behind the 7d also all was higher than 25%, and the inhibiting rate of muskmelon wilt and cucumber phytophthora root rot bacterium is respectively 43.50% and 43.83%.
2. to the inhibitory action of fungus spore germination
Soup has had strong inhibitory effects to melon fusarium solani and cucumber phytophthora root rot bacterium under 20mg/ml concentration, and inhibiting rate is 100%, but drug effect increases in time and descends; The muskmelon wilt also there is good inhibitory effect.
Insecticidal activity result
1. to the contact toxicity of cotten aphid
For reagent liquid cotten aphid is had contact toxicity preferably, lethality improves with the increase of concentration, and the 48h lethality reaches 100% under the 200mg/ml concentration, and the 72h lethality reaches 100% under the 100mg/ml concentration.Corrected mortality and 800 times of liquid of 40% flolimat of 50mg/ml concentration prescribe medicine back 48h, 72h do not have significant difference, but drug action is slower than flolimat, and this also is the popular feature of biopesticide.But in 72h, the lethality that each concentration liquid is handled increases the LC of 24h, 48h, 72h behind the medicine gradually
50Be respectively 14.30mg/ml, 9.30mg/ml, 7.84mg/ml, show that the soup virulence constantly strengthens.
2. to the antifeedant activity of cotten aphid
100mg/ml concentration prescribe medicine back 24 and 48h food refusal rate are respectively 87.63% and 93.81%, do not have significant difference with 800 times of liquid of 40% flolimat; The food refusal rate significantly descended when concentration was 4mg/ml, less than 20%.Each concentration prescribe medicine back 48h increases than the food refusal rate of 24h, but amplification is little.Duration of test is not observed and is respectively handled soup growth has harmful effect to cotton seedling.
Claims (3)
1. botanical pesticide, by the total alkaloid extract of the diphtheria rhizome of Chinese monkshood (Aconitum leucostomum Worosch) root or stem, it is formulated to add surfactant.
2. botanical pesticide as claimed in claim 1 is characterized in that: active component is the total alkaloids in the diphtheria rhizome of Chinese monkshood.
3. the collocation method of botanical pesticide as claimed in claim 1 is characterized in that: with the diphtheria aconite root pulverized or stem powder with 95% ethanol cold soaking 3 times, each 48 hours; Merge leaching liquor, concentrating under reduced pressure reclaims ethanol, gained medicinal extract 2% dissolving with hydrochloric acid; Acid liquid adds concentrated ammonia liquor after with petroleum ether degreasing and alkalizes to pH9～10, and with chloroform extraction repeatedly, combining extraction liquid reclaims chloroform and gets thickness medicinal extract; The Tween 80 aqueous solution with 0.1% after the natural seasoning dissolves, and is made into aqueous emulsion stoste, preserves under the room temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009101132524A CN101502275A (en) | 2009-03-12 | 2009-03-12 | Plant source pesticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009101132524A CN101502275A (en) | 2009-03-12 | 2009-03-12 | Plant source pesticide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101502275A true CN101502275A (en) | 2009-08-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2009101132524A Pending CN101502275A (en) | 2009-03-12 | 2009-03-12 | Plant source pesticide |
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| CN (1) | CN101502275A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102038013A (en) * | 2010-11-29 | 2011-05-04 | 西南科技大学 | Preparation method and application of monkshood total diterpene biological pesticide |
| CN102934632A (en) * | 2012-11-20 | 2013-02-20 | 江苏省中国科学院植物研究所 | Application of yunaconitine in pesticides |
| CN104872204A (en) * | 2015-06-17 | 2015-09-02 | 山西农业大学 | Vegetable bactericide capable of preventing and treating phytophthora root rot of vegetables and preparation method of vegetable bactericide |
| CN104886184A (en) * | 2015-06-12 | 2015-09-09 | 山西农业大学 | Botanical fungicide for preventing and controlling tobacco mosaic virus and preparation method |
| CN104920125A (en) * | 2015-06-29 | 2015-09-23 | 贵州黄平靓鸥桑综合开发有限公司 | Preventing and treating method for powdery mildew of lagerstroemia indica trees |
-
2009
- 2009-03-12 CN CNA2009101132524A patent/CN101502275A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102038013A (en) * | 2010-11-29 | 2011-05-04 | 西南科技大学 | Preparation method and application of monkshood total diterpene biological pesticide |
| CN102934632A (en) * | 2012-11-20 | 2013-02-20 | 江苏省中国科学院植物研究所 | Application of yunaconitine in pesticides |
| CN102934632B (en) * | 2012-11-20 | 2014-04-09 | 江苏省中国科学院植物研究所 | Application of yunaconitine in pesticides |
| CN104886184A (en) * | 2015-06-12 | 2015-09-09 | 山西农业大学 | Botanical fungicide for preventing and controlling tobacco mosaic virus and preparation method |
| CN104886184B (en) * | 2015-06-12 | 2017-09-05 | 山西农业大学 | A kind of botanical fungicide and preparation method for preventing and treating tobacco mosaic virus disease |
| CN104872204A (en) * | 2015-06-17 | 2015-09-02 | 山西农业大学 | Vegetable bactericide capable of preventing and treating phytophthora root rot of vegetables and preparation method of vegetable bactericide |
| CN104920125A (en) * | 2015-06-29 | 2015-09-23 | 贵州黄平靓鸥桑综合开发有限公司 | Preventing and treating method for powdery mildew of lagerstroemia indica trees |
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Application publication date: 20090812 |