CN102415378A - Bactericide capable of promoting crop growth and preventing and treating pepper phytophthora blight, and use thereof - Google Patents

Bactericide capable of promoting crop growth and preventing and treating pepper phytophthora blight, and use thereof Download PDF

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Publication number
CN102415378A
CN102415378A CN2011103879799A CN201110387979A CN102415378A CN 102415378 A CN102415378 A CN 102415378A CN 2011103879799 A CN2011103879799 A CN 2011103879799A CN 201110387979 A CN201110387979 A CN 201110387979A CN 102415378 A CN102415378 A CN 102415378A
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bactericide
preventing
crop growth
phytophthora blight
alcohol
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石志琦
薛延丰
王帆
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Jiangsu Academy of Agricultural Sciences
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Jiangsu Academy of Agricultural Sciences
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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Abstract

The invention discloses a bactericide capable of promoting crop growth and preventing and treating pepper phytophthora blight, and a use thereof, and belongs to the field of pesticides for crop disease prevention and treatment. The bactericide capable of promoting crop growth and preventing and treating pepper phytophthora blight comprises: by weight, 0.1 to 10% of cinnamaldehyde, 10 to 30% of an emulsifier, 0.1 to 10% of azone, 20 to 50% of alcohol and the balance water. A preparation method of the bactericide capable of promoting crop growth and preventing and treating pepper phytophthora blight comprises that cinnamaldehyde and azone are respectively dissolved in the alcohol and are mixed well. The invention also discloses the use of the bactericide in the prevention and treatment on pepper phytophthora blight and the promotion of crop growth. The bactericide capable of promoting crop growth and preventing and treating pepper phytophthora blight is a botanical bactericide and contains active ingredients from cinnamaldehyde which is natural plant extract, and thus the multiple effects of promoting crop growth and development, increasing a yield and income, improving quality and a nutrient utilization rate, controlling insects and preventing environmental pollution are obtained. The bactericide capable of promoting crop growth and preventing and treating pepper phytophthora blight is suitable for production of nuisanceless, green and organic agricultural products. The bactericide capable of promoting crop growth and preventing and treating pepper phytophthora blight has a low cost, obvious use effects and wide application prospects.

Description

A kind of bactericide and application thereof that promotes plant growth and prevent and treat capsicum epidemic disease
Technical field
The present invention relates to the pesticide field of control of crop disease, relate in particular to a kind of bactericide and application thereof that promotes plant growth and prevent and treat capsicum epidemic disease.
Background technology
Capsicum is wide at China's planting range, area is big.According to incompletely statistics, the whole nation has 27 provinces and regions that drying pepper cultivation is arranged, and year plantation is more than 5,000,000 mu.Capsicum has become the specialty economies crop of part province peasant programme.But in recent years, because the continuous cropping of same kind, the soil of facility cultivation often is in the environment of high temperature, high humidity, high evaporation, no rainwater leaching, causes eqpidemic disease to increase the weight of year by year, and the lesion is expansion year by year from south to north.Annual because of capsicum epidemic disease causes damage 20~30%, up to 70~80%, production loss is one of severe diseases on capsicum produces very significantly when serious.Because of its pathogen time-to-live in soil long, and, endanger more and more serious along with continuous cropping germ number increases year by year.Capsicum epidemic disease all can take place in the whole growing of capsicum, especially when capsicum buddings and bear fruit, takes place serious.Under the suitable situation of weather conditions, this disease can endanger root, stem stalk, branch, fruit and blade, can cause dead seedling, decayed fruit, the underproduction even total crop failure when serious.Capsicum epidemic disease is also claimed capsicum fusarium wilt, Phytophthora capsici disease; Be a kind of soil-borne disease that causes by Phytophthora capsici (Phytophthora capsici), can be referred to as " capsicum cancer ", often cause the capsicum underproduction; Very end total crop failure, become the destructive disease of capsicum in the world wide.The capsicum epidemic disease control has become national capsicum and has produced the key subjects that face.
Over past ten years, people have carried out a series of chemical control technical research on the basis of investigating thoroughly the capsicum epidemic disease pathogen, obtained certain result, do not prevent and treat a difficult problem but fundamentally solve capsicum epidemic disease yet.The chemical control to vegetable epidemic disease at present mainly also is to use chemical pesticide, as: aliette, metalaxyl, Pu Like, Amici reach etc.But, use chemical pesticide not only can produce residue of pesticide, and along with the pesticide resistance of using phytophthora year by year of chemical pesticide is also increasing gradually; The vegetable grower can only strengthen the usage amount of agricultural chemicals for this reason, if excessively strengthen consumption, not only can influence chili growth; Increase persticide residue in the pepper fruit; Suppress beneficial microbe growth in the soil, and pollute the environment, increase production cost.The technology appearance that suppresses Phytophthora capsici through microorganism is also arranged at present; Like one Chinese patent application " a kind of preparation method and application that prevents and treats the bacillus brevis and the biologic product of capsicum epidemic disease " (application number CN102154178), and " the antagonism bacterium and the microorganism organic fertilizer thereof of control continuous cropping capsicum epidemic disease " (application number CN101948780) all discloses to adopt the inhibiting microorganism of its antagonism of Phytophthora capsici prevented and treated capsicum epidemic disease as main component.Though these methods have the antagonism inhibitory action in the experimental stage proof to Phytophthora capsici; But the control efficiency in real production application process remains to be investigated; And above biologic product costs an arm and a leg, and is unfavorable for promoting, and introduces the microbial balance that new microorganism fungus kind also possibly destroyed soil.
Summary of the invention
Goal of the invention: the purpose of this invention is to provide a kind of bactericide that promotes plant growth and prevent and treat capsicum epidemic disease, can effectively prevent and treat capsicum epidemic disease, have the effect that promotes plant growth simultaneously concurrently; A further object of the invention provides the preparation method of aforementioned bactericide; A further object of the invention provides the application of aforementioned bactericide on the control capsicum epidemic disease, and in the application that promotes in the plant growth.
Technical scheme: for a kind of bactericide that promotes plant growth and prevent and treat capsicum epidemic disease; For realizing above-mentioned purpose; Bactericide of the present invention comprises following composition according to percentage by weight: cinnamic acid 0.1~10%, emulsifier 10~30%, azone 0.1~10%; Alcohol 20~50%, all the other are water.
The present invention is main active with the cinnamic acid, and Phytophthora capsici is had good fungistatic effect, because it be native compound, the g and D of crop all is safe from harm efficiently noresidue, no pesticide resistance.Azone has another name called 1-dodecyl-aza-cycloheptane alkane-2-ketone, is the good penetration agent, can promote the infiltration of medicament to plant and pathogene, improves the effect of active component.This bactericide is an emulsion dosage form, is solvent with alcohol.
As prioritization scheme of the present invention, the said bactericide that promotes plant growth comprises following composition according to percentage by weight: cinnamic acid 1%, and emulsifier 20%, azone 2%, alcohol 40%, all the other are water.
For a kind of preparation method who promotes the bactericide of plant growth, mix the formation solution A first cinnamic acid is added in a part of alcohol, again emulsifier and azone formed solution B with all the other alcohol dissolvings, then A and B mixed, and adds the water constant volume.Because cinnamic acid and azone all are dissolved in alcohol, therefore adopt alcohol as solvent.
Crop of the present invention comprises capsicum, custard squash, cucumber, eggplant, tomato or green vegetables; The scope of application of bactericide of the present invention is wide; Not only can prevent and treat the capsicum epidemic disease of capsicum, custard squash, cucumber, eggplant, tomato, watermelon, pumpkin, potatoes and other crops, also plant growth had facilitation.
Beneficial effect: a kind of its effective component of bactericide that promotes to grow and prevent and treat capsicum epidemic disease of the present invention derives from the natural plant extracts cinnamic acid; Have promote crop growth, increasing both production and income, improve quality, improve plant recovery of nutrient, pest control, multi-efficiency such as pollution-free, be applicable to nuisanceless, green and organic farm products production.The cost of said preparation is not high, and effect is remarkable, has very wide application prospect.
Embodiment
Embodiment 1
A kind of bactericide that promotes plant growth and prevent and treat capsicum epidemic disease comprises following composition according to percentage by weight: cinnamic acid 1%, and emulsifier 20%, azone 2%, alcohol 40%, all the other are water.Mix the formation solution A first cinnamic acid is added in the alcohol, again emulsifier and azone are formed solution B with the alcohol dissolving, then A and B mixed, and carries out constant volume with pure water at last.
Embodiment 2
A kind of bactericide that promotes plant growth and prevent and treat capsicum epidemic disease comprises following composition according to percentage by weight: cinnamic acid 5%, and emulsifier 15%, azone 2%, alcohol 40%, all the other are water.Mix the formation solution A first cinnamic acid is added in the alcohol, again emulsifier and azone are formed solution B with the alcohol dissolving, then A and B mixed, and carries out constant volume with pure water at last.
Embodiment 3
A kind of bactericide that promotes plant growth and prevent and treat capsicum epidemic disease comprises following composition according to percentage by weight: cinnamic acid 0.5%, and emulsifier 20%, azone 2%, alcohol 40%, all the other are water.Mix the formation solution A first cinnamic acid is added in the alcohol, again emulsifier and azone are formed solution B with the alcohol dissolving, then A and B mixed, and carries out constant volume with pure water at last.
Embodiment 4
A kind of bactericide that promotes plant growth and prevent and treat capsicum epidemic disease comprises following composition according to percentage by weight: cinnamic acid 0.1%, and emulsifier 10%, azone 10%, alcohol 50%, all the other are water.Mix the formation solution A first cinnamic acid is added in the alcohol, again emulsifier and azone are formed solution B with the alcohol dissolving, then A and B mixed, and carries out constant volume with pure water at last.
Embodiment 5
A kind of bactericide that promotes plant growth and prevent and treat capsicum epidemic disease comprises following composition according to percentage by weight: cinnamic acid 10%, and emulsifier 30%, azone 0.1%, alcohol 20%, all the other are water.Mix the formation solution A first cinnamic acid is added in the alcohol, again emulsifier and azone are formed solution B with the alcohol dissolving, then A and B mixed, and carries out constant volume with pure water at last.
Embodiment 6
Bactericide of the present invention has superior control efficiency on capsicum epidemic disease, beyond thought facilitation is also played in the growth of crop, obtains beyond thought facilitation effect.Describe in the growth that promotes crop with regard to botanical fungicide of the present invention below.
Formula I: 1/2Hoagland nutrient solution.
Formula I I: like embodiment 1 described botanical fungicide based on cinnamic acid.
With capsicum and cucumber seeds H with 0.3% (w/w) 2O 2After the sterilization, clean, select the culture dish (diameter 15cm) that homogeneous, the normal seed of form place shop 3 metafiltration paper then, train seedling with distilled water.5d after waiting to emerge chooses the consistent material of growing way it is transferred in the plastic basin alms bowl, behind the seedling age 20d, sprays with containing different disposal liquid respectively, and each handles repetition 3 times.Treatment fluid is every to spray once at a distance from 5d, samples behind the 60d, measures each processed group relevant physiological biochemical indicator (annotate: contrast CK is a clear water).
Testing index comprises fresh weight, protein content, nitrate content, V cContent, soluble sugar content, the result is as shown in table 1.
Fresh weight is measured: after the off-test, every basin is the consistent seedling of 3 upgrowth situations of picking at random, measures its fresh weight with the clean back of blotting of deionized water then.
Protein content determination: close generation method with reference to Lee, adopt Coomassie brilliant blue method (Li Hesheng " plant physiology and biochemistry experimental principle and technology " Beijing Higher Education Publishing House, 2000).
Nitrate content is measured: with reference to methods such as Lu Qiming, measure and adopt ultraviolet spectrophotometry (Lu Qiming etc. " improvement of determined by ultraviolet spectrophotometry nitrate nitrogen on vegetables " Agricultural University Of South China's journal, 1997,8 (4): 104-106).
V cMeasure: with reference to high friend's method, get about testing sample 2g, add a small amount of 2% hydrochloric acid, in tissue mashing machine, process homogenate; Move in the 100mL volumetric flask, to scale, absorbent cotton filters with 1% oxalic acid constant volume; Get 10mL filtrating, with demarcated 2,6-dichloroindophenol solution titration determination V cContent (Gao Peng " agrochemistry common analysis " Xi'an: Shaanxi science tech publishing house, 1980).
Soluble sugar is measured: with reference to the Zou Qi method, adopt anthrone colorimetric method (Zou Qi " plant physiology experiment guidance " Beijing: Chinese agriculture publishing house, 2000).
Table 1 different disposal is to the influence of capsicum quality
Can know that by table 1 capsicum is handled back fresh weight, protein content, V in formula I cContent and soluble sugar are compared respectively according to having increased by 26.2%, 25.3%, 16.4% and 45.4%, and the nitrate content comparison is according to increasing by 5.1%; Fresh weight, protein content, V after formula I I handles cContent and soluble sugar are compared respectively according to having increased by 35.5%, 47.6%, 47.0% and 61.6%, and the nitrate content comparison is according to reducing 9.4%.On the whole, adopt bactericide of the present invention to help lend some impetus to plant growing, and effect is better than general nutrient solution.
In addition, make an experiment with regard to cucumber with the same treatment group, mensuration comprises fresh weight, protein content, nitrate content, V again cContent and soluble sugar content, the result is as shown in table 2.
Table 2 different disposal is to the influence of cucumber quality
Figure BDA0000114075910000062
Can know that by table 2 cucumber is handled back fresh weight, protein content, V in formula I cContent and soluble sugar are compared respectively according to having increased by 19.0%, 32.7%, 20.0% and 26.8%, and the nitrate content comparison is according to increasing by 12.5%; Formula I I handles back green vegetables fresh weight, protein content, Vc content and soluble sugar and compares respectively according to having increased by 35.9%, 37.8%, 28.3% and 40.4%, and the nitrate content comparison is according to reducing 3.4%.It is thus clear that bactericide of the present invention also has obvious facilitation to the growth of cucumber.
Embodiment 7
Bactericide of the present invention has good control efficiency to the crop capsicum epidemic disease, than other traditional sterilization pesticide better effects if of single employing.Describe through comparative trial with regard to cucumber and capsicum below.
Formula I: 20% carbendazol wettable powder.
Formula I I: embodiment 1 and 65% mancozeb wettable powder are composite.
Formula I II: like embodiment 1 described bactericide based on cinnamic acid.
Choose cucumber morbidity field and 3 processing of capsicum morbidity field design: handle 1: contrast (CK) is clear water; Handle 2: formula I; Handle 3: formula I I; Handle 4: formula I II.Each handles repetition 3 times, and treatment fluid is every to spray once at a distance from 5d, analyzes after company sprays 2 times.
Adopt disease index and control efficiency to measure: with reference to the method for Dou Ruimu (Dou Ruimu " the plants endogenetic bacterium is to the control efficiency of graw mold of tomato " Henan agricultural science, 2010,4:77-79).The result is as shown in table 3.
Can know that by table 3 on capsicum, formula I is 40.95% to the preventive effect of Phytophthora capsici; Formula I I is 78.61% to the preventive effect of Phytophthora capsici; Formula I II is 80.45% to the preventive effect of Phytophthora capsici.As far as cucumber, formula I is 37.15% to the mould preventive effect of epidemic disease; Formula I I is 72.06% to the mould preventive effect of epidemic disease; Formula I II is 77.03% to the mould preventive effect of epidemic disease.It is thus clear that adopt the bactericidal effect of bactericide of the present invention to be significantly higher than the employing carbendazim.
Table 3 different disposal is to the mould control efficiency of the epidemic disease of capsicum and cucumber
Figure BDA0000114075910000071
The above only is a preferred implementation of the present invention; Be noted that for those skilled in the art; Under the prerequisite that does not break away from the principle of the invention, can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.

Claims (5)

1. bactericide that can promote to grow and prevent and treat capsicum epidemic disease is characterized in that comprising following composition according to percentage by weight:
Cinnamic acid 0.1 ~ 10%,
Emulsifier 10 ~ 30%,
Azone 0.1 ~ 10%,
Alcohol 20 ~ 50%,
All the other are water.
2. a kind of bactericide that promotes to grow and prevent and treat capsicum epidemic disease according to claim 1 is characterized in that comprising following composition according to percentage by weight:
Cinnamic acid 1%,
Emulsifier 20%,
Azone 2%,
Alcohol 40%,
All the other are water.
3. the preparation method of bactericide according to claim 1 is characterized in that earlier cinnamic acid being added mixing the formation solution A in a part of alcohol, again with emulsifier and azone with all the other alcohol dissolving formation solution B, then A and B are mixed, add the water constant volume.
4. the application of bactericide on the control capsicum epidemic disease according to claim 1.
5. the application of bactericide in the promotion plant growth according to claim 1.
CN2011103879799A 2011-11-30 2011-11-30 Bactericide capable of promoting crop growth and preventing and treating pepper phytophthora blight, and use thereof Pending CN102415378A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102823591A (en) * 2012-09-19 2012-12-19 陈琳 Botanical fungicide containing cinnamaldehyde and preparation method thereof
CN105409949A (en) * 2015-11-26 2016-03-23 江苏省农业科学院 Plant source sterilization preparation based on cis-form cinnamaldehyde and preparation method and application thereof
CN105409948A (en) * 2015-11-26 2016-03-23 江苏省农业科学院 Cis-form cinnamic aldehyde plant source bactericidal agent and preparation method and application thereof

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CN101578984A (en) * 2009-01-22 2009-11-18 江苏省农业科学院 Algaecide combined by cinnamic aldehyde, eugenol and citric acid
CN101982109A (en) * 2010-09-06 2011-03-02 南昌大学 Method for preparing cinnamon oil microemulsion

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4525480A (en) * 1983-06-10 1985-06-25 Sutton Laboratories, Inc. Composition of matter containing cinnamaldehyde and parabens
CN101578984A (en) * 2009-01-22 2009-11-18 江苏省农业科学院 Algaecide combined by cinnamic aldehyde, eugenol and citric acid
CN101982109A (en) * 2010-09-06 2011-03-02 南昌大学 Method for preparing cinnamon oil microemulsion

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
《西北农林科技大学硕士论文》 20071115 王宏年 "几种植物精油抑菌作用研究" , *
王宏年: ""几种植物精油抑菌作用研究"", 《西北农林科技大学硕士论文》, 15 November 2007 (2007-11-15) *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102823591A (en) * 2012-09-19 2012-12-19 陈琳 Botanical fungicide containing cinnamaldehyde and preparation method thereof
CN105409949A (en) * 2015-11-26 2016-03-23 江苏省农业科学院 Plant source sterilization preparation based on cis-form cinnamaldehyde and preparation method and application thereof
CN105409948A (en) * 2015-11-26 2016-03-23 江苏省农业科学院 Cis-form cinnamic aldehyde plant source bactericidal agent and preparation method and application thereof

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Application publication date: 20120418