CN102125064B - Rhizospheric integrated regulation and control agent for root-knot nematodes positioned in greenhouse vegetable soil and application method thereof - Google Patents
Rhizospheric integrated regulation and control agent for root-knot nematodes positioned in greenhouse vegetable soil and application method thereof Download PDFInfo
- Publication number
- CN102125064B CN102125064B CN 201010591023 CN201010591023A CN102125064B CN 102125064 B CN102125064 B CN 102125064B CN 201010591023 CN201010591023 CN 201010591023 CN 201010591023 A CN201010591023 A CN 201010591023A CN 102125064 B CN102125064 B CN 102125064B
- Authority
- CN
- China
- Prior art keywords
- root
- rhizosphere
- agent
- comprehensive regulation
- rhizospheric
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a rhizospheric integrated regulation and control agent for root-knot nematodes positioned in a greenhouse vegetable soil and an application method thereof. The rhizospheric integrated regulation and control agent is prepared by uniformly mixing the following materials in percentage by weight: 20%-30% of garlic extracts, 50%-60% of marine green alga extracts, 4%-6% of abamectin and 8%-12% of silicic acids. The rhizospheric integrated regulation and control agent makes up the shortage of biological control effect and the limit of application range, also overcomes the defects of severe pollution of chemical control and has the advantages of good control efficiency of the root-knot nematodes, convenience for use, extensive application and little environmental pollution.
Description
Technical field
The present invention relates to the horticultural soil biological technical field, particularly a kind of facility vegetable plot root-knot nematode rhizosphere comprehensive regulation agent and application method thereof.
Background technology
In recent years, under market economy promoted, the facility cultivation industry of China demonstrated the advantage of himself gradually, and high speed development has appearred in the facility cultivation industry.Industrialized agriculture take gardening plant facility cultivations such as vegetable melon and fruits as characteristic becomes one of leading industry of Chinese agriculture gradually, becomes the important channel that Agricultural Structure Adjustment in China and vast farmers production are got rich.China's facility cultivation vegetables area reached 3,400,000 hm in 2007
2, occupy first place in the world.But because problems such as damage by disease and insect, the development of facility cultivation vegetables industry has been subject to serious restriction, and wherein the most serious parasitosis evil is the vegetables nematodiasises.
Root knot nematode disease is called again " tumor is sick ", after vegetables are encroached on, not only directly quality is grown, is reduced in impact, and production loss is serious, the general field underproduction 20%～50%, serious even total crop failure, the annual loss that causes because of root-knot nematode harm is nearly 1 000 hundred million dollars in the world.
It is predicted, following 10 years root-knot nematodes have an obvious rising, annual amplification 1% ~ 9%, meloidogyne accumulation harm will be more day by day serious under the long-term continuous cropping, and root knot nematode disease will still be one of Major Diseases of crops within following considerable time.
For meloidogyne, mainly contain at present the following method of preventing and treating: 1, cultural control, mainly adopt the methods such as soil organic improving, crop rotation, breeding for disease resistance, adjusting soil pH value.2, physical control adopts mainly that soil tans by the sun, the method such as waterflooding, steam sterilization, electrical heating, DC current sterilization, strong acidic electrolyzed water sterilization, Ozone Water sterilization, ray or ultrasonic processing.3, chemical control mainly is the agricultural chemicals of using various desinsections.4, biological control utilizes the natural enemy of root-knot nematode to carry out biological control.The cultural control technology is in theory very effective, but has serious drawback in actual production.Physical control does not have environmental pollution, people and animals without poisoning, is not reused and can develop immunity to drugs, but expend hugely, and the peasant generally is difficult to accept; Chemical control occupies critical role in nematoda control, but its side effect that brings is also apparent, such as at present used artificial synthetic nematocide, is nonspecific to the toxic action of Plant nematode, and toxicity is higher, very easily causes the harm to people and other non-target organisms; Biological control has stable, economic, long-acting and comparatively safe characteristics, can also remove the evil simultaneously volume increase, pollution abatement, preserve the ecological environment, but effect does not have chemical control fast.
In view of the characteristics of root-knot nematode, when selecting to prevent and treat method, should consider the integrated application of various Prevention Technique.
Summary of the invention
The inventor is through a large amount of Test And Research Work, finally search out a kind of effective, easy to use to the control meloidogyne, be widely used and control technique and measure that environmental pollution is little.
The object of the present invention is to provide the root-knot nematode rhizosphere comprehensive regulation agent of a kind of facility vegetable plot
And application method.
Root-knot nematode rhizosphere comprehensive regulation of the present invention agent is achieved through the following technical solutions:
1) fresh garlic is ground into the end, mixes with ethanol by quality 1:0.7～1.2, sieve
Remove residue, obtain garlic extract.
2) marine green algae with drying is ground into the end, mixes with ethanol by quality 1:0.7～1.5, and the residue that sieves obtains the marine green algae extract.
3) get garlic extract 20～30%, marine green algae extract 50～60%, Avermectin 5～10% and silicic acid 8～12% by quality, mix, make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
In order to obtain better effect, during the preparation garlic extract, preferred fresh garlic powder with
The mass ratio of ethanol is 1:0.8～1.0.During preparation marine green algae extract, the mass ratio of preferred marine green algae powder and ethanol is 1:0.8～1.0.
Root-knot nematode rhizosphere comprehensive regulation of the present invention agent can be used for fruit and vegetable, the melon vegetable kinds such as greenhouse tomato, cucumber and balsam pear; Get adjusting control agent during use and fill with the root use according to every mu 400-1000mL dosage.
Can use by the following method: before field planting 4-5 days, press using dosage 400mL pouring once for every mu.A week according to dosage 400mL pouring once after the field planting.After field planting 30-40 days, according to the pouring of dosage 800mL-1000mL stoste once.Still very serious such as deuterogenesis's situation, can water again once according to dosage 800mL-1000mL; Not not serious can not watering.
Advantage and disadvantage of the present invention is:
1, the present invention has overcome the with serious pollution defective of chemical control in the deficiency that has remedied the biological control effect and scope of application restriction.
2, the present invention is effective to root knot nematode control.
3, easy to use, be widely used, environmental pollution is little.
Description of drawings
Height growth situation after Fig. 1 tomato seedling transplanting dispenser
Fig. 2 tomato seedling is transplanted the long-living long situation of dispenser posterior lobe
Fig. 3 tomato seedling is transplanted the wide growing state of dispenser posterior lobe
Fig. 4 tomato seedling is transplanted the dispenser posterior division and is counted growing state
Fig. 5 tomato seedling is transplanted dispenser nematode population control situation
Embodiment
In order to understand better the present invention, be described further below by embodiment, but do not limit the present invention.
Embodiment 1
Avermectin: be commercially available liquid, concentration 2%;
Ethanol: be commercially available 95%(volume ratio) alcohol
Silicic acid: be commercially available solid.
Fresh garlic is ground into the end, gets 1 kilogram of 95% alcohol with 1 liter and fully mix, cross 60 mesh sieves, remove residue, get garlic extract.The marine green algae of drying is ground into the end, gets 1 kilogram of 95% alcohol with 1 liter and fully mix, cross 60 mesh sieves, remove residue, get the marine green algae extract.
Get garlic extract 35mL, marine green algae extract 55mL, Avermectin 10mL, mix, make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
Embodiment 2
Get embodiment 1 gained garlic extract 30mL and marine green algae extract 60mL, Avermectin 10mL, silicic acid 10 g mix, and make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
Get embodiment 1 gained garlic extract 25mL and marine green algae extract 60mL, Avermectin 5mL, silicic acid 10 g mix, and make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
Get embodiment 1 gained garlic extract 20mL and marine green algae extract 60mL, Avermectin 10mL, silicic acid 10 g mix, and make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
Get embodiment 1 gained garlic extract 30mL and marine green algae extract 50mL, Avermectin 10mL, silicic acid 10 g mix, and make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
Residential quarter, experimental example field pot experiment
1. test plant: tomato (jewellery kind)
2. testing program
2.1 confession examination material reaches the processing method to potted tomato
CK1: contrast (not adding any inorganic agent);
P1: Avermectin;
P2: garlic (1:1) extract+marine green algae extract;
P3: be embodiment 1 gained rhizosphere comprehensive regulation agent;
P4: embodiment 1 gained rhizosphere comprehensive regulation agent+crowndaisy chrysanthemum;
P5: be embodiment 3 gained rhizosphere comprehensive regulation agent;
P6: embodiment 3 gained rhizosphere comprehensive regulation agent+crowndaisy chrysanthemums.
Every processing repeats 3 times, 1 strain/basin, and as planting soil, every basin soil weighs 4 kg with original soil in the canopy (disease soil), and every basin is executed the fertilizer of nitrogen phosphorus potassium and is measured 1.32 g.
2.2 kind is planted, application method
In original soil in the canopy (disease soil) 21 flowerpots of packing into (common plastic flowerpot), 4 kg/ basins; Then transplant the tomato seedling (plant height 10-15cm) of a wheat harvesting period, water, dispenser, and transplant after the 30th d and 60 d totally 3 dispensers; Wherein each processes 800 times of dilutions, and 500 mL are filled with in every strain; Carry out Routine Management according to the management method of vegetables whole vegetative period.
2.3 pilot survey project and method
Plant plant height, the mensuration that stem is thick, blade is big or small: wherein plant height refers to the highest point from the root of ground plant to the top leaf; Stem slightly is the centre position between the 3rd layer and the 4th layer.Measure and make marks at the 4th layer of leaf of choosing mean size.
2.4 the collecting method of soil sample
30 d and 60 d gather the soil sample of 3 points with the crop rhizosperic at three places after in before earth boring auger is respectively in six furrow of booth after transplanting, fully behind the mixing, carry out label, and it is for subsequent use to put into refrigerator.Potted plant soil sample is to gather soil sample with earth boring auger at the flowerpot crop rhizosperic, behind the mixing, carries out label, and it is for subsequent use to put into refrigerator.
2.5 soil sample is analyzed the root-knot nematode quantitative approach
The soil sample of weighing 20 g is placed 24 h with the two-layer funnel of putting into 50 mL that binds up with gauze behind the adding distilled water.Opening spring-clip pipettes the water sample of about 5 mL in bottom to centrifuge tube.Then centrifugal 3-4 min abandons supernatant to 1 mL in the centrifuge of 2000 r/min, repeats 2 times.Get 2 after the nematode solution of handling well shaken up and be placed on respectively two places on the slide, build gently with 17mm * 17mm cover glass, be placed on the quantity of random observation nematode under 10 times of object lens, observe whole slide, convert out the nematode population of every gram soil.
3. result treatment and analysis
3.1 the impact on the potted tomato plant height
As can be seen from Figure 1, Avermectin has the harm that suppresses growth to the tomato plant height, and rhizosphere comprehensive regulation of the present invention agent has better promotion growth to the tomato plant height.
3.2 the impact long on the potted tomato leaf
As can be seen from Figure 2, Avermectin and comprehensive adjustment agent of the present invention all have the effect that promotes growth to tomato leaf length, but rhizosphere comprehensive regulation of the present invention agent is best to the long promotion growth of tomato leaf.
3.3 the impact wide on the potted tomato leaf
As can be seen from Figure 3, Avermectin and comprehensive adjustment agent of the present invention all have the effect that promotes growth to the tomato leaf is wide, but rhizosphere comprehensive regulation of the present invention agent is best to the wide promotion growth of tomato leaf.
3.4 the impact on the potted tomato branch amount
As can be seen from Figure 4, Avermectin and rhizosphere comprehensive regulation of the present invention agent all have the effect that promotes growth to the tomato branch amount, but rhizosphere comprehensive regulation of the present invention agent is better to the promotion growth to the tomato branch amount.
3.5 meloidogyne situation
As can be seen from Figure 5, the adjusting control agents such as Avermectin have certain preventive and therapeutic effect to nematode, but rhizosphere comprehensive regulation of the present invention agent has significantly extremely nematode and inhibitory action to the nematode number in the soil.In potted plant rhizosphere comprehensive regulation agent is processed the root-knot nematode of P5 to count inhibiting rate the strongest, reach 75% at twice rear inhibiting rate of dispenser, contrast CK1 and exceed 119.9 percentage points.
In addition, it can also be seen that the nematode number of 60 d CK1 check plots significantly increases after transplanting, 1 g soil sample center line borer population is near 600, and this proliferative speed that further specifies root-knot nematode is very fast, and this also is to cause one of serious major reason of root-knot nematode harm.
Claims (4)
1. facility vegetable plot root-knot nematode rhizosphere comprehensive regulation agent is characterized in that preparing by the following method:
1) fresh garlic is ground into the end, mixes with ethanol by quality 1:0.7～1.2, the residue that sieves obtains garlic extract;
2) marine green algae with drying is ground into the end, mixes with ethanol by quality 1:0.7～1.5, and the residue that sieves obtains the marine green algae extract;
3) get garlic extract 20～30%, marine green algae extract 50～60%, Avermectin 5～10% and silicic acid 8～12% by quality, mix, make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
2. according to claim 1 described a kind of facility vegetable plot root-knot nematode rhizosphere comprehensive regulation agent, when it is characterized in that preparing garlic extract, the mass ratio of fresh garlic powder and ethanol is 1:0.8～1.0.
3. according to claim 1 described a kind of facility vegetable plot root-knot nematode rhizosphere comprehensive regulation agent, when it is characterized in that preparing the marine green algae extract, the mass ratio of marine green algae powder and ethanol is 1:0.8～1.0.
4. using method such as claim 1,2 or 3 described facility vegetable plot root-knot nematode rhizosphere comprehensive regulation agent is characterized in that: 4-5 days the time, press using dosage 400mL pouring once for every mu before field planting; In every mu of when week, press using dosage 400mL and waters once after the field planting; Field planting was pressed using dosage 800mL-1000mL pouring once for every mu in the time of 30～40 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010591023 CN102125064B (en) | 2010-12-16 | 2010-12-16 | Rhizospheric integrated regulation and control agent for root-knot nematodes positioned in greenhouse vegetable soil and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010591023 CN102125064B (en) | 2010-12-16 | 2010-12-16 | Rhizospheric integrated regulation and control agent for root-knot nematodes positioned in greenhouse vegetable soil and application method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102125064A CN102125064A (en) | 2011-07-20 |
| CN102125064B true CN102125064B (en) | 2013-04-17 |
Family
ID=44263521
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010591023 Expired - Fee Related CN102125064B (en) | 2010-12-16 | 2010-12-16 | Rhizospheric integrated regulation and control agent for root-knot nematodes positioned in greenhouse vegetable soil and application method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102125064B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014147199A1 (en) * | 2013-03-20 | 2014-09-25 | Bioatlantis Ltd | A non-nematicidal composition and use thereof |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548882A (en) * | 2013-10-23 | 2014-02-05 | 鞠志国 | Biological nematicide and preparation method thereof |
| CN103918731B (en) * | 2014-04-14 | 2015-11-18 | 山东圣鹏科技股份有限公司 | A kind of composition pesticide killing plant root-knot nematode |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101193555A (en) * | 2005-04-09 | 2008-06-04 | 埃科斯普雷有限公司 | Pesticides and Repellents |
| CN101287372A (en) * | 2005-10-14 | 2008-10-15 | 吉万奥丹股份有限公司 | Pesticidal composition |
| CN101755833A (en) * | 2009-10-29 | 2010-06-30 | 深圳诺普信农化股份有限公司 | Tefluthrin-containing combination |
-
2010
- 2010-12-16 CN CN 201010591023 patent/CN102125064B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101193555A (en) * | 2005-04-09 | 2008-06-04 | 埃科斯普雷有限公司 | Pesticides and Repellents |
| CN101287372A (en) * | 2005-10-14 | 2008-10-15 | 吉万奥丹股份有限公司 | Pesticidal composition |
| CN101755833A (en) * | 2009-10-29 | 2010-06-30 | 深圳诺普信农化股份有限公司 | Tefluthrin-containing combination |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014147199A1 (en) * | 2013-03-20 | 2014-09-25 | Bioatlantis Ltd | A non-nematicidal composition and use thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102125064A (en) | 2011-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ali et al. | The effect of Ascophyllum nodosum extract on the growth, yield and fruit quality of tomato grown under tropical conditions | |
| CN104823626B (en) | A kind of prevention and control citrus yellow shoot disease harvests the Agro-ecology method of Organic Citrus simultaneously | |
| CN102515927A (en) | Drug effect type microbial fertilizer, preparation method thereof and application thereof | |
| Blok et al. | The role of autotoxins from root residues of the previous crop in the replant disease of asparagus | |
| Kayode et al. | The effects of used engine oil pollution on the growth and early seedling performance of Vigna uniguiculata and Zea mays | |
| CN107164262A (en) | A kind of microbial bacterial agent and its bacterial manure available for controlling crop diseases and insect pests | |
| CN105052643A (en) | Organic rice cultivation method | |
| CN103814799B (en) | A kind of method improveing soil | |
| CN102125064B (en) | Rhizospheric integrated regulation and control agent for root-knot nematodes positioned in greenhouse vegetable soil and application method thereof | |
| CN103798044B (en) | Stem nematode disease resistance identification method for sweet potato related wild species | |
| Vinodhini et al. | Effect of plant extracts on root-knot nematode meloidogyne incognita infecting tomato | |
| CN105475300A (en) | Compound missible oil containing oxadiazon and simetryne and preparation method thereof | |
| CN107232019A (en) | Grape stable yields is realized using miscegenation and significantly reduces the implantation methods of its cadmium content | |
| Everett et al. | Propagation of Nevada shrubs by stem cuttings. | |
| CN102415378A (en) | Bactericide capable of promoting crop growth and preventing and treating pepper phytophthora blight, and use thereof | |
| CN103719115B (en) | A kind of Pesticidal combination | |
| CN113767918A (en) | Method for preventing and treating taro rotten taro skin disease | |
| Antonov et al. | Application of a vermicomposter containing biostimulant for pine tapping | |
| De Souza et al. | Agricultural potential of reject brine from water desalination | |
| CN100401898C (en) | Composite fungus agent for preventing rot knot nematode disease of vegetable in greenhouse | |
| CN115228921B (en) | Method for repairing light and medium cadmium-polluted paddy field soil by utilizing polygonum hydropiper | |
| Kulawardhana et al. | Biopesticidal activity of Lunumidella (Melia dubia) leaf extract | |
| De Cara et al. | Inoculum sources and preservation in soils of Phytophthora parasitica from cherry tomato in continental crop areas in Southeast Spain | |
| CN102397384B (en) | Heartleaf houttuynia herb eye drops and preparation method thereof | |
| Pahlow et al. | Assessment of measures to reduce the water Footprint of cotton farming in india |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130417 Termination date: 20141216 |
|
| EXPY | Termination of patent right or utility model |