CN102125064A - Rhizospheric integrated regulation and control agent for root-knot nematodes positioned in greenhouse vegetable soil and application method thereof - Google Patents
Rhizospheric integrated regulation and control agent for root-knot nematodes positioned in greenhouse vegetable soil and application method thereof Download PDFInfo
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- CN102125064A CN102125064A CN2010105910236A CN201010591023A CN102125064A CN 102125064 A CN102125064 A CN 102125064A CN 2010105910236 A CN2010105910236 A CN 2010105910236A CN 201010591023 A CN201010591023 A CN 201010591023A CN 102125064 A CN102125064 A CN 102125064A
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Abstract
The invention discloses a rhizospheric integrated regulation and control agent for root-knot nematodes positioned in a greenhouse vegetable soil and an application method thereof. The rhizospheric integrated regulation and control agent is prepared by uniformly mixing the following materials in percentage by weight: 20%-30% of garlic extracts, 50%-60% of marine green alga extracts, 4%-6% of abamectin and 8%-12% of silicic acids. The rhizospheric integrated regulation and control agent makes up the shortage of biological control effect and the limit of application range, also overcomes the defects of severe pollution of chemical control and has the advantages of good control efficiency of the root-knot nematodes, convenience for use, extensive application and little environmental pollution.
Description
Technical field
The present invention relates to the horticultural soil biological technical field, root-knot nematode rhizosphere comprehensive regulation agent of particularly a kind of facility vegetable plot and application method thereof.
Background technology
In recent years, under market economy promoted, the facility cultivation industry of China demonstrated the advantage of himself gradually, and high speed development has appearred in the facility cultivation industry.With gardening plant facility cultivations such as vegetable melon and fruits is the industrialized agriculture of characteristic, becomes one of leading industry of Chinese agriculture gradually, becomes the important channel that Agricultural Structure Adjustment in China and vast farmers production are got rich.China's facility cultivation vegetables area reached 3,400,000 hm in 2007
2, occupy first place in the world.But because problems such as damage by disease and insect, the development of facility cultivation vegetables industry has been subjected to serious restriction, and wherein the most serious parasitosis evil is the vegetables nematodiasises.
Root knot nematode disease is called " tumor disease " again, after vegetables are encroached on, not only directly quality is grown, is reduced in influence, and production loss is serious, the general field piece underproduction 20%～50%, serious even total crop failure, nearly 1 000 hundred million dollars of the annual in the world losses that causes because of root-knot nematode harm.
It is predicted, following 10 the end of the year tie lines worm have a tangible rising, annual amplification 1% ~ 9%, soil root-knot nematode accumulation harm will be serious more day by day under the long-term continuous cropping, and root knot nematode disease will still be one of main disease of crops in following considerable time.
For the soil root-knot nematode, mainly contain the following method of preventing and treating at present: 1, cultural control, mainly adopt methods such as soil organic improving, crop rotation, breeding for disease resistance, adjusting soil pH value.2, physical control adopts mainly that soil tans by the sun, method such as waterflooding, steam sterilization, electrical heating, DC current sterilization, strong acidic electrolyzed water sterilization, Ozone Water sterilization, ray or ultrasonic processing.3, chemical control mainly is an agricultural chemicals of using various desinsections.4, biological control utilizes the natural enemy of root-knot nematode to carry out biological control.The cultural control technology is in theory very effective, but has serious drawback in actual production.Physical control does not have environmental pollution, and people and animals are not had poisoning, reuses can not develop immunity to drugs, but expends hugely, and the peasant generally is difficult to accept; Chemical control occupies critical role in nematoda control, but its side effect that brings is also apparent, as present used synthetic nematocide, is nonspecific to the toxic action of Plant nematode, and toxicity is higher, very easily causes the harm to people and other non-target organisms; Biological control has stable, economic, long-acting and comparatively safe characteristics, can also remove the evil simultaneously volume increase, pollution abatement, preserve the ecological environment, but effect does not have chemical control fast.
In view of the characteristics of root-knot nematode, when selecting to prevent and treat method, should consider the integrated application of various Prevention Technique.
Summary of the invention
The inventor is through great deal of experimental work, search out finally a kind of effective, easy to use to control soil root-knot nematode, be widely used and control technique and measure that environmental pollution is little.
The object of the present invention is to provide the root-knot nematode rhizosphere comprehensive regulation agent of a kind of facility vegetable plot
And application method.
Root-knot nematode rhizosphere comprehensive regulation of the present invention agent is achieved through the following technical solutions:
1) fresh garlic is ground into the end, mixes with ethanol, sieve by quality 1:0.7～1.2
Remove residue, obtain garlic extract.
2) marine green algae with drying is ground into the end, mixes with ethanol by quality 1:0.7～1.5, and the residue that sieves obtains the marine green algae extract.
3) get garlic extract 20～30%, marine green algae extract 50～60%, Avermectin 5～10% and silicic acid 8～12% by quality, mix, make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
In order to obtain better effect, during the preparation garlic extract, preferred fresh garlic powder with
The mass ratio of ethanol is 1:0.8～1.0.During preparation marine green algae extract, the mass ratio of preferred marine green algae powder and ethanol is 1:0.8～1.0.
Root-knot nematode rhizosphere comprehensive regulation of the present invention agent can be used for fruit and vegetable, melon vegetable kinds such as greenhouse tomato, cucumber and balsam pear; Get adjusting control agent during use and irritate the root use according to every mu 400-1000mL dosage.
Can use according to following method: before field planting 4-5 days, press using dosage 400mL for every mu and water once.A week according to dosage 400mL pouring once after the field planting.After field planting 30-40 days, once according to the pouring of dosage 800mL-1000mL stoste.Still very serious as deuterogenesis's situation, can water again once according to dosage 800mL-1000mL; Not not serious can not watering.
Advantage of the present invention and characteristics are:
1, the present invention has overcome the with serious pollution defective of chemical control in deficiency that has remedied the biological control effect and scope of application restriction.
2, the present invention is good to the root-knot nematode control efficiency.
3, easy to use, be widely used, environmental pollution is little.
Description of drawings
Plant height growing state after Fig. 1 tomato seedling transplanting dispenser
Fig. 2 tomato seedling is transplanted the long-living long situation of dispenser posterior lobe
Fig. 3 tomato seedling is transplanted the wide growing state of dispenser posterior lobe
Fig. 4 tomato seedling is transplanted the dispenser posterior division and is counted growing state
Fig. 5 tomato seedling is transplanted dispenser nematode population control situation
Embodiment
In order to understand the present invention better, be described further below by embodiment, but do not limit the present invention.
Avermectin: be commercially available liquid, concentration 2%;
Ethanol: be commercially available 95%(volume ratio) alcohol
Silicic acid: be commercially available solid.
Fresh garlic is ground into the end, gets 1 kilogram of 95% alcohol and fully mix, cross 60 mesh sieves, remove residue, get garlic extract with 1 liter.The marine green algae of drying is ground into the end, gets 1 kilogram of 95% alcohol and fully mix, cross 60 mesh sieves, remove residue, get the marine green algae extract with 1 liter.
Get garlic extract 35mL, marine green algae extract 55mL, Avermectin 10mL, mix, make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
Get embodiment 1 gained garlic extract 30mL and marine green algae extract 60mL, Avermectin 10mL, silicic acid 10 g mix, and make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
Get embodiment 1 gained garlic extract 25mL and marine green algae extract 60mL, Avermectin 5mL, silicic acid 10 g mix, and make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
Get embodiment 1 gained garlic extract 20mL and marine green algae extract 60mL, Avermectin 10mL, silicic acid 10 g mix, and make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
Get embodiment 1 gained garlic extract 30mL and marine green algae extract 50mL, Avermectin 10mL, silicic acid 10 g mix, and make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
Sub-district, experimental example field pot experiment
1. test plant: tomato (jewellery kind)
2. testing program
2.1 confession examination material reaches the processing method to potted plant tomato
CK1: contrast (not adding any inorganic agent);
P1: Avermectin;
P2: garlic (1:1) extract+marine green algae extract;
P3: be embodiment 1 gained rhizosphere comprehensive regulation agent;
P4: embodiment 1 gained rhizosphere comprehensive regulation agent+crowndaisy chrysanthemum;
P5: be embodiment 3 gained rhizosphere comprehensive regulation agent;
P6: embodiment 3 gained rhizosphere comprehensive regulation agent+crowndaisy chrysanthemums.
Every processing repeats 3 times, 1 strain/basin, and as planting soil, every basin soil weighs 4 kg with original soil (disease soil) in the canopy, and every basin is executed the fertilizer of nitrogen phosphorus potassium and is measured 1.32 g.
2.2 kind is planted, application method
Original soil in the canopy (disease soil) is packed in 21 flowerpots (common plastic flowerpot) into 4 kg/ basins; Transplant the tomato seedling (plant height 10-15cm) of a wheat harvesting period then, water, dispenser, and transplant back the 30th d and 60 d totally 3 dispensers; Wherein each handles 800 times of dilutions, and 500 mL are irritated in every strain; The whole growth phase is carried out Routine Management according to the management method of vegetables.
2.3 pilot survey project and method
Plant plant height, the mensuration that stem is thick, blade is big or small: wherein plant height refers to the highest point from the root of ground plant to the top leaf; Stem slightly is the centre position between the 3rd layer and the 4th layer.Measure and make marks at the 4th layer of leaf of choosing mean size.
2.4 the collecting method of soil sample
The soil sample that 30 d and 60 d gather 3 points with the crop rhizosperic at three places, middle back before earth boring auger is respectively in six furrow of booth after transplanting fully behind the mixing, is carried out label, and it is standby to put into refrigerator.Potted plant soil sample is to gather soil sample with earth boring auger at the flowerpot crop rhizosperic, behind the mixing, carries out label, and it is standby to put into refrigerator.
2.5 soil sample is analyzed the root-knot nematode quantitative approach
The soil sample of weighing 20 g is placed 24 h with the two-layer funnel of putting into 50 mL that binds up with gauze behind the adding distilled water.Open spring clip and pipette the water sample of about 5 mL in bottom to centrifuge tube.Centrifugal 3-4 min abandons supernatant to 1 mL then in the centrifuge of 2000 r/min, repeats 2 times.Get 2 after the nematode solution of handling well shaken up and be placed on two places on the slide respectively, build gently, be placed on the quantity of random observation nematode under 10 times of object lens, observe whole slide, convert out the nematode population of every gram soil with 17mm * 17mm cover glass.
3. result treatment and analysis
3.1 influence to potted plant tomato plant height
As can be seen from Figure 1, Avermectin has the harm that suppresses growth to the tomato plant height, and rhizosphere comprehensive regulation of the present invention agent has preferable promotion growth effect to the tomato plant height.
3.2 the influence long to potted plant tomato leaf
As can be seen from Figure 2, Avermectin and comprehensive adjustment agent of the present invention all have the effect that promotes growth to tomato leaf length, but rhizosphere comprehensive regulation of the present invention agent promotion growth effect the best long to tomato leaf.
3.3 the influence wide to potted plant tomato leaf
As can be seen from Figure 3, Avermectin and comprehensive adjustment agent of the present invention all have the effect that promotes growth to tomato leaf is wide, but rhizosphere comprehensive regulation of the present invention agent promotion growth effect the best wide to tomato leaf.
3.4 influence to potted plant tomato branch amount
As can be seen from Figure 4, Avermectin and rhizosphere comprehensive regulation of the present invention agent all have the effect that promotes growth to the tomato branch amount, but rhizosphere comprehensive regulation of the present invention agent is preferable to the promotion growth effect to the tomato branch amount.
3.5 soil root-knot nematode situation
As can be seen from Figure 5, adjusting control agents such as Avermectin have certain preventive and therapeutic effect to nematode, but rhizosphere comprehensive regulation of the present invention agent has tangible nematicide and inhibitory action to the nematode number in the soil.In potted plant rhizosphere comprehensive regulation agent is handled the root-knot nematode of P5 to count inhibiting rate the strongest, inhibiting rate reaches 75% in twice back of dispenser, contrasts CK1 and exceeds 119.9 percentage points.
In addition, it can also be seen that the nematode number of transplanting 60 d CK1 check plots, back significantly increases, 1 g soil sample center line borer population is near 600, and this proliferative speed that further specifies root-knot nematode is very fast, and this also is to cause one of serious major reason of root-knot nematode harm.
Claims (4)
1. facility vegetable plot root-knot nematode rhizosphere comprehensive regulation agent is characterized in that preparing by the following method:
1) fresh garlic is ground into the end, mixes with ethanol, sieve by quality 1:0.7～1.2
Remove residue, obtain garlic extract;
2) marine green algae with drying is ground into the end, mixes with ethanol by quality 1:0.7～1.5, and the residue that sieves obtains the marine green algae extract;
3) get garlic extract 20～30%, marine green algae extract 50～60%, Avermectin 5～10% and silicic acid 8～12% by quality, mix, make homogeneous solution, get the agent of rhizosphere comprehensive regulation.
2. according to the root-knot nematode rhizosphere comprehensive regulation agent of the described a kind of facility of claim 1 vegetable plot, when it is characterized in that preparing garlic extract, the mass ratio of fresh garlic powder and ethanol is 1:0.8～1.0.
3. according to the root-knot nematode rhizosphere comprehensive regulation agent of the described a kind of facility of claim 1 vegetable plot, when it is characterized in that preparing the marine green algae extract, the mass ratio of marine green algae powder and ethanol is 1:0.8～1.0.
4. the using method of a facility vegetable plot root-knot nematode rhizosphere comprehensive regulation agent is characterized in that: 4-5 days the time, press using dosage 400mL pouring once for every mu before field planting; In every mu of when week, press using dosage 400mL and waters once after the field planting; Field planting was pressed using dosage 800mL-1000mL pouring once for every mu in the time of 30～40 days.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548882A (en) * | 2013-10-23 | 2014-02-05 | 鞠志国 | Biological nematicide and preparation method thereof |
| CN103918731A (en) * | 2014-04-14 | 2014-07-16 | 山东圣鹏科技股份有限公司 | Pesticide composition for killing plant root-knot nematode |
| EP3295795A1 (en) * | 2013-03-20 | 2018-03-21 | Bioatlantis Limited | Use of a non-nematicidal composition |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101193555A (en) * | 2005-04-09 | 2008-06-04 | 埃科斯普雷有限公司 | Pesticides and Repellents |
| CN101287372A (en) * | 2005-10-14 | 2008-10-15 | 吉万奥丹股份有限公司 | Pesticidal composition |
| CN101755833A (en) * | 2009-10-29 | 2010-06-30 | 深圳诺普信农化股份有限公司 | Tefluthrin-containing combination |
-
2010
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101193555A (en) * | 2005-04-09 | 2008-06-04 | 埃科斯普雷有限公司 | Pesticides and Repellents |
| CN101287372A (en) * | 2005-10-14 | 2008-10-15 | 吉万奥丹股份有限公司 | Pesticidal composition |
| CN101755833A (en) * | 2009-10-29 | 2010-06-30 | 深圳诺普信农化股份有限公司 | Tefluthrin-containing combination |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3295795A1 (en) * | 2013-03-20 | 2018-03-21 | Bioatlantis Limited | Use of a non-nematicidal composition |
| US10433549B2 (en) | 2013-03-20 | 2019-10-08 | Bioatlantis Limited | Non-nematicidal composition and use thereof |
| US11582969B2 (en) | 2013-03-20 | 2023-02-21 | Bioatlantis Limited | Non-nematicidal composition and use thereof |
| CN103548882A (en) * | 2013-10-23 | 2014-02-05 | 鞠志国 | Biological nematicide and preparation method thereof |
| CN103918731A (en) * | 2014-04-14 | 2014-07-16 | 山东圣鹏科技股份有限公司 | Pesticide composition for killing plant root-knot nematode |
| CN103918731B (en) * | 2014-04-14 | 2015-11-18 | 山东圣鹏科技股份有限公司 | A kind of composition pesticide killing plant root-knot nematode |
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