CN101263813B - Application of oroxylin A as biological pesticide in preventing and controlling corps diseases - Google Patents
Application of oroxylin A as biological pesticide in preventing and controlling corps diseases Download PDFInfo
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- CN101263813B CN101263813B CN2008100472153A CN200810047215A CN101263813B CN 101263813 B CN101263813 B CN 101263813B CN 2008100472153 A CN2008100472153 A CN 2008100472153A CN 200810047215 A CN200810047215 A CN 200810047215A CN 101263813 B CN101263813 B CN 101263813B
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Abstract
The invention relates to the application of biological pesticide using Oroxylin A as the active ingredient in crop disease control; the Oroxylin A is 5, 7-dihydroxy-6-methoxyflavone, which is a yellow needle like crystal with the melting point ranging from 231 DEG C to 232 DEG C used for the control of crop diseases caused by fungi. The Oroxylin A is the secondary metabolite of plants widely existing in the oroxylum indicum of bignoniaceae, the scutellaria of labiatae and other species. The application of biological pesticide using Oroxylin A as the active ingredient in crop disease control has the advantages that, 1 the reagent using Oroxylin A as the active ingredient is an anti fungal infection reagent characterized in low toxicity, low residue, compatibility with environment and othercharacteristics, belonging to new biological pesticide and having obvious control effect to rice blast and vegetable and fruit gray mold; 2 the reagent has no or low direct bactericidal effect or bacteriostatic action, but the objective of disease control can be achieved by inducing the disease resistance responses of plants; 3 raw material source is relatively wide, and the promotion and the application of the invention are facilitated.
Description
Technical field
The present invention relates to qroxylin A (Oroxylin A, oroxylin) is the application of biopesticide in the control corps diseases of active ingredient, belongs to biological technical field.
Background technology
Along with the extensive concern of the whole world to resource and environment, " organic farming ", " pollution-free food " become the direction of agricultural development, and seek that exploitation is efficient, new direction that low toxicity, low-residual and the biorational agricultural chemicals of environment harmony progressively become pesticide research.Paddy rice and vegetables all belong to the crops of establishing in large scale.Rice blast is claimed rice blast again, is global important disease, and all there is generation in China north and south rice district.The popular area of disease, general underproduction 10%-20%, heavy reached at 40%-50%, the field piece of especially severe even No kernels or seeds are gathered, as in a year of scarcity.Gray mold is an important disease on the fruit and vegetables such as plastic tent cucumber, tomato, capsicum, strawberry, and this disease is popular rapidly, and regular meeting causes the massive losses of fruit and vegetable plantation.At present, these two kinds of disease controls on agricultural mainly are to adopt chemical pesticide control, but chemical pesticide exists pollution big, and poor with Environmental compatibility, pathogen is easy to generate problems such as pesticide resistance.Along with people's is to the pay attention to day by day of food-safety problem, and the public nuisance-free agricultural chemicals of development of new becomes exigence.
Qroxylin A is the Secondary Metabolism of Plant product that is present in the plant, and it is present in Oroxylum indicum and Huang Cenzhong usually.Qroxylin A has stronger antifungal activity, and the Oroxylum indicum and the Huang Cen that contain qroxylin A are conventional Chinese medicines, and Oroxylum indicum mainly contains moistening lung, relax liver and stomach effect; The root of large-flowered skullcap mainly contains anti-inflammatory, analgesic, step-down, diuresis.The function of qroxylin A in plant also do not understood, and do not appear in the newspapers yet.
Summary of the invention
Technical problem to be solved by this invention is to utilize qroxylin A (Oroxylin A; Oroxylin) antifungal activity; With its biopesticide as the control corps diseases; Stop the infringement of fungi effectively,, make it become the biopesticide of a kind of efficient, low toxicity, low-residual for qroxylin A provides a kind of new purposes to crops.
The present invention for the technical scheme that problem adopted of the above-mentioned proposition of solution is: described qroxylin A is 5, and 7-dihydroxy-6-methoxy flavone, yellow acicular crystal, fusing point are 231～232 ℃, is used to prevent and treat crops by fungus-caused disease.
Qroxylin A of the present invention has significant antibacterial activity, and they are secondary metabolites of plant, extensively is present in the species such as Bignoniaceae Oroxylum indicum, the Labiatae root of large-flowered skullcap, can be from plant, or be the synthetic material.
Through this technical field known organic solvent extraction, organic solvent extractionprocess, column chromatography obtain qroxylin A from the root of large-flowered skullcap (Scutellaria baicalensis) or Oroxylum indicum (Oroxylum indicum).
Show that through test qroxylin A of the present invention does not have or only have very low direct sterilization or bacteriostasis to germ, but can reach the purpose of preventing and treating disease through the disease resistance response of inducing plant.
Live test through to crops shows that particularly rice blast, vegetable and fruit gray mold have good control efficiency to qroxylin A of the present invention to corps diseases.
When manufacturing is the biopesticide of active ingredient with qroxylin A of the present invention; Add that agricultural goes up acceptable carrier, surfactant adjuvant, can be made into various carriers and various formulations such as the general solid phase of agricultural chemicals known in the technical field, liquid phase and emulsification, dispersant.Like wetting powder, missible oil, microemulsion, aqua, suspending agent, water dispersible granules etc.The proportioning of qroxylin A effective ingredient is 2-50% (W/W) by weight in each preparation, also can suitably increase and decrease according to different application targets.
Described biopesticide is used and is suitable for before disease takes place, using 1 time, re-uses 1-2 time at a distance from 7-10 days according to disease incidence and development situation then.Application dosage is the application dosage of conventional pesticide.
Beneficial effect of the present invention is: the medicament that 1, with the qroxylin A is effective ingredient is the secondary metabolite that plant produces in growth and development process; It is a kind of novel antimycotic medicament that infects; Have characteristics such as low toxicity, low-residual and environmentally compatible; Belong to novel biopesticide, rice blast and eliminating vegetable botrytis are had tangible control efficiency; 2, this medicament does not have or only has very low direct sterilization or bacteriostasis to germ, but can reach the purpose of preventing and treating disease through the disease resistance response of inducing plant; 3, raw material sources are extensive relatively, help promoting the use of.
Embodiment
In order to understand the present invention better, below in conjunction with embodiments of the invention, essentiality content of the present invention is described further, but content of the present invention is not limited thereto.
Embodiment 1: compound of the present invention is to the inhibitory action research of Pyricularia oryzae Cav bacterium
1, bacterial classification: Pyricularia griseawo2-1 (affine) with Lijiang xintuanheigu, sexual generation Magnaporthe grisea (Hebert) Barrnov
2, rice varieties: the rice varieties Lijiang xintuanheigu does not contain any anti-pest gene
It is active to the inhibition of rice blast fungus to adopt the pastille medium therapy to measure qroxylin A.Rice blast fungus is for use after PDA (potato glucose agar medium) goes up activation 72h earlier.Qroxylin A with dissolve with ethanol after; Be diluted to variable concentrations gradient 4000ug/g-40ug/g (i.e. each 10 times of concentration handling) respectively; With PDA dissolve to be cooled during to 50 ℃ with soup and PDA medium mix at 1: 9 (v/v) back the pastille medium of 400ug/ml～4ug/ml; 4 flat boards (diameter 9cm) are processed in every processing, insert 6mm bacterium cake in dull and stereotyped central authorities after cooling, put 25 ± 1 ℃ and cultivate down; Respectively handle colony diameter with slide measure right-angled intersection method mensuration when treating blank bacterium cake length, calculate and respectively handle bacteriostasis rate to plate edge.Bacteriostasis rate %=(blank colony diameter-processing colony diameter)/(blank colony diameter-bacterium cake diameter) * 100%, the result proves that qroxylin A does not have direct fungistatic effect to rice blast on medium.
Adopt the leaf section method that exsomatizes to measure inhibition effect to rice blast.The presoaking and germinating basin is broadcast the paddy rice Lijiang xintuanheigu; When paddy rice 5 leaves 1 heart; The 5th leaf haircut is truncated; Get the 5cm section of coming into leaves and put and respectively handle in the culture dish, every ware 5-7 section, (qroxylin A is that 400ug/ml～4ug/ml) carries out 3 sprayings that repeat the 5ml amount in spray tower to adopt 2 times of concentration dilution methods then.The leaf section that will exsomatize again after 24 hours inoculation 1 * 10
6The spore liquid of individual/ml concentration, 3 of every processing repeat quantitatively spraying 5ml bacterium liquid, under 26 ℃ of dark conditions, preserve moisture and cultivate 36h, and illumination 36h investigates incidence then again, calculates control efficiency.Control efficiency %=(contrast scab number-processing scab number)/contrast scab number * 100.The result proves that qroxylin A has good fungistatic effect to rice blast.Pointed out qroxylin A that rice blast is had the inducing anti-disease fungistatic effect.
50% (W/W) aqua of present embodiment 2 is used in the field, when paddy rice sporadicly falls ill (Sheng of tillering phase) begin to use, 7 days 1 time at interval, totally 3 times, in rice blast typing " Invest, Then Investigate ".The result proves that qroxylin A can effectively suppress the generation of field rice rice blast.
Embodiment 2:50% aqua.
By weight with 50 parts of (W/W) qroxylin As with 10 parts of dissolve with ethanol after, add 5 parts of 656H compound auxiliaries and mix, add 35 parts of water, promptly get 50% aqua.
Embodiment 3: qroxylin A is to vegetable botrytis cinerea (Botrytis cinerea) indoor biometrics and field controling test.
According to embodiment 1 same method survey is cultivated and given birth to vegetable botrytis cinerea (Botrytis cinerea), starts in 3 leaf periods of cucumber and begins after being ill to use in the field, and 7 days 1 time at interval, totally 3 times, in gray mold of cucumber typing " Invest, Then Investigate ".The result proves that qroxylin A does not have direct fungistatic effect to rice blast equally on medium.Adopt the leaf section method that exsomatizes to measure the vegetable botrytis cinerea disease is had the obvious suppression effect equally.Field controling test result proof can effectively suppress the generation of land for growing field crops gray mold of cucumber.
Claims (6)
1. qroxylin A is as the application of biopesticide in the control corps diseases; It is characterized in that described qroxylin A is 5,7-dihydroxy-6-methoxy flavone, yellow acicular crystal; Fusing point is 231～232 ℃, is used to prevent and treat crops by fungus-caused disease.
2. qroxylin A according to claim 1 is characterized in that as the application of biopesticide in the control corps diseases described qroxylin A can reach the purpose of preventing and treating disease through the disease resistance response of inducing plant.
3. qroxylin A according to claim 1 and 2 in the application of control in the corps diseases, is characterized in that the fungal disease that described qroxylin A is used to prevent and treat crops is rice blast and vegetable and fruit gray mold as biopesticide.
4. qroxylin A according to claim 1 and 2 is as the application of biopesticide in the control corps diseases; When it is characterized in that qroxylin A is the biopesticide of active ingredient; Add that agricultural goes up acceptable carrier, surfactant adjuvant; Process wetting powder, missible oil, microemulsion, aqua, suspending agent, water dispersible granules, the proportioning of qroxylin A effective ingredient is 2-50% by weight in each preparation.
5. qroxylin A according to claim 4 is as the application of biopesticide in the control corps diseases; It is characterized in that described biopesticide is used is suitable for before disease takes place, using 1 time, re-uses 1-2 time at a distance from 7-10 days according to disease incidence and development situation then.
6. qroxylin A according to claim 3 is characterized in that obtaining qroxylin A through organic solvent extraction, organic solvent extractionprocess, column chromatography from the root of large-flowered skullcap or Oroxylum indicum as the application of biopesticide in the control corps diseases.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100472153A CN101263813B (en) | 2008-04-02 | 2008-04-02 | Application of oroxylin A as biological pesticide in preventing and controlling corps diseases |
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| CN2008100472153A CN101263813B (en) | 2008-04-02 | 2008-04-02 | Application of oroxylin A as biological pesticide in preventing and controlling corps diseases |
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| CN101263813A CN101263813A (en) | 2008-09-17 |
| CN101263813B true CN101263813B (en) | 2012-01-11 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111320601B (en) * | 2018-12-13 | 2022-12-13 | 中国药科大学 | Oroxylin crystal form and preparation method thereof |
| CN111920800B (en) * | 2020-08-26 | 2023-06-13 | 中国人民解放军海军军医大学第一附属医院 | Application of oroxylin A in treating osteoporosis and promoting fracture healing |
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2008
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Non-Patent Citations (11)
| Title |
|---|
| Buschi, Carlos A. |
| Funayama, Shinji |
| Funayama, Shinji;Komiyama, Kanki;Miyaichi, Yukinori;Tomimori, Tsuyoshi;Nozoe, Shigeo.Cytocidal and antimicrobial activities of flavonoids.Natural Medicines49 3.1995,49(3),322-8. * |
| Komiyama, Kanki |
| Miyaichi, Yukinori |
| Nozoe, Shigeo.Cytocidal and antimicrobial activities of flavonoids.Natural Medicines49 3.1995,49(3),322-8. |
| Pomilio, Alicia B. |
| Pomilio, Alicia B.;Buschi, Carlos A.;Tomes, Claudia N.;Viale,AlbertoA.Antimicrobial constituents of Gomphrena martiana andGomphrena boliviana.Journal of Ethnopharmacology36 2.1992,36(2),155-61. * |
| Tomes, Claudia N. |
| Tomimori, Tsuyoshi |
| Viale,AlbertoA.Antimicrobial constituents of Gomphrena martiana andGomphrena boliviana.Journal of Ethnopharmacology36 2.1992,36(2),155-61. |
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