CN105432381A - Prevention and control method of pepper phytophthora blight - Google Patents

Abstract

The invention discloses a prevention and control method of pepper phytophthora blight. The method specifically includes the steps of clearing away residual preceding crops in a large field, applying fertilizer and organic fertilizer according to a conventional method, mixing trichoderma viride with charcoal, evenly spreading and applying the mixture according to the application amount of 300-400 kg/mu, conducting rotary tillage on soil by 20-25 cm, conducting watering and irrigating till the soil water content is 18-20%, keeping the state for 10-30 days, and planting pepper. The conventional prevention and control method that in the prior art, fungicide is used when and after pepper is planted is broken through, charcoal with trichoderma viride is applied in soil 10-30 days ahead, the stable microflora with functional microorganisms as the dominant population is formed for soil within a sufficient period of time, the environment not beneficial to survival of pathogenic bacteria is created so that pepper phytophthora blight can be prevented and controlled, the method is simple in application process, easy to operate and suitable for large-area application and popularization, and the prevention effect reaches up to 90-100%.

Description

A kind of preventing control method of capsicum epidemic disease

Technical field

The present invention relates to crop disease control field, particularly a kind of preventing control method of capsicum epidemic disease.

Background technology

The soil-borne disease that capsicum epidemic disease is caused by P. capsici, worldwide generally occur.This disease is be found in pepper producing area in 1918 seriously to occur the earliest, and the capsicum epidemic disease of this diseased region occurs year after year subsequently, and constantly spreads to surrounding area.At present, capsicum epidemic disease in America, Asia, multiple countries and regions such as Europe generally occur, and have become one of great disease of restriction capsicum industry sustainable development.In nineteen fifty, China finds in Jiangsu that this is sick first, subsequently, Gansu Province 1987, Shaanxi Province there occurs capsicum epidemic disease in 1989 and is very popular, present capsicum epidemic disease generally occurs in China pepper producing area, and have the trend increasing the weight of year by year and spread, become the bottleneck of serious restriction Hot Pepper Industry Development.

The generation climate of capsicum epidemic disease, soil types, capsicum variety, the various factors such as irrigation method and field management level, the death rate caused because of this kind of disease reaches 15.1%, occur serious can underproduction 40-70%, even have no harvest.At present, the method both at home and abroad for preventing and treating capsicum epidemic disease mainly contains Agro-chemicals control, biological control, breeding resistant variety and agricultural measures etc.Although all there is reporting for work of long investigation and application to every Prevention Technique, and still constantly making progress and development, still lacking in production aspect large-scale promotion application aspect that preventive effect is stablized, the prevention and control measure of economically feasible, operability.

Charcoal (biochar) is by living beings solid products that Pintsch process (being usually less than 700 DEG C) produces under anaerobic or anoxia condition such as the residual body of biology, organic wastes, in charcoal processing process, the micropore structure of primordial matter is retained in charcoal in good condition, and this porous makes the charcoal prepared have larger surface area; In addition, the typical structures such as carboxyl, phenolic hydroxyl group, hydroxyl, aliphatic double bonds and aromatization, charcoal is possessed extremely strong adsorption capacity, oxidation resistance and antibiont capacity of decomposition etc., thus make charcoal be widely used in the fields such as agricultural, industry, environment, the energy.The effect of charcoal in soil mainly contains: improve soil aggregate, reduce soil acidity, improve soil water-retaining, nutrient preserving capability and plant recovery of nutrient, promote mycorrhizal fungi, rhizobium and other beneficial microorganism growth and breedings, Promoting plant growth and raising output, prevention and control plant disease, the poisonous and harmful substances such as the heavy metal in absorption soil and organic pollutant.Research shows to be manured into soil with charcoal prepared by domestic waste to reduce the microbial bacterial wilt of tomato of bacterial wilt; Ogawa research finds charcoal and charcoal and compost mixture all energy anti-bacteria and fungus-caused soil-borne diseases, and the charcoal having report openly to use eucalyptus wood and greenhouse organic waste to prepare in addition all can promote cucumber growth and suppress the microbial cucumber damping off of miliary damping-off.

Charcoal has larger correlation to the prevention and control effect of soil-borne disease and its to the improvement of edaphon proterties, is mainly manifested in: the micro organism quantity and the microbial activity that 1) improve soil or plant rhizosphere; 2) promote that mycorrhizal fungi is at the infection and reproduction of plant root; 3) beneficial microorganism growth is promoted; 4) biological community structure is improved.First charcoal shows the impact of edaphon increases soil microbe quantity and microbial activity, the porous of charcoal is bacterium, the survival and reproduction of actinomycetes and fungi provides habitat, and be beneficial to the invasion and attack that microorganism hides the predatory animal of soil, there is certain protective role to edaphon; Steinbeiss reports that charcoal promotes the growth of soil Gram-negative bacteria; Elad test data display charcoal can increase the quantity of cultivable bacteria in soil, actinomycetes, saccharomycete and filamentous fungi (comprising wood mould) etc.But also having correlative study to show, charcoal is unstable to the control effect of soil-borne disease after being administered to soil, does not sometimes even have control effect.This main cause is that the kind randomlikeness of the edaphon excited by time of application and the charcoal of charcoal is comparatively large, predictable less, can not form the soil environment of stabilization checking disease.

Trichoderma viride is due to its fast growth, biomass greatly and stronger ecologic competition ability, to the growth inhibition effect of many plant pathogenic fungis and the facilitation to plant growth, academy of agricultural sciences of Jiangsu Province screens the Trichoderma viride Trichoderma viride that a strain preserving number is CGMCCNo.9293 from soil, and be 201510361863.6 at application number, denomination of invention be " method of efficient prevention and control watermelon blight and special microorganism bacterial strain thereof " and patent document in open, this bacterium has strong growth inhibition effect to withered germ of water-melon, but this bacterium is mixed with charcoal and uses, control capsicum epidemic disease, there is not yet relevant report.

Summary of the invention

For the problems referred to above, the invention provides the method for a kind of simple to operate, preventive effect up to the prevention and control capsicum epidemic disease of 90-100%, the present invention is achieved in that

Utilize a method for charcoal prevention and control capsicum epidemic disease, concrete steps are as follows:

First the residual body of large Tanaka's preceding crop is removed, according to a conventional method to fertilize and fertilizer, then trichoderma viride is mixed with charcoal, mixture evenly spreads fertilizer over the fields with the amount of application of 300-400 kg/acre, again by soil rotary tillage 20-25cm, irrigation to the soil water content that waters is 18-20%, after placing 10-30d, then capsicum of planting; Described trichoderma viride refers to that preserving number is the Trichoderma viride TV41 bacterium of CGMCCNo.9293.

Further, in the present invention, described mixing with charcoal by trichoderma viride refers to: cross 2 mm sieve after being pulverized by charcoal, and then add the Trichoderma viride TV41 microbial inoculum mixing that preserving number is CGMCCNo.9293, the bacteria containing amount of the mould TV41 bacterium of mixture Green wood is 2X10 ⁷cfu/g; Described charcoal obtains after being fired through 500 DEG C by corn, wheat or rice straw.

Further, in the present invention, described Trichoderma viride TV41 microbial inoculum obtains like this:

(1) be that the Trichoderma viride TV41 bacterium of CGMCCNo.9293 is inoculated in test tube slant medium PDA by preserving number, cultivate 3 days for 28 ± 2 DEG C; Be transferred to by trichoderma viride strain in PDB liquid nutrient medium, liquid amount is 80ml/250ml again, and then at 28 ± 2 DEG C of temperature, shaking table cultivates 3-4 days, the rotating speed 160rpm of shaking table;

(2) cultivate terminate after with 5% inoculum concentration V/W access in solid culture medium, cultivate 6-8 days in 28 ± 2 DEG C, period turned once every 2-3 days, until produce spore;

(3) by trichoderma viride together with solid culture medium natural air drying or 30 DEG C of oven dry, pulverized 10-20 mesh sieve, namely obtain Trichoderma viride solid fungicide, its bacteria containing amount is 10 ^10-11cfu/g;

Described test tube slant medium PDA compound method is: take potato 100 grams, peeling, is cut into block and boils half an hour, then use two-layer filtered through gauze, add glucose 10 grams again, 10 grams, agar, supplies water to 500 milliliter after dissolving, and is dispensed in the test tube of 25 milliliters, each test tube 5 milliliters of medium, 121 DEG C, be put into inclined-plane for subsequent use after sterilizing 15min, bevel altitude is no more than 1/3rd of test tube length;

Described liquid nutrient medium PDB compound method is: take potato 100 grams, peeling, be cut into block and boil half an hour, then use two-layer filtered through gauze, then add human glocose 10 grams, after dissolving, supply water to 500 milliliter, be dispensed in triangular flask, bottled 80 milliliters of each 250 milliliters of triangles, cool for subsequent use by 121 DEG C after sterilizing 15min;

The compound method of described solid culture medium is: by wheat bran and the Homogeneous phase mixing of straw according to mass ratio 7:3 being not more than 5mm length, then adds deionized water and make the moisture in mixture be 65%, and then 121 DEG C of sterilizing 40min, cool for subsequent use.

The present invention breaks the conventional control method re-using microbial inoculum in prior art when capsicum field planting or after field planting, by the charcoal used in soil for 10-30 days containing Trichoderma viride in advance, soil being had ample time formed take functional microorganism as the stable microflora of dominant population, build and be unfavorable for the environment that pathogen survives, then prevention and control capsicum epidemic disease, its beneficial effect is embodied in:

(1) physics of soil, chemical property excite the feature of soil multiple-microorganism activity can be improved based on charcoal, the present invention uses straw biological charcoal improvement capsicum epidemic disease for 10-30 days before capsicum field planting and serious soil occurs, build and be unfavorable for the environment that growth of pathogenic bacteria is bred, reduce pathogen quantity, reduce disease occurrence probability, improve the control effect to capsicum epidemic disease, for the high value added utilization of straw biological charcoal opens up new way;

(2) the Trichoderma viride microbial inoculum containing wide spectrum disease-proof functions in the charcoal used, this Trichoderma viride reproduction speed in soil is fast, and have strong growth inhibition effect to Phytophthora capsici, can reach stable, continue to suppress pathogen quantity to increase, strengthen the object of disease control effect.Whole application is simple, is easy to operation, is suitable for spread and uses; The Trichoderma viride used has stronger competition and fertility at soil and capsicum rhizosphere, optimizes the microflora composition of capsicum, plays promoting crop growth, protection effect, for the efficiency utilization of the control of protecting field soil-borne disease and microbial resources opens up new approach.

(3) after within 10-30 days, applying the charcoal containing Trichoderma viride before capsicum is transplanted, the soil organic matter, rapid available phosphorus and quick-acting potassium content increase, soil bacteria and fungi total number significantly increase, in soil, educable antagonistic microbe quantity obviously increases, the multiple the enzyme activity of soil improves, Phytophthora capsici quantity reduces, the incidence of disease of capsicum epidemic disease is than the remarkable reduction of contrast, preventive effect can reach 90-100%, pepper plant biomass improves 10-20% than not using charcoal, significant to the sustainable development of facility operation character improvement and sustainable use and industrialized agriculture.

Accompanying drawing explanation

Fig. 1 is that embodiment Trichoderma viride is to P. capsici bacteriostatic test result schematic diagram.

Fig. 2 is the control effect schematic diagram of embodiment different disposal to capsicum epidemic disease.

Embodiment

The culture medium prescription related in embodiment:

Test tube slant medium PDA compound method is: take potato 100 grams, peeling, is cut into block and boils half an hour, then use two-layer filtered through gauze, add glucose 10 grams again, 10 grams, agar, supplies water to 500 milliliter after dissolving, and is dispensed in the test tube of 25 milliliters, each test tube 5 milliliters of medium, 121 DEG C, be put into inclined-plane for subsequent use after sterilizing 15min, bevel altitude is no more than 1/3rd of test tube length.

The compound method of PDA flat board is: take potato 100 grams, and peeling, is cut into block and boils half an hour, then two-layer filtered through gauze is used, add glucose 10 grams again, 10 grams, agar, supplies water to 500 milliliter after dissolving, average mark installs in the triangular flask of 5 250 milliliters, 121 DEG C, after sterilizing 15min, be cooled to 55-60 DEG C, 100 milliliters of medium evenly poured in the sterile petri dish of 5 diameters 8.5 centimetres in superclean bench, about 20 milliliters of medium poured into by each culture dish, for subsequent use after cooling.

Liquid nutrient medium PDB compound method is: take potato 100 grams, peeling, be cut into block and boil half an hour, then use two-layer filtered through gauze, then add human glocose 10 grams, after dissolving, supply water to 500 milliliter, be dispensed in triangular flask, bottled 80 milliliters of each 250 milliliters of triangles, cool for subsequent use by 121 DEG C after sterilizing 15min.

The compound method of solid culture medium is: by wheat bran and the Homogeneous phase mixing of straw according to mass ratio 7:3 being not more than 5mm length, then adds deionized water and make the moisture in mixture be 65%, and then 121 DEG C of sterilizing 40min, cool for subsequent use.

The microbial inoculum source related in embodiment:

The trichoderma viride (Trichodermaharzianum) used in embodiment is the Trichoderma viride TV41 bacterium of CGMCCNo.9293 for preserving number, is provided by Jiangsu Province Agriculture Science Institute.

Phytophthora capsici is provided by Chinese Academy of Sciences's Culture Collection.

Embodiment 1 prepares Trichoderma viride microbial inoculum

A) activation of bacterial classification

Adopt the activation method of test tube slant preservation of bacteria strain: the inoculated by hypha block of trichoderma viride picking one piece of diameter about 5mm of test tube slant preservation activated in test tube slant medium PDA, adopt the activation method of ampoul tube lyophil preservation bacterial classification: in superclean bench, clean ampoule with 70% cotton ball soaked in alcohol, then in ampoule, one ditch is frustrated with emery wheel, ampoule is wrapped with sterile gauze pad or sterile towel, then break ampoul tube into two with one's hands with hand and add 0.5-1.0ml liquid nutrient medium PDB wherein, slowly rotate ampoule, make freeze-drying lactobacillus rehydration, and then this is transferred in test tube slant medium PDA activates, cultivate after 3-5 days for subsequent use in the environment of 28 ± 2 DEG C.

B) liquid culture

The bacterium block of described Trichoderma viride picking three pieces of diameter about 8mm that test tube is activated be seeded in respectively three volumes be 250ml be equipped with in 80ml liquid nutrient medium PDB triangular flask, be placed in shaking table, the rotating speed 160r/min of shaking table, cultivates after 3-4 days for subsequent use in the environment of 28 ± 2 DEG C.

C) solid culture

By in 250ml Trichoderma viride culture good for liquid culture access 5kg solid culture medium, solid culture is laid in (tray is used after formalin solution soaked overnight is dried) in tray, thickness 5-8cm, the double-deck gauze through autoclaving process in upper cover, continue to cultivate 6-8 days in 28 ± 2 DEG C of environment, turned once every 2-3 days, suitably add sterile water simultaneously, be cultured to product spore; Last natural air drying or 30 DEG C of oven dry (moisture is less than 10%) are Trichoderma viride microbial inoculum, and microbial inoculum bacteria containing amount is 10 ^10-11cfu/g.

The all inoculation operations of the present embodiment must aseptically, and namely on the alcolhol burner side of superclean bench or through the ultraviolet disinfection process room of 1-2 hour, the room of solid culture also needs in advance with ultraviolet disinfection process 1-2 hour

The preparation of the charcoal of embodiment 2 containing Trichoderma viride TV41 bacterium (calling in the following text containing the mould biology of wood)

By broken for cornstalk biological powdered carbon rear mistake 2 mm sieve, then add the solid fungicide of Trichoderma viride TV41, mixing, makes to add wooden mould quantity in artifact charcoal and is not less than 2X10 ⁷cfu/ gram, is placed on shady and cool dry place for subsequent use.

In concrete production, also can use the charcoal that wheat or rice straw are fired.

Embodiment 3 trichoderma viride is to the growth inhibition test of Phytophthora capsici

Test method: trichoderma viride and Phytophthora capsici are individually inoculated on PDA flat board, 30 DEG C of constant temperature culture are after 3 days, obtain the fresh mycelia block (diameter 5 millimeters) of each bacterial strain, again Trichoderma viride and Phytophthora capsici mycelia block are inoculated into (each plating 1 Trichoderma viride mycelia block and 1 Phytophthora capsici mycelia block) in same PDA flat board, at a distance of 5 centimetres between two mycelia blocks, be placed in 30 DEG C of insulating boxs and cultivate observation.

Result of the test as shown in Figure 1, wherein, Fig. 1 (A) is cultivation observed result after 24 hours, Fig. 1 (B) is cultivation observed result after 48 hours, in Fig. 1 culture dish, top is Phytophthora capsici mycelia, and bottom is Trichoderma viride mycelia, visible, this trichoderma viride has obvious growth inhibition effect to P. capsici.Find after further research, Trichoderma viride is mainly suppressed by hyperparasitism or kills pathogen, namely reaches by the multiple enzyme of the multiple composite antibacterial material of secretion or pathogen cell wall of degrading the effect suppressing target pathogens growth.

Embodiment 4 charcoal and Trichoderma viride TV41 compounding application effect test

Test the mode utilizing basin pot culture to train in money ring institute of academy of agricultural sciences of Jiangsu Province booth to carry out, natural lighting, temperature 25-35 DEG C.

Test method is: evenly added in soil by the Phytophthora Capsici Zoospores liquid prepared (preparation method is shown in that document Wang Guang flies (2015, microorganism journal)), make soil Phytophthora capsici quantity be 80 zoospores/gram dry ground.4 process are established in test, are followed successively by:

(1) contrast, soil does not add the mould and charcoal of wood;

(2) wooden mould TV41, adds the Trichoderma viride microbial inoculum that embodiment 1 obtains, makes wooden mould quantity in soil be 10 in soil ^{5- 6}cfu/ gram of soil;

(3) charcoal, charcoal amount of application is 1.0% of soil quality;

(4) wooden mould TV41+ charcoal, evenly adds the charcoal containing wooden mould TV41 with 1% of soil quality, makes quantity in soil be 10 ^5-6cfu/ gram of soil

Each process soil is dispensed in 10 basin round mouth basin alms bowls, the fresh soil of every basin 500g, and after packing, namely six leaf phase Hot Pepper Seedlings planted by every basin.Each process three repetition, namely often processes totally 30 basins.Experimental period 30 days, at the 15th day with within 30 days, add up the incidence of disease of each process respectively.

Under table 1 different disposal, the incidence of disease (%) of capsicum epidemic disease

	Contrast	The mould TV41 of wood	Charcoal	The mould TV41+ charcoal of wood
					15 days	40	10	20	10
30 days	90	50	40	20

As can be seen from Table 1, be used alone wooden mould TV41 or charcoal all has certain control effect to capsicum epidemic disease, but preventive effect is not high, and by both composite rear uses, then significantly improve disease control effect, from latter 15 days to 30 days of transplanting, the incidence of disease of composite use is only 10%, and be used alone wood the incidence of disease that is mould or charcoal be respectively 40% and 20%, visible, use containing wood mould charcoal can significantly improve preventive effect.

Embodiment 5 contains the wood mould charcoal different administration time to the impact of the capsicum epidemic disease incidence of disease

Test the mode utilizing basin pot culture to train in money ring institute of academy of agricultural sciences of Jiangsu Province booth to carry out, natural lighting, temperature 25-35 DEG C.

Phytophthora Capsici Zoospores liquid (preparing same embodiment 4) is evenly added in soil, makes soil Phytophthora capsici quantity be 80 zoospores/gram dry ground; Trichoderma viride microbial inoculum embodiment 1 obtained mixes with charcoal, and obtain containing the mould charcoal of wood, wherein Trichoderma viride bacteria containing amount is 2X10 ⁷cfu/g.

5 process are established in test, are followed successively by:

(1) CK0, contrast, soil is left intact;

(2) T0 is 1.0% of soil quality containing the mould charcoal amount of application of wood; Capsicum is transplanted immediately after using;

(3) T10 is 1.0% of soil quality containing the mould charcoal amount of application of wood; Capsicum is transplanted and uses for first 10 days;

(4) T20 is 1.0% of soil quality containing the mould charcoal amount of application of wood; Capsicum is transplanted and uses for first 20 days;

(5) T30 is 1.0% of soil quality containing the mould charcoal amount of application of wood; Capsicum is transplanted and uses for first 30 days.

Add soil by above-mentioned mass ratio containing the mould charcoal of wood and mix, during placing 10-30 days, making soil moisture content remain on 18-20%, process terminates, each process soil is dispensed in 15 basin round mouth basin alms bowls, the fresh soil of every basin 500g, and six leaf phase Hot Pepper Seedlings planted by every basin.Each process three repetition, namely often processes totally 45 basins.15 days after transplanting, 30 days and 45 days periodic statistical incidences of disease, result of the test is as shown in table 2 and Fig. 2:

The incidence of disease (%) of capsicum epidemic disease under table 2 different disposal

	CK0	T0	T10	T20	T30
						15 days	40	15	5	5	5
30 days	70	40	20	10	10
						45 days	100	50	20	15	15

Fig. 2 is that capsicum is transplanted latter 45 days, different disposal is to the control effect schematic diagram of capsicum epidemic disease, as can be seen from table 2 and Fig. 2, compared with the control, use the process containing the mould charcoal of wood, the capsicum epidemic disease incidence of disease significantly reduces, with namely transplant compared with capsicum after using, and within 10-30 days, use containing the mould charcoal of wood in advance, control effect is significantly improved, and comparatively steady between the control effect of three kinds of service times, so consider the practicality in agricultural production, suggestion uses more than 10 days in advance containing the mould charcoal of wood.

Use under embodiment 6 field condition containing wooden mould charcoal the control effect of capsicum epidemic disease

Test is carried out at Qingpu District, Huai'an capsicum continuous cropping booth, the soil treatment time is August, booth area is 1 mu, this booth plants capsicum for many years continuously, capsicum epidemic disease occurs serious, residual for preceding vegetable body is removed clean, by trench digging in the middle of booth, be divided into two, be set to Routine control and process two groups, whole booth (i.e. control group and experimental group) first normal homogeneous applies 1 ton of general goods fertilizer and 40 kilograms of composite fertilizers (15-15-15), then 150-200 kilogram is evenly spread fertilizer over the fields again containing wooden mould charcoal in processed group, conventional group does not process, again whole booth is turned over 20-25 centimetre dark, rewater to water content be 18-20%, maintain after moisture places 20 days and transplant capsicum, respectively after transplanting 30, 60, 90 days investigation Field diseases, result is as shown in table 3:

The incidence of disease (%) of capsicum epidemic disease under the different disposal of table 3 land for growing field crops

	30 days	60 days	90 days
				Routine control	8	15	37
The mould TV41+ charcoal of wood	0	8	12

Table 3 is different time after capsicum transplanting, different disposal is to the control effect of capsicum epidemic disease, as can be seen from Table 3, compared with Routine control, use the process of wooden mould TV41+ charcoal, the capsicum epidemic disease incidence of disease significantly reduces, and transplants latter 30 days, contrasts the incidence of disease of 8%, and to process the incidence of disease be 0, transplant latter 60 days and 90 days, the Routine control incidence of disease is increased to 37% by 15%, and the incidence of disease of process only brings up to 12% from 8%, not only reduce the incidence of disease, and reducing disease development speed, during by 90 days, control effect reaches 67%.

These are only the preferred embodiments of the present invention, not limitation of the present invention.In concrete implementation process, those skilled in the art, without departing from the inventive concept of the premise; some improvement can also be made; as replaced maize straw to fire charcoal with wheat or rice straw, to realize the object of the present invention, these improvement all belong to protection scope of the present invention.

Claims (3)

1. a preventing control method for capsicum epidemic disease, is characterized in that, concrete steps are as follows:

First the residual body of large Tanaka's preceding crop is removed, according to a conventional method to fertilize and fertilizer, then trichoderma viride is mixed with charcoal, mixture evenly spreads fertilizer over the fields with the amount of application of 300-400 kg/acre, again by soil rotary tillage 20-25cm, irrigation to the soil water content that waters is 18-20%, after keeping 10-30d, then capsicum of planting;

Described trichoderma viride refers to that preserving number is the Trichoderma viride TV41 bacterium of CGMCCNo.9293.

2. the preventing control method of a kind of capsicum epidemic disease according to claim 1, it is characterized in that, described mixing with charcoal by trichoderma viride refers to: cross 2 mm sieve after being pulverized by charcoal, adds the mixing of Trichoderma viride TV41 microbial inoculum, and the bacteria containing amount of the mould TV41 bacterium of mixture Green wood is 2X10 ⁷cfu/ gram.

3. the preventing control method of a kind of capsicum epidemic disease according to claim 2, is characterized in that, described Trichoderma viride TV41 microbial inoculum obtains like this:

(1) will be inoculated into as the trichoderma viride strain weighed as described in 1 in test tube slant medium PDA, cultivate 3 days for 28 ± 2 DEG C; Be transferred to by trichoderma viride strain in PDB liquid nutrient medium, liquid amount is 80ml/250ml again, and then at 28 ± 2 DEG C of temperature, shaking table cultivates 3-4 days, the rotating speed 160rpm of shaking table;

(2) after cultivation terminates, access in solid culture medium by the volume mass of 5% than by trichoderma viride, cultivate 6-8 days in 28 ± 2 DEG C;

(3) by trichoderma viride together with solid culture medium natural air drying or 30 DEG C of oven dry, pulverized 10-20 mesh sieve, namely obtain Trichoderma viride solid fungicide, its bacteria containing amount is 10 ^10-11cfu/g;

The compound method of described solid culture medium is: by wheat bran with the straw no longer than 5mm according to the Homogeneous phase mixing of mass ratio 7:3, then add deionized water and make the moisture in mixture be 65%, then 121 DEG C of sterilizing 40min.