CN103467185A - Biological compound organic bacterial fertilizer - Google Patents

Biological compound organic bacterial fertilizer Download PDF

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CN103467185A
CN103467185A CN2013103718851A CN201310371885A CN103467185A CN 103467185 A CN103467185 A CN 103467185A CN 2013103718851 A CN2013103718851 A CN 2013103718851A CN 201310371885 A CN201310371885 A CN 201310371885A CN 103467185 A CN103467185 A CN 103467185A
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fertilizer
manure
described
compound
microbial inoculum
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CN2013103718851A
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CN103467185B (en
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赵辉
刘红彦
鲁传涛
刘玉霞
杨修身
倪云霞
王飞
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河南省农业科学院植物保护研究所
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Abstract

The invention belongs to the technical field of fertilizer preparation, and particularly relates to a biological compound organic bacterial fertilizer which is composed of a compound fertilizer and a mixed microbial agent. The compound fertilizer comprises the following raw fertilizers in percentage by weight: 14.5% to 25.2% of humic acid, 33.5% to 45.1% of biochar, 22.5% to 35.5% of chemical fertilizer and 10.2% to 15.4% of bentonite; besides 10 mL to 20 mL mixed microbial agent is added into the compound fertilizer per kilogram. The biological compound organic bacterial fertilizer not only can be taken as an auxiliary fertilizer to be mixed with a chemical fertilizer for use, but also can be used alone, can effectively supply soil organic matters, activates P fertilizer and K fertilizer of soil, and improves the disease resistance of crops; particularly, the microbial agent is composited, so that the biological compound organic bacterial fertilizer has a better effect on the prevention and treatment of soil-borne disease and continuous cropping disease of the crops, and is efficient, nontoxic and environment-friendly.

Description

Biological compound organic fungi-manure

Technical field

The invention belongs to the Production of manure technical field, be specifically related to the compound organic fungi-manure of a kind of biology.

Background technology

At present, in agricultural production process, distinct issues mainly contain: use in a large number the soil compaction that chemical pesticide causes; Environmental pollution and the potential safety hazard that can not rationally utilize crop material, on-site incineration to bring; That uses chemical pesticide to cause is residual; The disease continuous cropping problem that continuous cropping brings; Fertilising separates with agricultural chemicals the problems such as labor waste that caused.These problems have caused serious hindrance to agriculture production and increasing crop yield.And the application in agricultural along with microbial technique, many function yeast also are widely used in agricultural chemicals and fertilizer field, for realizing that environmental protection, pollution-free and high-efficiency agriculture production provide effective means and solution route, therefore, the bio-feritlizer that many function yeast participate in has arisen at the historic moment, but current bio-feritlizer can only, as complementary fertilizer, reduce the consumption of chemical fertilizer and agricultural chemicals, and can not fundamentally solve the problems such as soil compaction and crop material utilization.

Summary of the invention

For the problems referred to above, the purpose of this invention is to provide a kind of auxiliary fertile use that mixes with chemical fertilizer that both can be used as, the compound organic fungi-manure of the biology can be used alone again, the present invention can effectively supplement the soil organism, activating soil P fertilizer and K fertilizer, improve crop disease-resistant ability, particularly complex microorganism, to preventing and treating soil-borne disease, the continuous cropping disease has effect preferably, is a kind of composite biological fertilizer of efficient, nontoxic, environmental protection.

The present invention is achieved through the following technical solutions:

The compound organic fungi-manure of a kind of biology, the compound organic fungi-manure of described biology is comprised of compound manure and mixing microorganisms microbial inoculum, contains the material fertilizer of following weight percent in described compound manure: humic acid 14.5%~25.2%, charcoal 33.5%~45.1%, chemical fertilizer 22.5%~35.5%, wilkinite 10.2%~15.4%; In addition, add 10mL~20mL mixing microorganisms microbial inoculum in every 1kg compound manure; Described chemical fertilizer composition is urea, secondary calcium phosphate and vitriolate of tartar, and its weight ratio is urea: secondary calcium phosphate: vitriolate of tartar=(40~45): (35~45): (10~20); Contain bacillus amyloliquefaciens, subtilis and three kinds of microbial inoculums of Trichoderma in described mixing microorganisms microbial inoculum, its volume ratio is bacillus amyloliquefaciens: subtilis: Trichoderma=(30~40): (35~45): (25~35).

According to the compound organic fungi-manure of above-mentioned biology, described bacillus amyloliquefaciens (1.2~2.0) * 10 8individual/mL, subtilis (2.0~2.5) * 10 8individual/mL, Trichoderma (2.5~3.0) * 10 8individual/mL.

According to the compound organic fungi-manure of the described biology of above any one, by following steps, be prepared from:

1) preparation of mixing microorganisms microbial inoculum: bacillus amyloliquefaciens, subtilis and three kinds of microbial inoculums of Trichoderma are mixed by described volume ratio;

2) preparation of chemical fertilizer: be equipped with urea, secondary calcium phosphate and vitriolate of tartar by described weight ratio, and mix;

3) preparation of charcoal: will drop into after crops stalk crushing in the charcoal high-temperature cracking furnace, under anoxia condition, process the generation charcoal for 300 ℃~500 ℃, and then pulverize, cross 200~300 mesh sieves and process, stand-by;

4) preparation of compound manure: the humic acids, charcoal, chemical fertilizer, the wilkinite that have been equipped with are mixed by weight, and stir;

5) spray in proportion described mixing microorganisms microbial inoculum in described compound manure, after evenly mixing, obtain biological compound organic fungi-manure.

positive beneficial effect of the present invention:

(1) mixing microorganisms microbial inoculum of the present invention has and suppresses and kill the pathogenic soil microorganism, improve crop resistance, improve the effect of damage of successive crop; The crop material charcoal generated under the high temperature anoxia condition has activating soil P element and K element and improves the effect of soil microorganisms environment, can effectively treat soil compaction; Humic acids has the effect of regulating soil pH value and improving soil organic matter content.

(2) the present invention's bacterial classification used derives from agricultural land soil, and the bacterial classification convenient sources is easy to cultivate, and preparation process technique is simple; The present invention can mix with chemical fertilizer use, also can be used alone, can effectively supplement the soil organism, activating soil P fertilizer and K fertilizer, improve the crop disease-resistant ability, complex microorganism particularly to preventing and treating soil-borne disease, the continuous cropping disease has effect preferably, is a kind of composite biological fertilizer of efficient, nontoxic, environmental protection; Product of the present invention is powdery, and technical indicator is: water content≤12%, total count>=2.5 * 10 6individual/g, total nutrient>=13%, total organic matter>=20%.

Embodiment

Below in conjunction with specific embodiment, the present invention is described in more detail, but the invention is not restricted to these embodiment.

the preparation process of example 1 bacterium liquid

(1) culturing process of bacillus amyloliquefaciens

the one-level test tube slant is cultivated: NYDA substratum: extractum carnis 8g, peptone 8g, glucose 10g, agar 15g, distilled water 1000mL.Bacillus amyloliquefaciens is inoculated on test tube NYDA substratum, and 28 ℃ of lower activation culture 24h obtain test tube strains.

secondary seed is cultivated: NYD substratum: extractum carnis 8g, peptone 8g, glucose 10g, distilled water 1000mL.By in above-mentioned test tube strains access NYD substratum, shaking table is cultivated 24h(28 ℃, 130 rev/mins) make primary seed solution, then primary seed solution is inoculated in 100mL NYD nutrient solution and carries out 28 ℃ of fermentation culture with the 1:100 ratio, 130 rev/mins) 24h, obtain secondary seed solution.

liquid fermentation and culture:

Adopt the BPY substratum: extractum carnis 5g, peptone 10g, NaCl 5g, yeast extract paste 5g, glucose 5g, agar, 1%-2%, distilled water 1000ml; PH=7;

After fermentation equipment and medium sterilization, the volume ratio by secondary seed solution by 1:25 is inoculated in fermentation culture, and at 28 ℃ of bottom fermentation 60h, by filtering fermentation liquor, vacuum concentration obtains the fermented liquid of bacillus amyloliquefaciens to 50% of original volume.

Sterilizing: the sterilizing of pipeline, air filter, fermentor tank, substratum is undertaken by the production technique standard.

Fermentation condition: 28 ℃ of temperature, carry out temperature regulation with the tap water circulating cooling method, tank pressure 0.05MPa; Air flow 6slpm; Stirring velocity 140rpm; Every 10h sampling 1 time, mensuration pH value, observation have or not miscellaneous bacteria etc., detect the fermentation viable count with the culture medium flat plate method and reach (1.2~2.0) * 10 8individual/mL, fermented incubation time is 60h.

(2) culturing process of subtilis

the one-level test tube slant is cultivated: subtilis is inoculated on test tube NYDA substratum, and 28 ℃ of lower activation culture 24h obtain test tube strains.

secondary seed is cultivated: by above-mentioned test tube strains access NYD substratum, shaking table is cultivated 24h(28 ℃, 130 rev/mins) make primary seed solution, then primary seed solution is inoculated in 100mL NYD nutrient solution and carries out 28 ℃ of fermentation culture with the 1:100 ratio, 130 rev/mins) 24h, obtain secondary seed solution.

liquid fermentation and culture:

After fermentation equipment and medium sterilization, the volume ratio by secondary seed solution by 1:25 is inoculated in the BPY fermentation culture, and at 30 ℃ of bottom fermentation 60h, by filtering fermentation liquor, vacuum concentration obtains the fermented liquid of subtilis to 50% of original volume.

Sterilizing: the sterilizing of pipeline, air filter, fermentor tank, substratum is undertaken by the production technique standard.

Fermentation condition: 30 ℃ of temperature, carry out temperature regulation with the tap water circulating cooling method, tank pressure 0.05MPa; Air flow 6slpm; Stirring velocity 140rpm; Every 10h sampling 1 time, mensuration pH value, observation have or not miscellaneous bacteria etc., with the culture medium flat plate method, detect fermentation viable count (2.0~2.5) * 10 8individual/mL, fermented incubation time is 60h.

(3) culturing process of Trichoderma

the one-level test tube slant is cultivated: the PDA substratum: peeling potato 200g, and, glucose 15g, agar 15g, distilled water 1000mL.Trichoderma is inoculated on test tube PDA substratum, and 28 ℃ of lower activation culture obtain test tube strains in 4 days.

secondary seed is cultivated: the PDB substratum: peeling potato 200g, and, glucose 15g, distilled water 1000mL.: by above-mentioned test tube strains access PDB substratum, shaking table is cultivated 4 days (28 ℃, 140 rev/mins) and is made seed liquor.

liquid fermentation and culture:

After fermentation equipment and medium sterilization, the volume ratio by secondary seed solution by 1:10 is inoculated in the PDB fermentation culture, at 28 ℃ of bottom fermentation 72h, filtering fermentation liquor is obtained to the fermented liquid of Trichoderma.

Sterilizing: the sterilizing of pipeline, air filter, fermentor tank, substratum is undertaken by the production technique standard.

Fermentation condition: 28 ℃ of temperature, carry out temperature regulation with the tap water circulating cooling method, tank pressure 0.05MPa; Air flow 6slpm; Stirring velocity 150rpm; Every 10h sampling 1 time, mensuration pH value, observation have or not miscellaneous bacteria etc., detect the fermentation viable count with the culture medium flat plate method and reach (2.5~3.0) * 10 8individual/mL, fermented incubation time is 72h.

Microbial inoculum required for the present invention is also commercially available.

embodiment 2

The biological compound organic fungi-manure of the present embodiment is comprised of compound manure and mixing microorganisms microbial inoculum, contains following material fertilizer in the described compound manure of every 1kg: humic acids 150g, charcoal 400g, chemical fertilizer 340g, wilkinite 110g; In every 1kg compound manure, coupling is added 15mL mixing microorganisms microbial inoculum; Described chemical fertilizer weight ratio of constituents is urea: secondary calcium phosphate: vitriolate of tartar=45:40:15; Described mixing microorganisms microbial inoculum comprises bacillus amyloliquefaciens (1.2~2.0) * 10 8individual/mL, subtilis (2.0~2.5) * 10 8individual/mL and Trichoderma (2.5~3.0) * 10 8individual/tri-kinds of mL microbial inoculum, its volume ratio is bacillus amyloliquefaciens: subtilis: Trichoderma=40:35:30.

Biological compound organic fungi-manure is prepared from by following steps:

1) preparation of mixing microorganisms microbial inoculum: bacillus amyloliquefaciens, subtilis and three kinds of microbial inoculums of Trichoderma are mixed by described volume ratio;

2) preparation of chemical fertilizer: be equipped with urea, secondary calcium phosphate and vitriolate of tartar by described weight ratio, and mix;

3) preparation of charcoal: will drop into after crops stalk crushing in the charcoal high-temperature cracking furnace, under anoxia condition, process the generation charcoal for 400 ℃, and then pulverize, cross 200~300 mesh sieves and process, stand-by;

4) preparation of compound manure: the humic acids, charcoal, chemical fertilizer, the wilkinite that have been equipped with are mixed by weight, and stir;

5) spray in proportion described mixing microorganisms microbial inoculum in described compound manure, after evenly mixing, obtain biological compound organic fungi-manure; Guarantee that in the sprinkling process microbial function bacterium that falls into stirrer keeps vaporific, every sprinkling 3 minutes, stop spraying 30 seconds, so repeat to spray 5 times, after sprinkling mixes, then send into drying installation under 40 ℃ dry 30 minutes, sieve after cooling after pulverizing and obtain function yeast number>=2.5 * 10 6individual/g, N, P, K content difference>=5%, 5%, 4%, total nutrient>=13%, the composite biological fertilizer of water content≤12%.

embodiment 3

The biological compound organic fungi-manure of the present embodiment is comprised of compound manure and mixing microorganisms microbial inoculum, contains following material fertilizer in the described compound manure of every 1kg: humic acids 250g, charcoal 340g, chemical fertilizer 300g, wilkinite 110g; In every 1kg compound manure, coupling is added 20mL mixing microorganisms microbial inoculum; Described chemical fertilizer weight ratio of constituents is urea: secondary calcium phosphate: vitriolate of tartar=45:45:10; Described mixing microorganisms microbial inoculum comprises bacillus amyloliquefaciens (1.2~2.0) * 10 8individual/mL, subtilis (2.0~2.5) * 10 8individual/mL and Trichoderma (2.5~3.0) * 10 8individual/tri-kinds of mL microbial inoculum, its volume ratio is bacillus amyloliquefaciens: subtilis: Trichoderma=30:45:25.

The preparation method is identical with embodiment 2.

embodiment 4

The biological compound organic fungi-manure of the present embodiment is comprised of compound manure and mixing microorganisms microbial inoculum, contains following material fertilizer in the described compound manure of every 1kg: humic acids 200g, charcoal 350g, chemical fertilizer 330g, wilkinite 120g; In every 1kg compound manure, coupling is added 18.5mL mixing microorganisms microbial inoculum; Described chemical fertilizer weight ratio of constituents is urea: secondary calcium phosphate: vitriolate of tartar=40:40:20; Described mixing microorganisms microbial inoculum comprises bacillus amyloliquefaciens (1.2~2.0) * 10 8individual/mL, subtilis (2.0~2.5) * 10 8individual/mL and Trichoderma (2.5~3.0) * 10 8individual/tri-kinds of mL microbial inoculum, its volume ratio is bacillus amyloliquefaciens: subtilis: Trichoderma=35:40:35.

The preparation method is identical with embodiment 2.

embodiment 5

The biological compound organic fungi-manure of the present embodiment is comprised of compound manure and mixing microorganisms microbial inoculum, contains following material fertilizer in the described compound manure of every 1kg: humic acids 150g, charcoal 350g, chemical fertilizer 350g, wilkinite 150g; In every 1kg compound manure, coupling is added 17mL mixing microorganisms microbial inoculum; Described chemical fertilizer weight ratio of constituents is urea: secondary calcium phosphate: vitriolate of tartar=40:45:15; Described mixing microorganisms microbial inoculum comprises bacillus amyloliquefaciens (1.2~2.0) * 10 8individual/mL, subtilis (2.0~2.5) * 10 8individual/mL and Trichoderma (2.5~3.0) * 10 8individual/tri-kinds of mL microbial inoculum, its volume ratio is bacillus amyloliquefaciens: subtilis: Trichoderma=35:35:30.

The preparation method is identical with embodiment 2.

embodiment 6

The biological compound organic fungi-manure of the present embodiment is comprised of compound manure and mixing microorganisms microbial inoculum, contains following material fertilizer in the described compound manure of every 1kg: humic acids 200g, charcoal 450g, chemical fertilizer 350g, wilkinite 150g; In every 1kg compound manure, coupling is added 19mL mixing microorganisms microbial inoculum; Described chemical fertilizer weight ratio of constituents is urea: secondary calcium phosphate: vitriolate of tartar=45:35:20; Described mixing microorganisms microbial inoculum comprises bacillus amyloliquefaciens (1.2~2.0) * 10 8individual/mL, subtilis (2.0~2.5) * 10 8individual/mL and Trichoderma (2.5~3.0) * 10 8individual/tri-kinds of mL microbial inoculum, its volume ratio is bacillus amyloliquefaciens: subtilis: Trichoderma=30:35:35.

The preparation method is identical with embodiment 2.

example 7 field effect tests

(1) experimental field situation and time

Situation experimental field: place is located at test base, Yuanyang County, Henan Province, within 2010, carries out the wheat field test, within 2012, carries out the sesame field test, and each test plot cultivated area is 1 mu, and cultivation management is all planted by local normal cropping pattern.

Test period: year June in October, 2010 to 2011; On September 4,9 days to 2012 June in 2012.

(2) test medicine and test kind

Test medicine: adopt example 2 to the biological compound organic fungi-manure of example 6.

The test kind: all wheat 18(wheats), Henan sesame 11(sesame).

(3) test is processed

The mixed fertilizer of the compound organic fungi-manure of above-mentioned biology and inorganic compounding carries out field comparison test under field conditions (factors), test is carried out at wheat paddock and sesame Tanaka, test examples 2 is to the bacterial manure proportioning of example 6, with current active constituent content commonly used >=45%, the common inorganic composite fertilizer that proportioning is N:P:K=15:15:15 is contrast; Cultivation management is all by normal cropping pattern plantation.

(4) investigation method

Soil-borne disease is carried out at wheat and sesame growth in season, and investigation adopts 5 methods, and every some investigation 100 strains, then calculate average attack rate, calculates prevention effect; At when results 5 method sampling survey thousand seed weight, output, each sampling spot area 5m 2, finally calculate theoretical per mu yield and effect of increasing production.

(5) test-results

Use the wheat of product of the present invention and the more common inorganic composite fertilizer of sesame processes seedling stage and whole growth phase growing way is sturdy, well developed root system, and can effectively prevent and treat root disease, improve output.

Wheat paddock result (table 1) shows: wheat root disease sickness rate contrasts average decline 12.77%~26.6%, and output contrasts average raising 6.76%~8.22%.

Sesame field result (table 2) shows: the sesame root disease descends 8.04%~20.76%, the highest raising 7.13% of mean yield.

The present invention is not limited to above-mentioned embodiment, and those skilled in the art also can make multiple variation accordingly, but any and the present invention are equal to or similarly change and all should be encompassed in the scope of the claims in the present invention.

Claims (3)

1. the compound organic fungi-manure of biology, the compound organic fungi-manure of described biology is comprised of compound manure and mixing microorganisms microbial inoculum, it is characterized in that, contain the material fertilizer of following weight percent in described compound manure: humic acid 14.5%~25.2%, charcoal 33.5%~45.1%, chemical fertilizer 22.5%~35.5%, wilkinite 10.2%~15.4%; In addition, add 10mL~20mL mixing microorganisms microbial inoculum in every 1kg compound manure;
Described chemical fertilizer composition is urea, secondary calcium phosphate and vitriolate of tartar, and its weight ratio is urea: secondary calcium phosphate: vitriolate of tartar=(40~45): (35~45): (10~20); Contain bacillus amyloliquefaciens, subtilis and three kinds of microbial inoculums of Trichoderma in described mixing microorganisms microbial inoculum, its volume ratio is bacillus amyloliquefaciens: subtilis: Trichoderma=(30~40): (35~45): (25~35).
2. biological compound organic fungi-manure according to claim 1, is characterized in that: described bacillus amyloliquefaciens (1.2~2.0) * 10 8individual/mL, subtilis (2.0~2.5) * 10 8individual/mL, Trichoderma (2.5~3.0) * 10 8individual/mL.
3. according to the compound organic fungi-manure of the described biology of claim 1 ~ 2 any one, it is characterized in that, be prepared from by following steps:
(1) preparation of mixing microorganisms microbial inoculum: bacillus amyloliquefaciens, subtilis and three kinds of microbial inoculums of Trichoderma are mixed by described volume ratio;
(2) preparation of chemical fertilizer: be equipped with urea, secondary calcium phosphate and vitriolate of tartar by described weight ratio, and mix;
(3) preparation of charcoal: will drop into after crops stalk crushing in the charcoal high-temperature cracking furnace, under anoxia condition, process the generation charcoal for 300 ℃~500 ℃, and then pulverize, cross 200~300 mesh sieves and process, stand-by;
(4) preparation of compound manure: the humic acids, charcoal, chemical fertilizer, the wilkinite that have been equipped with are mixed by weight, and stir;
(5) spray in proportion described mixing microorganisms microbial inoculum in described compound manure, after evenly mixing, obtain biological compound organic fungi-manure.
CN201310371885.1A 2013-08-23 2013-08-23 Biological compound organic bacterial fertilizer CN103467185B (en)

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