CN102037964B - Application of farrerol in preparing bactericides - Google Patents
Application of farrerol in preparing bactericides Download PDFInfo
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- CN102037964B CN102037964B CN201110004252.8A CN201110004252A CN102037964B CN 102037964 B CN102037964 B CN 102037964B CN 201110004252 A CN201110004252 A CN 201110004252A CN 102037964 B CN102037964 B CN 102037964B
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- pathogenic fungi
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Abstract
The invention discloses an application of farrerol in preparing bactericides. The natural compound provided by the invention refers to farrerol, and relevant tests show that the farrerol can effectively kill plant pathogenic fungi, therefore, the farrerol is a most promising raw material which can be used for preparing drugs for killing or inhibiting plant pathogenic fungi.
Description
Technical field
The present invention relates to a kind of purposes of native compound, the present invention is exactly the application of farrerol in preparing bactericide.
Background technology
Plant pathogenic fungi is harm plant growth, causes a kind of major reason of the plant underproduction, is also the storage that affects plant seed kind, stem, leaf simultaneously, and then affects the key factor of food security.The plant pathogenic fungi medicine of killing of present stage chemosynthesis plays not replaceable effect in control of plant disease.But the public more and more payes attention to the pollution problem of environment toxicity of pesticide and agricultural chemicals, some toxicity are high, and the bactericide kind of residual length, as organic mercurials and difoltan etc. are forbidden in succession.After China joined WTO, residue of pesticide have become the serious hindrance of China's agricultural production and the export of farm produce especially.From organism, particularly find new efficient, low toxicity, sterilization or Substance that low-residual is good with Environmental compatibility plant corpus, become the focus that current novel pesticide is researched and developed.
Plant is the natural treasure-house of bioactive compound, and its secondary metabolite surpasses 400,000 kinds, wherein, many times living beings have desinsection (or) sterilization, the biologically active such as antibacterial, in agricultural, pharmaceutically have an important researching value.The compound of vegetalitas or plant resource is just widely used since ancient times on the mankind's medicine and agricultural chemicals.Most important three major types insecticide: pyrethroid, carbamates and the anabasine developing rapidly in recent years, its synthetic lead compound is all from higher plant; And, rotenone, different goat's horn is turned round all commercializations of many new vegetable insecticide such as glucoside and nimbin.1998, Meng Zhaoli etc. isolate from ginkgo had very highly active compound to plant pathogenic fungi, developed " green Supreme Being " agricultural bactericidal agent series products.Because botanical pesticide is from natural, there is easy biological degradation, the advantage such as free from environmental pollution.At present, finding antimicrobial compound and develop the attention that Plant Fungicide is just more and more subject to countries in the world from plant, is also the operative orientation that antibacterials R&D and production unit weight is wanted.
Summary of the invention
The invention provides a kind of native compound and as preparation, kill the medicine of plant pathogenic fungi.
The native compound of indication of the present invention is farrerol, according to relevant test, shows, farrerol can effectively be killed plant pathogenic fungi, is a kind of extremely promising raw material that the medicine of plant pathogenic fungi was killed or suppressed in preparation that can be used for.
Farrerol belongs to flavanone material, is to obtain from the acrial part separation of Hainan cuckoo.The research of farrerol has had the history of decades, is mainly used in early days chronic bronchitis and antibacterial action, and clinical practice is still mainly confined to phlegm-dispelling functions.Existing document has farrerol for antineoplastic content, referring to Chinese invention patent application 200910074512.1, up to the present, whether has the research that suppresses plant pathogenic fungi growth and kill plant pathogenic fungi also there is no bibliographical information about farrerol.In addition, have in the prior art the extracting method of multiple farrerol, for example Chinese invention patent application 200910032992.5 discloses a kind of extracting method of farrerol.
Of the present invention experimental results show that: farrerol is for various plants disease fungus as lower fungus, and higher fungi has very strong in vitro antibacterial activity, shows wider fungicidal spectrum; Farrerol especially has good bacteriostasis to withered germ of water-melon, even better than the fungistatic effect of the methyl Tobe of positive control, and to plant without poisoning.Research and experiment shows, the bacteriostasis mode of farrerol and mechanism are to cause watermelon blight fungal pathogens silk deformity: branch increases, and mycelia attenuates, and mycelia hollow or split has the effect of good killing fungus.
The present invention has following beneficial effect:
(1) farrerol is natural antibacterial compound, nontoxic, has no side effect, free from environmental pollution;
(2) farrerol has broad spectrum activity, and bactericidal activity is high, and usage amount is low, and protection effect is remarkable;
(3) bactericidal action mechanism is unique, contributes to administer the in fact serious pesticide resistance problem of bactericide of producing;
(4) farrerol is natural products, and can industry synthesize.The structure of this compound of can also take is from now on template, carries out molecular structure alteration, studies its structure-activity relationship, and expectation obtains more highly active compound, and is developed to new bactericide kind.
Embodiment
By specific embodiments of the invention given below, can further be expressly understood invention, but following embodiment not limitation of the invention.
The bacteriostatic activity of embodiment 1 farrerol to plant pathogenic fungi mycelial growth
For examination disease fungus banana blight bacteria (F.oxysporum f.sp.cubense race 4), Colletotrichum gloeosporioides Penz in Mango (C.gloeosporioids), cucumber anthracnose (Phytophthora melonis), Penicillium italicum bacterium (Penicillium italicum), Rhizoctonia solani Kuhn (Rhizoctonia solani), withered germ of water-melon (Fusarium oxysporum f.sp.Niveum).
Experimental technique adopts the growth rate method of toxic medium to measure the inhibitory action of farrerol to plant pathogenic fungi mycelial growth.Summary for by farrerol with joining after acetone solution in the PDA medium of melting (content of acetone 0.4%), be made into respectively containing the certain density pastille PDA of farrerol dull and stereotyped.By cultivating diameter activate, to be that the mycelia piece of each disease fungus of 5mm moves on to pastille flat board central, and each concentration is established three repetitions, and establish 0.4% acetone in contrast.Put 25 ℃ of constant temperature culture.By right-angled intersection method, measure colony diameter, and proofread and correct, calculate antibacterial percentage, calculate virulence regression equation y=a+bx, concentration (EC in inhibition
50).
Experimental result
Farrerol to the inhibitory action test result of plant pathogenic fungi mycelial growth in Table.Farrerol all shows good bacteriostatic activity to the mycelial growth of the various plants disease fungus for examination as seen from Table 1.Wherein to concentration EC in the inhibition of withered germ of water-melon
50value is 9 μ g/mL, even active better than the inhibition of positive control thiophanate methyl.To concentration EC in the inhibition of Colletotrichum gloeosporioides Penz in Mango
50be 18 μ g/mL.
Inhibitory action (the EC of table 1 farrerol to plant pathogenic fungi
50, μ g/mL)
Experimental example 2 farrerols are observed withered germ of water-melon bacteriostasis mechanism
Examination is for disease fungus withered germ of water-melon
Test method:
Farrerol is made to the toxic flat board of 10 μ g/mL, on toxic flat board, cultivate watermelon blight fungal pathogens, do control experiment picking mycelia simultaneously, under light microscope, observe the form of mycelia.
Result of the test
The bacterium colony of the watermelon blight fungal pathogens on the toxic flat board of farrerol is abnormal.Under light microscope, the obvious form of visible mycelia deformity: show as branch and increase, mycelia attenuates, mycelia hollow, the phenomenon such as split.
Claims (1)
1. farrerol application in the medicine of preparing Suppressing phytopathogens withered germ of water-melon as unique active component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110004252.8A CN102037964B (en) | 2011-01-06 | 2011-01-06 | Application of farrerol in preparing bactericides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110004252.8A CN102037964B (en) | 2011-01-06 | 2011-01-06 | Application of farrerol in preparing bactericides |
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| Publication Number | Publication Date |
|---|---|
| CN102037964A CN102037964A (en) | 2011-05-04 |
| CN102037964B true CN102037964B (en) | 2014-01-22 |
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| CN201110004252.8A Expired - Fee Related CN102037964B (en) | 2011-01-06 | 2011-01-06 | Application of farrerol in preparing bactericides |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614589A (en) * | 2016-11-13 | 2017-05-10 | 徐州得铸生物科技有限公司 | Insecticide composition containing tribulus terrestris saponins |
| CN108576083A (en) * | 2018-07-27 | 2018-09-28 | 贵州省茗之源种植专业合作社 | A kind of Chinese medicine composition and preparation method thereof preventing cucumber anthracnose |
| CN108640768A (en) * | 2018-07-27 | 2018-10-12 | 普定县景凤种养殖专业合作社 | A kind of fertilizer and preparation method thereof of prevention cucumber anthracnose |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1111478A (en) * | 1994-11-01 | 1995-11-15 | 吴日章 | Insectiside composed of Chinese herbal medicines and preparing method thereof |
| CN1293900A (en) * | 2000-11-29 | 2001-05-09 | 武剑辉 | Aquatic stemonine solution as plant insecticide |
| CN1493195A (en) * | 2003-09-12 | 2004-05-05 | 李瑞金 | Insecticide, bactericide and its preparation and application method |
| CN1618293A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | Non-pollution agricultural chemicals, and prepn. method therefor |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1909559A4 (en) * | 2005-07-08 | 2009-01-21 | Hexima Ltd | Management of plant pathogens |
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2011
- 2011-01-06 CN CN201110004252.8A patent/CN102037964B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1111478A (en) * | 1994-11-01 | 1995-11-15 | 吴日章 | Insectiside composed of Chinese herbal medicines and preparing method thereof |
| CN1293900A (en) * | 2000-11-29 | 2001-05-09 | 武剑辉 | Aquatic stemonine solution as plant insecticide |
| CN1493195A (en) * | 2003-09-12 | 2004-05-05 | 李瑞金 | Insecticide, bactericide and its preparation and application method |
| CN1618293A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | Non-pollution agricultural chemicals, and prepn. method therefor |
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| CN102037964A (en) | 2011-05-04 |
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Granted publication date: 20140122 Termination date: 20170106 |