CN103300069A - Biological bactericide composition and application thereof to wilt - Google Patents
Biological bactericide composition and application thereof to wilt Download PDFInfo
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- CN103300069A CN103300069A CN2013101961548A CN201310196154A CN103300069A CN 103300069 A CN103300069 A CN 103300069A CN 2013101961548 A CN2013101961548 A CN 2013101961548A CN 201310196154 A CN201310196154 A CN 201310196154A CN 103300069 A CN103300069 A CN 103300069A
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- bactericide composition
- biological bactericide
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- bacillus subtilis
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Abstract
The invention relates to a biological bactericide composition and an application thereof to a wilt. The biological bactericide composition comprises active components including amino-oligosaccharin and bacillus subtilis, wherein the weight content of the amino-oligosaccharin is 0.5-5%; the content of the bacillus subtilis is 1.0-1,000.0 hundred million active spores per gram. The biological bactericide composition disclosed by the invention is testified by a field trial and has good prevention and treatment effects on watermelon and melon wilts; meanwhile, all the active components are compounded to further improve the prevention effect and the prevention effect is obviously better than that of a single-dosage variety.
Description
Technical field
The present invention relates to a kind of biological bactericide composition and the application on fusarium wilt thereof.
Background technology
Bacillus subtilis is a kind of have a liking for temperature, aerobic, sporiferous bacillus, and its physiological characteristic is various, and is widely distributed, very easily separates and cultivates.This bacterium is widespread in nature, and is nontoxic to people and animals, free from environmental pollution, can produce multiple antibiotic and enzyme, has broad spectrum antibiotic activity and extremely strong anti-adversity ability.Bacillus subtilis not only can be in the external environments such as soil, plant rhizosphere body surface extensively exists, and is common endogenetic bacteria in the plant corpus, especially in root, the stem of plant.This bacterium demonstrates good disease-controlling effect crops such as paddy rice, cotton, capsicum, tomato flowers at present.The prominent features such as growth is fast because bacillus subtilis has, nutrition is simple and it is heat-resisting to produce, degeneration-resistant gemma, producing, formulation and the survival in environment, surely growing and breeding and make it not only be conducive to biocontrol fungicide, and mass production processes is simple, cost is lower, use conveniently, storage life is long, has now become a kind of desirable biocontrol bacteria.
Amino-oligosaccharide (chitosan oligosaccharide) is the further product of degraded of chitosan, shitosan is a kind of polysaccharose boiomacromolecule, be widespread in nature in the unicellular lower eukaryote mushroom, the cell of algae, the shell of a joint animal shrimp, crab, insect, inner casing and the cartilage of mollusk (such as squid, cuttlefish), the cell wall of higher plant etc., the synthetic resource of annual life can reach 2,000 hundred million tons, being the second largest living resources that are only second to vegetable fiber on the earth, is human inexhaustible living resources.
Amino-oligosaccharide (agricultural level chitosan oligosaccharide) can produce inhibitory action to the growth of some germs, affect fungus spore germination, brings out that the mycelia form morphs, the interior biochemistry of spore changes etc.Can excite the plant vivo gene, produce and to have chitinase, dextranase, guarantor's element and the PR albumen etc. of resistant effect, and have the cell activation effect, the recovery of the plant that helps to be injured strengthens the resistance of crop at short root strong sprout, and Promoting plant growth is grown.The amino-oligosacchride cellulose solution has virus killing, kills bacterium, fungicidal action.Not only fungi, bacterium, virus are had extremely strong control and eradicant action, but also have nutrition, adjusting, detoxifcation, antibiotic effect.Can be widely used in the diseases such as virus, bacterium, fungus-caused mosaic disease, little leaf, spot disease, anthracnose, downy mildew, epidemic disease, climing rot, verticillium wilt, rice blast, bacterial wilt, soft rot of control fruit tree, vegetables, underground rhizome, tobacco, traditional Chinese medicine and grain and cotton crop.
Induce the sterilization pesticide chitosan oligosaccharide with advantages such as its wide material sources, the growths of the high also energy of induce resistance coordinate plant growth, become gradually the domestic and international focus of attention.As biopesticide, chitosan oligosaccharide diseases prevention and disease-resistant aspect number of mechanisms is arranged, except as active signaling molecule, excite rapidly the defense response of plant, start system of defense, make plant produce the disease-resistance substances such as phenolic compound, lignin, protective plant protecting agent, pathogenesis-related proteins, and the raising defensive ferment relevant with disease-resistant metabolism and the Active oxygen eliminating enzyme system activity of uniting, oligosaccharides also is its disease-resistant necessary component to the direct inhibitory action of phytopathogen.
At present, the amino-oligosaccharide formulation products mainly contains 1%, 2%, 3% and 5% amino-oligosaccharide aqua etc., is mainly used in vegetables, fruit tree prevents and treats the effects such as virus disease, late blight and growth regulation, enhancing resistance.But its unitary agent is obvious not as the chemical agent preventive effect because of bactericidal effect, do not accepted and pay attention to by the producer, therefore extensive not on the purposes on producing, do not give full play to the effect of amino-oligosaccharide, the bacillus subtilis action effect is different because of applying environment, and effect is stable not.By to carry out science composite from different pharmacy varieties, be the control germ and improve preventive effect, the convenient effective way of using.The relevant built agent kind of amino-oligosaccharide and bacillus subtilis is all less at present, can not satisfy the needs of producing upper crops control of crop disease.
West (sweet) cucurbit wilt is a kind of fungoid soil-borne disease, fusarium wilt: be commonly called as dead arm, wilt disease, its cause of disease is Fusarium oxysporum, can fall ill whole breeding time, and knot melon phase and to expand the later stage be two peaks has a strong impact on the yield and quality of west (sweet) melon.During morbidity, the jaundice of plant lower blade is upwards development gradually, and wilt daytime, sooner or later recovers, and is dead after several days.Germ survives the winter in soil or the fertilizer that carries disease germs, the vitality extra-heavy; The 5-6 of can in soil, surviving, by seed, muck, the propagation such as flowing water, germ is invaded from the root wound, also can invade on root hair top, grows in conduit, excretes poison, and the filling conduit causes that plant is dead.General occurrence factor is, hypsography low-lying, and impeded drainage, base fertilizer is not enough, and nitrogenous fertilizer is excessive, and broad irrigation waits between continuous cropping continuous cropping or the acid-base value 4-6 all can cause the disease generation or increase the weight of.At present in the world, there is not good medicament to administer this disease.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of watermelon and Muskmelon Fusarium wilt are had the biological bactericide composition of better control efficiency and the application on fusarium wilt thereof.
Biological bactericide composition of the present invention, its active ingredient is: amino-oligosaccharide and bacillus subtilis, amino-oligosaccharide weight content are 0.5～5%, the bacillus subtilis bacterial content is 1.0～1000.0 hundred million spore alive/grams.Biological bactericide composition of the present invention is wetting powder or the aqua that the conventional machining method is processed into.
Biological bactericide composition of the present invention is mainly used in preventing and treating watermelon or Muskmelon Fusarium wilt.
Biological bactericide composition of the present invention proves through field trial, and it has good control efficiency to watermelon and Muskmelon Fusarium wilt; Pass through simultaneously the composite of each active ingredient, can further improve preventive effect, preventive effect is significantly better than the single dose kind.
Embodiment
Embodiment 1
With amino-oligosaccharide (rolling over hundred weight) 10 grams, 2,000 hundred million bacillus subtilises 100 that live spore/gram restrain, lauryl sodium sulfate 30 grams, dispersing agent NNO 40 grams, white carbon 50 grams, the kaolin surplus adds to 1000 grams, mixture carries out air-flow crushing, makes the wetting powder of amino-oligosacchride cellulose content 1%, bacillus subtilis bacterial content 20,000,000,000/gram.
Embodiment 2
With amino-oligosaccharide (rolling over hundred weight) 20 grams, 2,000 hundred million spores alive/gram bacillus subtilis 20 grams, lauryl sodium sulfate 70 grams, dispersing agent NNO 90 grams, white carbon 120 grams, the kaolin surplus adds to 1000 grams, mixture carries out air-flow crushing, makes the wetting powder of amino-oligosacchride cellulose content 2%, bacillus subtilis bacterial content 4,000,000,000/gram.
Embodiment 3
With amino-oligosaccharide (rolling over hundred weight) 5 grams, 2,000 hundred million bacillus subtilises 200 that live spore/gram restrain, and directly add deionized water, complement to 1000 grams, utilize high-shear homogenizer to stir after the filtration, make the aqua of amino-oligosacchride cellulose content 0.5%, bacillus subtilis bacterial content 40,000,000,000/gram.
Embodiment 4
With amino-oligosaccharide (rolling over hundred weight) 15 grams, 2,000 hundred million bacillus subtilises 10 that live spore/gram restrain, and directly add deionized water, complement to 1000 grams, utilize high-shear homogenizer to stir after the filtration, make the aqua of amino-oligosacchride cellulose content 1.5%, bacillus subtilis bacterial content 2,000,000,000/gram.
Prevent and treat respectively watermelon and Muskmelon Fusarium wilt test with the biological bactericide composition that above-mentioned example makes.
Test and process: by the preparation that the prescription of implementing in 1～4 is made, all adopt respectively 2 concentration sprayings, the contrast medicament is commercially available 1,000 hundred million spore alive/gram bacillus subtilis wetting powder and 3% amino-oligosaccharide aqua and clear water blanks.Prevent and treat respectively watermelon and Muskmelon Fusarium wilt.Test is to be respectively the their early stage that watermelon and Muskmelon Fusarium wilt field begin to have reveal any symptoms period, and two heavier fields of fusarium wilt occured upper one year.Two repetitions are all established respectively in test, watermelon and the every residential quarter of muskmelon field trial are 20 strains, every a Zhou Zaiyong once, and twice totally, behind twice medicine 30 days according to country's general investigation grade scale investigation and computational methods, calculate respectively the average preventive effect of watermelon and muskmelon (seeing Table).
Table: the embodiment of the invention is to the field control effect of watermelon and Muskmelon Fusarium wilt
Claims (3)
1. biological bactericide composition, it is characterized in that: its active ingredient is amino-oligosaccharide and bacillus subtilis, and the amino-oligosaccharide weight content is 0.5～5%, and the bacillus subtilis bacterial content is 1.0～1000.0 hundred million spore/grams of living.
2. biological bactericide composition according to claim 1 is characterized in that: wetting powder or aqua that it is processed into for the conventional machining method.
3. the application of the biological bactericide composition of claim 1 on control watermelon or Muskmelon Fusarium wilt.
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| CN2013101961548A CN103300069A (en) | 2013-05-22 | 2013-05-22 | Biological bactericide composition and application thereof to wilt |
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| CN2013101961548A CN103300069A (en) | 2013-05-22 | 2013-05-22 | Biological bactericide composition and application thereof to wilt |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103739354A (en) * | 2013-12-14 | 2014-04-23 | 王龙潮 | Peanut biological wettable powder fertilizer and preparation method thereof |
| CN105340641A (en) * | 2015-11-18 | 2016-02-24 | 北京联合大学 | Pesticide applying method using biopesticide to control peach tree insect disease in whole process |
| CN105660704A (en) * | 2016-01-07 | 2016-06-15 | 海南利蒙特生物农药有限公司 | Bacillus subtilis and oligosaccharins compound composition, compound preparation and applications of compound composition and compound preparation |
| CN107445741A (en) * | 2017-09-28 | 2017-12-08 | 惠州市欧野科技有限公司 | A kind of watermelon mill water culture nutrient solution |
| CN107593770A (en) * | 2017-08-25 | 2018-01-19 | 河南中麦生物科技有限公司 | A kind of microbial inoculum and its application for being used to set withered retrogradation greatly |
| CN107821439A (en) * | 2017-10-27 | 2018-03-23 | 沈阳化工研究院有限公司 | A kind of composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN108244139A (en) * | 2018-03-19 | 2018-07-06 | 联保作物科技有限公司 | Complex composition, soil conditioner and its application containing biostimulant and microbial bacterial agent |
| CN109820005A (en) * | 2019-03-21 | 2019-05-31 | 天津市农业资源与环境研究所 | A kind of antibacterial insect prevention ecological system formulation of biology and the preparation method and application thereof |
| CN110367283A (en) * | 2019-08-08 | 2019-10-25 | 咸阳润源生物科技有限公司 | A kind of complex micro organism fungicide and its application |
| CN110668891A (en) * | 2019-09-25 | 2020-01-10 | 山东宝源生物科技股份有限公司 | Biocontrol type compound additive and application thereof in fertilizer |
| CN112493255A (en) * | 2020-12-02 | 2021-03-16 | 青岛博智汇力生物科技有限公司 | Plant growth regulator containing chitosan oligosaccharide |
| CN112674115A (en) * | 2021-01-21 | 2021-04-20 | 山东亿嘉农化有限公司 | Compound preparation for preventing and treating ginger stem rot and application thereof |
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| CN1930991A (en) * | 2006-10-01 | 2007-03-21 | 黄高 | Re-compounded germicide containing Bucillus licheniformis |
| CN101124916A (en) * | 2007-08-06 | 2008-02-20 | 大连奥德植保药业有限公司 | Biological pesticide mixed with plant insecticide sterilization component and amino-oligosaccharin and preparation method thereof |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103739354A (en) * | 2013-12-14 | 2014-04-23 | 王龙潮 | Peanut biological wettable powder fertilizer and preparation method thereof |
| CN103739354B (en) * | 2013-12-14 | 2016-04-27 | 王龙潮 | A kind of Peanut biological wettable powder fertilizer and preparation method thereof |
| CN105340641A (en) * | 2015-11-18 | 2016-02-24 | 北京联合大学 | Pesticide applying method using biopesticide to control peach tree insect disease in whole process |
| CN105660704A (en) * | 2016-01-07 | 2016-06-15 | 海南利蒙特生物农药有限公司 | Bacillus subtilis and oligosaccharins compound composition, compound preparation and applications of compound composition and compound preparation |
| CN107593770A (en) * | 2017-08-25 | 2018-01-19 | 河南中麦生物科技有限公司 | A kind of microbial inoculum and its application for being used to set withered retrogradation greatly |
| CN107445741A (en) * | 2017-09-28 | 2017-12-08 | 惠州市欧野科技有限公司 | A kind of watermelon mill water culture nutrient solution |
| CN107821439A (en) * | 2017-10-27 | 2018-03-23 | 沈阳化工研究院有限公司 | A kind of composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN107821439B (en) * | 2017-10-27 | 2020-06-23 | 沈阳化工研究院有限公司 | Composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN108244139A (en) * | 2018-03-19 | 2018-07-06 | 联保作物科技有限公司 | Complex composition, soil conditioner and its application containing biostimulant and microbial bacterial agent |
| CN109820005A (en) * | 2019-03-21 | 2019-05-31 | 天津市农业资源与环境研究所 | A kind of antibacterial insect prevention ecological system formulation of biology and the preparation method and application thereof |
| CN110367283A (en) * | 2019-08-08 | 2019-10-25 | 咸阳润源生物科技有限公司 | A kind of complex micro organism fungicide and its application |
| CN110367283B (en) * | 2019-08-08 | 2022-06-17 | 咸阳润源生物科技有限公司 | Compound microbial agent and application thereof |
| CN110668891A (en) * | 2019-09-25 | 2020-01-10 | 山东宝源生物科技股份有限公司 | Biocontrol type compound additive and application thereof in fertilizer |
| CN112493255A (en) * | 2020-12-02 | 2021-03-16 | 青岛博智汇力生物科技有限公司 | Plant growth regulator containing chitosan oligosaccharide |
| CN112674115A (en) * | 2021-01-21 | 2021-04-20 | 山东亿嘉农化有限公司 | Compound preparation for preventing and treating ginger stem rot and application thereof |
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Application publication date: 20130918 |