CN102356771A - Sterilization composition and use thereof - Google Patents
Sterilization composition and use thereof Download PDFInfo
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- CN102356771A CN102356771A CN2011102343144A CN201110234314A CN102356771A CN 102356771 A CN102356771 A CN 102356771A CN 2011102343144 A CN2011102343144 A CN 2011102343144A CN 201110234314 A CN201110234314 A CN 201110234314A CN 102356771 A CN102356771 A CN 102356771A
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- polyoxin
- bacillus subtilis
- active component
- sterilization composition
- strawberry
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Abstract
The invention discloses a sterilization composition, and belongs to the field of agricultural bactericides. An active component of the sterilization composition comprises bacillus subtilis and polyoxin. The sterilization composition comprises 10 to 85 wt% of the active component. The active component comprises 10 to 70 wt% of bacillus subtilis. The invention also discloses a use of the sterilization composition in prevention and treatment of grey mould fruit rot of strawberry, cucumber fusarium wilt and rice sheath blight. Compared with a sterilization preparation prepared only from polyoxin or bacillus subtilis as an active component, the sterilization composition provided by the invention has better effects and activity of prevention and treatment of grey mould fruit rot of strawberry, cucumber fusarium wilt and rice sheath blight. Because of different mechanisms of effects produced by bacillus subtilis and polyoxin in the active component, polyoxin resistance is relieved and a sterilization composition service life is prolonged.
Description
Technical field
The present invention relates to a kind of agricultural bactericidal medicament and application thereof, relate in particular to a kind of bactericidal composition and application thereof.
Background technology
Along with improving constantly of fast development of national economy and living standards of the people, society has higher requirement to the quality and the safety thereof of agricultural product.A lot of crops cause damage by disease and insect easily owing to adopt the large batch of production management pattern of scale.Such as in recent years, the gray mold frequency of occurrences of strawberry is increasingly high, and disease is also serious gradually, becomes the restraining factors that strawberry produces; Cucumber, tomato establishing in large scale also fusarium wilt can occur; Rice sheath blight disease and for example, over past ten years, because high-quality, the high-yield rice kind of the late-maturing type of establishing in large scale, and climate warming and technological the promoting the use of of light cultivation, rice sheath blight disease causes harm and totally is the situation that rises significantly.According to statistics, the annual medicament that only is used to prevent and treat rice sheath blight disease reaches tons up to ten thousand, this has not only caused the ecological ring in serious paddy field to scratch and water pollution, and has directly increased the residual of toxic chemical substance in the rice, and human health is caused serious threat.Therefore, provide the safe agricultural product of good quality and high output to become the important directions of current agricultural development.
Bacillus subtilis (Bacillus subtilis) is a kind of of bacillus.Be a kind of have a liking for temperature, aerobic, sporiferous bacillus, its physiological characteristic is various, distributes extensively, very easily separation and Culture.This bacterium is widespread in nature, and is nontoxic to people and animals, free from environmental pollution, can produce multiple antibiotic and enzyme, has broad spectrum antibiotic activity and extremely strong anti-adversity ability.Bacillus subtilis can extensively exist in external environments such as soil, root system of plant, body surface, on crops such as paddy rice, cotton, capsicum, tomato, flowers, demonstrates good disease-controlling effect.Prominent features such as growth is fast because withered grass bud pole bacterium has, nutrition is simple and it is heat-resisting to produce, degeneration-resistant gemma; Making it help biocontrol fungicide is producing, formulation processing and the survival in environment, is growing and breeding surely; And mass production processes is simple, and cost is lower, uses conveniently; Storage life is long, has become a kind of desirable biocontrol bacteria at present.
Polyoxin is claimed Polyoxin again, molecular formula: C
17H
25N
5O
13, nineteen sixty-five is found by Japan the earliest, is the antibiotic with peptide pyrimidine nucleotide class formation.Its mechanism of action mainly is to disturb the chitinous biosynthesis of pathogenic cell wall, and gemma expands with part after mycelium contacts medicament and breaks, and overflows cell inclusion, causes death.Polyoxin has the effect that germ produces spore and scab expansion that suppresses.Many disease funguses in sac fungi, basidiomycetes, the Fungi Imperfecti are had higher activity, and stability and safety are good, and the market price is lower.
Summary of the invention
Goal of the invention: the purpose of this invention is to provide a kind of bactericidal composition, obtain a kind of sterilization combination medicament to grey mould fruit rot of strawberry, cucumber fusarium axysporum, rice sheath blight disease controlling better effects if; Another object of the present invention is
Technical scheme: be the realization above-mentioned purpose, a kind of bactericidal composition of the present invention, active component comprises bacillus subtilis and polyoxin, and said active component accounts for 10～85% of gross weight, and said bacillus subtilis accounts for 10～70% of active component weight.
Said bactericidal composition is a wetting powder.Except comprising active component, also comprise auxiliary agent and fill auxiliary material that wherein, auxiliary agent comprises wetting agent, dispersant, fills auxiliary material and comprises precipitated calcium carbonate.Said auxiliary agent is to prepare the common auxiliary agent that wetting powder adopted and fill auxiliary material with filling auxiliary material.
Beneficial effect: bactericidal composition provided by the invention, the control efficiency on grey mould fruit rot of strawberry, cucumber fusarium axysporum, rice sheath blight disease and active all are better than the bactericidal agent that single employing polyoxin or bacillus subtilis are mixed with as active component.Because the mechanism of action of two kinds of medicaments in the active component is different, alleviated the resistance problem of polyoxin, prolong the service life of medicament.
Embodiment
Embodiment 1
Following component is pressed the preparation of constant weight percentage:
The former medicine 7.85g of polyoxin;
Bacillus subtilis 5g;
Wetting agent K12 (lauryl sodium sulfate) 4g;
Dispersant NNO (sodium metnylene bis-naphthalene sulfonate) 8g;
Precipitated calcium carbonate complements to 100g.
Wherein, the former medicine of 32% polyoxin is provided by Wuhan Nature; 20,000,000,000 spores/gram bacillus subtilis wetting powder is provided by Wuhan Kenuo Biology Science & Technology Co., Ltd..The former medicine of polyoxin, bacillus subtilis are placed blender according to the above ratio; Add a certain proportion of wetting agent K12 and dispersant NNO; Be filled to 100% with precipitated calcium carbonate; After all materials are done and are mixed in the blender, through comminution by gas stream 2 times, 2.5% polyoxin, 10,000,000,000 bacillus subtilis wetting powders.
Embodiment 2
Following component is pressed the preparation of constant weight percentage:
The former medicine 50g of polyoxin;
Bacillus subtilis 35g;
Wetting agent K12 (lauryl sodium sulfate) 4g;
Dispersant NNO (sodium metnylene bis-naphthalene sulfonate) 8g;
Precipitated calcium carbonate complements to 100g.
Wherein, the former medicine of 32% polyoxin is provided by Wuhan Nature; 20,000,000,000 spores/gram bacillus subtilis wetting powder is provided by Wuhan Kenuo Biology Science & Technology Co., Ltd..The former medicine of polyoxin, bacillus subtilis are placed blender according to the above ratio; Add a certain proportion of wetting agent K12 and dispersant NNO; Be filled to 100% with precipitated calcium carbonate; After all materials are done and are mixed in the blender, through comminution by gas stream 2 times, 2.5% polyoxin, 10,000,000,000 bacillus subtilis wetting powders.
Embodiment 3
Following component is pressed the preparation of constant weight percentage:
The former medicine 50g of polyoxin;
Bacillus subtilis 5g;
Wetting agent K12 (lauryl sodium sulfate) 4g;
Dispersant NNO (sodium metnylene bis-naphthalene sulfonate) 8g;
Precipitated calcium carbonate complements to 100g.
Wherein, the former medicine of 32% polyoxin is provided by Wuhan Nature; 20,000,000,000 spores/gram bacillus subtilis wetting powder is provided by Wuhan Kenuo Biology Science & Technology Co., Ltd..The former medicine of polyoxin, bacillus subtilis are placed blender according to the above ratio; Add a certain proportion of wetting agent K12 and dispersant NNO; Be filled to 100% with precipitated calcium carbonate; After all materials are done and are mixed in the blender, through comminution by gas stream 2 times, 2.5% polyoxin, 10,000,000,000 bacillus subtilis wetting powders.
Embodiment 4
Following component is pressed the preparation of constant weight percentage:
The former medicine 8g of polyoxin;
Bacillus subtilis 2g;
Wetting agent K12 (lauryl sodium sulfate) 4g;
Dispersant NNO (sodium metnylene bis-naphthalene sulfonate) 8g;
Precipitated calcium carbonate complements to 100g.
Wherein, the former medicine of 32% polyoxin is provided by Wuhan Nature; 20,000,000,000 spores/gram bacillus subtilis wetting powder is provided by Wuhan Kenuo Biology Science & Technology Co., Ltd..The former medicine of polyoxin, bacillus subtilis are placed blender according to the above ratio; Add a certain proportion of wetting agent K12 and dispersant NNO; Be filled to 100% with precipitated calcium carbonate; After all materials are done and are mixed in the blender, through comminution by gas stream 2 times, 2.5% polyoxin, 10,000,000,000 bacillus subtilis wetting powders.
Embodiment 5
Scrape in the field and to get ash arrhizus bacteria, be made into conidial suspension with sterile water, will there be 10～20 conidiums in suspension concentration each visual field under 10 * 10 power microscopes.2.5% polyoxin, the 10000000000 bacillus subtilis wetting powders of getting embodiment 1 are as test medicine (group A); Contrast agents is respectively the 3% polyoxin wetting powder that Wuhan Nature provides (group B), and 20,000,000,000 spores/gram bacillus subtilis wetting powder (organizing C) of providing of Wuhan Kenuo Biology Science & Technology Co., Ltd..
3 groups of soups of even spraying different disposal concentration on the fresh strawberry plant leaf, behind the 24h on every blade drop 3 points, every some 5ul spore liquid.Record morbidity progression calculates disease and refers to and preventive effect behind the 4d.
It is following that disease refers to investigate grade scale:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Calculate preventive effect according to formula; Utilize the percentage and the probit value table of comparisons to convert preventive effect to probit value; Simultaneously dose concentration is converted into logarithm value, obtains independent formula Y=a+bX, calculate concentration EC in correlation coefficient r and the inhibition with dosage logarithm value (X) and probit value (y)
50
According to the synergistic effect that the method for Wadley is calculated mixed agent, SR is a coefficient of synergism, like SR＜0.5, and two kinds of medicaments antagonism that has been mixed then; 0.5≤SR≤1.5, then two kinds of medicaments summation action that has been mixed; SR＞1.5, then two kinds of medicaments synergistic effect that has been mixed.
Can find out that from table 1 polyoxin wants high to the specific activity bacillus subtilis of grey mould fruit rot of strawberry bacterium; Its composite back coefficient of synergism has synergistic effect preferably greater than 1.5.In addition, these two kinds of medicament mechanism of action are different, do not have mutual pesticide resistance problem, can be used for alleviating the resistance problem of polyoxin so these two types of medicaments are mixed, and prolong the service life of medicament.
The effect of table 1 polyoxin and bacillus subtilis and compound sterilizing combination control grey mould fruit rot of strawberry thereof
Embodiment 6
2.5% polyoxin, the 10000000000 bacillus subtilis wetting powders of getting embodiment 1 are as test medicine (group A); Contrast agents is respectively the 3% polyoxin wetting powder that Wuhan Nature provides (group B), and 20,000,000,000 spores/gram bacillus subtilis wetting powder (organizing C) of providing of Wuhan Kenuo Biology Science & Technology Co., Ltd..Test method adopts the bacteriostatic activity screening of exsomatizing, the potted plant medicament screening of live body respectively.
The bacteriostatic activity that exsomatizes screens: test medicine and medicament are diluted 1200 times; At the cucumber fusarium axysporum bacterium colony edge of cultivating 5d; Beat with the aseptic card punch of diameter 5mm and to get the bacterium cake, place and contain on the PDA flat board that dilutes medicament 1 in every ware; Every processing repeats for 3 times, is empty map with the medium that adds sterile water.Put into 25 ℃ of insulating boxs, cultivate 4d, measure colony diameter with the ruler interior extrapolation method.Calculate the germ growth inhibition ratio.
From table 2, can find out; Polyoxin and bacillus subtilis and compound sterilizing combination thereof all have better effects, particularly 2.5% polyoxin, 10,000,000,000 bacillus subtilises will exceed 10～20% to its each single agent of specific activity of watermelon blight to the stripped antibacterial of cucumber fusarium axysporum; In addition, these two kinds of medicament mechanism of action are different, do not have mutual pesticide resistance problem, can be used for alleviating the resistance problem of each single agent so these two types of medicaments are mixed, and prolong the service life of medicament.
Table 2 polyoxin and bacillus subtilis and compound sterilizing combination thereof are to the stripped fungistatic effect of cucumber fusarium axysporum
The potted plant medicament screening of live body (mixing local method): in the Hua Yang of Jurong City town peasant household test booth, carry out year October in September, 2008～2009.Activation cucumber fusarium axysporum in the PDA medium is cultivated 3d down for 25 ℃, changes the PD liquid nutrient medium again over to and cultivates 4～5d, and bacterium liquid filters, and removes mycelia, will filtrate through 5000rmin
-1Centrifugal 10min gets deposition and adds suitable distilled water diluting to become spore concentration be 1.0 * 10
5Individual ml
-1Suspension, subsequent use.Adopting the method for pudding treatment, with supplying reagent agent and nutrition soil native according to the medicine that mass concentration is configured to variable concentrations, is blank soil not mix medicament.Every kind of each concentration of medicament is a processing, and 3 repetitions are established in each processing, and each repeats to adorn 10 nutritive cubes.Select full cucumber seeds,, be seeded in sterilization 3 times, be covered with in the special-purpose cultivation of the insurance box of double gauze, in 25 ℃ of dark insulating boxs, cultivate vernalization with distilled water flushing through 0.1% mercuric chloride water logging kind 5min.When treating that the cucumber seeds radicle grows to 0.5cm, add 1.0 * 10
5Individual ml
-1Bacteria suspension in soak 30min, take out and to be sowed in the medicine soil.Treat to carry out after blank is fully fallen ill state of an illness classification investigation, and calculate disease index and control efficiency.
Cucumber fusarium axysporum severity grade scale:
0 grade, no illness;
1 grade, the cauline leaf light symptoms;
2 grades, plant is slightly wilted, and necrotic plaque appears in stem, the blade yellow;
3 grades, the plant moderate is wilted, the sagging yellow of blade;
4 grades, plant is seriously wilted, and it is withered to lodge.
From table 3, can find out; The combination of polyoxin and bacillus subtilis and compound sterilizing thereof is mixed local method and the control of cucumber fusarium axysporum is imitated body is antibacterial all to have certain effect, and especially each single agent will exceed 15～25% than it to the preventive effect of watermelon blight with 2.5% polyoxin, 10,000,000,000 bacillus subtilises; In addition, these two kinds of medicament mechanism of action are different, do not have mutual pesticide resistance problem, can be used for alleviating the resistance problem of each single agent so these two types of medicaments are mixed, and prolong the service life of medicament.
Table 3 is mixed polyoxin and bacillus subtilis under the local method and compound sterilizing combination thereof the effect to the cucumber fusarium axysporum control
Embodiment 7
In Zhenjiang research of agricultural science institute rice test field, carry out field control effectiveness test on August 15th, 2010 (paddy rice booting stage).The test water rice varieties is for pressing down rice No. 10.2.5% polyoxin, the 10000000000 bacillus subtilis wetting powders of getting embodiment 1 are as test medicine (group A); Contrast agents is respectively the 3% polyoxin wetting powder that Wuhan Nature provides (group B), and 20,000,000,000 spores/gram bacillus subtilis wetting powder (organizing C) of providing of Wuhan Kenuo Biology Science & Technology Co., Ltd..
Adopt spray-on process to prevent and treat rice sheath blight disease.Contain the phase dispenser at rice tillering, by every 667m
2Consumption is converted water 50Kg even spraying, every sub-district 133.4m
22 repetitions are established in each processing, separate with the little ridge between processing, and establish guard row.Investigate the banded sclerotial blight disease index of each sub-district behind the medicine in 15 days, calculate control efficiency.
State of an illness grade scale is following
0 grade: no scab;
1 grade: base portion has a small amount of scab;
2 grades: base portion has more scab, and rises to the 2nd leaf (4 leaves);
3 grades: 3 leaves that fall have scab;
4 grades: 2 leaves that fall have scab;
5 grades: sword-like leave has scab or complete stool withered.
Table 4 polyoxin and bacillus subtilis and compound sterilizing combination thereof prevent and treat the field effect of rice sheath blight disease
As shown in table 4; 2.5% polyoxin, 10,000,000,000 withered grass buds were embraced after the pavilion bacterium wetting powder mu consumption 60g dispenser 15 days; Control efficiency to rice sheath blight disease is 79.63%; Control efficiency is superior to the control efficiency of 3% polyoxin 100g and 20,000,000,000 each single agent of bacillus subtilis 60g, and exceeding respectively is 12.73% and 22.68%, and field control synergy is remarkable.
The above only is a preferred implementation of the present invention; Be noted that for those skilled in the art; Under the prerequisite that does not break away from the principle of the invention, can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (4)
1. a bactericidal composition is characterized in that, the active component of said composition comprises bacillus subtilis and polyoxin, and said active component accounts for 10 ~ 85% of gross weight, and said bacillus subtilis accounts for 10 ~ 70% of active component weight.
2. a bactericidal composition is in the application that prevents and treats grey mould fruit rot of strawberry.
3. a bactericidal composition is in the application that prevents and treats cucumber fusarium axysporum.
4. a bactericidal composition is being prevented and treated the application of rice sheath blight disease.
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| CN2011102343144A CN102356771A (en) | 2011-08-16 | 2011-08-16 | Sterilization composition and use thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102876604A (en) * | 2012-09-23 | 2013-01-16 | 江苏丘陵地区镇江农业科学研究所 | Bacillus subtilis DJ-6 and application thereof to prevention and treatment of strawberry disease |
| WO2013178653A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of amino acid or protein biosynthesis, inhibitors of atp production and inhibitors of the cell wall synthesis |
| CN104507316A (en) * | 2012-05-30 | 2015-04-08 | 拜尔农作物科学股份公司 | Composition comprising a biological control agent and a fungicide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013178653A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of amino acid or protein biosynthesis, inhibitors of atp production and inhibitors of the cell wall synthesis |
| CN104507316A (en) * | 2012-05-30 | 2015-04-08 | 拜尔农作物科学股份公司 | Composition comprising a biological control agent and a fungicide |
| CN104507317A (en) * | 2012-05-30 | 2015-04-08 | 拜尔农作物科学股份公司 | Composition comprising a biological control agent and a fungicide selected from inhibitors of amino acid or protein biosynthesis, inhibitors of ATP production and inhibitors of the cell wall synthesis |
| US9380787B2 (en) | 2012-05-30 | 2016-07-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of amino acid or protein biosynthesis, inhibitors of ATP production and inhibitors of the cell wall synthesis |
| CN104507316B (en) * | 2012-05-30 | 2018-03-06 | 拜尔农作物科学股份公司 | Composition comprising biocontrol agent and fungicide |
| CN104507317B (en) * | 2012-05-30 | 2019-11-15 | 拜尔农作物科学股份公司 | Composition comprising biocontrol agent and fungicide, and application thereof, kit |
| CN102876604A (en) * | 2012-09-23 | 2013-01-16 | 江苏丘陵地区镇江农业科学研究所 | Bacillus subtilis DJ-6 and application thereof to prevention and treatment of strawberry disease |
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Application publication date: 20120222 |