KR20140042268A - Hydroponic culture media for ginseng and preparation method thereof - Google Patents

Abstract

The present invention relates to a nutrient solution for hydroponically cultivating ginseng and a preparation method thereof. More specifically, as a nutrient solution for hydroponically cultivating ginseng to which herbal extracts and EM culture solutions are added and mixed in a low-concentration base nutrient solution for lettuce hydroponics, the present invention promotes a growth of ginseng in hydroponics and prevents damping-off, which is a leading horticultural disease acquired by the ginseng. In addition, organosynthetic pesticides are not used in a cultivation of the ginseng, thereby enabling a cultivation of environmentally-friendly ginseng. [Reference numerals] (AA) Base nutrient solution; (BB) Adding herbal extracts and EM culture solutions; (CC) Mixing; (DD) Nutrient solution for ginseng hydroponic cultivation

Description

Technical Field [0001] The present invention relates to a hydroponic culture medium for ginseng cultivation,

The present invention relates to a nutrient solution for cultivating ginseng and a method for manufacturing the same, and more specifically, a nutrient solution capable of promoting the growth of ginseng and increasing the antibacterial activity against pathogens of ginseng by adding an antimicrobial medicinal plant extract and an EM culture solution to the plant cultivation nutrient solution. And to a method for producing the same.

Ginseng (Panax ginseng C.A. Meyer) is a perennial herb that belongs to Araliaceae. It is known as a medicinal herb medicine which is very important in oriental medicine. It is included in Donguibogam in Korea and many ancient medicine books in China. Ginseng is used for treatment of weakness, boredom, fatigue, anorexia, vomiting and diarrhea. It helps the lung function, produces the secretion and enhances the anesthetic function and new function. The pharmacological effects of ginseng have been reported in cerebral cortex excitation and inhibition, equilibrium, anti-fatigue, anti-aging, immune enhancement, cardiac contraction, gonadal stimulation, hyperglycemia inhibition, protein synthesis promotion, homeostasis, anticancer and detoxification. The consumption of ginseng has been increasing rapidly due to the increase of consumers' need for health promotion and spreading to East as well as the world.

Ginseng is a perennial crop that requires 4 to 6 years of growing time to harvest. Since it is very low in land productivity compared to other crops and grows in one place during cultivation, it is more affected by soil characteristics than other crops. Many diseases such as diseases and plague are widespread. To prevent these diseases, chemical pesticides are often used. However, as consumer interest in health has increased recently, the consumption of environmentally friendly agricultural products has increased, and in the case of ginseng, cultivation of environmentally friendly farming methods has become necessary. Ginseng cultivated in hydroponics is not processed as clean agricultural products, but it is a tendency to use not only roots but also leaves and stalks in spices and vegetables.

Recently, the ginseng cultivation method using hydroponic culture method (Kim, Keum-Sook et al., 2009. The effect of cultivation environment on the ginsenoside content of hydroponically grown ginseng, The Korean Society of Medicinal Crop Science, 2009 Symposium and Spring Conference, , Journal of the Korean Society of Medicinal Crop Science, Vol.13, No.1, pp.157-169, 1981. The effects of temperature and growth time on the growth characteristics and yield of ginseng in hydroponically grown ginseng. , 20, 184). The patent on the hydroponics of ginseng is as follows. Korean Patent Registration No. 10-0959254 (Clean Ginseng and Ginseng Leaf Production Method) is used to prepare seedling ginseng which was stored at low temperature by using Baejigyeong ginseng hydroponic cultivation system and ginseng ginseng hydroponic cultivation system, After 5 months, the fresh ginseng and ginseng leaves are harvested. Korean Patent Registration No. 10-0960947 (Ginseng Hydroponic Cultivation Method) is a new ginseng hydroponic cultivation method which can obtain an excellent growth effect and an effect of increasing the saponin content without using pesticides. It is a step of sterilizing ginseng in an acidic solution, Or submerged in underground water to grow ginseng hydroponically.

When ginseng is cultivated hydroponically, the growth period is shorter than that of soil cultivation, there is no interruption period, productivity per unit area is increased, and disease is reduced. However, in hydroponic cultivation, transmission from seedling seeds, If infestation occurs due to infectious diseases, infectious diseases, infectious diseases, infectious diseases, infectious diseases, infectious diseases, infectious diseases, infectious diseases, infectious diseases, etc., it is necessary to pay close attention and care in hydroponic cultivation.

On the other hand, the control by the organic synthetic pesticides of the plant diseases is concerned with the natural plant phytochemicals having excellent activity against the phytopathogenic fungi due to the continuous use and abuse of the pesticides and the destruction of the ecosystem, It is getting higher. The antimicrobial effect of the plant extracts of the promiscuous plant, Venturia inaequalis, which is an apple black rot (Gilliver, K. 1947. The effect of plant extracts on the germination of the conidia of Venturia inaequalis. Ann. Appl. Biol., 34, Effect of Guar and Cyamopsis tetragonolobus Root Extract on the Root Disease of Wheat by Biopolaris sorokiniana (Johnson, DA and LE Clark. 1979. Effect of Guar and Guar Extracts on Common Root of Winter Wheat and Spore Germination of Biopolaris sorokiniana. (Valsa mali) (1988). Development of a plant-based fungicide for the control of apple-tree blight (1), Plant Protection, 30, 24 ), And the effect of the extract of Momordica japonica on the vegetative gray mold (Bortrytis cinerea) (Baek, Sung-bong, 1989. Search for and use of antagonistic plants for the control of vegetative gray mold disease in Korea, 17, 39) . In this study, we investigated the effects of antagonistic antibiotics on the control of antibiotics using antagonistic antibiotics. , 4, 305), Effects of Promicromonospora sp. On ginseng root rot caused by Fusarium solani (Kim Sung Il, Lee Min-woong, 1994. Root microbial and soil disease control - Effects of antibiotics on ginseng root hospital - 50), the effect of Bacillus subtilis on apple gray mold (Jung Il et al., 2000. Microbiological control of gray mold disease, Korean Society for Organic Agriculture, 8, 121), Bacillus subtilis, 2006. Selection of antagonistic microorganisms for major diseases of ginseng, Journal of Korean Medicinal Plants, 14, 202).

The inventors of the present invention have studied the nutrient solution for the hydroponic cultivation of ginseng and have developed a nutrient solution having the effect of controlling the pathogenic bacteria such as ginseng gall bladder and pest, while accelerating the growth of ginseng by adding the herbal medicine extract and the EM culture solution to the known nutrient solution Thereby completing the invention.

When the ginseng is cultivated by hydroponics, the growth period is shorter than that of the soil cultivation, there is no period of interruption, the productivity per unit area is increased and the disease is reduced. In the hydroponic cultivation, however, the propagation from seedling seeds, There is a drawback that it is liable to be widespread when infecting the germs by infection through ground water or spring water, or by infecting from a passenger. The present invention is to improve the above problems to promote the growth of ginseng and to provide a nutrient solution that can increase the antibacterial activity of the pathogen of ginseng.

The present invention relates to a ginseng hydroponic nutrient solution containing a herbal medicine extract and an EM culture medium as an active ingredient, which promotes the growth of ginseng in hydroponic culture and enhances the antimicrobial activity against germinous pathogens, thereby increasing the yield of ginseng And can produce clean ginseng of excellent quality.

It is possible to cultivate ginseng in a short period of time by cultivating ginseng in aerial cultivation or hydroponic cultivation by using a nutrient solution containing an extract of herbal medicine and EM as an active ingredient and to cultivate clean ginseng in a short period of time. And the productivity is increased, thereby improving the productivity.

FIG. 1 is a flow chart of a manufacturing process of a nutrient solution for ginseng hydroponics.

The present invention relates to a method for producing a nutrient solution for ginseng hydroponics (see Fig. 1), which comprises mixing a herbal medicine extract and an EM culture medium in a nutrient solution containing a nutrient solution for Yamazaki lettuce cultivation.

The basal nutrient solution may be adjusted to be S 1.0me / L and Fe EDTA 3.0, Mn 0.5, B 0.5, Zn 0.05, Mo 0.05, Cu 0.02, Co 0.01mg / L in Yamazaki lettuce cultivation nutrient solution.

In the above, the extract of the herb medicine may be any one or more extracts selected from the group consisting of a herbal extract, a white pine bark, a ginseng extract, a ginseng extract, a rhubarb, a yellow dragonfly, a bamboo shoot, a foxtail, a foliage horse, an evening primrose,

Herbal medicine extract may be prepared by adding 0.1 to 0.5% by weight based on the total weight of the basal nutrient solution, 0.1 to 0.2% by weight EM culture solution.

The present invention includes a nutrient solution for ginseng hydroponics produced by the above-mentioned method.

The contents of the present invention are described in more detail below.

The present invention includes a step of producing a herbal medicine extract and a step of mixing a herbal medicine extract and an EM culture liquid into a nutrient solution for Yamazaki lettuce cultivation.

1) Preparation of herbal medicine extract

The herbal medicines are re-dried each commercially available herbal medicines and pulverized to 20 mesh or less, and 2L of 95% ethanol per 100g of sample is extracted twice for 5 hours, mixed with primary and secondary extracts, and concentrated in vacuo to 95%. Prepare an ethanol extract. The 95% ethanol extract residue was put again 3L purified water and extracted for 5 hours and mixed with the 95% ethanol extract and concentrated in vacuo so that the solid content concentration is 10% to prepare a herbal extract.

2) Preparation of EM culture medium

The EM culture medium was prepared by a known method. That is, after 20 g of molasses was dissolved in 2 L of rice, 20 mL of EM stock solution (EVER MIRACLE CO., LTD.) Was mixed and allowed to stand at 25 ° C for 7 days to prepare an EM culture solution.

3) Manufacture of basic nutrient solution

Yamazaki nutrient solution for lettuce cultivation (N 6.0, P 1.5, K 4.0, Ca 2.0, Mg 1.0me / L) in S 1.0me / L and Fe EDTA 3.0, Mn 0.5, B 0.5, Zn 0.05, Mo 0.05, Cu 0.02, Basal nutrient solution was prepared so that Co 0.01 mg / L.

4) Manufacture of nutrient solution for ginseng hydroponics

The nutrient solution for ginseng hydroponics was prepared by adding 0.1 ~ 0.5% of herbal medicine extract to 0.1 ~ 0.2% of EM medium in the above nutrient solution.

Hereinafter, the present invention will be described in detail with reference to Examples and Test Examples. However, these are for the purpose of illustrating the present invention in more detail, and the scope of the present invention is not limited thereto.

≪ Example 1-6 >

The nutrient solution for ginseng hydroponics was prepared at the blending ratio shown in Table 1. Control 1 is the basal nutrient solution and Control 2 is the EM nutrient solution added to the basal nutrient solution.

In the following Table 1, the EM culture medium was prepared by dissolving 20 g of molasses in 2 L of rice flour, mixing 20 mL of EM stock solution (EVER MIRACLE CO., LTD.), And culturing at 25 ° C for 7 days.

In Table 1, the herbal extracts were ground to 20 mesh each herbal medicine, put 2L of 95% ethanol per 100g of herbal medicine samples and extracted twice for 5 hours to mix the primary and secondary extracts and then concentrated in vacuo to 95% ethanol extract. Was prepared. The 95% ethanol extraction residue was added to 3L of purified water and extracted for 5 hours, mixed with the 95% ethanol extract, and used by vacuum concentration to obtain a solid content of 10%.

In the Table 1, the basic nutrient solution is lettuce 1.0 hydroponic Yamazaki nutrient solution (N 6.0, P 1.5, K 4.0, Ca 2.0, Mg 1.0 me / L) S 1.0me / L and Fe EDTA 3.0, Mn 0.5, B 0.5, Zn 0.05 , Mo 0.05, Cu 0.02, Co was added to 0.01 mg / L and prepared to use.

Composition of nutrient solution for ginseng hydroponics (Unit:% by weight) division Control 1 Control 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Herbal medicine extract GoSam - - 0.1 - - 0.1 - 0.1 - Maple leaves - - - 0.1 - - 0.1 - 0.1 Ivy - - - - 0.1 - - 0.1 0.1 rhubarb - - 0.1 - - 0.1 - - - Mural - - - - 0.1 - 0.1 - 0.1 Ringworm - - - 0.1 - - 0.1 - 0.1 How - - - - - 0.1 - 0.1 - Kool - - - - - 0.1 - 0.1 - Indwelling - - - - 0.1 - 0.1 - - Bamboo shoot - - - 0.1 - 0.1 - - 0.1 goldthread - - - - 0.1 - 0.1 0.1 - EM medium - 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Basic nutrient solution 100 99.8 99.6 99.5 99.4 99.3 99.3 99.3 99.3 Sum 100 100 100 100 100 100 100 100 100

<Experimental Example 1> Measurement of growth condition of ginseng cultivated in hydroponic culture

The ginseng hydroponics were grown in a solid culture medium and the medium was mixed with 4: 6 ratio of Horticultural soil (Dongbu Palm Hanon) and Perlite 3 (Kyungdong Ceratech). The pH of the nutrient solution was adjusted to 6.2 and the concentration of EC to 1.0 mS / cm, and the feeding frequency of the nutrient solution was supplied three times at intervals of 10 days. The temperature was maintained at 22 ℃ and the relative humidity was 75%, and the light intensity was adjusted to 6000 ~ 10,000 lux.

The seedling ginseng was selected from fresh weight of 0.7 ~ 1.0g, and after four months, samples were collected to investigate the growth condition of ginseng. The root length was the length from the top of the head to the end of the ginseng leaf. The root length was the length from the root of the brain to the longest root, and the root was from the head Diameter of the 1 cm area. And the mass is the weight of the root below the head, and the live weight is the weight of the plant front after harvest. Table 2 shows the results of the investigation on the growth status of ginseng cultivated in hydroponics.

Growth Status of Hydroponically Cultivated Ginseng division Control 1 Control 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 (G) 0.91 0.90 0.88 0.86 0.85 0.89 0.86 0.84 0.83 Growth status after harvest


Extra long (cm) 13.2 14.1 17.2 17.9 17.6 18.2 17.4 18.1 17.7 Root (cm) 14.0 15.7 18.5 18.6 18.2 19.1 18.3 18.8 18.6 (G) 3.6 3.8 4.3 4.6 4.4 4.8 4.7 4.9 4.6 Live weight (g) 5.8 6.1 6.7 7.2 6.9 7.6 7.5 7.7 7.4

As shown in Table 2, the growth of ginseng was found to be better than that of control 1 and 2 in the addition of the herbal medicine extract and the EM culture medium to the nutrient solution during ginseng hydroponics.

&Lt; Experimental Example 2 > Measurement of morbidity rate of wearing bottles of hydroponic cultured ginseng

Rhizoctonia solani strain, which is a pathogenic ginseng root pathogenic bacterium isolated from ginseng, was inoculated on a potato dextrose agar and incubated at 25 ° C for 7 days in sterilized distilled water at a ratio of 20 times. The suspension was added to the nutrient solution at 0.3% And the morbidity rate of the ginseng was investigated for 30 days. Table 3 shows the prevalence of wearing disease of hydroponically cultured ginseng.

Purchase Disease Rate of Hydroponic Cultured Ginseng division Control 1 Control 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Disease rate (%) 78 32 2 One 0 One 0 0

As shown in the above Table 3, the ginseng supplied with the nutrient solution of the example was significantly lower than that of control 1 (78%) and 2 (32%), It is very good.

It is highly likely to be used industrially because it promotes the growth of ginseng in the hydroponic cultivation of ginseng and the prevention effect of the wearing disease frequently occurs frequently in hydroponic cultivation.

Claims (5)

A method for producing cultivated nutrient solution for ginseng hydroponic cultivation, comprising mixing the medicinal herb extract and EM culture solution in the basal nutrient solution containing the Yamazaki lettuce cultivation solution. The basal nutrient solution is adjusted to be S 1.0me / L and Fe EDTA 3.0, Mn 0.5, B 0.5, Zn 0.05, Mo 0.05, Cu 0.02, Co 0.01 mg / L in Yamazaki lettuce cultivation nutrient solution. Method of producing nutrient solution for ginseng hydroponic culture. The ginseng hydroponic nutrient solution according to claim 1, wherein the herbal medicine extract is one or more extracts selected from the group consisting of a herbal extract, a white ginseng, a white ginseng, a ginseng, a ginseng, a rhubarb, &Lt; / RTI &gt; The method of claim 1, wherein the herbal extract is added so that 0.1 to 0.5% by weight of the basal nutrient solution, EM culture solution is 0.1 to 0.2% by weight. A nutrient solution for ginseng hydroponics produced by the method of any one of claims 1 to 4.

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