CN112674115A - Compound preparation for preventing and treating ginger stem rot and application thereof - Google Patents
Compound preparation for preventing and treating ginger stem rot and application thereof Download PDFInfo
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- CN112674115A CN112674115A CN202110082374.2A CN202110082374A CN112674115A CN 112674115 A CN112674115 A CN 112674115A CN 202110082374 A CN202110082374 A CN 202110082374A CN 112674115 A CN112674115 A CN 112674115A
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Abstract
A compound preparation for preventing and treating stem rot of ginger comprises active ingredients including Bacillus subtilis active compound and amino-oligosaccharin active compound; the mass ratio of the number of live bacillus of bacillus subtilis to the raw amino-oligosaccharin is 20000 hundred million: 0.5g to 3.5 g; the content of active ingredients of the amino-oligosaccharide raw drug is 0.5 percent, and the content of bacillus subtilis raw drug is 100 hundred million/g. The preparation of the compound preparation comprises the steps of mixing raw materials, mechanically crushing and superfine crushing. The compound preparation has obvious effect of preventing and treating the ginger stem basal rot, and the effect is improved by about 30 percent compared with the effect of single dose of amino-oligosaccharin and bacillus subtilis; after the compound preparation for preventing and treating the ginger stem basal rot is used, the average growth diameter of bacterial colonies can be reduced to 1.57cm, and the inhibition rate of the ginger stem basal rot can reach 87.41%.
Description
Technical Field
The invention relates to the technical field of ginger bactericidal preparations, in particular to a compound preparation for preventing and treating ginger stem rot and application thereof.
Background
The basal rot of the ginger is also called as pythium root rot, after the ginger is diseased, the basal part of the stem begins to generate water stain-shaped light brown disease spots, the leaves turn yellow from bottom to top, the basal part of the stem inclines after being soft, and the plant withers and dies after a while. If the disease condition is slightly mild, the yield can be reduced by 10% -30%, and if the disease condition is serious, the yield can be reduced by more than 50%. The most commonly used methods at present are: treating ginger seeds, selecting ginger seeds, and avoiding using ginger blocks of the last year disease-causing land as ginger seeds; strengthening cultivation management; an elevated sunshade net is erected to build microclimate and reduce the incidence of diseases; soil treatment and chemical control. The pesticide effect prevention and treatment is the most common method, but the existing pesticide effect prevention and treatment method has the defects of high use cost, difficulty in large-area popularization and use, unobvious use effect and the like.
The patent CN107212021A discloses a microbial agent for preventing and treating ginger stem basal rot, a preparation method and application thereof, wherein the microbial agent comprises a trichoderma longibrachiatum fermentation product and a mixed material, and the mixed material is humic acid, amino acid and medical stone; trichoderma longibrachiatum fermentation product: humic acid: amino acids: the medical stone comprises the following components in percentage by mass: (3-6): (1-2): 4-5), the effective viable count in the trichoderma longibrachiatum fermentation product is more than or equal to 2 x 109/g. However, the effect of the microbial agent is not remarkable.
Patent CN101999371A discloses a preparation for preventing and treating ginger stem basal rot, which comprises the following components by weight: 54-58% of 25% metalaxyl, 5.5-6.5% of 95% hymexazol and 36-40% of 20% copper succinate + glutarate, but the preparation has high use cost and is difficult to popularize and use in a large area.
Patent CN105660704A discloses a bacillus subtilis and amino-oligosaccharin compound composition, a compound preparation and application thereof, wherein the bacillus subtilis and amino-oligosaccharin compound composition comprises bacillus subtilis and amino-oligosaccharin; the mass ratio of the number of live bacillus of bacillus subtilis to the number of amino-oligosaccharins is 20000 hundred million: 1.5g to 3.5 g. However, the product of the patent can only be used for the watermelon fusarium wilt, and the application range is narrow.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides the compound preparation for preventing and treating the ginger stem basal rot, which can reduce the cost of the prior art for preventing and treating the ginger stem basal rot, improve the inhibition rate of bacterial colonies and carry out large-area use.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a compound preparation for preventing and treating stem rot of ginger comprises active ingredients including Bacillus subtilis active compound and amino-oligosaccharin active compound; the mass ratio of the number of live bacillus of bacillus subtilis to the raw amino-oligosaccharin is 20000 hundred million: 0.5 to 3.5 g.
The content of active ingredients of the amino-oligosaccharide raw drug is 0.5 percent, and the content of bacillus subtilis raw drug is 100 hundred million/g.
The invention also provides a preparation method of the compound preparation for preventing and treating the stem base rot of the ginger, which comprises the steps of mixing raw materials, mechanically crushing and ultramicro crushing;
mixing the raw materials, respectively weighing an amino-oligosaccharide raw drug, a bacillus subtilis raw drug, a dispersing agent, a wetting agent, a disintegrating agent, a pH regulator, a stabilizing agent and a filler according to a ratio, and uniformly mixing to obtain a raw material mixture;
the raw material mixture contains 0.1-10 wt% of amino-oligosaccharin, 5-30 wt% of dispersing agent, 5-10 wt% of wetting agent, 1-30 wt% of disintegrating agent, 1-2 wt% of pH regulator, 1-3 wt% of stabilizer and the balance of filler.
Preferably, the raw amino-oligosaccharin in the raw material mixture is 0.5 wt%, and the mass ratio of the number of live bacillus subtilis to the number of amino-oligosaccharin is 20000 hundred million: 0.5g, 10 percent of dispersing agent, 6 percent of wetting agent, 20 percent of disintegrating agent, 1.5 percent of PH regulator, 2 percent of stabilizer and the balance of filling material.
The dispersant is one or more of sodium lignosulfonate, polycarboxylate, naphthalene sulfonate, naphthalene sulfonic acid formaldehyde condensate sodium salt and the like.
The wetting agent is one or more of polyacrylate, isobutylene/maleic acid ester, starch/polyacrylate, etc.
The disintegrating agent is one or more of polyvinyl alcohol/polyacrylate, acrylic fiber hydrate, and polymeric amino acid.
The pH regulator is one or more of triethanolamine, citric acid, etc.
The stabilizer is one or more of 2, 6-di-tert-butyl-4-methylphenol, zinc citrate and the like.
The filler is one or more of clay, pottery clay, diatomite, talcum powder, kaolin, corn starch, glucose, white carbon black, etc.
And mechanically crushing the mixture to obtain a coarse powder mixture, wherein the crushing fineness of the coarse powder mixture is as follows: the amount of the test sieve passing through the 45um is larger than or equal to 95 percent;
and (2) performing superfine grinding, namely slowly adding the coarse powder mixture into a superfine airflow grinder with a freeze dryer for superfine grinding to obtain the compound bactericide containing the amino-oligosaccharin and the bacillus subtilis, wherein: the crushing fineness of the compound bactericide of the amino-oligosaccharin and the bacillus subtilis is as follows: the amount of the test sieve passing through the 25 mu m is larger than or equal to 95 percent.
Compared with the prior art, the invention has the beneficial effects that:
(1) the compound preparation for preventing and treating the ginger stem basal rot has obvious effect of preventing and treating the ginger stem basal rot, and the effect is improved by about 30 percent compared with that of single dose of amino-oligosaccharin and bacillus subtilis;
(2) after the compound preparation for preventing and treating the ginger stem basal rot is used, the average growth diameter of bacterial colonies can be reduced to 1.57cm, and the inhibition rate of the ginger stem basal rot can reach 87.41%;
(3) in the compound preparation for preventing and treating the stem root rot of the ginger, the application concentrations of the amino-oligosaccharin and the bacillus subtilis are in positive correlation with the inhibition rate, the application EC50 of a single amino-oligosaccharin dose is 2458.61mg/L, the application EC50 of a single bacillus subtilis dose is 1256.38mg/L, and the application EC50 of the compound amino-oligosaccharin of the bacillus subtilis is 458.77 mg/L.
Detailed Description
In order to more clearly understand the technical features, objects, and effects of the present invention, specific embodiments of the present invention will now be described.
Compounded formulation example 1
Mixing an amino-oligosaccharin raw material drug and a bacillus subtilis raw material drug to prepare a compound preparation, and diluting the total effective content in the compound preparation to 1000 ppm.
The content of active ingredients of the amino-oligosaccharide raw drug is 0.5 percent, and the content of bacillus subtilis raw drug is 100 hundred million/g.
The preparation method of the compound preparation comprises the steps of mixing raw materials, mechanically crushing and superfine crushing;
mixing the raw materials, respectively weighing an amino-oligosaccharide raw drug, a bacillus subtilis raw drug, a dispersing agent, a wetting agent, a disintegrating agent, a pH regulator, a stabilizing agent and a filler according to a ratio, and uniformly mixing to obtain a raw material mixture;
the raw material mixture contains 0.5 percent of amino-oligosaccharin raw material by weight, and the mass ratio of the number of live bacillus subtilis to amino-oligosaccharin is 20000 hundred million: 0.5g, 10 percent of dispersing agent, 6 percent of wetting agent, 20 percent of disintegrating agent, 1.5 percent of PH regulator, 2 percent of stabilizer and the balance of filling material.
The dispersing agent is sodium lignosulfonate, the wetting agent is starch/polyacrylate (starch grafted polyacrylate copolymer), the disintegrating agent is polyvinyl alcohol/polyacrylate (polyvinyl alcohol grafted polyacrylate polymer), the pH regulator is citric acid, the stabilizing agent is 2, 6-di-tert-butyl-4-methylphenol, and the filling material is a composition of diatomite, kaolin, corn starch and glucose.
And mechanically crushing the mixture to obtain a coarse powder mixture, wherein the crushing fineness of the coarse powder mixture is as follows: the amount of the test sieve passing through the 45um is larger than or equal to 95 percent;
and (2) performing superfine grinding, namely slowly adding the coarse powder mixture into a superfine airflow grinder with a freeze dryer for superfine grinding to obtain the compound bactericide containing the amino-oligosaccharin and the bacillus subtilis, wherein: the crushing fineness of the compound bactericide of the amino-oligosaccharin and the bacillus subtilis is as follows: the amount of the test sieve passing through the 25 mu m is larger than or equal to 95 percent.
Compounded formulation example 2
Mixing amino-oligosaccharin raw material and bacillus subtilis raw material to prepare a compound preparation, and diluting the total effective content in the compound preparation to 2000 ppm.
The content of the active ingredients of the amino-oligosaccharide technical material and the content of the bacillus subtilis technical material are the same as the compound preparation in example 1.
The preparation method and the raw material composition of the compound preparation are the same as those of the compound preparation in example 1.
Compounded formulation example 3
Mixing amino-oligosaccharin raw material and bacillus subtilis raw material to prepare a compound preparation, and diluting the total effective content in the compound preparation to 3000 ppm.
The content of the active ingredients of the amino-oligosaccharide technical material and the content of the bacillus subtilis technical material are the same as the compound preparation in example 1.
The preparation method and the raw material composition of the compound preparation are the same as those of the compound preparation in example 1.
Comparative example 4
Comparative runs 1-6 were set up for comparison.
Comparative example 1: a comparison test is carried out by adopting the bacillus subtilis with the dilution concentration of 1000ppm, and the bacterial content of the original bacillus subtilis is 100 hundred million/g before dilution.
Comparative example 2: a comparative test is carried out by adopting the bacillus subtilis with the dilution concentration of 2000ppm, and the bacterial content of the original bacillus subtilis is 100 hundred million/g before dilution.
Comparative example 3: a comparative test is carried out by adopting the bacillus subtilis with the dilution concentration of 3000ppm, and the bacterial content of the original bacillus subtilis is 100 hundred million/g before dilution.
Comparative example 4: a comparison test is carried out by adopting the amino-oligosaccharin with the dilution concentration of 1000ppm, and the effective content of the amino-oligosaccharin raw medicine before dilution is 0.5%.
Comparative example 5: a comparative test is carried out by adopting the amino-oligosaccharin with the dilution concentration of 2000ppm, and the effective content of the amino-oligosaccharin raw medicine before dilution is 0.5%.
Comparative example 6: a comparison test is carried out by adopting the amino-oligosaccharin with the dilution concentration of 3000ppm, and the effective content of the amino-oligosaccharin raw medicine before dilution is 0.5%.
Comparative example 7: a blank control was set.
Effect example 5
The drug effect test of the ginger stem basic rot pathogen according to the industry standard NY/T1156.6-2006 of Ministry of agriculture is as follows:
(1) the test conditions are as follows: the test is carried out in an intelligent greenhouse, the temperature is 25 +/-0.5 ℃, the illumination is 16h, the darkness is 8h, and the humidity is 60%.
(2) The number of test repetitions: each treatment was set to 3 replicates.
(3) The instrument equipment comprises: electronic balance (induction 0.1 mg), biological incubator, culture dish, graduated cylinder, quantifying bottle, pipette, beaker, inoculator, hole puncher.
(4) Preparing a test material: the ginger stem rot is cultured on a PDA culture medium for later use in the test.
(5) Medicament treatment: mixing with appropriate amount of different concentrations of medicinal agents with 50mLPDA culture medium by suspension method, pouring into 3 sterile culture dishes, solidifying, and inoculating rhizoma Zingiberis recens stem rot.
The suspension method comprises the following steps: preparing mother liquor of each test medicament by using sterile water on a sterile operating platform, and then diluting the mother liquor by using sterile water to prepare 3 liquid medicines with test concentration gradients. Mixing 1ml of the medicinal liquid with 49ml of PDA culture medium (melting temperature of PDA culture medium is about 50 deg.C after sterilization) with a pipette to obtain a series of medicated culture medium, adding 1ml of sterile water with equal volume as blank control, and cooling and solidifying the culture medium. Each treatment was set to 3 replicates.
(6) Inoculation and culture: cutting off fungus cakes from the edges of bacterial colonies by using a sterilization puncher with the diameter of 0.5cm under aseptic conditions, inoculating the fungus cakes to the center of a drug-containing flat plate by using an inoculator, covering a dish cover, culturing for a certain time at 25 ℃, measuring the diameter of the bacterial colonies, and calculating the inhibition percentage.
(7) The investigation method comprises the following steps: after the pathogenic tissue is inoculated, the culture is carried out for a certain time, the colony diameter is measured by a cross rule, the average value is calculated, and the relative inhibition rate is calculated. And (4) converting the data, and performing regression analysis to obtain a virulence regression equation and an EC50 value.
The calculation formula is as follows:
(7) and (4) analyzing results: the results of the measurement of the inhibition rate of the amino-oligosaccharin and the bacillus subtilis culture medium on the ginger stem-based saprophytic hyphae are shown in table 1.
Wherein x in the regression equation is the logarithm of the dose, and y is the probability value of the inhibition rate. When the mass ratio of the bacillus subtilis to the amino-oligosaccharin is constant, the inhibition rate, the regression equation, the correlation coefficient R and the EC50 are not influenced. The correlation coefficient R represents a linear correlation relationship between two variables, and when R is greater than 0, the two variables are in positive correlation; when R is less than 0, the two variables are in negative correlation. The absolute value of R is between 1 and-1. The closer the absolute value of R is to 1, the stronger the linear correlation of the two variables is; the absolute value of R is close to 0, indicating that there is almost no linear correlation between the two variables. Generally, when the absolute value of R is greater than 0.75, the two variables are considered to have a strong linear correlation. EC50 refers to the dose or effective median concentration required to inhibit 50% of germ spore germination. The correlation coefficient R and EC50 are obtained by DPS software, professional statistics, biological determination and counting type data machine value analysis.
Statistical analysis is carried out on the data in the table 1, under the condition of a 0-concentration culture medium of a sample, the average growth diameter of 10d colonies can reach 9cm, and the increase of the colony diameter is obviously reduced along with the increase of the contents of the amino-oligosaccharin and the bacillus subtilis sample in the culture medium. The average growth diameters of bacterial colonies in the culture medium are respectively reduced to 3.3 cm and 1.57cm when the bacillus subtilis compound preparation is at 1000mg/L and 3000 mg/L. The inhibition rate of the bacillus subtilis compound preparation sample on the rhizoctonia solani and the treatment concentration of the medicament have better correlation.
Therefore, the effect of preventing and treating the ginger stem basal rot by compounding the bacillus subtilis and the amino-oligosaccharin is obviously improved by about 30 percent compared with the effect of single dose of the amino-oligosaccharin and the bacillus subtilis.
All percentages used in the present invention are mass percentages unless otherwise indicated.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A compound preparation for preventing and treating stem rot of ginger is characterized in that the effective components comprise a bacillus subtilis original drug and an amino-oligosaccharin original drug; the mass ratio of the number of live bacillus of bacillus subtilis to the raw amino-oligosaccharin is 20000 hundred million: 0.5-3.5 g; the content of active ingredients of the amino-oligosaccharide raw drug is 0.5 percent, and the content of bacillus subtilis raw drug is 100 hundred million/g.
2. A preparation method of a compound preparation for preventing and treating ginger stem rot is characterized by comprising the steps of mixing raw materials, mechanically crushing and ultramicro crushing.
3. The preparation method of the compound preparation for preventing and treating stem rot of ginger according to claim 2, characterized in that the raw materials are mixed, and the raw amino-oligosaccharide raw material, the raw bacillus subtilis material, the dispersing agent, the wetting agent, the disintegrating agent, the pH regulator, the stabilizer and the filler are respectively weighed according to the mixture ratio and uniformly mixed to obtain a raw material mixture.
4. The preparation method of the compound preparation for preventing and treating ginger stem basal rot according to claim 3, characterized in that the raw material mixture contains 0.1-10 wt% of amino-oligosaccharide, 5-30 wt% of a dispersing agent, 5-10 wt% of a wetting agent, 1-30 wt% of a disintegrating agent, 1-2 wt% of a pH regulator, 1-3 wt% of a stabilizer, and the balance of a filler.
5. The preparation method of the compound preparation for preventing and treating ginger stem-base rot according to claim 4, characterized in that the raw material mixture contains 0.5% by weight of raw amino-oligosaccharide, 10% by weight of a dispersing agent, 6% by weight of a wetting agent, 20% by weight of a disintegrating agent, 1.5% by weight of a pH regulator, 2% by weight of a stabilizer and the balance of a filler.
6. The preparation method of the compound preparation for preventing and treating ginger stem root rot according to claim 5, characterized in that the used dispersing agent is one or more of sodium lignosulfonate, polycarboxylate, naphthalenesulfonate, sodium naphthalene sulfonate formaldehyde condensate and the like; the wetting agent is one or more of polyacrylate, isobutylene/maleic acid ester, starch/polyacrylate, etc.
7. The preparation method of the compound preparation for preventing and treating ginger stem base rot according to claim 5, characterized in that the disintegrating agent is one or more of polyvinyl alcohol/polyacrylate, acrylic fiber hydrate, poly-amino acid and the like; the pH regulator is one or more of triethanolamine, citric acid, etc.
8. The preparation method of the compound preparation for preventing and treating the stem rot of the ginger as claimed in claim 5, characterized in that the stabilizer is one or more of 2, 6-di-tert-butyl-4-methylphenol, zinc citrate and the like; the filler is one or more of clay, pottery clay, diatomite, talcum powder, kaolin, corn starch, glucose, white carbon black, etc.
9. The preparation method of the compound preparation for preventing and treating ginger stem base rot according to claim 2, characterized in that the mechanical crushing is performed, the mixture is mechanically crushed, and a coarse powder mixture is prepared, wherein the crushing fineness of the coarse powder mixture is as follows: the amount of the test sieve passing through the 45um is not less than 95 percent.
10. The preparation method of the compound preparation for preventing and treating ginger stem basal rot according to claim 2, characterized in that the coarse powder mixture is slowly added into an ultramicro jet mill with a freeze dryer for ultramicro grinding to obtain the compound bactericide containing the amino-oligosaccharide and the bacillus subtilis, wherein: the crushing fineness of the compound bactericide of the amino-oligosaccharin and the bacillus subtilis is as follows: the amount of the test sieve passing through the 25 mu m is larger than or equal to 95 percent.
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| CN202110082374.2A CN112674115A (en) | 2021-01-21 | 2021-01-21 | Compound preparation for preventing and treating ginger stem rot and application thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103300069A (en) * | 2013-05-22 | 2013-09-18 | 江苏省绿盾植保农药实验有限公司 | Biological bactericide composition and application thereof to wilt |
| CN105432667A (en) * | 2015-12-19 | 2016-03-30 | 佛山市艳晖生物科技有限公司 | Preparation method of bacillus subtilis wettable powder |
| CN105660704A (en) * | 2016-01-07 | 2016-06-15 | 海南利蒙特生物农药有限公司 | Bacillus subtilis and oligosaccharins compound composition, compound preparation and applications of compound composition and compound preparation |
| CN106135291A (en) * | 2016-07-14 | 2016-11-23 | 解晓燕 | A kind of prevent and treat soil-borne disease, improve crop cold resistance compositions |
-
2021
- 2021-01-21 CN CN202110082374.2A patent/CN112674115A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103300069A (en) * | 2013-05-22 | 2013-09-18 | 江苏省绿盾植保农药实验有限公司 | Biological bactericide composition and application thereof to wilt |
| CN105432667A (en) * | 2015-12-19 | 2016-03-30 | 佛山市艳晖生物科技有限公司 | Preparation method of bacillus subtilis wettable powder |
| CN105660704A (en) * | 2016-01-07 | 2016-06-15 | 海南利蒙特生物农药有限公司 | Bacillus subtilis and oligosaccharins compound composition, compound preparation and applications of compound composition and compound preparation |
| CN106135291A (en) * | 2016-07-14 | 2016-11-23 | 解晓燕 | A kind of prevent and treat soil-borne disease, improve crop cold resistance compositions |
Non-Patent Citations (2)
| Title |
|---|
| 孙广宇等: "《植物病理学实验技术》", 31 August 2002, 中国农业出版社 * |
| 马安民等: "《园林植物杀菌剂应用技术》", 31 October 2016, 河南科学技术出版社 * |
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Application publication date: 20210420 |