CN108041077A - A kind of soil fungicides - Google Patents

A kind of soil fungicides Download PDF

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Publication number
CN108041077A
CN108041077A CN201810042898.7A CN201810042898A CN108041077A CN 108041077 A CN108041077 A CN 108041077A CN 201810042898 A CN201810042898 A CN 201810042898A CN 108041077 A CN108041077 A CN 108041077A
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CN
China
Prior art keywords
bacillus subtilis
probenazole
humic acid
soil fungicides
soil
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CN201810042898.7A
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Chinese (zh)
Inventor
程亚南
张影
刘永卓
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Henan Institute of Science and Technology
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Henan Institute of Science and Technology
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Priority to CN201810042898.7A priority Critical patent/CN108041077A/en
Publication of CN108041077A publication Critical patent/CN108041077A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a kind of soil fungicides, active ingredient by：Probenazole, bacillus subtilis, humic acid composition, by 1,000,000,000,000 gemma of content/gram bacillus subtilis active compound be 100% active compound, probenazole, humic acid and the weight ratio of bacillus subtilis are 20 50:20‑50：1.3 active ingredient mechanism of action of the present invention are different each other, mutually mixed synergistic apparent, advantageously reduce applications of pesticide amount, reduce pesticide cost.

Description

A kind of soil fungicides
Technical field
The present invention relates to cultural control technical fields, are specifically a kind of soil fungicides, and the soil for preventing agriculturally passes Disease.
Background technology
Soil-borne disease refers to that pathogen such as fungi, bacterium, nematode and virus are lived in the soil with invalid body, and condition is suitable When from crop root or stem infringement crop caused by disease.The continuous cropping in soil, the raising of multiple crop index, and fertilising do not conform to Reason causes soil-borne disease increasing reason in recent years.Samping off, root rot, damping-off are the Major Diseases of crops seedling stage, It is general so that a small number of death of seedling, cause to lack row and break and ridge or even seedling is caused to lodge in flakes dead serious consequence.
The pathogen of soil-borne disease is just already present in soil before crop-planting, and people form medication not yet at present The custom of prevention is usually just to be watered spraying prevention using medicament after disease occurs, but is watered and sprays cumbersome, and drug effect It is bad, since disease cannot be prevented timely, cause the massive losses of part death of seedling.Therefore it is badly in need of a kind of soil disease Fungicide can in time be applied in crop sowing time, transplanting time, disease early period of origination, be the main way for solving above-mentioned technical problem Footpath.
The content of the invention
It is an object of the present invention to be directed to more than technical problem, a kind of soil fungicides are provided, it can effectively preventing soil biography Disease.
The technical solution used in the present invention is：A kind of soil fungicides, including active ingredient and adjuvant, wherein activity Ingredient is made of probenazole, humic acid and bacillus subtilis.
(1) probenazole, English name：Probenazole is the one of Japanese Meiji Seika Kaisha, Ltd.'s exploitation Kind of inducing immunity fungicide by the way that plant is excited to realize protection effect to immune (resistance) reaction of disease in itself, passes through Plant root absorbs, and relatively rapidly infiltration is conducted to plant each several part.
(2) bacillus subtilis, English name：Bacillus subtilis are a kind of microbial bactericides, by having The antibacterial material and site competition, the mode of action of induction of resistance of ingredient producing bacillus subtilis life are imitated, kills and control cause of disease Bacterium, so as to achieve the purpose that controlling disease.
(3) humic acid is a kind of natural organic high molecular compound, complicated, has ion exchange, chelating, wadding The functions such as solidifying and colloidal sol agriculturally play an important role of to improve soil, stimulating growth, are enhancing degeneration-resistant, improving quality.
The soil fungicides, by 1,000,000,000,000 gemma of content/gram bacillus subtilis active compound be 100% active compound, Probenazole, humic acid and the weight ratio of bacillus subtilis are 20-50:20-50：1, it is preferred that probenazole, rotten plant The weight ratio of acid and bacillus subtilis is 20:50:1 or 30:50:1.
The soil fungicides, the weight percent of probenazole are 2.0%-5.0%, the weight percent of humic acid Than for 2.0%-5.0%, the weight percent of bacillus subtilis is 0.05-0.1%, i.e., bacillus subtilis is in fungicide Content for 500,000,000 spores/gram~1,000,000,000 spores/gram.
The soil fungicides according to method known in those skilled in the art, add in corresponding auxiliary element, system It is standby into agriculturally acceptable granule.
The auxiliary element include filler, dispersant, wetting agent, binding agent, stabilizer or it is other be beneficial to effectively into Divide the substance stablized played with drug effect in the formulation, be all to be commonly used in pesticidal preparations or allow the various composition used.
The present invention has the following advantages and significant technique effect：
(1) mechanism of action of 3 active ingredients of the invention is different, and mutual mixture is synergistic apparent, can significantly carry High-drug-effect reduces Pesticide use amount and drug cost；Be conducive to that existing germ is delayed to prolong the potential resistance risk of single medicament The service life of long medicament.
(2) soil fungicides of the invention, are prepared into granule, easy to use, without being watered, time and labour saving.
Specific embodiment
Technical scheme and technique effect are described further below by way of specific embodiment, institute of the present invention The percentage stated is weight percentage.
First, indoor joint toxicity measuring test
Embodiment 1：Probenazole, bacillus subtilis, humic acid and its mixture are to the indoor virulence of rice seedling blight Measure (1) subjects：Rice seedling blight (Rhizoctonia solani), is gathered by field, is isolated and purified through interior.
(2) test method：After prerun determines the effective inhibition concentration scope of each medicament, by probenazole, withered grass gemma Bacillus, humic acid and its mixed agent dilute 5 concentration by active constituent content gradient series, are compared with sterile water.Reference《In Magnificent people's republic's agricultural industry criteria NY/T1156.2-2006》It carries out, is measured using mycelial growth rate method.It will be different dense Spend medicament to add in sterilizing culture dish, per ware 1mL, add the PDA culture medium 10mL of sterilizing fusing, be sufficiently mixed be made it is flat The bacterium colony to spread cultivation then is broken into the=bacterium dish of 0.8cm, per 1, ware, often handles 4 repetitions, be placed in 25 DEG C of insulating boxs and train by plate It supports, per colony diameter is measured 1 time with crossing method for 24 hours, calculates each processing net growth, mycelial growth inhibition rate.
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) is converted into numerical value (x), with minimum Square law acquires virulence regression equation (y=a+bx), and thus calculates the EC of each medicament50Value calculates medicine according to the abundant methods of Sun Yun The toxicity index and co-toxicity coefficient (CTC) of agent, evaluate the synergistic effect of pharmacy mix, i.e., as co-toxicity coefficient (CTC) >=120, Composition shows as synergistic effect；As CTC≤80, composition shows as antagonism；As 80 ＜ CTC ＜ 120, composition Show as summation action.
Result of the test is shown in Table 1.
1 probenazole of table, bacillus subtilis, humic acid and its mixture count the toxicity test of rice seedling blight Table
Indoor joint toxicity measuring the result shows that, when the weight ratio of probenazole, humic acid, bacillus subtilis is 20-50:20-50：When 1, co-toxicity coefficient (CTC) is all higher than 120, has apparent synergistic effect；When probenazole, rotten plant Acid, the weight ratio of bacillus subtilis are 20:50:When 1, co-toxicity coefficient (CTC) coefficient is maximum, and synergy is the most notable, in field There is good application value in experiment.
Embodiment 2：Probenazole, bacillus subtilis, humic acid and its mixture are to the indoor virulence of watermelon blight It measures
(1) subjects：Watermelon blight (Fusarium oxysporum f.sp.niveum.), is gathered by field, warp Interior isolates and purifies.
(2) test method：After prerun determines the effective inhibition concentration scope of each medicament, by probenazole, withered grass gemma Bacillus, humic acid and its mixture dilute 5 concentration by active constituent content gradient series, are compared with sterile water.Reference《China People's republic agricultural industry criteria NY/T1156.2-2006》It carries out, is measured using mycelial growth rate method.In aseptic condition Under, the liquid configured 5mL is taken to be uniformly mixed with 75mL culture mediums, 4 drug containing tablets are made, with equivalent sterile water and culture medium Mixing is as control.Cultured watermelon blight is aseptically cut with the sterilization punchers of diameter 5mm from edge Bacteria cake.With inoculator by pure culture biscuits involvng inoculation in drug containing tablet center, mycelia is face-down, is placed in 25 DEG C of incubators.Depending on blank control Growing state investigation result, each bacterium colony respectively once, take its average value with crossing method vertical measurement diameter.Calculate mycelia life Toxicity regression formula is obtained in long inhibiting rate, calculates EC50。
The synergistic effect of pharmacy mix is evaluated with the co-toxicity coefficient method (CTC) of the abundant methods of Sun Yun, i.e., CTC≤80 are that antagonism is made With 80<CTC<120 be summation action, and CTC >=120 are synergistic effect.
Toxicity test the results are shown in Table 2.
2 probenazole of table, bacillus subtilis, humic acid and its mixture are to the Toxicity Determination of watermelon blight Table
Table 2 the result shows that, mixed agent prevention watermelon blight, when probenazole, humic acid, bacillus subtilis weight Amount is than being 20-50:20-50：When 1, co-toxicity coefficient (CTC) is all higher than 120, has apparent synergistic effect；When probenazole, Humic acid, the weight ratio of bacillus subtilis are 20:50:When 1, co-toxicity coefficient (CTC) coefficient is maximum, has in field trials very Good application value.
2nd, embodiment is prepared
Embodiment 1：2.0% probenazole, 5.0% humic acid, 1,000,000,000 cfu/ grams of bacillus subtilis granule
Formula：According to probenazole, humic acid and bacillus subtilis and weight ratio be 20：50:1, it weighs according to quantity Probenazole 2.0%, humic acid 5.0%, bacillus subtilis 0.1%, sodium lignin sulfonate 3.5% (dispersant), 12 Sodium alkyl sulfate 2.5% (wetting agent), polyvinyl alcohol 4.2% (binding agent), white carbon 10% (filler), diatomite (filler) are mended Enough to 100%.
Embodiment 2：3.0% probenazole, 5.0% humic acid, 1,000,000,000 cfu/ grams of bacillus subtilis granule
Formula：It is 30 according to probenazole, humic acid and the weight ratio of bacillus subtilis：50:1, alkene is weighed according to quantity Propyl benzene thiazole 3.0%, humic acid 5.0%, bacillus subtilis 0.1%, naphthalenesulfonate formaldehyde condensation compound 2.5% (dispersant), Lauryl sodium sulfate 2.5% (wetting agent), polyvinyl alcohol 5.0% (binding agent), white carbon 15% (filler), bentonite (is filled out Material) complement to 100%.
Embodiment 3：2.0% probenazole, 2.0% humic acid, 5.0 hundred million cfu/ grams of bacillus subtilis granule
Formula：It is 20 according to probenazole, humic acid and the weight ratio of bacillus subtilis：20:When 1, weigh according to quantity Probenazole 2.0%, humic acid 2.0%, bacillus subtilis 0.05%, carboxymethyl cellulose 3.5% (dispersant), 12 Sodium alkyl sulfate 2.0% (wetting agent), polyvinyl alcohol 3.5% (binding agent), kaolin (filler) complement to 100%.
Embodiment 4：5.0% probenazole, 3.0% humic acid, 10.0 hundred million cfu/ grams of bacillus subtilis granule
Formula:It is 50 according to probenazole, humic acid and the weight ratio of bacillus subtilis：30:When 1.It weighs according to quantity Probenazole 5.0%, humic acid 3.0%, bacillus subtilis 0.1%, carboxymethyl cellulose 5% (dispersant), dodecane Base benzene sulfonic acid sodium salt 3.5% (wetting agent), polyvinyl alcohol 4.2% (binding agent), white carbon (filler) 10%, kaolin (filler) are mended Enough to 100%.
Prepare the preparation method of embodiment 1-4：Each component is sufficiently mixed by formula rate, crush, add water soak after again It stirs, then is granulated with screw extruder pelletizer, sieved after dry to get withered containing probenazole humic acid The fungicide product of careless bacillus, dosage form are granule.
2nd, field trial embodiment
Embodiment 1：Prepare the field trial of 1 gained preparation of embodiment prevention rice seedling blight.
(1) experimental design:Experiment is with reference to NY/T 1464.14-2007《Pesticide field efficacy medicine test criterion bactericidal agent for preventing and treating Rice seedling blight》.In rice seedling blight early stage, rice shoot is uniformly spread fertilizer over the fields according to the corresponding medicament of each processing and dosage, often A plot area be 2.5 ㎡, 4 repetitions.State of an illness radix is investigated before dispenser, 14d (before transplanting) investigation prevention effect after medicine, each The 5 points of samplings of cell diagonal, every 0.1 ㎡ of investigation, record investigation total strain number, diseased plant number calculate preventive effect.Result of the test is shown in Table 3。
Table 3：Prepare the field control effectiveness test statistical form of 1 gained preparation of embodiment prevention rice seedling blight.
Experiment is set Medicament Dosage (g/ ㎡) Preventive effect (%)
Handle 1# Prepare 1 gained preparation of embodiment 15 92.4
Handle 2# 5.0% probenazole granule 20 78.2
Handle 3# 10% humic acid granule 30 42.6
Handle 4# 10000000000 cfu/ grams of bacillus subtilis granules 30 63.5
As seen from Table 3,1 gained preparation of embodiment (2.0% probenazole, 5.0% humic acid, 1,000,000,000 cfu/ is prepared Gram bacillus subtilis granule) prevention rice seedling blight, it is synergistic apparent between each ingredient, drug effect is significantly improved, is reduced Each single dose dosage.
Embodiment 2：The formulation application of 2 gained of embodiment is prepared in the field trial of prevention capsicum samping off
Experimental design：Select the serious plot of continuous cropping capsicum samping off, the early stage after capsicum tidy seedlings output, according to everywhere Corresponding medicament and dosage are managed to the uniform dispenser of each cell, plot area 2.0m2, 4 repetitions keep seedbed environment in each cell Unanimously.14d investigation results after medicine calculate preventive effect.
Result of the test is shown in Table 4.
Table 4 prepares the formulation application of 2 gained of embodiment in the field trial statistical form of prevention capsicum samping off
Experiment is set Medicament Dosage (g/ ㎡) Preventive effect (%)
Handle 1# Prepare 2 gained preparation of embodiment 15 95.6
Handle 2# 5.0% probenazole granule 20 83.2
Handle 3# 10% humic acid granule 60 53.2
Handle 4# 10000000000 cfu/ grams of bacillus subtilis granules 35 67.9
As seen from Table 4, probenazole, humic acid and bacillus subtilis mixture, it is bright to capsicum samping off synergistic effect It is aobvious, the usage amount of active ingredient had not only been reduced, but also has shown the field efficacy better than single dose.

Claims (4)

1. a kind of soil fungicides, which is characterized in that its active ingredient is by probenazole, humic acid and bacillus subtilis group Into, by 1,000,000,000,000 gemma of content/gram bacillus subtilis active compound be 100% active compound, probenazole, humic acid and withered The weight ratio of careless bacillus is 20-50:20-50：1.
2. soil fungicides according to claim 1, which is characterized in that probenazole, humic acid and bacillus subtilis The weight ratio of bacterium is 20:50:1 or 30:50:1.
3. soil fungicides according to claim 1 or 2, which is characterized in that the weight percent of probenazole is 2.0%-5.0%, the weight percent of humic acid is 2.0%-5.0%, and the weight percent of bacillus subtilis is 0.05- 0.1%.
4. according to the soil fungicides described in claim 1-3 any one, which is characterized in that prepared by the soil fungicides Agriculturally acceptable granule.
CN201810042898.7A 2018-01-17 2018-01-17 A kind of soil fungicides Pending CN108041077A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
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CN109526974A (en) * 2018-12-19 2019-03-29 江西省农业科学院植物保护研究所 A kind of composition and application method of efficient prevention and treatment rice blast

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WO2014147528A1 (en) * 2013-03-20 2014-09-25 Basf Corporation Synergistic compositions comprising a bacillus subtilis strain and a biopesticide

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109526974A (en) * 2018-12-19 2019-03-29 江西省农业科学院植物保护研究所 A kind of composition and application method of efficient prevention and treatment rice blast
CN109526974B (en) * 2018-12-19 2021-07-30 江西省农业科学院植物保护研究所 Composition for efficiently preventing and treating rice blast and application method

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