CN108013077A - A kind of microbe soil fungicide - Google Patents

A kind of microbe soil fungicide Download PDF

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Publication number
CN108013077A
CN108013077A CN201810042890.0A CN201810042890A CN108013077A CN 108013077 A CN108013077 A CN 108013077A CN 201810042890 A CN201810042890 A CN 201810042890A CN 108013077 A CN108013077 A CN 108013077A
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CN
China
Prior art keywords
bacillus subtilis
probenazole
soil
fungicide
effect
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810042890.0A
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Chinese (zh)
Inventor
程亚南
张影
刘永卓
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Henan Institute of Science and Technology
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Henan Institute of Science and Technology
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Priority to CN201810042890.0A priority Critical patent/CN108013077A/en
Publication of CN108013077A publication Critical patent/CN108013077A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12—Powders or granules
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2

Abstract

The present invention relates to a kind of microbe soil fungicide, active ingredient is made of probenazole and bacillus subtilis, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound, the weight ratio of probenazole and bacillus subtilis is 20 80:1.The mechanism of action of two active ingredients of the present invention is different, mutually mixed synergistic obvious, can effectively control soil-borne disease and delay Disease Resistance to develop, advantageously reduce applications of pesticide amount, reduce pesticide cost.

Description

A kind of microbe soil fungicide
Technical field
The present invention relates to cultural control technical field, is specifically a kind of microbe soil fungicide.
Background technology
Soil-borne disease refers to that pathogen such as fungi, bacterium, nematode and virus are lived in the soil with invalid body, and condition is suitable When from crop root or stem infringement crop caused by disease.Long-term soil continuous cropping, the raising of multiple crop index, and fertilising Unreasonable is the principal element of soil-borne disease aggravation.Samping off, root rot, damping-off are the Major Diseases of crops seedling stage, generally It can make a small number of death of seedling, cause to lack row and break ridge, it is serious that seedling can be caused to lodge in flakes death.Therefore it is badly in need of a kind of soil to kill Microbial inoculum, can effectively control the harm of soil germ, ensure the orderly progress of agricultural production.
Probenazole, English name：Probenazole, is one kind of Japanese Meiji Seika Kaisha, Ltd.'s exploitation Inducing immunity fungicide, by exciting plant to realize protection effect to immune (resistance) reaction of disease in itself, passes through plant Thing root absorption, and relatively rapidly infiltration is conducted to plant each several part.
Bacillus subtilis, English name：Bacillus subtilis, are a kind of microbial bactericides, by effectively into Divide the antibacterial material and site competition, the mode of action of induction of resistance of producing bacillus subtilis life, kill and control pathogen, from And achieve the purpose that controlling disease.After bacillus subtilis is sprayed on crop leaf, its living spores utilizes the nutrition on blade face Quickly bred on blade with moisture, capture whole blade surface rapidly, active matter of the secretion with bactericidal effect, is played effectively Competition, the effect repelled, suppress and kill germ；After being applied to soil, pathogen can be prevented to invade through competition and antagonism Plant and reaching prevents disease from occurring and promoting growth result.
In agricultural production, disease control is carried out with single medicament for a long time, germ easily produces resistance so that drug effect drops Low, prevention effect is undesirable.And the mixture of different pesticide species is used, it is the common methods of the agriculture resistance disease of prevention.Pass through Formulation of pesticide screens, and synergistic effect is significantly formulated, and can effectively improve actual prevention effect, reduces dosage, delays disease to resist Sexual development, is the important means of the disease comprehensive regulation.
At present, the bactericidal composition using probenazole and bacillus subtilis as active ingredient, applied to soil disease Prevention there is no relevant report also.
The content of the invention
It is an object of the present invention to provide a kind of microbe soil fungicide, its synergistic effect is notable, can effectively control Soil-borne disease and Disease Resistance is delayed to develop.
The technical solution adopted in the present invention is：A kind of microbe soil fungicide, active ingredient by probenazole and Bacillus subtilis forms, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound, phenyl allyloxy The weight ratio of thiazole and bacillus subtilis is 20-80:1, it is preferred that the weight ratio of probenazole and bacillus subtilis is 50:1。
The soil fungicides, content of the probenazole in fungicide are 2.0%-8.0%, bacillus subtilis Content in fungicide for 500,000,000 spores/gram~2,000,000,000 spores/gram, i.e. content 0.05- of the bacillus subtilis in fungicide 0.2%.
The soil fungicides add corresponding adjuvant, are prepared into according to method known in those skilled in the art Agriculturally acceptable granule.
The adjuvant includes filler, dispersant, wetting agent, binding agent, stabilizer or other is beneficial to active ingredient Stablize the material played with drug effect in the formulation, be all to commonly use in pesticidal preparations or allow the various composition used, have no especially Limit, specific component and dosage are determined according to recipe requirements by simple experiment.
The present invention has the following advantages and significant technique effect：
(1) mechanism of action of two active ingredients of the present invention is different, the two mixture is synergistic obvious, can greatly improve Drug effect, reduces Pesticide use amount and drug cost；Existing germ can be greatly reduced to the potential resistance risk of each single dose.
(2) soil fungicides of the present invention, are prepared into granule, easy to use, without being watered, time and labour saving.
Embodiment
Technical scheme and technique effect are described further with reference to specific embodiment, institute of the present invention The percentage stated is weight percentage.
The embodiment of the present invention is the method being combined using Toxicity Determination and field trial.
Implementation of an application example one：Probenazole compounds the Toxicity Determination to rice seedling blight with bacillus subtilis
Subjects：Rice seedling blight (Rhizoctonia solani), is gathered by field, is isolated and purified through interior.
Test method：After prerun determines the effective inhibition concentration scope of each medicament, by probenazole, bacillus subtilis And its mixed agent dilutes 5 concentration by active constituent content gradient series, is compared with sterile water.Reference《Chinese people's republicanism State agricultural industry criteria NY/T1156.2-2006》Carry out, measured using mycelial growth rate method.Various concentrations medicament is added Sterilize in culture dish, per ware 1mL, add the PDA culture medium 10mL of sterilizing fusing, be sufficiently mixed and tablet is made, then will spread cultivation Bacterium colony break into the=bacterium dish of 0.8cm, per 1, ware, often handle 4 repetitions, be placed in 25 DEG C of insulating boxs and cultivate, per 24h with ten Word interior extrapolation method measurement colony diameter 1 time, calculates each processing net growth, mycelial growth inhibition rate.
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) is converted into numerical value (x), with minimum Square law tries to achieve virulence regression equation (y=a+bx), and thus calculates the EC of every kind of medicament50Value, medicine is calculated according to the abundant methods of Sun Yun The toxicity index and co-toxicity coefficient (CTC) of agent.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100
Percentage composition+B the medicament toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed The percentage composition of B in agent
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100
When CTC≤80, then composition shows as antagonism, and as 80 ＜ CTC ＜ 120, then composition shows as being added and makees With when CTC >=120, then composition shows as synergistic effect.
Toxicity test the results are shown in Table 1.
1 bacillus subtilis of table compounds the Toxicity Determination table to rice seedling blight with probenazole
As shown in Table 1, probenazole, bacillus subtilis are to the EC of rice wilt pathogens50Respectively 5.24 μ g/mL and 3.26 μ g/mL, when probenazole with bacillus subtilis mixed ratio in 20-80:When 1, CTC is all higher than 120, has good Synergistic effect, when the weight ratio of probenazole and bacillus subtilis be 50:When 1, co-toxicity coefficient (CTC) is 206.2, is increased Effect effect is more notable, there is good guiding value in field trials.
Implementation of an application example two：Probenazole compounds the Toxicity Determination to watermelon blight with bacillus subtilis
Subjects：Watermelon blight (Fusarium oxysporum f.sp.niveum.), is gathered by field, through room Inside isolate and purify.
Test method：After prerun determines the effective inhibition concentration scope of each medicament, by probenazole, bacillus subtilis And its mixed agent dilutes 5 concentration by active constituent content gradient series, is compared with sterile water.Reference《Chinese people's republicanism State agricultural industry criteria NY/T1156.2-2006》Carry out, measured using mycelial growth rate method.Aseptically, configuration is taken Good liquid 5mL is uniformly mixed with 75mL culture mediums, and 4 drug containing tablets are made, using equivalent sterile water and culture medium mixing as Control.Cultured watermelon blight is aseptically cut into bacteria cake with the sterilization punchers of diameter 5mm from edge.With connecing For kind device by pure culture biscuits involvng inoculation in drug containing tablet center, mycelia is face-down, is placed in 25 DEG C of incubators.Depending on blank control growing state tune Look into as a result, each bacterium colony crossing method vertical survey diameter respectively once, takes its average value.Calculate mycelial growth inhibition rate, Toxicity regression formula is obtained, calculates EC50。
The synergistic effect of pharmacy mix is evaluated with the co-toxicity coefficient method (CTC) of the abundant methods of Sun Yun, i.e., CTC≤80 are that antagonism is made With 80<CTC<120 be summation action, and CTC >=120 are synergistic effect.
Toxicity test the results are shown in Table 2.
The Toxicity Determination table of 2 probenazole of table, bacillus subtilis and its mixture to watermelon blight
As shown in Table 2, probenazole, bacillus subtilis are to the EC of watermelon blight50Respectively 2.51 μ g/mL and 1.52 μ g/mL, when probenazole with bacillus subtilis mixed ratio in 20-80:When 1, CTC is all higher than 120, has good Synergistic effect, when the weight ratio of probenazole and bacillus subtilis be 50:When 1, co-toxicity coefficient (CTC) is 212.2, is increased Effect effect is more notable, there is good application value in field trials.
Implementation of an application example three：Product prepares and Field information embodiment
Embodiment 1：5% probenazole, 1,000,000,000 cfu/ grams of bacillus subtilis granule
Formula：Probenazole 5.0%, bacillus subtilis 0.1% are weighed according to quantity, and sodium lignin sulfonate 3.5% (disperses Agent), lauryl sodium sulfate 2.5% (wetting agent), polyvinyl alcohol 2.5% (binding agent), white carbon 10% (filler), diatomite (filler) complements to 100%.Each component is sufficiently mixed in the ratio of formula by more than, is crushed, and adds water to be sufficiently stirred again after soaking Uniformly, then with screw extruder pelletizer it is granulated, sieves after dry, up to 5% probenazole, 1,000,000,000 cfu/ grams of withered grass gemma Bacillus granule.
Embodiment application prevention rice seedling blight, experiment is with reference to NY/T 1464.14-2007《Pesticide field efficacy medicine test Criterion bactericidal agent for preventing and treating rice seedling blight》.In rice seedling blight early stage, by 5% probenazole 1,000,000,000 cfu/ grams it is withered Careless bacillus granule uniformly spreads fertilizer over the fields rice seedling bed, and dosage is 15g/ ㎡, and the preventive effect of 14d is 93.7% after medicine；8.0% phenyl allyloxy Thiazole granule is 15g/ ㎡ by dosage, is in kind used, and the preventive effect of 14 days is 74.3% after medicine；10000000000 cfu/ grams it is withered Careless bacillus granule, dosage are 45g/ mus, are in kind used, and the preventive effect of 14 days is 72.8% after medicine.This explanation alkene Propyl benzene thiazole is with bacillus subtilis with 50:1 weight ratio mixture synergy is obvious, can significantly improve drug effect, reduces each single dose and uses Dose.
Embodiment 2：2% probenazole, 5.0 hundred million cfu/ grams of bacillus subtilis granule
Formula:Probenazole 2.0%, bacillus subtilis 0.05%, naphthalenesulfonate formaldehyde condensation compound are weighed according to quantity 3.5% (dispersant), lauryl sodium sulfate 3.5% (wetting agent), polyvinyl alcohol 3.0% (binding agent), attapulgite 10% (filler), diatomite (filler) complement to 100%.Each component is uniformly mixed in the ratio of formula by more than, through air-flow crushing into Pulvis, adds a certain amount of water mixing extruding and makes material, 2% probenazole, 5.0 hundred million cfu/ grams of withered grass is obtained after dry screening Bacillus granule
The embodiment is applied to prevention cucumber at seedling stage root rot.By 2% probenazole, 5.0 hundred million cfu/ grams of withered grass gemma The root rot early period of origination ditch spread after cucumber emerges of bacillus granule, dosage is 2.5kg/ mus, and the preventive effect of 30d is after medicine 94.2%.5% probenazole granule dosage is 2.5kg/ mus, is in kind used, the preventive effect of 30 days is after medicine 73.6%；10000000000 cfu/ grams of bacillus subtilis granules, dosage be 6kg/ mu, in kind use, after medicine 30 days prevent Imitate as 68.4%.This explanation probenazole and bacillus subtilis rational mixture synergy are obvious, can significantly improve drug effect, reduce Drug cost.
Embodiment 3：8% probenazole, 2,000,000,000 cfu/ grams of bacillus subtilis granule
Formula:Probenazole 8.0%, bacillus subtilis 0.2% are weighed according to quantity, and carboxymethyl cellulose 3.5% (disperses Agent), neopelex 2.5% (wetting agent), polyvinyl alcohol 3.5% (binding agent), white carbon 10% (filler), kaolinite Native (filler) complements to 100%.Each component is uniformly mixed in the ratio of formula by more than, through air-flow crushing into pulvis, adds one Quantitative water mixing extruder grain, 8% probenazole, 2,000,000,000 cfu/ grams of bacillus subtilis particle is obtained after dry screening Agent
The embodiment is applied to prevention watermelon blight.By 8% probenazole, 2,000,000,000 cfu/ grams of bacillus subtilis Granule is 2.5kg/ mus by dosage, the ditch spread prevention processing after watermelon emergence, and the prevention effect of 30 days is respectively after medicine 87.6%.8.0% 5.0kg/ mus of probenazole dosage, in kind uses, and the preventive effect of 30 days is 70.4% after medicine；100 Hundred million cfu/ grams of bacillus subtilis granules, dosage are 12.5kg/ mus, are in kind used, the preventive effect of 30 days is after medicine 74.6%.This explanation probenazole and bacillus subtilis rational mixture synergy are obvious, can significantly improve drug effect, reduce medication Cost.
To sum up, a kind of microbe soil fungicide of the invention, active ingredient is by probenazole and bacillus subtilis Composition, it is not the simple superposition of two components to soil-borne disease, but has synergistic function, advantageously reduces each list The dosage of agent, improves prevention effect, there is good application value in agricultural production.

Claims (4)

  1. A kind of 1. microbe soil fungicide, it is characterised in that active ingredient is made of probenazole and bacillus subtilis, Set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound, probenazole and bacillus subtilis The weight ratio of bacterium is 20-80:1.
  2. 2. soil fungicides according to claim 1, it is characterised in that the weight of probenazole and bacillus subtilis Than for 50:1.
  3. 3. soil fungicides according to claim 1 or 2, it is characterised in that content of the probenazole in fungicide For 2.0%-8.0%, content 0.05-0.2% of the bacillus subtilis in fungicide.
  4. 4. according to the soil fungicides described in claim 1-3 any one, it is characterised in that prepared by the soil fungicides Into agriculturally acceptable granule.
CN201810042890.0A 2018-01-17 2018-01-17 A kind of microbe soil fungicide Pending CN108013077A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109526974A (en) * 2018-12-19 2019-03-29 江西省农业科学院植物保护研究所 A kind of composition and application method of efficient prevention and treatment rice blast

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* Cited by examiner, † Cited by third party
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CN105132312A (en) * 2015-08-17 2015-12-09 河南科技学院 Bacillus subtilis and application thereof as well as microbial fungicide containing bacillus subtilis and preparation method of microbial fungicide
CN105123763A (en) * 2015-08-17 2015-12-09 河南科技学院 Applications of bacillus subtilis fermentation broth, microbial fungicide containing bacillus subtilis, and preparation method of microbial fungicide
CN105685062A (en) * 2016-04-28 2016-06-22 广西南宁益土生物科技有限责任公司 Pesticide granules with probenazole and hymexazol
CN105851016A (en) * 2016-04-28 2016-08-17 广西南宁益土生物科技有限责任公司 Pesticide granules containing probenazole and metalaxyl
CN105918325A (en) * 2016-04-28 2016-09-07 广西南宁益土生物科技有限责任公司 Pesticide granule containing probenazole and metalaxyl-M
CN107041385A (en) * 2016-11-22 2017-08-15 吉林农业大学 A kind of bactericidal composition wettable powder, preparation method and its application in preventing and treating rice seedling blight

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105132312A (en) * 2015-08-17 2015-12-09 河南科技学院 Bacillus subtilis and application thereof as well as microbial fungicide containing bacillus subtilis and preparation method of microbial fungicide
CN105123763A (en) * 2015-08-17 2015-12-09 河南科技学院 Applications of bacillus subtilis fermentation broth, microbial fungicide containing bacillus subtilis, and preparation method of microbial fungicide
CN105685062A (en) * 2016-04-28 2016-06-22 广西南宁益土生物科技有限责任公司 Pesticide granules with probenazole and hymexazol
CN105851016A (en) * 2016-04-28 2016-08-17 广西南宁益土生物科技有限责任公司 Pesticide granules containing probenazole and metalaxyl
CN105918325A (en) * 2016-04-28 2016-09-07 广西南宁益土生物科技有限责任公司 Pesticide granule containing probenazole and metalaxyl-M
CN107041385A (en) * 2016-11-22 2017-08-15 吉林农业大学 A kind of bactericidal composition wettable powder, preparation method and its application in preventing and treating rice seedling blight

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109526974A (en) * 2018-12-19 2019-03-29 江西省农业科学院植物保护研究所 A kind of composition and application method of efficient prevention and treatment rice blast
CN109526974B (en) * 2018-12-19 2021-07-30 江西省农业科学院植物保护研究所 Composition for efficiently preventing and treating rice blast and application method

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Application publication date: 20180511