CN105076152B - A kind of microbicide compositions and its application - Google Patents
A kind of microbicide compositions and its application Download PDFInfo
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Abstract
The invention discloses a kind of microbicide compositions, the microbicide compositions contain active component shenqinmycin and fenoxanil, and the weight ratio of shenqinmycin and fenoxanil is 1：(0.1 100), microbicide compositions prevention effect of the invention is good, be not likely to produce the resistance to the action of a drug, to Target crops safety and to crops there is notable synergistic to act on.Microbicide compositions preventing and treating spectrum of the invention is wide, can simultaneously prevent and treat rice sheath blight disease, false smut and rice blast.
Description
Technical field
The present invention relates to agriculture field, in particular it relates to a kind of microbicide compositions and its application.
Background technology
In the plantation of paddy rice, rice blast, false smut, banded sclerotial blight have turned into the disease that China paddy fields are often sent out, retransmitted
Evil.
Rice blast also known as rice blast, are commonly called as burning pest, hang a pest, neck pest etc. pinched, by Magnaporthe grisea
(Hebert) Barr [Invisible element Pyricularia grisea (Cooke) Sace. different name Pyricularia oryzae
Cavara.] cause, there is rice blast in all places for having rice cropping of China, and knob occurs especially serious.It is popular
Time, grave illness area typically results in the paddy rice underproduction 10%~20%, have up to 40%~50%, local field even No kernels or seeds are gathered, as in a year of scarcity.
Rice green smut is fringe portion disease common on paddy rice, indivedual grain of rice of harm to the ear of rice.False smut infected seed is in blackish green
Color or black, influence the exterior quality of paddy.Ustilaginoidea virens can also produce the toxin poisonous to people and animals.In recent years, false smut
One of important disease on paddy rice is turned into.
The main rice sheath blight disease of rice sheath blight disease is main to be occurred in tiller to heading stage, harm leaf sheath, blade, stalk and rice
Fringe.Rice sheath blight disease chief destructive conducting tissue, prevents paddy rice from normally earing, and scab spreads to fringe portion, influences grain filling,
A large amount of shrivelled kernels are formed, and dead ears occurs.General morbidity can the underproduction 5%~10%, the underproduction 30%~50% when serious.
In sum, the serious stable high yield for threatening paddy rice of outburst of rice blast, false smut, banded sclerotial blight.And this three
Plant disease and easily mix generation, increase difficulty of prevention and cure, presently commercially available medicament there is no can be while prevent and treat three kinds of medicaments of disease.This
Outward, due to frequent dispenser in recent years, making the resistance of germ increases, and presently commercially available effect of chemical control declines, and dosage is big.
The content of the invention
The purpose of the present invention is to overcome the defect in prior art, there is provided a kind of prevention effect is good, be not likely to produce the resistance to the action of a drug
Bactericidal composition.
To achieve these goals, the present invention provides a kind of microbicide compositions, it is characterised in that the microbicide compositions
Containing active component shenqinmycin and fenoxanil, the weight ratio of shenqinmycin and fenoxanil is 1：(0.1-100).
On the other hand, present invention also offers the microbicide compositions in preventing and treating Pyricularia oryzae Cav, false smut and banded sclerotial blight
Application.
By above-mentioned technical proposal, shenqinmycin and fenoxanil in microbicide compositions of the invention have significant
Synergistic effect, can simultaneously improve the prevention effect to rice blast, false smut and banded sclerotial blight, to Target crops safety；Simultaneously
Due to containing non-single-activity composition, being not likely to produce the resistance to the action of a drug.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Specific embodiment
Specific embodiment of the invention is described in detail below.It should be appreciated that described herein specific
Implementation method is merely to illustrate and explain the present invention, and is not intended to limit the invention.
The present invention provides a kind of microbicide compositions, it is characterised in that the microbicide compositions contain active component Shen piperazine
The weight ratio of mycin and fenoxanil, shenqinmycin and fenoxanil is 1：(0.1-100).
According to the present invention, the active component shenqinmycin is a kind of microbial source sterilization developed by Shanghai Communications University
Agent, is to screen in the false unicellular bacterial strain of fluorescence for can be secreted from plant root soil microbiological antibiotic to obtain, its effectively into
It is divided into phenazine-1-carboxylic acid (phenazino-1-carboxylic acid).
According to the present invention, the active component fenoxanil also known as zarilamid, English name fenoxanil are melanin
Biosynthesis inhibitor, with good Inner absorptions.
According to the present invention, the weight ratio of the active component shenqinmycin and fenoxanil can become in relative broad range
Change, such as can be 1：(0.1-100), the weight ratio of preferred shenqinmycin and fenoxanil can be 1 in the present invention：(10-
30), in preferred scope, the microbicide compositions have more excellent prevention effect.
According to the present invention, the microbicide compositions also contain auxiliary agent, active component shenqinmycin and fenoxanil it is total
The percentage that weight accounts for microbicide compositions gross weight can be such as 10.5-84% in interior change in a big way, and remaining is
Auxiliary agent；The percetage by weight of preferred active component can be 20-42% in the present invention, and remaining is auxiliary agent.
According to the present invention, the auxiliary agent in the microbicide compositions can be auxiliary agent commonly used in the art, such as can be
In dispersant, wetting agent, antifreezing agent, thickener, emulsifying agent, defoamer, adhesive and solid inert carrier, in oil base or water
One or more, there is no particular restriction to this for the present invention, for example：
Dispersant in the present invention can be sodium lignin sulfonate, naphthylmethylene polymers sulfonate, polycarboxylate and polyoxy
One or more in ethene polyoxypropylene ether block copolymers.
Wetting agent in the present invention can be lauryl sodium sulfate, sldium lauryl sulfate, pull open powder and alkyl phenol polyoxy
One or more in vinethene.
Emulsifying agent in the present invention can be calcium dodecyl benzene sulfonate, Succinate sodium sulfonate, the oil of anhydrous sorbitol three
One or more in acid esters, cithrol, AEO, castor oil polyoxyethylene ether.
Thickener in the present invention can be one or two in aluminium-magnesium silicate, xanthans, organobentonite.
Antifreezing agent in the present invention can be one or more in ethylene glycol, 1,2- propane diols, glycerine and urea.
Binding agent in the present invention can be starch, gelatin, Arabic gum, polyvinylpyrrolidone, methylcellulose, Portugal
One or more in grape sugar, sucrose.
Defoamer in the present invention can be one or more in silicone oil, higher alcohol, mineral oil and vegetable oil.
Oil base in the present invention is one or more in methyl oleate, paraffin oil, white oil, soybean oil.
Solid inert carrier in the present invention can be the one kind or many in kaolin, diatomite, white carbon, sepiolite
Kind.
Composition of the invention can be provided with final product form, i.e., each component is blended in composition, it is also possible to every kind of
Composition is provided in the form of independent preparation, bucket is directly carried out when using and is mixed.
In the present invention, according to the difference of selected auxiliary agent, microbicide compositions can be prepared into different dosage forms, such as
Suspending agent, oil-suspending agent, water dispersible granules or wettable powder can be prepared into, wherein, in addition to active component, other are helped
The consumption of agent is simultaneously had no special requirements, and can be carried out according to conventional proportions in this area, such as following formulation is removed and contains 10.5-84
Outside the active component of weight %, other components are：
Suspending agent typically contains 2-5 weight % dispersants, 0.5-2 weight % wetting agents, 2-10 weight % antifreezing agents, 0-5
Weight % thickeners, 0-1 weight % defoamers, balance of water.Suspending agent generally need wet pulverizing with obtain stabilization, can flow
Dynamic, easily measurement volume suspension liquid.
Oil-suspending agent typically contains 0.5-5 weight % dispersants, 5-10 weight % emulsifying agents, 0.1-5 weight % thickeners,
Balance of oil base.Oil-suspending agent generally needs wet pulverizing to obtain stabilization, flowable, easy measurement volume oil suspension body.
Wettable powder typically contains 0.5-10 weight % dispersants, 0.5-5 weight % wetting agents, balance of solid, inert
Carrier.Wettable powder generally needs dry pulverization process to obtain free flowable powdery solid.
Water dispersible granules typically contain 0.5-10 weight % dispersants, 0.5-5 weight % wetting agents, 0-10 weight % other
Additive such as stabilizer, adhesive, such as balance of solid inert carrier, kaolin.Water dispersible granules are usually made 10-100
Mesh international standard sieve mesh (1676-152 μm) particle, can be made by mist projection granulating, extruder grain, high speed rotating granulation, fluid bed
It is prepared by the methods such as grain.
One kind of the invention is preferred embodiment that the microbicide compositions are prepared as oil-suspending agent, wherein, auxiliary agent
It is at least one in AEO, calcium dodecyl benzene sulfonate, bentonite and methyl oleate.
It will be understood by those skilled in the art that the various formulations in the present invention can be according to reality when actually used
Dilution is needed to use.
On the other hand, present invention also offers bactericide as described above in preventing and treating crops rice blast, false smut and line
Application in rot, wherein, crops can be paddy rice.
Wherein, consumption of the above-mentioned microbicide compositions every mu of ground is not particularly limited, specifically can according to effectively into
The active constituent content regulation of the mu consumption and preparation that divide, for example, can make every mu of total amount of application of the active component of ground be 1-
100g。
Be further elaborated the present invention by the following examples.
In following testing example, wherein, testing example 1 is that the synergistic effect of microbicide compositions of the present invention is entered
Row biological detection, altogether including three tests, respectively testing example 1.1：Rice blast Toxicity Determination, test implementation
Example 1.2：Rice green smut Toxicity Determination, testing example 1.3：Rice sheath blight disease Toxicity Determination.Biological detection is adopted
With Sun Yunpei (Sun Y-P) method, by calculating co-toxicity coefficient (co-toxicity coefficient, CTC), the present invention is evaluated
Microbicide compositions in two kinds of active components mixed effect.It is specific as follows：Evaluation criterion：When CTC >=120, show as increasing
Effect is acted on；When CTC≤80, antagonism is shown as；80 ＜ CTC ＜ 120, show as summation action.
Specific formula for calculation is：
Co-toxicity coefficient (CTC)=[the actual toxicity index of mixture (ATI)/mixture theoretical toxicity index (TTI)] × 100；
The actual toxicity index of mixture (ATI)=(LC of standard agent50The LC of/mixture50)×100；
There is the TI × mixture of the content+B agent of A in the TI of mixture theoretical toxicity index (TTI)=A agent × mixture active ingredient
The content of B in effect composition；
Toxicity index (TI)=(LC of standard agent50The LC of/reagent agent50(one kind in A agent, B agent is made) × 100
It is standard agent)；
A agent, B agent are respectively two kinds of mutually mixed active components, and mixture is the mixture after A agent is mutually mixed with B agent.
Testing example 2 is that microbicide compositions of the present invention are used for efficacy testing when in field test.
Testing example 1
In the examples below, A agent is shenqinmycin active compound (95%), and B agent is fenoxanil active compound (95%), selects A
Agent by A agent and B agent respectively on the basis of the weight of respective active ingredient, is configured to mixture as shown in table 1 as standard agent
1 to 7.
The rice blast Toxicity Determination of testing example 1.1
Experiment target：Rice Pyricularia grisea, is provided by agricultural college of China Agricultural University laboratory.
Medicament is prepared：7 kinds of mixtures and 2 kinds of single doses as shown in table 1 are accurately weighed respectively, and every kind of medicament is configured with acetone
Into mother liquor, and mother liquor is diluted the serial medicament for turning into and having finite concentration gradient with the aqueous solution of the Tween-80 of 0.1 weight %.
Culture medium：Potato culture (PDA).Weigh 200g potatos to be placed in the beaker of 2500mL, add 1000mL
Distilled water boils 30min, filtering, adds 20g glucose and 20g agar, and 1000mL is complemented to distilled water after thawing, and packing is gone out
Bacterium (126 DEG C, 25min), it is standby.
Chemicals treatment：Under aseptic technique, the advance sterilising medium for melting is quantitatively adding according to test process
To in aseptic conical flask, then quantitatively draw above-mentioned liquid successively from low concentration to high concentration, be separately added into above-mentioned conical flask, fill
Divide and shake up.Then equivalent is poured into 5 culture dishes of a diameter of 9cm, is made the pastille flat board of various concentrations.Separately set without medicament
Treatment make blank (plus Tween-80 aqueous solution of 0.1 weight % of equivalent) and comprise only shenqinmycin or rice blast acyl
The effect comparison of amine single component, each treatment is repeated 5 times.
Inoculation：Under aseptic technique, the rice blast opportunistic pathogen sterilization punchers of diameter 5mm of 3 days will be cultivated
Bacteria cake is cut from colony edge, with inoculator by pure culture biscuits involvng inoculation in pastille flat board center, mycelia faces up, and each culture dish puts 1
Individual bacteria cake, each concentration is repeated 5 times, and blank is equally processed as stated above.Postvaccinal culture dish is placed in (25 ± 1)
Observation is cultivated in DEG C constant incubator.
Investigation and calculating：According to rice blast fungus colony growth situation in blank culture dish, bacterium colony to be compareed is straight
When path length is to more than plate diameter 2/3, with kind of calliper colony diameter (mm).Each bacterium colony is straight with crossing method vertical survey
Each 1 time of footpath, takes its average value.Mycelial growth inhibition rate is calculated, further according to log concentration-mycelial growth inhibition rate niqueMin analysis
(Bliss) method, concentration (EC in being obtained virulence regression equation and suppressed with the data processing softwares of DPS 200050) (half effect is dense
Degree, causes that study subject 50% is individual to produce a kind of drug dose of specific effect) value.Then according to Sun Yunpei (Sun-Y-
P) method introduced, obtains the co-toxicity coefficient (CTC) of different ratio mixed agent, the results are shown in Table 1.
Bacterium colony increases diameter=colony diameter-bacteria cake diameter
Table 1
| Medicament | EC50(mg/L) | ATI | TTI | Co-toxicity coefficient (CTC) |
| Shenqinmycin (A) | 0.12 | 100.00 | -- | -- |
| Fenoxanil (B) | 5.96 | 2.01 | -- | -- |
| (the A of mixture 1：B=1：100) | 3.09 | 3.88 | 2.98 | 130.13 |
| (the A of mixture 2：B=1：50) | 2.10 | 5.71 | 3.93 | 145.09 |
| (the A of mixture 3：B=1：30) | 0.97 | 12.38 | 5.17 | 239.27 |
| (the A of mixture 4：B=1：20) | 0.70 | 17.04 | 6.68 | 255.04 |
| (the A of mixture 5：B=1：10) | 0.50 | 24.17 | 10.92 | 221.33 |
| (the A of mixture 6：B=1：1) | 0.16 | 76.64 | 51.01 | 150.26 |
| (the A of mixture 7：B=10：1) | 0.10 | 123.97 | 91.09 | 136.09 |
The rice green smut Toxicity Determination of testing example 1.2
Experiment target：Rice green smut germ, is provided by agricultural college of China Agricultural University laboratory.
Medicament is prepared：7 kinds of mixtures and 2 kinds of single doses as shown in table 1 are accurately weighed respectively, and every kind of medicament is configured with acetone
Into mother liquor, and mother liquor is diluted the serial medicament for turning into and having finite concentration gradient with the aqueous solution of the Tween-80 of 0.1 weight %.
Culture medium：Potato culture (PDA).Weigh 200g potatos to be placed in the beaker of 2500mL, add 1000mL
Distilled water boils 30min, filtering, adds 20g glucose and 20g agar, and 1000mL is complemented to distilled water after thawing, and packing is gone out
Bacterium (126 DEG C, 25min), it is standby.
Chemicals treatment：Under aseptic technique, the advance sterilising medium for melting is quantitatively adding according to test process
To in aseptic conical flask, then quantitatively draw above-mentioned liquid successively from low concentration to high concentration, be separately added into above-mentioned conical flask, fill
Divide and shake up.Then equivalent is poured into 5 culture dishes of a diameter of 9cm, is made the pastille flat board of various concentrations.Separately set without medicament
Treatment make blank (plus Tween-80 aqueous solution of 0.1 weight % of equivalent) and comprise only shenqinmycin or rice blast acyl
The effect comparison of amine single component, each treatment is repeated 5 times.
Inoculation：Under aseptic technique, the rice blast opportunistic pathogen sterilization punchers of diameter 5mm of 3 days will be cultivated
Dish bacterium is prepared from colony edge, and bacterium dish is accessed on pastille flat board, each concentration is repeated 5 times, blank is equally by above-mentioned
Method treatment.Postvaccinal culture dish is placed in dark culturing 14 days in (28 ± 1) DEG C constant incubator.
Investigation and calculating：According to rice blast fungus colony growth situation in blank culture dish, bacterium colony to be compareed is straight
When path length is to more than plate diameter 2/3, with kind of calliper colony diameter (mm).Each bacterium colony is straight with crossing method vertical survey
Each 1 time of footpath, takes its average value.Mycelial growth inhibition rate is calculated, further according to log concentration-mycelial growth inhibition rate niqueMin analysis
(Bliss) method, concentration (EC in being obtained virulence regression equation and suppressed with the data processing softwares of DPS 200050) (half effect is dense
Degree, causes that study subject 50% is individual to produce a kind of drug dose of specific effect) value.Then according to Sun Yunpei (Sun-Y-
P) method introduced, obtains the co-toxicity coefficient (CTC) of different ratio mixed agent, the results are shown in Table 2.
Bacterium colony increases diameter=colony diameter-bacteria cake diameter
Table 2
| Medicament | EC50(mg/L) | ATI | TTI | Co-toxicity coefficient (CTC) |
| Shenqinmycin (A) | 0.15 | 100 | -- | -- |
| Fenoxanil (B) | 10.92 | 1.37 | -- | -- |
| (the A of mixture 1：B=1：100) | 5.06 | 2.96 | 2.35 | 126.07 |
| (the A of mixture 2：B=1：50) | 3.25 | 4.61 | 3.31 | 139.33 |
| (the A of mixture 3：B=1：30) | 1.53 | 9.83 | 4.56 | 215.82 |
| (the A of mixture 4：B=1：20) | 1.02 | 14.71 | 6.07 | 242.35 |
| (the A of mixture 5：B=1：10) | 0.66 | 22.86 | 10.34 | 221.06 |
| (the A of mixture 6：B=1：1) | 0.18 | 85.14 | 50.69 | 167.98 |
| (the A of mixture 7：B=10：1) | 0.12 | 123.37 | 91.03 | 135.52 |
The rice sheath blight disease Toxicity Determination of testing example 1.3
Experiment target：Rice sheath blight disease germ, is provided by agricultural college of China Agricultural University laboratory.
Medicament is prepared：7 kinds of mixtures and 2 kinds of single doses as shown in table 1 are accurately weighed respectively, and every kind of medicament is configured with acetone
Into mother liquor, and mother liquor is diluted the serial medicament for turning into and having finite concentration gradient with the aqueous solution of the Tween-80 of 0.1 weight %.
Culture medium：Potato culture (PDA).Weigh 200g potatos to be placed in the beaker of 2500mL, add 1000mL
Distilled water boils 30min, filtering, adds 20g glucose and 20g agar, and 1000mL is complemented to distilled water after thawing, and packing is gone out
Bacterium (126 DEG C, 25min), it is standby.
Chemicals treatment：Under aseptic technique, the advance sterilising medium for melting is quantitatively adding according to test process
To in aseptic conical flask, then quantitatively draw above-mentioned liquid successively from low concentration to high concentration, be separately added into above-mentioned conical flask, fill
Divide and shake up.Then equivalent is poured into 5 culture dishes of a diameter of 9cm, is made the pastille flat board of various concentrations.Separately set without medicament
Treatment make blank (plus Tween-80 aqueous solution of 0.1 weight % of equivalent) and comprise only shenqinmycin or rice blast acyl
The effect comparison of amine single component, each treatment is repeated 5 times.
Inoculation：Under aseptic technique, the rice banded sclerotial blight pathogen sterilization punchers of diameter 5mm of 3 days will be cultivated
Bacteria cake is cut from colony edge, with inoculator by pure culture biscuits involvng inoculation in pastille flat board center, mycelia faces up, and each culture dish puts 1
Individual bacteria cake, each concentration is repeated 5 times, and blank is equally processed as stated above.Postvaccinal culture dish is placed in (25 ± 1)
Observation is cultivated in DEG C constant incubator.
Investigation and calculating：According to Rhizoctonia solani Kuhn colony growth situation in blank culture dish, bacterium colony to be compareed is straight
When path length is to more than plate diameter 2/3, with kind of calliper colony diameter (mm).Each bacterium colony is straight with crossing method vertical survey
Each 1 time of footpath, takes its average value.Mycelial growth inhibition rate is calculated, further according to log concentration-mycelial growth inhibition rate niqueMin analysis
(Bliss) method, concentration (EC in being obtained virulence regression equation and suppressed with the data processing softwares of DPS 200050) (half effect is dense
Degree, causes that study subject 50% is individual to produce a kind of drug dose of specific effect) value.Then according to Sun Yunpei (Sun-Y-
P) method introduced, obtains the co-toxicity coefficient (CTC) of different ratio mixed agent, the results are shown in Table 3.
Bacterium colony increases diameter=colony diameter-bacteria cake diameter
Table 3
| Medicament | EC50(mg/L) | ATI | TTI | Co-toxicity coefficient (CTC) |
| Shenqinmycin (A) | 0.091 | 100.00 | -- | -- |
| Fenoxanil (B) | 7.83 | 1.16 | -- | -- |
| (the A of mixture 1：B=1：100) | 3.43 | 2.65 | 2.14 | 123.85 |
| (the A of mixture 2：B=1：50) | 2.05 | 4.45 | 3.10 | 143.42 |
| (the A of mixture 3：B=1：30) | 0.95 | 9.58 | 4.35 | 220.16 |
| (the A of mixture 4：B=1：20) | 0.60 | 15.20 | 5.87 | 258.97 |
| (the A of mixture 5：B=1：10) | 0.39 | 23.60 | 10.15 | 232.58 |
| (the A of mixture 6：B=1：1) | 0.10 | 93.92 | 50.58 | 185.69 |
| (the A of mixture 7：B=10：1) | 0.06 | 142.84 | 91.01 | 156.94 |
From table 1 to 3 as can be seen that the weight ratio of the active component shenqinmycin and fenoxanil in bactericide is 1：
When (0.1-100), synergistic function is capable of achieving to rice blast, false smut and banded sclerotial blight germ；And when its weight ratio is 1：
When (10-30), co-toxicity coefficient (CTC) is more than 200, and synergistic effect is more notable.
Testing example 2
First according to the recipe configuration bactericide in following examples and comparative example, it is then tested in field control paddy rice rice
Effect in seasonal febrile diseases, false smut and banded sclerotial blight.Wherein, shenqinmycin is purchased from Shanghai Nongle Biological Product Co., Ltd.；Rice
Pest acid amides is purchased from Jiangsu Fengdeng Pesticide Co., Ltd.；Sodium lignin sulfonate is purchased from the Gaotang East China limited public affairs of lignin
Department；Xanthans is purchased from Zibo Ye Sheng Industrial Co., Ltd.s；Aluminium-magnesium silicate is purchased from Jinan Yongtai Chemical Co., Ltd.；Organosilicon froth breaking
Agent is purchased from Shanghai Mitugao New Material Group；AEO is purchased from Nanjing Teva-Chem. Co., Ltd.；Dodecyl
Benzene sulfonic acid calcium is purchased from Nanjing Teva-Chem. Co., Ltd.；Bentonite is purchased from Xinyang Xin Ding mining industry Co., Ltd；Methyl oleate is purchased
From Cangzhou Dayang Chemical Co., Ltd.；Naphthylmethylene polymers sulfonate is purchased from Shanghai Shi great macromolecular materials Co., Ltd；
Lauryl sodium sulfate is purchased from Beijing Tai Bohong Chemical Co., Ltd.s；Starch is purchased from Wujiang Tianxiang Chemical Co., Ltd.；Kaolinite
Soil is purchased from village Chen Xing kaolin processing factory of family；Polycarboxylate is purchased from Shanghai Shi great macromolecular materials Co., Ltd；Pull open powder is purchased from
Beijing Tai Bohong Chemical Co., Ltd.s；White carbon is purchased from Shandong Lian Ke white carbons Co., Ltd；Diatomite is purchased from Henan Wan Jiang rings
Protect Science and Technology Ltd..
Embodiment 1
21% shenqinmycin fenoxanil suspending agent
The weight % of shenqinmycin 1, the weight % of fenoxanil 20, the weight % of sodium lignin sulfonate 4, the weight % of xanthans 0.2,
The weight % of aluminium-magnesium silicate 0.5, the weight % of organic silicon defoamer 0.1, water are added to 100 weight %.
Embodiment 2
21% shenqinmycin fenoxanil oil-suspending agent
The weight % of shenqinmycin 1, the weight % of fenoxanil 20, the weight % of AEO 10, detergent alkylate
The weight % of sulfoacid calcium 5, the weight % of bentonite 0.2, methyl oleate are added to 100 weight %.
Embodiment 3
21% shenqinmycin fenoxanil suspending agent
The weight % of shenqinmycin 1, the weight % of fenoxanil 20, the weight % of naphthylmethylene polymers sulfonate 8, dodecyl sulphur
The weight % of sour sodium 4, the weight % of starch 5, kaolin are added to 100 weight %.
Embodiment 4
21% shenqinmycin fenoxanil suspending agent
The weight % of shenqinmycin 1, the weight % of fenoxanil 20, the weight % of polycarboxylate 5, the weight % of pull open powder 2, white carbon
0.5 weight %, diatomite is added to 100 weight %.
Embodiment 5
10.5% shenqinmycin fenoxanil oil-suspending agent
The weight % of shenqinmycin 0.5, the weight % of fenoxanil 10, the weight % of AEO 10, dodecyl
The weight % of benzene sulfonic acid calcium 5, the weight % of bentonite 0.2, methyl oleate are added to 100 weight %.
Embodiment 6
42% shenqinmycin fenoxanil oil-suspending agent
The weight % of shenqinmycin 2, the weight % of fenoxanil 40, the weight % of AEO 10, detergent alkylate
The weight % of sulfoacid calcium 5, the weight % of bentonite 0.2, methyl oleate are added to 100 weight %.
Embodiment 7
84% shenqinmycin fenoxanil oil-suspending agent
The weight % of shenqinmycin 4, the weight % of fenoxanil 80, the weight % of AEO 10, detergent alkylate
The weight % of sulfoacid calcium 5, the weight % of bentonite 0.2, methyl oleate are added to 100 weight %.
Embodiment 8
21.7% shenqinmycin fenoxanil oil-suspending agent
The weight % of shenqinmycin 0.7, the weight % of fenoxanil 30, the weight % of AEO 10, dodecyl
The weight % of benzene sulfonic acid calcium 5, the weight % of bentonite 0.2, methyl oleate are added to 100 weight %.
Embodiment 9
22% shenqinmycin fenoxanil oil-suspending agent
The weight % of shenqinmycin 2, the weight % of fenoxanil 20, the weight % of AEO 10, detergent alkylate
The weight % of sulfoacid calcium 5, the weight % of bentonite 0.2, methyl oleate are added to 100 weight %.
Comparative example 1
10.5% shenqinmycin oil-suspending agent
The weight % of shenqinmycin 10.5, the weight % of fenoxanil 10, the weight % of AEO 10, dodecyl
The weight % of benzene sulfonic acid calcium 5, the weight % of bentonite 0.2, methyl oleate are added to 100 weight %.
Comparative example 2
10.5% fenoxanil oil-suspending agent
The weight % of fenoxanil 10.5, the weight % of AEO 10, the weight % of calcium dodecyl benzene sulfonate 5,
The weight % of bentonite 0.2, methyl oleate is added to 100 weight %.
Experiment is located in the farmland covered by contract of the peasant household of Nanchang City, Jiangxi Province Jinxian County one, and experimental plot is continuous cropping rice field, soil pH value
5.6, middle fertility.Experimental cultivar is five Feng You T025.Application method is uniform with the sharp agriculture hand compression sprayer of HD-400 types
Spraying, blank spray equivalent clear water.
Efficacy survey is carried out after medicine after 14 days.Investigation method：The 5 points of samplings of each cell diagonal, do not put 5 Cong Shui of investigation
Rice, each cell investigates 25 clumps, record total strain number, diseased plant number and sick series altogether.
Rice sheath blight disease severity Scaling standard：0 grade：Complete stool is disease-free；1 grade：4th blade and its following leaf sheath, blade hair
Sick (being the 1st blade with sword-like leave)；3 grades：3rd blade and its following leaf sheath, blade morbidity；5 grades：2nd blade and its following
Leaf sheath, blade morbidity；7 grades：Sword-like leave blade and its following leaf sheath, blade morbidity；9 grades：Complete stool falls ill, withered ahead of time.
Rice green smut grade scale：0 grade：Do not fall ill；1 grade：Per 1 rice song of fringe；2 grades：Per 2 rice songs of fringe；3 grades：Often
3~5 rice songs of fringe；4 grades：Per 6~9 rice songs of fringe；5 grades：It is bent per more than 10 rice of fringe.Rice blast grade scale：0 grade：
It is disease-free；1 grade：Loss less than 5% (morbidity of indivedual branch stalks) per fringe；3 grades：(1/3 or so the branch stalk hair of loss 6%~20% per fringe
Disease)；5 grades：Loss 21%~50% per fringe (fringe neck or main shaft are fallen ill, and grain half is flat)；7 grades：51%~70% (fringe is lost per fringe
Neck is fallen ill, big portion's shrivelled kernel)；9 grades：Loss 71%~100% per fringe (fringe neck is fallen ill, and causes dead ears).
Disease index and prevention effect are calculated by formula (1) and (2), prevention effect is shown in Table 4.
Formula (1)
Formula (2)
Table 4
As can be seen from Table 4, the microbicide compositions that the present invention is provided are relative to oligomict shenqinmycin or rice blast acyl
Amine, with synergistic effect, can improve prevention effect, be not likely to produce the resistance to the action of a drug, and preventing and treating spectrum is wide, can simultaneously prevent and treat rice banded sclerotial blight
Disease, false smut and rice blast.
Simultaneously as can be seen that when the gross weight of active component shenqinmycin and fenoxanil is microbicide compositions gross weight
20-42% when, can further improve prevention effect.
The preferred embodiment of the present invention described in detail above, but, the present invention is not limited in above-mentioned implementation method
Detail, in range of the technology design of the invention, various simple variants can be carried out to technical scheme, this
A little simple variants belong to protection scope of the present invention.
It is further to note that each particular technique feature described in above-mentioned specific embodiment, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy is no longer separately illustrated.
Additionally, can also be combined between a variety of implementation methods of the invention, as long as it is without prejudice to originally
The thought of invention, it should equally be considered as content disclosed in this invention.
Claims (10)
1. a kind of microbicide compositions, it is characterised in that the microbicide compositions contain and are made up of shenqinmycin and fenoxanil
Active component, the weight ratio of shenqinmycin and fenoxanil is 1：(0.1-100).
2. microbicide compositions according to claim 1, wherein, the weight ratio of the shenqinmycin and fenoxanil is 1：
(10-30)。
3. microbicide compositions according to claim 1, wherein, the active component shenqinmycin and fenoxanil it is total
Weight is the 10.5-84% of microbicide compositions gross weight.
4. microbicide compositions according to claim 1, wherein, the active component shenqinmycin and fenoxanil it is total
Weight is the 20-42% of microbicide compositions gross weight.
5. microbicide compositions according to claim 1, wherein, the microbicide compositions also contain auxiliary agent.
6. microbicide compositions according to claim 5, wherein, the auxiliary agent is dispersant, wetting agent, emulsifying agent, increasing
At least one in thick dose, antifreezing agent, binding agent, defoamer, oil-based media, water and solid inert carrier.
7. microbicide compositions according to claim 6, wherein, dispersant is sodium lignin sulfonate, naphthalene in the auxiliary agent
At least one in formaldehyde condensation products sulfonate, polycarboxylate and polyoxyethylene poly-oxygen propylene aether block copolymer；Wetting agent is
At least one in lauryl sodium sulfate, sldium lauryl sulfate, pull open powder and APES；Emulsifying agent is 12
Alkyl benzene calcium sulfonate, Succinate sodium sulfonate, sorbitan trioleate, cithrol, fatty alcohol polyoxy second
One or more in alkene ether and castor oil polyoxyethylene ether；Oil-based media is in methyl oleate, paraffin oil, white oil and soybean oil
At least one；Solid inert carrier is at least one in kaolin, diatomite, white carbon and sepiolite.
8. microbicide compositions according to claim 5, wherein, the auxiliary agent is AEO, dodecane
At least one in base benzene sulfonic acid calcium, bentonite and methyl oleate.
9. the microbicide compositions in claim 1-8 described in any one are withered in preventing and treating crops rice blast, false smut and line
Application in disease.
10. the application of microbicide compositions according to claim 9, wherein, the crops are paddy rice.
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