CN105230622A - Pesticide composition containing physcion and kasugamycin - Google Patents

Pesticide composition containing physcion and kasugamycin Download PDF

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CN105230622A
CN105230622A CN201510725660.0A CN201510725660A CN105230622A CN 105230622 A CN105230622 A CN 105230622A CN 201510725660 A CN201510725660 A CN 201510725660A CN 105230622 A CN105230622 A CN 105230622A
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physcion
kasugamycin
kasugarnycin
composition
preparation
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CN105230622B (en
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陈劲礼
李新杰
刘晓鹏
梁明龙
许丽娟
刘晓静
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JIANGMEN PLANT PROTECTION CO Ltd
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JIANGMEN PLANT PROTECTION CO Ltd
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Abstract

The invention belongs to the field of pesticides, particularly a pesticide composition containing physcion and kasugamycin. The composition is compounded by adding auxiliary agentsinto two main ingredients: physcion and kasugamycin, wherein the form of the preparation is water aqua, a suspending agent or wettable powder. The composition comprises 0.1-2% of physcion, 0.5-10% of kasugamycin and the balance of auxiliary agents. According to the composition, the physcion can be high content concentrate or a preparation of physcion; the kasugamycin can be a technical material or a preparation of kasugamycin or a compound indicating the active ingredients by a kasugamycin hydrochloride hydrate. The composition, by virtue of obvious synergistic interaction after physcion and kasugamycin are compounded, is widely used for preventing and treating various bacteria of crops, plays both protecting and treating roles on crops, and is safe to human and the environment and is not easy to generate drug resistance to bacteria, so that the pesticide composition is an ideal pollution-free bactericide.

Description

A kind of composition pesticide containing Physcion and kasugarnycin
Technical field
The invention belongs to pesticide field, be specifically related to a kind of composition pesticide containing Physcion and kasugarnycin.
Background technology
Physcion is a kind of botanical pesticide extracted from the root, stem of Chinese herbal medicine polygonaceae plant rheum officinale, belongs to anthraquinone analog compound.This composition is a kind of Secondary metabolites, has interior absorption, has the effect of controlling plant diseases.Its mechanism of action is mainly oxidation, the dehydrogenation of sugar and the glycometabolism intermediate product suppressing germ, the synthesis of Profilin matter and nucleic acid, show through toxicity mensuration and field control effectiveness test result, there are good suppression and control efficiency to crop powdery mildew, also have good preventive effect to downy mildew, gray mold and anthracnose etc. simultaneously.Also there are some researches show, Physcion can affect the formation of hypha,hyphae growth and haustorium, has induced resistance effect to vegetable category simultaneously.
Kasugarnycin is a kind of low toxicity, systemic fungicide, is the special antibiotic of control rice blast.Sterilization mechanism is the protein synthesis affecting germ, suppresses hyphal elongation and causes cell granulations.Except control rice blast, there is good preventive and therapeutic effect to multiple fungi bacteriosises such as cucumber anthracnose, bacterial angular leaf spot, cladosporium leaf and fruit mould of tomato, oranges and tangerines gummosiss, be widely used at present.
Present inventor finds, Physcion and kasugarnycin is carried out Combined machining and becomes pesticidal preparations to use, and has outstanding synergistic effect, so complete the present invention in indoor and the control of field to a lot of germ.Further, according to data-searching, do not find so far Physcion and kasugarnycin to be carried out the composite open report being processed into pesticidal preparations and also using.
Summary of the invention
The object of the present invention is to provide a kind of composition pesticide containing Physcion and kasugarnycin, it has excellent synergy control efficiency.
Another object of the present invention various preparations being to provide described composition pesticide and preparation method thereof.
Contain a composition pesticide for Physcion and kasugarnycin, it is characterized in that, weight percentage, Physcion accounts for 0.1-2% in the composition, and kasugarnycin accounts for 0.5-10%, and all the other are auxiliary agent.
Preferably, weight percentage, Physcion accounts for 0.2-1% in the composition, and kasugarnycin accounts for 1-6%, and all the other are auxiliary agent.
Preferably, Physcion can be the female medicine of its high-load or the preparation of itself;
Preferably, kasugarnycin can be former medicine or the preparation of itself, or indicates the compound of active ingredient with kasugamycin hydrochloride hydrate.
Preferably, above-mentioned composition pesticide mixture can become aqua, suspending agent or wetting powder.
Above-mentioned auxiliary agent is:
When preparing aqua, its auxiliary agent is:
The wetting agent of 0.2-20% by weight, wetting agent is alkyl benzene sulfonate, alkyls sulfonate, dialkyl succinylsuccinate ester class sulfonate, alkyl naphthalene sulfonic acid salt, fatty acid amide taurine salt, phenethyl phenolic ether class sulfonate, alkoxy polyoxy ethers sulfonate, alkyl phosphate class phosphate, polyoxyethylene carboxylate class phosphate, alkyl polyoxyethylene ether class phosphate, alkylphenol polyoxyethylene phosphoric acid salt, aralkyl phenol polyethenoxy ethers phosphate, alkylphenol polyoxyethylene class sulphate, fatty alcohol-polyoxyethylene ether, aliphatic acid polyethenoxy ether class, castor oil oxirane and derivative, sorbitan fatty acid and oxirane thereof, alkylphenol polyoxyethylene and formaldehyde condensation products thereof, one or more in Fluhyzon APEO and formaldehyde condensation products thereof,
The cosolvent of 0-15% by weight, cosolvent is one or more in methyl alcohol, acetonitrile, acetone, ethylene glycol, glycerine, DMF, DMA, 1-METHYLPYRROLIDONE, DMSO;
The preservative of 0.1-2% by weight, preservative is one or more in formaldehyde, isothiazolinone, Buddhist nun uncle tortoise beetle ester, potassium sorbate, Sodium Benzoate;
The antifreezing agent of 1-8% by weight, antifreezing agent is one or more in ethylene glycol, propane diols, glycerine, urea;
The pH value regulator of 0-1% by weight, pH value regulator is one or more in sodium hydroxide, ammonium hydroxide, acetic acid, hydrochloric acid, phosphoric acid;
Finally mend to 100% by deionized water.
When preparing suspending agent, its auxiliary agent comprises:
The wetting agent of 0.2-10% by weight, wetting agent is Negel, lauryl sodium sulfate, polyoxyethylene nonylphenol ether, fatty alcohol-polyoxyethylene ether, fatty alcohol ethylene oxide adduct sulfonate, alkylaryl APEO, lauryl alcohol polyethylene glycol oxide base ether sodium sulfate, alkyl or aralkyl phenol polyethenoxy base ether sodium sulfate, alkyl naphthalene formaldehyde condensate sulfonates, alkylphenol formaldehyde condensation product ethylene oxide adduct sulfonate, alkylphenol-polyethenoxy base ether formaldehyde condensation products, polyoxyethylene polyoxypropylene base ether block copolymers, lignin and derivative sulfonate thereof, the alkylaryl sulfonates of polymerization, polycarboxylate, fatty alcohol ethylene oxide adduct phosphate, alkylphenol-polyethenoxy base ether phosphate (ester), agriculture breast 0201, agriculture breast 1602, agriculture breast 602, Tween-20, one or more in Tween-80,
The dispersant of 0.3-10% by weight, dispersant is lignosulfite and solid content thereof, lignin and derivative sulfonate thereof, naphthalene or alkyl naphthalene formaldehyde condensation product and salt thereof, fatty alcohol ethylene oxide adduct sulfonate, alkylphenol-polyethenoxy base ether sulfonate, polycarboxylate, fatty alcohol ethylene oxide adduct phosphate, alkylphenol-polyethenoxy base ether sulfonate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, polyoxyethylene poly-oxygen propylene aether block copolymer, carboxymethyl cellulose, polyvinyl alcohol, polyvinylpyrrolidone, one or more in polyethylene glycol,
The tackifier of 0.1-5% by weight, tackifier are one or more in gum Arabic, xanthan gum, methylcellulose, carboxymethyl cellulose, polyvinyl alcohol, polyvinylpyrrolidone, Sodium Polyacrylate, gaseous silicon oxide, Magnesiumaluminumsilicate;
The preservative of 0.1-2% by weight, preservative is one or more in formaldehyde, isothiazolinone, Buddhist nun uncle tortoise beetle ester, potassium sorbate, Sodium Benzoate;
The antifreezing agent of 1-8% by weight, antifreezing agent is one or more in ethylene glycol, propane diols, glycerine, urea;
The defoamer of 0-1% by weight, defoamer is organic silicone, C 10-20one or more in saturated fat acids, iodoxy amine, ester ethers;
The pH value regulator of 0-1% by weight, pH value regulator is one or more in sodium hydroxide, ammonium hydroxide, acetic acid, hydrochloric acid, phosphoric acid;
Finally mend to 100% by deionized water.
When preparing wetting powder, its auxiliary agent comprises:
The wetting agent of 1-15% by weight, wetting agent is aliphatic alcohol sulfate, alkylol polyoxyethylene groups ether sodium sulfate, alkylphenol-polyethenoxy base ether sodium sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, sodium alkyl sulfonate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, dialkyl succinylsuccinate sulfonate, monoalkyl phenyl polyoxyethylene groups succinic acid sodium sulfonate, fatty alcohol ethylene oxide adduct sulfonate, alkylphenol formaldehyde condensation product ethylene oxide adduct sulfonate, alkyl amide taurate, alkylphosphonic, carboxylate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester, sorbitan fatty acid ester polyoxyethylene ether, polyoxyethylene poly-oxygen propylene aether block copolymer, one or more in alkylphenol formaldehyde condensation product polyoxyethylene ether,
The dispersant of 0-20% by weight, dispersant is naphthalene or alkyl naphthalene formaldehyde condensation product and salt thereof, fatty alcohol ethylene oxide adduct sulfonate, alkylphenol-polyethenoxy base ether sulfonate, lignin and derivative sulfonate thereof, the alkylaryl sulfonates of polymerization, polycarboxylate, fatty alcohol ethylene oxide adduct phosphate, alkylphenol-polyethenoxy based phosphates, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, polyoxyethylene groups polyoxypropylene base ether block copolymers, alkylphenol-polyethenoxy base ether phosphate, carboxymethyl cellulose, polyvinyl alcohol, polyvinylpyrrolidone, one or more in polyethylene glycol,
Finally mend to 100% with filler.Filler is one or more in kaolin, clay, white carbon, dolomite, diatomite, bentonite, attapulgite, potter's clay, precipitated calcium carbonate.
Processing method is implemented by conventional method.Such as, when processing wetting powder, the fully drying of a certain amount of active ingredient, wetting agent, dispersant and filler, cooling, mixing, air-flow crushing can be milled to required particle diameter and mix, through namely obtaining product after the assay was approved.
Method of application depends on specific environment for use, in all cases, must ensure that their dispersion is trickle as far as possible and even.
When the present composition is used, according to the difference of the factors such as different crops, time of application, weather conditions, be generally 0.5-500 gram of active ingredient/hectare, be preferably 1-100 gram of active ingredient/hectare, generally adopt foliage applying.
Composition pesticide provided by the present invention has the following advantages:
1, Physcion is extraction of substance in plant, and kasugarnycin is microbial metabolic products, and both are very easy to degraded in the environment, to mammal low toxicities such as people and animals, environmentally safe.
2. after Physcion and kasugarnycin mixture, not only maintain the many merits of two kinds of bactericide, and have obvious synergistic effect, the dosage of mixture reduces, and reduces use cost.
3. after Physcion and kasugarnycin mixture, due to different to the mechanism of action of germ, can expanding prevention spectrum, delay the pesticide resistance of germ.
Embodiment
In order to understand essence of the present invention better, be described further by following examples, but the present invention is not limited to these embodiments.
Example of formulations
The preparation of example of formulations 1:2% Physcion+0.5% kasugarnycin aqua, weight percentage, each component proportion is:
The female medicine of 8.5% Physcion is 23.53%,
The former medicine of 74% kasugarnycin is 0.68%,
Wetting agent: fatty alcohol-polyoxyethylene ether 1%,
Cosolvent: 1-METHYLPYRROLIDONE is 3%,
Antifreezing agent: ethylene glycol is 5%,
Preservative: isothiazolinone is 0.5%,
PH conditioning agent: hydrochloric acid is 0.02%,
Finally mend to 100% by deionized water.
By each component through being fully uniformly mixed, filtering and obtaining product.
The preparation of example of formulations 2:0.1% Physcion+10% kasugarnycin wetting powder, weight percentage, each component proportion is:
The female medicine of 8.5% Physcion is 1.18%,
The former medicine of 74% kasugarnycin is 13.51%,
Wetting agent: dialkyl succinylsuccinate sulfonate is 6%,
Filler 1: white carbon is 30%,
Filler 2: kaolin is 49.31%.
Each component drying, cooling, mixing, air-flow crushing are mixed after being milled to reasonable particle diameter again and obtain product.
The preparation of example of formulations 3:1% Physcion+1% kasugarnycin wetting powder, weight percentage, each component proportion is:
The female medicine of 8.5% Physcion is 11.76%,
The former medicine of 74% kasugarnycin is 1.35%,
Wetting agent: fatty alcohol ethylene oxide adduct sulfonate is 1.5%,
Dispersant: lignin derivative sulfonate is 6%,
Filler: kaolin is 79.39%.
Each component drying, cooling, mixing, air-flow crushing are mixed after being milled to reasonable particle diameter again and obtain product.
The preparation of example of formulations 4:0.2% Physcion+6% kasugarnycin suspending agent, weight percentage, each component proportion is:
The female medicine of 8.5% Physcion is 2.35%,
The former medicine of 74% kasugarnycin is 8.11%,
Wetting agent: polyoxyethylene nonylphenol ether is 1%,
Dispersant: alkylphenol-polyethenoxy base ether sulfonate is 3%,
Tackifier: Magnesiumaluminumsilicate is 3%,
Preservative: potassium sorbate 1%,
Antifreezing agent: propane diols 5%,
PH conditioning agent: hydrochloric acid 0.05%,
Finally mend to 100% by deionized water.
Mentioned component is put into high-speed shearing machine be uniformly dispersed, be fully crushed to reasonable particle diameter through sand mill, filter and obtain suspending agent.
The preparation of example of formulations 5:0.5% Physcion+2% kasugarnycin aqua, weight percentage, each component proportion is:
The female medicine of 8.5% Physcion is 5.88%,
The former medicine of 74% kasugarnycin is 2.70%,
Wetting agent: fatty acid amide taurine salt is 2%,
Antifreezing agent: glycerine is 5%,
Preservative: potassium sorbate is 0.5%,
PH conditioning agent: phosphoric acid is 0.12%,
Finally mend to 100% by deionized water.
By each component through being fully uniformly mixed, filtering and obtaining product.
Biological activity determination is tested
BIOLOGICAL ACTIVITY EXAMPLES 1: the indoor biometrics of cucumber anthracnose pathogen is tested
1, for examination germ
Cucumber anthracnose pathogen (Colletotrichumorbiculare): Agricultural University Of South China's plant pathology laboratory provides.
2, condition of culture
In 25 ± 1 DEG C of constant incubators, dark moisturizing is cultivated.
3, instrument and equipment
Electronic balance (sensibility reciprocal 0.1mg), beaker, triangular flask, volumetric flask, graduated cylinder, pipette, culture dish, card punch, high-pressure sterilizing pot, electric heating constant-temperature blowing drying box, superclean bench, multi-functional incubator.
4, test medicine
The female medicine of 8.5% Physcion: Inner Mongolia Kingbo Biotech Co., Ltd.
The former medicine of 74% kasugarnycin: Japanese Hokko Chemical Industry Co., Ltd.;
Other compound proportion medicaments can use with the female medicine of Physcion and the former medicine of kasugarnycin the deionized water dissolving containing proper amount of acetone respectively, and are diluted to required concentration.
5, dosage is arranged
Establish 9 formulas altogether: 8.5% Physcion female medicine, the former medicine of 74% kasugarnycin, 0.1% Physcion+10% kasugarnycin mixture, 0.2% Physcion+6% kasugarnycin mixture, 0.5% Physcion+2% kasugarnycin mixture, 1% Physcion+1% kasugarnycin mixture, 2% Physcion+1% kasugarnycin mixture, 3% Physcion+1% kasugarnycin mixture, 2% Physcion+0.5% kasugarnycin mixture.Female for 5% Physcion medicine, the former medicine of 74% kasugarnycin and 7 different proportion mixtures are made into 80,40,20,10 and 5 μ g/mL drug concentrations respectively.And do blank by the deionized water of equivalent acetone.
6, test repeats: 4 repetitions are all established in every concentration process.
7, test method
Mycelial growth is adopted to suppress method, with reference to " agricultural chemicals indoor biometrics test rule bactericide ".Reagent agent is joined by various dose and melts and be cooled in the quantitative PDA medium of about 50 DEG C, fully pour culture dish into after mixing in.9 agent prescriptions are mixed with the pastille medium of 5 variable concentrations respectively, and 4 repetitions are all established in every concentration process.Treat that medium is cooled to preference temperature, use card punch intercept the germ stem cultivated in the medium, mycelia face down be inoculated in solidified pastille medium central authorities, be placed in dark moisturizing in 25 ± 1 DEG C of constant incubators to cultivate, measure the dull and stereotyped central authorities of PDA for examination, the cucumber anthracnose block cultivated in advance being placed in pastille after 3d, cultivate under constant temperature (27.0 DEG C) non-illuminated conditions.
8, data survey and statistical analysis
Cultivate and measure colony diameter after 3 days, compare with blank, calculate the fungistatic effect of each chemicals treatment to cucumber anthracnose pathogen growth, according to biostatistics probit value conversion table, inhibition percentage is converted into suppression probit value, with the log concentration set in testing as abscissa, suppression probit value is ordinate, calculate the toxicity regression linear equation of medicament, obtain EC 50value.The co-toxicity effect of two kinds of medicaments is passed judgment on co-toxicity coefficient (co-toxicitycoefficient, CTC).Co-toxicity coefficient is less than 80 for antagonism, and being greater than 120 for synergistic effect, is summation action time between 80-120.
Theoretical toxicity index TTI=standard agent toxicity index × standard agent is percentage+reagent agent toxicity index × reagent agent percentage in mixing assembly in mixing assembly
9, interpretation of result and discussion
Under different proportion two kinds of medicament mixtures to the indoor biometrics synergistic results of cucumber anthracnose pathogen in table 1.As can be seen from Table 1, Physcion+kasugarnycin is all greater than 120 with co-toxicity coefficient during 0.1:10,0.2:6,0.5:2,1:1,2:1,3:1 and 2:0.5 proportioning, show that aforementioned proportion compound drug all has obvious synergistic effect, when wherein Physcion and kasugarnycin proportioning are 0.5:2, synergistic effect is the most obvious.
Under table 1 different proportion, Physcion and kasugarnycin are to cucumber anthracnose pathogen indoor biometrics result
A represents Physcion; B represents kasugarnycin
BIOLOGICAL ACTIVITY EXAMPLES 2: the indoor biometrics of Pyricularia oryzae is tested
1, for examination germ
The pathogenic bacteria (PyriculariaoryzaeCav) of rice blast: on the susceptible paddy rice on Agricultural University Of South China farm, be separated and cultivate, fungi preservation is in refrigerator.
2, test medicine
The female medicine of 8.5% Physcion: Inner Mongolia Kingbo Biotech Co., Ltd.
The former medicine of 74% kasugarnycin: Japanese Hokko Chemical Industry Co., Ltd..
3, test method
Adopt mycelial growth rate method, with reference to " agricultural chemicals indoor biometrics test rule bactericide ".Get the sterilizing culture dish of 9cm, pour 20mLPDA medium into, access Pyricularia oryzae bacterial classification after condensation in culture dish central authorities, cultivate under being placed in 25 DEG C of constant temperatures, for subsequent use when mycelia covers with culture dish edge.Reagent agent sterile water is mixed with desired concn, add in sterilizing PDA medium and mix, make the toxic medium of series concentration, be down flat plate, after solidifying, in superclean bench, access rice blast fungus bacterium block, bacterium block diameter is 5mm, puts into the central authorities of toxic flat board, one of mycelia is had to face down, often process and repeat for 4 times, be then placed in the incubator of 25 DEG C, adding sterile water is blank.Measure colony diameter by slide calliper rule right-angled intersection method after 1 week, get its mean value.
4, medicament preparation
Establish 9 formulas altogether: 8.5% Physcion female medicine, the former medicine of 74% kasugarnycin, 0.1% Physcion+10% kasugarnycin mixture, 0.2% Physcion+6% kasugarnycin mixture, 0.5% Physcion+2% kasugarnycin mixture, 1% Physcion+1% kasugarnycin mixture, 2% Physcion+1% kasugarnycin mixture, 3% Physcion+1% kasugarnycin mixture, 2% Physcion+0.5% kasugarnycin mixture.Wherein the former medicine of 74% kasugarnycin and 7 different proportion mixtures are made into 20,10,5,2.5 and 1.25 μ g/mL drug concentrations respectively, and the female medicine of 8.5% Physcion is made into 80,40,20,10 and 5 μ g/mL drug concentrations.And do blank by the deionized water of equivalent acetone.
5, data survey and statistical analysis
Cultivate and measure colony diameter after 7 days, compare with blank, calculate the fungistatic effect that each chemicals treatment grows rice Pyricularia grisea, according to biostatistics probit value conversion table, inhibition percentage is converted into suppression probit value, with the log concentration set in testing as abscissa, suppression probit value is ordinate, calculate the toxicity regression linear equation of medicament, obtain EC 50value.The co-toxicity effect of two kinds of medicaments is passed judgment on co-toxicity coefficient (co-toxicitycoefficient, CTC).Co-toxicity coefficient is less than 80 for antagonism, and being greater than 120 for synergistic effect, is summation action time between 80-120.
Theoretical toxicity index TTI=standard agent toxicity index × standard agent is percentage+reagent agent toxicity index × reagent agent percentage in mixing assembly in mixing assembly
6, interpretation of result and discussion
Under different proportion two kinds of medicament mixtures to the synergistic results of the indoor biometrics of Pathogen of Rice Blast Fungus in table 2.As can be seen from Table 2, Physcion+kasugarnycin is all greater than 120 with co-toxicity coefficient during 0.1:10,0.2:6,0.5:2,1:1,2:1,3:1 and 2:0.5 proportioning, show that aforementioned proportion compound drug all has obvious synergistic effect, when wherein Physcion and kasugarnycin proportioning are 0.5:2, synergistic effect is the most obvious.
Under table 2 different proportion, Physcion and kasugarnycin are to Pathogen of Rice Blast Fungus indoor biometrics result
A represents Physcion; B represents kasugarnycin.
BIOLOGICAL ACTIVITY EXAMPLES 3: to the controlling experiment of powdery mildew of cucumber
1, test medicine:
A:0.5% Physcion aqua (Inner Mongolia Kingbo Biotech Co., Ltd.)
B:2% kasugarnycin aqua (Jiangmen Plant Protection Co., Ltd.'s product)
C:0.5% Physcion+2% kasugarnycin aqua (Jiangmen Plant Protection Co., Ltd.'s preproduction)
2, test method:
Test is planted cucumber farm in one kind, Du Ruan town, Jiangmen City of Guangdong Province and is carried out.Cucumber was sowed on March 8th, 2015 in test, about mu plants 3500 posts, and furrow wide 1.7 meters (bag ditch), spacing in the rows 17-20cm.During dispenser, cucumber is in initial bloom stage, and bottom blade has fragmentary powdery mildew to occur, and does not use bactericide before this.If 3 process and 1 clear water contrast, repeat for four times, Gong16Ge community, randomized arrangement.20 square metres, every community.Drug concentration is arranged: 0.5% Physcion aqua and 2% kasugarnycin aqua are all watered preparation spray medicine by the dosage of active ingredient 20 μ g/ml; 0.5% Physcion+2% kasugarnycin aqua is watered preparation spray medicine according to the dosage of active ingredient 12 μ g/ml.Even spraying is carried out, to squirt cucumber blade face and blade back for degree with the hand compressed sprayer of sharp agriculture board HD-400 type.In cucumber their early stage (April 29) dispenser once, every dispenser in 7 days once, the full phase is total to dispenser three times.Liquid consumption 60 liters/mu.
3, investigation and drug effect computational methods
First time dispenser before and third time dispenser after 10 days investigation the state of an illness.Community diagonal 5 sampling, often some investigation 3 strains, investigate whole blade, total number of sheets of record investigation and the sick number of sheets at different levels.Grade scale is as follows:
0 grade: without scab;
1 grade: lesion area accounts for less than 5% of whole leaf area
3 grades: lesion area accounts for the 6-10% of whole leaf area;
5 grades: lesion area accounts for the 11-20% of whole leaf area;
7 grades: lesion area accounts for the 21-50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Drug effect computational methods:
The total number of sheets investigated according to each community and the sick number of sheets at different levels, calculate disease index and preventive effect.
3, result of the test:
As can be seen from Table 3: in three process, 0.5% Physcion+2% kasugarnycin aqua preventive effect is best, after medicine 10 days is 89.90%, apparently higher than high dose spray medicine under 0.5% Physcion aqua 78.62% and 2% kasugarnycin aqua 50.22%, show significant mixed synergies.
Table 3 three kinds of chemical control powdery mildew of cucumber test of pesticide effectiveness results
Note: above numeral is 4 mean values repeated.

Claims (5)

1. a composition pesticide, is characterized in that, composed of the following components by weight: Physcion accounts for 0.1-2% in the composition, kasugarnycin accounts for 0.5-10%, and all the other are auxiliary agent.
2. composition pesticide according to claim 1, is characterized in that, composed of the following components by weight, Physcion accounts for 0.2-1% in the composition, and kasugarnycin accounts for 1-6%, and all the other are auxiliary agent.
3. composition pesticide according to claim 1 and 2, Physcion is the female medicine of its high-load or the preparation of itself.
4. composition pesticide according to claim 1 and 2, is characterized in that in the composition, and described kasugarnycin is former medicine or the preparation of itself, or indicates the compound of active ingredient with kasugamycin hydrochloride hydrate.
5. composition pesticide according to claim 1 and 2, is characterized in that described composition pesticide mixture becomes aqua, suspending agent or wetting powder.
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WO2018157649A1 (en) * 2017-02-28 2018-09-07 江苏辉丰生物农业股份有限公司 Microbicide composition containing emodin derivative and benzisothiazolinone
CN109315400A (en) * 2018-10-23 2019-02-12 江苏辉丰生物农业股份有限公司 A kind of microbicide compositions
CN109329281A (en) * 2018-10-30 2019-02-15 江苏辉丰生物农业股份有限公司 A kind of microbicide compositions containing Physcion and tetramycin
CN109380221A (en) * 2018-10-29 2019-02-26 江苏辉丰生物农业股份有限公司 A kind of microbicide compositions
CN109845736A (en) * 2018-12-06 2019-06-07 江苏辉丰生物农业股份有限公司 Bactericidal composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Physcion
CN115024320A (en) * 2022-05-26 2022-09-09 四川百事东旺生物科技有限公司 Agricultural composition containing physcion and osthole

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