CN110367283A - A kind of complex micro organism fungicide and its application - Google Patents
A kind of complex micro organism fungicide and its application Download PDFInfo
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- CN110367283A CN110367283A CN201910728966.XA CN201910728966A CN110367283A CN 110367283 A CN110367283 A CN 110367283A CN 201910728966 A CN201910728966 A CN 201910728966A CN 110367283 A CN110367283 A CN 110367283A
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- bacillus
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- organism fungicide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention provides a kind of complex micro organism fungicide and its applications, are related to microorganism formulation technical field.Complex micro organism fungicide provided by the invention, including bacillus subtilis microbial agent, bacillus polymyxa-bacillus amyloliquefaciens mix bacterium agent, chitosan oligosaccharide and chitin oligo saccharide；The mass ratio of the bacillus subtilis microbial agent, the volume of bacillus polymyxa-bacillus amyloliquefaciens mix bacterium agent and chitosan oligosaccharide, chitin oligo saccharide is (40~60) mL:(30~40) mL:(3~6) g:(2~8) g；The mix bacterium agent of the bacillus polymyxa-bacillus amyloliquefaciens is prepared by bacillus polymyxa and bacillus amyloliquefaciens co-fermentation.Complex micro organism fungicide provided by the invention, applied to the prevention and treatment of crop rust, the disease resistance of crop can be improved, reduce the disease incidence of rust, the rust that can be fallen ill simultaneously to the prometaphase plays good therapeutic effect, realizes antibacterial, diseases prevention, key strain and volume increase effect.
Description
Technical field
The present invention relates to microorganism formulation technical fields, and in particular to a kind of complex micro organism fungicide and its application.
Background technique
Rust is to endanger the leaf, stem and fruit of plant by the parasitogenic a kind of plant disease of the rest fungus in fungi.Seriously
When rest fungus sorus it is intensively in blocks, if prevented and treated not in time, scab endangers the yield of crop, causes the underproduction, serious plot
Can the underproduction more than half, most serious causes to have no harvest.When occurring serious such as corn rust, it is covered with sorus on blade, causes big
It is withered to measure blade, plant early ageing, hollow kernels leads to the underproduction, and less serious case's underproduction 10%~20%, severe one is up to 30% or more, very
To causing blade to fracture from aggrieved position, complete stool is withered.
Seed to the prevention of crop rust to select disease resistance strong at present, is subject to field management.It is common when disease occurs
Chemical agent, such as 0.2% Baume degrees lime sulfur, 25% trazodone wettable powder, 25% propiconazole (enemy power de-) missible oil,
43% tebuconazole suspension concentrates, 65% Zineb wettable powder, Bravo and carbendazim etc. are repeatedly sprayed treatment.But chemistry
Drug can bring harm to people and animals and environment.Another chemical pesticide utilization rate is not high, and about 80%~90% residual in the environment, is made
The pollution of pairs of environment.Commonly using chemical pesticide, germ also produces drug resistance, leads to the increasing of times for spraying and dosage
Add, increases the pollution to environment and the destruction to ecology, the vicious circle of pesticide abuse is consequently formed.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of complex micro organism fungicide and its applications.It is provided by the invention
Complex micro organism fungicide can be improved the disease resistance of crop applied to the prevention and treatment of crop rust, reduce the disease incidence of rust, together
When the rust that can fall ill to the prometaphase play good therapeutic effect, realize antibacterial, diseases prevention, key strain and volume increase effect.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of complex micro organism fungicides, including bacillus subtilis microbial agent, bacillus polymyxa-Xie Dian
Mix bacterium agent, chitosan oligosaccharide and the chitin oligo saccharide of afnyloliquefaciens；The bacillus subtilis microbial agent, bacillus polymyxa-Xie Dian
The volume and chitosan oligosaccharide of the mix bacterium agent of afnyloliquefaciens, the mass ratio of chitin oligo saccharide are (40~60) mL:(30~40) mL:(3
~6) g:(2~8) g；The mix bacterium agent of the bacillus polymyxa-bacillus amyloliquefaciens is to be conciliate to form sediment by bacillus polymyxa
Afnyloliquefaciens co-fermentation is prepared.
Preferably, the living bacteria count of the bacillus subtilis microbial agent is 3.5 × 109~5 × 109CFU/m L, it is mostly viscous
The living bacteria count of bacillus-bacillus amyloliquefaciens mix bacterium agent is 2 × 109~5 × 109CFU/mL。
Preferably, the molecular weight of the chitosan oligosaccharide is 350~2000Da, and the molecular weight of chitin oligo saccharide is 400~1000Da.
Preferably, the preparation method of the bacillus subtilis microbial agent includes: the seed liquor by bacillus subtilis with 2%
~5% inoculum concentration is inoculated in the bacillus subtilis fermentation liquor body culture medium after sterilizing in 35~40 DEG C of 48~60h of fermentation,
Obtain bacillus subtilis microbial agent.
Preferably, the bacillus subtilis fermentation liquor body culture medium takes water as a solvent, every liter include: glucose 5~
10g, 10~20g of beancake powder, 5~10g of yeast extract, 40~80g of konjac meal, 2~3g of calcium chloride, 3~5g of dipotassium hydrogen phosphate,
0.3~0.5g of 1~2g of potassium dihydrogen phosphate and magnesium sulfate.
Preferably, the preparation method of the mix bacterium agent of the bacillus polymyxa-bacillus amyloliquefaciens includes: that will glue more
The seed liquor mixed liquor of bacillus and bacillus amyloliquefaciens, it is mostly viscous after sterilizing is inoculated in 5%~10% inoculum concentration
In 30~35 DEG C of 36~48h of fermentation in bacillus and bacillus amyloliquefaciens mixed fermentation liquid body culture medium, more viscous buds are obtained
Spore bacillus-bacillus amyloliquefaciens mix bacterium agent.
Preferably, the volume ratio of the bacillus polymyxa seed liquor and the seed liquor of bacillus amyloliquefaciens be (1.5~
2.5): (0.5~1.5).
Preferably, the bacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid body culture medium take water as a solvent,
Every liter includes: 20~40g of corn flour, 30~60g of beancake powder, 10~50g of drake feather Peptides, 4~5g of calcium chloride, dipotassium hydrogen phosphate
0.5~1g of 1.5~2.5g and potassium dihydrogen phosphate.
The present invention also provides application of the complex micro organism fungicide described in above-mentioned technical proposal in prevention and treatment crop rust.
Preferably, the crop includes corn, wheat, pear tree, peanut, sugarcane, soybean, apple and sunflower.
Beneficial effects of the present invention have the advantage that compared with prior art
(1) present invention will have antagonistic properties, and can generate the bacillus subtilis of the metabolite of antibiotic effect, glue more
Composite bacteria agent is made in bacillus, bacillus amyloliquefaciens, while which occurs antagonism to aecidium, antibacterial
Metabolite also acts on aecidium, plays double effects, can rapidly treat the harm of crop caused by aecidium.
(2) it is added to chitosan oligosaccharide and chitin oligo saccharide in complex micro organism fungicide prepared by the present invention, they are small molecule letters
Number substance can enter the resistant gene of stimulation induction crop in crop body itself, the disease resistance of crop itself be improved, in crop seedling
Phase, the disease resistance of entire growth cycle can be improved using complex micro organism fungicide, play good prevention effect.
(3) present invention is added to konjac meal, the master of konjac meal while fermentation of bacillus subtilis breeds thallus
Wanting ingredient is mannosan, while bacillus subtilis decomposes konjac meal, can induce stimulation metabolism branch and generates more
Antibacterial material improves the disease resistance of microbial inoculum.
(4) present invention is added to drake feather while bacillus polymyxa and bacillus amyloliquefaciens mixed fermentation breed
Peptides, molecular weight are 180~2000, and bacillus polymyxa can quickly be utilized with bacillus amyloliquefaciens, form bud
The time of spore shortens, and promotes metabolism and generates more antibacterial material, improves the disease resistance of microbial inoculum.
(5) complex micro organism fungicide of the invention can reach 94% or more to the prevention disease incidence of crop rust；Early period is sent out
For the rust treatment rate of disease up to 88% or more, the cure rate of mid-term rust can reach 83% or more.
Specific embodiment
The present invention provides a kind of complex micro organism fungicides, including bacillus subtilis microbial agent, bacillus polymyxa-Xie Dian
Mix bacterium agent, chitosan oligosaccharide and the chitin oligo saccharide of afnyloliquefaciens；The bacillus subtilis microbial agent, bacillus polymyxa-Xie Dian
The volume and chitosan oligosaccharide of the mix bacterium agent of afnyloliquefaciens, the mass ratio of chitin oligo saccharide are (40~60) mL:(30~40) mL:(3
~6) g:(2~8) g；The mix bacterium agent of the bacillus polymyxa-bacillus amyloliquefaciens is to be conciliate to form sediment by bacillus polymyxa
Afnyloliquefaciens co-fermentation is prepared.
The preparation method of bacillus subtilis microbial agent of the present invention preferably includes: by the seed liquor of bacillus subtilis
It is inoculated in the bacillus subtilis fermentation liquor body culture medium after sterilizing with 2%~5% inoculum concentration in 35~40 DEG C of fermentations 48
~60h, obtains bacillus subtilis microbial agent.In the present invention, the living bacteria count of the bacillus subtilis microbial agent is preferably
3.5×109~5 × 109CFU/mL, more preferably 4 × 109~4.5 × 109CFU/mL。
The present invention is not particularly limited the source of the bacillus subtilis, and preferably purchase is in Chinese industrial microbial bacteria
Kind preservation administrative center.In the present invention, the preparation method of the bacillus subtilis seed liquor is preferably by bacillus subtilis
The slant strains of bacterium are inoculated in the konjac meal bacterium basal liquid medium containing 1%, in 30~35 DEG C, 180~220r/
Min 14~18h of shaking table culture, obtains bacillus subtilis seed liquor.In the present invention, the bacterium basal liquid medium with
Meter is risen, every liter preferably includes: glucose 10g, peptone 10g, yeast extract 5g, NaCl 5g and excess water.
In the present invention, the bacillus subtilis fermentation liquor body culture medium takes water as a solvent, and every liter preferably includes: grape
5~10g of sugar, 10~20g of beancake powder, 5~10g of yeast extract, 40~80g of konjac meal, 2~3g of calcium chloride, dipotassium hydrogen phosphate 3
0.3~0.5g of~5g, 1~2g of potassium dihydrogen phosphate and magnesium sulfate.
The present invention preferably adds konjac meal, the konjac meal is preferably with matter when preparing bacillus subtilis microbial agent
Amount is added in three times than 2:3:5, the 20% of total konjac meal amount is added in preliminary fermentation culture medium, after fermentation 12 hours
The 30% of total amount of adding again, fermentation added remaining 50% after 24 hours.
The preparation method of the mix bacterium agent of bacillus polymyxa-bacillus amyloliquefaciens of the present invention preferably includes: will
The seed liquor mixed liquor of bacillus polymyxa and bacillus amyloliquefaciens, after being inoculated in sterilizing with 5%~10% inoculum concentration
In 30~35 DEG C of 36~48h of fermentation in bacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid body culture medium, obtain more
The mix bacterium agent of viscous bacillus-bacillus amyloliquefaciens.In the present invention, the bacillus polymyxa-solution starch gemma bar
The living bacteria count of the mix bacterium agent of bacterium is preferably 2 × 109~5 × 109CFU/mL, more preferably 3 × 109~4 × 109CFU/
mL。
The present invention is not particularly limited the source of the bacillus polymyxa and bacillus amyloliquefaciens, preferably purchase in
Chinese industrial Microbiological Culture Collection administrative center.In the present invention, the bacillus polymyxa and bacillus amyloliquefaciens kind
The slant strains of bacillus polymyxa, bacillus amyloliquefaciens are preferably inoculated in by the preparation method of sub- liquid mixed liquor respectively to be contained
In the bacterium basal liquid medium of 0.5% drake feather Peptides, respectively in 28~32 DEG C, 200~250r/min shaking table culture
15~18h respectively obtains bacillus polymyxa seed liquor and bacillus amyloliquefaciens seed liquor, by bacillus polymyxa seed liquor
Bacillus polymyxa and bacillus amyloliquefaciens seed liquor mixed liquor are mixed to get with bacillus amyloliquefaciens seed liquor.In this hair
In bright, the bacterium basal liquid medium is taken water as a solvent, and every liter preferably includes: glucose 10g, peptone 10g, yeast leaching
Powder 5g and NaCl 5g.In the present invention, the bacillus polymyxa seed liquor and the seed liquor of bacillus amyloliquefaciens
Volume ratio is preferably (1.5~2.5): (0.5~1.5), more preferably 2:1.
In the present invention, the bacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid body culture medium are molten with water
Agent, every liter includes: 20~40g of corn flour, 30~60g of beancake powder, 10~50g of drake feather Peptides, 4~5g of calcium chloride, phosphoric acid hydrogen
1.5~2.5g of dipotassium, 0.5~1g of potassium dihydrogen phosphate and excess water.
In the present invention, the chitosan oligosaccharide preferred molecular weight is the chitosan oligosaccharide of 350~2000Da；The chitin oligo saccharide is preferred
Molecular weight is the chitin oligo saccharide of 400~1000Da.The present invention is not particularly limited the source of the chitosan oligosaccharide and chitin oligo saccharide,
Using commercial product.
In the present invention, the preparation method of the complex micro organism fungicide is preferably by bacillus subtilis microbial agent and mostly viscous
It after bacillus-bacillus amyloliquefaciens mix bacterium agent mixes, then mixes with chitosan oligosaccharide, chitin oligo saccharide, obtains composite microbial
Object microbial inoculum.The present invention is not particularly limited the mixed method, using conventional mixing method.In the present invention, institute
The living bacteria count for stating complex micro organism fungicide is preferably 6 × 109~10 × 109CFU/mL, more preferably 7 × 109~8 ×
109CFU/mL。
The present invention also provides application of the complex micro organism fungicide described in above-mentioned technical proposal in prevention and treatment crop rust.
The present invention is not particularly limited the type of the crop, preferably includes corn, wheat, pear tree, peanut, sugarcane, soybean, apple
And sunflower.
In the present invention, the application method of the complex micro organism fungicide is preferably in crops seedling stage by composite microbial bacteria
500~800 times of dilution agent spray blade face, spray altogether three times, are spaced 10 days after spraying every time and are sprayed again；In rust
Complex micro organism fungicide dilution sprays blade face for 100~200 times, sprays altogether twice, between spraying every time afterwards by early stage
It sprays again every two days primary；In rust morbidity mid-term, complex micro organism fungicide is sprayed for three days continuous to blade face without dilution.
Below in conjunction with the embodiment in the present invention, the technical solution in the present invention is clearly and completely described.It is aobvious
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1
The preparation method of complex micro organism fungicide:
(1) the bacterium basal liquid medium for preparing the konjac meal containing 10g/L, by bacillus subtilis under aseptic condition
Slant strains inoculation, in 32 DEG C, 200r/min shaking table culture 16h, obtains seed liquor；
The bacterium basal liquid medium for preparing the drake feather Peptides containing 5g/L, by bacillus polymyxa, solution starch gemma
The strain of bacillus is inoculated with respectively, in 30 DEG C, 220r/min shaking table culture 18h, obtains seed liquor；
(2) bacillus subtilis fermentation liquor body culture medium 100L, each component content are as follows: glucose are prepared in fermentor
500g, beancake powder 1000g, yeast extract 500g, konjac meal 800g, CaCl2200g, K2HPO4300g, KH2PO4100g,
MgSO430g, pH 6.0.It is inoculated with bacillus subtilis seed liquor 2L after sterilizing, controls 35 DEG C of fermentation temperature, revolving speed 300r/m；Hair
When ferment 12h, 1.2kg konjac meal is added, when fermenting for 24 hours, adds 2kg konjac meal, total fermentation time 60h is collected all
Tunning, measurement bacillus subtilis viable count are 7.3 × 109CFU/mL；
(3) bacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid culture medium 100L are prepared in fermentor, respectively
Component content are as follows: corn flour 2000g, beancake powder 3000g, drake feather Peptides 1000g, CaCl2400g, KH2PO450g,
K2HPO4150g, pH6.5.The seed culture fluid of two strains is mixed in mass fraction 2:1 ratio first, is inoculated with 5L seed mixture liquid
In fermentation medium, 30 DEG C of fermentation temperature, revolving speed 300r/m are controlled, after the 60h that ferments, collects whole tunnings, measurement two
The total viable count of kind bacillus mixing is 6 × 109CFU/mL；
(4) it by bacillus subtilis microbial agent 60mL, bacillus polymyxa and bacillus amyloliquefaciens mix bacterium agent 30mL, throws
Enter in blending tank, stir and evenly mix, then put into chitosan oligosaccharide 6g, chitin oligo saccharide 8g into blending tank, stirring promotes dissolution, obtains compound
Microbial bacterial agent, total viable count are 6.86 × 109CFU/mL。
Embodiment 2
The preparation method of complex micro organism fungicide:
(1) bacillus subtilis fermentation liquor body culture medium 100L, each component content are as follows: glucose are prepared in fermentor
800g, beancake powder 1500g, yeast extract 750g, konjac meal 1200g, CaCl2250g, K2HPO4400g, KH2PO4140g,
MgSO440g, pH 6.5.It is inoculated with bacillus subtilis seed liquor 5L, controls 37 DEG C of fermentation temperature, revolving speed 400r/m；Ferment 12h
When, 1.8kg konjac meal is added, when fermenting for 24 hours, adds 3kg konjac meal, total fermentation time 60h collects all fermentations and produces
Object, measurement bacillus subtilis viable count are 8.2 × 109CFU/mL；
(2) bacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid culture medium 100L are prepared in fermentor, respectively
Component content are as follows: corn flour 3000g, beancake powder 4500g, drake feather Peptides 3000g, CaCl2450g, KH2PO470g,
K2HPO4200g, pH6.5.The seed culture fluid of two strains is mixed in mass fraction 2:1 ratio first, is inoculated with 10L seed mixture liquid
In fermentation medium, 32 DEG C of fermentation temperature, revolving speed 400r/m are controlled, after the 60h that ferments, collects whole tunnings, measurement two
The total viable count of kind bacillus mixing is 7.5 × 109CFU/mL；
(3) it by bacillus subtilis microbial agent 50mL, bacillus polymyxa and bacillus amyloliquefaciens mix bacterium agent 35mL, throws
Enter in blending tank, stir and evenly mix, then put into chitosan oligosaccharide 3.5g, chitin oligo saccharide 4g into blending tank, stirring promotes dissolution, is answered
Microorganism formulation is closed, total viable count is 7.9 × 109CFU/mL。
Bacillus subtilis seed liquor, bacillus polymyxa-bacillus amyloliquefaciens seed liquor preparation method is the same as implementation
Example 1.
Embodiment 3
The preparation method of complex micro organism fungicide:
(1) bacillus subtilis fermentation liquor body culture medium 100L, each component content are as follows: glucose are prepared in fermentor
1000g, beancake powder 2000g, yeast extract 1000g, konjac meal 1600g, CaCl2300g, K2HPO4500g, KH2PO4200g,
MgSO450g, pH 6.5.It is inoculated with bacillus subtilis seed liquor 5L, controls 37 DEG C of fermentation temperature, revolving speed 400r/m；Ferment 12h
When, 2.4kg konjac meal is added, when fermenting for 24 hours, adds 4kg konjac meal, total fermentation time 60h collects all fermentations and produces
Object, measurement bacillus subtilis viable count are 9.6 × 109CFU/mL；
(2) bacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid culture medium 100L are prepared in fermentor, respectively
Component content are as follows: corn flour 4000g, beancake powder 6000g, drake feather Peptides 5000g, CaCl2500g, KH2PO4100g,
K2HPO4250g, pH6.5.The seed culture fluid of two strains is mixed in mass fraction 2:1 ratio first, is inoculated with 10L seed mixture liquid
In fermentation medium, 32 DEG C of fermentation temperature, revolving speed 400r/m are controlled, after the 60h that ferments, collects whole tunnings, measurement two
The total viable count of kind bacillus mixing is 8.8 × 109CFU/mL；
(3) it by bacillus subtilis microbial agent 40mL, bacillus polymyxa and bacillus amyloliquefaciens mix bacterium agent 40mL, throws
Enter in blending tank, stir and evenly mix, then put into chitosan oligosaccharide 3g, chitin oligo saccharide 3g into blending tank, stirring promotes dissolution, obtains compound
Microorganism formulation, total viable count are 7.3 × 109CFU/mL。
Bacillus subtilis seed liquor, bacillus polymyxa-bacillus amyloliquefaciens seed liquor preparation method is the same as implementation
Example 1.
Embodiment 4
Started using complex micro organism fungicide described in Examples 1 to 3 in wheat tillering stage, dilutes different multiples, it is right
Foliage-spray, interval spray once again, spray in total 3 times for 10 days.It is compared with water.That prevents rust the results are shown in Table 1.
1 complex micro organism fungicide of table sprays the pre- preventive effect to rust in Wheat Seedling compared with compareing (water)
Fruit
As shown in Table 1, using the complex micro organism fungicide of Examples 1 to 3, wheat rust disease incidence is significantly dropped compared with control group
It is low.
Embodiment 5
Started using complex micro organism fungicide described in Examples 1 to 3 in Maize at Seedling Stage, different multiples is diluted, to leaf
Face sprays, and interval sprays once for 10 days again, sprays in total 3 times.It is compared with water.That prevents rust the results are shown in Table 2.
2 complex micro organism fungicide of table sprays the preventive effect to rust in Maize at Seedling Stage compared with compareing (water)
As shown in Table 2, using the complex micro organism fungicide of Examples 1 to 3, corn rust disease incidence is significantly dropped compared with control group
It is low.
Embodiment 6
The different times occurred using the complex micro organism fungicide of Examples 1 to 3 in wheat rust, dilute different multiples
Liquid level is sprayed afterwards, is control with 50% carbendazol wettable powder chemical pesticide, prevention and treatment the results are shown in Table 3.
The control efficiency of 3 complex micro organism fungicide of table and carbendazim to wheat rust
As shown in Table 3, the complex micro organism fungicide of Examples 1 to 3,86% is reached to the control efficiency of wheat rust~
89%.
Embodiment 7
The different times occurred using the complex micro organism fungicide of Examples 1 to 3 in corn rust, dilute different multiples
Liquid level is sprayed afterwards, is control with 50% carbendazol wettable powder chemical pesticide, prevention and treatment the results are shown in Table 4.
The control efficiency of 4 complex micro organism fungicide of table and carbendazim to corn rust
As shown in Table 4, the complex micro organism fungicide of Examples 1 to 3,83% is reached to the control efficiency of corn rust~
86%.
It is become rusty by embodiment 4~7 it is found that preferably can be used to prevent and treat crop using complex micro organism fungicide of the invention
Disease.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of complex micro organism fungicide, which is characterized in that including bacillus subtilis microbial agent, bacillus polymyxa-solution starch
Mix bacterium agent, chitosan oligosaccharide and the chitin oligo saccharide of bacillus；The bacillus subtilis microbial agent, bacillus polymyxa-solution starch
The volume and chitosan oligosaccharide of the mix bacterium agent of bacillus, the mass ratio of chitin oligo saccharide are (40~60) mL:(30~40) mL:(3~
6) g:(2~8) g；The mix bacterium agent of the bacillus polymyxa-bacillus amyloliquefaciens is to conciliate starch by bacillus polymyxa
Bacillus co-fermentation is prepared.
2. complex micro organism fungicide according to claim 1, which is characterized in that the bacillus subtilis microbial agent it is effective
Viable count is 3.5 × 109~5 × 109CFU/mL, effective viable bacteria of bacillus polymyxa-bacillus amyloliquefaciens mix bacterium agent
Number is 2 × 109~5 × 109CFU/mL。
3. complex micro organism fungicide according to claim 1, which is characterized in that the molecular weight of the chitosan oligosaccharide be 350~
2000Da；The molecular weight of the chitin oligo saccharide is 400~1000Da.
4. complex micro organism fungicide according to claim 1, which is characterized in that the preparation of the bacillus subtilis microbial agent
Method includes: that the seed liquor of bacillus subtilis is inoculated in the bacillus subtilis hair after sterilizing with 2%~5% inoculum concentration
In 35~40 DEG C of 48~60h of fermentation in ferment fluid nutrient medium, bacillus subtilis microbial agent is obtained.
5. complex micro organism fungicide according to claim 4, which is characterized in that the bacillus subtilis fermentation liquor body training
Feeding base takes water as a solvent, and every liter includes: 5~10g of glucose, 10~20g of beancake powder, 5~10g of yeast extract, konjac meal 40
~80g, 2~3g of calcium chloride, 0.3~0.5g of 3~5g of dipotassium hydrogen phosphate, 1~2g of potassium dihydrogen phosphate and magnesium sulfate.
6. complex micro organism fungicide according to claim 1, which is characterized in that the bacillus polymyxa-solution starch bud
The preparation method of the mix bacterium agent of spore bacillus includes: the seed liquor mixed liquor by bacillus polymyxa and bacillus amyloliquefaciens,
Bacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid body culture after sterilizing are inoculated in 5%~10% inoculum concentration
In 30~35 DEG C of 36~48h of fermentation in base, bacillus polymyxa-bacillus amyloliquefaciens mix bacterium agent is obtained.
7. complex micro organism fungicide according to claim 6, which is characterized in that the bacillus polymyxa seed liquor reconciliation
The volume ratio of the seed liquor of bacillus amyloliquefaciens is (1.5~2.5): (0.5~1.5).
8. complex micro organism fungicide according to claim 6, which is characterized in that the bacillus polymyxa conciliates starch bud
Spore bacillus mixed fermentation liquid culture medium takes water as a solvent, and every liter includes: 20~40g of corn flour, 30~60g of beancake powder, drake feather
10~50g of Peptides, 0.5~1g of 4~5g of calcium chloride, 1.5~2.5g of dipotassium hydrogen phosphate and potassium dihydrogen phosphate.
9. application of the complex micro organism fungicide described in claim 1~8 any one in prevention and treatment crop rust.
10. application according to claim 9, which is characterized in that the crop includes corn, wheat, pear tree, peanut, sweet
Sugarcane, soybean, apple and sunflower.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110692480A (en) * | 2019-11-11 | 2020-01-17 | 广西壮族自治区农业科学院 | Method for increasing sugarcane yield and prolonging perennial root life by obliquely cutting and reseeding sugarcane |
| CN112493255A (en) * | 2020-12-02 | 2021-03-16 | 青岛博智汇力生物科技有限公司 | Plant growth regulator containing chitosan oligosaccharide |
| CN112573958A (en) * | 2020-12-14 | 2021-03-30 | 李桂丽 | Microbial agent with disease and pest control function and preparation method thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1480522A1 (en) * | 2002-02-26 | 2004-12-01 | Winston Company, Inc. | Aligicidal fermentated straw / barley matrix |
| CN103300069A (en) * | 2013-05-22 | 2013-09-18 | 江苏省绿盾植保农药实验有限公司 | Biological bactericide composition and application thereof to wilt |
| CN103651582A (en) * | 2013-09-17 | 2014-03-26 | 周剑平 | Broad-spectrum composite microbial fungicide and preparation method thereof |
| CN103865843A (en) * | 2014-02-13 | 2014-06-18 | 河北省科学院生物研究所 | Compound microbial bacteria and application thereof in prevention and treatment of vegetable fungal diseases |
| CN105754608A (en) * | 2016-03-28 | 2016-07-13 | 鹤壁市人元生物技术发展有限公司 | Microbial soil conditioner and preparation method thereof |
| CN106417386A (en) * | 2016-09-22 | 2017-02-22 | 黑龙江田力保生物科技有限公司 | Leaf type wettable biological agent and use method thereof |
| CN107226730A (en) * | 2017-05-22 | 2017-10-03 | 咸阳润源生物科技有限公司 | A kind of preparation method of biological organic fertilizer |
| CN107382622A (en) * | 2017-08-17 | 2017-11-24 | 山东天沃生物工程有限公司 | A kind of microbial bacterial agent and its application containing Paenibacillus polymyxa |
| CN108220199A (en) * | 2018-02-06 | 2018-06-29 | 浦江县欧立生物技术有限公司 | A kind of preparation method of complex microbial inoculum |
| CN108753664A (en) * | 2018-06-28 | 2018-11-06 | 广东省农业科学院农业资源与环境研究所 | A kind of biocontrol agent, biological organic fertilizer and its application |
| CN109136144A (en) * | 2018-09-11 | 2019-01-04 | 通化医药高新区恩维泽生物科技有限公司 | A kind of ginseng special composite biological microbial inoculum and production preparation method |
-
2019
- 2019-08-08 CN CN201910728966.XA patent/CN110367283B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1480522A1 (en) * | 2002-02-26 | 2004-12-01 | Winston Company, Inc. | Aligicidal fermentated straw / barley matrix |
| CN103300069A (en) * | 2013-05-22 | 2013-09-18 | 江苏省绿盾植保农药实验有限公司 | Biological bactericide composition and application thereof to wilt |
| CN103651582A (en) * | 2013-09-17 | 2014-03-26 | 周剑平 | Broad-spectrum composite microbial fungicide and preparation method thereof |
| CN103865843A (en) * | 2014-02-13 | 2014-06-18 | 河北省科学院生物研究所 | Compound microbial bacteria and application thereof in prevention and treatment of vegetable fungal diseases |
| CN105754608A (en) * | 2016-03-28 | 2016-07-13 | 鹤壁市人元生物技术发展有限公司 | Microbial soil conditioner and preparation method thereof |
| CN106417386A (en) * | 2016-09-22 | 2017-02-22 | 黑龙江田力保生物科技有限公司 | Leaf type wettable biological agent and use method thereof |
| CN107226730A (en) * | 2017-05-22 | 2017-10-03 | 咸阳润源生物科技有限公司 | A kind of preparation method of biological organic fertilizer |
| CN107382622A (en) * | 2017-08-17 | 2017-11-24 | 山东天沃生物工程有限公司 | A kind of microbial bacterial agent and its application containing Paenibacillus polymyxa |
| CN108220199A (en) * | 2018-02-06 | 2018-06-29 | 浦江县欧立生物技术有限公司 | A kind of preparation method of complex microbial inoculum |
| CN108753664A (en) * | 2018-06-28 | 2018-11-06 | 广东省农业科学院农业资源与环境研究所 | A kind of biocontrol agent, biological organic fertilizer and its application |
| CN109136144A (en) * | 2018-09-11 | 2019-01-04 | 通化医药高新区恩维泽生物科技有限公司 | A kind of ginseng special composite biological microbial inoculum and production preparation method |
Non-Patent Citations (3)
| Title |
|---|
| 丁新天 主编: "《县域植保推广技术创新与实践》", 31 July 2014, 光明日报出版社 * |
| 中国药学会生化药物专业委员会 等举办: "《海洋天然产物与天然生化药物论文荟萃-全国第二届海洋生命活性物质与天然生化药物学术讨论会论文集》", 31 December 1998, 海军医学研究所情报中心 * |
| 尤民生 主编: "《提高农业综合生产能力的理论与实践》", 30 September 2005, 海风出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110692480A (en) * | 2019-11-11 | 2020-01-17 | 广西壮族自治区农业科学院 | Method for increasing sugarcane yield and prolonging perennial root life by obliquely cutting and reseeding sugarcane |
| CN112493255A (en) * | 2020-12-02 | 2021-03-16 | 青岛博智汇力生物科技有限公司 | Plant growth regulator containing chitosan oligosaccharide |
| CN112573958A (en) * | 2020-12-14 | 2021-03-30 | 李桂丽 | Microbial agent with disease and pest control function and preparation method thereof |
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