CN1930991A - Re-compounded germicide containing Bucillus licheniformis - Google Patents
Re-compounded germicide containing Bucillus licheniformis Download PDFInfo
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Abstract
The re-compounded germicide containing Bacillus liceniformis is re-compounded with Bacillus liceniformis and one or two of the biological germicides or chemical germicides in certain weight proportion. The biological germicides or chemical germicides include validamycin A, Zhongshengmycin, polyoxin, ningnanmycin, kasugamycin, etc. The re-compounded germicide containing Bacillus liceniformis may be used in killing germs of Ascomycetae, Rhizopus, Fusarium, etc. and possesses high efficiency.
Description
Technical field
The invention belongs to the composite bactericide that contains bacillus licheniformis of composite bactericide, particularly one class preventing and control corps diseases.
Background technology
Bacillus licheniformis (Bacillus licheniformis) is the amphimicrobian saprophytic bacteria that a class natural world extensively exists.This bacterium is always as industrial production bacterium such as proteolytic enzyme, amylase, bioactivator and probios, for animals and medical aspect be used widely, very low to health and environmental toxicity.Bacillus licheniformis is also existing many researchs aspect the cultural control disease, wherein many plant pathogenic fungis, pathogenic Gram-negative bacteria are had antagonism.As in " use in the field of antagonistic bacterium GP13 control black shank " (Yunnan Prov Agriculture University's journal 2003.1 side Dunhuang), reported that bacillus licheniformis GP13 reaches 40.1%～90.5% to the black shank control efficiency.
Bacillus licheniformis significant feature mechanism: the 1. competition effect of dominant population---bacillus licheniformis has the advantages that colonization ability is strong on crop plant, breeding is fast, can infect the site with the pathogen effective competition, reduces the plant disease morbidity risk rate.2. the plain antibiosis of antagonism---bacillus licheniformis can secrete generation antagonism element, can suppress effectively the pathogenic sclerotium growth, infect and spread.3. the induction of resistance effect of antagonism bacterium---bacillus licheniformis can be induced a large amount of generations enzyme relevant with disease resistance in the crop plant behind foliage-spray, resist the ability that pathogen is invaded and spreads thereby strengthened crop.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of control efficiency to disease, composite bactericide that contains bacillus licheniformis that active total amount is used in reduction of strengthening.
The present invention solves the problems of the technologies described above with following technical scheme: bacillus licheniformis and a kind of of biological bactericide or chemical bactericide or two kinds are mixed into regular dosage form by a certain percentage use.
Described biological bactericide or chemical bactericide are a kind of in jinggangmeisu, middle living rhzomorph, polyoxin, Ningnanmycin, kasugarnycin, Trichoderma, Propamocarb, dimethomorph, carbendazim, tmtd, hymexazo, fenaminosulf, cytosintetidemycin, doxycycline monohydrate, amino-oligosaccharide, Fluoxastrobin, antibiotic in pyrimidine nucleoside class (pesticide corrosion 120), Paenibacillus polymyxa, Pseudomonas fluorescence, Paecilomyces lilacinus bacterium, bacillus subtilis, the Bacilluscereus, or two kinds.
Living spores number 〉=8 * 10 that bactericide bacillus licheniformis of the present invention contains
7Individual/(gram or ml).Composite composition is 0.5～95.0% by weight of formulation than jinggangmeisu, in mildew plain 0.5～99.9%, polyoxin is 0.1～95.0%, Ningnanmycin is 0.5～95.0%, kasugarnycin 0.5～95.0%, Propamocarb 0.5～95.0%, dimethomorph 0.5～95.0%, carbendazim 0.5～95.0%, tmtd 0.5～99.9% ， hymexazo 0.5～95.0%, fenaminosulf 0.5～95.0%, cytosintetidemycin 0.5～95.0%, doxycycline monohydrate 0.5～95.0%, amino-oligosaccharide 0.5～95.0%, Fluoxastrobin 0.5～95.0%, antibiotic in pyrimidine nucleoside class (pesticide corrosion 120) 0.5～95.0%, bacillus subtilis living spores number 〉=5 * 10
7Individual/(gram or ml), Paenibacillus polymyxa 5 * 10
6Individual/(gram or ml), Pseudomonas fluorescence 5 * 10
6Individual/(gram or ml), waxy Bacillus living spores number 〉=5 * 10
7Individual/(gram or ml), Trichoderma 〉=5 * 10
7Individual/(gram or ml).
With the composite bactericide that contains bacillus licheniformis of the present invention, be used to prevent and treat Rhynchosporium spp, Saccharomyces, nuclear cavity Pseudomonas, rhizopus, Fusarium, Phytophthora and peronospora, effective especially, its reason is:
1, according to the microecology principle, by introducing microbial bacterial agent, the reasonable distribution that handle management plant root leaf encloses microbiologic population in the ecotope, worsen the living environment of pathogen, in conjunction with cultivation management, agricultural and chemical control measure, form stable a, balance, the bio-diversity ecosystem, thereby effectively and more enduringly the controlling plant disease is popular.
2, utilize antibiotic and chemical bactericide that the plant mycelial growth is had good killing action, the inhibitory action that cooperates bacillus licheniformis that the plant disease sclerotium is sprouted, after composite, form the synergistic effect of having complementary advantages, thereby reach the purpose of sustainable Control disease.
3, can carry out composite with arbitrary proportion former medicine of bacillus licheniformis and above-mentioned various material, as long as choose appropriate compound proportion, the application of agricultural chemicals of the present invention is reached to bring into play the optimum medicine efficacy effect, can reduce the purpose of antibiotic usage amount and access times again.
4, can utilize the characteristic of bacillus licheniformis to the height tolerance of bad condition; After measured, there is not harmful effect in the gemma formation to bacillus licheniformis behind above-mentioned each composite reagent.
Embodiment
The biological bactericide or the chemical bactericide that can be mixed with bacillus licheniformis are following material:
Jinggangmeisu: be the water soluble antibiotics-glucoside compounds that produces by the mutation of water streptomycete well ridge.Have 6 components, main active substances is jinggangmycin A and B.Pure product are the colorless and odorless hygroscopic powder, and fusing point 130-135 ℃ (decomposition) disregarded under the vapour pressure room temperature, can be very fast water-soluble.Jinggangmeisu is the very strong farm antibiotics of interior absorption; have protection and treatment double action; after it contacts the mycelia of rice sheath blight disease germ; can be absorbed very soon and in thalline, conduct by somatic cells; disturb and suppress the growth of somatic cells normal growth to make germ lose the infringement ability, thereby play therapeutic action.
The middle rhzomorph of giving birth to: chemical name is the amino L-lysine-2 of a 1-N glucoside base streptolidine base-2-deoxidation gulose amine.Former medicine is a buff powder, and fusing point 173-190 ℃, 100% is water-soluble.Middle living rhzomorph is a N-glucoside antibiotic, and its has a broad antifungal spectrum can resist mycobacterium, saccharomycete and filamentous fungi.Especially crops pathogenic bacteria such as dish Bacteria erwinia, angular leaf spot of cucumber bacterium, Xanthomonas oryzae, fusarium graminearum etc. all had tangible antibacterial activity.
Polyoxin: have another name called the peptide pyrimidine nucleoside, molecular formula is C
17H
25N
5O
14, former medicine is an amorphous powder, fusing point＞160 ℃ (decomposition), solvability 1kg/L (20 ℃ of water), acetone, methyl alcohol and organic solvent＜100mg/L commonly used, the moisture absorption belongs to the broad-spectrum antibiotics class, has the interior preferably conduction of inhaling; Its mechanism of action is to disturb the chitinous biosynthesis of bacterial cell wall, and germ tube is with after mycelium contact medicament, and expanding, break, overflow cell inclusion because of the part can not normal development, causes death, suppresses germ product spore and scab expansion effect in addition; This medicine does not have toxicity to animal, and plant is not had poisoning.Main controlling object has wheat powdery mildew, the red disease of tobacco, cucumber downy mildew, cucurbits fusarium wilt, genseng black spot, rice sheath blight disease, alternaria leaf spot of apple, strawberry and multiple fungal diseases such as worker's grape grey mould, forest spike top and pears black spot.
Ningnanmycin: chemical name is 1-(4-flesh glutamine-L-silk amide-4-deoxidation-β-D-glucopyranose aldehyde acid amides) cytimidine, molecular formula C
16H
23O
8N
7, be a kind of cytidine peptide type broad-spectrum antibiotic bactericide, have prevention, therapeutic action.Tobacco mosaic virus disease there is good control efficiency, has characteristics such as anti-rain drop erosion, toxicity be low.
Kasugarnycin: chemical name is [5-amino-2-methyl-6-(2,3,4,5,6-carbonyl cyclohexyl oxo) pyrans-3-base amino-a-IMDA], molecular formula C
14H
25N
3O
9, be agricultural antibiotic, have stronger interior absorption, this medicine mainly disturbs amino acid whose metabolism esterase system, thereby influences the synthetic of protein, suppresses the mycelia elongation and causes cell granulationsization, but spore germination is not had influence.Kasugarnycin is the special-purpose antibiotic medicament of rice blast, tool prevention and therapeutic action.
Trichoderma (trichoderma SP): have another name called Trichoderma, sterilization ling, Te Like, trichodermin, the platymiscium source bactericide, the biochemical mechanism of its biological and ecological methods to prevent plant disease, pests, and erosion effect mainly comprises: metabolism chitinase, β-1, multiple extracellular enzymes such as 3-dextranase, protease, the cell wall of intense hydrolysis plant pathogenic fungi, or make fungal cell wall discharge inducer (elicitor), start the defense reaction of plant; Metabolism produces antibiotic class materials such as pentanone, octanone, terpene, amino acid derivativges, trichodermin, gliotoxin, viridin, antibacterial peptide; Produce the inducible factor of plant defense reaction, inducing plant resistance; Strengthen plant tissue peroxidase, superoxide dismutase and catalase, polyphenol oxidase activity, diaphragm fat is not oxidized; Toxigenicity albumen, or, suppress the pathogen growth and breeding with the number of mechanisms joint action of antagonism.Gray mold, downy mildew to plant have favorable effect.
Propamocarb: chemical name 3-(dimethylamino) propyl carbamic acid propyl ester, the curative fungicide agent of inhaling conductive performance in having is a kind of new and effective, systemic fungicide.Control crops, particularly vegetables, the downy mildew of fruit tree, eqpidemic disease, damping off there is excellent control efficiency, can stop germ to produce and the formation spore, can not only enter root control root disease rapidly in the plant root processing, also can prevent and treat the disease of ground plant.
Dimethomorph: have another name called An Ke-MnZn, chemical name be (E, Z)-4-[3-(4-chlorphenyl) 3-(3, the 4-Dimethoxyphenyl) acryloyl] morpholine, molecular formula C
21H
22C
1NO
4Be the single-minded Oomycete fungi bactericide that kills, its effect characteristics are to destroy the formation of cell envelope, and all there is effect in each stage of oomycetes history of life, the formation stage at sporangiophore and egg spore is particularly responsive, and (＜0.25 μ g/ml) promptly is suppressed under extremely low concentration.There is not cross resistance with the phenylamide medicament.
Carbendazim: chemical name benzimidazolyl-2 radicals-aminocarbamic acid methyl esters, high-efficiency low-toxicity, effective to sac fungi and imperfect fungus, invalid to oomycetes and bacterial disease, have protection and therapeutic action.The mechanism of action is the formation of imitative hammer body in the mitosis of disturbing bacterium, thereby influences cell division.
Tmtd: have another name called tetramethyl thiuram disulfide, molecular formula C
6H
12N
2S
4, be a kind of bactericide of tool protective effect, its has a broad antifungal spectrum is mainly used in and handles seed and soil, the seedling blight of control cereal class smut and various crop.Also can be used for spraying, prevent and treat some fruit trees, vegetable disease.
Hymexazo: chemical name is a 3-hydroxy-5-methyl base isoxazole, and molecular formula is C
4H
5NO
2, systemic fungicide is again a kind of soil disinfectant simultaneously, the damping off that rotten mildew, sickle-like bacteria etc. are caused has preventive effect preferably.This medicine combines with iron, aluminium ion in the soil, suppresses the germination of spore.Hymexazo can move by the root absorption of plant and in root system, produces two kinds of glucosides at the plant intracellular metabolite, and crop is had the effect that improves physiologically active, thereby can promote the branch evil of plant strain growth, root, the increase of root hair and the activity of root to improve.
Bacillus subtilis: belong to biological bactericide, bacterial classification obtains brevibacterium from soil or axis separation.Be exclusively used in the Cotton seeds rice paddy seed, have the effect that activates plant growth, alleviates germ harm such as bacterial leaf streak of rice, bacterial leaf-blight, bakanae disease.The mode of occupying living space by competitive growth and breeding stops the growth of plant pathogenic fungi, can form the highly dense diaphragm of one deck rapidly at plant surface, makes phytopathogen can not get living space, thereby has protected crops to avoid pathogen harm.Bacillus subtilis can secrete antibacterial substance, suppresses germ spore-germination and mycelial growth, thereby reaches prevention and the purpose for the treatment of.
Waxy Bacillus (bacillus cereus): with the single sporeformer mix preparation of vacation outward appearance be faint yellow or the light brown emulsion form, slightly toughness has special fishy smell.Density 1.08 gram/cubic centimetres, pH6.5-8.4 is stable below 45 ℃.Belong to the biogenic bactericide, significant feature mechanism is to produce a large amount of antagonist proteins rice sheath blight disease is had preventive effect preferably.Simultaneously various vegetables soil-borne diseases such as cucumber fusarium axysporum also there is stronger antagonistic ability.
Fenaminosulf: have another name called fenaminosulf, Dexon, the yellowish-brown powder, about 200 ℃ of decomposition, water soluble 20-30g/L (25 ℃), dimethyl formamide, ethanol etc. are seed and processing soil treatment agent, inhale osmosis in the tool.Root disease to vegetables has good preventive effect.
Cytosintetidemycin: chemical name: cytidine peptide, outward appearance are stable brown homogeneous liquid, do not have visible suspension and sediment.195 ℃ of fusing points are stablized light, heat, acid, in the alkaline state instability.Function with the effect of propagation such as the plant viruses that suppress vegetables, melon and fruit, crop and adjusting, promotion growth and development of plants.
Amino-oligosaccharide: oligosaccharins, chemical name: oligomeric-the D Glucosamine.A kind of bactericide that this medicine microorganism belonging to genus metabolism is extracted with resistant effect, some pathogen growth there is inhibitory action, as influence fungus spore germination, bringing out the mycelia form morphs, biochemical reaction changes etc. in the born of the same parents of mycelia, the mechanism that inducing plant produces disease resistance mainly is to excite gene expression in plants, produces chitinase, dextranase, guarantor's element and PR albumen etc. with antibacterial action; Have the gene expression that suppresses germ simultaneously, the Physiology and biochemistry of mycelia is morphed, growth is suppressed, simultaneously can also stimulating growth.
Doxycycline monohydrate: chemical name: 6-methyl-4-(dimethylamino)-3,5,6,10,12,12a-hexahydroxy-1,11-dioxo-1,4,4a, 5,5a, 6,11,12a-octahydro-2-aphthacene carboxamide hydrochloride,
The effect characteristics: the synthetic and messenger ribonucleic acid that disturbs bacterioprotein combine with 30S ribosomal subunit and suppresses the prolongation of peptide chain, and Gram-negative bacteria and positive bacteria etc. is all had than inhibitory action by force.The control cucumber bacterial angular leaf spot has the better prevention effect.Can use with antibiotic agricultural chemicals, organophosphorous pesticide.
Paenibacillus polymyxa: have another name called health ground flower bud moral, khaki particulate, relative proportion 0.42, active ingredient can be dissolved in water.This medicine belongs to microbial pesticide, and the vegetative bacteria bacterial wilt is had good preventive effect.
Pseudomonas fluorescence (pseudomonas fluorescens): the preparation outward appearance is the grey powder, pH6.0～7.5.This product is protective plant such as to capture by the nutrient competition of PFI antagonistic bacterium, site to avoid infecting of pathogen, this product be mainly used in plant-bacterial-wilts such as tomato, tobacco control and can vernalization strong sprout, impel plant growing to have the double action of diseases prevention and bacterial manure.
Fluoxastrobin: have another name called the Amici and reach, peace is gone out and is reached, (E)-2-{2-[6 (2-cyano-benzene oxygen) pyrimidine-4-base oxygen] phenyl }-the 3-methoxy propyl, the mitochondrial respiratory inhibitor promptly shifts by electronics between cytochrome B and C and suppresses mitochondrial breathing.The bacterial strain that 14-demethylation enzyme inhibitor, benzamides, dicarboxylic dihydrazides amine and benzimidazole is produced resistance is effective.Have protection, treat, root out, infiltration, systemic activity.
Antibiotic in pyrimidine nucleoside class: have another name called pesticide corrosion 120, antimycoin 120,120 agricultural antibiotics.Former medicine outward appearance is a white powder, and fusing point 165-167 ℃ (decomposition) is soluble in water, is insoluble to organic solvent, and is stable in acid and neutral medium, unstable in the alkaline medium.Be broad spectrum antibiotic, it has had strong inhibitory effects to many phytopathogens, and is better to cucurbits powdery mildew, flowers powdery mildew and wheat rust preventive effect.
Composite bactericide of the present invention contains bacillus licheniformis living spores number 〉=8 * 10
7Individual/(gram or ml) is mixed into the regular dosage form use by a certain percentage with bacillus licheniformis and a kind of of following biological bactericide or chemical bactericide or two kinds during preparation.
Composite composition is 0.5～95.0% by weight of formulation than jinggangmeisu, in the element that mildews be 0.5～99.9%, polyoxin is 0.1～95.0%, Ningnanmycin is 0.5～95.0%, kasugarnycin 0.5～95.0%, Propamocarb 0.5～95.0%, dimethomorph 0.5～95.0%, carbendazim 0.5～95.0%, tmtd 0.5～99.9% ， hymexazo 0.5～95.0%, fenaminosulf 0.5～95.0%, cytosintetidemycin 0.5～95.0%, doxycycline monohydrate 0.5～95.0%, amino-oligosaccharide 0.5～95.0%, Fluoxastrobin 0.5～95.0%, antibiotic in pyrimidine nucleoside class (pesticide corrosion 120) 0.5～95.0%, bacillus subtilis living spores number 〉=5 * 10
7Individual/(gram or ml), Paenibacillus polymyxa 5 * 10
6Individual/(gram or ml), Pseudomonas fluorescence 5 * 10
6Individual/(gram or ml), waxy Bacillus living spores number 〉=5 * 10
7Individual/(gram or ml), or Trichoderma 〉=5 * 10
7Individual/(gram or ml).
In compound process, lichens gemma bar content is high more in as the former medicine of the lichen bacillus ferments of host, and shared part by weight just can be high more in finished medicines for the bactericide composite with it.In theory, the order of magnitude scope that contains bacillus licheniformis in the former medicine of the lichen bacillus ferments can be very big, so shared weight ratio scope also can be very big in finished medicines for bactericide.
Described preparation be routinely the preparation method, promptly in composite composition, add known solvent or carrier, emulsifier and dispersant and make required formulation.
Embodiment 1
Take by weighing former powder 15.8 grams of 95% jinggangmeisu, add in 16,000,000,000 living spores/ml the lichen bacillus ferments liquid 250 grams, and adding emulsifier 0203B 25 grams, remaining water is supplied 500 grams, fully makes 8,000,000,000/ml of 3% jinggangmeisu bacillus licheniformis aqua, 500 grams behind the mixed dissolution.
Embodiment 2
Claim 32% polyoxin former powder 46.8 grams, 16,000,000,000/ml the lichen bacillus ferments stoste, 250 grams, emulsifier 0203B25 gram and water 178.2 grams.Earlier the former powder of polyoxin is added in the lichen bacillus ferments stoste, the limit edged stirs, add 0203B after the dissolving while stirring, after adding water after again and supplying 500 grams and stir, promptly make 8,000,000,000 living spores of 3% polyoxin/ml bacillus licheniformis aqua 500 grams.
Embodiment 3
Claim to give birth in 90% rhzomorph former powder 5.6 grams, 16,000,000,000/ml the lichen bacillus ferments stoste, 250 grams, emulsifier 0203B25 gram and water 219.4 grams.Earlier the former powder of middle living rhzomorph is added in the lichen bacillus ferments stoste, the limit edged stirs, add 0203B after the dissolving while stirring, after adding water after again and supplying 500 grams and stir, promptly make and give birth to 8,000,000,000 living spores of rhzomorph/ml bacillus licheniformis aqua 500 grams in 1%.
Embodiment 4
Claim 96% hymexazo former powder 26 grams, 16,000,000,000/ml the lichen bacillus ferments stoste, 250 grams, emulsifier 0203B 25 grams and water 199 grams.Earlier the former powder of hymexazo is added in the lichen bacillus ferments stoste, the limit edged stirs, and adds 0203B after the dissolving while stirring, after adding water after again and supplying 500 grams and stir, promptly makes 8,000,000,000 living spores of 5% hymexazo/ml bacillus licheniformis aqua 500 grams.
Embodiment 5
Claim 80% Propamocarb stoste 93.8 grams, 16,000,000,000/ml the lichen bacillus ferments stoste, 250 grams, emulsifier 0203B25 gram and water 131.2 grams.Earlier Propamocarb stoste is added in the lichen bacillus ferments stoste, the limit edged stirs, and adds the 0203B_25 gram then, after adding water after again and supplying 500 grams and stir, promptly makes 8,000,000,000 living spores of 15% Propamocarb/ml bacillus licheniformis aqua 500 grams.
Embodiment 6
With the former powder of bacillus licheniformis (30,000,000,000/gram) 100 grams, the former powder of bacillus subtilis (30,000,000,000/gram) 100 grams, draw back powder 20 grams, dispersing agent NNO _ 50 grams, precipitated calcium carbonate 230 grams, after mixing the back grinding, obtain 6,000,000,000/gram of 6,000,000,000/gram bacillus licheniformis bacillus subtilis wetting powder, 500 grams.
Embodiment 7
With the former powder of bacillus licheniformis (30,000,000,000/gram) 100 grams, former powder 96%31.3 grams of dimethomorph draw back powder 70 grams, dispersing agent NNO _ 50 grams, precipitated calcium carbonate 248.7 grams after grinding after mixing, obtain 6,000,000,000 of 6% dimethomorphs/gram bacillus licheniformis wetting powder 500 grams.
Embodiment 8
With the former powder of bacillus licheniformis (30,000,000,000/gram) 100 grams, 98% carbendazim original powder, 76.5 grams draw back powder 50 grams, dispersing agent NNO 50 grams, precipitated calcium carbonate 223.5 grams after grinding after mixing, obtain 6,000,000,000/gram of 500 grams, 15% carbendazim bacillus licheniformis wetting powder.
Embodiment 9
With the former powder of bacillus licheniformis (30,000,000,000/gram) 100 grams, former powder 79 grams of 95% tmtd draw back powder 50 grams, dispersing agent NNO 50 grams, precipitated calcium carbonate 221 grams after grinding after mixing, obtain 6,000,000,000/gram of 500 grams, 15% tmtd bacillus licheniformis wetting powder.
Embodiment 10
With the former powder of bacillus licheniformis (30,000,000,000/gram) 100 grams, former powder 15 grams of 65% kasugarnycin draw back powder 50 grams, dispersing agent NNO 50 grams, precipitated calcium carbonate 300 grams after grinding after mixing, obtain 6,000,000,000/gram of 500 grams, 2% kasugarnycin bacillus licheniformis wetting powder.
Embodiment 11
With the former powder of bacillus licheniformis (30,000,000,000 living spores/grams) 167 grams, the former powder of Trichoderma (5,000,000,000 spore alive/grams) 30 grams, filler (kaolin and sodium sulphate mix at 1: 1) 293 grams, other 10 gram (surfactant lignin sulfonic acid sodium salt 5 grams, dispersant alkyl sulfonic acid sylvite 5 grams), mixed grinding can get 10,000,000,000 of 500 grams/300,000,000 spore alive/gram Trichoderma soluble powders of gram bacillus licheniformis.
Embodiment 12
With the former powder of bacillus licheniformis (30,000,000,000 living spores/grams) 100 grams, former powder 17 grams of 60% Ningnanmycin, filler (kaolin and sodium sulphate mix at 1: 1) 363 grams, other 20 gram (surfactant lignin sulfonic acid sodium salt 10 grams, dispersant alkyl sulfonic acid sylvite 10 grams), mixed grinding can get 6,000,000,000/gram of 500 grams, 2% Ningnanmycin bacillus licheniformis soluble powder.
Embodiment 13
With the former powder of bacillus licheniformis (30,000,000,000 living spores/grams) 100 grams, former powder 316 grams of 95% jinggangmeisu, filler (kaolin and sodium sulphate mix at 1: 1) 64 grams, other 20 gram (surfactant lignin sulfonic acid sodium salt 10 grams, dispersant alkyl sulfonic acid sylvite 10 grams), mixed grinding can get 6,000,000,000/gram of 500 grams, 60% jinggangmeisu bacillus licheniformis soluble powder.
Embodiment 14
With the former powder of bacillus licheniformis (30,000,000,000/gram) 100 grams, former powder 263 grams of 95% tmtd draw back powder 25 grams, dispersing agent NNO 50 grams, precipitated calcium carbonate 62 grams after grinding after mixing, obtain 6,000,000,000/gram of 500 grams, 50% tmtd bacillus licheniformis wetting powder.
Embodiment 15
With the former powder of bacillus licheniformis (30,000,000,000/gram) 100 grams, former powder 96%208 grams of dimethomorph draw back powder 25 grams, dispersing agent NNO _ 50 grams, precipitated calcium carbonate 117 grams after grinding after mixing, obtain 6,000,000,000 of 40% dimethomorphs/gram bacillus licheniformis wetting powder 500 grams.
Embodiment 16
Claim 96% hymexazo former powder 312 grams, 30,000,000,000/ml the lichen bacillus ferments stoste, 100 grams, emulsifier 0203B25 gram and water 63 grams.Earlier the former powder of hymexazo is added in the lichen bacillus ferments stoste, the limit edged stirs, and adds 0203B after the dissolving while stirring, after adding water after again and supplying 500 grams and stir, promptly makes 6,000,000,000 living spores of 60% hymexazo/ml bacillus licheniformis aqua 500 grams.
Embodiment 17
Claim 90% Propamocarb stoste 388 grams, 60,000,000,000/ml the lichen bacillus ferments concentrates stoste 50 grams, emulsifier 0203B25 gram and water 37 grams.Earlier Propamocarb stoste is mixed with the lichen bacillus ferments stoste, stirs, add 0203B25 then and restrain while mixing, add after again water supply 500 restrain stir after, promptly make 6,000,000,000 living spores of 70% Propamocarb/ml bacillus licheniformis aqua 500 and restrain.
Embodiment 18
Claim to give birth in 90% rhzomorph stoste 222 grams, 60,000,000,000/ml the lichen bacillus ferments concentrates stoste 50 grams, emulsifier 0203B25 gram and water 203 grams.Earlier middle living rhzomorph stoste is mixed with the lichen bacillus ferments stoste, stirs, fully mix the back and add 0203B while mixing, add after again water supply 500 restrain stir after, promptly make 6,000,000,000 living spores of living rhzomorph in 40%/ml bacillus licheniformis aqua 500 and restrain.
Embodiment 19
Accurately weigh up former powder 10.5 grams of 95% jinggangmeisu, add in 16,000,000,000 living spores/ml the lichen bacillus ferments liquid 250 grams, claim 30,000,000,000 living spores/ml bacillus subtilis stoste 83 grams to add in the mixed liquor again, stir, add emulsifier 0203B 25 grams subsequently, remaining water is supplied 500 grams, fully makes 5,000,000,000 living spores of 8,000,000,000/ml of 2% jinggangmeisu bacillus licheniformis/ml bacillus subtilis aqua 500 grams behind the mixed dissolution.
Embodiment 20
With the former powder of bacillus licheniformis (30,000,000,000/gram) 100 grams, former powder 263 grams of 95% fenaminosulf draw back powder 25 grams, dispersing agent NNO 50 grams, precipitated calcium carbonate 62 grams after grinding after mixing, obtain 6,000,000,000/gram of 500 grams, 50% fenaminosulf bacillus licheniformis wetting powder.
Embodiment 21
Claim 90% amino-oligosaccharide stoste 11 grams, 60,000,000,000/ml the lichen bacillus ferments concentrates stoste 50 grams, emulsifier 0203B 25 grams and water 414 grams.Earlier amino-oligosaccharide stoste is mixed with the lichen bacillus ferments stoste, stir while mixing, add 0203B_25 gram then, after adding water after again and supplying 500 grams and stir, promptly make 6,000,000,000 living spores of 2% amino-oligosaccharide/ml bacillus licheniformis aqua 500 grams.
Embodiment 22
With the former powder of bacillus licheniformis (30,000,000,000 living spores/grams) 100 grams, former powder 158 grams of 95% doxycycline monohydrate, filler (kaolin and sodium sulphate mix at 1: 1) 222 grams, other 20 gram (surfactant lignin sulfonic acid sodium salt 10 grams, dispersant alkyl sulfonic acid sylvite 10 grams), mixed grinding can get 6,000,000,000/gram of 500 grams, 30% doxycycline monohydrate bacillus licheniformis soluble powder.
Embodiment 23
Claim 90% antibiotic in pyrimidine nucleoside class stoste 56 grams, 60,000,000,000/ml the lichen bacillus ferments concentrates stoste 50 grams, emulsifier 0203B 25 grams and water 369 grams.Earlier antibiotic in pyrimidine nucleoside class stoste is mixed with the lichen bacillus ferments stoste, stir while mixing, fully mix the back and add 0203B, after adding water after again and supplying 500 grams and stir, promptly make 6,000,000,000 living spores of 10% antibiotic in pyrimidine nucleoside class/ml bacillus licheniformis aqua 500 grams.
Embodiment 24
With the former powder of bacillus licheniformis (30,000,000,000 living spores/grams) 167 grams, the former powder of Paenibacillus polymyxa (5,000,000,000 spore alive/grams) 5 grams, filler (kaolin and sodium sulphate mix at 1: 1) 318 grams, other 10 gram (surfactant lignin sulfonic acid sodium salt 5 grams, dispersant alkyl sulfonic acid sylvite 5 grams), mixed grinding can get 10,000,000,000 of 500 grams/0.5 hundred million spore alive/gram Paenibacillus polymyxa soluble powders of gram bacillus licheniformis.
Embodiment 25
With the former powder of bacillus licheniformis (30,000,000,000 living spores/grams) 167 grams, the former powder of Pseudomonas fluorescence (10,000,000,000 spore alive/grams) 50 grams, filler (kaolin and sodium sulphate mix at 1: 1) 363 grams, other 10 gram (surfactant lignin sulfonic acid sodium salt 5 grams, dispersant alkyl sulfonic acid sylvite 5 grams), mixed grinding can get 10,000,000,000 of 500 grams/1,000,000,000 spore alive/gram Pseudomonas fluorescence soluble powders of gram bacillus licheniformis.
From 2002, we carried out a large amount of demonstration tests with the resulting compound medicine of the foregoing description in all parts of the country, the results are shown in Table 1 to table 8.
Table 1, prevent and treat the efficiency test result such as the following table of rice sheath blight disease with the goods of embodiment 1 and embodiment 13, embodiment 19:
| Handle | Dosage | Rice sheath blight disease disease index % | Preventive effect % |
| Embodiment 1 aqua | 200 times of liquid | 8.5 | 81.36 |
| Embodiment 13 soluble powders | 600 times of liquid | 6.5 | 85.75 |
| Embodiment 19 aquas | 300 times of liquid | 5.2 | 88.60 |
| CK | - | 45.6 | - |
Table 2, to prevent and treat the efficiency test result of angular leaf spot of cucumber with the goods of embodiment 3, embodiment 10, embodiment 18 and embodiment 22 as follows:
| Handle | Dosage | Angular leaf spot of cucumber disease index % | Preventive effect % |
| Embodiment 3 aquas | 400 times of liquid | 9.8 | 81.58 |
| Embodiment 18 aquas | 800 times of liquid | 9.2 | 82.71 |
| Embodiment 10 wetting powders | 600 times of liquid | 10.6 | 80.08 |
| Embodiment 22 soluble powders | 800 times of liquid | 8.6 | 83.83 |
| CK | - | 53.2 | - |
The goods control cucumber downy mildew result of the test of table 3, usefulness embodiment 2, embodiment 4, embodiment 5, embodiment 6, embodiment 7, embodiment 15, embodiment 16 and embodiment 17:
| Handle | Dosage | Disease index % | Preventive effect % |
| Embodiment 2 aquas | 400 times | 16.6 | 78.22 |
| Embodiment 4 aquas | 500 times | 18.3 | 75.98 |
| Embodiment 5 aquas | 300 times | 12.6 | 83.46 |
| Embodiment 6 wetting powders | 200 times | 19.6 | 74.28 |
| Embodiment 7 wetting powders | 600 times | 12.0 | 84.25 |
| Embodiment 15 wetting powders | 1000 times | 9.8 | 87.14 |
| Embodiment 16 wetting powders | 1000 times | 13.2 | 82.68 |
| Embodiment 17 wetting powders | 800 times | 10.2 | 86.61 |
| CK | - | 76.2 | - |
The efficiency test result of the goods control watermelon leaf pinta of table 4, usefulness embodiment 8, embodiment 9 and embodiment 14:
| Handle | Dosage | Disease index % | Preventive effect % |
| Embodiment 8 wetting powders | 400 times | 9.8 | 85.2 |
| Embodiment 9 wetting powders | 400 times | 12 | 81.8 |
| Embodiment 14 wetting powders | 600 times | 8.6 | 87 |
| CK | - | 66.2 | - |
Table 5, prevent and treat the powdery mildew of cucumber result of the test with the goods of embodiment 12
| Handle | Dosage | Disease index % | Preventive effect % |
| Embodiment 12 soluble powders | 400 times | 15.2 | 79.1 |
| The 62.25% celestial wetting powder of giving birth to | 600 times | 15.6 | 78.5 |
| CK | - | 72.5 | - |
The goods control graw mold of tomato result of the test of table 6, usefulness embodiment 11
| Handle | Dosage | Disease index % | Preventive effect % |
| Embodiment 11 soluble powders | 300 times | 16.2 | 78.8 |
| CK | - | 76.3 | - |
The goods control watermelon blight result of the test of table 7, usefulness embodiment 20, embodiment 21 and embodiment 23
| Handle | Dosage | Disease index % | Preventive effect % |
| Embodiment 20 wetting powders | 300 times of liquid | 8.6 | 85.27 |
| Embodiment 21 aquas | 400 times of liquid | 12.9 | 77.91 |
| Embodiment 23 aquas | 500 times of liquid | 9.8 | 83.22 |
| CK | - | 58.4 | - |
The goods control of plant bacterial wilt result of the test of table 8, usefulness embodiment 24 and embodiment 25
| Handle | Dosage | Disease index % | Preventive effect % |
| Embodiment 24 soluble powders | 300 times of liquid | 8.6 | 85.71 |
| Embodiment 25 soluble powders | 400 times of liquid | 12.9 | 78.57 |
| CK | - | 60.2 | - |
Claims (2)
1. a class contains the composite bactericide of bacillus licheniformis, it is characterized in that bacillus licheniformis and a kind of of biological bactericide or chemical bactericide or two kinds are mixed into regular dosage form by a certain percentage to be used; Described biological bactericide or chemical bactericide are meant a kind of in jinggangmeisu, middle living rhzomorph, polyoxin, Ningnanmycin, kasugarnycin, Trichoderma, Propamocarb, dimethomorph, carbendazim, tmtd, hymexazo, fenaminosulf, cytosintetidemycin, doxycycline monohydrate, amino-oligosaccharide, Fluoxastrobin, antibiotic in pyrimidine nucleoside class, Paenibacillus polymyxa, Pseudomonas fluorescence, bacillus subtilis, the Bacilluscereus, or two kinds.
2, the composite bactericide that contains bacillus licheniformis as claimed in claim 1 is characterized in that living spores number 〉=8 * 10 that described bacillus licheniformis contains
7Individual/gram or ml; It is 0.5～95.0% that composite composition contains jinggangmeisu by the weight of formulation ratio, in mildew plain 0.5～99.9%, polyoxin is 0.1～95.0%, Ningnanmycin is 0.5～95.0%, kasugarnycin 0.5～95.0%, Propamocarb 0.5～95.0%, dimethomorph 0.5～95.0%, carbendazim 0.5～95.0%, tmtd 0.5～99.9% ， hymexazo 0.5～95.0%, fenaminosulf 0.5～95.0%, cytosintetidemycin 0.5～95.0%, doxycycline monohydrate 0.5～95.0%, amino-oligosaccharide 0.5～95.0%, Fluoxastrobin 0.5～95.0%, antibiotic in pyrimidine nucleoside class 0.5～95.0%, bacillus subtilis living spores number 〉=5 * 10
7Individual/gram or ml, Paenibacillus polymyxa 5 * 10
6Individual/gram or ml, Pseudomonas fluorescence 5 * 10
6Individual/gram or ml, waxy Bacillus living spores number 〉=5 * 10
7Individual/gram or ml, Trichoderma 〉=5 * 10
7Individual/gram or ml.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610124711 CN1930991A (en) | 2006-10-01 | 2006-10-01 | Re-compounded germicide containing Bucillus licheniformis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610124711 CN1930991A (en) | 2006-10-01 | 2006-10-01 | Re-compounded germicide containing Bucillus licheniformis |
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| Publication Number | Publication Date |
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| CN1930991A true CN1930991A (en) | 2007-03-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610124711 Pending CN1930991A (en) | 2006-10-01 | 2006-10-01 | Re-compounded germicide containing Bucillus licheniformis |
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