CN117694352A - Hymexazol composition special for preventing and treating diseases and insects in soil and application thereof - Google Patents
Hymexazol composition special for preventing and treating diseases and insects in soil and application thereof Download PDFInfo
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- CN117694352A CN117694352A CN202410163852.6A CN202410163852A CN117694352A CN 117694352 A CN117694352 A CN 117694352A CN 202410163852 A CN202410163852 A CN 202410163852A CN 117694352 A CN117694352 A CN 117694352A
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- 239000005794 Hymexazol Substances 0.000 title claims abstract description 105
- KGVPNLBXJKTABS-UHFFFAOYSA-N hymexazol Chemical compound CC1=CC(O)=NO1 KGVPNLBXJKTABS-UHFFFAOYSA-N 0.000 title claims abstract description 105
- 239000000203 mixture Substances 0.000 title claims abstract description 64
- 201000010099 disease Diseases 0.000 title claims abstract description 42
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 42
- 239000002689 soil Substances 0.000 title claims abstract description 37
- 241000238631 Hexapoda Species 0.000 title claims abstract description 33
- 241000199919 Phaeophyceae Species 0.000 claims abstract description 112
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 112
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 112
- 238000002360 preparation method Methods 0.000 claims abstract description 37
- 244000068988 Glycine max Species 0.000 claims abstract description 27
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 27
- 241000219112 Cucumis Species 0.000 claims abstract description 26
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims abstract description 26
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims abstract description 20
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims abstract description 19
- 241000223218 Fusarium Species 0.000 claims abstract description 19
- 241000243785 Meloidogyne javanica Species 0.000 claims abstract description 15
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 13
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims abstract description 12
- 238000009210 therapy by ultrasound Methods 0.000 claims description 26
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 20
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 14
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 14
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 14
- 239000000945 filler Substances 0.000 claims description 12
- 235000018645 Allium odorum Nutrition 0.000 claims description 10
- 235000005338 Allium tuberosum Nutrition 0.000 claims description 10
- 244000000383 Allium odorum Species 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- 238000012986 modification Methods 0.000 claims description 7
- 230000004048 modification Effects 0.000 claims description 7
- 230000003285 pharmacodynamic effect Effects 0.000 claims description 7
- 230000000857 drug effect Effects 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- 238000009472 formulation Methods 0.000 claims description 3
- 235000001270 Allium sibiricum Nutrition 0.000 abstract description 13
- 230000002045 lasting effect Effects 0.000 abstract description 8
- 230000002195 synergetic effect Effects 0.000 abstract description 6
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- 230000010496 root system development Effects 0.000 abstract 1
- 238000011282 treatment Methods 0.000 description 183
- 230000000694 effects Effects 0.000 description 84
- 241000196324 Embryophyta Species 0.000 description 26
- 230000000052 comparative effect Effects 0.000 description 26
- 230000002265 prevention Effects 0.000 description 25
- 238000012360 testing method Methods 0.000 description 22
- 241000244206 Nematoda Species 0.000 description 17
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- 238000004364 calculation method Methods 0.000 description 14
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 244000305550 Streptopus amplexifolius Species 0.000 description 6
- 235000001231 Streptopus amplexifolius Nutrition 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
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- 230000001988 toxicity Effects 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
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- 238000007619 statistical method Methods 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241000243786 Meloidogyne incognita Species 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
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- RJGDLRCDCYRQOQ-UHFFFAOYSA-N anthrone Chemical compound C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 RJGDLRCDCYRQOQ-UHFFFAOYSA-N 0.000 description 1
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- 239000005645 nematicide Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The application discloses a special hymexazol composition for preventing and treating diseases and insects in soil and application thereof. The modified brown algae polyphenols and the hymexazol are mutually synergistic, the dosage of the hymexazol can be obviously reduced, the quick acting performance and the lasting period of the hymexazol on soil-borne diseases and insects can be obviously improved, especially the cucumber root-knot nematode, soybean root rot, chives and chives maggots and melon fusarium wilt are more obvious, after the composition is prepared into a composition preparation and applied to crops, the soil environment can be competed, the soil diseases and insect problems are solved, the root system development, the crop robustness, the quality improvement and the yield increase of the crops are realized, and the soil environment is friendly, safe, green and environment-friendly.
Description
Technical Field
The application relates to the technical field of pesticides, in particular to a special hymexazol composition for preventing and treating diseases and insects for soil and application thereof.
Background
At present, chemical pesticides are mainly used for preventing and controlling soil-borne diseases and insects, pathogenic bacteria can be effectively inhibited in a short period, but with the enhancement of the drug resistance of pathogenic bacteria, the dosage of the pesticides is continuously increased, so that more serious disease outbreaks are caused, and soil pollution and ecological environment harm are caused.
The hymexazol is a systemic bactericide, can be absorbed by roots of plants and moves in the roots of the plants, is metabolized in the plants to generate two kinds of glycoside, has the effect of improving physiological activity on crops and promoting root elongation, is an excellent soil disinfectant, is efficient on pathogenic bacteria in soil, and can inhibit germ spore germination after being applied to the soil. However, the hymexazol has high water solubility, is easy to run off and leach in soil, so that the acting duration is short, and pathogenic bacteria are easy to generate drug resistance to the hymexazol after long-term use, thereby seriously affecting the application of the hymexazol in preventing and treating soil diseases and insects. Therefore, how to improve the drug effect and reduce the use amount of hymexazol is a problem to be solved.
Disclosure of Invention
In view of the above, the application provides a soil-specific disease and insect prevention hymexazol composition and application thereof, wherein the composition comprises hymexazol and modified brown algae polyphenols. The modified brown algae polyphenols and the hymexazol are mutually synergistic, the dosage of the hymexazol can be obviously reduced, the quick acting property and the lasting period of the hymexazol on soil-borne diseases and insect pests can be improved, and the modified brown algae polyphenols and the hymexazol are more obvious in particular to cucumber root knot nematodes, soybean root rot, leek and leek maggots and melon fusarium wilt. After the composition is prepared into a composition preparation and applied to crops, the soil environment can be competed, the problems of soil diseases and insects are solved, the purposes of developed root systems of crops, strong crops, quality improvement and yield increase are achieved, and the composition is environment-friendly, safe, green and environment-friendly to the soil.
In a first aspect, the application provides a soil-specific anti-disease and insect hymexazol composition, the composition comprising a pharmacodynamic component comprising hymexazol and modified brown algae polyphenols. According to the application, the hymexazol and the modified brown algae polyphenol are combined, through the conduction effect of the plant body, the composition can reach all parts of the plant body, has obvious synergistic effect, has the effect of improving the physiological activity of crops, and can activate the plant body to generate antibacterial and insect-resistant substances, so that the quick acting performance and the lasting period are further improved.
In some embodiments, the mass ratio of the hymexazol to the modified brown algae polyphenols is (0.1-1): 1-10. The mass ratio of the hymexazol to the modified brown algae polyphenol is regulated and controlled within the range, so that the plant body is activated to generate antibacterial and insect-resistant substances, the soil antibacterial effect and the plant body insect-resistant effect are improved in both directions, and the duration is prolonged. Preferably, the mass ratio of the hymexazol to the modified brown algae polyphenol is (0.25-0.75): 2.5-5.
In some embodiments, the modified brown algae polyphenols are modified by using ammonium sulfate as a carrier. The modification comprises heating brown algae polyphenols to 40-50deg.C, performing first ultrasonic treatment, mixing the ultrasonic treated brown algae polyphenols with ammonium sulfate according to mass ratio of 10:1, and performing second ultrasonic treatment. Wherein the ultrasonic treatment time is 1 h-1.5 h, and the frequency is 20 kHz-40 kHz. The modified brown algae polyphenol obtained by modification through the method is more favorable for being matched with the hymexazol in a synergistic way, and the use amount of the hymexazol can be obviously reduced while the drug effect of the composition is improved.
In a second aspect, the application provides application of the hymexazol composition in preventing and controlling soil diseases and insects, wherein the application comprises the step of preparing the hymexazol composition into a hymexazol composition preparation. The hymexazol composition preparation comprises a medicinal component and a filler, wherein the medicinal component comprises hymexazol and modified brown algae polyphenol, and the filler comprises kelp residue, wherein the kelp residue is a main filler and has no influence on medicinal effects. Based on the composition preparation, the mass percentage of each component is as follows: the drug effect component is 1-15 wt% and the balance is filler. The application further prepares the composition containing the pharmacodynamic components into a composition preparation and applies the composition preparation into the soil, and simultaneously controls the proportion of the pharmacodynamic components and the filler to be proper, at the moment, the pesticide effect control method can achieve the purposes of improving the pest resistance of crops and killing pathogenic bacteria of the soil, avoiding soil pollution and reducing the optimal state of the soil.
In some embodiments, the mass ratio of the hymexazol to the modified brown algae polyphenols is (0.1-1): 1-10. At this time, the quick-acting property and the lasting period of the composition preparation are facilitated to be improved. Preferably, the mass ratio of the hymexazol to the modified brown algae polyphenol is (0.25-0.75): 2.5-5. When the modified brown algae polyphenol and a proper amount of hymexazol are combined and prepared into a preparation to be applied to soil, the composition preparation can be combined with soil metal ions to inhibit the germination of spores and the normal growth of pathogenic mycelium or directly kill germs, inhibit the activity of cells, prevent the cells from dividing and growing, interfere reproductive behaviors, influence the formation of eggs and solve the problems of soil-borne diseases and insects from the source.
In some embodiments, the formulation of the hymexazol composition described above is applied as a 10 kg/mu ditch.
In some embodiments, the insect pest comprises cucumber root knot nematodes and/or chives maggots, and the soil disease comprises soybean root rot and/or melon fusarium wilt.
The technical scheme provided by some embodiments of the present application has the beneficial effects that at least includes:
1) In the application, by combining the hymexazol and the modified brown algae polyphenols, the quick acting property and the lasting period of the hymexazol on soil diseases and insects can be obviously improved while the using amount of the hymexazol is reduced, and especially the cucumber root knot nematode, the soybean root rot, the chives and chives maggots and the melon fusarium wilt are more obvious.
2) The application further prepares the composition containing the hymexazol and the modified brown algae polyphenol into a composition preparation, and applies the composition preparation to soil, so that on one hand, the effect of killing soil pathogenic bacteria while improving the insect resistance of crops can be achieved, and the soil pollution can not be caused, on the other hand, the composition preparation can be combined with soil metal ions to inhibit the germination of spores and the normal growth of pathogenic mycelium or directly kill germs, inhibit the activity of cells, prevent the cells from dividing and growing, interfere reproductive behaviors, influence the formation of eggs, and solve the problems of soil diseases and insects from the source.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application will be further described in detail with reference to examples. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the present application.
Hymexazol composition
The pesticide comprises a pesticide effect component, wherein the pesticide effect component comprises hymexazol and modified brown algae polyphenol, and the mass ratio of the hymexazol to the modified brown algae polyphenol is (0.1-1) (1-10). Illustratively, the mass ratio of hymexazol to modified brown algae polyphenols is 0.25:7.5, 0.5:5, 0.75:2.5, or a range of any two of the foregoing values.
In some embodiments, the modified brown algae polyphenols are obtained by modifying brown algae polyphenols with ammonium sulfate as a carrier, the modification comprises heating the brown algae polyphenols to 40-50 ℃ and performing a first ultrasonic treatment, mixing the brown algae polyphenols after the ultrasonic treatment with ammonium sulfate according to a mass ratio of 10:1, and performing a second ultrasonic treatment. Wherein the ultrasonic treatment time is 1 h-1.5 h, and the frequency is 20 kHz-40 kHz. Illustratively, brown algae polyphenols are heated to 40 ℃, 43 ℃, 45 ℃, 47 ℃, 49 ℃, 50 ℃, or a range of any two values above.
In the embodiment of the application, the structural formula of the brown algae polyphenol is shown as formula I, and the brown algae polyphenol is purchased from Shandong Hengtai ocean Biotechnology limited company brown algae polyphenol stock solution.
I is a kind of
In the embodiment of the application, the structural formula of the hymexazol is shown as a formula II, and the hymexazol is 99% of original medicine purchased from Wisea Han Fu Biochemical pharmaceutical industry Co.
II type
Hymexazol composition preparation
Comprises a medicinal component and a filler, wherein the medicinal component comprises hymexazol and modified brown algae polyphenol, the filler comprises kelp residue, and preferably the filler is kelp residue.
In some embodiments, the composition comprises the following components in percentage by mass based on the preparation of the composition: the drug effect component is 1-15 wt% and the balance is filler. Illustratively, the content of the pharmacodynamic component is 1wt%, 2wt%, 3wt%, 5wt%, 5.5wt%, 8wt%, 10wt%, 10.1wt%, 12wt%, 13wt%, 15wt%, or a range of any two of the above values.
In some embodiments, the mass ratio of the hymexazol to the modified brown algae polyphenols is (0.1-1): 1-10. Illustratively, the mass ratio of hymexazol to modified brown algae polyphenols is 0.25:7.5, 0.5:5, 0.75:2.5, or a range of any two of the foregoing values.
In some embodiments, the modified brown algae polyphenol is formed by modifying brown algae polyphenol by taking ammonium sulfate as a carrier, wherein the modification comprises heating the brown algae polyphenol to 40-50 ℃, performing first ultrasonic treatment, mixing the ultrasonic treated brown algae polyphenol with ammonium sulfate according to a mass ratio of 10:1, and performing second ultrasonic treatment, wherein the ultrasonic treatment time is 1-1.5 h, and the frequency is 20-40 kHz.
Modification method of brown algae polyphenol
S100, heating brown algae polyphenols to 40-50 ℃ and performing first ultrasonic treatment;
s200, mixing the brown algae polyphenol processed in the step S100 with ammonium sulfate according to the mass ratio of 10:1, and performing second ultrasonic treatment to obtain an intermediate sample;
wherein the ultrasonic treatment time is 1 h-1.5 h, and the frequency is 20 kHz-40 kHz;
s300, centrifuging and concentrating the intermediate sample in the step S200 according to the mass ratio of the feed liquid of 1:9.
In step S200, the brown algae polyphenols processed in step S100 may be mixed with ammonium sulfate during the second ultrasonic treatment, which is not limited in this application.
Examples
The following examples more particularly describe the disclosure of the present application, which are intended as illustrative only, since numerous modifications and variations within the scope of the disclosure will be apparent to those skilled in the art. Unless otherwise indicated, all parts, percentages, and ratios reported in the examples below are by weight, and all reagents used in the examples are commercially available or were obtained synthetically according to conventional methods and can be used directly without further treatment, as well as the instruments used in the examples.
Preparation of modified brown algae polyphenol
(1) Modified brown algae polyphenol A
Heating the brown algae polyphenol stock solution to 40 ℃ and performing first ultrasonic treatment, continuously adding ammonium sulfate into the brown algae polyphenol subjected to the first ultrasonic treatment under the condition of second ultrasonic treatment, wherein the mass ratio of the brown algae polyphenol to the ammonium sulfate is 10:1, the time of the two ultrasonic treatments is 1.5h, the frequency is 20KHZ, and finally, performing centrifugal concentration by using a disc centrifuge according to the mass ratio of feed liquid of 1:9, thereby obtaining the modified brown algae polyphenol A.
(2) Modified brown algae polyphenols B
Heating the brown algae polyphenol stock solution to 50 ℃ and performing first ultrasonic treatment, continuously adding ammonium sulfate into the brown algae polyphenol subjected to the first ultrasonic treatment under the condition of second ultrasonic treatment, wherein the mass ratio of the brown algae polyphenol to the ammonium sulfate is 10:1, the time of the two ultrasonic treatments is 1h, the frequency is 40KHZ, and finally, performing centrifugal concentration by using a disc centrifuge according to the mass ratio of feed liquid of 1:9, thereby obtaining the modified brown algae polyphenol B.
Hymexazol composition
Example 1
This example is described in section 1 of the standard nematicide for in-house bioassay tests for pesticides, with reference to NY/T1833.1-2009: the test insect dipping method for inhibiting plant pathogenic nematodes is used for the combined toxicity measurement of hymexazol and modified brown algae polyphenol on meloidogyne incognita.
Test subject: separating from root-knot samples of cucumber in the greenhouse of Shenxian county to obtain the root-knot nematode of south, and culturing and breeding the root-knot nematode on living cucumber plants in a laboratory.
And (3) preparation of a medicament: adopts 99% of hymexazol original drug and modified brown algae polyphenol A. Each of the crude drugs was dissolved in acetone and then diluted with 0.1% tween-80 aqueous solution. Each agent was set up with 5 series of concentration gradients, with the same solvent content in aqueous solution without agent as a control.
Preparation of nematode suspension: picking root-knot nematode egg masses from cucumber root knots with serious root-knot nematode disease, cleaning with clear water, placing on filter paper in a culture dish, adding a proper amount of distilled water, placing in a culture box with 25+/-1 ℃ for incubation and culture, collecting second-instar larvae with consistent instar after 24 hours, and preparing into root-knot nematode suspension, wherein each 1mL of suspension contains at least 100 nematodes for standby.
The test method comprises the following steps: sequentially sucking 3mL of liquid medicine from low concentration to high concentration by a pipette, respectively adding the liquid medicine into a test tube, and then sucking 3mL of prepared equivalent nematode suspension into the test tube, so that the liquid medicine and the nematode suspension are uniformly mixed in equivalent. And (3) transferring a certain volume of the mixed solution into the small holes of the porous biochemical test plate by using a pipetting gun, capping, repeating the treatment for 4 times, and taking an aqueous solution which does not contain a medicament and has the same solvent content as a control. Culturing at 25 ℃ for 24 hours, checking the activity condition of nematodes, and calculating the relative mortality rate of each medicament treatment to the nematodes.
Experimental investigation: 1mL of the mixed solution is taken from each treatment, the death condition of the nematodes is observed under a dissecting mirror, and the number of the nematodes observed repeatedly is not less than 100. The total number of nematodes investigated and the number of dead nematodes were recorded. Dead nematodes are abnormally stiff and cannot bend when touched by a hairpin or a bamboo filament pin.
The calculation method comprises the following steps: according to the investigation data, the mortality of each treatment is calculated, the calculation is carried out according to the following formula, and the calculation results are reserved to two positions after decimal points:
if the control mortality is less than 5%, correction is not needed; if the contrast mortality is between 5% and 10%, correcting according to a correction mortality formula; control mortality was greater than 15% and the test required reworking.
Statistical analysis: according to the logarithmic value of each medicament concentration and the corresponding mortality, the effect of the pesticide is evaluated by using software such as a Statistical Analysis System (SAS) and a Data Processing System (DPS), and the effect of the pesticide is evaluated by using a grand cloud Peel method, the toxicity LC50 of the pesticide is measured, and the relative toxicity index and the co-toxicity coefficient are calculated according to the following calculation formula. Wherein, the co-toxicity coefficient is more than 120, which is a synergistic effect; the co-toxicity coefficient is between 80 and 120, and is the superposition effect; the co-toxicity coefficient is smaller than 80, and the antagonism is realized. The compound with a toxicity index of 100 was designated as a standard insecticide, the invention was selected as hymexazol, and the results are shown in table 1.
TABLE 1
As can be seen from Table 1, the hymexazol and the modified brown algae polyphenols are combined and synergistically enhanced when the mass ratio is controlled within the range of (0.1-1): 1-10, and the co-toxicity coefficient is more than 120. When the mass ratio of the hymexazol to the modified brown algae polyphenol is within the range of (0.25-0.75): (2.5-5), the co-toxicity coefficient of the hymexazol and the modified brown algae polyphenol is above 150, and the synergistic effect is obvious. When the mass ratio of the hymexazol to the modified brown algae polyphenol is 0.5:5, the co-toxicity coefficient is the largest and is 171.88.
(Di) hymexazol composition
Example 2
A hymexazol composition preparation comprises 5% brown algae polyphenols, 0.5% hymexazol, and herba Zosterae Marinae residue to 100%.
Example 3
A hymexazol composition preparation comprises 1% of modified brown algae polyphenol A,1% of hymexazol and 100% of kelp residue.
Example 4
A hymexazol composition preparation comprises 5% of modified brown algae polyphenol A,0.5% of hymexazol and 100% of kelp residue.
Example 5
A hymexazol composition preparation comprises 10% of modified brown algae polyphenol A,0.1% of hymexazol and 100% of kelp residue.
Example 6
A hymexazol composition preparation comprises 1% of modified brown algae polyphenol B,1% of hymexazol and 100% of kelp residue.
Example 7
A hymexazol composition preparation comprises 5% of modified brown algae polyphenol B,0.5% of hymexazol and 100% of kelp residue.
Example 8
A hymexazol composition preparation comprises 10% of modified brown algae polyphenol B,0.1% of hymexazol and 100% of kelp residue.
Comparative example 1
A preparation comprises 10% modified brown algae polyphenols A, and herba Zosterae Marinae residue to 100%.
Comparative example 2
A preparation comprises 10% of modified brown algae polyphenols B and 100% of kelp residue.
Comparative example 3
A preparation comprises hymexazol 1% and kelp residue to 100%.
Comparative example 4
A preparation comprises brown algae polyphenols 10% and kelp residue 100%.
Comparative example 5
A preparation is prepared from 100% of kelp residue.
Application example 1
Taking a cucumber vegetable greenhouse with serious root-knot nematodes in chat county as a test base, performing the following experimental design, and verifying the control effect on the cucumber root-knot nematodes:
treatment 1: blank control (clear water);
treatment 2: example 2 samples were punched with furrows at 10 kg/mu;
treatment 3: example 3 samples were punched with furrows at 10 kg/mu;
treatment 4: example 4 samples were punched with furrows at 10 kg/mu;
treatment 5: example 5 samples were punched with furrows at 10 kg/mu;
treatment 6: example 6 samples were punched with furrows at 10 kg/mu;
treatment 7: example 7 samples were punched with furrows at 10 kg/mu;
treatment 8: example 8 samples were punched with furrows at 10 kg/mu;
treatment 9: the sample of comparative example 1 was applied with 10 kg/mu along with the ditch;
treatment 10: the sample of comparative example 2 was applied with 10 kg/mu with ditches;
treatment 11: the sample of comparative example 3 was applied with 10 kg/mu with ditches;
treatment 12: the sample of comparative example 4 was applied with 10 kg/mu with ditches;
treatment 13: the sample of comparative example 5 was applied with 10 kg/mu with ditches;
soil is turned over and spread for 1 time before transplanting, and 1 time is punched every 1 month after transplanting, and the total time is 3 times. Each treatment is provided with 3 repetition areas, which are not adjacent to each other. After the cucumber harvesting period is finished, a technician selects a five-point sampling method for each treatment area, 4 plants are selected for each point, the number of disease plants of each treatment stage is counted, then the disease index and the prevention and treatment effect of different treatments are calculated, and the grading standard of the cucumber root knot nematode disease is specifically as follows:
level 0 (no root knot);
level 1 (less than 5 root knots of the whole plant);
2 level (6-15 root knots of whole plant);
3 stages (16-25 root knots of the whole plant);
level 4 (26-50 root knots of whole plant);
grade 5 (more than 51 root knots of the whole plant).
And calculating the disease index and the prevention and treatment effect according to the severity, and performing the significance test of the difference of the prevention and treatment effect by using the disease index. The calculation formula is as follows, and the calculation result is shown in table 2:
table 2 questionnaires for disease index and control effect of cucumber root knot nematode by various treatments
Referring to table 2, from the test results, the control effect of the composition prepared samples (treatment 3-treatment 8) of the modified brown algae polyphenols and the hymexazol on the cucumber root-knot nematodes is obviously better than that of treatment 9-treatment 13, treatment 2 and treatment 1, which shows that the modified brown algae polyphenols can obviously improve the control effect of the hymexazol on the cucumber root-knot nematodes, and the highest control effect reaches 95.00%.
The control effect of the treatment 9 and the treatment 10 on the cucumber root-knot nematodes is obviously better than that of the treatment 12, which indicates that the control effect of the modified brown algae polyphenols on the cucumber root-knot nematodes is better than that of the modified brown algae polyphenols.
The control effect of treatments 3-8 on cucumber root-knot nematodes is obviously better than that of treatment 2, which indicates that the control effect of the modified brown algae polyphenols on the cucumber root-knot nematodes by the hymexazol is obviously better than that of the modified brown algae polyphenols.
The control effect of treatments 9-12 is better than that of treatments 1 (blank) and 13 (kelp residue), and the control effects of treatments 1 and 13 are basically unchanged.
Application example 2
The soybean planting field with serious root rot in Jiaxiang county of Shandong Jining is taken as a test base, and the following experimental design is carried out to verify the prevention and treatment effects on the soybean root rot:
treatment 1: blank control (clear water);
treatment 2: example 2 samples were punched with furrows at 10 kg/mu;
treatment 3: example 3 samples were punched with furrows at 10 kg/mu;
treatment 4: example 4 samples were punched with furrows at 10 kg/mu;
treatment 5: example 5 samples were punched with furrows at 10 kg/mu;
treatment 6: example 6 samples were punched with furrows at 10 kg/mu;
treatment 7: example 7 samples were punched with furrows at 10 kg/mu;
treatment 8: example 8 samples were punched with furrows at 10 kg/mu;
treatment 9: the sample of comparative example 1 was applied with 10 kg/mu along with the ditch;
treatment 10: the sample of comparative example 2 was applied with 10 kg/mu with ditches;
treatment 11: the sample of comparative example 3 was applied with 10 kg/mu with ditches;
treatment 12: the sample of comparative example 4 was applied with 10 kg/mu with ditches;
treatment 13: the sample of comparative example 5 was applied with 10 kg/mu with ditches;
soil turning and broadcasting are carried out for 1 time before transplanting, and 1 time is carried out every 1 month after transplanting, and the total time is 2 times. Each treatment is provided with 3 repetition areas, which are not adjacent to each other. After the soybean harvesting period is finished, a technician selects a five-point sampling method for each treatment area, 10 plants are selected for each point, the number of the disease plants of each treatment stage is counted, and then the disease index and the prevention and treatment effect of different treatments are calculated. Yield determination after harvest was performed, 4 spots were randomly taken for each treatment, 1m for each spot 2 The yield of soybeans was measured.
The soybean root rot grading standard refers to the 88 th part of the pesticide field efficacy test criterion (II) of GB/T17980.88-2004 pesticide field: the bactericide for preventing and treating soybean root rot is specifically as follows:
level 0: the plant stem base and the main root have no disease spots;
stage 1: a small amount of lesions are formed on the basal part and the main root of the stem;
3 stages: the base part or main root of the stem has more lesions, and the area of the lesions accounts for 1/4-1/2 of the total area of the stem and the root;
5 stages: the base and the main root of the stem have more and larger lesion spots, and the area of the lesion spots accounts for 1/2-3/4 of the total area of the base and the root of the stem;
7 stages: the disease spots on the basal part or the main root of the stem are connected to form a phenomenon of winding the stem, but the root system is not dead;
stage 9: and root system necrosis, wilting or death of the overground parts of plants.
And calculating the disease index and the prevention and treatment effect according to the severity, and performing the significance test of the difference of the prevention and treatment effect by using the disease index. The calculation formula is as follows, the calculation result is shown in table 3, and the yield measurement and yield result is shown in table 4:
TABLE 3 questionnaires for disease index and control effect of each treatment on soybean root rot
TABLE 4 Effect of various treatments on soybean yield questionnaires
From the test results, the results of the tables 3 and 4 show that the control effect of the modified brown algae polyphenol and hymexazol composition prepared by sample treatment 3-8 on soybean root rot is obviously better than that of the modified brown algae polyphenol prepared by sample treatment 9-13, treatment 2 and treatment 1, so that the control effect of the modified brown algae polyphenol on soybean root rot can be obviously improved, and the highest control effect can reach 96.37%.
Meanwhile, the sample (treatment 3-treatment 8) prepared from the modified brown algae polyphenol and hymexazol composition is obviously superior to treatment 9-13, treatment 2 and treatment 1 in terms of soybean yield increase, which shows that the modified brown algae polyphenol and hymexazol composition has obvious yield increase effect on soybean, and the maximum yield is increased by 27.20%.
The treatment 9 and the treatment 10 are obviously superior to the treatment 12 in the prevention and treatment effect of soybean root rot and soybean yield increase, which indicates that the modified brown algae polyphenol is superior to the unmodified brown algae polyphenol in the prevention and treatment effect of soybean root rot and soybean yield increase.
The treatment 3 to the treatment 8 are obviously superior to the treatment 2 in the aspects of preventing and controlling the soybean root rot and increasing the soybean yield, which shows that the modified brown algae polyphenol is obviously superior to the unmodified brown algae polyphenol in the aspects of improving the preventing and controlling the soybean root rot and increasing the soybean yield of the hymexazol.
In addition, the control effect of the soybean root rot and the soybean yield increase of the treatments 9-12 are superior to those of the treatments 1 (blank control) and 13 (kelp residues), and the control effects of the treatments 1 and 13 are basically indiscriminate.
Application example 3
The land for continuously planting the Chinese chives in the Changshan is taken as a test field, and the following experimental design is carried out to verify the prevention and control effects of the Chinese chives and Chinese chives.
Treatment 1: blank control (clear water);
treatment 2: example 2 samples were punched with furrows at 10 kg/mu;
treatment 3: example 3 samples were punched with furrows at 10 kg/mu;
treatment 4: example 4 samples were punched with furrows at 10 kg/mu;
treatment 5: example 5 samples were punched with furrows at 10 kg/mu;
treatment 6: example 6 samples were punched with furrows at 10 kg/mu;
treatment 7: example 7 samples were punched with furrows at 10 kg/mu;
treatment 8: example 8 samples were punched with furrows at 10 kg/mu;
treatment 9: the sample of comparative example 1 was applied with 10 kg/mu along with the ditch;
treatment 10: the sample of comparative example 2 was applied with 10 kg/mu with ditches;
treatment 11: the sample of comparative example 3 was applied with 10 kg/mu with ditches;
treatment 12: the sample of comparative example 4 was applied with 10 kg/mu with ditches;
treatment 13: the sample of comparative example 5 was applied with 10 kg/mu with ditches;
treatment 11: the sample of comparative example 4 was applied with 10 kg/mu with ditches;
soil turning and pesticide spreading are carried out once before planting, and then 1 time of spraying is carried out along with ditches every 1 month, and 2 times of spraying are carried out in total. Each treatment is provided with 3 repetition areas, which are not adjacent to each other. Refer to section 67 of the field efficacy test criteria (two): the method for preventing and controlling Chinese chives and root maggots by using the insecticide adopts a 5-point sampling method, wherein 3 mounds are investigated at each point, the number of insect population is firstly investigated before the insecticide is applied, the residual number of living insects after each treatment is respectively investigated on the 3 rd day, the 30 th day, the 60 th day and the harvest after the first insecticide is applied, the insect population is counted, the insect population reduction rate and the prevention and control effect are calculated, the calculation formula is shown as follows, and the result is shown in Table 5.
Table 5 questionnaires for reduction rate and control effect of the treated samples on the maggots of leeks and leeks
Referring to Table 5, by calculating and counting the reduction rate of insect population and the prevention and treatment effect before and after 3 days of application, the samples from treatment 3 to treatment 8 show better quick-acting effects on the Chinese chives and the prevention and treatment effects are all more than 86% and are better than the treatments from treatment 9 to treatment 13, treatment 2 and treatment 1. Wherein the control effect of the treatment 7 is best, and the control effect is 89.51%.
Through calculation and statistics of the reduction rate of insect population and the prevention and treatment effect before and after 30 days of pesticide application, the samples processed 3-processed 8 show good lasting effect on the Chinese chive maggots, and the prevention and treatment effects are more than 89% and are superior to those of the samples processed 9-processed 13, processed 2 and processed 1. Wherein the control effect of the treatment 7 is best, and the control effect is 91.95%.
Through calculation and statistics of the reduction rate of insect population and the prevention and control effect before and after 60 days of pesticide application, the samples processed 3-processed 8 show good lasting effect on the Chinese chive maggots, and the prevention and control effects are greater than 92% and are superior to those of the samples processed 9-processed 13, processed 2 and processed 1. Wherein the control effect of the treatment 7 is best, and the control effect is 94.55%.
Through calculation and statistics of the reduction rate of insect population and the prevention and control effect before and after pesticide application, the samples processed 3 to 8 show good lasting effect on the Chinese chive maggots, and the prevention and control effects are greater than 94% and are superior to those of the samples processed 9 to 13, 2 and 1. Wherein the control effect of the treatment 7 is the best, and the control effect is 96.35%.
All test treatment results show that the prevention and treatment effects of the treatment 9 and the treatment 10 on the chives and the chives are obviously better than those of the treatment 12, which shows that the prevention and treatment effects of the modified brown algae polyphenol on the chives and the chives are obviously better than those of the unmodified brown algae polyphenol.
The prevention and treatment effects of the treatments 3-8 on the chives and the chives are obviously better than those of the treatment 2, which shows that the prevention and treatment effects of the modified brown algae polyphenol on the chives and the chives are better than those of the unmodified brown algae polyphenol in improving the hymexazol.
In addition, the control effect of the treatments 9-12 is better than that of the treatments 1 (blank control) and 13 (kelp residues), and the control effects of the treatments 1 and 13 are basically unchanged.
Application example 4
The following experimental design is carried out by taking a melon greenhouse with serious wilt occurrence in the past year of shen county Yan shop town as an experimental base, and the control effect of the melon wilt is verified:
treatment 1: blank control (clear water);
treatment 2: example 2 samples were punched with furrows at 10 kg/mu;
treatment 3: example 3 samples were punched with furrows at 10 kg/mu;
treatment 4: example 4 samples were punched with furrows at 10 kg/mu;
treatment 5: example 5 samples were punched with furrows at 10 kg/mu;
treatment 6: example 6 samples were punched with furrows at 10 kg/mu;
treatment 7: example 7 samples were punched with furrows at 10 kg/mu;
treatment 8: example 8 samples were punched with furrows at 10 kg/mu;
treatment 9: the sample of comparative example 1 was applied with 10 kg/mu along with the ditch;
treatment 10: the sample of comparative example 2 was applied with 10 kg/mu with ditches;
treatment 11: the sample of comparative example 3 was applied with 10 kg/mu with ditches;
treatment 12: the sample of comparative example 4 was applied with 10 kg/mu with ditches;
treatment 13: the sample of comparative example 5 was applied with 10 kg/mu with ditches;
soil turning and broadcasting are carried out for 1 time before transplanting, and 1 time is carried out every 1 month after transplanting, and the total time is 2 times. Each treatment is provided with 3 repetition areas, which are not adjacent to each other.
Refer to section 113 of GB/T17980.113-2004 pesticide field efficacy test guidelines (II): the bactericide is used for preventing and treating melon fusarium wilt, and the typical symptoms of the fusarium wilt are investigated in all plants in each treatment area before application and in the fruit harvest period, and the number of fusarium wilt disease incidence plants and the total plant number are recorded. The test is investigated for 3 times, the disease rate and the prevention and treatment effect are calculated, the calculation formula is as follows, and the calculation result is shown in Table 6. The Vc content, the soluble sugar content and the yield of the fruits were investigated in the harvest period of the fruits, and the yield increase was calculated, wherein the Vc content was measured by a molybdenum blue colorimetric method, the soluble sugar content was measured by an anthrone colorimetric method, and the results are shown in Table 7.
Table 6 disease rate and control effect questionnaires of melon fusarium wilt for each treatment
TABLE 7 Effect of various treatments on melon Vc content, soluble sugar content and yield questionnaires
By combining the table 6 and the table 7, as can be seen from the test results before the first flushing, the plant disease rate and the control effect of the modified brown algae polyphenol and hymexazol composition prepared by sample treatment 3-8 on melon fusarium wilt are obviously better than those of treatment 9-13, treatment 2 and treatment 1, so that the modified brown algae polyphenol can obviously improve the control effect of the hymexazol on melon fusarium wilt, and the highest control effect reaches 94.74%.
As can be seen from the test results before the second flushing, the plant disease rate and the control effect of the sample preparation treatment 3-8 of the composition of the modified brown algae polyphenol and the hymexazol on melon fusarium wilt are obviously better than those of the sample preparation treatment 9-13, the modified brown algae polyphenol treatment 2 and the modified brown algae polyphenol treatment 1, so that the control effect of the hymexazol on the melon fusarium wilt can be obviously improved, and the final highest control effect reaches 97.37%.
The investigation test results in the harvest period show that the plant disease rate and the control effect of the sample preparation treatment 3-8 of the composition of the modified brown algae polyphenol and the hymexazol on melon fusarium wilt are obviously better than those of the treatment 9-13, the treatment 2 and the treatment 1, which shows that the modified brown algae polyphenol can obviously improve the control effect of the hymexazol on melon fusarium wilt, and the highest control effect finally reaches 98.08%.
All test treatment results show that the treatment effect of treatment 9 and treatment 10 on melon fusarium wilt is obviously better than that of treatment 12, which indicates that the treatment effect of modified brown algae polyphenols on melon fusarium wilt is obviously better than that of unmodified brown algae polyphenols. The control effect of the treatments 3-8 on melon fusarium wilt is obviously better than that of the treatment 2, which shows that the control effect of the modified brown algae polyphenol on the melon fusarium wilt is obviously better than that of the modified brown algae polyphenol in improving hymexazol.
In addition, the disease rate and the control effect of the treatments 8-12 are obviously better than those of the treatments 1 (blank control) and 13 (kelp residues), and the control effects of the treatments 1 and 13 are basically unchanged.
From the questionnaires of the Vc content, the soluble sugar content and the yield data of the fruits, the preparation of the composition of the modified brown algae polyphenol and the hymexazol for sample treatment 3-8 is obviously superior to treatment 9-13, treatment 2 and treatment 1 in the aspects of Vc content, the soluble sugar content, average mu yield and yield increase of melons, and the highest yield increase can reach 26.42%.
All test treatment results show that the treatment 9 and the treatment 10 are obviously superior to the treatment 12 in terms of the Vc content of fruits, the content of soluble sugar, the average acre yield and the yield increase rate of melons, and the modified brown algae polyphenol is obviously superior to the unmodified brown algae polyphenol in terms of the Vc content of fruits, the content of soluble sugar, the average acre yield and the yield increase rate of melons. The treatment 3 to the treatment 8 are obviously superior to the treatment 2 in terms of the Vc content of fruits, the soluble sugar content and the average acre yield and the yield increase of melons, which shows that the modified brown algae polyphenol is obviously superior to the unmodified brown algae polyphenol in terms of improving the Vc content of fruits, the soluble sugar content and the average acre yield and the yield increase of melons.
And meanwhile, the treatments 9-12 are superior to the treatments 1 (blank control) and 13 (kelp residues) in terms of Vc content, soluble sugar content and average mu yield and yield increase of melons, and basically no difference exists between the treatments 1 and 13.
The foregoing description of the preferred embodiment of the present invention is not intended to limit the invention to the particular form disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.
Claims (10)
1. The special hymexazol composition for preventing and treating diseases and insects in soil is characterized by comprising pharmacodynamic components, wherein the pharmacodynamic components comprise hymexazol and modified brown algae polyphenols.
2. The hymexazol composition as claimed in claim 1, wherein the mass ratio of the hymexazol to the modified brown algae polyphenols is (0.1-1): 1-10.
3. The hymexazol composition as claimed in claim 2, wherein the mass ratio of the hymexazol to the modified brown algae polyphenols is (0.25-0.75): 2.5-5.
4. The hymexazol composition according to any one of claims 1 to 3, wherein the modified brown algae polyphenols are modified by using ammonium sulfate as a carrier;
the modification comprises heating brown algae polyphenols to 40-50deg.C, and performing first ultrasonic treatment;
mixing the brown algae polyphenol subjected to ultrasonic treatment with ammonium sulfate according to the mass ratio of 10:1, and performing ultrasonic treatment for the second time;
wherein the ultrasonic treatment time is 1 h-1.5 h, and the frequency is 20 kHz-40 kHz.
5. The application of the hymexazol composition as claimed in any one of claims 1 to 4 in controlling soil diseases and insects, wherein the application comprises the preparation of the hymexazol composition into a hymexazol composition preparation.
6. The use according to claim 5, wherein the formulation of the hymexazol composition comprises a pharmacodynamic component and a filler;
the drug effect components comprise hymexazol and modified brown algae polyphenols;
the filler comprises kelp residue;
based on the hymexazol composition preparation, the mass percent of each component is as follows:
the drug effect component is 1-15 wt%;
and the balance of filler.
7. The use according to claim 6, wherein the mass ratio of the hymexazol to the modified brown algae polyphenols is (0.1-1): 1-10.
8. The use according to claim 7, wherein the mass ratio of the hymexazol to the modified brown algae polyphenols is (0.25-0.75): 2.5-5.
9. The use according to claim 5, wherein the formulation of the hymexazol composition is applied as a ditch of 10 kg/mu.
10. The use according to claim 5, wherein the soil diseases comprise soybean root rot and/or melon fusarium wilt;
the insect pest comprises cucumber root-knot nematode and/or Chinese chives and Chinese chives maggots.
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