CN116019101B

CN116019101B - Synergistic composition containing thymol for preventing and treating papaya mites

Info

Publication number: CN116019101B
Application number: CN202210967522.3A
Authority: CN
Inventors: 潘祖建; 陈燕; 陈仕淼; 余江敏; 郑剑; 何江; 欧景莉; 朱杨帆; 黄雪梅
Original assignee: Guangxi Subtropical Crops Research Institute
Current assignee: Guangxi Subtropical Crops Research Institute
Priority date: 2021-08-30
Filing date: 2021-08-30
Publication date: 2023-10-13
Anticipated expiration: 2041-08-30
Also published as: CN113632797B; CN113632797A; CN116019101A

Abstract

The invention relates to the technical field of pesticides, in particular to a synergistic composition containing thymol for controlling papaya pest mites. The synergistic composition comprises thymol, wherein the effective components of the synergistic composition are formed by binary combination of thymol and clofentezine; wherein the mass ratio of the clofentezine to the thymol is 1-5:10-1. When two effective components in the synergistic composition are compounded in a certain mass ratio, the co-toxicity coefficient is more than 120, and the synergistic effect is shown, so that the control effect on mites can be remarkably improved, the pesticide application amount is reduced, the control cost of the mites can be reduced, and the generation of drug resistance of the mites can be delayed; on the basis, the pesticide auxiliary materials acceptable in agriculture and pharmacy are added, so that the pesticide can be prepared into chemical agents, and the pesticide has important significance in developing novel chemical agents for preventing and removing harmful mites.

Description

Synergistic composition containing thymol for preventing and treating papaya mites

Technical Field

The invention relates to the technical field of pesticides, in particular to a synergistic composition containing thymol for controlling papaya pest mites.

Background

Papaya is a evergreen soft woody small arbor of the genus papaya of the family papaya. The mature papaya fruit can be used as fruit, the immature papaya fruit can be used as vegetable cooked food or pickled food, and can also be processed into products such as preserved fruit, fruit juice, jam and the like, in addition, the seeds can squeeze oil, and the fruits and leaves can be used for medicines.

As with crops, in the planting process of papaya, the papaya is often affected by diseases and insect pests, common diseases and insect pests include anthracnose, ring spot mosaic disease, downy mildew, pest mites, aphids, scale insects, prodenia litura and the like, wherein the pest mites are one of the main pests which harm the papaya, and mainly move on the back of papaya leaves to absorb juice, yellow spots are formed by the green-lacking and yellow-turning points of the pest leaves, and the yellow spots are connected into one piece or patch, so that photosynthesis of plants is influenced, and the leaves fall off when serious, so that the yield of growth of the papaya plants is influenced. The chemical agent is a common means for preventing and controlling the pest mites, but the long-term use of a single chemical agent can cause a series of problems such as drug resistance, pesticide residue, environmental pollution and the like, so that the development of a novel pesticide for preventing and controlling the pest mites has important significance.

Many plant-derived phenols have been studied to show a high contact killing effect on mites, such as: eugenol, carvacrol, thymol, and the like. Application number CN200910088809.3 discloses a thymol mite-killing microemulsion and a preparation method thereof, which can effectively prevent and control a plurality of pesticide mites such as tetranychus cinnabarinus, tetranychus pityriasis and tetranychus biharamous; meanwhile, the structural formula, chemical name and physicochemical property of thymol are also specifically disclosed in the patent.

The compounding of pesticide active ingredients with different action mechanisms is an effective and quick way for developing new pesticide products and preventing and controlling pest and disease resistance at present. After the different pesticide active ingredients are compounded, three action types are shown: additive action, synergistic action and antagonistic action. In general, the type of action of the different pesticide active ingredients after being compounded can be known only through a large number of experiments. The screening of the compound pesticide with synergistic effect can obviously improve the control effect, reduce the pesticide usage amount, reduce the control cost, delay the pest resistance generation and reduce the pollution to the environment, and is one of the important means of the current comprehensive control of the pests. The inventor discovers that thymol and clofentezine, ivermectin or doramectin have synergistic effect when being compounded within a certain mass ratio range through a large number of indoor biological activity tests, has great significance for developing novel pesticides, and does not see related compounding reports at present.

Disclosure of Invention

The invention aims to provide a synergistic composition for controlling papaya mites and containing thymol, which solves the problem of long-term use of a single chemical agent.

In order to achieve the above purpose, the present invention provides the following technical solutions:

the effective component of the synergistic composition is formed by binary compounding of thymol, clofentezine, ivermectin or doramectin; wherein the mass ratio of the clofentezine to the thymol is 1-5:10-1; the mass ratio of the ivermectin to the thymol is 1-10:30-1; the mass ratio of the doramectin to the thymol is 1-20:40-1.

Preferably, the mass ratio of the clofentezine to the thymol is 1:5.

preferably, the mass ratio of the ivermectin to the thymol is 1:2.

preferably, the mass ratio of the doramectin to the thymol is 5:1.

compared with the prior art, the invention has the following beneficial effects:

when two effective components in the synergistic composition are compounded in a certain mass ratio, the co-toxicity coefficient is more than 120, and the synergistic effect is shown, so that the control effect on mites can be remarkably improved, the pesticide application amount is reduced, the control cost of the mites can be reduced, and the generation of drug resistance of the mites can be delayed; on the basis, the pesticide auxiliary materials acceptable in agriculture and pharmacy are added, so that the pesticide can be prepared into chemical agents, and the pesticide has important significance in developing novel chemical agents for preventing and removing harmful mites.

Detailed Description

The following description of the embodiments of the present invention will be apparent from, and is intended to provide a thorough description of, the embodiments of the present invention, and not a complete description of, the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.

Examples: indoor activity test of thymol after being compounded

Test agent: 99% thymol technical, 95% clofentezine technical, 99% ivermectin technical and 98% doramectin technical;

test mites: tetranychus urticae, which is picked from papaya plants, is bred, propagated and hatched in a laboratory to obtain first-generation Ruo mites;

test method (refer to NT/T1154.13-2008 pesticide laboratory test guidelines pesticide section 13: leaf dish spray method)

1. Preparing a single-dose mother solution from raw medicines, setting a plurality of groups of proportions, and preparing 5 gradient mass concentrations for later use by using a 0.1% Tween-80 solution according to an equal ratio of the single-dose mother solution to each group of proportions;

2. selecting papaya leaves with consistent growth, and making the papaya leaves into leaf discs by using a puncher; placing a wet sponge block in the culture dish, placing filter paper on the wet sponge block, placing leaf discs on the filter paper, and placing 2 leaf discs on each dish; inoculating the tested mites on leaf discs, and inoculating 20 heads on each leaf disc;

3. the spray pressure of the Potter spray tower was adjusted to 1.47X 10 ⁵ Pa; placing the culture dish on a Potter spray tower chassis for spraying, wherein the spraying amount is 1mL, repeating each treatment for 4 times, and setting the treatment without the medicament as a blank control; the liquid medicine is taken out after sinking for 1min and is transferred to the temperature (25+/-1) DEG C and the photoperiod L: d= (16:8) h;

after 4.48 hours, the death condition of the tested mites is checked, the total number of insects and the death number of the mites are respectively recorded, the corrected death rate of each treatment is calculated, DPS software is used for carrying out treatment analysis on the test data, and each medicament is calculatedLC ₅₀ And the co-toxicity coefficient (CTC value) of the mixture was calculated according to the grand cloud Pei method.

P= (K/N) ×100, where P is mortality (%), K is number of dead insects, and N is total number of treated insects;

P ₁ ＝(P _t －P ₀ )/(1－P ₀ ) Wherein P is ₁ To correct mortality (%), P _t To treat mortality (%), P ₀ Mortality (%) for the blank control;

measured virulence index (ATI) = (standard agent LC ₅₀ Reagent LC ₅₀ )×100；

Theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture;

co-toxicity coefficient (CTC) = [ mix measured toxicity index (ATI)/mix Theoretical Toxicity Index (TTI) ]x100;

dividing the standard according to the joint action: the co-toxicity coefficient (CTC) is more than or equal to 120, and the synergistic effect is shown; the cotoxicity coefficient (CTC) is less than or equal to 80 and shows antagonism; 80< co-toxicity coefficient (CTC) <120 shows additive effect, and experimental results are shown in tables 1 to 3.

Table 1 indoor biological Activity assay of clomazone and thymol combinations against Tetranychus papaya

Medicament name and ratio	LC ₅₀ (mg/L)	ATI	TTI	CTC
					Clofentezine	19.32	100.00	--	--
Thymol	124.15	15.56	--	--
					Clofentezine 1: thymol 10	24.83	77.81	23.24	334.84
Clofentezine 1: thymol 8	10.37	186.31	24.94	746.90
					Clofentezine 1: thymol 5	7.23	267.22	29.63	901.71
Clofentezine 1: thymol 2	22.24	86.87	43.71	198.75
					Clofentezine1: thymol 1	18.06	106.98	57.78	185.14
Clofentezine 2: thymol 1	8.22	235.04	71.85	327.10
					Clofentezine 3: thymol 1	16.74	115.41	78.89	146.29
Clofentezine 5: thymol 1	17.31	111.61	85.93	129.89

As can be seen from table 1, the mass ratio of clofentezine to thymol is 1-5: the co-toxicity coefficients in the range of 10-1 are all larger than 120, which indicates that the biological activity of the clofentezine and thymol after being compounded on the spider mites of papaya can be shown as a synergistic effect, especially when the mass ratio is 1:5, the co-toxicity coefficient reaches 901.71, and the synergy is particularly remarkable.

Table 2 indoor bioactivity assay of ivermectin and thymol complex against spider mites of papaya

Medicament name and ratio	LC ₅₀ (mg/L)	ATI	TTI	CTC
					Ivermectin	2.63	100.00	--	--
Thymol	124.15	2.12	--	--
					Ivermectin 1: thymol 30	33.37	7.88	5.28	149.38
Ivermectin 1: thymol 20	15.03	17.50	6.78	258.11
					Ivermectin1: thymol 10	13.35	19.70	11.02	178.82
Ivermectin 1: thymol 8	6.39	41.16	12.99	316.74
					Ivermectin 1: thymol 5	4.26	61.74	18.43	334.95
Ivermectin 1: thymol 2	1.14	230.70	34.75	663.97
					Ivermectin 1: thymol 1	2.46	106.91	51.06	209.39
Ivermectin 2: thymol 1	2.72	96.69	67.37	143.52
					Ivermectin 5: thymol 1	0.86	305.81	83.69	365.43
Ivermectin 8: thymol 1	1.92	136.98	89.12	153.69
					Ivermectin 10: thymol 1	2.31	113.85	91.10	124.97

As can be seen from table 2, the mass ratio of ivermectin to thymol is 1-10: the co-toxicity coefficients in the range of 30-1 are all larger than 120, which indicates that the biological activity of the ivermectin and thymol after being compounded on the spider mites of papaya can be shown as a synergistic effect; especially in a mass ratio of 1:2, the co-toxicity coefficient reaches 663.97, and the synergy is particularly remarkable.

TABLE 3 indoor biological Activity assay of thymol and doramectin combinations against Tetranychus papaya

As can be seen from table 3, the mass ratio of doramectin to thymol is 1-20: the co-toxicity coefficients in the range of 40-1 are all larger than 120, which indicates that the biological activity of the compound doramectin and thymol on the spider mites of papaya can be shown as a synergistic effect; especially at a mass ratio of 5: when 1, the co-toxicity coefficient reaches 842.31, and the synergy is particularly remarkable.

In conclusion, the thymol and clofentezine, ivermectin or doramectin in the synergistic composition have synergistic action on the biological activity of papaya mites when being compounded in a certain mass ratio, and can effectively improve the control effect compared with a single component.

Claims

1. The synergistic composition for controlling papaya mites contains thymol, and is characterized in that the effective components of the synergistic composition are compounded by thymol and clofentezine in a binary mode; the mass ratio of the clofentezine to the thymol is 1-5:10-1.

2. The synergistic composition for controlling the harmful mites of papaya and containing thymol of claim 1, characterized in that the mass ratio of clomazone and thymol is 1:5.