CN111374135B - Compound composition of shenqinmycin and bitertanol and application thereof - Google Patents

Compound composition of shenqinmycin and bitertanol and application thereof Download PDF

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CN111374135B
CN111374135B CN202010410125.7A CN202010410125A CN111374135B CN 111374135 B CN111374135 B CN 111374135B CN 202010410125 A CN202010410125 A CN 202010410125A CN 111374135 B CN111374135 B CN 111374135B
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bitertanol
shenqinmycin
compound composition
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CN111374135A (en
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刘保友
王洪涛
王英姿
李宝燕
石洁
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Shandong Yantai Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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Abstract

The invention relates to a compound composition containing shenqinmycin and bitertanol, which is used as a bactericide for preventing and treating peanut leaf spot after being compounded, and belongs to the technical field of compound compositions of bactericides. The mass ratio of the shenqinmycin to the bitertanol in the compound composition is 1: 10-1: 20. The shenqinmycin and the bitertanol are compounded, and the optimum proportion of the compounded bactericide is screened out through indoor and outdoor tests, so that the compound bactericide has obvious synergy, is beneficial to realization, reduces environmental pollution and effectively controls the generation and development of pathogenic bacteria resistance.

Description

Compound composition of shenqinmycin and bitertanol and application thereof
Technical Field
The invention relates to a compound composition containing shenqinmycin and bitertanol, which is used as a bactericide for preventing and treating peanut leaf spot after being compounded, and belongs to the technical field of compound compositions of bactericides.
Background
Peanuts are one of important varieties of economic crops and oil crops in the world, and are also one of important export agricultural products. The healthy development of the peanut industry in China plays a significant role in increasing the income of farmers, ensuring the safety of edible oil, improving the physical health quality of the nation and the like. The leaf spot of peanut is the general term of leaf brown spot, black spot and net spot, and the diseases can occur in the same plant or even the same leaf, commonly occur in peanut producing areas, and have the tendency of increasing year by year. After the disease infects leaves, scabs from old leaves at the lower part to new leaves at the upper part are formed, and when the scabs seriously happen, the leaves fall off in advance, the photosynthesis and the fruit yield are reduced, and the yield of the damaged peanuts can be reduced by more than 20%. At present, chemical prevention and control are still used for preventing and controlling the peanut leaf spot, and with the increase of the use amount of chemical pesticides and the increase of application times, the prevention and control cost is improved, the drug resistance is caused to develop, the peanut pesticide residue is high, the environment is polluted, and the human health is indirectly influenced. The selection of the plant source bactericide and the reasonable field barrel-mixed chemical pesticide is an important way for solving the problem that the current pesticide is not scientifically and reasonably used.
The shenzine mycin (phenazino-1-carboxylic acid) is a novel microbial pesticide independently researched and created by Shanghai university of traffic Life science and technology academy and Shanghai agricultural product GmbH, has the characteristics of high efficiency, safety, broad spectrum and the like, mainly comprises a secondary metabolite phenazine-1-carboxylic acid generated by muskmelon rhizosphere growth-promoting bacteria M18, belongs to aromatic heterocyclic phenazine compounds, can effectively control fungal, bacterial and nematode diseases of crops such as various rice, wheat fruits and vegetables and the like, and simultaneously has the effect of promoting the growth of plants. However, the compounding composition of the shenqinmycin and the bitertanol has not been reported in the aspect of controlling the leaf spot of the peanut.
Biphenyl triadimenol (bitertanol) belongs to triazole bactericides and is a sterol demethylation inhibitor. The chemical name is (1RS,2RS) -1- (bis-benzene-4-oxy) -3, 3-dimethyl-1-1 (1,2, 4-triazole-1-yl) ketone-2-alcohol; molecular formula C20H23N3O2Molecular weight 337.4; the original drug is white crystal, the melting point is 118 ℃, the vapor pressure is less than 1mPa (20 ℃), and the solubility (20 ℃): 5mg/L of water, 100-200 g/L of dichloromethane and 10-30 g/L of methylbenzene. Biphenyl triadimenol is a broad-spectrum systemic fungicide and is mainly used for preventing and treating fruit tree diseases caused by Venturia and sclerotinia, and banana diseases and peanut leaf spot diseases caused by mycosphaerella. Biphenyl triazole alcohol is widely applied to planting processes of grain crops, fruits, vegetables and the like, the using amount of the biphenyl triazole alcohol is gradually increased, and food safety problems caused by residues of the biphenyl triazole alcohol are widely concerned by people. The overproof residues of the bactericide in processed products of grains, fruits and vegetables can bring great harm to the secretion system in human bodies.
Disclosure of Invention
On the basis of the prior art, the invention is completed by deeply researching the application of the compound composition of the shenqinmycin and the bitertanol in the agricultural field, in particular to the prevention and control research of the peanut leaf spot. Aims to provide the shenqinmycin and the bitertanol single agent and the compound composition thereof for preventing and treating the leaf spot of the peanut.
The invention provides a compound composition of shenqinmycin and bitertanol for preventing and treating leaf spot of peanuts, wherein the compound composition comprises the following components in parts by weight: the bitertanol has an additive effect in a ratio of 1:10, particularly has a synergistic effect in a ratio of 1:20, has synergistic coefficients of 1.80, 1.78 and 1.88 for peanut brown spots, black spots and porphyridium, and is an optimal compounding ratio.
The compound composition of the shenqinmycin and the bitertanol is characterized in that the mass ratio of the shenqinmycin to the bitertanol in the compound composition is 1: 10-1: 20;
further, the mass ratio of the shenqinmycin to the bitertanol in the compound composition is 1: 20;
further, the total mass of the shenqinmycin and the bitertanol in the compound composition accounts for 21 percent of the compound composition;
the invention also provides application of the complex composition of shenqinmycin and bitertanol, which is characterized by applying the complex composition to prevention and treatment of peanut leaf spot.
According to the complex composition of the shenqinmycin and the bitertanol, the two bactericides of the shenqinmycin and the bitertanol have good inhibition effect on alternaria alternata, and the complex application of the shenqinmycin and the bitertanol has obvious synergistic effect on preventing and treating alternaria alternata. Has the following advantages:
1. has synergistic effect. Indoor toxicity test results show that the shenqinmycin and the bitertanol have synergistic effect on the alternaria alternate in a range of 1:10 to 1:20, wherein the synergistic effect of 1:20 is most obvious, and the synergistic coefficients on the alternaria alternate, the melasma alternate and the reticulum bacteria respectively reach 1.80, 1.78 and 1.88.
2. The optimal proportion of the pesticide has prominent field tank-mixing control effect and reduces the dosage of the preparation. The composition is prepared from 21% of shenqinmycin and bitertanol60、80、100mL/667m2The compound pesticide has outstanding performance in pesticide effect tests for preventing and treating the leaf spot disease of the peanut in the field, and is superior to the prevention and treatment effects of single agents and contrast agents. Meanwhile, the dosage of the effective components of the medicament is greatly reduced.
3. High safety. The toxicity of the shenqinmycin and the bitertanol to human and livestock is low, and the content of the compound composition prepared by the hospital is lower than that of the effective component of a single agent in the market, so that the compound composition is safer to crops, environment and eaters.
4. The risk of drug resistance of pathogenic bacteria is reduced. As shown in advantages 2 and 3, the dosage of the compound composition shenqinmycin and the usage amount of the bitertanol are obviously lower than that of a single-agent product sold in the market, and the usage amount in the field is obviously reduced, so that the selection pressure of the pathogenic bacteria of the peanut leaf spot on the two bactericides can be greatly reduced. In addition, the shenqinmycin and the bitertanol have different action mechanisms, and the drug resistance of pathogenic bacteria is not easy to generate by barrel mixing.
Detailed Description
The invention is illustrated in detail by the following specific examples, which are not intended to be limiting in any way. The following description is only a preferred embodiment of the present invention, and is only for the purpose of explaining the present invention, and should not be construed as limiting the scope of the present invention. It will be apparent to those skilled in the art that various modifications, equivalent substitutions, and improvements can be made on the basis of the present invention, and any modification, equivalent substitution, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The percentages in all the proportions of the following examples are percentages by mass.
Example 1
Indoor toxicity test and compounding ratio screening
1.1 test agent
95% of raw shenqinmycin, and Shanghai Nongle biological products, product of the company Limited.
97% of bitertanol technical product, and Jiangsu Jianpai agrichemical products of GmbH.
1.2 test strains
The test targets are peanut brown spot pathogen (Cercospora arachidicola), black spot pathogen (Cercospora personata) and net spot pathogen (Phoma arachidicola).
The germ strain sample is obtained by separating from field disease leaves, culturing and purifying to obtain the first generation of germ strain, and storing in a refrigerator at 4 ℃ for later use.
1.3 instrumentation
Mainly comprises an electronic balance, an ultra-clean workbench, a biological incubator, a culture dish, a micropipette and the like.
1.4 Experimental methods
The FAO-recommended cell growth rate method (Mycelium growth rate test) was used.
1.4.1 compounding of medicaments
TABLE 1 compounding ratio of shenqinmycin and bitertanol
Figure DEST_PATH_IMAGE001
TABLE 2 dilution concentration gradient of the compounded agents
Figure DEST_PATH_IMAGE002
1.4.2 preparation of drug-containing plates
Adding the medicine into PDA culture medium dissolved quantitatively according to the medicine concentration gradient set at 1.4.1 to obtain a medicine-containing plate, and repeating for 3 times each concentration with the plate without medicine as blank control.
1.4.3 inoculum culture
And (3) punching a bacterium disc along the edge of the cultured bacterial colony by using a puncher with the diameter of 0.6cm, reversely transplanting the bacterium disc onto a flat plate by using a disinfection inoculating needle, and culturing at the constant temperature of 25 ℃ for 5 days.
1.4.4 investigation of results
And measuring the expansion diameter of the bacterial colony by a ruler cross measurement method, and calculating the inhibition rate of the medicament on the growth of hyphae compared with the expansion diameter of a control bacterial disc without applying the medicament.
Figure BDA0002492858440000041
1.5 statistical analysis
Regression analysis is carried out according to the logarithm value of the concentration of each medicament and the corresponding hyphal growth inhibition rate value, and a toxicity regression equation, a correlation coefficient (r) and EC of each medicament are calculated50。
And (3) when the combined toxicity of the medicaments is measured, calculating the synergistic coefficient (SR) of the compound composition according to the Wadley method, and evaluating the combined action type of the compound composition. The synergistic effect of the compounded medicament is evaluated according to the synergistic coefficient (SR), namely, SR <0.5 is antagonistic action, SR <0.5 and less than or equal to 1.5 are additive action, and SR >1.5 is synergistic action. The synergy coefficient (SR) is calculated according to the formulas (1) and (2):
Figure BDA0002492858440000042
in the formula:
X1- - -built-up composition EC50Theoretical values in mg/L;
PAthe percentage of a in the compounded composition is given in percent (%);
PBthe percentage of B in the compounded composition, in percent (%);
EC of A- - - -A50Values in mg/L;
EC of B- - - -B50Values in mg/L.
Figure BDA0002492858440000043
In the formula:
SR- - -synergistic coefficient of the compounded composition;
X1- - -built-up composition EC50Theoretical values in mg/L;
X2- - -built-up composition EC50Found in mg/L.
1.6 analysis of results
The indoor optimal compounding ratio experiment result shows that 1:10 and 1:20 of both the shenqinmycin and the bitertanol have synergistic effect on peanut brown spots, black spots and dictyosphaera, wherein the synergistic effect of 1:20 is most obvious, and the synergistic coefficients are 1.80, 1.80 and 1.88 respectively, which are the optimal compounding ratio.
TABLE 3 screening of the optimal compounding ratio of shenqinmycin and bitertanol for inhibiting peanut brown spot pathogen
Figure DEST_PATH_IMAGE003
TABLE 4 screening of the optimal compounding ratio of shenqinmycin and bitertanol for inhibiting peanut alternaria alternata
Figure DEST_PATH_IMAGE004
TABLE 5 screening of the optimal compounding ratio of shenqinmycin and bitertanol for inhibiting peanut latticed bacteria
Figure DEST_PATH_IMAGE005
Example 2
Plot experiment in the field
According to an indoor compounding ratio screening experiment, the optimum ratio of the shenqinmycin to the bitertanol is screened out, and a 21% compound composition of the shenqinmycin and the bitertanol is prepared. The field efficacy test of the 21% shenqinmycin-bitertanol compound composition on the mosaic leaf spot is designed according to 85 th part of pesticide field efficacy test criteria (II) mainly compiled by pesticide verification of Ministry of agriculture.
2.1 test Agents
100mL/667m of 1% shenqinmycin suspension2Shanghai agricultural Happy biological products, Inc.
25% bitertanol wettable powder 66g/667m2Jiangsu Jian Nonghua GmbH;
21% shenqinmycin-bitertanol 60, 80, 100mL/667m2Prepared by a tobacco platform farm institute.
2.2 control Agents
500g/L carbendazim suspending agent 100m/667m2The product of Jiangsu Longdeng chemical Co.
2.3 cell design
The experiment had 7 processes, 4 replicates each, 28 cells, 50m each2. The cells are randomly arranged. The pesticide application device adopts MATABI SuperGreen 16 type knapsack sprayer, the diameter of the spray hole is 0.8mm, and the working pressure is 2.0 bar. When spraying, the front and back sides of the leaf are uniformly coated with the medicine until dripping.
2.4 methods of investigation
Level 0: no disease;
level 1: the area of the lesion spots accounts for less than 5% of the area of the whole leaf;
and 3, level: the area of the lesion spots accounts for 6 to 25 percent of the area of the whole leaf;
and 5, stage: the area of the lesion spots accounts for 26-50% of the area of the whole leaf;
and 7, stage: the area of the lesion spots accounts for 51 to 75 percent of the area of the whole leaf;
and 9, stage: the area of the lesion spots accounts for more than 76% of the area of the whole leaf.
2.5 method of calculating drug efficacy
Figure BDA0002492858440000061
Figure BDA0002492858440000062
Figure BDA0002492858440000063
2.6 test results
21% shenqinmycin-bitertanol compound composition 60, 80 and 100mg/667m2The control effects are respectively 90.80%, 92.40% and 94.41%, which are obviously higher than 100mL/667m of 1% shenqinmycin suspending agent2And 25% bitertanol wettable powder 66mL/667m2The prevention and the treatment effect are improved; single agent and compound preparationThe control effect of the preparation is obviously better than that of a contrast medicament 500g/L carbendazim suspending agent 100mL/667m2The prevention and treatment effect of the composition is good.
Field pesticide effect of table 421% shenqinmycin-bitertanol compound composition for preventing and treating peanut leaf spot disease
Figure DEST_PATH_IMAGE006
Example 3
Safety test
The content of the shenqinmycin and the content of the bitertanol in the compound composition are less than that of products sold in the market, so that the toxicity to human and livestock and the safety of crops are in accordance with requirements. The toxicity test result of the compound bactericide by a affirming organization shows that the compound bactericide belongs to a slightly toxic pesticide:
rat acute oral toxicity LD50 of more than 5000mg/kg and male parent of more than 5000 mg/kg;
rat acute percutaneous toxicity LD50 is more than 20000mg/kg in female parent and more than 20000mg/kg in male parent.

Claims (2)

1. The application of the compound composition of the shenqinmycin and the bitertanol in preventing and treating the leaf spot of peanuts is characterized in that the mass ratio of the shenqinmycin to the bitertanol in the compound composition is 1: 20.
2. The application of the complex composition of shenqinmycin and bitertanol as claimed in claim 1 in preventing and treating peanut leaf spot disease, wherein the total mass of the shenqinmycin and the bitertanol in the complex composition accounts for 21% of the complex composition.
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