CN100418934C - Disease-resisting fertilizer capable of resisting disease and providing nutrient to plant - Google Patents

Disease-resisting fertilizer capable of resisting disease and providing nutrient to plant Download PDF

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Publication number
CN100418934C
CN100418934C CNB2005100486453A CN200510048645A CN100418934C CN 100418934 C CN100418934 C CN 100418934C CN B2005100486453 A CNB2005100486453 A CN B2005100486453A CN 200510048645 A CN200510048645 A CN 200510048645A CN 100418934 C CN100418934 C CN 100418934C
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disease
fertilizer
resistant
arasaponin
nitrate
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CN1970509A (en
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潘艳华
曾莉
张晓林
郭玉蓉
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Yunnan Academy of Tobacco Agricultural Sciences
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Yunnan Academy of Tobacco Agricultural Sciences
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Abstract

The invention discloses a disease-resistant fertilizer, which is characterized by the following: setting the rate of cupric nitrate, zinc nitrate and notoginseng escin at 1:5:0.1-0.5; protecting plant from powdery mildew, late blight, downy mildew, macrophyric disease and aphid without pollution or toxicity; providing nourishment and microelement for crop; accelerating the growth of crop; improving crop quality.

Description

A kind of not only disease-resistant but also the disease-resistant fertilizer of plant nutrition can be provided
Technical field
The present invention relates to a kind of fertilizer, the disease-resistant fertilizer of part plant nutrient ingredient can also be provided in disease-resistant with resistant effect.
Background technology
Diseases and pests of agronomic crop is one of important factor that directly influences agriculture production and farmers' income; at present, the means of control corps diseases mainly contain: agricultural measures (comprise the disease-resistant worm kind of seed selection, efficent rotation, adjustment sowing time etc.), biological control measure (with worm control worm, microorganism is controlled worm, microbiotic and cross-protection), physical control (with physical method prevention and elimination of disease and pests such as temperature, light, hertzian wave, ultrasonic wave, nuclear radiation), chemical prevention (applied chemistry pesticide control disease and pest).Chemical prevention for a long time is the main path of control diseases and pests of agronomic crop always, and other prevents and treats method all owing to such-and-such limitation is arranged, and can't replace chemical prevention fully.And the long-term application chemical pesticide easily causes pesticide residue, environmental pollution and disease and pest resistance etc.Improving constantly particularly along with living standards of the people, food-safety problem has caused people's attention, and is increasing to the demand of green and Organic food, selects efficient, low toxicity, less-persistent pesticide, reduce pesticide residue and, become an inevitable trend the pollution of product and environment.Aspect fertilizer uses, owing to use chemical fertilizer in a large number for a long time, think little of organic fertilizer application in recent years, make Soil structure become bad, reduced utilization rate of fertilizer, nutrient loss such as nitrogen phosphorus also cause environmental pollution.
Some specific fertilizer is handled, made it when using, can provide crop certain nutritive ingredient, promote plant growth, and can reach the effect of control disease and pest, and strengthen plant resistance to environment stress, improve prevention effect, reduce the chemical pesticide amount of application, improve product quality.Therefore, development can provide nutritive ingredient to plant-growth, simultaneously again can diseases prevention, disease-resistant disease-resistant fertilizer, have necessity and feasibility meaning.
Summary of the invention
The present invention has proposed a kind of not only disease-resistant for the deficiency that solves prior art existences just but also the disease-resistant fertilizer of plant nutrition can be provided.
The present invention realizes by following technical proposal: a kind of not only disease-resistant but also the disease-resistant fertilizer of plant nutrition can be provided, it is characterized in that forming: cupric nitrate: zinc nitrate: arasaponin=1: 5: 0.1~0.5 by following proportion of masses.
Provided by the invention not only disease-resistant but also can provide the disease-resistant fertilizer of plant nutrition to be made into liquid fertilizer: cupric nitrate: zinc nitrate: arasaponin: water=1: 5: 0.1~0.5: 100 by following mass ratio.
Provided by the invention not only disease-resistant but also can provide the disease-resistant fertilizer of plant nutrition to be made into solid fertilizer: cupric nitrate: zinc nitrate: arasaponin: diatomite=1: 5: 0.1~0.5: 10~20 by following mass ratio.
Described cupric nitrate, zinc nitrate are commercially available purity at 98% industrial cupric nitrate and zinc nitrate, and arasaponin is the commercially available prod.
Described diatomite is for containing SiO 240%~70%, contain the product that organic matter 20%~30% originates in Yunnan Xun Dian.
Cupric nitrate, zinc nitrate in the present invention's prescription; its nitrate ion is one of principal mode that provides to plant nitrogen nutrition; copper, zine ion itself have certain sterilizing power; after allocating arasaponin in the prescription into pharmacologic action; making it not only has stronger provide protection to plant, and can effectively improve the resistance against diseases of plant.Allocating organic content in solid fertilizer into reaches more than 20%, and after having the strong diatomite of exquisiteness, loose, light weight, porous, water-absorbent and perviousness, can not only solve cupric nitrate, the aerial sucting wet problem of zinc nitrate, prolong the storage life, and can also improve soil property, promote the crop healthy growth, improve crop quality.In prescription, directly add water and be made into liquid fertilizer and directly spray application to plant surface, can diseases prevention, disease-resistant, can partly replenish the required nutritive ingredient of plant-growth again.
The present invention makes by following method: 1, with standby behind cupric nitrate, zinc nitrate and the abundant mixing of powdery arasaponin; 2, diatomite was pulverized 0.25mm sieve after, it is sneaked in above-mentioned cupric nitrate, zinc nitrate and the arasaponin mixture, fully promptly get solid fertilizer behind the mixing, execute as soil; Perhaps, 3, with cupric nitrate, zinc nitrate and arasaponin mixture with water dissolution after, promptly get liquid fertilizer.
The present invention compared with prior art has following advantage and effect: the disease-resistant fertilizer that adopts such scheme to be made into; plant there is stronger provide protection; resistance against diseases is better than single variety; can effectively prevent and prevent and treat the crop red-star like disease; Powdery Mildew; late blight; oidium; leaf blight and aphid etc.; to farm crop; environment does not pollute; non-residual toxicity; preventing and kill off diseases and pests of agronomic crop simultaneously; can provide nutrition to crop; the deficiency of trace element supplement copper zinc; adding diatomaceous soil executes solid fertilizer and can also improve soil property; promote the sound growth of crop; improve crop quality; can supply corn; paddy rice; wheat; farm crop such as barley; tobacco; rape; cash crop such as flax; tomato; string bean; greengrocery crops such as potato; citrus; grape; apple; fruit trees such as cherry are used, especially for preventing and kill off the crop red-star like disease; Powdery Mildew and aphid etc. have better effect.
Embodiment
Below in conjunction with embodiment the present invention is described further.
Embodiment 1
Take by weighing 98% 5 kilograms of 1 kilogram of industrial cupric nitrate, zinc nitrates, 0.2 kilogram of powdery arasaponin by weight, fully mixing.Diatomite was pulverized the 0.25mm sieve for 10 kilograms, afterwards the copper that stirs, zinc, arasaponin mixture was added in the diatomite, and mixing and stirring gets solid fertilizer and executes as soil.
Embodiment 2
Take by weighing 98% 5 kilograms of 1 kilogram of industrial cupric nitrate, zinc nitrates, 0.3 kilogram of powdery arasaponin by weight,, be made into the liquid fertilizer that 100 kg of water solution promptly get foliage-spray respectively copper zinc mixture and arasaponin dissolved in distilled water.
Embodiment 3
Take by weighing 98% 5 kilograms of 1 kilogram of industrial cupric nitrate, zinc nitrates, 0.5 kilogram of powdery arasaponin by weight, fully mixing.Diatomite was pulverized the 0.25mm sieve for 20 kilograms, afterwards the copper that stirs, zinc, arasaponin mixture was added in the diatomite, and mixing and stirring gets solid fertilizer and executes as soil.
Embodiment 4
Take by weighing 98% 5 kilograms of 1 kilogram of industrial cupric nitrate, zinc nitrates, 0.1 kilogram of powdery arasaponin by weight, fully mixing.Diatomite was pulverized the 0.25mm sieve for 15 kilograms, afterwards the copper that stirs, zinc, arasaponin mixture was added in the diatomite, and mixing and stirring gets solid fertilizer and executes as soil.
Embodiment 5
Take by weighing 98% 5 kilograms of 1 kilogram of industrial cupric nitrate, zinc nitrates, 0.5 kilogram of powdery arasaponin by weight,, be made into the liquid fertilizer that 100 kg of water solution promptly get foliage-spray respectively copper zinc mixture and arasaponin dissolved in distilled water.
Embodiment 6
Take by weighing 98% 5 kilograms of 1 kilogram of industrial cupric nitrate, zinc nitrates, 0.1 kilogram of powdery arasaponin by weight,, be made into the liquid fertilizer that 100 kg of water solution promptly get foliage-spray respectively copper zinc mixture and arasaponin dissolved in distilled water.
For showing the present invention's effect, through following application example, its result is as follows:
One, disease-resistant fertilizer is tested the tobacco brown spot pathogen spore germination
1 test objective
The disease-resistant fertilizer of being prepared is carried out the indoor bactericidal activity toxicity test, for the Application and Development of disease-resistant fertilizer provides foundation.
2 test materialss
2.1 test medicine
Reagent agent: disease-resistant fertile No. 1 (cupric nitrate: zinc nitrate=1: 5)
Disease-resistant fertile No. 2 (cupric nitrates: zinc nitrate: arasaponin=1: 5: 0.2)
Disease-resistant fertile No. 3 (cupric nitrates: zinc nitrate: arasaponin: diatomite=1: 5: 0.2: 10)
Disease-resistant fertile No. 4 (cupric nitrates: zinc nitrate: diatomite=1: 5: 10)
Prepare by agricultural environment institute of Yunnan Academy of Agricultural Sciences.
Contrast medicament: 40% dimetachlone (WP) (developing jointly) by Lucheng City, Wenzhou City, Zhejiang Province plant protection laboratory, oil plant institute of the Chinese Academy of Agricultural Sciences, the petrochemical industry Room, Zhejiang Province fertilizer and pesticide company
Blank: clear water.
2.2 target organisms: Alternaria alternate [Alternaria alternata (Fries) keissler].
2.3 test site: agricultural environment institute of Yunnan Academy of Agricultural Sciences.
3 test methods and step
3.1 the preparation of reagent agent
1. 1~4 disease-resistant fertilizer is prepared the back dissolution filter, and constant volume to 500 times medicament uses.
2. contrast 1:40% dimetachlone (WP) 200.00mg/L
3. contrast the 2:(clear water) etc. totally 6 processing.
3.2 the preparation of spore suspension
An amount of water of brown spot pathogen adding of spore is produced in pure culture on flat board, brushes down the spore on bacterium colony surface gently with writing brush, and with 4 layers of filtered through gauze, the dilution spore is the spore liquid of about 100 spores in every visual field under 100 times of visuals field.
3.3 the making of germinating bed
Get clean test tube, adds the above-mentioned soup that has prepared 40 microlitres and 40 microlitre spore suspensions and mix and drip on the depression slide of cleaning every processing 6 repetitions.Depression slide is placed in the culture dish of preserving moisture, put into 28 ℃ of incubators and cultivate.When control group spore germ tube length be spore 1/2 the time, counting is respectively handled the sprouting number of spore, calculates each correction of handling and suppresses spore germination rate.
3.4 the result calculates
Figure C20051004864500071
4, test-results
Classify test-results as table 1.
The disease-resistant fertilizer of table 1 is to the spore germination test-results of tobacco brown spot pathogen
Figure C20051004864500073
5 conclusions
Show by shop experiment, disease-resistant fertile No. 1, No. 2, No. 3, No. 4 tobacco brown spot pathogen had certain virulence, especially No. 2 and No. 3 fertilizer, the spore germination test-results, it is proofreaied and correct the spore germination inhibiting rate and all reaches 100%, No. 1 and No. 4 are poor slightly, but have all surpassed the contrast chemicals treatment, reach about 90%.So several prescriptions all have good inhibitory effect to tobacco brown spot pathogen, but the treatment effect optimum of adding arasaponin.
Two, disease-resistant fertilizer prevention effect to red-star like disease on the tobacco excised leaf is tested
1 test objective
The disease-resistant fertilizer of being prepared is carried out prevention effect test on the tobacco excised leaf, for the Application and Development of disease-resistant fertilizer provides foundation.
2 test materialss
2.1 test medicine
(1) reagent agent: disease-resistant fertile No. 1~No. 4 (by the preparation of agricultural environment institute of Yunnan Academy of Agricultural Sciences).
(2) contrast medicament: 500 times of liquid of 40% dimetachlone (WP) (developing jointly) by Lucheng City, Wenzhou City, Zhejiang Province plant protection laboratory, oil plant institute of the Chinese Academy of Agricultural Sciences, the petrochemical industry Room, Zhejiang Province fertilizer and pesticide company.
(3) blank: clear water.
2.2 pathogenic bacteria: tobacco brown spot pathogen [Alternaria alternata (Fries) keissler].
2.3 tobacco bred: K326.
2.4 test site: agricultural environment institute of Yunnan Academy of Agricultural Sciences.
3 test methods and step
3.1 the preparation of reagent agent
1. disease-resistant fertile No. 1~No. 4, a same test of compound method.
2. contrast 500 times of liquid of 1: 40% dimetachlone (WP);
3. contrast the 2:(clear water) etc. totally 6 processing.
3.2 the preparation of spore suspension
An amount of water of brown spot pathogen adding of spore is produced in pure culture on flat board, brushes down the spore on bacterium colony surface gently with writing brush, and with 4 layers of filtered through gauze, the dilution spore is the spore liquid of about 100 spores in every visual field under 100 times of visuals field.
3.3 for using of reagent
Gather leaf age and the uniform tobacco leaf of growing way, be cut into the blade of 5cm * 5cm with scissors, immerse above-mentioned 6 kinds in will above-mentioned 6 kinds of confession reagent in the reagent, soak whole blade face after, press from both sides out with tweezers, dry, place the vinyl disc that is covered with wet towel; Then, inoculate brown spot pathogen with transfer pipet drop spore suspension, and preserve moisture with cotton pellet; With after the vinyl disc mouth sealing, place 28 ℃ of incubators to cultivate with preservative film.Every processing 3 repeats 5 small pieces leaf/repetition.After 5 days, check incidence, investigate the disease severity of getting a haircut everywhere, calculate prevention effect at last.
3.4 the result calculates
Sickness rate (%)=morbidity bacterium point * 100 of counting/always connect
Disease index=∑ (scabs at different levels * relative level numerical value)/(investigating total scab number * 7) * 100
Prevention effect (%)=(disease index after CK disease index-dispenser)/CK disease index * 100
4 test-results
Test-results is classified table 1 as.
The disease-resistant fertilizer of table 1 is prevented and treated test-results to red-star like disease on the tobacco excised leaf
5 conclusions
Show by the test of tobacco excised leaf, disease-resistant fertile No. 1, No. 2 and No. 3 have the better prevention effect to Alternaria alternate, and wherein the prevention effect of No. 2 disease-resistant fertilizer is best, has reached 100.00%, next is No. 3 and No. 1 disease-resistant fertilizer, and prevention effect has reached 95.12% and 87.81% respectively; The preventive effect of these three kinds of disease-resistant fertilizer has all surpassed 500 times of liquid of contrast medicament 40% dimetachlone.But the prevention effect of No. 4 disease-resistant fertilizer only is 68.29%, and comparison is low according to medicament.
Three, disease-resistant fertilizer effect on corn
1 test objective
1~No. 4 disease-resistant fertilizer of being prepared is carried out regular plot experiment between corn field, verify it, for the application of disease-resistant fertilizer provides foundation to disease resisting effect of corn and to the influence of output.
2 tests are implemented
2.1 test site basic condition
Test is located at the village of ascending a height, Baoshan City Longyang District Dao Jie township, Yunnan Province, and householder sieve is gentle and quiet.Previous crops soya bean, soil are alluviation red soil.700 meters of height above sea level, 21.3 ℃ of average annual temperature, 747.6 millimeters of average annual rainfalls, sunshine time 2318.1 hours, a year frostless number of days is 350 days.
2.2 test is handled and is implemented
Test is 5 processing, 3 multiple plot experiments, and processing and performance are as follows:
Handle 1:CK
Handle disease-resistant fertilizer (2kg/667m 2:CK+1 number 2Soil is executed+2kg/667m 2Spray)
Handle disease-resistant fertilizer (2kg/667m 3:CK+2 number 2Soil is executed+2kg/667m 2Spray)
Handle disease-resistant fertilizer (10kg/667m 4:CK+3 number 2Soil is executed)
Handle disease-resistant fertilizer (10kg/667m 5:CK+4 number 2Soil is executed)
8.6 meters of zone leaders, wide 3.2 meters, 27.5 square metres of sub-district areas.
Each is handled disease-resistant loam and executes part and evenly apply at seeding time in the seed furrow, with the abundant mixing of soil and cover thin soil, avoids contacting seed.Spray the part the corn typhon mouth phase by every 667m 2Convert water 100kg foliage-spray, CK and two processing that do not spray replace with the clear water that sprays equivalent.
CK rate of fertilizer application and mode: fertilizer 100kg/667m 2, base imposes in the seed furrow or the sowing bonnet pool (ditch).Purity nitrogen amount of application 18kg/667m 2, N: P 2O 5: K 2O=is 1: 0.3: 0.3, and fertilization mode sees Table 1.
Table 1 is handled 2~4 chemical fertilizer application modes
Use period Nitrogenous fertilizer % Phosphate fertilizer % Potash fertilizer % Farm manure %
Base fertilizer 20 40 30 100
Jointing fertilizer 50 60 70
Granulated fertilizer 30
For the examination corn variety is No. six, three Norths.Test in whole ground on April 20th, 2005, spread fertilizer over the fields fertilizer 1000kg/667m2 during whole ground and make base fertilizer, sowing on April 28, carry out the width double rows line seeding, 100 centimetres of wide row, 60 centimetres of narrow rows, 22~25 centimetres of spacing in the rows, every sub-district kind 4 row, thickness of sowing is 4000 strains/667m2.May 6, neat seedling stayed by force a little less than going behind the neat seedling, about 667m2 final singling 3600 strains.Executed jointing fertilizer May 28, July 3 granules application fertilizer, August 10 results.
3 test-results
3.1 disease and pest takes place and prevents and treats situation
Secondly be aphid, be snout moth's larva in the main harm insect pest of full phase of corn growth; Disease is mainly leaf blight and rust, and usefulness Le Siben, farmland are found pleasure in, thiophanate methyl is is respectively prevented and treated once.Below be respectively to handle the susceptible rate investigation result of different times plant leaf.
Table 2 is respectively handled the susceptible situation of main plant breeding time
The investigation date Handle 1 Handle 2 Handle 3 Handle 4 Handle 5
May 30 3.5% 2.3% 2.1% 1.9% 2.0%
June 20 9.0% 7.5% 6.3% 6.5% 7.9%
July 10 16.4% 13.5% 11.8% 12.1% 13.8%
Add up to 28.9% 23.3% 20.2% 20.5% 23.7%
As can be seen from the above table, in whole growing, the susceptible rate of plant is all the highest with contrast, and all the other are followed successively by handles 5,2,4,3.Wherein, 5 processing are approaching with the susceptible rate of 2 processing; Processing 3 is approaching and minimum with the 4 susceptible rates of processing, illustrates that the disease-resistant fertile fungistatic effect that adds arasaponin is best, and the susceptible rate average specific of corn contrasts low 8 percentage points, does not add arasaponin though handle 3,4, and the susceptible rate of plant is also compared and shone low about 5 percentage points.
3.2 corn yield is influenced
Reality is carried out in each sub-district receive the survey product, get yield data and see Table 2.
Table 2 is respectively handled yield result list position: kg
Figure C20051004864500111
As can be seen from the above table, each is handled corn yield and all compares according to volume increase, amount of increase in production from 5.2% to 9.1%.Be to handle 3 volume increase to be 9.1% to the maximum, next is for processing 4 and handle 5, and handling 2 volume increase less is 5.2%.Variance analysis is carried out in each processing, and it is not remarkable to handle differences.
4 brief summaries
1~No. 4 disease-resistant fertilizer all has good inhibitory effect to maize diseases, and adds the disease-resistant fertilizer of arasaponin, and disease resisting effect strengthens than the prescription that does not add arasaponin, illustrates that arasaponin has the effect that strengthens copper, zinc resistance against diseases.
Though it is not remarkable to the corn yield increasing effect to use disease-resistant fertilizer, volume increase all reaches more than 5%, and production-increasing function is good with the disease-resistant fertilizer that adds arasaponin.
The plot experiment presentation of results, arasaponin is used as additive and copper zinc, can strengthen the crop disease-resistant ability, increase output, can strengthen under situation with good conditionsi and be applicable.

Claims (5)

  1. One kind not only disease-resistant but also the disease-resistant fertilizer of plant nutrition can be provided, it is characterized in that forming: cupric nitrate: zinc nitrate: arasaponin=1: 5: 0.1~0.5 by following proportion of masses.
  2. 2. disease-resistant fertilizer according to claim 1 is characterized in that described disease-resistant fertilizer is made into liquid fertilizer by following mass ratio: cupric nitrate: zinc nitrate: arasaponin: water=1: 5: 0.1~0.5: 100.
  3. 3. disease-resistant fertilizer according to claim 1 is characterized in that described disease-resistant fertilizer is made into solid fertilizer by following mass ratio: cupric nitrate: zinc nitrate: arasaponin: diatomite=1: 5: 0.1~0.5: 10~20.
  4. 4. disease-resistant fertilizer according to claim 1 is characterized in that described cupric nitrate, zinc nitrate are commercially available purity at 98% industrial cupric nitrate and zinc nitrate, and arasaponin is the commercially available prod.
  5. 5. disease-resistant fertilizer according to claim 3 is characterized in that described diatomite for containing SiO240%~70%, contains organic 20%~30% product.
CNB2005100486453A 2005-11-24 2005-11-24 Disease-resisting fertilizer capable of resisting disease and providing nutrient to plant Expired - Fee Related CN100418934C (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105541492A (en) * 2016-01-26 2016-05-04 安徽省司尔特肥业股份有限公司 Biological compound fertilizer for preventing potato late blight
CN106070387A (en) * 2016-06-30 2016-11-09 山东胜伟园林科技有限公司 A kind of anti-salt alkali composition containing Radix Notoginseng and preparation method thereof
CN113336602A (en) * 2021-07-28 2021-09-03 广西易邦科技有限公司 Novel fertilizer containing lysozyme

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04316507A (en) * 1991-04-16 1992-11-06 Nikko Kyodo Co Ltd Plant viral disease-controlling agent
KR20030021750A (en) * 2001-09-07 2003-03-15 조재근 Organic germanium fertilizer and method for producing the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04316507A (en) * 1991-04-16 1992-11-06 Nikko Kyodo Co Ltd Plant viral disease-controlling agent
KR20030021750A (en) * 2001-09-07 2003-03-15 조재근 Organic germanium fertilizer and method for producing the same

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