CN112438259A - Bactericide composition for preventing and treating white disease of foxtail millet - Google Patents
Bactericide composition for preventing and treating white disease of foxtail millet Download PDFInfo
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- CN112438259A CN112438259A CN202011398402.3A CN202011398402A CN112438259A CN 112438259 A CN112438259 A CN 112438259A CN 202011398402 A CN202011398402 A CN 202011398402A CN 112438259 A CN112438259 A CN 112438259A
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- Prior art keywords
- carvacrol
- kresoxim
- methyl
- preventing
- millet
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating the white disease of foxtail millet. The active ingredients of the bactericide composition for preventing and treating the white disease of the foxtail millet are formed by binary compounding of carvacrol, kresoxim-methyl, benzovindiflupyr or mandipropamid. After the effective components are compounded, 2 effective components have synergistic effect, so that the synergistic effect is remarkable, the prevention and treatment effect on the white disease of millet can be improved, the application dosage of pesticide can be reduced, the pesticide residue can be reduced, and the environmental pressure can be reduced. According to the invention, carvacrol and kresoxim-methyl, benzovindiflupyr or mandipropamid are subjected to binary compounding, so that the risk of drug resistance can be reduced when the compound is used, and the defect of single application of any effective component can be effectively overcome.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating the white disease of foxtail millet.
Background
For farmers who plant foxtail millet, the disease of foxtail millet is a common disease, and the pathogenic bacteria of the disease is foxtail millet. Pathogenic bacteria of the millet whitefly disease are mixed in manure and soil as oospores or adhered to the surface of seeds for overwintering, wherein soil-borne bacteria are main overwintering bacteria sources and can survive in the soil for 2-3 years generally. The later stage tissue of the diseased plant is cracked, the oospore overwinter on the soil, the diseased residual body and the manure seed, and the infection cycle is completed. The onset of the white millet disease shows different symptoms at different stages of the millet growth, which seriously affects the survival of millet seedlings and the heading of the millet, and further affects the yield of the millet.
Carvacrol is a pure botanical fungicide which is extracted and processed from a yellow flower bud, has a strong antibacterial effect, and can effectively inhibit the growth of pathogenic bacteria spores and hyphae; CN201510084511.0 discloses a bactericidal composition containing penflufen and carvacrol and application thereof, in particular to a bactericidal composition which has good control effect on powdery mildew or rust disease of wheat, fruit trees, flowers and vegetables after the penflufen and the carvacrol are compounded. A large number of indoor biological activity tests of the inventor show that after carvacrol and kresoxim-methyl, benzovindiflupyr or mandipropamid are compounded according to a certain proportion, the biological activity of the compound for the disease of the millet white shows a synergistic effect, and no related report about the compounding of carvacrol and kresoxim-methyl, benzovindiflupyr or mandipropamid exists at present.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a bactericide composition for preventing and treating the white disease of foxtail millet, which solves the problem caused by single application of one chemical agent.
In order to achieve the purpose, the invention provides the following technical scheme:
the active ingredients of the bactericide composition for preventing and treating the white disease of the foxtail millet are formed by binary compounding of carvacrol, kresoxim-methyl, benzovindiflupyr or mandipropamid.
Preferably, the mass ratio of carvacrol to kresoxim-methyl is 1-20: 40-1.
Preferably, the mass ratio of carvacrol to benzovindiflupyr is 1-60: 40-1.
Preferably, the mass ratio of carvacrol to mandipropamid is 1-20: 20-1.
Compared with the prior art, the invention has the following beneficial effects:
(1) after the active ingredients are compounded, 2 active ingredients have a synergistic effect, so that the synergistic effect is remarkable, the prevention and treatment effect on the white disease of millet can be improved, the application dosage of pesticide can be reduced, the pesticide residue can be reduced, and the environmental pressure can be reduced.
(2) According to the invention, carvacrol and kresoxim-methyl, benzovindiflupyr or mandipropamid are subjected to binary compounding, so that the risk of drug resistance can be reduced when the compound is used, and the defect of single application of any effective component can be effectively overcome.
Detailed Description
The technical solutions of the present invention are described in detail below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples: indoor bioactivity test after carvacrol compounding
Reagent to be tested: 83% of carvacrol technical, 96% of kresoxim-methyl technical, 96% of benzovindiflupyr technical and 92% of mandipropamid technical (Xiongda Nantong crop protection Co., Ltd.);
test subjects: mycelia collected from field foxtail millet white hair germ (Sclerospora graminicola (Sacc.) Schroeter) were isolated and purified in a laboratory, and cultured with a medium for use;
the test method comprises the following steps: (refer to NY/T1156.2-2006 "laboratory bioassay Standard for agricultural chemicals Fungicide part 2: inhibition of pathogenic fungi hypha growth test plate method")
1. Preparing a medicament: dissolving the raw materials with methanol, diluting with 0.1% Tween-80 solution, and setting the series mass concentration;
2. medicament treatment: under aseptic conditions, 10mL of the sterilized PDA culture medium which is melted in advance is added into an aseptic conical flask, 1mL of liquid medicine is quantitatively absorbed from low concentration to high concentration in sequence, and the liquid medicine is respectively added into the conical flasks and fully shaken. Then, the mixture was poured into 4 dishes having a diameter of 10cm in equal amounts to prepare drug-containing plates, and blank controls containing methanol and 0.1% Tween-80 were added to the plates, and 3 treatments were repeated for each treatment.
3. Inoculating and culturing: cutting a bacterial cake with the diameter of 5mm from the edge of a bacterial colony by using a sterilization puncher with the diameter of 5mm under the aseptic condition; inoculating the fungus cake in the center of the medicated plate with the hypha facing upwards, covering with a dish, and culturing at 25 deg.C in an incubator.
4. Investigation and calculation: when the diameter of the blank control colony is more than 2/3 of the diameter of the culture dish, measuring the diameter (unit: mm) of the colony by a caliper, vertically measuring the diameter of each colony by a cross method once, taking the average value, calculating the hypha growth inhibition rate of each treatment, performing regression analysis according to the concentration logarithm value of each medicament and the corresponding hypha growth inhibition rate value, calculating the EC50 of the medicament, and calculating the cotoxicity coefficient (CTC value) of the mixture according to the Tanbei method.
The calculation method comprises the following steps:
colony growth diameter-colony diameter-cake diameter (in this example, cake diameter is 5 mm);
the hypha growth inhibition ratio (%) [ (blank control colony growth diameter-medicament-treated colony growth diameter) ÷ blank control colony growth diameter ] × 100;
measured virulence index (ATI) ═ (standard agent EC50 ÷ test agent EC50) × 100;
theoretical virulence index (TTI) ═ a agent virulence index × percentage of a in the mixture + B agent virulence index × percentage of B in the mixture;
co-toxicity coefficient (CTC) × 100 [ actually measured toxicity index (ATI) ÷ theoretical toxicity index of the mixture (TTI) ].
Criteria are divided according to joint action:
the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80< co-toxicity coefficient (CTC) <120 showed additive effect.
The results are shown in tables 1-3.
TABLE 1 determination of the indoor biological Activity of the Compound Cochinospermum bigeminum of carvacrol and Phenylkresoxim-methyl
| Name and proportion of the medicament | EC50(mg/L) | ATI | TTI | CTC |
| Carvacrol | 1.64 | 100.00 | —— | —— |
| Benzene kresoxim-methyl | 5.86 | 27.99 | —— | —— |
| Carvacrol 1: kresoxim-methyl 40 | 3.58 | 45.81 | 29.74 | 154.02 |
| Carvacrol 1: benzene kresoxim-methyl30 | 2.66 | 61.65 | 30.31 | 203.42 |
| Carvacrol 1: kresoxim-methyl 20 | 1.13 | 145.13 | 31.42 | 461.98 |
| Carvacrol 1: benzene kresoxim-methyl 15 | 2.08 | 78.85 | 32.49 | 242.70 |
| Carvacrol 1: kresoxim-methyl 10 | 3.57 | 45.94 | 34.53 | 133.03 |
| Carvacrol 1: benzene kresoxim-methyl 5 | 1.41 | 116.31 | 39.99 | 290.86 |
| Carvacrol 1: kresoxim-methyl benzene 1 | 1.20 | 136.67 | 63.99 | 213.56 |
| Carvacrol 3: kresoxim-methyl benzene 1 | 0.72 | 227.78 | 82.00 | 277.79 |
| Carvacrol 5: kresoxim-methyl benzene 1 | 0.55 | 298.18 | 88.00 | 338.85 |
| Carvacrol 7: kresoxim-methyl benzene 1 | 0.69 | 237.68 | 91.00 | 261.19 |
| Carvacrol 9: kresoxim-methyl benzene 1 | 0.84 | 195.24 | 92.80 | 210.39 |
| Carvacrol 10: kresoxim-methyl benzene 1 | 0.93 | 176.34 | 93.45 | 188.70 |
| Carvacrol 20: kresoxim-methyl benzene 1 | 1.08 | 151.85 | 96.57 | 157.24 |
As can be seen from Table 1, the mass ratio of carvacrol to kresoxim-methyl is 1-20: the cotoxicity coefficients within the range of 40-1 are all larger than 120, namely the biological activity of the carvacrol and the kresoxim-methyl after being compounded to the disease of the millet white appears as a synergistic effect.
TABLE 2 indoor bioactivity assay of carvacrol and benzovindiflupyr combination on foxtail millet fungus
As can be seen from Table 2, the mass ratio of carvacrol to benzovindiflupyr is 1-60: the cotoxicity coefficients within the range of 40-1 are all larger than 120, namely the biological activity of the carvacrol and the benzovindiflupyr after being compounded shows synergistic effect on the white disease of the millet.
TABLE 3 determination of the indoor biological Activity of Cochinchinensis Scutellaria Evonoides and Biacetylenicamide
As can be seen from Table 3, the mass ratio of carvacrol to mandipropamid is 1-20: the cotoxicity coefficients within the range of 20-1 are all larger than 120, namely the biological activity of the compound of the carvacrol and the mandipropamid to the disease of the millet white appears as a synergistic effect.
In conclusion, when the carvacrol, the kresoxim-methyl benzoate, the benzovindiflupyr or the mandipropamid are compounded in a certain proportion, the biological activity of the compound preparation on the disease of the millet white is shown as a synergistic effect, the control effect of the disease of the millet white can be improved, and the application dosage of the pesticide can be reduced.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (4)
1. The bactericide composition for preventing and treating the disease of the millet white is characterized in that the active ingredients of the bactericide composition are prepared by binary compounding of carvacrol, kresoxim-methyl, benzovindiflupyr or mandipropamid.
2. The bactericide composition for preventing and treating the onset of the foxtail millet according to claim 1, wherein the mass ratio of carvacrol to kresoxim-methyl is 1-20: 40-1.
3. The bactericide composition for preventing and treating the onset of foxtail millet according to claim 1, wherein the mass ratio of carvacrol to benzovindiflupyr is 1-60: 40-1.
4. The bactericide composition for preventing and treating the onset of the foxtail millet according to claim 1, wherein the mass ratio of carvacrol to mandipropamid is 1-20: 20-1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011398402.3A CN112438259A (en) | 2020-12-04 | 2020-12-04 | Bactericide composition for preventing and treating white disease of foxtail millet |
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| CN202011398402.3A CN112438259A (en) | 2020-12-04 | 2020-12-04 | Bactericide composition for preventing and treating white disease of foxtail millet |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114208830A (en) * | 2021-12-27 | 2022-03-22 | 山东贵合生物科技有限公司 | Bactericide containing metalaxyl and kresoxim-methyl and preparation method thereof |
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| CN107006473A (en) * | 2017-04-18 | 2017-08-04 | 邓付圆 | A kind of bactericidal composition containing Boscalid and carvacrol |
| CN107047563A (en) * | 2017-04-20 | 2017-08-18 | 广西南宁益土生物科技有限责任公司 | A kind of bactericidal composition for preventing and treating Zizyphus maurtiana powdery mildew |
| CN107996575A (en) * | 2017-11-30 | 2018-05-08 | 惠州市支持网知识产权运营有限公司 | A kind of complex composition of fluorine-containing ether bacterium acid amides and carvacrol |
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- 2020-12-04 CN CN202011398402.3A patent/CN112438259A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107006473A (en) * | 2017-04-18 | 2017-08-04 | 邓付圆 | A kind of bactericidal composition containing Boscalid and carvacrol |
| CN107047563A (en) * | 2017-04-20 | 2017-08-18 | 广西南宁益土生物科技有限责任公司 | A kind of bactericidal composition for preventing and treating Zizyphus maurtiana powdery mildew |
| CN107996575A (en) * | 2017-11-30 | 2018-05-08 | 惠州市支持网知识产权运营有限公司 | A kind of complex composition of fluorine-containing ether bacterium acid amides and carvacrol |
Non-Patent Citations (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114208830A (en) * | 2021-12-27 | 2022-03-22 | 山东贵合生物科技有限公司 | Bactericide containing metalaxyl and kresoxim-methyl and preparation method thereof |
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