CN113994972B - Composition for treating gummosis of nectarines - Google Patents
Composition for treating gummosis of nectarines Download PDFInfo
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- CN113994972B CN113994972B CN202111360742.1A CN202111360742A CN113994972B CN 113994972 B CN113994972 B CN 113994972B CN 202111360742 A CN202111360742 A CN 202111360742A CN 113994972 B CN113994972 B CN 113994972B
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- gummosis
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- thiabendazole
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticide research and development, and particularly relates to a composition for treating gummosis of nectarines. The composition for treating the gummosis of the nectarine comprises the effective components of thiabendazole and 2, 4-dichlorophenoxyacetic acid in a compounding way, wherein the mass of the thiabendazole and the 2, 4-dichlorophenoxyacetic acid is 1-15:0.03-0.1; the thiabendazole consists of zinc thiazole and thiophanate-methyl. The composition for treating the gummosis of the nectarines has obvious synergistic effect on the gummosis of the nectarines, can improve the control effect on the gummosis of the nectarines, reduces the using dosage of pesticides and saves the control cost.
Description
Technical Field
The invention belongs to the technical field of pesticide research and development, and particularly relates to a composition for treating gummosis of nectarines.
Background
Peach gummosis disease is also called as skin tumor disease and occurs in each peach region in China, and pathogenic bacteria of peach gummosis disease in China comprise Botryosyphhaeria dothidea, leptosphaeria prunei Woronicin and Cucurbitaria sp. Peach gummosis is the main disease of peach trees, and occurs at branch parts, with the main stem being the most serious. Wherein the branches growing in 1 year appear with nodular protrusions, the nodular protrusions crack in the next year and overflow glue solution, and the glue solution is mostly colorless and semitransparent viscid and turns into dark brown hard blocks. The branches grow for years and generate blister-shaped swelling, and the diseased part is necrotic and brownish and gummy. When the peach gummosis occurs, the tree vigor is often weakened, the fruit quality is reduced, and plants die in severe cases.
Chemical pesticides are common means for preventing and treating peach gummosis, but pathogenic bacteria of peach trees are easy to generate drug resistance by long-term application of a single chemical agent. The compounding of chemical pesticides with different components is a common method for developing novel pesticides, and a compounding formula with a synergistic effect is screened, so that the prevention and treatment effect on plant diseases and insect pests can be improved, and the generation of drug resistance of the plant diseases and insect pests can be delayed, for example: application number CN201510180974.7, which discloses a bactericidal composition containing coumoxystrobin and xinjunan and application thereof; the application number CN201610598645.9 discloses a bactericidal composition containing octyl bacteria amine and propiconazole and an application thereof, wherein 2 active ingredients with different action mechanisms are compounded to improve the control effect on pathogenic bacteria and delay the generation of drug resistance of the pathogenic bacteria.
The inventor discovers through a large amount of experimental researches that when the 2, 4-dichlorophenoxyacetic acid, the zinc thiazole and the thiophanate-methyl are compounded according to a certain mass ratio, the compound pesticide has a good prevention and treatment effect on peach gummosis, and no relevant report is found at present.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art that is already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a composition for treating nectarine gummosis, which has the advantages that the effective components show synergistic action within a certain mass ratio range, the prevention and treatment effect on the gummosis of peach trees can be improved, and meanwhile, the generation of drug resistance of pathogenic bacteria can be delayed.
In order to realize the purpose, the invention provides the following technical scheme:
the invention aims to provide a composition for treating bleeding disease of nectarines, which comprises the effective components of thiabendazole and 2, 4-dichlorophenoxyacetic acid in a compounding manner, wherein the mass of the thiabendazole and the 2, 4-dichlorophenoxyacetic acid is (1-15): 0.03-0.1; the thiabendazole consists of zinc thiazole and thiophanate-methyl.
Preferably, the mass ratio of the zinc thiazole to the thiophanate-methyl is 1-5:10-1.
Preferably, the mass ratio of the zinc thiazole to the thiophanate-methyl is 3:1.
the second objective of the present invention is to provide a pesticide preparation, which consists of the pesticide composition and agriculturally acceptable auxiliary agents, for those skilled in the art, the agriculturally acceptable auxiliary agents include wetting agent, dispersing agent, solvent, emulsifying agent, stabilizing agent, antifreezing agent, film-forming agent, etc., and other known substances which are beneficial to the effective ingredients to exert the pesticide effect stably in the preparation, and the specific ingredients and the dosage of the ingredients can be determined by experiments according to different dosage forms.
Preferably, the mass of the pesticide composition accounts for 1.5-80% of the total mass of the pesticide preparation.
Preferably, the pesticide preparation is in the form of wettable powder, suspending agent, water dispersible granule or emulsion in water.
Compared with the prior art, the invention has the following beneficial effects:
(1) The composition for treating the gummosis of the nectarines consists of three active ingredients with different action mechanisms, can effectively slow down the drug resistance of pathogenic bacteria of the gummosis of the nectarines, and reduces the drug resistance risk brought by singly using a single active ingredient.
(2) When the active ingredients in the composition for treating the gummosis of the nectarine are compounded, antagonism is avoided among the active ingredients, the synergistic effect is obvious, the control effect on the gummosis of the nectarine can be improved, the using dosage of pesticide is reduced, and the cost for controlling the gummosis of the nectarine is reduced.
Detailed Description
In the following, the technical solutions of the present invention will be described clearly and completely, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1: indoor bioactivity test of zinc thiazole and thiophanate methyl
Pathogenic bacteria to be tested: collecting the gummosis scab of the nectarine, and adopting a tissue isolation method to carry out isolated culture and purification on the scab, wherein the pathogenic bacteria of the nectarine is Botryosypherio dothidea after identification.
Reagent to be tested: 95 percent of zinc thiazole raw drug and 95 percent of thiophanate methyl raw drug are all sold in the market;
the test method comprises the following steps: hypha growth rate method
1. Dissolving the raw materials with solvent, diluting with 0.1% Tween-80, respectively preparing single mother liquor, setting multiple groups of ratios, and setting 7 gradient mass concentrations for each single agent and each group of ratios according to equal ratio method.
2. Under the aseptic condition, quantitatively adding a pre-melted PDA culture medium into a sterile conical flask, quantitatively sucking liquid medicines from low concentration to high concentration in sequence, adding into the conical flasks respectively, shaking thoroughly, pouring into 4 culture dishes with the diameter of 9cm in equal amount to prepare a medicine-containing flat plate, setting a treatment only containing 0.1% Tween-80 as a blank control, and repeating each treatment for 3 times.
3. Cutting off a bacterial cake from the edge of a bacterial colony by using a sterilization puncher with the diameter of 5mm under an aseptic condition; inoculating the fungus cake in the center of the medicated plate with the hypha facing upwards, covering with a dish, and culturing in an incubator.
4. When the diameter of the blank control colony is more than 2/3 of the diameter of the culture dish, measuring the diameter of the colony by using a caliper, vertically measuring the diameter of each colony by using a cross method once, taking the average value, calculating the hypha growth inhibition rate of each treatment, performing regression analysis on the logarithm value of the medicament concentration and the corresponding hypha growth inhibition rate value by using DPS software, calculating the EC50 of each treatment medicament, and calculating the cotoxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.
D＝D 1 -D 2
In the above formula: d- -colony growth diameter; d 1 -colony diameter; d 2 - -the diameter of the cake.
In the above formula: i- -hyphal inhibition
The ratio; d0- -blank control colony growth diameter; d1- -agent treated colony growth diameter.
In the above formula: ATI- -actual virulence index of the combination; s- -EC of Standard drug 50 The unit is mg/L; m- -EC of test agent 50 The unit is mg/L.
TTI＝TI A ×P A +TI B ×P B
In the above formula: TTI- -theoretical toxicological index of the compound; TI A -the virulence index of the agent a; p A -the percentage of agent a in the mixture in units of percentage (%); TI B -virulence index of the B agent; p is B In admixture with-B agentsPercent in units of percent (%).
In the above formula: CTC-co-toxicity coefficient;
ATI- -measured virulence index of the mixture; TTI- -mixture theory virulence index.
The synergistic effect of the mixed medicament is evaluated according to the co-toxicity coefficient (CTC), namely the CTC is equal to or less than 80 and is antagonistic, the CTC is more than 80 and is less than 120 and is additive, and the CTC is equal to or more than 120 and is synergistic. The test results are shown in table 1 below.
TABLE 1 indoor determination of bioactivity of zinc thiazole and thiophanate-methyl on gummosis pathogens
As can be seen from Table 1, the zinc thiazoles and thiophanate-methyl are present in the ratios of 1-5: when the composition is compounded within the mass ratio range of 10-1, the co-toxicity coefficient to the pathogenic bacteria of the gummosis of the nectarine is more than 120, the synergistic effect is shown, and particularly, the composition has the following mass ratio of 3: when 1, the co-toxicity coefficient reaches 569.65, and the synergistic effect is particularly obvious.
Example 2: indoor bioactivity test of thiabendazole and 2, 4-dichlorophenoxyacetic acid compound
Pathogenic bacteria to be tested: the same as example 1;
reagent to be tested: 95 percent of thiazole zinc original drug, 95 percent of thiophanate-methyl original drug and 97.5 percent of 2, 4-dichlorophenoxyacetic acid original drug are all sold in the market;
the test method comprises the following steps: referring to example 1, the test results are shown in Table 2 below.
TABLE 2 indoor bioactivity assay of the complex of thiabendazole and 2, 4-dichlorophenoxyacetic acid against the causative bacteria of gummosis oleaginea
| Name and proportion of the medicament | EC50(mg/L) | ATI | TTI | CTC |
| Thiabendazole (zinc thiazole: thiophanate-methyl 3 | 2.48 | 100.00 | -- | -- |
| 2, 4-Dichlorophenoxyacetic acid | 243.59 | 1.02 | -- | -- |
| Thia-ling: 2, 4-Dichlorophenoxyacetic acid (1 | 2.16 | 114.81 | 91.00 | 126.17 |
| Thialing: 2, 4-Dichlorophenoxyacetic acid (1 | 2.01 | 123.38 | 93.52 | 131.93 |
| Thialing: 2, 4-Dichlorophenoxyacetic acid (1 | 1.85 | 134.05 | 95.29 | 140.69 |
| Thia-ling: 2, 4-Dichlorophenoxyacetic acid (1 | 0.82 | 302.44 | 97.12 | 311.42 |
| Thia-ling: 2, 4-Dichlorophenoxyacetic acid (3 | 0.53 | 467.92 | 99.02 | 472.56 |
| Thia-ling: 2, 4-Dichlorophenoxyacetic acid (5 | 0.76 | 326.32 | 99.41 | 328.25 |
| Thia-ling: 2, 4-Dichlorophenoxyacetic acid (10 | 1.27 | 195.28 | 99.70 | 195.86 |
| Thialing: 2, 4-Dichlorophenoxyacetic acid (15 | 0.76 | 326.32 | 99.80 | 326.96 |
As can be seen from Table 1, thiabendazole and 2, 4-dichlorophenoxyacetic acid are present in the ratio of 1-15: when the composition is compounded in the mass ratio range of 0.03-0.1, the co-toxicity coefficient to pathogenic bacteria of the nectarine gummosis is more than 120, and the synergistic effect is shown.
In conclusion, when the three active ingredients in the composition for treating the nectarine gummosis are compounded, the composition has obvious synergistic effect, can improve the control effect on the nectarine gummosis and delay the generation of drug resistance of pathogenic bacteria.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (1)
1. A composition for preventing and treating gummosis of nectarineBotryosphaeria dothideaThe application of the caused gummosis of nectarines is characterized in that the effective components of the composition are compounded by thiabendazole and 2, 4-dichlorophenoxyacetic acid, and the mass ratio of the thiabendazole to the 2, 4-dichlorophenoxyacetic acid is 1-15:0.03-0.1; the thiabendazole consists of zinc thiazole and thiophanate-methyl; the mass ratio of the zinc thiazole to the thiophanate-methyl in the thiabendazole is 3:1.
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