CN117099780A - Bactericidal composition containing aloe-emodin - Google Patents

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing aloe-emodin. A bactericidal composition containing aloe-emodin contains magnolol or carvacrol and aloe-emodin Huang Sufu as effective components. According to the invention, magnolol or carvacrol and aloe-emodin are compounded according to a certain mass ratio, so that a synergistic effect is shown on pathogenic bacteria of various plant fungal diseases, and the control effect on plant diseases can be improved.

Description

Bactericidal composition containing aloe-emodin

Technical field

The invention belongs to the technical field of pesticides, and specifically relates to a bactericidal composition containing aloe-emodin.

Background technique

In the process of crop cultivation, pests and diseases have always been an important issue affecting their yield and quality. Especially in recent years, with the continuous adjustment of the agricultural industry structure and the effective application of advanced technology, farmers have been stimulated to carry out agricultural planting. However, in the process of crop cultivation, due to unreasonable cultivation models, various diseases and pests have appeared. High epidemic situation. Effective prevention and control of various agricultural pests, including plant diseases, agricultural pests, and farmland weeds, is a prominent issue currently faced in agricultural production.

In the process of crop disease prevention and control, chemical control is currently the main method. However, due to the unscientific use of chemical pesticides during the prevention and control process, pathogenic bacteria have developed resistance to them, polluted the environment, and destroyed ecological security. A series of outstanding problems. With the introduction of the concept of green prevention and control, screening of environmentally friendly and efficient botanical fungicides has become a powerful means to prevent and treat crop diseases, and it also has the advantages of safety and environmental protection.

Botanical fungicides refer to active ingredients extracted from plants, which can effectively inhibit the effects of pathogens. They are highly efficient, low-toxic, and easily degradable in nature, and are favored by plant protection workers. At present, there are botanical fungicide products such as kasugamycin, eugenol, and ethosalin on the market, and they have been promoted and applied in the prevention and control of various crop diseases and have achieved remarkable results. Zhang Jing et al. found in field experiments that acetoallin has a control effect of more than 80% on soybean bacterial spot disease. Sun Xuemei found that when different chemicals were used to treat strawberry angular spot disease, the control effects of ningnanmycin, ethosin, zhongshengmycin and kasugamycin were all as high as 80%. At the same time, Zheng Anke et al. conducted a field test on the control effectiveness of 9 botanical fungicides against sunflower rust. The results showed that the average control effect of eugenol, garlic oil and osthole on sunflower rust was as high as over 80%, showing that Excellent field control effect.

Aloe belongs to the genus Aloe in the Liliaceae family, a fleshy evergreen herbaceous dicotyledonous plant. Common aloe products used in medicinal applications include Aloe vera, Aloe vera, Aloe vera, Aloe vera, Aloe arborescens, etc. Aloe vera is rich in anthraquinones and flavonoids and has anti-tumor, anti-inflammatory, immune-modulating and other effects. Aloe-emodin, also known as aloe-emodin, has multiple phenolic hydroxyl groups. Medical research has confirmed that it has anti-tumor, antibacterial, anti-diarrheal, free radical scavenging, lipid-lowering, anti-dandruff and Glossy hair and other effects. At present, there are few reports on the role of aloe-emodin in the prevention and control of crop diseases.

In order to further clarify the antibacterial effect of aloe-emodin on common plant diseases, the inventor's research group systematically carried out studies on the effects of aloe-emodin on wheat scab, wheat stem rot, peanut root rot, peanut white silkworm and corn. Analysis and research on the antibacterial activity of major crop diseases such as scab, the results show that a single dose of aloe-emodin has good antibacterial activity against the above-mentioned plant pathogenic fungi.

In order to further explore whether the mixture of aloe-emodin and commonly used botanical fungicides has a synergistic effect on major crop diseases such as wheat scab, wheat stem rot, peanut root rot, peanut white leaf blight and corn scab, The present invention has carried out a mixing test of aloe-emodin and commonly used botanical fungicides. The results show that the mixture of aloe-emodin and commonly used botanical fungicides has a significant synergistic effect on common major crop diseases such as wheat stem rot.

The information disclosed in this Background section is merely intended to enhance an understanding of the general background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art that is already known to a person of ordinary skill in the art.

Contents of the invention

The object of the present invention is to provide a bactericidal composition containing aloe-emodin, which can effectively inhibit the growth of pathogenic bacteria such as wheat stem rot, wheat scab, peanut root rot, peanut white spot, and corn scab. , effectively prevent and control a variety of plant fungal diseases, which is of great significance to the high quality and high yield of crops such as wheat, peanuts and corn.

In order to achieve the above objects, the present invention provides the following technical solutions:

A bactericidal composition containing aloe-emodin, the active ingredient of which is compounded of honokiol or carvacrol and aloe-emodin.

Preferably, the mass ratio of magnolol and aloe-emodin is 1-9:9-1.

Preferably, the mass ratio of carvacrol to aloe-emodin is 1-9:9-1.

The invention also provides the use of the aloe-emodin-containing bactericidal composition in preventing and treating fungal diseases of crops.

Preferably, the fungal diseases of crops include wheat stem rot, wheat scab, peanut root rot, peanut white leaf blight and corn scab.

Compared with the prior art, the present invention has the following beneficial effects:

In the present invention, honokiol or carvacrol and aloe-emodin are compounded at a certain mass ratio, which exhibits a synergistic effect on a variety of plant fungal disease pathogens, can improve the control effect on plant diseases, and provides a wheat stem base The effective prevention and control of rot, wheat scab, peanut root rot, peanut white blight and corn scab provides data support and is of great significance to the high quality and high yield of wheat, peanuts, corn and other crops.

Detailed ways

The following is a clear and complete description of the technical solution of the patent of the present invention.

Example

1. Test strain

Table 1 Test strains

2. Test reagents

98% magnolol technical material (Shanghai Aladdin Biochemical Technology Co., Ltd.), 92.5% carvacrol technical material (Shanghai Yuanye Biotechnology Co., Ltd.), 95% aloe-emodin technical material (Shanghai Yuanye Biotechnology Co., Ltd. ).

After the test reagent is dissolved, dilute it with 0.1% Tween-80 aqueous solution into a single-dose mother liquor, and set multiple groups of proportions. Each single-dose mother liquor and proportioned mixture are set in 5 mass concentration gradients according to the equal ratio method.

3.Test method

Add 9 mL of the pre-melted PDA culture medium into a sterile Erlenmeyer flask, quantitatively pipette 1 mL of the solution from low concentration to high concentration, add them to the above Erlenmeyer flasks, shake well, and pour into a culture medium with a diameter of 9 cm. In the dish, drug-containing plates of corresponding concentrations were made, and treatments without drugs were set as blank controls. Each mass concentration of drug solution was used to process 10 plates. Use a 5 mm diameter hole punch to cut out bacterial cakes from the edge of the colony of the test strain, inoculate them into the center of the drug-containing plate and the blank control plate, cover the plates and place them in a 25°C incubator for cultivation. When the diameter of the blank control colony grows to about 2/3 of the diameter of the petri dish, use the cross method to measure the diameter of the colony and calculate the inhibition rate of mycelium growth by different treatments.

4.Data analysis

DPS software was used for statistical analysis of data to obtain the virulence regression equation and the EC ₅₀ value of the agent's virulence against the target bacteria, and the cotoxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

5. Measurement results

The synergistic effect of the agent is evaluated according to the calculated co-toxicity coefficient (CTC). CTC ≤ 80 is an antagonistic effect, 80 < CTC < 120 is an additive effect, and CTC ≥ 120 is a synergistic effect. The results are shown in Table 2-9.

Table 2 Indoor biological activity determination of the compound of honokiol and aloe-emodin in Sclerotinia sclerotiorum

It can be seen from Table 2 that when honokiol is compounded with aloe-emodin, within the mass ratio range of 1-9:9-1, the co-toxicity coefficients of all tested compound combinations are greater than 120, and the performance produce a synergistic effect.

Table 3 Indoor biological activity determination of carvacrol and aloe-emodin combined with Sclerotinia sclerotiorum

Drug name and proportion EC ₅₀ (mg/L) ATI TTI CTC carvacrol 48.2768 100.0000 -- -- aloe emodin 202.7300 23.8133 -- -- Carvacrol 9: Aloe-Emodin 1 32.1847 149.9992 92.3813 162.3696 Carvacrol 8: Aloe-Emodin 2 29.7089 162.4995 84.7627 191.7111 Carvacrol 7: Aloe-Emodin 3 11.0347 437.4999 77.1440 567.1210 Carvacrol 6: Aloe-Emodin 4 14.3043 337.4985 69.5253 485.4324 Carvacrol 5: Aloe Emodin 5 8.5973 561.5344 61.9067 907.0661 Carvacrol 4: Aloe-Emodin 6 5.1495 937.5046 54.2880 1726.9092 Carvacrol 3: Aloe-Emodin 7 4.6532 1037.4968 46.6693 2223.0799 Carvacrol 2: Aloe Emodin 8 7.1980 670.6974 39.0507 1717.5052 Carvacrol 1: Aloe Emodin 9 3.4178 1412.5110 31.4320 4493.8610

It can be seen from Table 3 that when carvacrol is compounded with aloe-emodin, within the mass ratio range of 1-9:9-1, the co-toxicity coefficients of all tested compound combinations are greater than 120, and the performance There is a synergistic effect; especially when the mass ratio is 1:9, the co-toxicity coefficient of the compound combination reaches 4493.8610, and the synergistic effect is the most significant.

Table 4 Indoor biological activity determination of honokiol and aloe-emodin compound against Fusarium graminearum

It can be seen from Table 4 that when honokiol and aloe-emodin are compounded, when the mass ratio is 9:1, the co-toxicity coefficient of the compound combination against Fusarium graminearum is less than 80, showing an antagonistic effect; the other compound combinations The co-toxicity coefficients against Fusarium graminearum are all greater than 120, showing a synergistic effect.

Table 5 Indoor biological activity determination of carvacrol and aloe-emodin compound against Fusarium graminearum

Drug name and proportion EC ₅₀ (mg/L) ATI TTI CTC carvacrol 24.9855 100.0000 -- -- aloe emodin 285.5007 8.7515 -- -- Carvacrol 9: Aloe-Emodin 1 12.4928 199.9992 90.8751 220.0813 Carvacrol 8: Aloe-Emodin 2 18.7391 133.3335 81.7503 163.0985 Carvacrol 7: Aloe-Emodin 3 8.1034 308.3335 72.6254 424.5531 Carvacrol 6: Aloe-Emodin 4 14.2774 175.0004 63.5006 275.5886 Carvacrol 5: Aloe Emodin 5 6.1189 408.3332 54.3757 750.9475 Carvacrol 4: Aloe-Emodin 6 13.5318 184.6428 45.2509 408.0425 Carvacrol 3: Aloe-Emodin 7 19.4161 128.6844 36.1260 356.2098 Carvacrol 2: Aloe Emodin 8 16.6570 150.0000 27.0012 555.5314 Carvacrol 1: Aloe Emodin 9 20.4514 122.1701 17.8763 683.4187

It can be seen from Table 5 that when carvacrol is compounded with aloe-emodin, when the mass ratio is 1-9:9-1, the co-toxicity coefficient of the compound combination against Fusarium graminearum is greater than 120, showing a synergistic effect.

Table 6 Indoor biological activity determination of the compound of honokiol and aloe-emodin against Fusarium stratum corneum

Drug name and proportion EC ₅₀ (mg/L) ATI TTI CTC Honokiol 6.2200 100.0000 -- -- aloe emodin 127.1404 4.8922 -- -- Honokiol 9: Aloe-emodin 1 3.7728 164.8643 90.4892 182.1922 Honokiol 8: Aloe-Emodin 2 4.6530 133.6772 80.9784 165.0775 Honokiol 7: Aloe-emodin 3 5.3422 116.4314 71.4677 162.9148 Honokiol 6: Aloe-emodin 4 6.7535 92.1004 61.9569 148.6524 Honokiol 5: Aloe-Emodin 5 9.6131 64.7034 52.4461 123.3711 Honokiol 4: Aloe-emodin 6 5.9010 105.4059 42.9353 245.4991 Honokiol 3: Aloe-emodin 7 6.3928 97.2970 33.4246 291.0942 Honokiol 2: Aloe-emodin 8 6.2200 100.0000 23.9138 418.1689 Honokiol 1: Aloe emodin 9 5.0563 123.0149 14.4030 854.0915

It can be seen from Table 6 that when honokiol is compounded with aloe-emodin, when the mass ratio is within the mass ratio range of 1-9:9-1, the co-toxicity coefficients of the compound combination against Fusarium aeruginosa are all greater than 120, showing synergistic effect.

Table 7 Indoor biological activity determination of carvacrol and aloe-emodin combined with Fusarium stratum corneum

It can be seen from Table 7 that when carvacrol is compounded with aloe-emodin, within the mass ratio range of 1-9:9-1, the co-toxicity coefficients of all tested compound combinations against Fusarium aeruginosa are greater than 120. The performance produce a synergistic effect.

Table 8 Indoor biological activity determination of honokiol and aloe-emodin compound against Fusarium graminearum

It can be seen from Table 8 that when honokiol is compounded with aloe-emodin, in the mass range of 1-9:9-1, the co-toxicity coefficients of all compound combinations against Fusarium pseudograminearum are greater than 120, showing synergy. effect.

Table 9 Indoor biological activity determination of carvacrol and aloe-emodin compound against Fusarium graminearum

It can be seen from Table 9 that when carvacrol is compounded with aloe-emodin, in the mass range of 3-9:7-1, the co-toxicity coefficients of all tested compound combinations against Fusarium graminearum are greater than 120. The performance produce a synergistic effect.

In summary, the present invention combines magnolol or carvacrol with aloe-emodin at a certain mass ratio, which shows a synergistic effect on various plant fungal disease pathogens and can improve the control effect of plant diseases. , which provides data support for the effective prevention and control of wheat stem rot, wheat scab, peanut root rot, peanut white blight and corn scab, and is important for the high quality and high yield of wheat, peanuts, corn and other crops. meaning.

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and illustration. These descriptions are not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teachings. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical applications, thereby enabling others skilled in the art to make and utilize various exemplary embodiments of the invention and various different applications. Choice and change. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims (5)

1. A bactericidal composition containing aloe-emodin, characterized in that its active ingredient is compounded of honokiol or carvacrol and aloe-emodin. 2. The bactericidal composition containing aloe-emodin according to claim 1, characterized in that the mass ratio of honokiol and aloe-emodin is 1-9:9-1. 3. The bactericidal composition containing aloe-emodin according to claim 1, characterized in that the mass ratio of carvacrol to aloe-emodin is 1-9:9-1. 4. Use of the aloe-emodin-containing bactericidal composition according to any one of claims 1 to 3 in preventing and treating fungal diseases of crops. 5. The use according to claim 4, characterized in that the fungal diseases of crops include wheat stem rot, wheat scab, peanut root rot, peanut white silkworm and corn stem rot.