CN117099780A - Bactericidal composition containing aloe-emodin - Google Patents
Bactericidal composition containing aloe-emodin Download PDFInfo
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- CN117099780A CN117099780A CN202311134911.9A CN202311134911A CN117099780A CN 117099780 A CN117099780 A CN 117099780A CN 202311134911 A CN202311134911 A CN 202311134911A CN 117099780 A CN117099780 A CN 117099780A
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- China
- Prior art keywords
- aloe
- emodin
- carvacrol
- magnolol
- bactericidal composition
- Prior art date
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- YDQWDHRMZQUTBA-UHFFFAOYSA-N Aloe emodin Chemical compound C1=CC=C2C(=O)C3=CC(CO)=CC(O)=C3C(=O)C2=C1O YDQWDHRMZQUTBA-UHFFFAOYSA-N 0.000 title claims abstract description 119
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 22
- 239000000203 mixture Substances 0.000 title claims abstract description 14
- VVOAZFWZEDHOOU-UHFFFAOYSA-N magnolol Chemical compound OC1=CC=C(CC=C)C=C1C1=CC(CC=C)=CC=C1O VVOAZFWZEDHOOU-UHFFFAOYSA-N 0.000 claims abstract description 55
- 235000007746 carvacrol Nutrition 0.000 claims abstract description 39
- HHTWOMMSBMNRKP-UHFFFAOYSA-N carvacrol Natural products CC(=C)C1=CC=C(C)C(O)=C1 HHTWOMMSBMNRKP-UHFFFAOYSA-N 0.000 claims abstract description 39
- WYXXLXHHWYNKJF-UHFFFAOYSA-N isocarvacrol Natural products CC(C)C1=CC=C(O)C(C)=C1 WYXXLXHHWYNKJF-UHFFFAOYSA-N 0.000 claims abstract description 39
- RECUKUPTGUEGMW-UHFFFAOYSA-N carvacrol Chemical compound CC(C)C1=CC=C(C)C(O)=C1 RECUKUPTGUEGMW-UHFFFAOYSA-N 0.000 claims abstract description 37
- 208000031888 Mycoses Diseases 0.000 claims abstract description 8
- 241000209140 Triticum Species 0.000 claims description 18
- 235000021307 Triticum Nutrition 0.000 claims description 18
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 17
- 244000105624 Arachis hypogaea Species 0.000 claims description 17
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 17
- 235000018262 Arachis monticola Nutrition 0.000 claims description 17
- 235000020232 peanut Nutrition 0.000 claims description 17
- 206010039509 Scab Diseases 0.000 claims description 13
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- 239000004480 active ingredient Substances 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 14
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 12
- 230000002195 synergetic effect Effects 0.000 abstract description 12
- 244000052616 bacterial pathogen Species 0.000 abstract description 5
- 239000000575 pesticide Substances 0.000 abstract description 3
- 235000011399 aloe vera Nutrition 0.000 description 24
- 244000144927 Aloe barbadensis Species 0.000 description 21
- 235000002961 Aloe barbadensis Nutrition 0.000 description 21
- 241000196324 Embryophyta Species 0.000 description 16
- 239000003814 drug Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 10
- 150000001875 compounds Chemical class 0.000 description 9
- 230000004071 biological effect Effects 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 239000003899 bactericide agent Substances 0.000 description 6
- 230000002265 prevention Effects 0.000 description 6
- 241000223195 Fusarium graminearum Species 0.000 description 5
- 241001530056 Athelia rolfsii Species 0.000 description 4
- 241000223218 Fusarium Species 0.000 description 4
- 241001451172 Fusarium pseudograminearum Species 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 description 4
- OICGFSUWXCJLCO-UHFFFAOYSA-N 1-ethylsulfonylsulfanylethane Chemical compound CCSS(=O)(=O)CC OICGFSUWXCJLCO-UHFFFAOYSA-N 0.000 description 3
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 3
- 241001116389 Aloe Species 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 description 2
- 239000005770 Eugenol Substances 0.000 description 2
- 244000020551 Helianthus annuus Species 0.000 description 2
- 235000003222 Helianthus annuus Nutrition 0.000 description 2
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 229960002217 eugenol Drugs 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- PVTHJAPFENJVNC-MHRBZPPQSA-N kasugamycin Chemical compound N[C@H]1C[C@H](NC(=N)C(O)=O)[C@@H](C)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)[C@@H]1O PVTHJAPFENJVNC-MHRBZPPQSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- AMNAZJFEONUVTD-KEWDHRJRSA-N (2s,3s,4s,5r,6r)-6-(4-amino-2-oxopyrimidin-1-yl)-4,5-dihydroxy-3-[[(2s)-3-hydroxy-2-[[2-(methylamino)acetyl]amino]propanoyl]amino]oxane-2-carboxamide Chemical compound O1[C@H](C(N)=O)[C@@H](NC(=O)[C@H](CO)NC(=O)CNC)[C@H](O)[C@@H](O)[C@@H]1N1C(=O)N=C(N)C=C1 AMNAZJFEONUVTD-KEWDHRJRSA-N 0.000 description 1
- 240000007474 Aloe arborescens Species 0.000 description 1
- 235000004509 Aloe arborescens Nutrition 0.000 description 1
- HKIKAXXIWJHWLY-ZIIYPAMZSA-N Aloesin Chemical compound C=12OC(CC(=O)C)=CC(=O)C2=C(C)C=C(O)C=1[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HKIKAXXIWJHWLY-ZIIYPAMZSA-N 0.000 description 1
- HKIKAXXIWJHWLY-QEVGBQTESA-N Aloesin Natural products O=C(CC=1Oc2c([C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)c(O)cc(C)c2C(=O)C=1)C HKIKAXXIWJHWLY-QEVGBQTESA-N 0.000 description 1
- 241001290235 Ceratobasidium cereale Species 0.000 description 1
- 208000001840 Dandruff Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- FEACDOXQOYCHKU-UHFFFAOYSA-N Gougerotin Natural products CNCC(=O)NC1=NC(=O)N(C=C1)C2OC(C(O)C(NC(=O)C(N)CO)C2O)C(=O)N FEACDOXQOYCHKU-UHFFFAOYSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- HPUXDMUGCAWDFW-UHFFFAOYSA-N Osthole Natural products COc1ccc2CCC(=O)Oc2c1C=CC(=O)C HPUXDMUGCAWDFW-UHFFFAOYSA-N 0.000 description 1
- VMFXMTJCTSYHCF-HHQUSWFZSA-N [(2r,3r,4s,5r)-5-(hexylamino)-4-hydroxy-2-(hydroxymethyl)-6-[(7-hydroxy-4-oxo-1,3a,5,6,7,7a-hexahydroimidazo[4,5-c]pyridin-2-yl)amino]oxan-3-yl] carbamate Chemical compound CCCCCCN[C@@H]1[C@H](O)[C@@H](OC(N)=O)[C@@H](CO)OC1\N=C\1NC(C(=O)NCC2O)C2N/1 VMFXMTJCTSYHCF-HHQUSWFZSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 1
- 150000004056 anthraquinones Chemical class 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005282 brightening Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- -1 flavonoid compounds Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 244000000004 fungal plant pathogen Species 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 239000010647 garlic oil Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000004957 immunoregulator effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- MBRLOUHOWLUMFF-UHFFFAOYSA-N osthole Chemical compound C1=CC(=O)OC2=C(CC=C(C)C)C(OC)=CC=C21 MBRLOUHOWLUMFF-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- OLBCVFGFOZPWHH-UHFFFAOYSA-N propofol Chemical compound CC(C)C1=CC=CC(C(C)C)=C1O OLBCVFGFOZPWHH-UHFFFAOYSA-N 0.000 description 1
- 229960004134 propofol Drugs 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing aloe-emodin. A bactericidal composition containing aloe-emodin contains magnolol or carvacrol and aloe-emodin Huang Sufu as effective components. According to the invention, magnolol or carvacrol and aloe-emodin are compounded according to a certain mass ratio, so that a synergistic effect is shown on pathogenic bacteria of various plant fungal diseases, and the control effect on plant diseases can be improved.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing aloe-emodin.
Background
During crop planting, pest and disease damage has been an important issue affecting the yield and quality thereof. Especially in recent years, along with continuous adjustment of agricultural industry structures and effective application of advanced technologies, enthusiasm of farmers for developing agricultural planting is stimulated, but in the process of crop cultivation, various diseases and insect pests are in a situation of high popularity due to unreasonable cultivation modes. The prevention and control work of various agricultural pests including plant diseases, agricultural pests, farmland weeds and the like are effectively carried out, and the method is a prominent problem facing the current agricultural production.
In the process of preventing and controlling crop diseases, chemical prevention and control are mainly adopted at present, but due to unscientific use of chemical pesticides in the prevention and control process, a series of outstanding problems of drug resistance, environmental pollution, ecological safety damage and the like are caused by pathogenic bacteria. Along with the proposal of the green prevention and control concept, the screening of the environment-friendly efficient plant source bactericide becomes a powerful means for preventing and controlling crop diseases, and has the advantages of safety, environmental protection and the like.
The botanical fungicide is an effective component extracted from plants, has the effect of effectively inhibiting pathogens, has the characteristics of high efficiency, low toxicity, easy degradation in the nature and the like, and is favored by plant protection workers. Plant source bactericide products such as kasugamycin, eugenol, ethylicin and the like are already available in the market at present, and are popularized and applied in the process of preventing and controlling various crop diseases, and remarkable effects are achieved. Zhang Jing and the like find that the control effect of the ethylicin on the bacterial spot disease of soybean is up to more than 80 percent in field experiments. Sun Xuemei it is found that when different medicines are used for dipping roots to treat strawberry angular leaf spot, the prevention effect of Ningnan mycin, ethylicin, zhongshengmycin and kasugamycin is up to more than 80%. Meanwhile, zheng Anke and the like develop field control effect tests of 9 plant source bactericides on sunflower rust, and the results show that the average control effect of eugenol, garlic oil and osthole on sunflower rust is up to more than 80%, and excellent field control effect is shown.
Aloe belongs to genus Aloe of Liliaceae, and is prepared from pulp evergreen herb dicotyledon, such as Aloe Vera, and Aloe arborescens etc. The aloe is rich in anthraquinone and flavonoid compounds, and has anti-tumor, antiinflammatory and immunoregulatory effects. Aloe-emodin (Aloe-emodin) is also known as aloesin, has a plurality of phenolic hydroxyl groups, and has been shown in medical research to have the effects of resisting tumors, resisting bacteria, resisting purgation, scavenging free radicals in human bodies, reducing fat and weight, removing dandruff, and brightening hair. At present, the effect of aloe-emodin in the crop disease prevention and control process is freshly reported.
In order to further determine the antibacterial effect of aloe-emodin on common plant diseases, the inventor's subject group system develops the antibacterial activity analysis and research of aloe-emodin on important crop diseases including wheat scab, wheat stem rot, peanut root rot, peanut southern blight, corn scab and the like, and the result shows that the aloe-emodin single agent has better antibacterial activity on the plant pathogenic fungi.
In order to further explore whether the aloe-emodin and the common plant source bactericide are mixed to have synergism on major crop diseases such as wheat scab, wheat stem rot, peanut root rot, peanut southern blight and corn scab, the invention develops a mixing test of the aloe-emodin and the common plant source bactericide, and the result shows that the aloe-emodin and the common plant source bactericide are mixed to have remarkable synergism on major crop diseases such as wheat stem rot and the like.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a bactericidal composition containing aloe-emodin, which can effectively inhibit the growth of pathogenic bacteria such as wheat stem rot, wheat scab, peanut root rot, peanut southern blight, corn scab and the like, effectively prevent and control various plant fungal diseases, and has important significance for the high quality and high yield of crops such as wheat, peanut, corn and the like.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a bactericidal composition containing aloe-emodin contains magnolol or carvacrol and aloe-emodin Huang Sufu as effective components.
Preferably, the mass ratio of magnolol to aloe-emodin is 1-9:9-1.
Preferably, the mass ratio of the carvacrol to the aloe-emodin is 1-9:9-1.
The invention also provides the application of the bactericidal composition containing aloe-emodin in preventing and controlling fungal diseases of crops.
Preferably, the crop fungal diseases include wheat stem rot, wheat scab, peanut root rot, peanut southern blight and corn scab.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, magnolol or carvacrol and aloe-emodin are compounded according to a certain mass ratio, a synergistic effect is shown on pathogenic bacteria of various plant fungal diseases, the control effect on plant diseases can be improved, data support is provided for effective prevention and control of wheat stem rot, wheat scab, peanut root rot, peanut southern blight and corn scab, and the invention has important significance on high quality and high yield of crops such as wheat, peanut and corn.
Detailed Description
The following is a clear and complete description of the technical solutions of the present patent.
Examples
1. Test strain
TABLE 1 test strains
2. Test agent
98% magnolol (Shanghai Ala Biotechnology Co., ltd.), 92.5% carvacrol (Shanghai Yuan Yes Biotechnology Co., ltd.), 95% aloe vera Huang Suyuan (Shanghai Yuan Yes Biotechnology Co., ltd.).
After the test agent is dissolved, diluting the test agent into a single-agent mother solution by using 0.1% Tween-80 aqueous solution, setting a plurality of groups of proportions, and setting 5 mass concentration gradients of each single-agent mother solution and each proportion mixture according to an equal proportion method.
3. Test method
Adding 9mL of pre-melted PDA culture medium into a sterile conical flask, sequentially quantitatively sucking 1mL of liquid medicine from low concentration to high concentration, respectively adding into the conical flask, fully shaking uniformly, pouring into 1 culture dishes with the diameter of 9cm, preparing medicine-containing flat plates with corresponding concentrations, simultaneously setting treatment without medicines as blank control, and treating 10 flat plates with each liquid medicine with the mass concentration. Cutting bacterial cake at the colony edge of the tested bacterial strain by using a puncher with the diameter of 5mm, inoculating the bacterial cake to the centers of a medicine-containing flat plate and a blank control flat plate, covering a dish cover, and culturing in a constant temperature oven at 25 ℃. When the diameter of the blank control colony grows to about 2/3 of the diameter of the culture dish, measuring the colony diameter by a crisscross method, and calculating the inhibition rate of different treatments on hypha growth.
4. Data analysis
Data statistics analysis is carried out by adopting DPS software to obtain a virulence regression equation and virulence EC of the medicament on target bacteria 50 Values and co-toxicity coefficients (CTCs) were calculated according to the grand cloud Pei method.
5. Measurement results
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 2-9.
TABLE 2 determination of indoor biological Activity of Propofol and Aloe Vera Huang Sufu pair-up sclerotium rolfsii
As can be seen from Table 2, when magnolol and aloe vera are larger Huang Sufu, the ratio of magnolol to aloe vera is 1-9: and in the mass ratio range of 9-1, the co-toxicity coefficient of all the tested compound combination aligned sclerotium rolfsii is more than 120, and the synergistic effect is shown.
TABLE 3 determination of indoor biological Activity of Parslemol and Aloe Vera Huang Sufu pair Rhizoctonia cerealis
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Carvacrol | 48.2768 | 100.0000 | -- | -- |
| Aloe-emodin | 202.7300 | 23.8133 | -- | -- |
| Carvacrol 9: aloe-emodin 1 | 32.1847 | 149.9992 | 92.3813 | 162.3696 |
| Carvacrol 8: aloe-emodin 2 | 29.7089 | 162.4995 | 84.7627 | 191.7111 |
| Carvacrol 7: aloe-emodin 3 | 11.0347 | 437.4999 | 77.1440 | 567.1210 |
| Carvacrol 6: aloe-emodin 4 | 14.3043 | 337.4985 | 69.5253 | 485.4324 |
| Carvacrol 5: aloe-emodin 5 | 8.5973 | 561.5344 | 61.9067 | 907.0661 |
| Carvacrol 4: aloe-emodin 6 | 5.1495 | 937.5046 | 54.2880 | 1726.9092 |
| Carvacrol 3: aloe-emodin 7 | 4.6532 | 1037.4968 | 46.6693 | 2223.0799 |
| Carvacrol 2: aloe-emodin 8 | 7.1980 | 670.6974 | 39.0507 | 1717.5052 |
| Carvacrol 1: aloe-emodin 9 | 3.4178 | 1412.5110 | 31.4320 | 4493.8610 |
As can be seen from Table 3, when carvacrol is mixed with aloe vera as large as Huang Sufu, the ratio of carvacrol to aloe vera is 1-9: in the mass ratio range of 9-1, the co-toxicity coefficient of all the tested compound combination aligned sclerotium rolfsii is more than 120, and the synergistic effect is shown; especially when the mass ratio is 1:9, the co-toxicity coefficient of the compound combined aligned sclerotium rolfsii reaches 4493.8610, and the synergistic effect is most remarkable.
Table 4 indoor biological Activity assay of Fusarium graminearum paired with magnolol and aloe vera Huang Sufu
As can be seen from table 4, when magnolol and aloe vera are larger Huang Sufu, the mass ratio is 9:1, the co-toxicity coefficient of the compound combination to fusarium graminearum is smaller than 80, and antagonism is shown; the co-toxicity coefficient of the other compound combinations on fusarium graminearum is more than 120, and the synergistic effect is shown.
TABLE 5 determination of indoor biological Activity of carvacrol and aloe vera with Fusarium graminearum paired with aloe vera Huang Sufu
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Carvacrol | 24.9855 | 100.0000 | -- | -- |
| Aloe-emodin | 285.5007 | 8.7515 | -- | -- |
| Carvacrol 9: aloe-emodin 1 | 12.4928 | 199.9992 | 90.8751 | 220.0813 |
| Carvacrol 8: aloe-emodin 2 | 18.7391 | 133.3335 | 81.7503 | 163.0985 |
| Carvacrol 7: aloe-emodin 3 | 8.1034 | 308.3335 | 72.6254 | 424.5531 |
| Carvacrol 6: aloe-emodin 4 | 14.2774 | 175.0004 | 63.5006 | 275.5886 |
| Carvacrol 5: aloe-emodin 5 | 6.1189 | 408.3332 | 54.3757 | 750.9475 |
| Carvacrol 4: aloe-emodin 6 | 13.5318 | 184.6428 | 45.2509 | 408.0425 |
| Carvacrol 3: aloe-emodin 7 | 19.4161 | 128.6844 | 36.1260 | 356.2098 |
| Carvacrol 2: aloe-emodin 8 | 16.6570 | 150.0000 | 27.0012 | 555.5314 |
| Carvacrol 1: aloe-emodin 9 | 20.4514 | 122.1701 | 17.8763 | 683.4187 |
As can be seen from Table 5, when the mass ratio of carvacrol to aloe vera is Huang Sufu, it is 1-9:9-1, the co-toxicity coefficient of the compound combination to fusarium graminearum is greater than 120, and the synergistic effect is shown.
Table 6 indoor biological Activity assay of Fusarium on the Copair of magnolol and aloe vera (Huang Sufu)
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Magnolol | 6.2200 | 100.0000 | -- | -- |
| Aloe-emodin | 127.1404 | 4.8922 | -- | -- |
| Magnolol 9: aloe-emodin 1 | 3.7728 | 164.8643 | 90.4892 | 182.1922 |
| Magnolol 8: aloe-emodin 2 | 4.6530 | 133.6772 | 80.9784 | 165.0775 |
| Magnolol 7: aloe-emodin 3 | 5.3422 | 116.4314 | 71.4677 | 162.9148 |
| Magnolol 6: aloe-emodin 4 | 6.7535 | 92.1004 | 61.9569 | 148.6524 |
| Magnolol 5: aloe-emodin 5 | 9.6131 | 64.7034 | 52.4461 | 123.3711 |
| Magnolol 4: aloe-emodin 6 | 5.9010 | 105.4059 | 42.9353 | 245.4991 |
| Magnolol 3: aloe-emodin 7 | 6.3928 | 97.2970 | 33.4246 | 291.0942 |
| Magnolol 2: aloe-emodin 8 | 6.2200 | 100.0000 | 23.9138 | 418.1689 |
| Magnolol 1: aloe-emodin 9 | 5.0563 | 123.0149 | 14.4030 | 854.0915 |
As can be seen from Table 6, when the mass ratio of magnolol to aloe vera is Huang Sufu, it is 1-9: in the mass ratio range of 9-1, the co-toxicity coefficients of the fusarium on the compounded combination pair layer are all larger than 120, and the synergistic effect is shown.
TABLE 7 determination of indoor biological Activity of Fusarium species paired with carvacrol and aloe vera Da Huang Sufu
As can be seen from Table 7, when carvacrol is mixed with aloe vera as large as Huang Sufu, the ratio of carvacrol to aloe vera is 1-9: in the mass ratio range of 9-1, the co-toxicity coefficient of all tested compound combination pairs layer fusarium is more than 120, and the synergistic effect is shown.
Table 8 determination of indoor biological Activity of Fusarium pseudograminearum paired with magnolol and aloe vera Large Huang Sufu
As can be seen from Table 8, when magnolol and aloe vera are Huang Sufu, the ratio of magnolol to aloe vera is 1-9: in the mass range of 9-1, the co-toxicity coefficient of all the compound combinations to the fusarium pseudograminearum is more than 120, and the synergistic effect is shown.
TABLE 9 determination of indoor biological Activity of Fusarium pseudograminearum paired with carvacrol and aloe vera Da Huang Sufu
As can be seen from Table 9, when carvacrol is mixed with aloe vera as large as Huang Sufu, the ratio of carvacrol to aloe vera is 3-9: in the mass range of 7-1, the co-toxicity coefficient of all tested compound combinations on fusarium pseudograminearum is more than 120, and the synergistic effect is shown.
In conclusion, the magnolol or carvacrol and aloe-emodin are compounded according to a certain mass ratio, a synergistic effect is shown on pathogenic bacteria of various plant fungal diseases, the control effect on plant diseases can be improved, data support is provided for effective control of wheat stem rot, wheat scab, peanut root rot, peanut southern blight and corn scab, and the method has important significance on high quality and high yield of crops such as wheat, peanut and corn.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (5)
1. A bactericidal composition containing aloe-emodin is characterized in that the active ingredients of the bactericidal composition are prepared from magnolol or carvacrol and aloe-emodin Huang Sufu.
2. The aloe-emodin containing bactericidal composition according to claim 1, wherein the mass ratio of magnolol to aloe-emodin is 1-9:9-1.
3. The aloe-emodin containing bactericidal composition according to claim 1, wherein the mass ratio of carvacrol to aloe-emodin is 1-9:9-1.
4. Use of the bactericidal composition containing aloe-emodin according to any one of claims 1-3 for controlling fungal diseases of crops.
5. The use according to claim 4, wherein the crop fungal diseases include wheat stem basal rot, wheat scab, peanut root rot, peanut southern blight and corn stem basal rot.
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