CN114651825A - Macadimia nut stem canker inhibitor based on molecular disease resistance approach - Google Patents
Macadimia nut stem canker inhibitor based on molecular disease resistance approach Download PDFInfo
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- CN114651825A CN114651825A CN202210199758.7A CN202210199758A CN114651825A CN 114651825 A CN114651825 A CN 114651825A CN 202210199758 A CN202210199758 A CN 202210199758A CN 114651825 A CN114651825 A CN 114651825A
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- inhibitor
- stem canker
- macadimia nut
- nut stem
- triadimenol
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- 239000003112 inhibitor Substances 0.000 title claims abstract description 23
- 208000024556 Mendelian disease Diseases 0.000 title claims abstract description 6
- 238000013459 approach Methods 0.000 title claims abstract description 6
- 239000005846 Triadimenol Substances 0.000 claims abstract description 21
- BAZVSMNPJJMILC-UHFFFAOYSA-N triadimenol Chemical compound C1=NC=NN1C(C(O)C(C)(C)C)OC1=CC=C(Cl)C=C1 BAZVSMNPJJMILC-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000005776 Fenhexamid Substances 0.000 claims abstract description 10
- VDLGAVXLJYLFDH-UHFFFAOYSA-N fenhexamid Chemical compound C=1C=C(O)C(Cl)=C(Cl)C=1NC(=O)C1(C)CCCCC1 VDLGAVXLJYLFDH-UHFFFAOYSA-N 0.000 claims abstract description 10
- 201000010099 disease Diseases 0.000 claims description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 11
- 229940103185 mefenamate Drugs 0.000 claims 1
- HYYBABOKPJLUIN-UHFFFAOYSA-N mefenamic acid Chemical compound CC1=CC=CC(NC=2C(=CC=CC=2)C(O)=O)=C1C HYYBABOKPJLUIN-UHFFFAOYSA-N 0.000 claims 1
- 230000009471 action Effects 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 8
- 244000052616 bacterial pathogen Species 0.000 abstract description 7
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 239000000575 pesticide Substances 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 3
- 230000007246 mechanism Effects 0.000 abstract description 3
- 206010034133 Pathogen resistance Diseases 0.000 abstract description 2
- 230000003111 delayed effect Effects 0.000 abstract description 2
- 239000000447 pesticide residue Substances 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract description 2
- 230000009044 synergistic interaction Effects 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 description 9
- 239000003814 drug Substances 0.000 description 8
- 239000000203 mixture Substances 0.000 description 7
- 241000233614 Phytophthora Species 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 230000002195 synergetic effect Effects 0.000 description 6
- 208000025865 Ulcer Diseases 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- DSSYKIVIOFKYAU-XCBNKYQSSA-N (R)-camphor Chemical compound C1C[C@@]2(C)C(=O)C[C@@H]1C2(C)C DSSYKIVIOFKYAU-XCBNKYQSSA-N 0.000 description 4
- IAQLCKZJGNTRDO-UHFFFAOYSA-N 1-(4-{4-[5-(2,6-difluorophenyl)-4,5-dihydro-1,2-oxazol-3-yl]-1,3-thiazol-2-yl}piperidin-1-yl)-2-[5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl]ethanone Chemical compound CC1=CC(C(F)(F)F)=NN1CC(=O)N1CCC(C=2SC=C(N=2)C=2CC(ON=2)C=2C(=CC=CC=2F)F)CC1 IAQLCKZJGNTRDO-UHFFFAOYSA-N 0.000 description 4
- 239000005812 Oxathiapiprolin Substances 0.000 description 4
- 241000233618 Phytophthora cinnamomi Species 0.000 description 4
- 229940025250 camphora Drugs 0.000 description 4
- 239000010238 camphora Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- QPJVMBTYPHYUOC-UHFFFAOYSA-N methyl benzoate Chemical compound COC(=O)C1=CC=CC=C1 QPJVMBTYPHYUOC-UHFFFAOYSA-N 0.000 description 4
- 231100000397 ulcer Toxicity 0.000 description 4
- 230000001018 virulence Effects 0.000 description 4
- KQTIMOAWZBAJLH-UHFFFAOYSA-N 2-fluoro-1,3-thiazole Chemical compound FC1=NC=CS1 KQTIMOAWZBAJLH-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000005739 Bordeaux mixture Substances 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 241000208467 Macadamia Species 0.000 description 2
- 235000018330 Macadamia integrifolia Nutrition 0.000 description 2
- 240000000912 Macadamia tetraphylla Species 0.000 description 2
- 235000003800 Macadamia tetraphylla Nutrition 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 229940095102 methyl benzoate Drugs 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001338 necrotic effect Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- LSPHULWDVZXLIL-UHFFFAOYSA-N (+/-)-Camphoric acid Chemical compound CC1(C)C(C(O)=O)CCC1(C)C(O)=O LSPHULWDVZXLIL-UHFFFAOYSA-N 0.000 description 1
- DARPYRSDRJYGIF-PTNGSMBKSA-N (Z)-3-ethoxy-2-naphthalen-2-ylsulfonylprop-2-enenitrile Chemical compound C1=CC=CC2=CC(S(=O)(=O)C(\C#N)=C/OCC)=CC=C21 DARPYRSDRJYGIF-PTNGSMBKSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- BOMXNVKTSSSTNJ-UHFFFAOYSA-N C1CC=NO1.C1CCN(CC1)c1nccs1 Chemical compound C1CC=NO1.C1CCN(CC1)c1nccs1 BOMXNVKTSSSTNJ-UHFFFAOYSA-N 0.000 description 1
- 239000005752 Copper oxychloride Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000233654 Oomycetes Species 0.000 description 1
- 241000233629 Phytophthora parasitica Species 0.000 description 1
- 241000233639 Pythium Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- JHRWWRDRBPCWTF-OLQVQODUSA-N captafol Chemical compound C1C=CC[C@H]2C(=O)N(SC(Cl)(Cl)C(Cl)Cl)C(=O)[C@H]21 JHRWWRDRBPCWTF-OLQVQODUSA-N 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- HKMOPYJWSFRURD-UHFFFAOYSA-N chloro hypochlorite;copper Chemical compound [Cu].ClOCl HKMOPYJWSFRURD-UHFFFAOYSA-N 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- OPGCOAPTHCZZIW-UHFFFAOYSA-N diethyl 1-(2,4-dichlorophenyl)-5-methyl-4h-pyrazole-3,5-dicarboxylate Chemical group CCOC(=O)C1(C)CC(C(=O)OCC)=NN1C1=CC=C(Cl)C=C1Cl OPGCOAPTHCZZIW-UHFFFAOYSA-N 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- XIGAUIHYSDTJHW-UHFFFAOYSA-N mefenacet Chemical compound N=1C2=CC=CC=C2SC=1OCC(=O)N(C)C1=CC=CC=C1 XIGAUIHYSDTJHW-UHFFFAOYSA-N 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/22—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
- A01N37/24—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides containing at least one oxygen or sulfur atom being directly attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a macadimia nut stem canker inhibitor based on a molecular disease-resistant approach. The effective components of the macadimia nut stem canker inhibitor are compounded by fluorothiazole pyrithylone, triadimenol, metrafoxanyl or fenhexamid. When two active ingredients in the macadimia nut stem canker inhibitor are compounded, the inhibitor has no cross resistance, shows good synergistic interaction effect, can improve the control effect of the macadimia nut stem canker, reduce the application dosage of pesticide, and simultaneously can reduce pesticide residue. In addition, the molecular action mechanisms and action sites of the fluorothiazole pyrithylone and other effective components in the macadimia nut stem canker inhibitor are different, when the inhibitor is used, pathogenic bacteria are killed under the combined action of multiple sites, the generation of drug resistance of the pathogenic bacteria can be delayed, and the life cycle of the inhibitor can be prolonged.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a macadimia nut stem canker inhibitor based on a molecular disease-resistant approach.
Background
Macadamia nut is a nut plant originally produced in macadamia, and has high economic value, high nutritional value and high medicinal value. The macadimia nuts have more diseases and insect pests, more than 30 diseases and more than 300 diseases, and the more serious diseases include damping off, root rot, flower blight, anthracnose, ulcer and gray mold. The macadimia nut stem canker is caused by infection of the basal part, stem and main branch of the macadimia nut by Phytophthora camphora (Phytophthora cinnamami Rands), the stem or branch near the ground is infected firstly, the bark of the diseased part is browned and hardened, and the formed layer is necrotic. The disease is marked by marked division, and then the center of the lesion is sunken, and dark brown sticky jelly is exuded, the surface is severely shrunk, and ulcer spots are formed. The xylem under the bark turns brown, and the bark of the diseased part cracks in the later period; after the disease spots expand to surround the stem or the lateral branch for one week, the leaves of the diseased tree are faded, dull, poor in growth potential and short, partial leaf and fruit dropping phenomena occur, and seriously diseased branches die or the whole plant dies.
In the planting process, the commonly used chemical control means are as follows: scraping necrotic bark and xylem tissue from ulcer, coating wound with copper oxychloride slurry (25g/L) or equivalent Bordeaux mixture (1: 1: 25), wrapping, and spraying stem with 1% equivalent Bordeaux mixture or 80% wettable captafol. However, due to the long-term application of a single chemical agent, the macadimia nut stem canker has different degrees of drug resistance to the currently used agents, so that the prevention and treatment effect of the current agents on the macadimia nut stem canker is poor.
The fluorothiazole pyrithylone is a piperidyl thiazole isoxazoline bactericide and has both protection and treatment activities. It has bactericidal effect through inhibiting oxysterol conjugated protein, novel action site, and high effect on plant diseases caused by oomycetes pathogenic bacteria. The fluorothiazole pyrithylone has a novel action mechanism, so that the fluorothiazole pyrithylone can be used for a resistance treatment strategy, but the action site is single, so that the fluorothiazole pyrithylone has high-level resistance risk, and if the fluorothiazole pyrithylone is applied for a long time, diseases are easy to generate drug resistance, and the life cycle of the medicament is shortened.
Disclosure of Invention
The invention aims to provide a macadimia nut stem canker inhibitor based on a molecular disease-resistant approach, which aims to solve the problem that the macadimia nut stem canker has different degrees of drug resistance to currently used medicaments, so that the control effect of the current medicaments on the macadimia nut stem canker is poor.
In order to achieve the purpose, the invention provides the following technical scheme:
the effective components of the macadimia nut stem canker inhibitor are compounded by fluorothiazole pyrithylone, triadimenol, metrafoxanyl or fenhexamid.
Preferably, the mass ratio of the fluorothiazole pyrithylone to the triadimenol is 1-50: 40-1.
Preferably, the mass ratio of the fluorothiazole pyrithylone to the triadimenol is 1: 5.
preferably, the mass ratio of the oxathiapiprolin to the mefenpyr-diethyl is 1-8: 20-1.
Preferably, the mass ratio of the oxathiapiprolin to the fenhexamid is 1-5: 16-1.
Compared with the prior art, the invention has the following beneficial effects:
(1) when two active ingredients in the macadimia nut stem ulcer disease inhibitor are compounded, the inhibitor has no cross resistance, shows good synergistic interaction, can improve the control effect of the macadimia nut stem ulcer, reduces the application dosage of pesticide, and can reduce pesticide residues.
(2) The molecular action mechanisms and action sites of the fluorothiazole pyrithylone and other effective components in the macadimia nut stem canker inhibitor are different, and when the inhibitor is used, pathogenic bacteria are killed by the combined action of multiple sites, so that the generation of drug resistance of the pathogenic bacteria can be delayed, and the life cycle of the inhibitor can be prolonged.
Detailed Description
In the following, the technical solutions of the present invention will be described clearly and completely, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
Reagent to be tested: 95% of fluorothiazole pyrithylone (Kedithua agricultural science and technology, Inc.), 97% of triadimenol (Jiangsu Jianpai agricultural chemical Co., Ltd.), 95% of toluidinol (Shenyang chemical research institute, Inc.), and 98% of fenhexamid (Shaanxi Hengrun chemical industry, Inc.).
Pathogenic bacteria to be tested: the bacterial strain is collected from the occurrence part of the macadamia stem canker, is determined to be Phytophthora camphora (Phytophthora cinnaomi Rands) through separation, purification and identification in a laboratory, and is stored on a PDA culture medium.
PDA culture medium: 200g of peeled potatoes, 20g of glucose, 20g of agar and 1000mL of water, and the pH value is natural.
Test method (refer to 'NY/T1156.2-2006 indoor bioassay standard for agricultural chemicals bactericide part 2: inhibition of pathogenic fungi hypha growth test plate method')
1. The test reagent is dissolved by dimethyl sulfoxide, and then diluted by 0.1% Tween-80 to prepare single-dose mother liquor, and multiple groups of ratios are set, and 5 gradient mass concentrations of each single dose and each group of mixture are set according to an equal ratio method.
2. Adding the pre-melted PDA culture medium into a sterile conical flask, quantitatively sucking liquid medicine from low concentration to high concentration in sequence, adding into the above conical flasks respectively, and shaking thoroughly. Then, the mixture was poured into 4 dishes 9cm in diameter in equal amounts to prepare drug-containing plates, and blank controls containing no active ingredient were added, and 3 replicates were set for each treatment.
3. And (3) punching holes on the edges of the bacterial colonies of the tested germs by using a sterilization puncher with the diameter of 5mm to prepare bacterial cakes with the diameter of 5 mm. Inoculating the fungus cake in the center of the medicated plate with the hypha facing upwards, covering with a dish, and culturing at 25 deg.C in an incubator.
4. When the diameter of the blank control bacterial colony is more than 2/3 of the diameter of the culture dish, measuring the diameter of the bacterial colony by a caliper, vertically measuring the diameter of each bacterial colony by a cross method once, taking the average value, calculating the hypha growth inhibition rate of each treatment, performing regression analysis on the agent concentration logarithm value and the hypha growth inhibition rate probability value by DPS software, and calculating the EC of each treatment agent50And calculating the mixture according to the Sun cloud Peel methodCo-toxicity coefficient (CTC value) of the agent.
D＝D1-D2
In the above formula: d- -colony growth diameter; d1-colony diameter; d2- -the diameter of the cake.
In the above formula: i- -hypha inhibition rate; d0-blank control colony growth diameter; dtAgent-treated colony growth diameter.
Measured virulence index (ATI) ═ standard agent EC50Reagent EC for test50)×100；
Theoretical virulence index (TTI) ═ a agent virulence index x percent of a in the mixture + B agent virulence index x percent of B in the mixture;
co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].
Evaluating the compounding of the medicament according to the co-toxicity coefficient: CTC is less than or equal to 80 as antagonism; 80 < CTC < 120 is additive effect; CTC is more than or equal to 120, which is a synergistic effect. The results are shown in tables 1 to 3.
TABLE 1 results of indoor bioactivity assay of fluthiazopyridone and triadimenol combination on Phytophthora cinnamomi
| Name and proportion of the medicament | EC50(mg/L) | ATI | TTI | CTC |
| Fluroxyphiazolopyridinone | 1.07 | 100.00 | -- | -- |
| Triazole alcohols | 4.24 | 25.06 | -- | -- |
| Fluroxothiazolopyrietophenone 1: triadimenol 40 | 2.46 | 43.50 | 26.89 | 161.78 |
| Fluroxothiazolopyrietophenone 1: triadimenol 20 | 1.34 | 79.85 | 28.63 | 278.93 |
| Fluroxothiazolopyrietophenone 1: triadimenol 10 | 0.75 | 142.67 | 31.87 | 447.63 |
| Fluthiazolepyrietone 1: triadimenol 5 | 0.29 | 368.97 | 37.55 | 982.63 |
| Fluroxothiazolopyrietophenone 1: triadimenol 1 | 1.12 | 95.54 | 62.53 | 152.79 |
| Fluoro thiazole pyriethanone 5: triadimenol 1 | 0.90 | 118.89 | 87.51 | 135.86 |
| 10 parts of fluorothiazole pyrithylone: triadimenol 1 | 0.64 | 167.19 | 93.19 | 179.41 |
| 30 parts of oxathiapiprolin: triadimenol 1 | 0.42 | 254.76 | 97.58 | 261.07 |
| 50 parts of oxathiapiprolin: triadimenol 1 | 0.56 | 191.07 | 98.53 | 193.92 |
As can be seen from Table 1, the mass ratio of the fluorothiazole pyrithylone to the triadimenol is 1-50: the cotoxicity coefficients within the range of 40-1 are all larger than 120, which shows that the compounded compound of the fluorothiazole pyrithylone and the triadimenol has a synergistic effect on the phytophthora cinnamomi. Especially when the mass ratio is 1: and 5, the co-toxicity coefficient reaches 982.63, and the synergistic effect is most obvious.
TABLE 2 results of indoor bioactivity assay of pythium camphorate with fluthiazopyr and mepiquat chloride
| Name and proportion of the medicament | EC50(mg/L) | ATI | TTI | CTC |
| Fluroxyphiazolopyridinone | 1.07 | 100.00 | -- | -- |
| Methyrachlor ester | 6.19 | 17.29 | -- | -- |
| Fluroxothiazolopyrietophenone 1: methyrachlor 20 | 3.54 | 30.23 | 21.22 | 142.41 |
| Fluthiazolepyrietone 1: methyrachlor 10 | 2.19 | 48.86 | 24.81 | 196.97 |
| Fluroxothiazolopyrietophenone 1: methyrachlor 5 | 1.59 | 67.30 | 31.07 | 216.58 |
| Fluroxothiazolopyrietophenone 1: methyrachlor 1 | 1.07 | 100.00 | 58.64 | 170.52 |
| Fluoro thiazole pyriethanone 2: methyrachlor 1 | 0.57 | 187.72 | 72.43 | 259.18 |
| Fluoro thiazole pyriethanone 4: methyrachlor 1 | 0.24 | 445.83 | 83.46 | 534.21 |
| Fluroxothiazolopyrietophenone 8: methyrachlor 1 | 0.82 | 130.49 | 90.81 | 143.69 |
As can be seen from Table 2, the mass ratio of the fluorothiazole pyrithylone to the mefenacet is 1-8: the cotoxicity coefficients within the range of 20-1 are all larger than 120, which shows that the compounded compound of the fluorothiazole pyrithylone and the methyl benzoate has a synergistic effect on the phytophthora cinnamomi.
TABLE 3 Compound pair of Fluroxypyrazolopyrlether and fenhexamid Phytophthora camphora indoor bioactivity determination results
As can be seen from Table 3, the weight ratio of the fluorothiazole pyrithylone to the fenhexamid is 1-5: the co-toxicity coefficients within the range of 16-1 are all larger than 120, which shows that the compounded compound of the fluorothiazole pyrithylone and the fenhexamid has a synergistic effect on the phytophthora cinnamomi.
In conclusion, when the fluorothiazole pyrithylone, the triadimenol, the methyl benzoate or the fenhexamid are compounded, a synergistic effect is shown in a certain mass ratio range, and the control effect on the macadamia nut stem canker caused by phytophthora camphora infection can be improved.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (5)
1. The macadimia nut stem canker inhibitor based on a molecular disease-resistant approach is characterized in that the effective components of the inhibitor are compounded by fluorothiazole pyrithylone, triadimenol, and metrafoxanil or fenhexamid.
2. The macadimia nut stem canker disease inhibitor according to claim 1, wherein the mass ratio of the fluorothiazolopyrone to the triadimenol is 1-50: 40-1.
3. The macadimia nut stem canker disease inhibitor according to claim 2, wherein the mass ratio of the fluorothiazolopyrone to the triadimenol is 1: 5.
4. the macadimia nut stem canker disease inhibitor according to claim 1, wherein the mass ratio of the fluorothiazolepyrithylone to the mefenamate is 1-8: 20-1.
5. The macadimia nut stem canker inhibitor according to claim 1, wherein the mass ratio of the fluorothiazolopyrone to the fenhexamid is 1-5: 16-1.
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| CN114988958A (en) * | 2022-07-12 | 2022-09-02 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Pharmaceutical composition for preventing and treating macadamia nut recession disease |
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