CN114190394B - Fruit black spot resistant activator for macadimia nut breeding - Google Patents
Fruit black spot resistant activator for macadimia nut breeding Download PDFInfo
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- CN114190394B CN114190394B CN202111659417.5A CN202111659417A CN114190394B CN 114190394 B CN114190394 B CN 114190394B CN 202111659417 A CN202111659417 A CN 202111659417A CN 114190394 B CN114190394 B CN 114190394B
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- black spot
- macadimia nut
- reserpine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to the technical field of pesticide research and development, and particularly relates to a fruit black spot resistant activator for macadimia nut breeding. An activator for resisting fruit black spot for macadimia nut breeding comprises reserpine, isopyrazam, penoxsulam or mandipropamid. The fruit black spot resistant activator of the invention shows good synergy; can improve the control effect on black spot of macadimia nut fruits, reduce the application dosage of pesticides and reduce pesticide residues.
Description
Technical Field
The invention belongs to the technical field of pesticide research and development, and particularly relates to a fruit black spot resistant activator for macadimia nut breeding.
Background
Macadimia nut, the alias Xia Weini fruit, is a native plant of the genus macadimia, which has high economic value, good nutritional value and medicinal value, and is a reputation of "king dried fruits". In the planting process of the macadimia nut, black spots appear on the fruits when the fruits are infected by the aschersonia aleyrodis, black round spots appear on the fruits at the early stage of disease occurrence, white hyphae and conidia can appear on the spots when the spots are serious, and the yield and quality of the macadimia nut are affected. Spraying chemical pesticides is a common means for preventing and controlling black spot of macadimia nut fruits, but long-term use of a single chemical pesticide can cause generation of disease resistance, so that the prevention and control effect of the chemical pesticide on the black spot of macadimia nut fruits is reduced.
Reserpine is an indole alkaloid with chemical formula of C 33 H 40 N 2 O 9 Colorless crystals, readily soluble in chloroform, methylene chloride and glacial acetic acid. Clinically, reserpine can reduce blood pressure and slow heart rate, has slow, mild and durable action, has durable and stable action on the central nervous system, and is a good sedative. Researches show that reserpine has remarkable synergism on bactericides with different action mechanisms, and application number CN201810979216.5 discloses application of reserpine as a bactericide synergist in plant gray mold control.
The isopyrazam is a bactericide active ingredient which is researched and developed by the Zhengda company and has the functions of preventing and treating, belongs to an SDHI bactericide, can influence the respiration of pathogenic fungi by inhibiting succinic dehydrogenase in tricarboxylic acid circulation in the respiration process of pathogenic fungi, can block energy metabolism, can inhibit the growth of the pathogenic fungi, can cause the death of the pathogenic fungi, and can be used for preventing common leaf spot, brown rust, yellow rust and the like in crops.
The fenhexamid is a novel-structure bactericide which is independently researched and developed by Shenyang chemical institute, has wide bactericidal spectrum and high antibacterial activity, and has good inhibition effect on plant pathogenic fungi and oomycetes. It is reported that after the exogenous addition of ATP to the culture medium with drugs, the inhibition rate of the fenhexamid on phytophthora capsici silk growth, zoospore release and telogen germination stage is remarkably reduced, and the action mechanism of the fenhexamid is presumably related to energy metabolism and ATP synthesis.
The mandipropamid is an amide bactericide, and has the action mechanism of inhibiting biosynthesis of phospholipid and high prevention effect on most leaf and fruit diseases caused by oomycetes. Has high activity on spores in germination stage, and can inhibit hypha growth and sporulation. In addition, the plant leaf can be quickly absorbed by the leaf and stays in the waxy layer of the leaf surface, so that the leaf is protected.
The inventor researches show that when reserpine is compounded with isopyrazam, bixafen or mandipropamid, the synergistic effect is shown in a certain mass ratio range, the prevention and treatment effect on black spot of macadimia nut fruits can be improved, and no related report of the combination is found at present.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide an anti-fruit black spot activator for macadimia nut breeding, which can improve the control effect of the macadimia nut fruit black spot and provide help for developing a novel bactericide.
In order to achieve the aim, the invention provides an anti-fruit black spot activator for macadimia nut breeding, which is prepared by compounding reserpine and isopyrazam, dimefon or mandipropamid.
Preferably, the mass ratio of the reserpine to the isopyrazam is 1-30:5-1.
Preferably, the mass ratio of the reserpine to the dimoxystrobin is 1-10:8-1.
Preferably, the mass of the reserpine and the mandipropamid is 1-6:20-1.
Compared with the prior art, the invention has the following beneficial effects:
the fruit black spot resistant activator provided by the invention has a good synergistic effect, can improve the control effect on the black spot of the macadimia nut fruits, can reduce the application dosage of pesticides and can reduce the pesticide residues.
Detailed Description
The following detailed description of specific embodiments of the invention is, but it should be understood that the invention is not limited to specific embodiments.
Examples: indoor biological activity test after reserpine compounding
Test agent: reserpine (Shanghai Ala Biotechnology Co., ltd.), isopyrazam 92%, penoxsulam 98%, mandipropamid 93%.
Test pathogenic bacteria: picking macadimia nut fruits infected by a pathogen from an orchard, separating and purifying in a laboratory, and identifying the pathogen as the aschersonia aleyrodis.
The test method comprises the following steps:
1. dissolving the raw materials with dimethyl sulfoxide, diluting with 0.1% Tween-80 to obtain single-dose mother solution, and setting multiple groups of ratios, wherein each single dose and each group of ratio mixture have 6 gradient mass concentrations according to an equal ratio method for use.
2. Under aseptic condition, the pre-melted PDA culture medium is quantitatively added into an aseptic conical flask, the liquid medicine is quantitatively sucked from low concentration to high concentration sequentially, and the liquid medicine is respectively added into the conical flask and fully shaken. Then, the mixture was poured into 4 dishes having a diameter of 9cm in equal amounts to prepare a medicated plate, and a blank treatment containing only dimethyl sulfoxide and 0.1% Tween-80 was set for each treatment, and 3 replicates were obtained.
3. Under the aseptic condition, a sterilization puncher with the diameter of 5mm is used for punching holes on the edges of bacterial colonies to prepare bacterial cakes with the diameter of 5 mm; the bacterial cake is inoculated in the center of the drug-containing flat plate by an inoculator, the mycelium surface faces upwards, and the bacterial cake is placed in an incubator at 25 ℃ for culture after the dish cover is covered.
4. When the diameter of the blank control colony reaches more than 2/3 of the diameter of the culture dish, measuring the colony diameter by a caliper, vertically measuring the diameter of each colony by a crisscross method, taking the average value of the colony, calculating the growth inhibition rate of hyphae of each treatment, carrying out regression analysis on the logarithmic value of the concentration of the medicament and the value of the growth inhibition rate of the hyphae by DPS software, calculating the EC50 of each treatment medicament, and calculating the co-toxicity coefficient (CTC value) of the mixture according to a grand cloud Peel method.
Colony growth diameter = D 1 -D 2 ，D 1 For colony diameter, D 2 Is a cake diameter (in this example, cake diameter is 5 mm);
hypha growth inhibition (%) = [ (D) 0 -D t )/D 0 ]×100，D 0 Growing the diameter of a blank control colony; d (D) t Colony growth diameter for agent treatment;
measured virulence index (ATI) = (standard agent EC 50 Reagent EC 50 )×100；
Theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture;
co-toxicity coefficient (CTC) = [ actual drug susceptibility index (ATI)/theoretical drug susceptibility index (TTI) ] x 100.
Dividing the standard according to the joint action: the co-toxicity coefficient (CTC) is more than or equal to 120, and the synergistic effect is shown; the cotoxicity coefficient (CTC) is less than or equal to 80 and shows antagonism; 80< co-toxicity coefficient (CTC) <120 shows additive effect. The experimental results are shown in tables 1-3.
TABLE 1 indoor biological Activity determination of reserpine and isopyrazam on Australian nut fruit Spot pathogen
As can be seen from table 1, after reserpine and isopyrazam are compounded, the mass ratio is 1-30: the co-toxicity coefficient of the macadimia nut fruit black spot pathogenic bacteria is more than 120 in the range of 5-1, and the synergistic effect is shown.
TABLE 2 indoor biological Activity determination of reserpine and Bifenamid Complex against Australian nut fruit Spot disease pathogen
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Reserpine for promoting blood circulation | 39.15 | 100.00 | -- | -- |
| Bifenamid | 8.32 | 470.55 | -- | -- |
| Reserpine 1: bifenamid 8 | 6.84 | 572.37 | 429.38 | 133.30 |
| Reserpine 1: bifenamid 5 | 2.53 | 1547.43 | 408.79 | 378.54 |
| Reserpine 1: bifenamid 2 | 1.26 | 3107.14 | 347.04 | 895.34 |
| Reserpine 1: bifenamid 1 | 2.82 | 1388.30 | 285.28 | 486.65 |
| Reserpine 2: bifenamid 1 | 3.47 | 1128.24 | 233.52 | 504.77 |
| Reserpine 5: bifenamid 1 | 6.43 | 608.86 | 161.76 | 376.40 |
| Reserpine 8: bifenamid 1 | 7.27 | 538.51 | 141.17 | 381.46 |
| Reserpine 10: bifenamid 1 | 4.49 | 871.84 | 133.69 | 652.23 |
As can be seen from table 2, after reserpine and dimoxystrobin are compounded, the mass ratio is 1-10: in the range of 8-1, the co-toxicity coefficient of the black spot pathogen of the macadimia nut fruits is more than 120, and the synergistic effect is shown.
TABLE 3 indoor biological Activity determination of reserpine and mandipropamid combinations against Australian nut fruit Spot disease pathogen
As can be seen from table 3, after reserpine and mandipropamid are compounded, the mass ratio is 1-6: the co-toxicity coefficient of the black spot pathogenic bacteria of the macadimia nut fruits is more than 120 in the range of 20-1, and the synergistic effect is shown.
In conclusion, after the reserpine and the isopyrazam, the bixafen or the mandipropamid are compounded, the reserpine and the mandipropamid are synergistic in a certain mass ratio range to pathogenic bacteria of the black spot of the macadimia nut fruits, and the control effect on the black spot of the macadimia nut fruits can be improved.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (1)
1. The fruit black spot resistant activator for macadimia nut breeding is characterized in that the active ingredients of the fruit black spot resistant activator are compounded by reserpine and isopyrazam; the mass ratio of reserpine to isopyrazam is 1-30:5-1.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310129669.XA CN117530282A (en) | 2021-12-30 | 2021-12-30 | Prevention and treatment agent for fruit black spot |
| CN202111659417.5A CN114190394B (en) | 2021-12-30 | 2021-12-30 | Fruit black spot resistant activator for macadimia nut breeding |
| CN202310129671.7A CN116138262A (en) | 2021-12-30 | 2021-12-30 | Bactericide for preventing and treating fruit black spot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202111659417.5A CN114190394B (en) | 2021-12-30 | 2021-12-30 | Fruit black spot resistant activator for macadimia nut breeding |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202310129671.7A Division CN116138262A (en) | 2021-12-30 | 2021-12-30 | Bactericide for preventing and treating fruit black spot |
| CN202310129669.XA Division CN117530282A (en) | 2021-12-30 | 2021-12-30 | Prevention and treatment agent for fruit black spot |
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| CN114190394A CN114190394A (en) | 2022-03-18 |
| CN114190394B true CN114190394B (en) | 2023-05-26 |
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| CN202310129669.XA Pending CN117530282A (en) | 2021-12-30 | 2021-12-30 | Prevention and treatment agent for fruit black spot |
| CN202310129671.7A Pending CN116138262A (en) | 2021-12-30 | 2021-12-30 | Bactericide for preventing and treating fruit black spot |
| CN202111659417.5A Active CN114190394B (en) | 2021-12-30 | 2021-12-30 | Fruit black spot resistant activator for macadimia nut breeding |
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| CN202310129669.XA Pending CN117530282A (en) | 2021-12-30 | 2021-12-30 | Prevention and treatment agent for fruit black spot |
| CN202310129671.7A Pending CN116138262A (en) | 2021-12-30 | 2021-12-30 | Bactericide for preventing and treating fruit black spot |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| NZ708588A (en) * | 2012-11-19 | 2019-03-29 | Arch Wood Protection Inc | Succinate dehydrogenase inhibitor containing compositions |
| GB201710057D0 (en) * | 2017-06-23 | 2017-08-09 | Univ Southampton | Coating |
| CN108739835B (en) * | 2018-08-27 | 2021-04-09 | 中国农业大学 | Application of reserpine as bactericidal synergist in prevention and treatment of plant gray mold |
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- 2021-12-30 CN CN202310129669.XA patent/CN117530282A/en active Pending
- 2021-12-30 CN CN202310129671.7A patent/CN116138262A/en active Pending
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| CN114190394A (en) | 2022-03-18 |
| CN116138262A (en) | 2023-05-23 |
| CN117530282A (en) | 2024-02-09 |
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Effective date of registration: 20230418 Address after: 251600 Huiyuan street, Shanghe Economic Development Zone, Jinan City, Shandong Province Applicant after: Shandong Longxi Plant Co.,Ltd. Address before: Yong Wu, 530001 Nanning Road, the Guangxi Zhuang Autonomous Region No. 22 Applicant before: GUANGXI SUBTROPICAL CROPS Research Institute (GUANGXI SUBTROPICAL AGRICULTURAL PRODUCTS PROCESSING RESEARCH INSTITUTE) |
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Denomination of invention: An Activator for Fruit Black Spot Resistance in Macadamia Nut Breeding Effective date of registration: 20230829 Granted publication date: 20230526 Pledgee: Shandong Shanghe Rural Commercial Bank Co.,Ltd. Taishangcheng Branch Pledgor: Shandong Longxi Plant Co.,Ltd. Registration number: Y2023980054388 |
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