CN112075456B

CN112075456B - Biological pesticide for preventing and treating macadamia nut flower blight

Info

Publication number: CN112075456B
Application number: CN202010976400.1A
Authority: CN
Inventors: 蔡元保; 杨祥燕; 李季东; 李穆; 黄思婕; 巫辅民; 曾黎明; 林玉虹; 庞新华; 郑文武; 潘如军; 黄锦媛
Original assignee: Guangxi Subtropical Crops Research Institute
Current assignee: Guangxi Subtropical Crops Research Institute
Priority date: 2020-09-16
Filing date: 2020-09-16
Publication date: 2021-06-01
Anticipated expiration: 2040-09-16
Also published as: CN112075456A

Abstract

The invention belongs to the field of pesticides, and particularly relates to a biological pesticide for preventing and treating macadimia nut flower blight and application thereof. The active ingredients of the biological pesticide for preventing and treating the macadimia nut blight comprise an active ingredient A and an active ingredient B, wherein the active ingredient A is a 1% shenqinmycin suspending agent, and the active ingredient B is a bacillus licheniformis aqueous solution with 80 hundred million live spores per milliliter. The biological pesticide has good control effect on the macadimia nut flower blight, and the control effect is 90.5% and 87.3% respectively when the concentration is 300 mg/L.

Description

Biological pesticide for preventing and treating macadamia nut flower blight

Technical Field

The invention belongs to the field of pesticides, and particularly relates to a biological pesticide for preventing and treating macadimia nut flower blight and application thereof.

Background

The macadimia nut blight is caused by botrytis fungi, infected parts are all arranged at the front ends of inflorescences, the infected parts are browned and withered, and a layer of grayish green mildew-shaped substances (conidia and conidiophores) are wrapped on the surfaces of the infected parts. Macadimia nuts are easy to infect the diseases of the flower blight in the whole flowering phase, and the diseases are more aggravated in low-temperature and foggy weather. The invention aims to develop a biological agent for preventing and treating macadimia nut flower blight.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a biological pesticide for preventing and treating macadimia nut flower blight and application thereof.

The technical scheme provided by the invention is as follows:

the active ingredients of the biological pesticide for preventing and treating the macadimia nut blight comprise an active ingredient A and an active ingredient B, wherein the active ingredient A is a 1% shenqinmycin suspending agent, and the active ingredient B is a bacillus licheniformis aqueous solution with 80 hundred million live spores per milliliter.

The mass ratio of the active ingredient A to the active ingredient B of the biological pesticide is 10:1-1: 10.

Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:1-1: 5.

The invention also provides application of the biological pesticide in preventing and treating macadimia nut flower blight.

Compared with the prior art, the invention has the following beneficial effects:

(1) according to the invention, the shenqinmycin and the bacillus licheniformis with different proportions have different effects on the bacterial blight of the macadimia nut, and are expressed as synergistic and additive effects; when the ratio of the 1% shenqinmycin suspending agent to 80 hundred million live spores per milliliter of bacillus licheniformis water agent is 1:1 and 1:5, the synergistic effect is shown.

(2) The biological pesticide has good control effect on the macadimia nut flower blight, and the control effect is 90.5% and 87.3% respectively when the concentration is 300 mg/L.

Detailed Description

The invention will be better understood from the following examples. However, those skilled in the art will readily appreciate that the description of the embodiments is only for illustrating the present invention and should not be taken as limiting the invention as detailed in the claims.

Example 1: determination of toxicity of shenqinmycin and bacillus licheniformis to bacterial blight of macadamia nut

Test germs: bacterial blight of macadamia ternifolia; collecting macadimia nut susceptible spica from the field, inoculating the macadimia nut susceptible spica on a PDA (personal digital Assistant) plate after surface disinfection, and preserving for later use after culture and identification.

Reagent to be tested: 1% of a shenqinmycin suspending agent and a bacillus licheniformis aqueous agent (80 hundred million live spores per milliliter); the above-mentioned medicaments are all commercially available.

The test method comprises the following steps: a growth rate method (plate method) is adopted according to the agricultural industry standard of the people's republic of China. On the basis of a preliminary test, quantitatively adding a pre-melted sterile culture medium into a sterile conical flask according to test treatment, sequentially adding 5mL of liquid medicine into conical flasks filled with 45mL of hot culture medium from low concentration to high concentration, shaking uniformly, quickly pouring the culture medium into glass culture dishes with the diameter of 90mm when the culture medium is reduced to a proper temperature, and pouring 10mL of culture medium with the medicine into each culture dish. Standing horizontally, cooling, and making into flat plate. Each concentration was 4 replicates. The blank control was performed with treatment containing no active ingredient. Cutting the cultured pathogenic bacteria from the edge of the bacterial colony by using a sterilization puncher with the diameter of 4mm under aseptic conditions, inoculating the bacterial cake to the center of a drug-containing flat plate by using an inoculator, attaching the side with the hyphae downwards to a culture medium, and covering a dish cover. All the operations are carried out in a super clean bench for sterile operation. And culturing in a constant-temperature sterile incubator for 4 days, and taking out. The colony diameters of the treatments were measured by the cross method, and the average of the colony diameters was calculated. And calculating the net growth amount and the hypha growth inhibition rate of each treatment hypha.

The net growth (mm) is measured as the colony diameter-4;

the hypha growth inhibition ratio (%) (blank colony growth diameter-agent-treated colony growth diameter)/blank colony growth diameter × 100.

Regression analysis was performed based on the number of inhibition rate of hypha growth (y) and the logarithm of the concentration of the drug solution (x), the inhibitory intermediate concentration EC50 was calculated, the synergistic coefficient was calculated based on the Wadley method, and the synergistic effect was evaluated.

TABLE 1 different ratios of shenqinmycin and Bacillus licheniformis

Calculating the synergistic coefficient SR of different medicament ratios by using a Wadley method, wherein the SR is more than or equal to 1.5, which shows that the synergistic effect is achieved; SR < 0.5 shows antagonistic action; an additive effect is that SR is more than 0.5 and less than 1.5.

TABLE 2 Combined assay of the effects of shenqinmycin and Bacillus licheniformis on macadamia nut flower blight

Remarking: wherein mg/L is the concentration of the preparation.

As can be seen from Table 2, different proportions of shenqinmycin and Bacillus licheniformis have different effects on the bacterial blight of macadimia nut, and the effects are shown as synergistic and additive effects; when the ratio of the 1% of the shenqinmycin suspending agent to 80 hundred million live spores per milliliter of the bacillus licheniformis water agent is 1:1 and 1:5, the synergistic effect is shown; the rest proportion is additive effect.

Example 2: determination of field drug effect of shenqinmycin and bacillus licheniformis on macadamia nut flower blight

Test subjects: macadamia nut flower blight.

The test method comprises the following steps: the experiment was set up with 5 treatments, each of which was repeated 3 times. Spray treatment is adopted before macadimia nuts bloom, 50 macadimia nut spiles are randomly selected in each treatment, disease indexes are investigated 30 days after the macadimia nuts bloom (in the flowering phase), and control effects are calculated.

The calculation formula of disease index and relative prevention and treatment effect is as follows:

disease index is 100 × Σ (number of diseased leaves at each stage × representative value at each stage)/(total number of examined leaves × representative value at highest stage);

relative prevention and treatment effect (%) (control disease index-treatment disease index)/control disease index × 100.

The classification standard of the disease condition of the macadimia nut blight is as follows:

level 0: no susceptibility to diseases;

level 1: 1/5 diseased flower part of the whole flower spike;

and 2, stage: 2/5 diseased flower part of the whole flower spike;

and 3, level: 3/5 diseased flower part of the whole flower spike;

4, level: 4/5 diseased flower part of the whole flower spike;

and 5, stage: the whole panicle is infected with diseases.

The test results are shown in Table 3.

TABLE 3 field pharmacodynamic assay of shenqinmycin and Bacillus licheniformis on macadamia nut flower blight

As can be seen from Table 3, the complex combination of shenqinmycin and Bacillus licheniformis has good control effect on the flower blight of macadamia nut; the control effect is 90.5 percent and 87.3 percent respectively when the concentration is 300 mg/L.

The foregoing is only a preferred embodiment of the present invention, and it will be apparent to those skilled in the art that modifications may be made without departing from the invention as claimed in the invention and these modifications are within the scope of the invention.

Claims

1. The biological pesticide for preventing and treating the macadimia nut blight is characterized in that active ingredients comprise an active ingredient A and an active ingredient B, wherein the active ingredient A is a 1% shenqinmycin suspending agent, and the active ingredient B is 80 hundred million live spores/milliliter of a bacillus licheniformis aqueous solution; the mass ratio of the active component A to the active component B is 1:1-1: 5.

2. Use of the biopesticide of claim 1 for controlling macadimia nut blight.