CN114467950B - Sterilization composition for preventing and treating taro epidemic disease - Google Patents
Sterilization composition for preventing and treating taro epidemic disease Download PDFInfo
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- CN114467950B CN114467950B CN202210139113.4A CN202210139113A CN114467950B CN 114467950 B CN114467950 B CN 114467950B CN 202210139113 A CN202210139113 A CN 202210139113A CN 114467950 B CN114467950 B CN 114467950B
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- taro
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- fluxapyroxad
- metalaxyl
- cyazofamid
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- 244000205754 Colocasia esculenta Species 0.000 title claims abstract description 30
- 235000006481 Colocasia esculenta Nutrition 0.000 title claims abstract description 30
- 239000000203 mixture Substances 0.000 title claims abstract description 27
- 201000010099 disease Diseases 0.000 title claims abstract description 24
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 24
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 11
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 11
- 239000005788 Fluxapyroxad Substances 0.000 claims abstract description 38
- SXSGXWCSHSVPGB-UHFFFAOYSA-N fluxapyroxad Chemical compound FC(F)C1=NN(C)C=C1C(=O)NC1=CC=CC=C1C1=CC(F)=C(F)C(F)=C1 SXSGXWCSHSVPGB-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000005807 Metalaxyl Substances 0.000 claims abstract description 28
- ZQEIXNIJLIKNTD-UHFFFAOYSA-N methyl N-(2,6-dimethylphenyl)-N-(methoxyacetyl)alaninate Chemical compound COCC(=O)N(C(C)C(=O)OC)C1=C(C)C=CC=C1C ZQEIXNIJLIKNTD-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000005754 Cyazofamid Substances 0.000 claims abstract description 24
- YXKMMRDKEKCERS-UHFFFAOYSA-N cyazofamid Chemical compound CN(C)S(=O)(=O)N1C(C#N)=NC(Cl)=C1C1=CC=C(C)C=C1 YXKMMRDKEKCERS-UHFFFAOYSA-N 0.000 claims abstract description 23
- QNBTYORWCCMPQP-JXAWBTAJSA-N (Z)-dimethomorph Chemical compound C1=C(OC)C(OC)=CC=C1C(\C=1C=CC(Cl)=CC=1)=C/C(=O)N1CCOCC1 QNBTYORWCCMPQP-JXAWBTAJSA-N 0.000 claims abstract description 14
- 239000005761 Dimethomorph Substances 0.000 claims abstract description 14
- GOFJDXZZHFNFLV-UHFFFAOYSA-N 5-fluoro-1,3-dimethyl-N-[2-(4-methylpentan-2-yl)phenyl]pyrazole-4-carboxamide Chemical compound CC(C)CC(C)C1=CC=CC=C1NC(=O)C1=C(F)N(C)N=C1C GOFJDXZZHFNFLV-UHFFFAOYSA-N 0.000 claims description 4
- 239000005815 Penflufen Substances 0.000 claims description 4
- 244000052616 bacterial pathogen Species 0.000 abstract description 14
- 239000003814 drug Substances 0.000 abstract description 14
- 230000002195 synergetic effect Effects 0.000 abstract description 12
- 241000233614 Phytophthora Species 0.000 abstract description 11
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 10
- 239000000575 pesticide Substances 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 7
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 239000004480 active ingredient Substances 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000000447 pesticide residue Substances 0.000 abstract description 3
- 206010034133 Pathogen resistance Diseases 0.000 abstract description 2
- 230000003111 delayed effect Effects 0.000 abstract description 2
- 230000007246 mechanism Effects 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 description 8
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 7
- 230000004071 biological effect Effects 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- ZMYFCFLJBGAQRS-IRXDYDNUSA-N (2R,3S)-epoxiconazole Chemical compound C1=CC(F)=CC=C1[C@@]1(CN2N=CN=C2)[C@H](C=2C(=CC=CC=2)Cl)O1 ZMYFCFLJBGAQRS-IRXDYDNUSA-N 0.000 description 4
- 239000005767 Epoxiconazole Substances 0.000 description 4
- 239000013043 chemical agent Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000001018 virulence Effects 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 239000005605 Pyraflufen-ethyl Substances 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- APTZNLHMIGJTEW-UHFFFAOYSA-N pyraflufen-ethyl Chemical group C1=C(Cl)C(OCC(=O)OCC)=CC(C=2C(=C(OC(F)F)N(C)N=2)Cl)=C1F APTZNLHMIGJTEW-UHFFFAOYSA-N 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000004563 wettable powder Substances 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000626603 Phytophthora colocasiae Species 0.000 description 2
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 2
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 2
- 125000002252 acyl group Chemical group 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- RFHAOTPXVQNOHP-UHFFFAOYSA-N fluconazole Chemical compound C1=NC=NN1CC(C=1C(=CC(F)=CC=1)F)(O)CN1C=NC=N1 RFHAOTPXVQNOHP-UHFFFAOYSA-N 0.000 description 2
- 229960004884 fluconazole Drugs 0.000 description 2
- 244000053095 fungal pathogen Species 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000209524 Araceae Species 0.000 description 1
- 235000005273 Canna coccinea Nutrition 0.000 description 1
- 240000008555 Canna flaccida Species 0.000 description 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 229940124186 Dehydrogenase inhibitor Drugs 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- YIKSCQDJHCMVMK-UHFFFAOYSA-N Oxamide Chemical compound NC(=O)C(N)=O YIKSCQDJHCMVMK-UHFFFAOYSA-N 0.000 description 1
- 241001149949 Phytophthora cactorum Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000005842 Thiophanate-methyl Substances 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000006540 mitochondrial respiration Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical compound COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/84—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and treating taro epidemic disease. The active ingredients of the sterilization composition are compounded by fluxapyroxad, cyazofamid, dimethomorph or metalaxyl. The sterilization mechanisms of the epoxiconazole-oxammonium, the cyazofamid, the dimethomorph or the metalaxyl in the sterilization composition are different, so that the generation of drug resistance of pathogenic bacteria of the taro epidemic disease can be delayed, and the service life of the sterilization composition can be prolonged. In addition, when the 2 effective components in the bactericidal composition are compounded according to a certain mass ratio, the bactericidal composition has no cross resistance and shows better synergistic effect. Compared with a single active ingredient, the composition can improve the control effect on the phytophthora blight of the taro, is beneficial to reducing the application dosage of pesticide, reduces pesticide residue and control cost, and can provide support for developing novel medicaments.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and treating taro epidemic disease.
Background
Taro belongs to the genus taro of the family Araceae, is a perennial herb, has a bulb rich in various nutritional ingredients such as starch, vitamins and amino acids, and is a widely cultivated vegetable and grain crop worldwide. Guangxi has become one of the main producing areas of the taros in China at present.
Taro epidemic disease is one of the main diseases in taro production, and mainly damages leaves, petioles and bulbs. At the beginning of disease, the leaves appear yellow brown round spots, then gradually expand, have obvious concentric rings with alternating density, and have dark green water stain-shaped rings at the edges. Several lesions are usually healed into irregular shapes, and the central part of the lesion is rotten and perforated later, and in severe cases, only veins are remained. The research shows that the taro epidemic disease is caused by the infection of Phytophthora taro (Phytophthora colocasiae) of the subdivision flagellata.
At present, chemical agents are main means for preventing and treating the taro epidemic disease, and common chemical agents comprise 25% metalaxyl wettable powder, 50% carbendazim wettable powder, 70% thiophanate-methyl wettable powder and the like, but the taro epidemic disease has different-course drug resistance to a plurality of existing chemical agents due to long-term use of single agents for prevention and treatment. In order to improve the control effect, the application dosage of the pesticide is increased, so that the generation of drug resistance is further accelerated, the control cost is increased, and the pesticide residue is increased. Therefore, there is a need to research and develop new drugs.
The fluxapyroxad is a latest generation succinate dehydrogenase inhibitor bactericide discovered and developed by promptures, influences the respiration of pathogenic fungi by inhibiting succinate dehydrogenase in tricarboxylic acid cycle in the mitochondrial respiration of pathogenic bacteria, hinders energy metabolism, inhibits the growth of the pathogenic bacteria, causes the death of the pathogenic bacteria, and has the advantages of high efficiency, broad spectrum, low toxicity and the like. Application number CN201910756614.5 discloses a pesticide composition containing pyraflufen-ethyl and application thereof, and particularly discloses that the pyraflufen-ethyl and amino-oligosaccharin are compounded according to a certain mass ratio to have remarkable control effects on wheat scab and tomato late blight.
Compounding different pesticide active ingredients is a common means for developing new pesticide products and preventing and treating resistant diseases and insect pests at present, and the compounded pesticide can show the following characteristics: the compound medicament with the synergistic effect can effectively improve the control effect on the plant diseases and insect pests and delay the generation of drug resistance of the plant diseases and insect pests compared with a single medicament. The inventor discovers that after the fluxapyroxad and cyazofamid, dimethomorph or metalaxyl are compounded according to a certain mass ratio, the compound has a good synergistic effect on the pathogenic bacteria of the taro blight, and supports can be provided for developing novel medicaments based on the synergistic effect.
Disclosure of Invention
The invention aims to provide a bactericidal composition for preventing and treating taro epidemic disease, which solves the problem of long-term use of a single chemical agent.
In order to realize the purpose, the invention provides the following technical scheme:
the invention aims to provide a bactericidal composition, the active ingredients of which are compounded by fluxapyroxad and cyazofamid, dimethomorph or metalaxyl.
Preferably, the mass ratio of the penflufen to the cyazofamid is 1-3:25-1.
Preferably, the mass ratio of the penflufen to the dimethomorph is 1-10:5-1.
Preferably, the mass ratio of the fluxapyroxad to the metalaxyl is 1-30:40-1.
The second purpose of the invention is to provide the application of the bactericidal composition in preventing and treating taro diseases.
Preferably, the taro diseases comprise taro epidemic disease, blight and taro stain disease.
Compared with the prior art, the invention has the following beneficial effects:
(1) The sterilization mechanisms of the epoxiconazole-oxammonium, the cyazofamid, the dimethomorph or the metalaxyl in the sterilization composition are different, so that the generation of drug resistance of pathogenic bacteria of the taro epidemic disease can be delayed, and the service life of the sterilization composition can be prolonged.
(2) When the 2 effective components in the bactericidal composition are compounded according to a certain mass ratio, the bactericidal composition has no cross resistance and shows better synergistic effect. Compared with a single active ingredient, the composition can improve the control effect on the phytophthora blight of the taro, is beneficial to reducing the application dosage of pesticide, reduces pesticide residue and control cost, and can provide support for developing novel medicaments.
Detailed Description
In the following, the technical solutions of the present invention will be described clearly and completely, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples: indoor activity test during compounding of fluxapyroxad
Reagent to be tested: 98% of fluxapyroxad (Xiongngda Nantong crop protection Co., ltd.), 94% of cyazofamid (Shanxi Meibang pharmaceutical industry group Co., ltd.), 98% of dimethomorph (Jiangsu Jia Longhua chemical Co., ltd.), and 95% of metalaxyl (Shandong He Yi Biotech Co., ltd.).
Test germs: a taro epidemic disease sample collected from a taro growing area in Guangxi Lipu city is separated in a laboratory, and the pathogenicity of the separated taro epidemic disease pathogen is determined by pathogenicity determination and morphological characteristic observation, so that the taro epidemic disease pathogen is Phytophthora canna (Phytophthora colocasiae) and is stored in a PDA culture medium. The PDA culture medium formula comprises: 200g of peeled potatoes, 20g of cane sugar, 20g of agar and 1000mL of water.
Test method (refer to NT/T1156.2-2006 agricultural chemical indoor bioassay test standard fungicide part 2: inhibition pathogenic fungus hypha growth test plate method)
1. Dissolving the test reagent with dimethyl sulfoxide, diluting with 0.1% Tween-80 water solution to obtain single mother liquid, and mixing the mother liquid and the mixed mother liquid in equal ratio to obtain 7 gradient mass concentrations.
2. Under aseptic conditions, adding 9mL of pre-melted PDA culture medium into an aseptic conical flask, quantitatively sucking 1.5mL of liquid medicine from low concentration to high concentration in sequence, adding into the conical flasks respectively, and shaking thoroughly. Then pouring the mixture into 3 culture dishes with the diameter of 9cm in equal amount to prepare drug-containing plates with corresponding concentrations; treatments without agent were blanked, 3 replicates of each treatment.
3. Under aseptic conditions, cutting bacterial cakes on the edges of the cultured pathogenic bacteria colonies of the phytophthora taro by using a puncher with the diameter of 5mm, inoculating the bacterial cakes in the centers of a drug-containing plate and a blank control plate, covering a dish cover, and then placing the plate at the constant temperature of 28 ℃ for culture and culture.
4. When the diameter of the pathogenic bacteria colony in the blank control culture dish reaches more than 1/2 of the diameter of the culture dish, measuring the diameter of the colony by using a caliper, and crossing each colony by using a crossMeasuring the diameter once by a method, taking the average value of the diameters, and calculating the hypha growth inhibition rate of each treatment; linear regression analysis is carried out by taking the logarithmic value of the concentration of the medicament as x and the corresponding value of the number of the hypha growth inhibition rate as y, and the EC of each treatment medicament is calculated 50 And calculating the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.
D＝D 1 -D 2
In the above formula: d- -colony growth diameter; d 1 -colony diameter; d 2 - -the diameter of the cake.
In the above formula: i- -hypha inhibition rate; d 0 -blank control colony growth diameter; d t Agent-treated colony growth diameter.
In the above formula: ATI- -the observed virulence index of the combination; s- -EC of Standard drug 50 In mg/L; m- -EC of test agent 50 The unit is mg/L.
TTI＝TI A ×P A +TI B ×P B
In the above formula: TTI- -theoretical toxicological index of the compound; TI A -the virulence index of the agent a; p is A -the percentage of agent a in the mixture in percent (%); TI B -virulence index of the B agent; p B The percentage of the agent B in the mixture is given in percentage (%).
In the above formula: CTC-co-toxicity coefficient; ATI- -measured virulence index of the mixture; TTI- -mixture theory virulence index.
The synergistic effect of the mixed medicament is evaluated according to the co-toxicity coefficient (CTC), namely the CTC is equal to or less than 80 and is antagonistic, the CTC is more than 80 and is less than 120 and is additive, and the CTC is equal to or more than 120 and is synergistic. The results are shown in tables 1-3.
TABLE 1 results of the determination of the indoor biological activity of the complex pair of fluxapyroxad and cyazofamid against phytophthora blight pathogenic bacteria
| Name and proportion of the medicament | EC 50 (mg/L) | ATI | TTI | CTC |
| Fluxapyroxad acyl hydroxylamine | 0.67 | 100.00 | -- | -- |
| Cyazofamid | 4.52 | 14.82 | -- | -- |
| Fluxapyroxad 1: cyazofamid 25 | 1.46 | 45.89 | 18.10 | 253.55 |
| Fluxapyroxad 1: cyazofamid 20 | 1.03 | 65.05 | 18.88 | 344.55 |
| Fluxapyroxad 1: cyazofamid 15 | 0.72 | 93.06 | 20.15 | 461.89 |
| Fluxapyroxad 1: cyazofamid 10 | 0.43 | 155.81 | 22.57 | 690.47 |
| Fluxapyroxad 1: cyazofamid 7 | 1.51 | 44.37 | 25.47 | 174.21 |
| Fluxapyroxad 1: cyazofamid 5 | 1.86 | 36.02 | 29.02 | 124.13 |
| Fluxapyroxad 1: cyazofamid 3 | 0.64 | 104.69 | 36.12 | 289.85 |
| Fluxapyroxad 1: cyazofamid 1 | 0.25 | 268.00 | 57.41 | 466.81 |
| Fluxapyroxad 2: cyazofamid 1 | 0.39 | 171.79 | 71.61 | 239.91 |
| Fluconazole hydroxylamine 3: cyazofamid 1 | 0.13 | 515.38 | 78.71 | 654.82 |
As can be seen from table 1, fluxapyroxad and cyazofamid are present in a mass ratio of 1 to 3: the cotoxicity coefficients within the range of 25-1 are all larger than 120, which shows that the biological activity of the composite of the fluxapyroxad and cyazofamid to the pathogenic bacteria of the taro blight shows synergistic effect, especially when the mass ratio is 1:10 and 3:1, the co-toxicity coefficient respectively reaches 690.47 and 654.82, and the synergistic effect is particularly remarkable.
TABLE 2 results of the indoor bioactivity determination of the composite pair of epoxiconazole hydroxylamine and dimethomorph against phytophthora cactorum
As can be seen from Table 2, the mass ratio of the fluxapyroxad to the dimethomorph is 1-10: the cotoxicity coefficients within the range of 5-1 are all larger than 120, which shows that the compounded epoxiconazole hydroxylamine and dimethomorph have synergistic effect on the biological activity of the phytophthora tarda pathogenic bacteria.
TABLE 3 results of the determination of the indoor biological activity of the pathogen of phytophthora blight by combining pyraflufen-ethyl and metalaxyl
| Name and proportion of the medicament | EC 50 (mg/L) | ATI | TTI | CTC |
| Fluxapyroxad acyl hydroxylamine | 0.67 | 100.00 | -- | -- |
| Metalaxyl | 6.17 | 10.86 | -- | -- |
| Fluxapyroxad 1: metalaxyl 40 | 3.29 | 20.36 | 13.03 | 156.25 |
| Fluxapyroxad 1: metalaxyl 30 | 2.11 | 31.75 | 13.73 | 231.20 |
| Fluxapyroxad 1: metalaxyl 20 | 0.57 | 117.54 | 15.10 | 778.24 |
| Fluxapyroxad 1: metalaxyl 10 | 0.62 | 108.06 | 18.96 | 569.88 |
| Fluxapyroxad 1: metalaxyl 7 | 1.33 | 50.38 | 22.00 | 228.96 |
| Fluxapyroxad 1: metalaxyl 5 | 2.13 | 31.46 | 25.72 | 122.32 |
| Fluxapyroxad 1: metalaxyl 3 | 0.89 | 75.28 | 33.14 | 227.13 |
| Fluconazole hydroxylamine 1: metalaxyl 1 | 0.28 | 239.29 | 55.43 | 431.69 |
| Fluxapyroxad 3: metalaxyl 1 | 0.51 | 131.37 | 77.71 | 169.04 |
| Fluxapyroxad 5: metalaxyl 1 | 0.18 | 372.22 | 85.14 | 437.17 |
| Fluxapyroxad 7: metalaxyl 1 | 0.43 | 155.81 | 88.86 | 175.35 |
| Fluxapyroxad 10: metalaxyl 1 | 0.26 | 257.69 | 91.90 | 280.42 |
| Fluxapyroxad 20: metalaxyl 1 | 0.35 | 191.43 | 95.76 | 199.91 |
| Fluxapyroxad 30: metalaxyl 1 | 0.49 | 136.73 | 97.12 | 140.78 |
As can be seen from Table 3, the mass ratio of the fluxapyroxad to the metalaxyl is 1-30: the cotoxicity coefficients within the range of 40-1 are all larger than 120, which shows that the compounded epoxiconazole hydroxylamine and dimethomorph have synergistic effect on the biological activity of the phytophthora tarda pathogenic bacteria.
In conclusion, the biological activity of the compound of the epoxiconazole oxamide, the cyazofamid, the dimethomorph or the metalaxyl on the phytophthora blight pathogenic bacteria shows a synergistic effect, and compared with a single effective component, the compound can effectively improve the control effect on the phytophthora blight.
Claims (1)
1. The application of the sterilization composition for preventing and treating the taro epidemic disease is characterized in that the effective component of the sterilization composition is formed by binary combination of fluxapyroxad, cyazofamid, dimethomorph or metalaxyl;
the mass ratio of the penflufen to the cyazofamid is (1-3): 25-1;
the mass ratio of the penflufen to the dimethomorph is 1-10:5-1;
the mass ratio of the fluxapyroxad to the metalaxyl is 1-30:40-1.
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