CN117281125A - Sterilization composition - Google Patents
Sterilization composition Download PDFInfo
- Publication number
- CN117281125A CN117281125A CN202311250629.7A CN202311250629A CN117281125A CN 117281125 A CN117281125 A CN 117281125A CN 202311250629 A CN202311250629 A CN 202311250629A CN 117281125 A CN117281125 A CN 117281125A
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- CN
- China
- Prior art keywords
- phenazine
- carboxylic acid
- imazalil
- mug
- bactericidal composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- NWWZPOKUUAIXIW-FLIBITNWSA-N thiamethoxam Chemical compound [O-][N+](=O)\N=C/1N(C)COCN\1CC1=CN=C(Cl)S1 NWWZPOKUUAIXIW-FLIBITNWSA-N 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 235000015398 thunder god vine Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a sterilization composition, which comprises the following components in percentage by mass of 0.5-5: 1 or the sterilization composition comprises imazalil and phenazine-1-carboxylic acid in a mass ratio of 1:3.4-5.5. The bactericidal composition has a synergistic effect on preventing and controlling rice sheath blight, strawberry gray mold or pepper anthracnose.
Description
Technical Field
The present invention relates to a plant bactericidal composition.
Background
Plants are the material basis upon which humans survive, with rice, strawberry, capsicum being important food and commercial crops. In recent years, with the improvement of the living standard of people, the agricultural products are diversified and have the qualityThe demand for growing rice, strawberry and capsicum is also increasing, and the planting area is also gradually expanding. These crops are often affected by a variety of factors during the growth process. For example: excessive fertilization can cause seedling firing at the root of crops, and leaves and stems are overgrown and easily lodged. Too little fertilization can cause the problems of small stems, insufficient seeds and the like. Thus, grasping the good fertilization period and fertilization amount is an important means for ensuring yield. Secondly, the degree of soil loosening can directly affect the activity of crop root systems and the ability to absorb nutrients. Again, soil pH affects plant height, tillering number and number of microorganisms in the crop. Also, different crop varieties are photosynthetic, ecologically adaptive, and CO-tolerant 2 There are large differences in the degree of response to the increase in concentration. In addition, cultivation measures, cultivation density and rotary cultivation depth can influence the yield of crops. Among all influencing factors, crop disease is one of the most important factors causing a decrease in its yield and quality. Rice sheath blight is caused by rhizoctonia, mainly infects leaf sheaths and leaves, and produces oval or moire lesions after being infested. When the humidity is high, white reticular hyphae grow on the affected part to form sclerotium, and the sclerotium is easy to fall off. The rice is infected to cause sheath blight, which causes increase of blighted grain, decrease of thousand grain weight, and even more serious, cause leaf blight and plant fall, generally the yield is reduced by 10% -30%, and the serious yield reaches more than 50%. The pepper anthracnose is mainly caused by fungus diseases of anthracnose, the diseases mainly damage leaves and fruits, the fruits are yellow brown, dense red dots and concentric ring patterns, the whole surface of the disease spots overflows red jelly in the later period, and when the disease is serious, the leaves can be fallen off, and the loss can reach more than 20%. The gray mold of the strawberries commonly occurs in the greenhouse and the open field cultivation, and the yield of the strawberries can be generally reduced by 20% -30%, more seriously, by more than 50%, so that huge economic loss is caused. The gray mold of strawberry is mainly caused by botrytis, which mainly damages the leaves and fruits, the typical symptoms of the leaf damage are V-shaped yellow brown spots until the scorch dies, the fruits are water-stained at the early stage of the disease attack of the mature fruits, and a thick gray mold layer is generated on the surface when the humidity is high. Based on the above, agricultural control is often used in productionBiological control and chemical control, wherein chemical control has the advantages of economy, high efficiency and convenience, is always the most main means, but long-term use of chemical pesticides is easy to cause drug resistance of bacteria, so that the drug effect is reduced. At present, validamycin is used for preventing and controlling rice sheath blight for more than 30 years, the drug effect starts to be reduced, pythium is used for preventing and controlling strawberry gray mold bacteria, reports on drug resistance are presented, difenoconazole is used for preventing and controlling pepper anthracnose bacteria, and drug resistance is discussed, so a novel method for exploring the drug resistance for preventing and controlling the crop diseases is unprecedented. In order to solve the problem of drug resistance of crop pathogens, various methods are available, such as: the bactericidal compound with novel structure is searched again, the times and the dosage of pesticides are reduced, the pesticides are used alternately, the pesticides are compounded, and the like, wherein the compounding of the pesticides with two different action mechanisms is the most effective and economical method, the service life of the pesticide can be prolonged, and the control effect is improved.
Chinese patent publication No. CN1961678A uses powder composed of chlorine dioxide, moroxydine hydrochloride, cytokinin and microbial products to control rice sheath blight. The Chinese patent with publication number of CN109197890A adopts a composite seed coating agent consisting of thiamethoxam 0.9-1.8%, curculin 0.3-1.2%, tetramycin 0.05-0.3%, forchlorfenuron 0.2-0.5%, film forming agent 6-10%, dispersing agent 4-8%, thickening agent 0.1-1%, alkaline rose extract 0.3-0.5%, salicylic acid 0.01-0.1%, trace elements 0.01-0.1% and the balance of water to prevent and treat rice sheath blight. The Chinese patent with publication number CN107624795A adopts fermentation extract and validamycin to prevent and treat rice sheath blight disease. The Chinese patent with publication number of CN104106579B adopts the compound bactericide of the albendazole and the azoxystrobin with the mass ratio of 5:1-1:3 to prevent and treat rice sheath blight. The Chinese patent with the publication number of CN104381292A adopts 2-4 parts of acetamiprid, 2-4 parts of kungfu, 4-5 parts of gibberellin, 7-10 parts of zinc sulfate, 7-10 parts of ammonium molybdate, 10-12 parts of abamectin, 12-14 parts of chlorotoxicide, 30-35 parts of humic acid organic liquid fertilizer, 2-4 parts of methyl sterol, 1-2 parts of pyrimidyl salicylic acid, 4-5 parts of oleic acid, 7-10 parts of primary distillate oil, 8-22 parts of light oil and 4-6 parts of pesticide emulsifier to prevent and treat rice sheath blight.
The Chinese patent with publication number CN1961678A adopts bactericide composed of 5-9 parts of astragalus root, 5-9 parts of coptis root, 5-9 parts of radix euphorbiae Fischerianae, 4-6 parts of ginkgo, 3-5 parts of radix sophorae flavescentis, 1-3 parts of herba artemisiae capillaries, 1-3 parts of eupatorium, 2-4 parts of folium artemisiae argyi, 2-4 parts of gallnut, 5-7 parts of honeysuckle, 5-7 parts of peppermint and 6-10 parts of garlic to prevent and treat pepper anthracnose. The Chinese patent with publication number CN113907083B adopts bactericide prepared from tetramycin and prochloraz as effective components to prevent and treat pepper anthracnose. Chinese patent publication No. CN107156183A is a bactericide prepared from Piper sarmentosum extract and benthiavalicarb-isopropyl for preventing and treating pepper anthracnose. Chinese patent publication No. CN107156183A is a bactericide prepared from Piper sarmentosum extract and benthiavalicarb-isopropyl for preventing and treating pepper anthracnose.
Chinese patent publication No. CN105028503A adopts mugwort leaf, purple perilla, rhizoma atractylodis, pumpkin seed, kuh-seng, cyrtomium rhizome, golden larch bark, ephedra, hemp leaf nettle, cassia twig and pomegranate rind to prevent and treat gray mold of strawberry. The Chinese patent with publication number of CN106106587A adopts 25-30 parts of Rumex madaio, 15-20 parts of notoginseng leaf, 13-17 parts of tobacco straw, 10-15 parts of subprostrate sophora, 10-15 parts of Chinese alangium root, 8-13 parts of tripterygium wilfordii, 6-10 parts of carpesium, 5-8 parts of dried ginger, 5-8 parts of fructus gleditsiae, 4-7 parts of tea oil cake, 3-6 parts of ore powder, 3-6 parts of semen allii tuberosi, 2-4 parts of castor bean, 0.5-1 part of chromium trichloride and 0.5-1 part of nano zinc oxide to prevent and treat gray mold of strawberry. Chinese patent publication CN103651373a uses cyprodinil and pyrimethanil to control gray mold of strawberry.
The overall effect of the above patents is not ideal, so that development of a bactericidal composition with good control effect on rice sheath blight disease, pepper anthracnose or strawberry gray mold is needed.
The CN108041057B patent discloses a compound bactericide which has good environmental compatibility, high efficiency, low toxicity and low residue, is composed of Zhongshengmycin and imazalil, is especially used for the application research of preventing and curing main diseases of fruit trees such as apple ring spot, apple anthracnose, grape white rot, grape anthracnose and the like, the weight ratio of the two active ingredients of Zhongshengmycin and imazalil in the compound composition is 60:1-1:60, the synergistic ratio is 4:1-1:20, and the optimal ratio is 1:4. The invention has outstanding performance in the aspect of preventing and controlling main diseases of fruit trees such as apple ring spot, apple anthracnose, grape white rot, grape anthracnose and the like. The patent is only applicable to diseases of fruit trees, and the invention is also applicable to diseases of gramineous crops.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a sterilization composition which has a synergistic effect in preventing and controlling rice sheath blight, strawberry gray mold or pepper anthracnose.
In order to solve the technical problems, the invention adopts the following technical scheme: the bactericidal composition comprises the following components in percentage by mass of 0.5-5: 1 or the sterilization composition comprises imazalil and phenazine-1-carboxylic acid in a mass ratio of 1:3.4-5.5.
Imazalil is an imidazole compound, belongs to a moderate-toxicity bactericide, is commonly used for fresh-keeping during fruit storage, and has high control effect on plant pathogenic fungi such as long-child spore, fusarium, aschersonia and the like. The existing imazalil bacteriostasis mechanism may influence the permeability, physiological functions and lipid anabolism of fungal cell membranes, so that the fungal cell membranes are destroyed, the fungal cell membranes cannot be normally proliferated, and the normal life of the fungal cell membranes is influenced. Through repeated screening tests, the imazalil and various bacteriostat combinations have no synergistic effect.
The phenazine-1-carboxylic acid is a carboxyl substituted phenazine compound, and is a novel fungicidal compound which is efficient, low in toxicity and good in environmental compatibility. Currently, the possible mechanism of action for phenazine-1-carboxylic acids is to disrupt the structure of the cell wall and cytoplasmic membrane and physiological activity to exert fungicidal action. Through repeated screening tests, the phenazine-1-carboxylic acid and the combination of various bacteriostats have no synergistic effect.
According to the invention, two chemical agents are compounded, and the unexpected discovery that imazalil and phenazine-1-carboxylic acid with certain mass ratio can expand the antibacterial range, slow drug resistance generation, prolong the service time and improve the control effect.
The mass ratio of imazalil to phenazine-1-carboxylic acid is 0.5-5: 1 or 1:3.4-5.5, has a synergistic effect in controlling rice sheath blight, strawberry gray mold or pepper anthracnose.
In a preferred embodiment of the present invention, the sterilizing composition comprises 1 to 4 mass ratio: 1 or the bactericidal composition comprises imazalil and phenazine-1-carboxylic acid according to the mass ratio of 1:3.5 to 5.5 of imazalil and phenazine-1-carboxylic acid, preferably the bactericidal composition comprises 2 to 4 of the following components in mass ratio: 1 and phenazine-1-carboxylic acid.
In a preferred embodiment of the present invention, the EC of the fungicidal composition 50 0.08-0.6 mug/mL;
the pH value of the environment of the sterilizing composition in the use process is less than or equal to 7.
In a preferred embodiment of the present invention, the total mass concentration of the bactericidal composition is 0.2 to 0.5 μg/mL, preferably the total mass concentration of the bactericidal composition is 0.3 to 0.4 μg/mL.
In a preferred embodiment of the present invention, the sterilizing composition comprises 4 to 5 mass ratio: 1 and phenazine-1-carboxylic acid; or the bactericidal composition comprises imazalil and phenazine-1-carboxylic acid in a mass ratio of 1:3.4-4.5.
In a preferred embodiment of the present invention, the EC of the fungicidal composition 50 0.08-4.1 mug/mL;
the pH value of the environment of the sterilizing composition in the use process is less than or equal to 7.
In a preferred embodiment of the present invention, the total mass concentration of the bactericidal composition is 3.6 to 4.1 μg/mL, preferably the total mass concentration of the bactericidal composition is 3.8 to 4.0 μg/mL.
In a preferred embodiment of the present invention, the sterilizing composition comprises 0.5 to 3 by mass: 1 and phenazine-1-carboxylic acid, preferably, the bactericidal composition comprises the components with the mass ratio of 0.5-1.5: 1 and phenazine-1-carboxylic acid.
In a preferred embodiment of the present invention, the EC of the fungicidal composition 50 2.4-2.8 mug/mL;
the pH value of the environment of the sterilizing composition in the use process is less than or equal to 7.
In a preferred embodiment of the present invention, the total mass concentration of the bactericidal composition is 8 to 12 μg/mL, preferably the total mass concentration of the bactericidal composition is 9 to 10 μg/mL.
The invention also discloses an application of the bactericidal composition in preventing and controlling rice sheath blight, and the bactericidal composition comprises the following components in percentage by mass: 1 or the bactericidal composition comprises imazalil and phenazine-1-carboxylic acid according to the mass ratio of 1:3.5 to 5.5 of imazalil and phenazine-1-carboxylic acid, preferably the bactericidal composition comprises 2 to 4 of the following components in mass ratio: 1 and phenazine-1-carboxylic acid.
The total mass concentration of the bactericidal composition refers to the sum of the mass concentrations of all the components in the bactericidal composition, such as the sum of the mass concentrations of imazalil and phenazine-1-carboxylic acid.
In a preferred embodiment of the present invention, the EC of the fungicidal composition 50 0.08-0.6 mug/mL;
the pH value of the environment in the use process of the bactericidal composition is less than or equal to 7, the bactericidal composition is not suitable for alkaline environments, otherwise, the control effect is reduced, the bactericidal composition is suitable for acidic environments, and the control effect can be effectively ensured. Wherein the environment comprises soil.
In a preferred embodiment of the present invention, the total mass concentration of the bactericidal composition is 0.2 to 0.5 μg/mL, preferably the total mass concentration of the bactericidal composition is 0.3 to 0.4 μg/mL.
The invention also discloses an application of the sterilizing composition in preventing and treating the gray mold of the strawberries, wherein the sterilizing composition comprises the following components in percentage by mass: 1 and phenazine-1-carboxylic acid; or the bactericidal composition comprises imazalil and phenazine-1-carboxylic acid in a mass ratio of 1:3.4-4.5.
In a preferred embodiment of the present invention, the EC of the fungicidal composition 50 0.08-4.1 mug/mL;
the pH value of the environment of the sterilizing composition in the use process is less than or equal to 7.
In a preferred embodiment of the present invention, the total mass concentration of the bactericidal composition is 3.6 to 4.1 μg/mL, preferably the total mass concentration of the bactericidal composition is 3.8 to 4.0 μg/mL.
The invention also discloses an application of the bactericidal composition in preventing and controlling pepper anthracnose, wherein the bactericidal composition comprises the following components in percentage by mass of 0.5-3: 1 and phenazine-1-carboxylic acid, preferably, the bactericidal composition comprises the components with the mass ratio of 0.5-1.5: 1 and phenazine-1-carboxylic acid.
In a preferred embodiment of the present invention, the EC of the fungicidal composition 50 2.4-2.8 mug/mL;
the pH value of the environment of the sterilizing composition in the use process is less than or equal to 7.
In a preferred embodiment of the present invention, the total mass concentration of the bactericidal composition is 8 to 12 μg/mL, preferably the total mass concentration of the bactericidal composition is 9 to 10 μg/mL.
The imazalil and phenazine-1-carboxylic acid are compounded according to five proportions of 4:1, 3:1, 2:1, 1:1 and 1:5 to have synergistic effects on Rhizoctonia solani, wherein the synergistic coefficient of 4:1 is highest and reaches 4.4, and the synergistic coefficient is 2:1, 1:1, 3:1 and the lowest synergistic coefficient is 1:5. The imazalil and phenazine-1-carboxylic acid are compounded in a ratio of 5:1, 4:1 and 1:4 to have synergistic effects on the botrytis cinerea, wherein the highest synergistic coefficient of 4:1 is 2.26, the second is 5:1, and the last is 1:4. The synergistic effect of the imazalil and the phenazine-1-carboxylic acid is achieved by compounding the imazalil and the phenazine-1-carboxylic acid in a ratio of 1:1, 2:1 and 3:1, wherein the synergistic coefficient is 1.99 when the mass ratio of the imazalil to the phenazine-1-carboxylic acid is 1:1. The dosage of the patent is less than that of the prior art, and the synergy is good.
Compared with the prior art, the invention has the following beneficial effects:
1. the synergistic effect of imazalil and phenazine-1-carboxylic acid on Rhizoctonia solani, botrytis cinerea and pepper anthracnose is found for the first time, which provides a theoretical basis for the subsequent preparation of new pesticides.
2. The preparation is compounded by two medicaments with different action mechanisms, namely imazalil and phenazine-1-carboxylic acid, so that the service life of the medicament can be prolonged, and the pathogen is not easy to generate drug resistance. 3. The invention provides a compound for inhibiting multiple plant pathogenic fungi, which enriches candidate bactericides for inhibiting multiple plant pathogenic fungi.
Drawings
FIG. 1 shows the synergistic effect of imazalil and phenazine-1-carboxylic acid (4:1) on Rhizoctonia solani in an embodiment of the invention.
Note that: a is CK; b, 10 mug/mL; c2 mug/mL; d, 0.4 mug/mL; e0.08 mug/mL; f, 0.016. Mu.g/mL.
FIG. 2 shows the synergistic effect of imazalil and phenazine-1-carboxylic acid (2:1) on Rhizoctonia solani in example 1 of the invention.
Note that: a is CK; b, 10 mug/mL; c2 mug/mL; d, 0.4 mug/mL; e0.08 mug/mL; f, 0.016. Mu.g/mL.
FIG. 3 shows the synergistic effect of imazalil and phenazine-1-carboxylic acid (1:1) on Rhizoctonia solani in example 1 of the invention.
Note that: a is CK; b, 10 mug/mL; c2 mug/mL; d, 0.4 mug/mL; e0.08 mug/mL; f, 0.016. Mu.g/mL.
FIG. 4 shows the synergistic effect of imazalil and phenazine-1-carboxylic acid (3:1) on Rhizoctonia solani in example 1 of the invention.
Note that: a is CK; b, 10 mug/mL; c2 mug/mL; d, 0.4 mug/mL; e0.08 mug/mL; f, 0.016. Mu.g/mL.
FIG. 5 shows the synergistic effect of imazalil and phenazine-1-carboxylic acid (4:1) on Botrytis cinerea in example 2 of the present invention.
Note that: a is CK; b, 10 mug/mL; c2 mug/mL; d, 0.4 mug/mL; e0.08 mug/mL; f, 0.016. Mu.g/mL.
FIG. 6 shows the synergistic effect of imazalil and phenazine-1-carboxylic acid (5:1) on Botrytis cinerea in example 2 of the present invention: a is CK; b, 10 mug/mL; c2 mug/mL; d, 0.4 mug/mL; e0.08 mug/mL; f, 0.016. Mu.g/mL.
FIG. 7 shows the synergistic effect of imazalil and phenazine-1-carboxylic acid (1:4) on Botrytis cinerea in example 2 of the present invention: a is CK; b, 10 mug/mL; c2 mug/mL; d, 0.4 mug/mL; e0.08 mug/mL; f, 0.016. Mu.g/mL.
FIG. 8 shows the synergistic effect of imazalil and phenazine-1-carboxylic acid (1:1) on pepper anthracnose in example 3 of the present invention.
Note that: a is CK; b, 18.18 mug/mL; c9.09 μg/mL; d4.55. Mu.g/mL; e2.27. Mu.g/mL; f1.13. Mu.g/mL.
FIG. 9 shows the synergistic effect of imazalil and phenazine-1-carboxylic acid (2:1) on pepper anthracnose in example 3 of the present invention.
Note that: a is CK; 14.29 mug/mL; c7.15 mug/mL; d3.58 μg/mL; e1.79 mug/mL; f, 0.90. Mu.g/mL.
FIG. 10 shows the synergistic effect of imazalil and phenazine-1-carboxylic acid (3:1) on pepper anthracnose in example 3 of the present invention.
Note that: a is CK; b, 12.90 mug/mL; c, 6.45 mug/mL; d3.28. Mu.g/mL; e1.64. Mu.g/mL; f, 0.82. Mu.g/mL.
Detailed Description
The following describes the technical scheme of the invention with reference to specific tests and examples, wherein the percentages related to the tests and examples are mass percentages.
The raw materials used in the invention are supplied sufficiently, and the obtained paths are various.
Example 1:
(1) Test material
Test pathogens used in this test: rhizoctonia solani (Rhizoctonia solani) is provided by the plant pest biology and control major laboratory of the university of agriculture plant protection institute of Hunan province.
The agent used in this experiment: 97% imazalil was purchased from national pharmaceutical group chemical company, inc; 92% of phenazine-1-carboxylic acid was developed by the agricultural microbiology laboratory of Hunan university; other analytical reagents, absolute ethanol, tween-80, acetone, glucose, agar strips, etc., were purchased from the biological sciences limited of wutai in the hunan.
Test medium: PDA culture medium, 200g potato, 20g glucose, 20g agar, pH value is natural, water is added to fix volume to 1L, and sterilization is carried out for 20min at 121 ℃ under 1 atmosphere for standby.
(2) Determination of imazalil and phenazine-1-carboxylic acid virulence
The inhibition of rice sheath blight by imazalil and phenazine-1-carboxylic acid was determined by hypha growth rate method. Firstly, weighing a proper amount of imazalil and phenazine-1-carboxylic acid on an analytical balance by using weighing filter paper, pouring the weighed crude drug into a dry conical flask, and adding a proper amount of acetone @Not more than 0.2% of the volume of the mother solution), dissolving imazalil and phenazine-1-carboxylic acid, adding a proper amount of sterile water to prepare 1000 mug/mL mother solution (if the water solubility of the raw materials is poor, a proper amount of Tween-80 can be added), and finally placing the mother solution into an ultrasonic cleaner for ultrasonic treatment for 25min or placing the mother solution into a water bath kettle at a proper temperature for water bath for 30min. Sterile water was added and the stock solution was diluted to 500. Mu.g/mL, 250. Mu.g/mL, 125. Mu.g/mL, 62.5. Mu.g/mL. 9mL of PDA culture medium melted to 50-60 ℃ is taken by a liquid-transferring gun, placed into a culture dish with the diameter of 90mm, and then 1mL of liquid medicine is added to be uniformly mixed, so as to prepare the medicine-containing culture medium. At the same time, an equal volume of sterile water was added to the control, and 3 replicates were set for each treatment. A hole puncher with the diameter of 6mm is used for punching holes on the outermost edge of a culture dish with the mycelium growing approximately 2/3, and a fungus cake with the mycelium is placed on the solidified culture dish by an inoculating loop. Culturing in a constant temperature incubator at 24deg.C in the absence of light, measuring the growth diameter of mycelium by crisscross measurement when mycelium grows to 2/3 of the culture dish, and taking average value. The concentrations of the two compounds of Rhizoctonia solani were determined according to the inhibition rates of 100. Mu.g/mL, 50. Mu.g/mL, 25. Mu.g/mL, 12.5. Mu.g/mL and 6.25. Mu.g/mL for the plates with toxicity, and are shown in Table 1. The inhibition ratio of imazalil and phenazine-1-carboxylic acid to Rhizoctonia solani is calculated according to the following formula, and DPS9.01 is used for processing data to obtain a correlation coefficient and an inhibition medium concentration (EC) 50 ) And regression equations.
Wherein, the acetone has strong solubility and good inertia. Tween-80 mainly plays a role in emulsification.
(3) Synergistic effect of imazalil and phenazine-1-carboxylic acid on Rhizoctonia solani
Determining toxicity of imazalil and phenazine-1-carboxylic acid mixture to Rhizoctonia solani by hypha growth rate method, compounding imazalil and phenazine-1-carboxylic acid according to the proportion given in Table 2, adding the compounded mixed medicament into sterilized culture dish, pouring into PDA culture medium at 50-60deg.C, adding equal volume of non-aqueous medium into controlBacterial water was designed 3 replicates per treatment, holes were punched with a 6mm diameter punch on the outermost edge of the petri dish where hyphae grew approximately 2/3, and a one-sided bacterial cake with hyphae was placed with an inoculating loop on the coagulated petri dish. Culturing in a constant temperature incubator at 24deg.C in the absence of light, measuring the growth diameter of mycelium by crisscross measurement when mycelium grows to 2/3 of the culture dish, and taking average value. According to the calculation method in the formula (1), the inhibition rate of the compound agent is obtained, DPS9.01 is used for processing data, and EC is obtained 50 And regression equations. And calculating the synergy coefficient of each compound medicament according to the wadley method to evaluate whether the compound medicament has synergy.
EC 50 (th)＝(a+b)/[a/EC(A) 50 +b/EC(B) 50 ] (2)
SR＝EC 50 (th)/EC 50 (ob) (3)
In the formula, A and B respectively represent two compound medicaments, a and B respectively represent the proportion of the two medicaments in the mixture, ob is an actual observed value, and th is a theoretical value. SR >1.5 is synergistic; SR is more than or equal to 0.5 and less than or equal to 1.5 and takes addition effect; SR <0.5 is antagonism.
TABLE 1 concentration design of imazalil and phenazine-1-carboxylic acid to inhibit Rhizoctonia solani
Wherein the first row is double dilution method concentration and the second row is five times dilution method concentration.
TABLE 2 design of different specific concentration of imazalil and phenazine-1-carboxylic acid complexes for inhibiting Rhizoctonia solani
(4) Results and analysis
4.1 determination of toxicity of imazalil and phenazine-1-Carboxylic acid to Rhizoctonia solani
As is clear from Table 3, the combination of imazalil and phenazine-1-carboxylic acid against Rhizoctonia solaniAll have inhibiting effect. The imazalil has the strongest toxicity to Rhizoctonia solani, and the EC thereof 50 0.3818. Mu.g/mL, the virulence of phenazine-1-carboxylic acid is inferior, the EC thereof 50 0.8561. Mu.g/mL, 0.4460 times that of imazalil.
TABLE 3 toxicity of imazalil and phenazine-1-carboxylic acid single dose and compounded against Rhizoctonia solani
4.2 synergistic effects of imazalil and phenazine-1-carboxylic acid on Rhizoctonia solani
As shown in Table 3, the synergistic coefficient was the lowest when imazalil was compounded with phenazine-1-carboxylic acid in a ratio of 1:2, and was 0.65. The maximum efficiency of the formulation at a 4:1 ratio is approximately 7 times the 1:2 efficiency, reaching 4.41 (FIG. 1). When compounded in a 2:1 ratio, the synergy is 4.1, slightly below the maximum synergy (figure 2). 1, the method comprises the following steps: when compounded in a ratio of 1, the synergy is 3.14, which is about 5 times the lowest synergy (fig. 3). When compounded in a 3:1 ratio, the synergy coefficient is 2.35, and the effect is general (figure 4).
Example 2:
(1) Test material
Test pathogens used in this test: botrytis cinerea is provided by the plant pest biology and control major laboratory of Hunan university of agriculture, proc. Plant diseases and insect pests, hunan province.
The agent used in this experiment: 97% imazalil was purchased from national pharmaceutical group chemical company, inc; 92% of phenazine-1-carboxylic acid was developed by the agricultural microbiology laboratory of Hunan university; other analytical reagents, absolute ethanol, tween-80, acetone, glucose, agar strips, etc., were purchased from the biological sciences limited of wutai in the hunan.
Test medium: PDA culture medium, 200g potato, 20g glucose, 20g agar, pH value is natural, water is added to fix volume to 1L, and sterilization is carried out for 20min at 121 ℃ under 1 atmosphere for standby.
(2) Determination of imazalil and phenazine-1-carboxylic acid virulence
The inhibition of the fungus Gray strawberry by imazalil and phenazine-1-carboxylic acid was determined by a hypha growth rate method. Firstly, weighing a proper amount of imazalil and phenazine-1-carboxylic acid on an analytical balance by using weighing filter paper, pouring the weighed crude drugs into a dry conical flask, adding a proper amount of acetone (not more than 0.2% of the volume of mother liquor) to dissolve the two crude drugs of imazalil and phenazine-1-carboxylic acid, adding a proper amount of sterile water to prepare 1000 mug/mL of mother liquor (if the crude drugs are poor in water solubility, a proper amount of Tween-80 can be added), and finally, placing the mother liquor into an ultrasonic cleaner for ultrasonic treatment for 25min or placing the mother liquor into a water bath kettle at a proper temperature for 30min. Sterile water was added and the stock solution was diluted to 500. Mu.g/mL, 250. Mu.g/mL, 125. Mu.g/mL, 62.5. Mu.g/mL. 9mL of PDA culture medium melted to 50-60 ℃ is taken by a liquid-transferring gun, placed into a culture dish with the diameter of 90mm, and then 1mL of liquid medicine is added to be uniformly mixed, so as to prepare the medicine-containing culture medium. At the same time, an equal volume of sterile water was added to the control, and 3 replicates were set for each treatment. A hole puncher with the diameter of 6mm is used for punching holes on the outermost edge of a culture dish with the mycelium growing approximately 2/3, and a fungus cake with the mycelium is placed on the solidified culture dish by an inoculating loop. Culturing in a constant temperature incubator at 24deg.C in the absence of light, measuring the growth diameter of mycelium by crisscross measurement when mycelium grows to 2/3 of the culture dish, and taking average value. The concentrations of the two compounds of the Botrytis cinerea are determined according to the inhibition rates of 100 mug/mL, 50 mug/mL, 25 mug/mL, 12.5 mug/mL and 6.25 mug/mL of the flat plate with toxin as shown in the table 4. The inhibition ratio of imazalil and phenazine-1-carboxylic acid to Botrytis cinerea is calculated according to the following formula, and DPS9.01 is used for processing data to obtain a correlation coefficient and an inhibition medium concentration (EC 50 ) And regression equations.
(3) Synergistic effect of imazalil and phenazine-1-carboxylic acid on Botrytis cinerea
The toxicity of the imazalil and phenazine-1-carboxylic acid mixture to the Botrytis cinerea is measured by adopting a mycelium growth rate method, the imazalil and the phenazine-1-carboxylic acid are compounded according to the proportion given in the table 5, the compounded mixed medicament is added into a sterilized culture dish, a PDA culture medium with the temperature of 50-60 ℃ is poured into the culture dish, an equal volume of sterile water is added into a control, each treatment is designed to be 3 times, a puncher with the diameter of 6mm is used for punching on the outermost edge of the culture dish with the mycelium growing close to 2/3, and a one-sided fungus cake with the mycelium is placed on the solidified culture dish by using an inoculating loop. Culturing in a constant temperature incubator at 24deg.C in the absence of light, measuring the growth diameter of mycelium by crisscross measurement when mycelium grows to 2/3 of the culture dish, and taking average value. According to the calculation method in (1), the inhibition rate of the compound agent is obtained, DPS9.01 is used for processing data to obtain EC 50 And regression equations. And calculating the synergy coefficient of each compound medicament according to the Wadley method to evaluate whether the compound medicament has synergy.
EC 50 (th)＝(a+b)/[a/EC(A) 50 +b/EC(B) 50 ] (2)
SR＝EC 50 (th)/EC 50 (ob) (3)
In the formula, A and B respectively represent two compound medicaments, a and B respectively represent the proportion of the two medicaments in the mixture, ob is an actual observed value, and th is a theoretical value. SR >1.5 is synergistic; SR is more than or equal to 0.5 and less than or equal to 1.5 and takes addition effect; SR <0.5 is antagonism.
TABLE 4 concentration design of imazalil and phenazine-1-carboxylic acid to inhibit Gray mildew of strawberry
TABLE 5 different ratio concentration designs of imazalil and phenazine-1-carboxylic acid complexes for inhibiting Botrytis cinerea
(4) Results and analysis
4.1 determination of toxicity of imazalil and phenazine-1-Carboxylic acid to Botrytis cinerea
As shown in Table 6, imazalil and phenazine-1-carboxylic acid both inhibited growth, wherein imazalil had the strongest virulence against Botrytis cinerea, and EC thereof 50 1.80. Mu.g/mL, and EC of phenazine-1-carboxylic acid 50 17.0783. Mu.g/mL, indicating that imazalil is more toxic than phenazine-1-carboxylic acid.
TABLE 6 toxicity of imazalil and phenazine-1-carboxylic acid Single dose and Compound against Botrytis cinerea
4.2 synergistic effects of imazalil and phenazine-1-carboxylic acid on Botrytis cinerea
As shown in Table 6, the combination of imazalil and phenazine-1-carboxylic acid in the ratio of 4:1, 5:1 and 1:4 has higher synergism, wherein the ratio of 4: the maximum synergy coefficient of the 1 is 2.26 (figure 5). And the second is 5:1, the synergy coefficient reaches 1.91 (figure 6). The smallest synergistic coefficient of the three is compounded in a ratio of 1:4 (figure 7). In the adopted compounding proportion, the lowest synergy coefficient is 0.28 when compounding is carried out in a proportion of 1:5.
Example 3:
(1) Test material
Test pathogens used in this test: the pepper anthracnose germ (Colletotrichum capsici) is provided by the plant disease and pest biology and control major laboratory of the university of agriculture plant protection institute of Hunan province.
The agent used in this experiment: 97% imazalil was purchased from national pharmaceutical group chemical company, inc; 92% of phenazine-1-carboxylic acid was developed by the agricultural microbiology laboratory of Hunan university; other analytical reagents, absolute ethanol, tween-80, acetone, glucose, agar strips, etc., were purchased from the biological sciences limited of wutai in the hunan.
Test medium: PDA culture medium, 200g potato, 20g glucose, 20g agar, pH value is natural, water is added to fix volume to 1L, and sterilization is carried out for 20min at 121 ℃ under 1 atmosphere for standby.
(2) Determination of imazalil and phenazine-1-carboxylic acid virulence
The inhibition of the bacteria anthracnose of capsicum by imazalil and phenazine-1-carboxylic acid is determined by a hypha growth rate method. Firstly, weighing a proper amount of imazalil and phenazine-1-carboxylic acid on an analytical balance by using weighing filter paper, pouring the weighed crude drugs into a dry conical flask, adding a proper amount of acetone (not more than 0.2% of the volume of mother liquor) to dissolve the two crude drugs of imazalil and phenazine-1-carboxylic acid, adding a proper amount of sterile water to prepare 1000 mug/mL of mother liquor (if the crude drugs are poor in water solubility, a proper amount of Tween-80 can be added), and finally, placing the mother liquor into an ultrasonic cleaner for ultrasonic treatment for 25min or placing the mother liquor into a water bath kettle at a proper temperature for 30min. Sterile water was added and the stock solution was diluted to 500. Mu.g/mL, 250. Mu.g/mL, 125. Mu.g/mL, 62.5. Mu.g/mL. 9mL of PDA culture medium melted to 50-60 ℃ is taken by a liquid-transferring gun, placed into a culture dish with the diameter of 90mm, and then 1mL of liquid medicine is added to be uniformly mixed, so as to prepare the medicine-containing culture medium. At the same time, an equal volume of sterile water was added to the control, and 3 replicates were set for each treatment. A hole puncher with the diameter of 6mm is used for punching holes on the outermost edge of a culture dish with the mycelium growing approximately 2/3, and a fungus cake with the mycelium is placed on the solidified culture dish by an inoculating loop. Culturing in a constant temperature incubator at 24deg.C in the absence of light, measuring the growth diameter of mycelium by crisscross measurement when mycelium grows to 2/3 of the culture dish, and taking average value. The concentrations of the two compounds compounded for the pepper anthracnose bacteria were determined according to the inhibition rates of 100 mug/mL, 50 mug/mL, 25 mug/mL, 12.5 mug/mL and 6.25 mug/mL of the flat plate with toxin are shown in Table 7. The inhibition ratio of imazalil and phenazine-1-carboxylic acid to pepper anthracnose pathogen is calculated according to the following formula, DPS9.01 is used for processing the data to obtain the correlation coefficient and the inhibition medium concentration (EC 50 ) And regression equations.
(3) Synergistic effect of imazalil and phenazine-1-carboxylic acid on pepper anthracnose pathogen
The toxicity of imazalil and phenazine-1-carboxylic acid mixture to pepper anthracnose bacteria is determined by adopting a mycelium growth rate method, the imazalil and the phenazine-1-carboxylic acid are compounded according to the proportion given in table 8, the compounded mixture is added into a sterilized culture dish, a PDA culture medium with the temperature of 50-60 ℃ is poured into the culture dish, an equal volume of sterile water is added into a control, 3 repetitions of each treatment are designed, a puncher with the diameter of 6mm is used for punching on the outermost edge of the culture dish with mycelium growth close to 2/3, and a one-sided fungus cake with mycelium is placed on the solidified culture dish by using an inoculating loop. Culturing in a constant temperature incubator at 24deg.C in the absence of light, measuring the growth diameter of mycelium by crisscross measurement when mycelium grows to 2/3 of the culture dish, and taking average value. According to the calculation method in (1), the inhibition rate of the compound agent is obtained, DPS9.01 is used for processing data to obtain EC 50 And regression equations. And calculating the synergy coefficient of each compound medicament according to the Wadley method to evaluate whether the compound medicament has synergy.
EC 50 (th)＝(a+b)/[a/EC(A) 50 +b/EC(B) 50 ] (2)
SR＝EC 50 (th)/EC 50 (ob) (3)
In the formula, A and B respectively represent two compound medicaments, a and B respectively represent the proportion of the two medicaments in the mixture, ob is an actual observed value, and th is a theoretical value. SR >1.5 is synergistic; SR is more than or equal to 0.5 and less than or equal to 1.5 and takes addition effect; SR <0.5 is antagonism.
TABLE 7 concentration design of imazalil and phenazine-1-carboxylic acid to inhibit Capsici fructus anthracnose
Table 8 different ratio concentration designs of imazalil and phenazine-1-carboxylic acid compound for inhibiting pepper anthracnose pathogen
(4) Results and analysis
4.1 determination of toxicity of imazalil and phenazine-1-Carboxylic acid to Capsicum anthracnose
As shown in Table 9, imazalil and phenazine-1-carboxylic acid can inhibit the growth of pepper anthracnose, and imazalil has the strongest toxicity to pepper anthracnose, and its EC 50 2.7375. Mu.g/mL, EC of phenazine-1-carboxylic acid 50 EC of phenazine-1-carboxylic acid at 24.94. Mu.g/mL 50 About 9 times that of imazalil. The toxicity of imazalil to phytophthora capsici is higher than that of phenazine-1-carboxylic acid.
TABLE 9 toxicity of imazalil and phenazine-1-carboxylic acid single dose and compounded against pepper anthracnose pathogen
4.2 synergistic effects of imazalil and phenazine-1-carboxylic acid on pepper anthracnose pathogen
As shown in Table 9, the synergistic coefficient was relatively large at the ratios of 1:1, 2:1, and 3:1, 1.99,1.43 and 1.41, respectively (FIGS. 8-10). The minimum synergy coefficient is 0.22 when the composition is compounded in a ratio of 5:1, which is far lower than the synergy coefficient when the composition is compounded in a ratio of 1:1.
Example 4:
(1) Test material
Test pathogens used in this test: rhizoctonia solani (Rhizoctonia solani) is provided by the plant pest biology and control major laboratory of the university of agriculture plant protection institute of Hunan province.
The agent used in this experiment: 97% imazalil was purchased from national pharmaceutical group chemical company, inc; 92% of phenazine-1-carboxylic acid was developed by the agricultural microbiology laboratory of Hunan university; other analytical reagents, absolute ethanol, tween-80, acetone, glucose, agar strips, etc., were purchased from the biological sciences limited of wutai in the hunan.
Test medium: PDA culture medium, 200g potato, 20g glucose, 20g agar, pH value is natural, water is added to fix volume to 1L, and sterilization is carried out for 20min at 121 ℃ under 1 atmosphere for standby.
(2) In-vitro leaf control effect of imazalil and phenazine-1-carboxylic acid on rice sheath blight disease
The imazalil and phenazine-1-carboxylic acid compound is formed into a liquid medicine with the final concentration of 0 mug/mL, 0.0974 mug/mL and 0.3880 mug/mL, quantitative liquid medicine is taken and uniformly smeared on rice leaves, after the liquid medicine is dried, the rice sheath blight fungus cake is inoculated for 72 hours, each treatment is repeated for 3 times, the rice sheath blight fungus cake is placed in a 26-28 ℃ artificial climate box, and is subjected to moisturizing culture for 96 hours, the colony diameter is measured by adopting a crisscross method, the inhibition rate is calculated according to a formula (1), and the control effect is evaluated.
(3) Results and analysis
The imazalil and phenazine-1-carboxylic acid compound has control effect on rice sheath blight. The higher the concentration of imazalil and phenazine-1-carboxylic acid complex, the better the control effect (Table 10). When the concentration of the imazalil and phenazine-1-carboxylic acid compound is 0.3880 mug/mL, the control effect on rice sheath blight disease is best and is 2.23 times of the minimum concentration. The analysis of variance results show that: the prevention and treatment effects of different treatments on rice sheath blight diseases are remarkable. In a word, the study shows that the imazalil and phenazine-1-carboxylic acid compound has a good control effect on rice sheath blight, and can be used as a candidate drug for controlling rice sheath blight.
Table 10 in vitro leaf control of Rhizoctonia solani by compounding imazalil with phenazine-1-carboxylic acid
Example 5:
(1) Test material
Test pathogens used in this test: botrytis cinerea is provided by the plant pest biology and control major laboratory of Hunan university of agriculture, proc. Plant diseases and insect pests, hunan province.
The agent used in this experiment: 97% imazalil was purchased from national pharmaceutical group chemical company, inc; 92% of phenazine-1-carboxylic acid was developed by the agricultural microbiology laboratory of Hunan university; other analytical reagents, absolute ethanol, tween-80, acetone, glucose, agar strips, etc., were purchased from the biological sciences limited of wutai in the hunan.
Test medium: PDA culture medium, 200g potato, 20g glucose, 20g agar, pH value is natural, water is added to fix volume to 1L, and sterilization is carried out for 20min at 121 ℃ under 1 atmosphere for standby.
(2) In-vitro fruit control effect of imazalil and phenazine-1-carboxylic acid on strawberry gray mold
The imazalil and phenazine-1-carboxylic acid compound is formed into a liquid medicine with the final concentration of 0 mug/mL, 0.9693 mug/mL and 3.8772 mug/mL, quantitative liquid medicine is uniformly smeared on the strawberry fruit surface, after the strawberry fruit surface is dried in the air, the strawberry gray mold fungus cake is inoculated for 72 hours, each treatment is repeated for 3 times, the strawberry gray mold fungus cake is placed in a 26-28 ℃ artificial climate box for preserving moisture and culturing for 96 hours, the colony diameter is measured by adopting a crisscross method, the inhibition rate is calculated according to a formula (1), and the control effect is evaluated.
(3) Results and analysis
The test result shows that: the imazalil and phenazine-1-carboxylic acid compound has control effect on the gray mold of strawberries. The higher the concentration of imazalil and phenazine-1-carboxylic acid complex, the better the control of strawberry gray mold (Table 11). When the concentration of the imazalil and phenazine-1-carboxylic acid compound is 3.8772 mug/mL, the control effect on the gray mold of the strawberry is best and is 2.49 times of the minimum concentration. Notably, are: analysis of variance results indicated that: the differences between the different treatments are significant. Therefore, the imazalil and phenazine-1-carboxylic acid compound can be used as a bactericide for preventing and controlling the gray mold of strawberries.
Table 11 in vitro fruit control efficacy of imazalil and phenazine-1-carboxylic acid combination on strawberry gray mold
Example 6:
(1) Test material
Test pathogens used in this test: the pepper anthracnose germ (Colletotrichum capsici) is provided by the plant disease and pest biology and control major laboratory of the university of agriculture plant protection institute of Hunan province.
The agent used in this experiment: 97% imazalil was purchased from national pharmaceutical group chemical company, inc; 92% of phenazine-1-carboxylic acid was developed by the agricultural microbiology laboratory of Hunan university; other analytical reagents, absolute ethanol, tween-80, acetone, glucose, agar strips, etc., were purchased from the biological sciences limited of wutai in the hunan.
Test medium: PDA culture medium, 200g potato, 20g glucose, 20g agar, pH value is natural, water is added to fix volume to 1L, and sterilization is carried out for 20min at 121 ℃ under 1 atmosphere for standby.
(2) In-vitro fruit control effect of pepper anthracnose by compounding imazalil and phenazine-1-carboxylic acid
The imazalil and phenazine-1-carboxylic acid compound is formed into a liquid medicine with the final concentration of 0 mug/mL, 2.4829 mug/mL and 9.9316 mug/mL, quantitative liquid medicine is uniformly smeared on capsicum fruit surfaces, after the capsicum fruit surfaces are dried in the air, the capsicum anthracnose bacterial cakes are inoculated for 72 hours, each treatment is repeated for 3 times, the capsicum anthracnose bacterial cakes are placed in a 26-28 ℃ artificial climate box for preserving moisture and culturing for 96 hours, the colony diameter is measured by adopting a crisscross method, the inhibition rate is calculated according to a formula (1), and the prevention and treatment effect is evaluated.
(3) Results and analysis
The test results show that: the imazalil and phenazine-1-carboxylic acid compound has a certain control effect on pepper anthracnose. The higher the concentration of imazalil and phenazine-1-carboxylic acid complex, the better the control of pepper anthracnose (table 12). When the concentration of the imazalil and phenazine-1-carboxylic acid compound is 9.9316 mug/mL, the control effect on pepper anthracnose is best and is 2.64 times of the minimum concentration. Notably, are: analysis of variance results indicated that: the differences between the different treatments are significant. In conclusion, the imazalil and phenazine-1-carboxylic acid compound can be used as a standby medicament for preventing and controlling pepper anthracnose.
Table 12 anti-effects of imazalil and phenazine-1-carboxylic acid on in vitro fruits of Capsicum anthracnose
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Claims (10)
1. The bactericidal composition is characterized by comprising the following components in percentage by mass of 0.5-5: 1 or the sterilization composition comprises imazalil and phenazine-1-carboxylic acid in a mass ratio of 1:3.4-5.5.
2. The sterilizing composition according to claim 1, wherein the sterilizing composition comprises 1 to 4 by mass: 1 or the bactericidal composition comprises imazalil and phenazine-1-carboxylic acid according to the mass ratio of 1:3.5 to 5.5 of imazalil and phenazine-1-carboxylic acid, preferably the bactericidal composition comprises 2 to 4 of the following components in mass ratio: 1 and phenazine-1-carboxylic acid.
3. A bactericidal composition according to claim 2, characterized in that the EC of the bactericidal composition 50 0.08-0.6 mug/mL;
the pH value of the environment of the sterilizing composition in the use process is less than or equal to 7.
4. A bactericidal composition according to claim 2, characterized in that the total mass concentration of the bactericidal composition is 0.2-0.5 μg/mL, preferably the total mass concentration of the bactericidal composition is 0.3-0.4 μg/mL.
5. The sterilizing composition according to claim 1, wherein the sterilizing composition comprises 4 to 5 by mass: 1 and phenazine-1-carboxylic acid; or the bactericidal composition comprises imazalil and phenazine-1-carboxylic acid in a mass ratio of 1:3.4-4.5.
6. The bactericidal composition of claim 5, wherein the EC of the bactericidal composition 50 0.08-4.1 mug/mL;
the pH value of the environment of the sterilizing composition in the use process is less than or equal to 7.
7. A bactericidal composition according to claim 5, wherein the total mass concentration of the bactericidal composition is 3.6-4.1 μg/mL, preferably the total mass concentration of the bactericidal composition is 3.8-4.0 μg/mL.
8. The sterilizing composition according to claim 1, wherein the sterilizing composition comprises 0.5 to 3 by mass: 1 and phenazine-1-carboxylic acid, preferably, the bactericidal composition comprises the components with the mass ratio of 0.5-1.5: 1 and phenazine-1-carboxylic acid.
9. The antiseptic composition of claim 8 wherein the EC of the antiseptic composition 50 2.4-2.8 mug/mL;
the pH value of the environment of the sterilizing composition in the use process is less than or equal to 7.
10. A bactericidal composition according to claim 8, wherein the total mass concentration of the bactericidal composition is 8-12 μg/mL, preferably the total mass concentration of the bactericidal composition is 9-10 μg/mL.
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